Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008549

Physical Location & Seq

Genomic Coordinates Reverse (-)

26429938 .. 26434995

Gene Structure 5058 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1172800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGGGCGATCTTCGGGATTGGTCGCCTGAACCGAACGGTGCCGTGTTGGAGGAGACGGAGAGGGCTTCTTCGTCCTCGTCGTTGTCGTCATCACCTTCGTCCCAATCTTCATCGAATCAACAGGCCGCGGCGGCTGCGTCTGCGTCTGCGGCGGCGGCCATTAGCGCCGAGTACTGGCTGCGGGCGGAGGAGGCGACGCAGCGCGTCATAGCGCAGGTTCAGACCACTGACGTGTCCGAGAGGAGGAGGAAGGCGGTCATTGATTACGTTCAGAGGCTCATCAAAAGTTCTCTTGGTTGCGAGGTTTTCCCATTTGGGTCTGTACCTCTAAAGACCTATTTGCCTGATGGAGATATTGATTTGACTGCTTTTGGTGGTATGAATGTTGAGGAGGCGCTGGCCAATGACGTCTGCGCTGTCCTTGAAAGGGAAGATAAAAATATGGCTGCTGAGTTTATGGTTAAAGATGTGCAGTTGATTCGGGCAGAGGTCAAACTCGTAAAGTGCCTTGTGCAAAATATTGTGGTCGATATTTCCTTCAATCAGTTAGGAGGGCTATGCACACTATGCTTTCTTGAGCAGGTTGATCGCCTCATTGGCAAAGACCATCTTTTTAAACGCAGTATTATACTAATTAAGGCCTGGTGCTATTATGAGAGCCGGATTCTTGGTGCCCATCATGGTTTGATTTCGACATATGCTTTGGAGACTTTGGTTCTGTATATCTTCCACCTCTTCCATTCATCTTTGAATGGTCCTCTAGCGGTCCTATATAAATTCTTGGACTACTTTAGTAAATTTGATTGGGATAGCTATTGTATCAGCTTGAGTGGTCCTGTTCGTATATCTTCTCTTCCAGAACTCTTGGCTGAGACGCCAGAAAATGGTGGCGGTGATCTATTACTGAGTAATGATTTTCTCAAGGGATGTGTGGAAAGGTTCTCAGTTCCTTCAAGAGGATATGAGACAAACCATAGAACATTTCAACCAAAGCATCTTAACATAGTTGATCCGCTTAAAGACAATAACAACCTTGGCCGAAGTGTGAGTAAAGGGAACTTTTACCGAATAAGAAGTGCTTTCACATATGGTGCTCGTAAACTAGGGCGCTTTCTTTCTCAGCCAGAAGTAAACGTAGATGATGAGATCCGAAAGTTTTTCGCTAACACATTGGACAGACATGGAGGCGGACAAAGGCCTGATGTCCAAGATCCTGTCCCATGGTCCATAAATGATGGGTATGGTAGTGTGCCATTATTGGTAGGTACAGAGTCACAAGAAGATCAGACAATTCATGAGTCGGAATCTGCCTATTCTATTGGCGTGATTGGGGAATGTGGGTTAAATCATGATTCATGGAATGGTAAAGTTGCAAGTGGTCATATACCTGGTCAAGGTGTGACTAGACCACATGAAAATGATATGAAACCTGATCAAGATGTTAATGGAGCACATGAAAGCATGAAGGTTGCATCCCCAACAATGTTATTGGACGAGGACAGTTCCCCAAATGGAATTGCTGTTTCTGAAAACCGTCTTATAGGGGATGCAAAAGACCTTGCTACCTCCAGATTTCAAGGTCTTATAATTTCAAATGATGCTCAAAGTCCTTTGCCGTCCAGTTGTGAAGTGAGTATATCTCTTTTAGGTAAAGTACAGCATGCACCTCATCTCTGTTCCTCTCACTCGTCAACTGAAAATGGAGACATTAGCACTGGAAACCAAGATCAGCTATTGCCAGAAAGCTTTGGTTCAGCTGATACTTTGGTCAGTAACCAGGATGAGAATCAGTTGGGTGGTAATCAGGAAGTTCCATCTCCTGTTGGATCTAAGCGCCATCCGTCACGGTTGAGCTCTGTTGTGTGTTCGTCTGAAGACTTCTATCCTAGTTATTATCCAATGTCAAGTGGCACTACTGGAAGTCCCAAACCTTCTAACTATGTAACAGATCTGAGTGGGGATTACGATAGTCATTTACGTAGTCTGAACTATGGGCGACGGTGTTATGATTGTGAGCTAAATGTGGCGATTCCTCCCATGGCGCCACCTCCGGTGCACCCACAGTTTCAAGGCAAGAAGCCATGGGATGTAACTCGAGAGTCAGTGCAGCACAGGCAGAATGCATTTTCCCAGATGAATGTCAATGGTGTTGTCCCTAGACCACCATTCTATCCGATGAACCCACCAATGTTGCCTAACGGCACAGGCTTTGGCAGGGAGGAGTTGCCAAAGCCACGAGGAACCGGAACTTACTTTCCCAATACGAACCATTTCAGAGATAGGCCTGTGACGCCAAGGGGAAGAAATCAAGCACCGCCAAAGTCTCCTCGTAACAATGGCCATGCCATGATACCACCTCTAGAGAATTATACGTCTGACAGAAGCAGTCGTGATATGTCACAACTACAAATGTCTCTTCATAAAGGTGGGGGGAAATCTGGGTCTGCAGATAGCTCCACTGGTTCCCCTAGAAGGAAAGTACACCCTAATGTGAATGGTTCAATGCATTCCTCTGAGAAAGTTATAGAATTTGGGGCATTGGGGCATGCCCCACAAGAGGGACATGCAAGTGGCAAACATACAAATGCTGGCTCATCGTTCGTTCAAAACTCGGGTAGTGGCCAGTCATCACCAAAGATGCAGTTGACAAAAGATGAGTTGGTTACGGATCCTAACAGGATTGCTCCACAATCATACCGTTTGAAAGATGAAGAAGATTTCCCACCTTTATCGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

913

Amino Acids

99.68

Weight (kDa)

5.84

Isoelectric Point (pI)

49.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	66 - 186	1.8e-08	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	199 - 391	1.1e-98	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008549)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51620 AT3G51620 AT3G51620
fragaria_vesca	FvH4_6g10110 FvH4_6g10110 FvH4_6g10110 FvH4_6g10110 FvH4_6g10110
malus_domestica	MD04G1172800.v1.1 MD12G1186100.v1.1
prunus_persica	Prupe.6G291600_v2.0.a1
pyrus_communis	pycom04g15270 pycom12g17470
rosa_chinensis	RchiOBHm_Chr3g0460811
rosa_laevigata	RLG00000024979
rosa_multiflora	Rmu_sc0007678.1_g000014
rosa_roxburghii	Rroxscaffold_6G00419200
rosa_rugosa	Rorug03G0046500
rosa_samantha	Rh3AG103600 Rh3BG107300 Rh3CG109200 Rh3DG109100
rosa_wichuraiana	Rw3G008820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1586
AatII	GACGTC	1 cut(s)	411
Acc36I	ACCTGC	2 cut(s)	205, 571
AccB1I	GGYRCC	3 cut(s)	38, 671, 2041
AccB7I	CCANNNNNTGG	1 cut(s)	884
AccII	CGCG	2 cut(s)	128, 204
AclWI	GGATC	6 cut(s)	1004, 1141, 1205, 1834, 2663, 2676
AcoI	YGGCCR	5 cut(s)	156, 399, 1036, 2338, 2620
AcsI	RAATTY	3 cut(s)	776, 797, 2528
AcuI	CTGAAG	1 cut(s)	1893
AcyI	GRCGYC	4 cut(s)	408, 875, 2042, 2291
AfaI	GTAC	5 cut(s)	173, 324, 1267, 1656, 2481
AfiI	CCNNNNNNNGG	9 cut(s)	174, 243, 427, 884, 956, 1394, 2472, 2557, 2558
AflIII	ACRYGT	1 cut(s)	231
AjiI	CACGTC	1 cut(s)	232
AjnI	CCWGG	3 cut(s)	641, 1387, 1776
AleI	CACNNNNGTG	1 cut(s)	230
AloI	GAACNNNNNNTCC	2 cut(s)	2239, 2271
AluBI	AGCT	8 cut(s)	813, 825, 1732, 1746, 1757, 1854, 2017, 2454
AluI	AGCT	8 cut(s)	813, 825, 1732, 1746, 1757, 1854, 2017, 2454
Alw21I	GWGCWC	4 cut(s)	1096, 1453, 1856, 2058
Alw26I	GTCTC	7 cut(s)	47, 701, 866, 959, 1698, 2328, 2418
Alw44I	GTGCAC	1 cut(s)	2054
AlwI	GGATC	6 cut(s)	1004, 1141, 1205, 1834, 2663, 2676
Ama87I	CYCGRG	2 cut(s)	2094, 2611
ApaLI	GTGCAC	1 cut(s)	2054
ApeKI	GCWGC	5 cut(s)	134, 178, 199, 446, 2107
ApoI	RAATTY	3 cut(s)	776, 797, 2528
ArsI	GACNNNNNNTTYG	2 cut(s)	685, 717
Asp700I	GAANNNNTTC	1 cut(s)	2270
AspLEI	GCGC	8 cut(s)	167, 204, 214, 397, 416, 1110, 1836, 2044
AspS9I	GGNCC	4 cut(s)	755, 766, 833, 1224
AsuHPI	GGTGA	3 cut(s)	84, 905, 2622
AvaI	CYCGRG	2 cut(s)	2094, 2611
AvaII	GGWCC	4 cut(s)	755, 766, 833, 1224
BaeGI	GKGCMC	2 cut(s)	676, 2058
BaeI	ACNNNNGTAYC	2 cut(s)	1752, 1785
BalI	TGGCCA	3 cut(s)	401, 2340, 2622
BamHI	GGATCC	1 cut(s)	2668
BanI	GGYRCC	3 cut(s)	38, 671, 2041
BanII	GRGCYC	1 cut(s)	1856
BauI	CACGAG	1 cut(s)	2235
BbsI	GAAGAC	1 cut(s)	1881
Bbv12I	GWGCWC	4 cut(s)	1096, 1453, 1856, 2058
BbvI	GCAGC	5 cut(s)	121, 165, 211, 433, 2119
BccI	CCATC	6 cut(s)	341, 615, 684, 1229, 1822, 1845
BceAI	ACGGC	3 cut(s)	26, 1600, 2215
BcgI	CGANNNNNNTGC	4 cut(s)	461, 495, 569, 603
BciT130I	CCWGG	3 cut(s)	643, 1389, 1778
BclI	TGATCA	1 cut(s)	1432
BcoDI	GTCTC	7 cut(s)	47, 701, 866, 959, 1698, 2328, 2418
BfaI	CTAG	7 cut(s)	761, 1103, 1404, 1887, 2157, 2360, 2469
BfmI	CTRYAG	1 cut(s)	2445
BfoI	RGCGCY	5 cut(s)	168, 398, 1111, 1837, 2045
BfuAI	ACCTGC	2 cut(s)	205, 571
BglI	GCCNNNNNGGC	2 cut(s)	131, 597
BglII	AGATCT	1 cut(s)	1948
BmcAI	AGTACT	1 cut(s)	173
Bme1390I	CCNGG	3 cut(s)	643, 1389, 1778
Bme18I	GGWCC	4 cut(s)	755, 766, 833, 1224
BmeT110I	CYCGRG	2 cut(s)	2094, 2611
BmgBI	CACGTC	1 cut(s)	232
BmgT120I	GGNCC	4 cut(s)	755, 766, 833, 1224
BmiI	GGNNCC	6 cut(s)	40, 673, 2043, 2242, 2464, 2670
BmrFI	CCNGG	3 cut(s)	643, 1389, 1778
BmsI	GCATC	5 cut(s)	1003, 1481, 1537, 1588, 2628
BpiI	GAAGAC	1 cut(s)	1881
BplI	GAGNNNNNCTC	2 cut(s)	1624, 1656
BpmI	CTGGAG	1 cut(s)	1552
BpuEI	CTTGAG	3 cut(s)	596, 847, 905
BsaAI	YACGTR	1 cut(s)	1979
BsaBI	GATNNNNATC	1 cut(s)	1785
BsaHI	GRCGYC	4 cut(s)	408, 875, 2042, 2291
BsaJI	CCNNGG	6 cut(s)	126, 1033, 1220, 2037, 2081, 2294
BsaWI	WCCGGW	2 cut(s)	2050, 2243
Bsc4I	CCNNNNNNNGG	9 cut(s)	174, 243, 427, 884, 956, 1394, 2472, 2557, 2558
Bse1I	ACTGG	6 cut(s)	179, 1620, 1720, 1921, 2464, 2623
Bse8I	GATNNNNATC	1 cut(s)	1785
BseBI	CCWGG	3 cut(s)	643, 1389, 1778
BseDI	CCNNGG	6 cut(s)	126, 1033, 1220, 2037, 2081, 2294
BseGI	GGATG	6 cut(s)	932, 1472, 1552, 1786, 1837, 2092
BseJI	GATNNNNATC	1 cut(s)	1785
BseLI	CCNNNNNNNGG	9 cut(s)	174, 243, 427, 884, 956, 1394, 2472, 2557, 2558
BseMII	CTCAG	7 cut(s)	441, 861, 896, 957, 1133, 1943, 2505
BseNI	ACTGG	6 cut(s)	179, 1620, 1720, 1921, 2464, 2623
BseRI	GAGGAG	7 cut(s)	65, 203, 256, 259, 404, 2234, 2316
BseSI	GKGCMC	2 cut(s)	676, 2058
BseXI	GCAGC	5 cut(s)	121, 165, 211, 433, 2119
BsgI	GTGCAG	2 cut(s)	491, 2126
Bsh1236I	CGCG	2 cut(s)	128, 204
BshNI	GGYRCC	3 cut(s)	38, 671, 2041
BsiHKAI	GWGCWC	4 cut(s)	1096, 1453, 1856, 2058
BsiHKCI	CYCGRG	2 cut(s)	2094, 2611
BsiSI	CCGG	3 cut(s)	661, 2051, 2244
BslFI	GGGAC	5 cut(s)	85, 1202, 1908, 2138, 2574
BslI	CCNNNNNNNGG	9 cut(s)	174, 243, 427, 884, 956, 1394, 2472, 2557, 2558
BsmAI	GTCTC	7 cut(s)	47, 701, 866, 959, 1698, 2328, 2418
BsmBI	CGTCTC	2 cut(s)	47, 866
BsmFI	GGGAC	5 cut(s)	85, 1202, 1908, 2138, 2574
BsmI	GAATGC	2 cut(s)	2125, 2506
BsoBI	CYCGRG	2 cut(s)	2094, 2611
Bsp1286I	GDGCHC	5 cut(s)	676, 1096, 1453, 1856, 2058
Bsp19I	CCATGG	3 cut(s)	1220, 2037, 2081
BspCNI	CTCAG	7 cut(s)	442, 862, 897, 956, 1132, 1944, 2506
BspFNI	CGCG	2 cut(s)	128, 204
BspHI	TCATGA	2 cut(s)	1294, 1348
BspLI	GGNNCC	6 cut(s)	40, 673, 2043, 2242, 2464, 2670
BspMAI	CTGCAG	1 cut(s)	2449
BspMI	ACCTGC	2 cut(s)	205, 571
BspPI	GGATC	6 cut(s)	1004, 1141, 1205, 1834, 2663, 2676
BspT107I	GGYRCC	3 cut(s)	38, 671, 2041
BsrI	ACTGG	6 cut(s)	179, 1620, 1720, 1921, 2464, 2623
BssECI	CCNNGG	6 cut(s)	126, 1033, 1220, 2037, 2081, 2294
BssNI	GRCGYC	4 cut(s)	408, 875, 2042, 2291
BssSI	CACGAG	1 cut(s)	2235
BssT1I	CCWWGG	5 cut(s)	1033, 1220, 2037, 2081, 2294
Bst2BI	CACGAG	1 cut(s)	2235
Bst2UI	CCWGG	3 cut(s)	643, 1389, 1778
Bst4CI	ACNGT	7 cut(s)	38, 1502, 1535, 1848, 2001, 2064, 2699
Bst6I	CTCTTC	3 cut(s)	740, 858, 2421
BstACI	GRCGYC	4 cut(s)	408, 875, 2042, 2291
BstAPI	GCANNNNNTGC	1 cut(s)	567
BstBAI	YACGTR	1 cut(s)	1979
BstC8I	GCNNGC	5 cut(s)	183, 399, 1662, 2547, 2590
BstDEI	CTNAG	8 cut(s)	450, 870, 905, 943, 1119, 1830, 1952, 2514
BstDSI	CCRYGG	4 cut(s)	126, 1220, 2037, 2081
BstENI	CCTNNNNNAGG	2 cut(s)	954, 1392
BstF5I	GGATG	6 cut(s)	932, 1472, 1552, 1786, 1837, 2092
BstFNI	CGCG	2 cut(s)	128, 204
BstH2I	RGCGCY	5 cut(s)	168, 398, 1111, 1837, 2045
BstHHI	GCGC	8 cut(s)	167, 204, 214, 397, 416, 1110, 1836, 2044
BstMAI	GTCTC	7 cut(s)	47, 701, 866, 959, 1698, 2328, 2418
BstNI	CCWGG	3 cut(s)	643, 1389, 1778
BstNSI	RCATGY	3 cut(s)	1664, 2549, 2567
BstSCI	CCNGG	3 cut(s)	641, 1387, 1776
BstSFI	CTRYAG	1 cut(s)	2445
BstSLI	GKGCMC	2 cut(s)	676, 2058
BstSNI	TACGTA	1 cut(s)	1979
BstUI	CGCG	2 cut(s)	128, 204
BstV1I	GCAGC	5 cut(s)	121, 165, 211, 433, 2119
BstV2I	GAAGAC	1 cut(s)	1881
BstX2I	RGATCY	5 cut(s)	1146, 1210, 1826, 1948, 2668
BstYI	RGATCY	5 cut(s)	1146, 1210, 1826, 1948, 2668
BtgI	CCRYGG	4 cut(s)	126, 1220, 2037, 2081
BtrI	CACGTC	1 cut(s)	232
BtsCI	GGATG	6 cut(s)	932, 1472, 1552, 1786, 1837, 2092
BtsIMutI	CAGTG	4 cut(s)	225, 1713, 2109, 2457
BveI	ACCTGC	2 cut(s)	205, 571
Cac8I	GCNNGC	5 cut(s)	183, 399, 1662, 2547, 2590
CciI	TCATGA	2 cut(s)	1294, 1348
CfoI	GCGC	8 cut(s)	167, 204, 214, 397, 416, 1110, 1836, 2044
Cfr13I	GGNCC	4 cut(s)	755, 766, 833, 1224
Cfr42I	CCGCGG	1 cut(s)	129
CseI	GACGC	6 cut(s)	126, 132, 193, 205, 883, 2299
CsiI	ACCWGGT	1 cut(s)	1387
Csp6I	GTAC	5 cut(s)	172, 323, 1266, 1655, 2480
CspCI	CAANNNNNGTGG	2 cut(s)	2172, 2207
CviQI	GTAC	5 cut(s)	172, 323, 1266, 1655, 2480
DdeI	CTNAG	8 cut(s)	450, 870, 905, 943, 1119, 1830, 1952, 2514
DinI	GGCGCC	1 cut(s)	2043
DraI	TTTAAA	1 cut(s)	616
EaeI	YGGCCR	5 cut(s)	156, 399, 1036, 2338, 2620
Eam1104I	CTCTTC	3 cut(s)	740, 858, 2421
EarI	CTCTTC	3 cut(s)	740, 858, 2421
EciI	GGCGGA	2 cut(s)	200, 1203
Ecl136II	GAGCTC	1 cut(s)	1854
Eco105I	TACGTA	1 cut(s)	1979
Eco130I	CCWWGG	5 cut(s)	1033, 1220, 2037, 2081, 2294
Eco147I	AGGCCT	3 cut(s)	641, 1198, 2284
Eco24I	GRGCYC	1 cut(s)	1856
Eco47I	GGWCC	4 cut(s)	755, 766, 833, 1224
Eco53kI	GAGCTC	1 cut(s)	1854
Eco57I	CTGAAG	1 cut(s)	1893
Eco88I	CYCGRG	2 cut(s)	2094, 2611
EcoICRI	GAGCTC	1 cut(s)	1854
EcoNI	CCTNNNNNAGG	2 cut(s)	954, 1392
EcoRII	CCWGG	3 cut(s)	641, 1387, 1776
EcoT14I	CCWWGG	5 cut(s)	1033, 1220, 2037, 2081, 2294
EcoT22I	ATGCAT	2 cut(s)	2125, 2508
EcoT38I	GRGCYC	1 cut(s)	1856
EgeI	GGCGCC	1 cut(s)	2043
EheI	GGCGCC	1 cut(s)	2043
ErhI	CCWWGG	5 cut(s)	1033, 1220, 2037, 2081, 2294
Esp3I	CGTCTC	2 cut(s)	47, 866
FalI	AAGNNNNNCTT	4 cut(s)	594, 626, 1063, 1095
FaqI	GGGAC	5 cut(s)	85, 1202, 1908, 2138, 2574
FauI	CCCGC	1 cut(s)	174
FauNDI	CATATG	2 cut(s)	697, 1087
FbaI	TGATCA	1 cut(s)	1432
FokI	GGATG	6 cut(s)	939, 1459, 1559, 1793, 1824, 2099
FriOI	GRGCYC	1 cut(s)	1856
FspBI	CTAG	7 cut(s)	761, 1103, 1404, 1887, 2157, 2360, 2469
GlaI	GCGC	8 cut(s)	166, 203, 213, 396, 415, 1109, 1835, 2043
GsuI	CTGGAG	1 cut(s)	1552
HaeII	RGCGCY	5 cut(s)	168, 398, 1111, 1837, 2045
HapII	CCGG	3 cut(s)	661, 2051, 2244
HgaI	GACGC	6 cut(s)	126, 132, 193, 205, 883, 2299
HhaI	GCGC	8 cut(s)	167, 204, 214, 397, 416, 1110, 1836, 2044
Hin1I	GRCGYC	4 cut(s)	408, 875, 2042, 2291
Hin6I	GCGC	8 cut(s)	165, 202, 212, 395, 414, 1108, 1834, 2042
HinP1I	GCGC	8 cut(s)	165, 202, 212, 395, 414, 1108, 1834, 2042
HincII	GTYRAC	2 cut(s)	1692, 2646
HindII	GTYRAC	2 cut(s)	1692, 2646
HindIII	AAGCTT	1 cut(s)	1744
HpaII	CCGG	3 cut(s)	661, 2051, 2244
HphI	GGTGA	3 cut(s)	84, 905, 2622
Hpy166II	GTNNAC	6 cut(s)	1100, 1132, 1692, 2056, 2482, 2646
Hpy8I	GTNNAC	6 cut(s)	1100, 1132, 1692, 2056, 2482, 2646
Hpy99I	CGWCG	3 cut(s)	82, 199, 2001
HpyAV	CCTTC	7 cut(s)	105, 244, 547, 960, 1459, 1941, 2466
HpyCH4III	ACNGT	7 cut(s)	38, 1502, 1535, 1848, 2001, 2064, 2699
HpyCH4IV	ACGT	6 cut(s)	231, 267, 408, 1134, 1978, 2372
HpyF3I	CTNAG	8 cut(s)	450, 870, 905, 943, 1119, 1830, 1952, 2514
HpySE526I	ACGT	6 cut(s)	231, 267, 408, 1134, 1978, 2372
Hsp92I	GRCGYC	4 cut(s)	408, 875, 2042, 2291
HspAI	GCGC	8 cut(s)	165, 202, 212, 395, 414, 1108, 1834, 2042
KasI	GGCGCC	1 cut(s)	2041
Ksp22I	TGATCA	1 cut(s)	1432
KspI	CCGCGG	1 cut(s)	129
LmnI	GCTCC	3 cut(s)	1448, 2459, 2689
Lsp1109I	GCAGC	5 cut(s)	121, 165, 211, 433, 2119
LweI	GCATC	5 cut(s)	1003, 1481, 1537, 1588, 2628
MabI	ACCWGGT	1 cut(s)	1387
MaeI	CTAG	7 cut(s)	761, 1103, 1404, 1887, 2157, 2360, 2469
MaeII	ACGT	6 cut(s)	231, 267, 408, 1134, 1978, 2372
MflI	RGATCY	5 cut(s)	1146, 1210, 1826, 1948, 2668
MhlI	GDGCHC	5 cut(s)	676, 1096, 1453, 1856, 2058
MlsI	TGGCCA	3 cut(s)	401, 2340, 2622
MluCI	AATT	8 cut(s)	633, 776, 797, 1290, 1515, 1587, 2365, 2528
MluNI	TGGCCA	3 cut(s)	401, 2340, 2622
Mly113I	GGCGCC	1 cut(s)	2042
MlyI	GAGTC	3 cut(s)	1280, 1307, 2108
MmeI	TCCRAC	3 cut(s)	27, 1281, 1804
Mox20I	TGGCCA	3 cut(s)	401, 2340, 2622
Mph1103I	ATGCAT	2 cut(s)	2125, 2508
MroXI	GAANNNNTTC	1 cut(s)	2270
MscI	TGGCCA	3 cut(s)	401, 2340, 2622
MseI	TTAA	7 cut(s)	462, 615, 636, 999, 1017, 1343, 1443
MslI	CAYNNNNRTG	6 cut(s)	230, 1416, 2424, 2487, 2568, 2583
Msp20I	TGGCCA	3 cut(s)	401, 2340, 2622
MspA1I	CMGCKG	2 cut(s)	128, 1757
MspI	CCGG	3 cut(s)	661, 2051, 2244
MspR9I	CCNGG	3 cut(s)	643, 1389, 1778
Mva1269I	GAATGC	2 cut(s)	2125, 2506
MvaI	CCWGG	3 cut(s)	643, 1389, 1778
MvnI	CGCG	2 cut(s)	128, 204
NarI	GGCGCC	1 cut(s)	2042
NcoI	CCATGG	3 cut(s)	1220, 2037, 2081
NdeI	CATATG	2 cut(s)	697, 1087
NlaIV	GGNNCC	6 cut(s)	40, 673, 2043, 2242, 2464, 2670
NmeAIII	GCCGAG	1 cut(s)	193
NmuCI	GTSAC	5 cut(s)	1272, 1399, 1842, 2287, 2397
NsiI	ATGCAT	2 cut(s)	2125, 2508
NspI	RCATGY	3 cut(s)	1664, 2549, 2567
OliI	CACNNNNGTG	1 cut(s)	230
PaeI	GCATGC	2 cut(s)	1664, 2549
PaeR7I	CTCGAG	1 cut(s)	2094
PagI	TCATGA	2 cut(s)	1294, 1348
PceI	AGGCCT	3 cut(s)	641, 1198, 2284
PcsI	WCGNNNNNNNCGW	3 cut(s)	29, 77, 83
PctI	GAATGC	2 cut(s)	2125, 2506
PdmI	GAANNNNTTC	1 cut(s)	2270
PfeI	GAWTC	7 cut(s)	115, 478, 664, 1304, 1352, 1786, 2029
PflMI	CCANNNNNTGG	1 cut(s)	884
PleI	GAGTC	3 cut(s)	1279, 1306, 2107
PluTI	GGCGCC	1 cut(s)	2045
PpsI	GAGTC	3 cut(s)	1279, 1306, 2107
Ppu21I	YACGTR	1 cut(s)	1979
PsiI	TTATAA	1 cut(s)	1586
Psp124BI	GAGCTC	1 cut(s)	1856
Psp6I	CCWGG	3 cut(s)	641, 1387, 1776
PspGI	CCWGG	3 cut(s)	641, 1387, 1776
PspN4I	GGNNCC	6 cut(s)	40, 673, 2043, 2242, 2464, 2670
PspPI	GGNCC	4 cut(s)	755, 766, 833, 1224
PstI	CTGCAG	1 cut(s)	2449
PsuI	RGATCY	5 cut(s)	1146, 1210, 1826, 1948, 2668
PvuII	CAGCTG	1 cut(s)	1757
RsaI	GTAC	5 cut(s)	173, 324, 1267, 1656, 2481
RsaNI	GTAC	5 cut(s)	172, 323, 1266, 1655, 2480
RseI	CAYNNNNRTG	6 cut(s)	230, 1416, 2424, 2487, 2568, 2583
SacI	GAGCTC	1 cut(s)	1856
SacII	CCGCGG	1 cut(s)	129
SaqAI	TTAA	7 cut(s)	462, 615, 636, 999, 1017, 1343, 1443
Sau96I	GGNCC	4 cut(s)	755, 766, 833, 1224
ScaI	AGTACT	1 cut(s)	173
SchI	GAGTC	3 cut(s)	1280, 1307, 2108
ScrFI	CCNGG	3 cut(s)	643, 1389, 1778
SduI	GDGCHC	5 cut(s)	676, 1096, 1453, 1856, 2058
SexAI	ACCWGGT	1 cut(s)	1387
SfaNI	GCATC	5 cut(s)	1003, 1481, 1537, 1588, 2628
SfcI	CTRYAG	1 cut(s)	2445
SfoI	GGCGCC	1 cut(s)	2043
Sfr274I	CTCGAG	1 cut(s)	2094
Sfr303I	CCGCGG	1 cut(s)	129
SgrBI	CCGCGG	1 cut(s)	129
SinI	GGWCC	4 cut(s)	755, 766, 833, 1224
SlaI	CTCGAG	1 cut(s)	2094
SmiMI	CAYNNNNRTG	6 cut(s)	230, 1416, 2424, 2487, 2568, 2583
SmlI	CTYRAG	4 cut(s)	575, 826, 920, 2094
SmoI	CTYRAG	4 cut(s)	575, 826, 920, 2094
SnaBI	TACGTA	1 cut(s)	1979
SphI	GCATGC	2 cut(s)	1664, 2549
Sse9I	AATT	8 cut(s)	633, 776, 797, 1290, 1515, 1587, 2365, 2528
SseBI	AGGCCT	3 cut(s)	641, 1198, 2284
SspDI	GGCGCC	1 cut(s)	2041
SspI	AATATT	1 cut(s)	520
SspMI	CTAG	7 cut(s)	761, 1103, 1404, 1887, 2157, 2360, 2469
SstI	GAGCTC	1 cut(s)	1856
StuI	AGGCCT	3 cut(s)	641, 1198, 2284
StyD4I	CCNGG	3 cut(s)	641, 1387, 1776
StyI	CCWWGG	5 cut(s)	1033, 1220, 2037, 2081, 2294
TaaI	ACNGT	7 cut(s)	38, 1502, 1535, 1848, 2001, 2064, 2699
TaiI	ACGT	6 cut(s)	234, 270, 411, 1137, 1981, 2375
TaqI	TCGA	4 cut(s)	113, 528, 692, 2095
TasI	AATT	8 cut(s)	633, 776, 797, 1290, 1515, 1587, 2365, 2528
TatI	WGTACW	3 cut(s)	171, 1654, 2479
TauI	GCSGC	6 cut(s)	128, 131, 134, 152, 155, 158
TfiI	GAWTC	7 cut(s)	115, 478, 664, 1304, 1352, 1786, 2029
Tru1I	TTAA	7 cut(s)	462, 615, 636, 999, 1017, 1343, 1443
Tru9I	TTAA	7 cut(s)	462, 615, 636, 999, 1017, 1343, 1443
TscAI	CASTG	4 cut(s)	232, 1720, 2109, 2464
TseFI	GTSAC	5 cut(s)	1272, 1399, 1842, 2287, 2397
TseI	GCWGC	5 cut(s)	134, 178, 199, 446, 2107
Tsp45I	GTSAC	5 cut(s)	1272, 1399, 1842, 2287, 2397
TspGWI	ACGGA	3 cut(s)	71, 1830, 2681
TspRI	CASTG	4 cut(s)	232, 1720, 2109, 2464
Van91I	CCANNNNNTGG	1 cut(s)	884
VneI	GTGCAC	1 cut(s)	2054
VpaK11BI	GGWCC	4 cut(s)	755, 766, 833, 1224
XagI	CCTNNNNNAGG	2 cut(s)	954, 1392
XapI	RAATTY	3 cut(s)	776, 797, 2528
XbaI	TCTAGA	1 cut(s)	2359
XceI	RCATGY	3 cut(s)	1664, 2549, 2567
XhoI	CTCGAG	1 cut(s)	2094
XmnI	GAANNNNTTC	1 cut(s)	2270
XspI	CTAG	7 cut(s)	761, 1103, 1404, 1887, 2157, 2360, 2469
ZraI	GACGTC	1 cut(s)	409
ZrmI	AGTACT	1 cut(s)	173
Zsp2I	ATGCAT	2 cut(s)	2125, 2508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.