MD12G1186200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013517
Physical Location & Seq
Forward (+)
26786446 .. 26789321
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1186200.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGAGGGAACTGGAAGGAGATCGAGATTCTCACTCCTGCTTCTTCCTCTGCTGATATCTCTCTCAATTCCCTGCATATCTGCCGCTTACAAGCCAGGCGACATCGTTCCCATGAGCAAGATGGGCCTTTATCACTCTATGAAAACCGAATGGCATGATATGATCGGTCGACATTGCCCAATATTTGCTGTCTATCGTGAGGTGTTAGTTCCTATAGCGAAGCCGATGGGTTATACAGGAGCTGATGCTTATAAGATATCATTTCAAGTTGGGAGAGAAAAGTTTTTAATACCGTGGCTTTTTGTGATAAATCGTAAAAGTTCAGAGGTCCCAATGATTGATGTCCATTTGAGGTACTCGGGAAGTGATTTGCATGGTGTCACTGCTAAAGTTGTGGATATGCCTCACCACTACTTAGAAATCCATCCAGATATTCGCAAACAATTTTGGGATGCTCAGCACTGGCCAAAGCATGTGCTGGTCAGATATACATGGGAGGAGCAATCAGAGATAGACGTGACTTCTGGATTTTATGTACTGTTTGGATCAGGTCTCATGCTTTCTTTTATTCTGTCGATATACATTTTGCAATCATCACGGGACAAGTTAGCAAGGTTTGTGAGGGAGAGAGTTGCAGAAAGCAACATGCCTGCTGGAGGTGTGGCAAAGGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.74

Weight (kDa)

9.0

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013517)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51610
fragaria_vesca FvH4_6g10100
malus_domestica MD04G1172900.v1.1 MD11G1271600.v1.1 MD12G1088300.v1.1 MD12G1186200.v1.1
prunus_persica Prupe.6G291700_v2.0.a1
pyrus_communis pycom04g15280
rosa_chinensis RchiOBHm_Chr3g0460801
rosa_laevigata RLG00000024980
rosa_multiflora Rmu_sc0007678.1_g000016
rosa_roxburghii Rroxscaffold_6G00419210
rosa_rugosa Rorug03G0046500
rosa_samantha Rh3AG103500 Rh3BG107200 Rh3CG109100 Rh3DG109000
rosa_wichuraiana Rw3G008810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
AccI GTMKAC 1 cut(s) 169
AciI CCGC 1 cut(s) 84
AclWI GGATC 1 cut(s) 553
AcoI YGGCCR 1 cut(s) 464
AfaI GTAC 2 cut(s) 356, 537
AfiI CCNNNNNNNGG 1 cut(s) 656
AgsI TTSAA 2 cut(s) 266, 674
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 557
AlwI GGATC 1 cut(s) 553
AlwNI CAGNNNCTG 1 cut(s) 242
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 2 cut(s) 124, 464
AspS9I GGNCC 2 cut(s) 124, 328
AsuHPI GGTGA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 328
BalI TGGCCA 1 cut(s) 466
BccI CCATC 3 cut(s) 115, 220, 432
BciT130I CCWGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 213
BisI GCNGC 1 cut(s) 84
BlpI GCTNAGC 1 cut(s) 456
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 2 cut(s) 124, 328
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 2 cut(s) 235, 442
BpmI CTGGAG 1 cut(s) 675
Bpu1102I GCTNAGC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 16, 467
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 2 cut(s) 424, 457
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 16, 467
BseRI GAGGAG 1 cut(s) 512
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 126, 466
BsiEI CGRYCG 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 358
BslFI GGGAC 2 cut(s) 314, 614
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 1 cut(s) 557
BsmFI GGGAC 2 cut(s) 314, 614
BsnI GGCC 2 cut(s) 126, 466
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 358
Bsp143I GATC 3 cut(s) 20, 162, 545
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 1 cut(s) 84
BspANI GGCC 2 cut(s) 126, 466
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 172
BsrI ACTGG 2 cut(s) 16, 467
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 3 cut(s) 20, 162, 545
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 294, 540
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 2 cut(s) 415, 456
BstDSI CCRYGG 1 cut(s) 293
BstENI CCTNNNNNAGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 424, 457
BstKTI GATC 3 cut(s) 23, 165, 548
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 3 cut(s) 20, 162, 545
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstNI CCWGG 1 cut(s) 96
BstNSI RCATGY 2 cut(s) 476, 649
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 213
BsuRI GGCC 2 cut(s) 126, 466
BtgI CCRYGG 1 cut(s) 293
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 2 cut(s) 424, 457
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 460
Cac8I GCNNGC 1 cut(s) 651
CaiI CAGNNNCTG 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 124, 328
Csp6I GTAC 2 cut(s) 355, 536
CviAII CATG 7 cut(s) 112, 155, 374, 473, 492, 556, 646
CviJI RGCY 6 cut(s) 94, 126, 223, 242, 298, 466
CviKI_1 RGCY 6 cut(s) 94, 126, 223, 242, 298, 466
CviQI GTAC 2 cut(s) 355, 536
DdeI CTNAG 2 cut(s) 415, 456
DpnI GATC 3 cut(s) 22, 164, 547
DpnII GATC 3 cut(s) 20, 162, 545
EaeI YGGCCR 1 cut(s) 464
Eco31I GGTCTC 1 cut(s) 557
Eco32I GATATC 2 cut(s) 57, 258
Eco47I GGWCC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 94
EcoRV GATATC 2 cut(s) 57, 258
FaeI CATG 7 cut(s) 115, 158, 377, 476, 495, 559, 649
FaqI GGGAC 2 cut(s) 314, 614
FatI CATG 7 cut(s) 111, 154, 373, 472, 491, 555, 645
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 411, 464
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 2 cut(s) 126, 466
Hin1II CATG 7 cut(s) 115, 158, 377, 476, 495, 559, 649
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 1 cut(s) 27
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 325, 485, 508
Hpy188III TCNNGA 5 cut(s) 24, 197, 360, 428, 525
Hpy8I GTNNAC 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 9
HpyCH4III ACNGT 2 cut(s) 294, 540
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 4 cut(s) 75, 373, 589, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 2 cut(s) 415, 456
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 7 cut(s) 115, 158, 377, 476, 495, 559, 649
Kzo9I GATC 3 cut(s) 20, 162, 545
LmnI GCTCC 2 cut(s) 239, 499
LweI GCATC 2 cut(s) 235, 442
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 379, 517
MalI GATC 3 cut(s) 22, 164, 547
MboI GATC 3 cut(s) 20, 162, 545
MboII GAAGA 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 466
MluCI AATT 2 cut(s) 66, 443
MluNI TGGCCA 1 cut(s) 466
MnlI CCTC 8 cut(s) 57, 193, 319, 345, 414, 490, 615, 650
Mox20I TGGCCA 1 cut(s) 466
MscI TGGCCA 1 cut(s) 466
MseI TTAA 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 123
NdeII GATC 3 cut(s) 20, 162, 545
NlaIII CATG 7 cut(s) 115, 158, 377, 476, 495, 559, 649
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 2 cut(s) 379, 517
NspI RCATGY 2 cut(s) 476, 649
PfeI GAWTC 1 cut(s) 27
PkrI GCNGC 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 328
PsiI TTATAA 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 124, 328
PspPPI RGGWCCY 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 242
RsaI GTAC 2 cut(s) 356, 537
RsaNI GTAC 2 cut(s) 355, 536
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 3 cut(s) 20, 162, 545
Sau96I GGNCC 2 cut(s) 124, 328
ScrFI CCNGG 1 cut(s) 96
SetI ASST 9 cut(s) 204, 244, 330, 356, 519, 553, 617, 661, 672
SfaNI GCATC 2 cut(s) 235, 442
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 328
Sse9I AATT 2 cut(s) 66, 443
SsiI CCGC 1 cut(s) 84
SspI AATATT 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 294, 540
TaiI ACGT 1 cut(s) 519
TaqI TCGA 3 cut(s) 23, 169, 575
TaqII GACCGA 1 cut(s) 155
TasI AATT 2 cut(s) 66, 443
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 2 cut(s) 388, 467
TseFI GTSAC 2 cut(s) 379, 517
Tsp45I GTSAC 2 cut(s) 379, 517
TspDTI ATGAA 1 cut(s) 155
TspRI CASTG 2 cut(s) 388, 467
VpaK11BI GGWCC 1 cut(s) 328
XagI CCTNNNNNAGG 1 cut(s) 654
XceI RCATGY 2 cut(s) 476, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.