MD04G1178200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014985
Physical Location & Seq
Reverse (-)
26918704 .. 26921608
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1178200.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGCGTACAGGAGAAAGCAGGGGATAACAAGGTCGTCAACCTTCAAAGAAGAGATCATTCATCGCCCCCCAGACGACAATTCTGCACCGAATTCATCTTCCCTTCTGCAGTCCTCGTCTTCGTTCTCCGGTTCTTCATCTTCGTCTCTCGCCGCTCAGGCCATTCGTGCTTCTGCGCTTTCCGTCGAACGCAACAACCGATCCAAGGACTTCTCTGCCTATGACGATGCATCTGCAAAATCTGGGTTTTGGGGTGTTCTGGCACGGAAAGCTAAGGCCATTCTTGAAGAGGATGGTGAATTTCCACAAGATGATATTGCTGGAGGGCTCAGATCAAAGCCCTCCGTTGCCTCCGGTGGCGCTCAGTTCCAACAACAAACATATTATCAGTCATATGACAACTCTAGAAGAACGGAAAATCCTACAATCCGAAAGGGCTTGGATAAAATCACGACTTCCCTTAATCAGCTCGGGGACACGTTTGAGAAGGCTTTCGAGGAAGGTCGAACGATGGTGGAGAATAAGACCGCCGACATCCAAAAGCAAATTAAACGAAGAGGAGACAATCCAGATTCACAAAATCAAGCTTCTTATTATGGGAACCAGAACAGTTCACCGCTGCAGCCTAAGAACCATGATACTCAACTCAAGGCATCACGCGACGTTGCAATGGCAACAGCTGCCAAAGCAAAATTACTTCTTCGGGAGCTGAAAACCGTCAAGGCAGATCTGGCTTTTGCAAAGGAACGATGTTCTCAACTAGAAGAAGAAAATAAATGCCTTCGCGAGAATCGTGACAAGGGGAACCACAGAGCTGATGATGACTTGATCCGCCTTCAACTGGAGACACTTCTGGCGGAGAAGGCTCGATTAGCGAATGAGAATTCAGTTTATGCACGCGAGAATCGGTTCCTGAGGGAAATTGTTGAATACCACCAGCTGACAATGCAGGATGTTGTGTATTTAGAAGATGATGAAGAAGTCACACAACTTAACCCCTTAACCAGCCCCACCGGCGTCTCCAGGATGCTCTCCATTTCCCCGCCCTCATCAGCACCCCCATCCCCGCCTCCAGAGGACTCTTCATCTGCAACCTCACCAGCTACTAAAGATATATCACCTGTCCTTACCAAGCCACAAGAAAATAAAGACGTTTTTGTGAATCTGGCCATGCCGGGCAACGATACCTTAGCTCACAAGAAAGCAGATGGTAAAACACCTTCTTCAACAGAAGCTACTACAAAAACACCTCCTACAGCAGAAAGTATAACTAAATCGCCGACAACAGCAGAAGCCGCTACTAAAACACTCTCAGTAGCAGCAGAAGATACTAAAAGACCTCCTCCTACTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

453

Amino Acids

49.47

Weight (kDa)

6.59

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30050
fragaria_vesca FvH4_6g09370
malus_domestica MD04G1178200.v1.1 MD12G1193800.v1.1
prunus_persica Prupe.6G298200_v2.0.a1
pyrus_communis pycom04g15720 pycom12g18000
rosa_chinensis RchiOBHm_Chr3g0459761
rosa_laevigata RLG00000025059
rosa_multiflora Rmu_sc0000240.1_g000037
rosa_roxburghii Rroxscaffold_6G00419980
rosa_rugosa Rorug03G0038100
rosa_samantha Rh3AG096700 Rh3BG099400 Rh3CG101300 Rh3DG100700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 155
AccII CGCG 3 cut(s) 660, 786, 900
AciI CCGC 8 cut(s) 153, 528, 617, 832, 857, 1043, 1067, 1296
AclWI GGATC 2 cut(s) 195, 823
AcoI YGGCCR 1 cut(s) 1167
AcsI RAATTY 3 cut(s) 91, 299, 883
AcyI GRCGYC 1 cut(s) 1017
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 205, 841
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 5 cut(s) 46, 287, 839, 929, 1227
AjnI CCWGG 1 cut(s) 1022
AloI GAACNNNNNNTCC 2 cut(s) 403, 435
Alw26I GTCTC 4 cut(s) 150, 555, 839, 1024
AlwI GGATC 2 cut(s) 195, 823
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 3 cut(s) 159, 276, 1167
ApeKI GCWGC 4 cut(s) 619, 622, 680, 1319
ApoI RAATTY 3 cut(s) 91, 299, 883
Asp700I GAANNNNTTC 2 cut(s) 491, 908
AspLEI GCGC 2 cut(s) 178, 362
AsuC2I CCSGG 1 cut(s) 1176
AsuHPI GGTGA 4 cut(s) 308, 606, 1089, 1110
AvaI CYCGRG 1 cut(s) 470
AxyI CCTNAGG 1 cut(s) 914
BalI TGGCCA 1 cut(s) 1169
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 1204, 1236
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 606, 634, 667, 1331
BccI CCATC 4 cut(s) 287, 505, 1069, 1202
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 215, 249
BciT130I CCWGG 1 cut(s) 1024
BcnI CCSGG 1 cut(s) 1176
BcoDI GTCTC 4 cut(s) 150, 555, 839, 1024
BfaI CTAG 2 cut(s) 405, 761
BfmI CTRYAG 3 cut(s) 107, 620, 1254
BfoI RGCGCY 1 cut(s) 363
BglI GCCNNNNNGGC 2 cut(s) 158, 1014
BglII AGATCT 1 cut(s) 727
BisI GCNGC 6 cut(s) 153, 620, 623, 681, 1296, 1320
BlsI GCNGC 6 cut(s) 154, 621, 624, 682, 1297, 1321
Bme1390I CCNGG 2 cut(s) 1024, 1176
BmeT110I CYCGRG 1 cut(s) 470
BmiI GGNNCC 3 cut(s) 602, 806, 911
BmrFI CCNGG 2 cut(s) 1024, 1176
BmsI GCATC 4 cut(s) 217, 239, 662, 1017
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 4 cut(s) 342, 863, 1006, 1056
Bpu10I CCTNAGC 3 cut(s) 156, 273, 1189
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 204
BsaWI WCCGGW 2 cut(s) 128, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 841
Bse118I RCCGGY 1 cut(s) 1013
Bse1I ACTGG 1 cut(s) 846
Bse21I CCTNAGG 1 cut(s) 914
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 1 cut(s) 1024
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 5 cut(s) 298, 534, 958, 1032, 1061
BseLI CCNNNNNNNGG 2 cut(s) 205, 841
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 5 cut(s) 170, 343, 377, 905, 1326
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 2 cut(s) 573, 1332
BseXI GCAGC 4 cut(s) 606, 634, 667, 1331
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 3 cut(s) 660, 786, 900
BshFI GGCC 3 cut(s) 161, 278, 1169
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 4 cut(s) 129, 354, 1014, 1175
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 2 cut(s) 205, 841
BsmAI GTCTC 4 cut(s) 150, 555, 839, 1024
BsmBI CGTCTC 2 cut(s) 150, 1024
BsmFI GGGAC 1 cut(s) 488
BsnI GGCC 3 cut(s) 161, 278, 1169
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 5 cut(s) 54, 200, 332, 727, 828
Bsp68I TCGCGA 1 cut(s) 786
BspACI CCGC 8 cut(s) 153, 528, 617, 832, 857, 1043, 1067, 1296
BspANI GGCC 3 cut(s) 161, 278, 1169
BspCNI CTCAG 5 cut(s) 169, 342, 376, 906, 1325
BspFNI CGCG 3 cut(s) 660, 786, 900
BspLI GGNNCC 3 cut(s) 602, 806, 911
BspMAI CTGCAG 2 cut(s) 111, 624
BspPI GGATC 2 cut(s) 195, 823
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 675
BsrFI RCCGGY 1 cut(s) 1013
BsrI ACTGG 1 cut(s) 846
BssAI RCCGGY 1 cut(s) 1013
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 5 cut(s) 54, 200, 332, 727, 828
BssNI GRCGYC 1 cut(s) 1017
BssT1I CCWWGG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 1024
Bst4CI ACNGT 2 cut(s) 611, 718
Bst6I CTCTTC 4 cut(s) 45, 282, 550, 1087
BstACI GRCGYC 1 cut(s) 1017
BstAPI GCANNNNNTGC 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 898
BstDEI CTNAG 8 cut(s) 156, 273, 329, 363, 627, 914, 1189, 1312
BstF5I GGATG 5 cut(s) 298, 534, 958, 1032, 1061
BstFNI CGCG 3 cut(s) 660, 786, 900
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 2 cut(s) 178, 362
BstKTI GATC 5 cut(s) 57, 203, 335, 730, 831
BstMAI GTCTC 4 cut(s) 150, 555, 839, 1024
BstMBI GATC 5 cut(s) 54, 200, 332, 727, 828
BstNI CCWGG 1 cut(s) 1024
BstSCI CCNGG 2 cut(s) 1022, 1174
BstSFI CTRYAG 3 cut(s) 107, 620, 1254
BstUI CGCG 3 cut(s) 660, 786, 900
BstV1I GCAGC 4 cut(s) 606, 634, 667, 1331
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
Bsu36I CCTNAGG 1 cut(s) 914
BsuRI GGCC 3 cut(s) 161, 278, 1169
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 5 cut(s) 298, 534, 958, 1032, 1061
BtuMI TCGCGA 1 cut(s) 786
Cac8I GCNNGC 1 cut(s) 898
CfoI GCGC 2 cut(s) 178, 362
Cfr10I RCCGGY 1 cut(s) 1013
CseI GACGC 1 cut(s) 1006
Csp6I GTAC 1 cut(s) 7
CviAII CATG 2 cut(s) 635, 1171
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 8 cut(s) 156, 273, 329, 363, 627, 914, 1189, 1312
DpnI GATC 5 cut(s) 56, 202, 334, 729, 830
DpnII GATC 5 cut(s) 54, 200, 332, 727, 828
EaeI YGGCCR 1 cut(s) 1167
Eam1104I CTCTTC 4 cut(s) 45, 282, 550, 1087
EarI CTCTTC 4 cut(s) 45, 282, 550, 1087
EciI GGCGGA 2 cut(s) 821, 872
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 914
Eco88I CYCGRG 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 91, 883
EcoRII CCWGG 1 cut(s) 1022
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 2 cut(s) 150, 1024
FaeI CATG 2 cut(s) 638, 1174
FaqI GGGAC 1 cut(s) 488
FatI CATG 2 cut(s) 634, 1170
FauI CCCGC 2 cut(s) 1050, 1074
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 6 cut(s) 153, 620, 623, 681, 1296, 1320
FokI GGATG 5 cut(s) 305, 521, 965, 1039, 1048
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 6 cut(s) 153, 620, 623, 681, 1296, 1320
FspBI CTAG 2 cut(s) 405, 761
GlaI GCGC 2 cut(s) 177, 361
GluI GCNGC 6 cut(s) 153, 620, 623, 681, 1296, 1320
GsuI CTGGAG 4 cut(s) 342, 863, 1006, 1056
HaeII RGCGCY 1 cut(s) 363
HaeIII GGCC 3 cut(s) 161, 278, 1169
HapII CCGG 4 cut(s) 129, 354, 1014, 1175
HgaI GACGC 1 cut(s) 1006
HhaI GCGC 2 cut(s) 178, 362
Hin1I GRCGYC 1 cut(s) 1017
Hin1II CATG 2 cut(s) 638, 1174
Hin6I GCGC 2 cut(s) 176, 360
HinP1I GCGC 2 cut(s) 176, 360
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 5 cut(s) 572, 790, 904, 1079, 1162
HpaII CCGG 4 cut(s) 129, 354, 1014, 1175
HphI GGTGA 4 cut(s) 308, 606, 1089, 1110
Hpy166II GTNNAC 2 cut(s) 39, 614
Hpy188I TCNGA 2 cut(s) 332, 431
Hpy188III TCNNGA 9 cut(s) 284, 405, 451, 569, 704, 785, 794, 913, 1073
Hpy8I GTNNAC 2 cut(s) 39, 614
Hpy99I CGWCG 2 cut(s) 188, 665
HpyAV CCTTC 8 cut(s) 52, 113, 481, 494, 791, 845, 856, 1230
HpyCH4III ACNGT 2 cut(s) 611, 718
HpyCH4IV ACGT 3 cut(s) 479, 663, 1152
HpyF3I CTNAG 8 cut(s) 156, 273, 329, 363, 627, 914, 1189, 1312
HpySE526I ACGT 3 cut(s) 479, 663, 1152
Hsp92I GRCGYC 1 cut(s) 1017
Hsp92II CATG 2 cut(s) 638, 1174
HspAI GCGC 2 cut(s) 176, 360
Kzo9I GATC 5 cut(s) 54, 200, 332, 727, 828
LmnI GCTCC 1 cut(s) 706
Lsp1109I GCAGC 4 cut(s) 606, 634, 667, 1331
LweI GCATC 4 cut(s) 217, 239, 662, 1017
MaeI CTAG 2 cut(s) 405, 761
MaeII ACGT 3 cut(s) 479, 663, 1152
MaeIII GTNAC 2 cut(s) 794, 982
MalI GATC 5 cut(s) 56, 202, 334, 729, 830
MbiI CCGCTC 1 cut(s) 155
MboI GATC 5 cut(s) 54, 200, 332, 727, 828
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 1169
MluCI AATT 7 cut(s) 79, 91, 299, 546, 692, 883, 921
MluNI TGGCCA 1 cut(s) 1169
MlyI GAGTC 1 cut(s) 1073
MmeI TCCRAC 1 cut(s) 394
Mox20I TGGCCA 1 cut(s) 1169
Mph1103I ATGCAT 1 cut(s) 232
MroXI GAANNNNTTC 2 cut(s) 491, 908
MscI TGGCCA 1 cut(s) 1169
MseI TTAA 5 cut(s) 462, 549, 993, 1001, 1357
Msp20I TGGCCA 1 cut(s) 1169
MspA1I CMGCKG 3 cut(s) 619, 680, 940
MspI CCGG 4 cut(s) 129, 354, 1014, 1175
MspR9I CCNGG 2 cut(s) 1024, 1176
MvaI CCWGG 1 cut(s) 1024
MvnI CGCG 3 cut(s) 660, 786, 900
NciI CCSGG 1 cut(s) 1176
NdeI CATATG 1 cut(s) 394
NdeII GATC 5 cut(s) 54, 200, 332, 727, 828
NlaIII CATG 2 cut(s) 638, 1174
NlaIV GGNNCC 3 cut(s) 602, 806, 911
NmuCI GTSAC 2 cut(s) 794, 982
NruI TCGCGA 1 cut(s) 786
NsiI ATGCAT 1 cut(s) 232
PcsI WCGNNNNNNNCGW 2 cut(s) 196, 790
PdmI GAANNNNTTC 2 cut(s) 491, 908
PfeI GAWTC 4 cut(s) 572, 790, 904, 1162
PfoI TCCNGGA 1 cut(s) 1022
PkrI GCNGC 6 cut(s) 154, 621, 624, 682, 1297, 1321
PleI GAGTC 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1073
Psp6I CCWGG 1 cut(s) 1022
PspGI CCWGG 1 cut(s) 1022
PspN4I GGNNCC 3 cut(s) 602, 806, 911
PstI CTGCAG 2 cut(s) 111, 624
PsuI RGATCY 1 cut(s) 727
PvuII CAGCTG 2 cut(s) 680, 940
RruI TCGCGA 1 cut(s) 786
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 5 cut(s) 462, 549, 993, 1001, 1357
SatI GCNGC 6 cut(s) 153, 620, 623, 681, 1296, 1320
Sau3AI GATC 5 cut(s) 54, 200, 332, 727, 828
SchI GAGTC 1 cut(s) 1073
ScrFI CCNGG 2 cut(s) 1024, 1176
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 4 cut(s) 217, 239, 662, 1017
SfcI CTRYAG 3 cut(s) 107, 620, 1254
SgrAI CRCCGGYG 1 cut(s) 1013
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
Sse9I AATT 7 cut(s) 79, 91, 299, 546, 692, 883, 921
SsiI CCGC 8 cut(s) 153, 528, 617, 832, 857, 1043, 1067, 1296
SspMI CTAG 2 cut(s) 405, 761
StyD4I CCNGG 2 cut(s) 1022, 1174
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 611, 718
TaiI ACGT 3 cut(s) 482, 666, 1155
TaqI TCGA 4 cut(s) 186, 495, 505, 868
TasI AATT 7 cut(s) 79, 91, 299, 546, 692, 883, 921
TauI GCSGC 2 cut(s) 155, 1298
TfiI GAWTC 4 cut(s) 572, 790, 904, 1162
Tru1I TTAA 5 cut(s) 462, 549, 993, 1001, 1357
Tru9I TTAA 5 cut(s) 462, 549, 993, 1001, 1357
TseFI GTSAC 2 cut(s) 794, 982
TseI GCWGC 4 cut(s) 619, 622, 680, 1319
Tsp45I GTSAC 2 cut(s) 794, 982
TspDTI ATGAA 5 cut(s) 50, 84, 126, 990, 1074
TspGWI ACGGA 4 cut(s) 172, 280, 334, 428
XapI RAATTY 3 cut(s) 91, 299, 883
XbaI TCTAGA 1 cut(s) 404
XmnI GAANNNNTTC 2 cut(s) 491, 908
XspI CTAG 2 cut(s) 405, 761
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.