MD12G1193800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014985
Physical Location & Seq
Reverse (-)
27529287 .. 27531909
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1193800.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGCGTACAGGAGAAGGCAGGGGATAACAAGGTCGTCAACCTTCAAAGAAGAGATCATTCATCGCCCGCCGGACAACAGTTCTTCACCGAACTCATCTTCCCCTCTGCAGTCTTCATCGTCCTTCTCCGGTTCTTCGTCTCTCGCGGCTCAGGCGATCCGTGCTTCTGCGCTTTCCGTTGAACGCAAAAACCGATCCCAGGACTTCTCTGCCTATGACGACGCATCCGCAAAATCTGGGTTTTGGGGGGTTCTGGCTAGGAAAGCGAAGGCCATTCTCGAAGAGGATGGTGAATTCCCACAAGATGATGCGGCTGGAGGACTCGTATCGCAGCCCTCTATTGCCTCCGGTGGCACTCAGCTCCAACAACAACCATATTATCAGTCATATGACAACTCTAGAAGAACGGAAAATCCTGCACTCCGAAAGGGCTTGGATAGAATCACAACTTCCCTTAATCAGCTCGGGGACACGTTTGAGAAAGCTTTCGAGGAAGGTCGAACGATAGTGGAGAATAAGACAGCCGACATCAAAATGCAAATTAAACGAAGGGGAGACAATCCTGATGCGCATGATCAAGCTTCTTATTATGGGAACCAGAACAGTTCACCGTTGCAGCCTAAGAACCACGATACTCAACTCAAGGCATCACGCGACGTTGCAATGGCAACGGCTGCCAAAGCAAAATTACTTCTTCGGGAGCTGAAAACCGTTAAGGCAGATCTGGCTTTTGCGAAGGAACGATGTTCTCAACTAGAAGAAGAAAATAAACGCCTTCGCGAGAATCGTGACAAGGGAAACCACAGAGCAGATGATGACTTGATCCGCCTTCAACTGGAGACACTTCTGGCAGAGAAGGCTCGATTAGCGAACGAGAATTCAGTTTATGCACGCGAGAATCGGTTCCTTAGGGAAATTGTTGAATACCACCAGCTGACAATGCAGGATGTTGTGTATATGGATGATGAAGAAGTCACAGAAGTTAACCCCTTAACCAGCCCCACCGGCGTCTCCAGGATGCTCTCCATTTCCCCGCCTTCATCAGCACCCCCATCCCCGCCTCCAGAGGACTCTTCATCTGCAACCTCACCAGTTACTAAAGAAATATCTCCTATCCTCACCAAGCCACAAGAAAATAAAGACGTTTTTGCGAATGAGGCCATGCCAAGCTTCGATACCTTAGTTCCCAAGCAAGAACATGCTAAAACACCTTCTTCAGCAGGAGCTGCTACCAAAACACATTCTTCAACAGCAGAAGCCGCAACTAAAACACTCTCCGTAGCAGCAGAAGATTCTAAAAGACCTCCTGCTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

440

Amino Acids

48.14

Weight (kDa)

6.25

Isoelectric Point (pI)

68.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30050
fragaria_vesca FvH4_6g09370
malus_domestica MD04G1178200.v1.1 MD12G1193800.v1.1
prunus_persica Prupe.6G298200_v2.0.a1
pyrus_communis pycom04g15720 pycom12g18000
rosa_chinensis RchiOBHm_Chr3g0459761
rosa_laevigata RLG00000025059
rosa_multiflora Rmu_sc0000240.1_g000037
rosa_roxburghii Rroxscaffold_6G00419980
rosa_rugosa Rorug03G0038100
rosa_samantha Rh3AG096700 Rh3BG099400 Rh3CG101300 Rh3DG100700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 570
AccII CGCG 4 cut(s) 146, 654, 780, 894
AciI CCGC 8 cut(s) 68, 146, 228, 311, 826, 1034, 1058, 1260
AclWI GGATC 3 cut(s) 151, 189, 817
AcsI RAATTY 2 cut(s) 293, 877
AcuI CTGAAG 1 cut(s) 1200
AcyI GRCGYC 1 cut(s) 1008
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 199, 835
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 5 cut(s) 46, 182, 833, 923, 1248
AjnI CCWGG 2 cut(s) 198, 1013
AloI GAACNNNNNNTCC 4 cut(s) 83, 115, 397, 429
AluBI AGCT 8 cut(s) 361, 463, 485, 581, 703, 934, 1170, 1226
AluI AGCT 8 cut(s) 361, 463, 485, 581, 703, 934, 1170, 1226
Alw26I GTCTC 4 cut(s) 144, 549, 833, 1015
AlwI GGATC 3 cut(s) 151, 189, 817
AlwNI CAGNNNCTG 1 cut(s) 1226
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 2 cut(s) 270, 1158
ApeKI GCWGC 5 cut(s) 331, 616, 674, 1226, 1283
ApoI RAATTY 2 cut(s) 293, 877
Asp700I GAANNNNTTC 2 cut(s) 485, 902
AspLEI GCGC 2 cut(s) 172, 571
AsuHPI GGTGA 5 cut(s) 78, 302, 600, 1080, 1111
AvaI CYCGRG 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 908
BaeI ACNNNNGTAYC 2 cut(s) 1167, 1200
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 5 cut(s) 343, 628, 661, 1213, 1295
BccI CCATC 2 cut(s) 281, 1060
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 2 cut(s) 200, 1015
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 4 cut(s) 144, 549, 833, 1015
BfaI CTAG 3 cut(s) 258, 399, 755
BfmI CTRYAG 1 cut(s) 107
BglI GCCNNNNNGGC 1 cut(s) 1005
BglII AGATCT 1 cut(s) 721
BisI GCNGC 8 cut(s) 147, 312, 332, 617, 675, 1227, 1260, 1284
BlsI GCNGC 8 cut(s) 148, 313, 333, 618, 676, 1228, 1261, 1285
Bme1390I CCNGG 2 cut(s) 200, 1015
BmeT110I CYCGRG 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 596, 905
BmrFI CCNGG 2 cut(s) 200, 1015
BmsI GCATC 5 cut(s) 233, 298, 556, 656, 1008
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 4 cut(s) 336, 857, 997, 1047
Bpu10I CCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 1008
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 128, 347
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 835
Bse118I RCCGGY 1 cut(s) 1004
Bse1I ACTGG 2 cut(s) 840, 1091
Bse21I CCTNAGG 1 cut(s) 908
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 200, 1015
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 6 cut(s) 224, 292, 952, 967, 1023, 1052
BseLI CCNNNNNNNGG 2 cut(s) 199, 835
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 164, 371
BseNI ACTGG 2 cut(s) 840, 1091
BseXI GCAGC 5 cut(s) 343, 628, 661, 1213, 1295
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 4 cut(s) 146, 654, 780, 894
BshFI GGCC 2 cut(s) 272, 1160
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 4 cut(s) 71, 129, 348, 1005
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 2 cut(s) 199, 835
BsmAI GTCTC 4 cut(s) 144, 549, 833, 1015
BsmBI CGTCTC 2 cut(s) 144, 1015
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 2 cut(s) 272, 1160
BsoBI CYCGRG 1 cut(s) 464
Bsp143I GATC 6 cut(s) 54, 156, 194, 574, 721, 822
Bsp68I TCGCGA 1 cut(s) 780
BspACI CCGC 8 cut(s) 68, 146, 228, 311, 826, 1034, 1058, 1260
BspANI GGCC 2 cut(s) 272, 1160
BspCNI CTCAG 2 cut(s) 163, 370
BspFNI CGCG 4 cut(s) 146, 654, 780, 894
BspLI GGNNCC 2 cut(s) 596, 905
BspMAI CTGCAG 1 cut(s) 111
BspPI GGATC 3 cut(s) 151, 189, 817
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1004
BsrI ACTGG 2 cut(s) 840, 1091
BssAI RCCGGY 1 cut(s) 1004
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 6 cut(s) 54, 156, 194, 574, 721, 822
BssNI GRCGYC 1 cut(s) 1008
Bst2UI CCWGG 2 cut(s) 200, 1015
Bst4CI ACNGT 4 cut(s) 80, 605, 612, 712
Bst6I CTCTTC 3 cut(s) 45, 276, 1078
BstACI GRCGYC 1 cut(s) 1008
BstAPI GCANNNNNTGC 2 cut(s) 674, 1226
BstC8I GCNNGC 2 cut(s) 68, 892
BstDEI CTNAG 5 cut(s) 150, 357, 621, 908, 1180
BstF5I GGATG 6 cut(s) 224, 292, 952, 967, 1023, 1052
BstFNI CGCG 4 cut(s) 146, 654, 780, 894
BstHHI GCGC 2 cut(s) 172, 571
BstKTI GATC 6 cut(s) 57, 159, 197, 577, 724, 825
BstMAI GTCTC 4 cut(s) 144, 549, 833, 1015
BstMBI GATC 6 cut(s) 54, 156, 194, 574, 721, 822
BstNI CCWGG 2 cut(s) 200, 1015
BstNSI RCATGY 1 cut(s) 1202
BstSCI CCNGG 2 cut(s) 198, 1013
BstSFI CTRYAG 1 cut(s) 107
BstUI CGCG 4 cut(s) 146, 654, 780, 894
BstV1I GCAGC 5 cut(s) 343, 628, 661, 1213, 1295
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
Bsu36I CCTNAGG 1 cut(s) 908
BsuRI GGCC 2 cut(s) 272, 1160
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 6 cut(s) 224, 292, 952, 967, 1023, 1052
BtuMI TCGCGA 1 cut(s) 780
Cac8I GCNNGC 2 cut(s) 68, 892
CaiI CAGNNNCTG 1 cut(s) 1226
CfoI GCGC 2 cut(s) 172, 571
Cfr10I RCCGGY 1 cut(s) 1004
CseI GACGC 2 cut(s) 230, 997
Csp6I GTAC 1 cut(s) 7
CviAII CATG 3 cut(s) 572, 1162, 1199
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 5 cut(s) 150, 357, 621, 908, 1180
DpnI GATC 6 cut(s) 56, 158, 196, 576, 723, 824
DpnII GATC 6 cut(s) 54, 156, 194, 574, 721, 822
Eam1104I CTCTTC 3 cut(s) 45, 276, 1078
EarI CTCTTC 3 cut(s) 45, 276, 1078
EciI GGCGGA 1 cut(s) 815
Eco57I CTGAAG 1 cut(s) 1200
Eco81I CCTNAGG 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 464
EcoRI GAATTC 2 cut(s) 293, 877
EcoRII CCWGG 2 cut(s) 198, 1013
Esp3I CGTCTC 2 cut(s) 144, 1015
FaeI CATG 3 cut(s) 575, 1165, 1202
FaqI GGGAC 1 cut(s) 482
FatI CATG 3 cut(s) 571, 1161, 1198
FauI CCCGC 3 cut(s) 75, 1041, 1065
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 8 cut(s) 147, 312, 332, 617, 675, 1227, 1260, 1284
FokI GGATG 6 cut(s) 211, 299, 959, 974, 1030, 1039
Fsp4HI GCNGC 8 cut(s) 147, 312, 332, 617, 675, 1227, 1260, 1284
FspAI RTGCGCAY 1 cut(s) 570
FspBI CTAG 3 cut(s) 258, 399, 755
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 2 cut(s) 171, 570
GluI GCNGC 8 cut(s) 147, 312, 332, 617, 675, 1227, 1260, 1284
GsuI CTGGAG 4 cut(s) 336, 857, 997, 1047
HaeIII GGCC 2 cut(s) 272, 1160
HapII CCGG 4 cut(s) 71, 129, 348, 1005
HgaI GACGC 2 cut(s) 230, 997
HhaI GCGC 2 cut(s) 172, 571
Hin1I GRCGYC 1 cut(s) 1008
Hin1II CATG 3 cut(s) 575, 1165, 1202
Hin6I GCGC 2 cut(s) 170, 569
HinP1I GCGC 2 cut(s) 170, 569
HincII GTYRAC 2 cut(s) 39, 985
HindII GTYRAC 2 cut(s) 39, 985
HindIII AAGCTT 3 cut(s) 483, 579, 1168
HinfI GANTC 6 cut(s) 321, 441, 784, 898, 1070, 1292
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 4 cut(s) 71, 129, 348, 1005
HphI GGTGA 5 cut(s) 78, 302, 600, 1080, 1111
Hpy166II GTNNAC 3 cut(s) 39, 608, 985
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 7 cut(s) 278, 399, 563, 698, 779, 788, 1064
Hpy8I GTNNAC 3 cut(s) 39, 608, 985
Hpy99I CGWCG 2 cut(s) 224, 659
HpyCH4III ACNGT 4 cut(s) 80, 605, 612, 712
HpyCH4IV ACGT 3 cut(s) 473, 657, 1143
HpyCH4V TGCA 8 cut(s) 109, 419, 538, 616, 662, 890, 943, 1082
HpyF3I CTNAG 5 cut(s) 150, 357, 621, 908, 1180
HpySE526I ACGT 3 cut(s) 473, 657, 1143
Hsp92I GRCGYC 1 cut(s) 1008
Hsp92II CATG 3 cut(s) 575, 1165, 1202
HspAI GCGC 2 cut(s) 170, 569
Ksp22I TGATCA 1 cut(s) 574
KspAI GTTAAC 1 cut(s) 985
Kzo9I GATC 6 cut(s) 54, 156, 194, 574, 721, 822
LmnI GCTCC 3 cut(s) 366, 700, 1223
Lsp1109I GCAGC 5 cut(s) 343, 628, 661, 1213, 1295
LweI GCATC 5 cut(s) 233, 298, 556, 656, 1008
MaeI CTAG 3 cut(s) 258, 399, 755
MaeII ACGT 3 cut(s) 473, 657, 1143
MaeIII GTNAC 3 cut(s) 788, 973, 1093
MalI GATC 6 cut(s) 56, 158, 196, 576, 723, 824
MboI GATC 6 cut(s) 54, 156, 194, 574, 721, 822
MflI RGATCY 1 cut(s) 721
MluCI AATT 5 cut(s) 293, 540, 686, 877, 915
MlyI GAGTC 2 cut(s) 315, 1064
MmeI TCCRAC 1 cut(s) 388
MroXI GAANNNNTTC 2 cut(s) 485, 902
MseI TTAA 6 cut(s) 456, 543, 714, 984, 992, 1318
MslI CAYNNNNRTG 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 934
MspI CCGG 4 cut(s) 71, 129, 348, 1005
MspR9I CCNGG 2 cut(s) 200, 1015
MvaI CCWGG 2 cut(s) 200, 1015
MvnI CGCG 4 cut(s) 146, 654, 780, 894
NdeI CATATG 1 cut(s) 388
NdeII GATC 6 cut(s) 54, 156, 194, 574, 721, 822
NlaIII CATG 3 cut(s) 575, 1165, 1202
NlaIV GGNNCC 2 cut(s) 596, 905
NmuCI GTSAC 2 cut(s) 788, 973
NruI TCGCGA 1 cut(s) 780
NsbI TGCGCA 1 cut(s) 570
NspI RCATGY 1 cut(s) 1202
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 784
PdmI GAANNNNTTC 2 cut(s) 485, 902
PfeI GAWTC 4 cut(s) 441, 784, 898, 1292
PfoI TCCNGGA 1 cut(s) 1013
PkrI GCNGC 8 cut(s) 148, 313, 333, 618, 676, 1228, 1261, 1285
PleI GAGTC 2 cut(s) 315, 1064
PpsI GAGTC 2 cut(s) 315, 1064
Psp6I CCWGG 2 cut(s) 198, 1013
PspGI CCWGG 2 cut(s) 198, 1013
PspN4I GGNNCC 2 cut(s) 596, 905
PstI CTGCAG 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 1 cut(s) 721
PvuII CAGCTG 1 cut(s) 934
RruI TCGCGA 1 cut(s) 780
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 6 cut(s) 456, 543, 714, 984, 992, 1318
SatI GCNGC 8 cut(s) 147, 312, 332, 617, 675, 1227, 1260, 1284
Sau3AI GATC 6 cut(s) 54, 156, 194, 574, 721, 822
SchI GAGTC 2 cut(s) 315, 1064
ScrFI CCNGG 2 cut(s) 200, 1015
SfaNI GCATC 5 cut(s) 233, 298, 556, 656, 1008
SfcI CTRYAG 1 cut(s) 107
SgrAI CRCCGGYG 1 cut(s) 1004
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 5 cut(s) 293, 540, 686, 877, 915
SsiI CCGC 8 cut(s) 68, 146, 228, 311, 826, 1034, 1058, 1260
SspMI CTAG 3 cut(s) 258, 399, 755
StyD4I CCNGG 2 cut(s) 198, 1013
TaaI ACNGT 4 cut(s) 80, 605, 612, 712
TaiI ACGT 3 cut(s) 476, 660, 1146
TaqI TCGA 5 cut(s) 279, 489, 499, 862, 1173
TasI AATT 5 cut(s) 293, 540, 686, 877, 915
TauI GCSGC 3 cut(s) 149, 314, 1262
TfiI GAWTC 4 cut(s) 441, 784, 898, 1292
Tru1I TTAA 6 cut(s) 456, 543, 714, 984, 992, 1318
Tru9I TTAA 6 cut(s) 456, 543, 714, 984, 992, 1318
TseFI GTSAC 2 cut(s) 788, 973
TseI GCWGC 5 cut(s) 331, 616, 674, 1226, 1283
Tsp45I GTSAC 2 cut(s) 788, 973
TspDTI ATGAA 5 cut(s) 50, 105, 981, 1029, 1065
TspGWI ACGGA 4 cut(s) 149, 166, 422, 1267
XapI RAATTY 2 cut(s) 293, 877
XbaI TCTAGA 1 cut(s) 398
XceI RCATGY 1 cut(s) 1202
XmnI GAANNNNTTC 2 cut(s) 485, 902
XspI CTAG 3 cut(s) 258, 399, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.