pycom04g15720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014985
Physical Location & Seq
Reverse (-)
18523548 .. 18524397
850 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15720.1

Sequence Viewer

Length: 627 bp
ATGGCTTTTGCGAAGGAACGATGTTCTCAACTTGAAGAAGAAAATAAACGCCTTCGCGAGAATCGTGACAAGGGGAACCACAGAGCTGATGACGACTTGATCCGCCTTCAACTGGAGACACTTCTGGCGGAGAAGGCTCGATTAGCAAACGAGAATTCAGTTTATGCACGCGAGAATCGATTCCTGAGGGAAATTGTTGAATACCACCAGCTGACAATGCAGGATGTTGTGTATTTAGAAGATGATGAAGAAGTCACAGAAGTTAACCCCTTAACCAGCCCCACCGGCGTCTCCAGGATGCTCTCCATTTCCCCGCCCTCATCAGCACCCCCATCCCCGCCTCCAGAGGACTCTTCATCAGCAACCTCACCAGCTACTAAAGAAATACCTGTCCTTACCAAGCCACAAGAAAATAAAGACGTTTTTGCAAGTCAGGCCATGCCAGGCAACGATACCTTAGCTCACAAGAAAGAAGATGCTAGAACACCTTCTTCAACAGAAGCTACTGCAAAAACACCTCCTACAGCAGATGGCATCACTAAATCGCTGACAGCAGCAGAAGCCGGTACTAAAACACTCTCTGTAGCAGCAGAAGATACTAAAAGACCTCCTCCTGCTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.71

Weight (kDa)

4.99

Isoelectric Point (pI)

61.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30050
fragaria_vesca FvH4_6g09370
malus_domestica MD04G1178200.v1.1 MD12G1193800.v1.1
prunus_persica Prupe.6G298200_v2.0.a1
pyrus_communis pycom04g15720 pycom12g18000
rosa_chinensis RchiOBHm_Chr3g0459761
rosa_laevigata RLG00000025059
rosa_multiflora Rmu_sc0000240.1_g000037
rosa_roxburghii Rroxscaffold_6G00419980
rosa_rugosa Rorug03G0038100
rosa_samantha Rh3AG096700 Rh3BG099400 Rh3CG101300 Rh3DG100700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 57, 171
AciI CCGC 4 cut(s) 103, 128, 314, 338
AclWI GGATC 1 cut(s) 94
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 4 cut(s) 35, 110, 200, 495
AjnI CCWGG 2 cut(s) 293, 442
AluBI AGCT 5 cut(s) 86, 211, 374, 461, 503
AluI AGCT 5 cut(s) 86, 211, 374, 461, 503
Alw26I GTCTC 2 cut(s) 110, 295
AlwI GGATC 1 cut(s) 94
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 554, 587
ApoI RAATTY 1 cut(s) 154
Asp700I GAANNNNTTC 2 cut(s) 179, 487
AsuHPI GGTGA 1 cut(s) 360
AxyI CCTNAGG 1 cut(s) 185
BbvI GCAGC 2 cut(s) 566, 599
BccI CCATC 2 cut(s) 340, 524
BciT130I CCWGG 2 cut(s) 295, 444
BcoDI GTCTC 2 cut(s) 110, 295
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 2 cut(s) 522, 582
BglI GCCNNNNNGGC 1 cut(s) 285
BisI GCNGC 2 cut(s) 555, 588
BlsI GCNGC 2 cut(s) 556, 589
Bme1390I CCNGG 2 cut(s) 295, 444
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 2 cut(s) 295, 444
BmsI GCATC 3 cut(s) 288, 466, 543
BpmI CTGGAG 3 cut(s) 134, 277, 327
Bpu10I CCTNAGC 1 cut(s) 457
Bsa29I ATCGAT 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 288
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse118I RCCGGY 2 cut(s) 284, 563
Bse1I ACTGG 1 cut(s) 117
Bse21I CCTNAGG 1 cut(s) 185
BseBI CCWGG 2 cut(s) 295, 444
BseCI ATCGAT 1 cut(s) 178
BseGI GGATG 3 cut(s) 229, 303, 332
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 600
BseXI GCAGC 2 cut(s) 566, 599
Bsh1236I CGCG 2 cut(s) 57, 171
BshFI GGCC 1 cut(s) 437
BshVI ATCGAT 1 cut(s) 178
BsiSI CCGG 2 cut(s) 285, 564
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 2 cut(s) 110, 295
BsmBI CGTCTC 1 cut(s) 295
BsnI GGCC 1 cut(s) 437
Bsp143I GATC 1 cut(s) 99
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 4 cut(s) 103, 128, 314, 338
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 1 cut(s) 177
BspDI ATCGAT 1 cut(s) 178
BspFNI CGCG 2 cut(s) 57, 171
BspLI GGNNCC 1 cut(s) 77
BspPI GGATC 1 cut(s) 94
BsrFI RCCGGY 2 cut(s) 284, 563
BsrI ACTGG 1 cut(s) 117
BssAI RCCGGY 2 cut(s) 284, 563
BssMI GATC 1 cut(s) 99
BssNI GRCGYC 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 295, 444
Bst6I CTCTTC 1 cut(s) 358
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 2 cut(s) 185, 457
BstF5I GGATG 3 cut(s) 229, 303, 332
BstFNI CGCG 2 cut(s) 57, 171
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 2 cut(s) 110, 295
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 6 cut(s) 134, 143, 217, 285, 434, 560
BstNI CCWGG 2 cut(s) 295, 444
BstSCI CCNGG 2 cut(s) 293, 442
BstSFI CTRYAG 2 cut(s) 522, 582
BstUI CGCG 2 cut(s) 57, 171
BstV1I GCAGC 2 cut(s) 566, 599
Bsu15I ATCGAT 1 cut(s) 178
Bsu36I CCTNAGG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 437
BsuTUI ATCGAT 1 cut(s) 178
BtsCI GGATG 3 cut(s) 229, 303, 332
BtuMI TCGCGA 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 169
Cfr10I RCCGGY 2 cut(s) 284, 563
ClaI ATCGAT 1 cut(s) 178
CseI GACGC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 567
CviAII CATG 1 cut(s) 439
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 2 cut(s) 185, 457
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
EciI GGCGGA 2 cut(s) 92, 143
Eco81I CCTNAGG 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 293, 442
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 1 cut(s) 442
FaiI YATR 2 cut(s) 165, 440
FatI CATG 1 cut(s) 438
FauI CCCGC 2 cut(s) 321, 345
Fnu4HI GCNGC 2 cut(s) 555, 588
FokI GGATG 3 cut(s) 236, 310, 319
Fsp4HI GCNGC 2 cut(s) 555, 588
FspBI CTAG 1 cut(s) 480
GluI GCNGC 2 cut(s) 555, 588
GsuI CTGGAG 3 cut(s) 134, 277, 327
HaeIII GGCC 1 cut(s) 437
HapII CCGG 2 cut(s) 285, 564
HgaI GACGC 1 cut(s) 277
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 1 cut(s) 442
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 4 cut(s) 61, 175, 180, 350
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 2 cut(s) 285, 564
HphI GGTGA 1 cut(s) 360
Hpy166II GTNNAC 1 cut(s) 265
Hpy188III TCNNGA 4 cut(s) 56, 65, 184, 344
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 5 cut(s) 7, 62, 116, 127, 498
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 4 cut(s) 167, 220, 428, 509
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 143, 217, 285, 434, 560
HpyF3I CTNAG 2 cut(s) 185, 457
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 1 cut(s) 442
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 1 cut(s) 99
Lsp1109I GCAGC 2 cut(s) 566, 599
LweI GCATC 3 cut(s) 288, 466, 543
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 2 cut(s) 65, 253
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 8 cut(s) 47, 50, 251, 260, 345, 483, 485, 605
MluCI AATT 2 cut(s) 154, 192
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 8 cut(s) 180, 328, 340, 351, 376, 528, 618, 621
MroXI GAANNNNTTC 2 cut(s) 179, 487
MseI TTAA 3 cut(s) 264, 272, 625
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 2 cut(s) 285, 564
MspR9I CCNGG 2 cut(s) 295, 444
MvaI CCWGG 2 cut(s) 295, 444
MvnI CGCG 2 cut(s) 57, 171
MwoI GCNNNNNNNGC 6 cut(s) 134, 143, 217, 285, 434, 560
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 1 cut(s) 77
NmuCI GTSAC 2 cut(s) 65, 253
NruI TCGCGA 1 cut(s) 57
PcsI WCGNNNNNNNCGW 2 cut(s) 61, 175
PdmI GAANNNNTTC 2 cut(s) 179, 487
PfeI GAWTC 3 cut(s) 61, 175, 180
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 2 cut(s) 556, 589
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 293, 442
PspGI CCWGG 2 cut(s) 293, 442
PspN4I GGNNCC 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 211
RruI TCGCGA 1 cut(s) 57
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
SaqAI TTAA 3 cut(s) 264, 272, 625
SatI GCNGC 2 cut(s) 555, 588
Sau3AI GATC 1 cut(s) 99
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 295, 444
SfaNI GCATC 3 cut(s) 288, 466, 543
SfcI CTRYAG 2 cut(s) 522, 582
SgrAI CRCCGGYG 1 cut(s) 284
Sse9I AATT 2 cut(s) 154, 192
SsiI CCGC 4 cut(s) 103, 128, 314, 338
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 293, 442
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 139, 178
TasI AATT 2 cut(s) 154, 192
TfiI GAWTC 3 cut(s) 61, 175, 180
Tru1I TTAA 3 cut(s) 264, 272, 625
Tru9I TTAA 3 cut(s) 264, 272, 625
TseFI GTSAC 2 cut(s) 65, 253
TseI GCWGC 2 cut(s) 554, 587
Tsp45I GTSAC 2 cut(s) 65, 253
TspDTI ATGAA 2 cut(s) 261, 345
XapI RAATTY 1 cut(s) 154
XmnI GAANNNNTTC 2 cut(s) 179, 487
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.