Rorug03G0038100

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000980
Physical Location & Seq
Reverse (-)
2989467 .. 3009065
19599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0038100.1

Sequence Viewer

Length: 972 bp
ATGGAGGACAAGTGTGCAATCAAGAATGATGTCACTGAATTAATAGGAAATACCCCAATGGTATATCTCAACAATGTCGTGGATGGTTGTGTAGCCAGAATTGCTGCCAAGCTAGAGACAATGGAGCCTTGCTCTAGTGTAAAAGACAGGATCGCTTATAGCATGATCAAGGATGCAGAGGATAAAGGTCTAATTACACCAGGGAAGACTGTTCTTATTGAGGTTACAAGTGGTAACACTGGCATTGGATTGGCATTCATTGCCGCAATCAAGGGTTACAAGCTCAAGATAAGCATGCCAGCTACAATGAGTCTTGAAAGAAGAATTGTGCTCCAAGCTTTTGGAGCTGAGGTCTTCATCGCAGATCCTGCCAGGGGCTTTGAGGGAGCTTTTGCCAAGGCAGACGAGCTGCTAAGTAACACACCTAATAGCTTTATACTTCAACAATCGGAAAACCCTGCCAATCCAAAGATTCATTACGAAACCACTGGCCCGGAGATATGGAGAGACTCAGGAGGGAAAGTTGATGTTTTTGTTTCAGCAATAGGGACTGGGGGTACTGTAACCGGTACAGGAAGTTTCCTTAAAGAGCAAAATCCAGATATTAAGGTGTATGGTGTAGAACCAGCTGAAAGTGCAGTATTGAATGGTGGCAAACCAGGGATGCATCTGATCCAAGGAATTGGTGCTGGTTTCATACCTCAAGTTCTGGATGTTAGCTTGCTGGATGAAGTTATTCAAGTATCCAGTGAGGAAGCAATTGAAACTGCTAAGCTGCTTGCGCTAAAAGAAGGTCTTTTGGTGGGGATTTCATCTGGTGCTGCAGCAGCAGCAGCAATAAAGTTGGCGAAGAAGCAAGAAAATGCTGGAAAATTAATAGTTGTTGTGTTTGCTAGTTCTGGAGAGCGTTATCTGTCTTCTCCACTCTTCAATACCATTAGGCACGAGGCAGAAAATATGAAGTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
MWFE

Protein Analysis

323

Amino Acids

34.23

Weight (kDa)

5.16

Isoelectric Point (pI)

23.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 11 - 297 8.7e-75 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 264
AclWI GGATC 3 cut(s) 158, 359, 667
AfaI GTAC 2 cut(s) 559, 571
AfiI CCNNNNNNNGG 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 7 cut(s) 317, 443, 646, 740, 764, 931, 968
AjnI CCWGG 3 cut(s) 199, 371, 658
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 110, 501
AlwI GGATC 3 cut(s) 158, 359, 667
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 1 cut(s) 490
ApeKI GCWGC 8 cut(s) 104, 409, 775, 821, 824, 827, 830, 833
AseI ATTAAT 2 cut(s) 41, 875
AsiGI ACCGGT 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 735
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 494
BauI CACGAG 1 cut(s) 944
BbsI GAAGAC 3 cut(s) 212, 346, 909
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 8 cut(s) 91, 396, 762, 808, 836, 839, 842, 845
BccI CCATC 1 cut(s) 77
BciT130I CCWGG 3 cut(s) 201, 373, 660
BciVI GTATCC 1 cut(s) 754
BclI TGATCA 1 cut(s) 165
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 110, 501
BfaI CTAG 3 cut(s) 113, 135, 894
BfmI CTRYAG 1 cut(s) 822
BfuI GTATCC 1 cut(s) 754
BisI GCNGC 9 cut(s) 105, 264, 410, 776, 822, 825, 828, 831, 834
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 9 cut(s) 106, 265, 411, 777, 823, 826, 829, 832, 835
Bme1390I CCNGG 4 cut(s) 201, 373, 494, 660
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 4 cut(s) 201, 373, 494, 660
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 3 cut(s) 163, 654, 676
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 3 cut(s) 212, 346, 909
BplI GAGNNNNNCTC 2 cut(s) 116, 148
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 771
BpuEI CTTGAG 2 cut(s) 269, 687
BpuMI CCSGG 1 cut(s) 494
BsaJI CCNNGG 5 cut(s) 200, 372, 396, 659, 676
BsaWI WCCGGW 1 cut(s) 566
BsaXI ACNNNNNCTCC 5 cut(s) 26, 336, 366, 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 4 cut(s) 244, 493, 556, 747
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 3 cut(s) 201, 373, 660
BseDI CCNNGG 5 cut(s) 200, 372, 396, 659, 676
BseGI GGATG 5 cut(s) 88, 178, 669, 718, 733
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 339, 525
BseNI ACTGG 4 cut(s) 244, 493, 556, 747
BseXI GCAGC 8 cut(s) 91, 396, 762, 808, 836, 839, 842, 845
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 1 cut(s) 492
BshTI ACCGGT 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 2 cut(s) 494, 567
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 2 cut(s) 110, 501
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 4 cut(s) 150, 165, 364, 672
Bsp1720I GCTNAGC 1 cut(s) 771
BspACI CCGC 1 cut(s) 264
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 2 cut(s) 340, 524
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 826
BspPI GGATC 3 cut(s) 158, 359, 667
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 4 cut(s) 244, 493, 556, 747
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 5 cut(s) 200, 372, 396, 659, 676
BssMI GATC 4 cut(s) 150, 165, 364, 672
BssSI CACGAG 1 cut(s) 944
BssT1I CCWWGG 2 cut(s) 396, 676
Bst2BI CACGAG 1 cut(s) 944
Bst2UI CCWGG 3 cut(s) 201, 373, 660
Bst4CI ACNGT 2 cut(s) 211, 562
Bst6I CTCTTC 1 cut(s) 932
BstAPI GCANNNNNTGC 2 cut(s) 260, 368
BstC8I GCNNGC 4 cut(s) 296, 300, 722, 780
BstDEI CTNAG 4 cut(s) 348, 413, 511, 771
BstF5I GGATG 5 cut(s) 88, 178, 669, 718, 733
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 4 cut(s) 153, 168, 367, 675
BstMAI GTCTC 2 cut(s) 110, 501
BstMBI GATC 4 cut(s) 150, 165, 364, 672
BstMWI GCNNNNNNNGC 9 cut(s) 101, 260, 344, 368, 635, 781, 827, 830, 833
BstNI CCWGG 3 cut(s) 201, 373, 660
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 4 cut(s) 199, 371, 492, 658
BstSFI CTRYAG 1 cut(s) 822
BstV1I GCAGC 8 cut(s) 91, 396, 762, 808, 836, 839, 842, 845
BstV2I GAAGAC 3 cut(s) 212, 346, 909
BstX2I RGATCY 1 cut(s) 364
BstXI CCANNNNNNTGG 2 cut(s) 341, 683
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 1 cut(s) 754
BsuRI GGCC 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 5 cut(s) 88, 178, 669, 718, 733
BtsIMutI CAGTG 4 cut(s) 33, 237, 486, 754
Cac8I GCNNGC 4 cut(s) 296, 300, 722, 780
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 558, 570
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 2 cut(s) 163, 295
CviQI GTAC 2 cut(s) 558, 570
DdeI CTNAG 4 cut(s) 348, 413, 511, 771
DpnI GATC 4 cut(s) 152, 167, 366, 674
DpnII GATC 4 cut(s) 150, 165, 364, 672
Eam1104I CTCTTC 1 cut(s) 932
EarI CTCTTC 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 396, 676
EcoRII CCWGG 3 cut(s) 199, 371, 658
EcoT14I CCWWGG 2 cut(s) 396, 676
EcoT22I ATGCAT 1 cut(s) 669
ErhI CCWWGG 2 cut(s) 396, 676
FaeI CATG 2 cut(s) 166, 298
FaiI YATR 9 cut(s) 64, 159, 164, 296, 437, 502, 615, 698, 959
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 562
FatI CATG 2 cut(s) 162, 294
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 9 cut(s) 105, 264, 410, 776, 822, 825, 828, 831, 834
FokI GGATG 5 cut(s) 95, 185, 676, 725, 740
Fsp4HI GCNGC 9 cut(s) 105, 264, 410, 776, 822, 825, 828, 831, 834
FspBI CTAG 3 cut(s) 113, 135, 894
GlaI GCGC 1 cut(s) 783
GluI GCNGC 9 cut(s) 105, 264, 410, 776, 822, 825, 828, 831, 834
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 1 cut(s) 492
HapII CCGG 2 cut(s) 494, 567
HhaI GCGC 1 cut(s) 784
Hin1II CATG 2 cut(s) 166, 298
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 3 cut(s) 310, 472, 509
HpaII CCGG 2 cut(s) 494, 567
Hpy188I TCNGA 2 cut(s) 451, 672
Hpy188III TCNNGA 7 cut(s) 22, 286, 314, 513, 599, 710, 900
HpyAV CCTTC 1 cut(s) 785
HpyCH4III ACNGT 2 cut(s) 211, 562
HpyCH4V TGCA 5 cut(s) 17, 176, 638, 667, 824
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 260, 344, 368, 635, 781, 827, 830, 833
HpyF3I CTNAG 4 cut(s) 348, 413, 511, 771
Hsp92II CATG 2 cut(s) 166, 298
HspAI GCGC 1 cut(s) 782
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 4 cut(s) 150, 165, 364, 672
LmnI GCTCC 4 cut(s) 124, 336, 344, 386
Lsp1109I GCAGC 8 cut(s) 91, 396, 762, 808, 836, 839, 842, 845
LweI GCATC 3 cut(s) 163, 654, 676
MaeI CTAG 3 cut(s) 113, 135, 894
MaeIII GTNAC 6 cut(s) 31, 223, 233, 275, 416, 562
MalI GATC 4 cut(s) 152, 167, 366, 674
MboI GATC 4 cut(s) 150, 165, 364, 672
MboII GAAGA 6 cut(s) 217, 333, 346, 862, 909, 919
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 7 cut(s) 38, 99, 192, 324, 681, 759, 872
MlyI GAGTC 2 cut(s) 319, 503
MnlI CCTC 8 cut(s) 172, 214, 343, 376, 509, 711, 745, 940
Mph1103I ATGCAT 1 cut(s) 669
MroXI GAANNNNTTC 1 cut(s) 735
MseI TTAA 4 cut(s) 41, 585, 606, 875
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 2 cut(s) 494, 567
MspR9I CCNGG 4 cut(s) 201, 373, 494, 660
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 3 cut(s) 201, 373, 660
MwoI GCNNNNNNNGC 9 cut(s) 101, 260, 344, 368, 635, 781, 827, 830, 833
NciI CCSGG 1 cut(s) 494
NdeII GATC 4 cut(s) 150, 165, 364, 672
NlaIII CATG 2 cut(s) 166, 298
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 669
NspI RCATGY 1 cut(s) 298
PaeI GCATGC 1 cut(s) 298
PctI GAATGC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 1 cut(s) 472
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 9 cut(s) 106, 265, 411, 777, 823, 826, 829, 832, 835
PleI GAGTC 2 cut(s) 318, 503
PpsI GAGTC 2 cut(s) 318, 503
PshBI ATTAAT 2 cut(s) 41, 875
Psp6I CCWGG 3 cut(s) 199, 371, 658
PspGI CCWGG 3 cut(s) 199, 371, 658
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 491
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 559, 571
RsaNI GTAC 2 cut(s) 558, 570
SaqAI TTAA 4 cut(s) 41, 585, 606, 875
SatI GCNGC 9 cut(s) 105, 264, 410, 776, 822, 825, 828, 831, 834
Sau3AI GATC 4 cut(s) 150, 165, 364, 672
Sau96I GGNCC 1 cut(s) 491
SchI GAGTC 2 cut(s) 319, 503
ScrFI CCNGG 4 cut(s) 201, 373, 494, 660
SduI GDGCHC 1 cut(s) 333
SfaNI GCATC 3 cut(s) 163, 654, 676
SfcI CTRYAG 1 cut(s) 822
SmlI CTYRAG 2 cut(s) 284, 702
SmoI CTYRAG 2 cut(s) 284, 702
SphI GCATGC 1 cut(s) 298
Sse9I AATT 7 cut(s) 38, 99, 192, 324, 681, 759, 872
SsiI CCGC 1 cut(s) 264
SspMI CTAG 3 cut(s) 113, 135, 894
StyD4I CCNGG 4 cut(s) 199, 371, 492, 658
StyI CCWWGG 2 cut(s) 396, 676
TaaI ACNGT 2 cut(s) 211, 562
TasI AATT 7 cut(s) 38, 99, 192, 324, 681, 759, 872
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 472
Tru1I TTAA 4 cut(s) 41, 585, 606, 875
Tru9I TTAA 4 cut(s) 41, 585, 606, 875
TscAI CASTG 4 cut(s) 40, 244, 493, 754
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 8 cut(s) 104, 409, 775, 821, 824, 827, 830, 833
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 6 cut(s) 247, 346, 464, 685, 744, 801
TspRI CASTG 4 cut(s) 40, 244, 493, 754
VspI ATTAAT 2 cut(s) 41, 875
XceI RCATGY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 3 cut(s) 113, 135, 894
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.