Rroxscaffold_3G00253100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000980
Physical Location & Seq
Forward (+)
47162692 .. 47165387
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253100.1

Sequence Viewer

Length: 1401 bp
ATGGCAATAGCACTGCTGCTGACAGCTTTATTTGTTTGTATATCAAGCCTGGTATTGAGAGCGCTTAACCAGTTGTGGTTTAAGCCTCGAAGAATTAGGTCAGTGCTTTATAAGCAAGGCATTCGAGGACCGAGACCAAGCTTCCCTTTCGGAAACATTCCTGAGATGCAGAAGATGCAGTCCTCCATCAAGATTCCTAGTGATGCCCAGCAACATGTGCACCACAATTGGGTTCCCTCCATATTCCCATATCTTCACAAATGGGAACAAAAGTACGGTCCAATATACATGTACTCCACAGGGAGCAAGCAACACCTTTATGGCATGGTGGGTTTGATGCTGGAATCTACAATGAAAATAATGAAGGAATGGGAGAGCCGTATACAAGAAGTTGGTGAGGATTCGAATGTTCTTGACATGAGTATTGATAAGGATTTGAAGACCCTCTCCCGAGATATCATCTCCAGGGCTTGTTTTAGCAGCTCTTACGCTCAAGGAAGCCAGATTTTTGAAAAGATTTCTCTCATGCAGGAAGCTTTGTCGAAACCAAGTTTGCTTTTCGGCTTTCTAAATTTCAGGTTTCTTCCTTTTAACAATGACAAGAAGATATGGAATTTAAGGAGAGATGTGGACGCTATGTTGCTCGAAATAGTGAGGCATCGCCAATCCGGTGGTGGTGTCCACAATGCACAAAAAGACCTACTACAAGTGCTACTAGAAAGTGCTGCTGCTGCTAACAATGGGAAGCATACACCTAAAACTGAACGTTTCGTTTTGGACAATTGTAGAACTATCTACTTTGCTGGCTCTGAGACCACTTCTCTGACTGCATCATGGACCTTGATGCTTCTTTCTTTGCACCCCGAGTGGCAAGAGCGACTTCGTGCTGAGATTATTGGCATTTGTGGGGATCATGATCGACATGAACTCCATCGTTGCTTACAAGACATGGATACGTTAAGCAAATTAAAAATGTTAACAATGGTGATTCAAGAGAGCCTGCGTCTTTACGCACCAGCTATCCTAACACCACGGCAAGCTCTAGCAAACATGAAATTGGGGGATCTTGATGTGCCACAAGACATGATCATATGGATAGCCATTGCATCGTTGCACCGTGACCCCAAAAATTGGGGTCCAGATGCTAATGAGTTCAAGCCGGAGAGATTTGAAAATGGCGTAACAGAAGCATGCAAATTTCCAAATGCATATCTGCCATTTGGTTTTGGGAGTCGACTGTGTATAGGCCAGACGTTTGCGATGATGGAGCTGAAGATAGTACTCTCTCTATTACTATCGAAGTTCTCATTTTCGCTTTCACCAAACTATCACCACTCCCCTGTTTCGAAAATGGTATTGGTGCCAGAACATGGCATCAATCTCCTTGTTAGGCGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

466

Amino Acids

53.29

Weight (kDa)

9.16

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 109 - 461 4.5e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 1360
AccB7I CCANNNNNTGG 2 cut(s) 1131, 1370
AccI GTMKAC 2 cut(s) 382, 1234
AclI AACGTT 1 cut(s) 766
AclWI GGATC 2 cut(s) 918, 1071
AcsI RAATTY 3 cut(s) 571, 613, 1196
AcuI CTGAAG 1 cut(s) 1292
AfaI GTAC 3 cut(s) 275, 293, 1281
AfeI AGCGCT 1 cut(s) 63
AfiI CCNNNNNNNGG 3 cut(s) 229, 1131, 1370
AflIII ACRYGT 2 cut(s) 214, 288
AgsI TTSAA 5 cut(s) 439, 512, 992, 1156, 1172
AjnI CCWGG 2 cut(s) 48, 464
AjuI GAANNNNNNNTTGG 2 cut(s) 1340, 1372
AluBI AGCT 7 cut(s) 26, 141, 483, 536, 1019, 1040, 1270
AluI AGCT 7 cut(s) 26, 141, 483, 536, 1019, 1040, 1270
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 127, 806
Alw44I GTGCAC 1 cut(s) 218
AlwI GGATC 2 cut(s) 918, 1071
Ama87I CYCGRG 2 cut(s) 450, 863
Aor51HI AGCGCT 1 cut(s) 63
AoxI GGCC 1 cut(s) 1246
ApaLI GTGCAC 1 cut(s) 218
ApeKI GCWGC 5 cut(s) 16, 480, 725, 728, 731
ApoI RAATTY 3 cut(s) 571, 613, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 4 cut(s) 128, 278, 837, 1136
AsuHPI GGTGA 4 cut(s) 407, 997, 1311, 1322
AsuII TTCGAA 2 cut(s) 404, 1346
AvaI CYCGRG 2 cut(s) 450, 863
AvaII GGWCC 4 cut(s) 128, 278, 837, 1136
BaeGI GKGCMC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 1360
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 5 cut(s) 3, 492, 712, 715, 718
BccI CCATC 3 cut(s) 194, 939, 1258
BceAI ACGGC 2 cut(s) 363, 1049
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 2 cut(s) 50, 466
BciVI GTATCC 1 cut(s) 946
BclI TGATCA 1 cut(s) 1086
BcoDI GTCTC 2 cut(s) 127, 806
BfaI CTAG 3 cut(s) 198, 716, 1043
BfoI RGCGCY 1 cut(s) 65
BfuI GTATCC 1 cut(s) 946
BisI GCNGC 5 cut(s) 17, 481, 726, 729, 732
BlsI GCNGC 5 cut(s) 18, 482, 727, 730, 733
BmcAI AGTACT 1 cut(s) 1281
Bme1390I CCNGG 2 cut(s) 50, 466
Bme18I GGWCC 4 cut(s) 128, 278, 837, 1136
BmeT110I CYCGRG 2 cut(s) 450, 863
BmgT120I GGNCC 4 cut(s) 128, 278, 837, 1136
BmiI GGNNCC 3 cut(s) 234, 1137, 1362
BmrFI CCNGG 2 cut(s) 50, 466
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 448
Bpu14I TTCGAA 2 cut(s) 404, 1346
BpuEI CTTGAG 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 2 cut(s) 127, 806
BsaJI CCNNGG 2 cut(s) 465, 1031
BsaWI WCCGGW 1 cut(s) 668
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 912, 942
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 1131, 1370
Bse1I ACTGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 50, 466
BseDI CCNNGG 2 cut(s) 465, 1031
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 229, 1131, 1370
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 3 cut(s) 153, 801, 879
BseNI ACTGG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 5 cut(s) 3, 492, 712, 715, 718
BseYI CCCAGC 1 cut(s) 207
BshFI GGCC 1 cut(s) 1248
BshNI GGYRCC 1 cut(s) 1360
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 2 cut(s) 450, 863
BsiSI CCGG 2 cut(s) 669, 1160
BslI CCNNNNNNNGG 3 cut(s) 229, 1131, 1370
BsmAI GTCTC 2 cut(s) 127, 806
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 1 cut(s) 1248
Bso31I GGTCTC 2 cut(s) 127, 806
BsoBI CYCGRG 2 cut(s) 450, 863
Bsp119I TTCGAA 2 cut(s) 404, 1346
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 4 cut(s) 910, 916, 1063, 1086
BspANI GGCC 1 cut(s) 1248
BspCNI CTCAG 3 cut(s) 154, 802, 880
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 3 cut(s) 234, 1137, 1362
BspPI GGATC 2 cut(s) 918, 1071
BspT104I TTCGAA 2 cut(s) 404, 1346
BspT107I GGYRCC 1 cut(s) 1360
BspTNI GGTCTC 2 cut(s) 127, 806
BsrDI GCAATG 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 465, 1031
BssMI GATC 4 cut(s) 910, 916, 1063, 1086
BssNAI GTATAC 1 cut(s) 383
Bst1107I GTATAC 1 cut(s) 383
Bst2UI CCWGG 2 cut(s) 50, 466
Bst4CI ACNGT 3 cut(s) 278, 1118, 1239
BstAPI GCANNNNNTGC 2 cut(s) 175, 217
BstBI TTCGAA 2 cut(s) 404, 1346
BstC8I GCNNGC 5 cut(s) 308, 805, 1001, 1038, 1192
BstDEI CTNAG 3 cut(s) 162, 810, 888
BstDSI CCRYGG 1 cut(s) 1031
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 4 cut(s) 913, 919, 1066, 1089
BstMAI GTCTC 2 cut(s) 127, 806
BstMBI GATC 4 cut(s) 910, 916, 1063, 1086
BstMWI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 731
BstNI CCWGG 2 cut(s) 50, 466
BstNSI RCATGY 3 cut(s) 218, 292, 1194
BstSCI CCNGG 2 cut(s) 48, 464
BstSLI GKGCMC 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 3, 492, 712, 715, 718
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 1063
BstXI CCANNNNNNTGG 1 cut(s) 671
BstYI RGATCY 1 cut(s) 1063
BstZ17I GTATAC 1 cut(s) 383
BsuI GTATCC 1 cut(s) 946
BsuRI GGCC 1 cut(s) 1248
BtgI CCRYGG 1 cut(s) 1031
BtgZI GCGATG 2 cut(s) 644, 1274
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 2 cut(s) 11, 108
Cac8I GCNNGC 5 cut(s) 308, 805, 1001, 1038, 1192
CciI TCATGA 1 cut(s) 913
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 4 cut(s) 128, 278, 837, 1136
CseI GACGC 2 cut(s) 641, 992
Csp6I GTAC 3 cut(s) 274, 292, 1280
CviQI GTAC 3 cut(s) 274, 292, 1280
DdeI CTNAG 3 cut(s) 162, 810, 888
DpnI GATC 4 cut(s) 912, 918, 1065, 1088
DpnII GATC 4 cut(s) 910, 916, 1063, 1086
Eco31I GGTCTC 2 cut(s) 127, 806
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 4 cut(s) 128, 278, 837, 1136
Eco47III AGCGCT 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 1292
Eco88I CYCGRG 2 cut(s) 450, 863
EcoRII CCWGG 2 cut(s) 48, 464
EcoRV GATATC 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 1210
FalI AAGNNNNNCTT 4 cut(s) 130, 162, 864, 896
FauNDI CATATG 1 cut(s) 1091
FbaI TGATCA 1 cut(s) 1086
FblI GTMKAC 2 cut(s) 382, 1234
Fnu4HI GCNGC 5 cut(s) 17, 481, 726, 729, 732
Fsp4HI GCNGC 5 cut(s) 17, 481, 726, 729, 732
FspBI CTAG 3 cut(s) 198, 716, 1043
GlaI GCGC 1 cut(s) 63
GluI GCNGC 5 cut(s) 17, 481, 726, 729, 732
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 448
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 1248
HapII CCGG 2 cut(s) 669, 1160
HgaI GACGC 2 cut(s) 641, 992
HhaI GCGC 1 cut(s) 64
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 2 cut(s) 978, 1235
HindII GTYRAC 2 cut(s) 978, 1235
HindIII AAGCTT 2 cut(s) 139, 534
HinfI GANTC 5 cut(s) 193, 344, 401, 988, 1231
HpaI GTTAAC 1 cut(s) 978
HpaII CCGG 2 cut(s) 669, 1160
HphI GGTGA 4 cut(s) 407, 997, 1311, 1322
Hpy166II GTNNAC 6 cut(s) 220, 383, 631, 682, 978, 1235
Hpy188I TCNGA 3 cut(s) 152, 811, 825
Hpy188III TCNNGA 8 cut(s) 161, 190, 413, 450, 914, 992, 1067, 1139
Hpy8I GTNNAC 6 cut(s) 220, 383, 631, 682, 978, 1235
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 3 cut(s) 278, 1118, 1239
HpyCH4IV ACGT 3 cut(s) 766, 956, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 731
HpyF3I CTNAG 3 cut(s) 162, 810, 888
HpySE526I ACGT 3 cut(s) 766, 956, 1253
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 1086
KspAI GTTAAC 1 cut(s) 978
Kzo9I GATC 4 cut(s) 910, 916, 1063, 1086
LmnI GCTCC 2 cut(s) 303, 1267
Lsp1109I GCAGC 5 cut(s) 3, 492, 712, 715, 718
MaeI CTAG 3 cut(s) 198, 716, 1043
MaeII ACGT 3 cut(s) 766, 956, 1253
MaeIII GTNAC 2 cut(s) 1118, 1180
MalI GATC 4 cut(s) 912, 918, 1065, 1088
MboI GATC 4 cut(s) 910, 916, 1063, 1086
MboII GAAGA 7 cut(s) 102, 184, 245, 451, 575, 616, 1285
MfeI CAATTG 2 cut(s) 226, 781
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 9 cut(s) 93, 226, 571, 613, 781, 965, 1055, 1129, 1196
MlyI GAGTC 1 cut(s) 1240
MnlI CCTC 7 cut(s) 96, 119, 193, 247, 391, 455, 648
Mph1103I ATGCAT 1 cut(s) 1210
MseI TTAA 7 cut(s) 66, 81, 591, 617, 959, 968, 977
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 2 cut(s) 669, 1160
MspR9I CCNGG 2 cut(s) 50, 466
MunI CAATTG 2 cut(s) 226, 781
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 2 cut(s) 50, 466
MwoI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 731
NdeI CATATG 1 cut(s) 1091
NdeII GATC 4 cut(s) 910, 916, 1063, 1086
NlaIV GGNNCC 3 cut(s) 234, 1137, 1362
NmuCI GTSAC 1 cut(s) 1118
NsiI ATGCAT 1 cut(s) 1210
NspI RCATGY 3 cut(s) 218, 292, 1194
NspV TTCGAA 2 cut(s) 404, 1346
PaeI GCATGC 1 cut(s) 1194
PagI TCATGA 1 cut(s) 913
PciI ACATGT 2 cut(s) 214, 288
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 4 cut(s) 193, 344, 401, 988
PflMI CCANNNNNTGG 2 cut(s) 1131, 1370
PkrI GCNGC 5 cut(s) 18, 482, 727, 730, 733
PleI GAGTC 1 cut(s) 1239
PpsI GAGTC 1 cut(s) 1239
PscI ACATGT 2 cut(s) 214, 288
PsiI TTATAA 1 cut(s) 111
Psp1406I AACGTT 1 cut(s) 766
Psp6I CCWGG 2 cut(s) 48, 464
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 2 cut(s) 48, 464
PspN4I GGNNCC 3 cut(s) 234, 1137, 1362
PspPI GGNCC 4 cut(s) 128, 278, 837, 1136
PsuI RGATCY 1 cut(s) 1063
RsaI GTAC 3 cut(s) 275, 293, 1281
RsaNI GTAC 3 cut(s) 274, 292, 1280
RseI CAYNNNNRTG 1 cut(s) 318
SalI GTCGAC 1 cut(s) 1233
SaqAI TTAA 7 cut(s) 66, 81, 591, 617, 959, 968, 977
SatI GCNGC 5 cut(s) 17, 481, 726, 729, 732
Sau3AI GATC 4 cut(s) 910, 916, 1063, 1086
Sau96I GGNCC 4 cut(s) 128, 278, 837, 1136
ScaI AGTACT 1 cut(s) 1281
SchI GAGTC 1 cut(s) 1240
ScrFI CCNGG 2 cut(s) 50, 466
SduI GDGCHC 1 cut(s) 222
SfuI TTCGAA 2 cut(s) 404, 1346
SinI GGWCC 4 cut(s) 128, 278, 837, 1136
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 492
SmoI CTYRAG 1 cut(s) 492
SphI GCATGC 1 cut(s) 1194
Sse9I AATT 9 cut(s) 93, 226, 571, 613, 781, 965, 1055, 1129, 1196
SspMI CTAG 3 cut(s) 198, 716, 1043
StyD4I CCNGG 2 cut(s) 48, 464
TaaI ACNGT 3 cut(s) 278, 1118, 1239
TaiI ACGT 3 cut(s) 769, 959, 1256
TaqI TCGA 9 cut(s) 88, 124, 404, 542, 645, 919, 1234, 1298, 1346
TaqII GACCGA 1 cut(s) 145
TasI AATT 9 cut(s) 93, 226, 571, 613, 781, 965, 1055, 1129, 1196
TatI WGTACW 2 cut(s) 291, 1279
TfiI GAWTC 4 cut(s) 193, 344, 401, 988
Tru1I TTAA 7 cut(s) 66, 81, 591, 617, 959, 968, 977
Tru9I TTAA 7 cut(s) 66, 81, 591, 617, 959, 968, 977
TscAI CASTG 2 cut(s) 18, 108
TseFI GTSAC 1 cut(s) 1118
TseI GCWGC 5 cut(s) 16, 480, 725, 728, 731
Tsp45I GTSAC 1 cut(s) 1118
TspDTI ATGAA 4 cut(s) 368, 377, 939, 1067
TspRI CASTG 2 cut(s) 18, 108
Van91I CCANNNNNTGG 2 cut(s) 1131, 1370
VneI GTGCAC 1 cut(s) 218
VpaK11BI GGWCC 4 cut(s) 128, 278, 837, 1136
XapI RAATTY 3 cut(s) 571, 613, 1196
XceI RCATGY 3 cut(s) 218, 292, 1194
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XmiI GTMKAC 2 cut(s) 382, 1234
XspI CTAG 3 cut(s) 198, 716, 1043
ZrmI AGTACT 1 cut(s) 1281
Zsp2I ATGCAT 1 cut(s) 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.