FvH4_6g09320

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000980
Physical Location & Seq
Forward (+)
5529203 .. 5532955
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09320.t1

Sequence Viewer

Length: 1599 bp
ATGGAAGTGGATGCTCTACCGATATGGTGGTCTGTTTTGTTTATTGGTGTATCGAGCTTGGTAATGAAATTGCTTAACAAGTTTTGGTTCGAGCCTAGAAGGATTCGAACAGTGCTTTCGAAACAAGGGATTAGAGGACCACCGCCCACCTTCCTTTTAGGCAATGTCTCCGAGATGCAAAAGATTCAATCCGCCCTGATCAACAACACGCATGAAGCGTTGGATAGTCACCGCCATCTCAACCACAATTGGCTTCCTTTTGTATTTCCACATATTTATGAATGGGCAAAAGAGTACGGTCCTATATTCATGTACTCAACAGGGGTCAACCACCACCTGTATGTGAGCGATCCCGAATTGTTAATGGAGATAAAACAAAACAACTCCCTAAATCTAGGGAAACCTCCATATCTGGGTAAACCACTCCAACCCTTGTTAGGTGATGGCCTTATTCGCAGTAATGGTGAAAAATGGGCTGTACAAAGGAATCTTCTTGCTCCGGAATTCTTCCTTGCCAAGGTTAAGGGCATGGTGGGTGTGATGGTGGAATCTACAACATCAATGATCAATACATGGGATTGTCGTATACTAGAAAGCAAATCTGGCATTGTGGACATGGAAGTCGATGACGAGTTGAAGACACTATCTGCAGATATCATATCAAGAGCATGTTTCGGCAGTTCTCACTCCTTTGGAAATCAGATACTTGAAAAGATTGCTCAAATGGTGGACATTTTGGGGAAACCAAGCTTACTATATGGCTTTCTTGATTTTAGCTGGGTGCTTCCTAACAAGAAGTTATGGAATCTAAGGAAAGAAGTGGACTTTATGTTGCTCAAGTTAGTGAGGGATCGCCAGGTTAAACGACAATCAGGTGCCGCAACAGAAAATGACCTATTACAAATGCTACTCGATATTGCTTCTACTAACACCACTGACATTCCTACACGTCGAAGAGAAGCTTTCATTCTAGACAATTGCAGAACTATCTACTTTGCAAGCTCTGTAGCCACTTCTCTTACGGCATCATGGACCTTGGTGCTTCTGTCGCAGCACCCCGAATGGCAGGACCGCATTCGTGCGGAGATCGTTGAGGTTTGTGGGGGTCCGGATAAACTCCATCATTGCTTGCGCAACCCGGACGTGTTGCGCAAGTTCAAAGTGCTGACTATGGTGATTCAAGAGAGTATGCGTCTTTATGCATCTGCAGTTCTACTTGTAAGAGCTGCCCTGGAGAATGTGAAGTTGGGCAACCTTGATGTGCCAAAAGGCACTATCATCCAGGTATGCAGTGCTACATTACACCGCGACCCTGAAAATTGGGGTCCGGATGCTGATGAGTTTAAGCCGGAGAGGTTTGCAAATGGGATATCTAATGCGTGCAAGCATCCCCAAGCTTATGTGCCATTTGGTTTTGGGCATCGAGTGTGCATTGGACAGACTTTTGCTATAACACAACTGAAGATAGAGCTCTCTCTAATCTTGTCCAAGTTCGCATTTTCCCTTTCTCCAAACTACCGCCATGCACCTTCTCACAAAATGTTACTGATGCCTAAACATGGAATCAGACTCCTTGTTAAGCCTGTGCAAGCAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

533

Amino Acids

60.25

Weight (kDa)

9.01

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 93 - 525 5.9e-73 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 620
Acc16I TGCGCA 2 cut(s) 1133, 1151
AccB1I GGYRCC 1 cut(s) 875
AccI GTMKAC 1 cut(s) 586
AccII CGCG 1 cut(s) 1308
AccIII TCCGGA 3 cut(s) 499, 1108, 1327
AciI CCGC 8 cut(s) 143, 192, 232, 879, 1072, 1082, 1306, 1519
AclWI GGATC 2 cut(s) 344, 858
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 1481
AfaI GTAC 3 cut(s) 296, 314, 480
AfiI CCNNNNNNNGG 4 cut(s) 413, 437, 517, 1559
AflIII ACRYGT 2 cut(s) 947, 1143
AgsI TTSAA 5 cut(s) 188, 637, 710, 1159, 1181
AjiI CACGTC 2 cut(s) 950, 1144
AjnI CCWGG 3 cut(s) 855, 1230, 1281
AjuI GAANNNNNNNTTGG 2 cut(s) 1229, 1261
AluBI AGCT 8 cut(s) 57, 750, 777, 962, 1002, 1226, 1397, 1471
AluI AGCT 8 cut(s) 57, 750, 777, 962, 1002, 1226, 1397, 1471
Alw21I GWGCWC 1 cut(s) 1473
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 2 cut(s) 344, 858
Aor13HI TCCGGA 3 cut(s) 499, 1108, 1327
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 2 cut(s) 1051, 1226
ApoI RAATTY 1 cut(s) 503
AspLEI GCGC 2 cut(s) 1134, 1152
AspS9I GGNCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
AsuC2I CCSGG 1 cut(s) 1139
AsuHPI GGTGA 4 cut(s) 221, 452, 476, 1186
AsuII TTCGAA 2 cut(s) 106, 119
AvaII GGWCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
BanI GGYRCC 1 cut(s) 875
BanII GRGCYC 1 cut(s) 1473
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 1473
BbvI GCAGC 2 cut(s) 1063, 1213
BccI CCATC 4 cut(s) 243, 437, 535, 1128
BceAI ACGGC 1 cut(s) 1038
BciT130I CCWGG 3 cut(s) 857, 1232, 1283
BclI TGATCA 2 cut(s) 198, 564
BcnI CCSGG 1 cut(s) 1139
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 5 cut(s) 96, 395, 590, 971, 1597
BfmI CTRYAG 3 cut(s) 648, 1005, 1206
BisI GCNGC 3 cut(s) 879, 1052, 1227
BlsI GCNGC 3 cut(s) 880, 1053, 1228
Bme1390I CCNGG 4 cut(s) 857, 1139, 1232, 1283
Bme18I GGWCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
BmgBI CACGTC 2 cut(s) 950, 1144
BmgT120I GGNCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
BmiI GGNNCC 3 cut(s) 877, 1107, 1326
BmrFI CCNGG 4 cut(s) 857, 1139, 1232, 1283
BmsI GCATC 7 cut(s) 165, 1034, 1211, 1321, 1396, 1429, 1539
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 1253
Bpu14I TTCGAA 2 cut(s) 106, 119
BpuEI CTTGAG 1 cut(s) 821
BpuMI CCSGG 1 cut(s) 1139
BsaJI CCNNGG 3 cut(s) 516, 1035, 1230
BsaWI WCCGGW 3 cut(s) 499, 1108, 1327
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 437, 517, 1559
Bse3DI GCAATG 2 cut(s) 169, 1123
BseAI TCCGGA 3 cut(s) 499, 1108, 1327
BseBI CCWGG 3 cut(s) 857, 1232, 1283
BseDI CCNNGG 3 cut(s) 516, 1035, 1230
BseGI GGATG 4 cut(s) 16, 1278, 1336, 1387
BseLI CCNNNNNNNGG 4 cut(s) 413, 437, 517, 1559
BseMI GCAATG 2 cut(s) 169, 1123
BseXI GCAGC 2 cut(s) 1063, 1213
BseYI CCCAGC 1 cut(s) 777
Bsh1236I CGCG 1 cut(s) 1308
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 1473
BsiSI CCGG 5 cut(s) 500, 1109, 1139, 1328, 1349
BslI CCNNNNNNNGG 4 cut(s) 413, 437, 517, 1559
BsmAI GTCTC 1 cut(s) 172
BsmI GAATGC 1 cut(s) 1074
BsnI GGCC 1 cut(s) 447
Bsp119I TTCGAA 2 cut(s) 106, 119
Bsp1286I GDGCHC 1 cut(s) 1473
Bsp13I TCCGGA 3 cut(s) 499, 1108, 1327
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 5 cut(s) 198, 349, 564, 850, 1086
BspACI CCGC 8 cut(s) 143, 192, 232, 879, 1072, 1082, 1306, 1519
BspANI GGCC 1 cut(s) 447
BspEI TCCGGA 3 cut(s) 499, 1108, 1327
BspFNI CGCG 1 cut(s) 1308
BspLI GGNNCC 3 cut(s) 877, 1107, 1326
BspMAI CTGCAG 2 cut(s) 652, 1210
BspPI GGATC 2 cut(s) 344, 858
BspT104I TTCGAA 2 cut(s) 106, 119
BspT107I GGYRCC 1 cut(s) 875
BsrDI GCAATG 2 cut(s) 169, 1123
BsrGI TGTACA 1 cut(s) 478
BssECI CCNNGG 3 cut(s) 516, 1035, 1230
BssMI GATC 5 cut(s) 198, 349, 564, 850, 1086
BssNAI GTATAC 1 cut(s) 587
BssT1I CCWWGG 2 cut(s) 516, 1035
Bst1107I GTATAC 1 cut(s) 587
Bst2UI CCWGG 3 cut(s) 857, 1232, 1283
Bst4CI ACNGT 2 cut(s) 112, 299
Bst6I CTCTTC 1 cut(s) 949
BstAUI TGTACA 1 cut(s) 478
BstBI TTCGAA 2 cut(s) 106, 119
BstC8I GCNNGC 6 cut(s) 1000, 1130, 1381, 1385, 1590, 1594
BstDEI CTNAG 1 cut(s) 809
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 4 cut(s) 16, 1278, 1336, 1387
BstFNI CGCG 1 cut(s) 1308
BstHHI GCGC 2 cut(s) 1134, 1152
BstKTI GATC 5 cut(s) 201, 352, 567, 853, 1089
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 5 cut(s) 198, 349, 564, 850, 1086
BstMWI GCNNNNNNNGC 3 cut(s) 453, 603, 1048
BstNI CCWGG 3 cut(s) 857, 1232, 1283
BstNSI RCATGY 1 cut(s) 672
BstSCI CCNGG 4 cut(s) 855, 1137, 1230, 1281
BstSFI CTRYAG 3 cut(s) 648, 1005, 1206
BstUI CGCG 1 cut(s) 1308
BstV1I GCAGC 2 cut(s) 1063, 1213
BstV2I GAAGAC 1 cut(s) 644
BstZ17I GTATAC 1 cut(s) 587
BsuRI GGCC 1 cut(s) 447
BtrI CACGTC 2 cut(s) 950, 1144
BtsCI GGATG 4 cut(s) 16, 1278, 1336, 1387
BtsI GCAGTG 1 cut(s) 1297
BtsIMutI CAGTG 3 cut(s) 117, 933, 1297
Cac8I GCNNGC 6 cut(s) 1000, 1130, 1381, 1385, 1590, 1594
CfoI GCGC 2 cut(s) 1134, 1152
Cfr13I GGNCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
CseI GACGC 1 cut(s) 1181
Csp6I GTAC 3 cut(s) 295, 313, 479
CviAII CATG 9 cut(s) 212, 310, 529, 573, 616, 669, 1029, 1523, 1559
CviQI GTAC 3 cut(s) 295, 313, 479
DdeI CTNAG 1 cut(s) 809
DpnI GATC 5 cut(s) 200, 351, 566, 852, 1088
DpnII GATC 5 cut(s) 198, 349, 564, 850, 1086
DrdI GACNNNNNNGTC 1 cut(s) 620
DseDI GACNNNNNNGTC 1 cut(s) 620
Eam1104I CTCTTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 949
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 1471
Eco130I CCWWGG 2 cut(s) 516, 1035
Eco24I GRGCYC 1 cut(s) 1473
Eco32I GATATC 2 cut(s) 655, 1371
Eco47I GGWCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 1 cut(s) 1481
EcoICRI GAGCTC 1 cut(s) 1471
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 3 cut(s) 855, 1230, 1281
EcoRV GATATC 2 cut(s) 655, 1371
EcoT14I CCWWGG 2 cut(s) 516, 1035
EcoT22I ATGCAT 1 cut(s) 1204
EcoT38I GRGCYC 1 cut(s) 1473
ErhI CCWWGG 2 cut(s) 516, 1035
FaeI CATG 9 cut(s) 215, 313, 532, 576, 619, 672, 1032, 1526, 1562
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FatI CATG 9 cut(s) 211, 309, 528, 572, 615, 668, 1028, 1522, 1558
FbaI TGATCA 2 cut(s) 198, 564
FblI GTMKAC 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 879, 1052, 1227
FokI GGATG 4 cut(s) 23, 1265, 1343, 1374
FriOI GRGCYC 1 cut(s) 1473
Fsp4HI GCNGC 3 cut(s) 879, 1052, 1227
FspBI CTAG 5 cut(s) 96, 395, 590, 971, 1597
FspI TGCGCA 2 cut(s) 1133, 1151
GlaI GCGC 2 cut(s) 1133, 1151
GluI GCNGC 3 cut(s) 879, 1052, 1227
GsaI CCCAGC 1 cut(s) 781
GsuI CTGGAG 1 cut(s) 1253
HaeIII GGCC 1 cut(s) 447
HapII CCGG 5 cut(s) 500, 1109, 1139, 1328, 1349
HgaI GACGC 1 cut(s) 1181
HhaI GCGC 2 cut(s) 1134, 1152
Hin1II CATG 9 cut(s) 215, 313, 532, 576, 619, 672, 1032, 1526, 1562
Hin6I GCGC 2 cut(s) 1132, 1150
HinP1I GCGC 2 cut(s) 1132, 1150
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 3 cut(s) 748, 960, 1395
HinfI GANTC 8 cut(s) 103, 184, 487, 548, 805, 1177, 1563, 1569
HpaII CCGG 5 cut(s) 500, 1109, 1139, 1328, 1349
HphI GGTGA 4 cut(s) 221, 452, 476, 1186
Hpy166II GTNNAC 6 cut(s) 328, 419, 587, 613, 730, 823
Hpy188I TCNGA 3 cut(s) 172, 702, 1568
Hpy188III TCNNGA 8 cut(s) 353, 500, 663, 767, 971, 1109, 1181, 1328
Hpy8I GTNNAC 6 cut(s) 328, 419, 587, 613, 730, 823
Hpy99I CGWCG 1 cut(s) 954
HpyAV CCTTC 3 cut(s) 93, 160, 1539
HpyCH4III ACNGT 2 cut(s) 112, 299
HpyCH4IV ACGT 2 cut(s) 949, 1143
HpyF10VI GCNNNNNNNGC 3 cut(s) 453, 603, 1048
HpyF3I CTNAG 1 cut(s) 809
HpySE526I ACGT 2 cut(s) 949, 1143
Hsp92II CATG 9 cut(s) 215, 313, 532, 576, 619, 672, 1032, 1526, 1562
HspAI GCGC 2 cut(s) 1132, 1150
Kpn2I TCCGGA 3 cut(s) 499, 1108, 1327
Ksp22I TGATCA 2 cut(s) 198, 564
Kzo9I GATC 5 cut(s) 198, 349, 564, 850, 1086
LmnI GCTCC 1 cut(s) 502
Lsp1109I GCAGC 2 cut(s) 1063, 1213
LweI GCATC 7 cut(s) 165, 1034, 1211, 1321, 1396, 1429, 1539
MaeI CTAG 5 cut(s) 96, 395, 590, 971, 1597
MaeII ACGT 2 cut(s) 949, 1143
MaeIII GTNAC 2 cut(s) 227, 1542
MalI GATC 5 cut(s) 200, 351, 566, 852, 1088
MboI GATC 5 cut(s) 198, 349, 564, 850, 1086
MboII GAAGA 5 cut(s) 482, 499, 649, 966, 1474
MfeI CAATTG 2 cut(s) 247, 976
MhlI GDGCHC 1 cut(s) 1473
MluCI AATT 6 cut(s) 68, 247, 356, 503, 976, 1318
MlyI GAGTC 1 cut(s) 1563
MmeI TCCRAC 2 cut(s) 201, 451
MnlI CCTC 5 cut(s) 128, 414, 840, 1087, 1347
Mph1103I ATGCAT 1 cut(s) 1204
MroI TCCGGA 3 cut(s) 499, 1108, 1327
MseI TTAA 6 cut(s) 75, 362, 522, 861, 1344, 1578
MslI CAYNNNNRTG 2 cut(s) 276, 339
MspI CCGG 5 cut(s) 500, 1109, 1139, 1328, 1349
MspR9I CCNGG 4 cut(s) 857, 1139, 1232, 1283
MunI CAATTG 2 cut(s) 247, 976
Mva1269I GAATGC 1 cut(s) 1074
MvaI CCWGG 3 cut(s) 857, 1232, 1283
MvnI CGCG 1 cut(s) 1308
MwoI GCNNNNNNNGC 3 cut(s) 453, 603, 1048
NciI CCSGG 1 cut(s) 1139
NdeII GATC 5 cut(s) 198, 349, 564, 850, 1086
NlaIII CATG 9 cut(s) 215, 313, 532, 576, 619, 672, 1032, 1526, 1562
NlaIV GGNNCC 3 cut(s) 877, 1107, 1326
NmuCI GTSAC 1 cut(s) 227
NsbI TGCGCA 2 cut(s) 1133, 1151
NsiI ATGCAT 1 cut(s) 1204
NspI RCATGY 1 cut(s) 672
NspV TTCGAA 2 cut(s) 106, 119
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PctI GAATGC 1 cut(s) 1074
PfeI GAWTC 7 cut(s) 103, 184, 487, 548, 805, 1177, 1563
PkrI GCNGC 3 cut(s) 880, 1053, 1228
PleI GAGTC 1 cut(s) 1563
PpsI GAGTC 1 cut(s) 1563
Psp124BI GAGCTC 1 cut(s) 1473
Psp6I CCWGG 3 cut(s) 855, 1230, 1281
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 3 cut(s) 855, 1230, 1281
PspN4I GGNNCC 3 cut(s) 877, 1107, 1326
PspPI GGNCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
PstI CTGCAG 2 cut(s) 652, 1210
RsaI GTAC 3 cut(s) 296, 314, 480
RsaNI GTAC 3 cut(s) 295, 313, 479
RseI CAYNNNNRTG 2 cut(s) 276, 339
SacI GAGCTC 1 cut(s) 1473
SaqAI TTAA 6 cut(s) 75, 362, 522, 861, 1344, 1578
SatI GCNGC 3 cut(s) 879, 1052, 1227
Sau3AI GATC 5 cut(s) 198, 349, 564, 850, 1086
Sau96I GGNCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
SchI GAGTC 1 cut(s) 1563
ScrFI CCNGG 4 cut(s) 857, 1139, 1232, 1283
SduI GDGCHC 1 cut(s) 1473
SfaNI GCATC 7 cut(s) 165, 1034, 1211, 1321, 1396, 1429, 1539
SfcI CTRYAG 3 cut(s) 648, 1005, 1206
SfuI TTCGAA 2 cut(s) 106, 119
SinI GGWCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
SmiMI CAYNNNNRTG 2 cut(s) 276, 339
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 6 cut(s) 68, 247, 356, 503, 976, 1318
SsiI CCGC 8 cut(s) 143, 192, 232, 879, 1072, 1082, 1306, 1519
SspMI CTAG 5 cut(s) 96, 395, 590, 971, 1597
SstI GAGCTC 1 cut(s) 1473
StyD4I CCNGG 4 cut(s) 855, 1137, 1230, 1281
StyI CCWWGG 2 cut(s) 516, 1035
TaaI ACNGT 2 cut(s) 112, 299
TaiI ACGT 2 cut(s) 952, 1146
TaqI TCGA 8 cut(s) 53, 90, 106, 119, 624, 912, 952, 1423
TasI AATT 6 cut(s) 68, 247, 356, 503, 976, 1318
TatI WGTACW 2 cut(s) 312, 478
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 7 cut(s) 103, 184, 487, 548, 805, 1177, 1563
Tru1I TTAA 6 cut(s) 75, 362, 522, 861, 1344, 1578
Tru9I TTAA 6 cut(s) 75, 362, 522, 861, 1344, 1578
TscAI CASTG 3 cut(s) 117, 940, 1297
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 2 cut(s) 1051, 1226
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 5 cut(s) 80, 228, 294, 298, 955
TspRI CASTG 3 cut(s) 117, 940, 1297
VpaK11BI GGWCC 6 cut(s) 137, 299, 1032, 1069, 1106, 1325
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 1 cut(s) 503
XbaI TCTAGA 1 cut(s) 970
XceI RCATGY 1 cut(s) 672
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 5 cut(s) 96, 395, 590, 971, 1597
Zsp2I ATGCAT 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.