Rw7G018520

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000980
Physical Location & Seq
Reverse (-)
20045628 .. 20047735
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018520.1

Sequence Viewer

Length: 1077 bp
ATGGTGGGTTTGATGCTGGAATCTACAATGAAAATAACGAAGGAATGGGAGAGCCGTATACAAGAAGTTGGTGAGGATTCGAATGTTCTTGACATGAGTATTGATAAGGATTTGAAGACCCTCTCCCGAGATATCATCTCCAGGGCTTGTTTTAGCAGCTCTTACGCTCAAGGAAACCAGATTTTTGAAAAAATTTCTCTCATGCAGGAAGCTTTGTCGAAACCAAGTTTGCTTTTCGGCTTTCTAAATTTCAGGTTTCTTCCTTTTAACAATGACAAGAAGATATGGAATTTAAGGAGAGAAGTGGACGCTATGTTGCTCGAAATAGTGAGGCATCGCCAATCCGGTGGTGGTGTCCACAATGCACAAAAAGACCTACTACAAGTGCTACTAGAAAGTGCTGCTGCTGCTAACAATGGGAAGCATACACCTAAAACTGAACGTTTCGTTTTGGACAATTGTAGAACTATCTACTTTGTTGGCTCTGAGACCACTTCTCTGACTGCATCATGGACCTTGATGCTTCTTTCTTTGCACCCCGAGTGGCAGGAGCGTCTTCGAGCTGAGATTATTGGCATTTGTGGGGATCATGATCGACATGAACTCCATCGTTGCTTACAAGACATGGATACGTTAAGCAAATTAAAAATGTTAACAATGGTGATTCAAGAGAGCCTGCGTCTTTACGCACCAGCTGTCCTAACACCACGGCAAGCTCTAGCAAACATGAAATTGGGGGATCTTGATGTGCCACAAGACATGATCATATGGATAGCCACTGCTTCGTTGCACCGTGACCCCAAAAATTGGGGTCCTGATGCTAATGAGTTCAAGCCGGAGAGATTTGCAAATGGCGTAACAGAAGCATGCAAATTTCCAAATGCATATCTGCCATTTGGTTTTGGGAGTCGACTGTGTATAGGCCAGACGTTTGCGATGATGGAGCTGAAGATAGTACTCTCTCTATTACTATCGAAGTTCTCATTTTCGCTTTCACCAAACTATCACCACTCCCCTGTTTTGAAAATGGTATTGGTGCCAGAACATGGCATCAATCTCCTTGTTAGGCGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

358

Amino Acids

40.7

Weight (kDa)

7.69

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 13 - 353 3.5e-63 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1036
AccB7I CCANNNNNTGG 2 cut(s) 807, 1046
AccI GTMKAC 2 cut(s) 58, 910
AclI AACGTT 1 cut(s) 442
AclWI GGATC 2 cut(s) 594, 747
AcsI RAATTY 4 cut(s) 192, 247, 289, 872
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 1 cut(s) 957
AfiI CCNNNNNNNGG 2 cut(s) 807, 1046
AgsI TTSAA 5 cut(s) 115, 188, 668, 832, 1024
AjnI CCWGG 1 cut(s) 140
AjuI GAANNNNNNNTTGG 2 cut(s) 1016, 1048
AluBI AGCT 6 cut(s) 159, 212, 563, 695, 716, 946
AluI AGCT 6 cut(s) 159, 212, 563, 695, 716, 946
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 2 cut(s) 594, 747
Ama87I CYCGRG 2 cut(s) 126, 539
AoxI GGCC 1 cut(s) 922
ApeKI GCWGC 4 cut(s) 156, 401, 404, 407
ApoI RAATTY 4 cut(s) 192, 247, 289, 872
AspS9I GGNCC 2 cut(s) 513, 812
AsuHPI GGTGA 4 cut(s) 83, 673, 987, 998
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 2 cut(s) 126, 539
AvaII GGWCC 2 cut(s) 513, 812
BanI GGYRCC 1 cut(s) 1036
BbsI GAAGAC 2 cut(s) 122, 548
BbvI GCAGC 4 cut(s) 168, 388, 391, 394
BccI CCATC 2 cut(s) 615, 934
BceAI ACGGC 2 cut(s) 39, 725
BciT130I CCWGG 1 cut(s) 142
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 1 cut(s) 762
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 2 cut(s) 392, 719
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 4 cut(s) 157, 402, 405, 408
BlsI GCNGC 4 cut(s) 158, 403, 406, 409
BmcAI AGTACT 1 cut(s) 957
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 2 cut(s) 513, 812
BmeT110I CYCGRG 2 cut(s) 126, 539
BmgT120I GGNCC 2 cut(s) 513, 812
BmiI GGNNCC 2 cut(s) 813, 1038
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 6 cut(s) 3, 343, 510, 515, 808, 1059
BpiI GAAGAC 2 cut(s) 122, 548
BpmI CTGGAG 1 cut(s) 124
Bpu14I TTCGAA 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 141, 707
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 2 cut(s) 807, 1046
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 141, 707
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 2 cut(s) 807, 1046
BseMII CTCAG 2 cut(s) 477, 555
BseXI GCAGC 4 cut(s) 168, 388, 391, 394
BshFI GGCC 1 cut(s) 924
BshNI GGYRCC 1 cut(s) 1036
BsiHKCI CYCGRG 2 cut(s) 126, 539
BsiSI CCGG 2 cut(s) 345, 836
BslI CCNNNNNNNGG 2 cut(s) 807, 1046
BsmAI GTCTC 1 cut(s) 482
BsnI GGCC 1 cut(s) 924
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 2 cut(s) 126, 539
Bsp119I TTCGAA 1 cut(s) 80
Bsp143I GATC 4 cut(s) 586, 592, 739, 762
BspANI GGCC 1 cut(s) 924
BspCNI CTCAG 2 cut(s) 478, 556
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 813, 1038
BspPI GGATC 2 cut(s) 594, 747
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 1036
BspTNI GGTCTC 1 cut(s) 482
BssECI CCNNGG 2 cut(s) 141, 707
BssMI GATC 4 cut(s) 586, 592, 739, 762
BssNAI GTATAC 1 cut(s) 59
Bst1107I GTATAC 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 794, 915
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 3 cut(s) 677, 714, 868
BstDEI CTNAG 2 cut(s) 486, 564
BstDSI CCRYGG 1 cut(s) 707
BstKTI GATC 4 cut(s) 589, 595, 742, 765
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 4 cut(s) 586, 592, 739, 762
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 870
BstSCI CCNGG 1 cut(s) 140
BstV1I GCAGC 4 cut(s) 168, 388, 391, 394
BstV2I GAAGAC 2 cut(s) 122, 548
BstX2I RGATCY 1 cut(s) 739
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 1 cut(s) 739
BstZ17I GTATAC 1 cut(s) 59
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 1 cut(s) 924
BtgI CCRYGG 1 cut(s) 707
BtgZI GCGATG 2 cut(s) 320, 950
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 1 cut(s) 777
Cac8I GCNNGC 3 cut(s) 677, 714, 868
CciI TCATGA 1 cut(s) 589
Cfr13I GGNCC 2 cut(s) 513, 812
CseI GACGC 3 cut(s) 317, 542, 668
Csp6I GTAC 1 cut(s) 956
CviQI GTAC 1 cut(s) 956
DdeI CTNAG 2 cut(s) 486, 564
DpnI GATC 4 cut(s) 588, 594, 741, 764
DpnII GATC 4 cut(s) 586, 592, 739, 762
Eco31I GGTCTC 1 cut(s) 482
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 2 cut(s) 513, 812
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 2 cut(s) 126, 539
EcoO109I RGGNCCY 1 cut(s) 812
EcoRII CCWGG 1 cut(s) 140
EcoRV GATATC 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 886
FauNDI CATATG 1 cut(s) 767
FbaI TGATCA 1 cut(s) 762
FblI GTMKAC 2 cut(s) 58, 910
Fnu4HI GCNGC 4 cut(s) 157, 402, 405, 408
Fsp4HI GCNGC 4 cut(s) 157, 402, 405, 408
FspBI CTAG 2 cut(s) 392, 719
GluI GCNGC 4 cut(s) 157, 402, 405, 408
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 1 cut(s) 924
HapII CCGG 2 cut(s) 345, 836
HgaI GACGC 3 cut(s) 317, 542, 668
HincII GTYRAC 2 cut(s) 654, 911
HindII GTYRAC 2 cut(s) 654, 911
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 4 cut(s) 20, 77, 664, 907
HpaI GTTAAC 1 cut(s) 654
HpaII CCGG 2 cut(s) 345, 836
HphI GGTGA 4 cut(s) 83, 673, 987, 998
Hpy166II GTNNAC 5 cut(s) 59, 307, 358, 654, 911
Hpy188I TCNGA 2 cut(s) 487, 501
Hpy188III TCNNGA 6 cut(s) 89, 126, 590, 668, 743, 815
Hpy8I GTNNAC 5 cut(s) 59, 307, 358, 654, 911
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 794, 915
HpyCH4IV ACGT 3 cut(s) 442, 632, 929
HpyCH4V TGCA 8 cut(s) 205, 365, 506, 535, 790, 848, 870, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 2 cut(s) 486, 564
HpySE526I ACGT 3 cut(s) 442, 632, 929
Ksp22I TGATCA 1 cut(s) 762
KspAI GTTAAC 1 cut(s) 654
Kzo9I GATC 4 cut(s) 586, 592, 739, 762
LmnI GCTCC 2 cut(s) 550, 943
Lsp1109I GCAGC 4 cut(s) 168, 388, 391, 394
LweI GCATC 6 cut(s) 3, 343, 510, 515, 808, 1059
MaeI CTAG 2 cut(s) 392, 719
MaeII ACGT 3 cut(s) 442, 632, 929
MaeIII GTNAC 2 cut(s) 794, 856
MalI GATC 4 cut(s) 588, 594, 741, 764
MboI GATC 4 cut(s) 586, 592, 739, 762
MboII GAAGA 5 cut(s) 127, 251, 292, 548, 961
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 1 cut(s) 739
MluCI AATT 8 cut(s) 192, 247, 289, 457, 641, 731, 805, 872
MlyI GAGTC 1 cut(s) 916
MnlI CCTC 3 cut(s) 67, 131, 324
Mph1103I ATGCAT 1 cut(s) 886
MseI TTAA 5 cut(s) 267, 293, 635, 644, 653
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 2 cut(s) 345, 836
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 1 cut(s) 407
NdeI CATATG 1 cut(s) 767
NdeII GATC 4 cut(s) 586, 592, 739, 762
NlaIV GGNNCC 2 cut(s) 813, 1038
NmuCI GTSAC 1 cut(s) 794
NsiI ATGCAT 1 cut(s) 886
NspI RCATGY 1 cut(s) 870
NspV TTCGAA 1 cut(s) 80
PaeI GCATGC 1 cut(s) 870
PagI TCATGA 1 cut(s) 589
PfeI GAWTC 3 cut(s) 20, 77, 664
PflMI CCANNNNNTGG 2 cut(s) 807, 1046
PkrI GCNGC 4 cut(s) 158, 403, 406, 409
PleI GAGTC 1 cut(s) 915
PpsI GAGTC 1 cut(s) 915
PpuMI RGGWCCY 1 cut(s) 812
Psp1406I AACGTT 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 813, 1038
PspPI GGNCC 2 cut(s) 513, 812
PspPPI RGGWCCY 1 cut(s) 812
PsuI RGATCY 1 cut(s) 739
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 1 cut(s) 957
RsaNI GTAC 1 cut(s) 956
SalI GTCGAC 1 cut(s) 909
SaqAI TTAA 5 cut(s) 267, 293, 635, 644, 653
SatI GCNGC 4 cut(s) 157, 402, 405, 408
Sau3AI GATC 4 cut(s) 586, 592, 739, 762
Sau96I GGNCC 2 cut(s) 513, 812
ScaI AGTACT 1 cut(s) 957
SchI GAGTC 1 cut(s) 916
ScrFI CCNGG 1 cut(s) 142
SfaNI GCATC 6 cut(s) 3, 343, 510, 515, 808, 1059
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 2 cut(s) 513, 812
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
SphI GCATGC 1 cut(s) 870
Sse9I AATT 8 cut(s) 192, 247, 289, 457, 641, 731, 805, 872
SspMI CTAG 2 cut(s) 392, 719
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 2 cut(s) 794, 915
TaiI ACGT 3 cut(s) 445, 635, 932
TaqI TCGA 7 cut(s) 80, 218, 321, 559, 595, 910, 974
TasI AATT 8 cut(s) 192, 247, 289, 457, 641, 731, 805, 872
TatI WGTACW 1 cut(s) 955
TfiI GAWTC 3 cut(s) 20, 77, 664
Tru1I TTAA 5 cut(s) 267, 293, 635, 644, 653
Tru9I TTAA 5 cut(s) 267, 293, 635, 644, 653
TscAI CASTG 1 cut(s) 784
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 4 cut(s) 156, 401, 404, 407
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 3 cut(s) 44, 615, 743
TspRI CASTG 1 cut(s) 784
Van91I CCANNNNNTGG 2 cut(s) 807, 1046
VpaK11BI GGWCC 2 cut(s) 513, 812
XapI RAATTY 4 cut(s) 192, 247, 289, 872
XceI RCATGY 1 cut(s) 870
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmiI GTMKAC 2 cut(s) 58, 910
XspI CTAG 2 cut(s) 392, 719
ZrmI AGTACT 1 cut(s) 957
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.