RLG00000025065

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000980
Physical Location & Seq
Reverse (-)
41726731 .. 41730179
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025065

Sequence Viewer

Length: 1629 bp
ATGGAAGTGAATGCATTACCGATATGGTGGTCAGTTTTGTTAATCGGTGTATCAAGCTTGGTAATCAAATTGCTTAACAAGTTGTGGTTCGAGCCTAGAAGGATTCGAACAGTGCTTTCGAAGCAAGGCATTAGAGGACCACGACCCACCTTCCCTTTCGGCAATGTCCCAGAGATGCAGAAGATTCAATCAACCATGATCAACAACACGCATGAAGAAATGGATGGTCAACATCTCAACCACAATTGGCTTCCCTCTGTATTTCCATATATTCATAAATGGGCAAAAGAGTATGGTCCAATATACATGTACTCCACAGGGATCAATCAACACCTATATGTGAGCGATCCGAAGTTGCTAATGGAGATAAAACAGCACAACTCCCTGGATCTGGGTAAACCTACATATCTGGGTAAACCACTCCAGCCCTTGTTAGGTGATGGCCTTCTCCGAACTAATGGAAAACAGTGGGCCGTCCAGAGGAAACTTCTTGCTCCTGAATTCTTCCTTGGCAAAGTTAAGGGCATGGTGGGTGTGATGGAGGAATCTACAACAGCAATGATCAAGAGATGGGATTGTCGTATACTAGAAAGCAAATCAGGCATTGTGGACATTGAAATTGATGACGATTTGAAGACACTATCAGCCGATATCATATCAAGAGCATGTTTTGGCAGCTCTTACTCCTTTGGAAATCAGATTTTTGAAAAGATTGCTGACATGGTGGAAGTTTATGGCAAACCAAGTTTACAATTTGGCTTTCTTAATTTCATTAGCCCACGTTTCTCTAATTTCAAACGTTGTTCCTGCAGGTTTCTTCCTAGCAAGAAGTTATGGAATCTAAGGAAAGAAGTGGACTCTATGTTGCTCAAGTTGGTGACGGATCGCCAGGTTGAAAGACAATCGAGTGGTGCAACAGAAAATGACCTGTTACAAATGCTACTCGATATTGCTTCTACCAACACTGACATTCCTGCGCGTAGAAGAGAAACTTTCGTTCTAGACAATTGTAGAAATATCTACTTTGCAAGCTCTGCAACCACTTCTCTTACGGAATCATGGACCTTGATGCTTCTGTCATTGCACCCGGAATGGCAGGACCGCATTCGTGTGGAGATCATTGAGGTTTGTGGGGTTCTGGATGAACTCCATCATTCCTTGCACGACATGGATATGTTGCGAAAGTTCAAAGTGCTGACTATGGTGATTCAAGAGAGTATGCGTCTTTATCCATCTGGAGTCCTAATTGCAAGGGCTATTCTGGCAAATGTGAAGTTGGGCGACCTTGATGTGCCCAAAGGCACCAACATCCATGTATGCAGTGCTTCATTACACTGCGACCCAGAAAATTGGGGTCCAGATGCTGATGAGTTTAAGCCAGAGAGGTTTGCAAATGGGATATCTAATGCCTGCAAGCATCCCCAAGCCTATGTGCCATTTGGTTTTGGAACTCGCGTGTGCATAGGACAGACTTTTGCTATGACACAGCTGAAGATAGTGCTCTCTCTAATCTTGTCCAAGTTCTCATTTTCCCTTTCTCCAAACTATCGCCATTCACCTGTTCACAAAATGATACTGATGCCTAAACATGGAATCAGACTCCTTGTTGAGCCTGTGCAAACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

543

Amino Acids

61.75

Weight (kDa)

8.92

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 89 - 535 3.6e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 801
AccB1I GGYRCC 1 cut(s) 1301
AccI GTMKAC 1 cut(s) 583
AccII CGCG 2 cut(s) 979, 1455
AciI CCGC 1 cut(s) 1102
AclI AACGTT 1 cut(s) 799
AclWI GGATC 4 cut(s) 329, 341, 396, 891
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 1511
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1589
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 8 cut(s) 188, 617, 634, 707, 796, 896, 1189, 1211
AjnI CCWGG 2 cut(s) 384, 888
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AluBI AGCT 5 cut(s) 57, 678, 1032, 1489, 1626
AluI AGCT 5 cut(s) 57, 678, 1032, 1489, 1626
Alw21I GWGCWC 1 cut(s) 1503
AlwI GGATC 4 cut(s) 329, 341, 396, 891
AlwNI CAGNNNCTG 1 cut(s) 1364
AoxI GGCC 2 cut(s) 442, 471
ApeKI GCWGC 1 cut(s) 675
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 1 cut(s) 979
AspS9I GGNCC 6 cut(s) 137, 296, 471, 1062, 1099, 1355
AsuC2I CCSGG 1 cut(s) 1088
AsuHPI GGTGA 4 cut(s) 449, 889, 1216, 1548
AsuII TTCGAA 2 cut(s) 106, 119
AvaII GGWCC 5 cut(s) 137, 296, 1062, 1099, 1355
BaeGI GKGCMC 1 cut(s) 1296
BanI GGYRCC 1 cut(s) 1301
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 1503
BbvI GCAGC 1 cut(s) 687
BccI CCATC 6 cut(s) 218, 434, 532, 564, 1158, 1240
BceAI ACGGC 1 cut(s) 458
BciT130I CCWGG 2 cut(s) 386, 890
BclI TGATCA 2 cut(s) 198, 561
BcnI CCSGG 1 cut(s) 1088
BfaI CTAG 5 cut(s) 96, 587, 822, 1001, 1627
BfmI CTRYAG 1 cut(s) 808
BfuAI ACCTGC 1 cut(s) 801
BisI GCNGC 1 cut(s) 676
BlsI GCNGC 1 cut(s) 677
Bme1390I CCNGG 3 cut(s) 386, 890, 1088
Bme18I GGWCC 5 cut(s) 137, 296, 1062, 1099, 1355
BmgT120I GGNCC 6 cut(s) 137, 296, 471, 1062, 1099, 1355
BmiI GGNNCC 2 cut(s) 1303, 1356
BmrFI CCNGG 3 cut(s) 386, 890, 1088
BmsI GCATC 5 cut(s) 165, 1059, 1351, 1426, 1569
BpiI GAAGAC 1 cut(s) 641
BpmI CTGGAG 2 cut(s) 407, 1257
Bpu14I TTCGAA 2 cut(s) 106, 119
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 1 cut(s) 1088
BsaJI CCNNGG 2 cut(s) 384, 508
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1589
Bse3DI GCAATG 3 cut(s) 169, 564, 1079
BseBI CCWGG 2 cut(s) 386, 890
BseDI CCNNGG 2 cut(s) 384, 508
BseGI GGATG 4 cut(s) 229, 1147, 1308, 1417
BseLI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1589
BseMI GCAATG 3 cut(s) 169, 564, 1079
BseSI GKGCMC 1 cut(s) 1296
BseXI GCAGC 1 cut(s) 687
Bsh1236I CGCG 2 cut(s) 979, 1455
BshFI GGCC 2 cut(s) 444, 473
BshNI GGYRCC 1 cut(s) 1301
BsiHKAI GWGCWC 1 cut(s) 1503
BsiSI CCGG 1 cut(s) 1088
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1589
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 2 cut(s) 16, 1104
BsnI GGCC 2 cut(s) 444, 473
Bsp119I TTCGAA 2 cut(s) 106, 119
Bsp1286I GDGCHC 2 cut(s) 1296, 1503
Bsp143I GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
BspACI CCGC 1 cut(s) 1102
BspANI GGCC 2 cut(s) 444, 473
BspFNI CGCG 2 cut(s) 979, 1455
BspLI GGNNCC 2 cut(s) 1303, 1356
BspMAI CTGCAG 1 cut(s) 812
BspMI ACCTGC 1 cut(s) 801
BspPI GGATC 4 cut(s) 329, 341, 396, 891
BspT104I TTCGAA 2 cut(s) 106, 119
BspT107I GGYRCC 1 cut(s) 1301
BsrDI GCAATG 3 cut(s) 169, 564, 1079
BssECI CCNNGG 2 cut(s) 384, 508
BssMI GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
BssNAI GTATAC 1 cut(s) 584
BssT1I CCWWGG 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 386, 890
Bst4CI ACNGT 2 cut(s) 112, 468
Bst6I CTCTTC 1 cut(s) 979
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstBI TTCGAA 2 cut(s) 106, 119
BstC8I GCNNGC 3 cut(s) 1030, 1411, 1415
BstDEI CTNAG 1 cut(s) 842
BstF5I GGATG 4 cut(s) 229, 1147, 1308, 1417
BstFNI CGCG 2 cut(s) 979, 1455
BstHHI GCGC 1 cut(s) 979
BstKTI GATC 7 cut(s) 201, 324, 349, 391, 564, 886, 1119
BstMBI GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
BstMWI GCNNNNNNNGC 4 cut(s) 121, 600, 1034, 1262
BstNI CCWGG 2 cut(s) 386, 890
BstNSI RCATGY 2 cut(s) 310, 669
BstSCI CCNGG 3 cut(s) 384, 888, 1086
BstSFI CTRYAG 1 cut(s) 808
BstSLI GKGCMC 1 cut(s) 1296
BstUI CGCG 2 cut(s) 979, 1455
BstV1I GCAGC 1 cut(s) 687
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 1350
BstYI RGATCY 1 cut(s) 388
BstZ17I GTATAC 1 cut(s) 584
BsuRI GGCC 2 cut(s) 444, 473
BtsCI GGATG 4 cut(s) 229, 1147, 1308, 1417
BtsI GCAGTG 2 cut(s) 1327, 1333
BtsIMutI CAGTG 5 cut(s) 117, 473, 963, 1327, 1333
BveI ACCTGC 1 cut(s) 801
Cac8I GCNNGC 3 cut(s) 1030, 1411, 1415
CaiI CAGNNNCTG 1 cut(s) 1364
CfoI GCGC 1 cut(s) 979
Cfr13I GGNCC 6 cut(s) 137, 296, 471, 1062, 1099, 1355
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 1 cut(s) 310
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 1 cut(s) 842
DpnI GATC 7 cut(s) 200, 323, 348, 390, 563, 885, 1118
DpnII GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
Eam1104I CTCTTC 1 cut(s) 979
EarI CTCTTC 1 cut(s) 979
Eco130I CCWWGG 1 cut(s) 508
Eco32I GATATC 2 cut(s) 652, 1401
Eco47I GGWCC 5 cut(s) 137, 296, 1062, 1099, 1355
Eco57I CTGAAG 1 cut(s) 1511
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 2 cut(s) 384, 888
EcoRV GATATC 2 cut(s) 652, 1401
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 508
FalI AAGNNNNNCTT 2 cut(s) 976, 1008
FaqI GGGAC 1 cut(s) 152
FbaI TGATCA 2 cut(s) 198, 561
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 676
FokI GGATG 4 cut(s) 236, 1154, 1295, 1404
Fsp4HI GCNGC 1 cut(s) 676
FspBI CTAG 5 cut(s) 96, 587, 822, 1001, 1627
GlaI GCGC 1 cut(s) 978
GluI GCNGC 1 cut(s) 676
GsuI CTGGAG 2 cut(s) 407, 1257
HaeIII GGCC 2 cut(s) 444, 473
HapII CCGG 1 cut(s) 1088
HgaI GACGC 1 cut(s) 1211
HhaI GCGC 1 cut(s) 979
Hin6I GCGC 1 cut(s) 977
HinP1I GCGC 1 cut(s) 977
HincII GTYRAC 1 cut(s) 230
HindII GTYRAC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 55
HpaII CCGG 1 cut(s) 1088
HphI GGTGA 4 cut(s) 449, 889, 1216, 1548
Hpy166II GTNNAC 8 cut(s) 230, 398, 416, 584, 610, 749, 856, 1564
Hpy188I TCNGA 4 cut(s) 351, 452, 699, 1598
Hpy188III TCNNGA 9 cut(s) 478, 497, 565, 660, 1001, 1139, 1211, 1236, 1358
Hpy8I GTNNAC 8 cut(s) 230, 398, 416, 584, 610, 749, 856, 1564
HpyAV CCTTC 3 cut(s) 93, 160, 455
HpyCH4III ACNGT 2 cut(s) 112, 468
HpyCH4IV ACGT 2 cut(s) 781, 799
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 600, 1034, 1262
HpyF3I CTNAG 1 cut(s) 842
HpySE526I ACGT 2 cut(s) 781, 799
HspAI GCGC 1 cut(s) 977
Ksp22I TGATCA 2 cut(s) 198, 561
Kzo9I GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
LmnI GCTCC 1 cut(s) 499
Lsp1109I GCAGC 1 cut(s) 687
LweI GCATC 5 cut(s) 165, 1059, 1351, 1426, 1569
MaeI CTAG 5 cut(s) 96, 587, 822, 1001, 1627
MaeII ACGT 2 cut(s) 781, 799
MaeIII GTNAC 2 cut(s) 877, 930
MalI GATC 7 cut(s) 200, 323, 348, 390, 563, 885, 1118
MboI GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
MboII GAAGA 7 cut(s) 193, 227, 496, 646, 809, 996, 1504
MfeI CAATTG 2 cut(s) 244, 1006
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 1296, 1503
MlyI GAGTC 3 cut(s) 851, 1248, 1593
MnlI CCTC 6 cut(s) 128, 265, 474, 535, 1117, 1377
Mph1103I ATGCAT 1 cut(s) 16
MseI TTAA 5 cut(s) 41, 75, 519, 765, 1374
MslI CAYNNNNRTG 3 cut(s) 336, 1109, 1172
MspA1I CMGCKG 1 cut(s) 1489
MspI CCGG 1 cut(s) 1088
MspR9I CCNGG 3 cut(s) 386, 890, 1088
MunI CAATTG 2 cut(s) 244, 1006
Mva1269I GAATGC 2 cut(s) 16, 1104
MvaI CCWGG 2 cut(s) 386, 890
MvnI CGCG 2 cut(s) 979, 1455
MwoI GCNNNNNNNGC 4 cut(s) 121, 600, 1034, 1262
NciI CCSGG 1 cut(s) 1088
NdeII GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
NlaIV GGNNCC 2 cut(s) 1303, 1356
NmuCI GTSAC 1 cut(s) 877
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 2 cut(s) 310, 669
NspV TTCGAA 2 cut(s) 106, 119
PciI ACATGT 1 cut(s) 306
PctI GAATGC 2 cut(s) 16, 1104
PfeI GAWTC 7 cut(s) 103, 184, 545, 838, 1055, 1207, 1593
PkrI GCNGC 1 cut(s) 677
PleI GAGTC 3 cut(s) 851, 1247, 1593
PpsI GAGTC 3 cut(s) 851, 1247, 1593
PscI ACATGT 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 799
Psp6I CCWGG 2 cut(s) 384, 888
PspGI CCWGG 2 cut(s) 384, 888
PspN4I GGNNCC 2 cut(s) 1303, 1356
PspPI GGNCC 6 cut(s) 137, 296, 471, 1062, 1099, 1355
PstI CTGCAG 1 cut(s) 812
PstNI CAGNNNCTG 1 cut(s) 1364
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 1489
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 3 cut(s) 336, 1109, 1172
SaqAI TTAA 5 cut(s) 41, 75, 519, 765, 1374
SatI GCNGC 1 cut(s) 676
Sau3AI GATC 7 cut(s) 198, 321, 346, 388, 561, 883, 1116
Sau96I GGNCC 6 cut(s) 137, 296, 471, 1062, 1099, 1355
SbfI CCTGCAGG 1 cut(s) 812
SchI GAGTC 3 cut(s) 851, 1248, 1593
ScrFI CCNGG 3 cut(s) 386, 890, 1088
SdaI CCTGCAGG 1 cut(s) 812
SduI GDGCHC 2 cut(s) 1296, 1503
SfaNI GCATC 5 cut(s) 165, 1059, 1351, 1426, 1569
SfcI CTRYAG 1 cut(s) 808
SfuI TTCGAA 2 cut(s) 106, 119
SinI GGWCC 5 cut(s) 137, 296, 1062, 1099, 1355
SmiMI CAYNNNNRTG 3 cut(s) 336, 1109, 1172
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
Sse8387I CCTGCAGG 1 cut(s) 812
SsiI CCGC 1 cut(s) 1102
SspMI CTAG 5 cut(s) 96, 587, 822, 1001, 1627
StyD4I CCNGG 3 cut(s) 384, 888, 1086
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 2 cut(s) 112, 468
TaiI ACGT 2 cut(s) 784, 802
TaqI TCGA 5 cut(s) 90, 106, 119, 905, 945
TatI WGTACW 1 cut(s) 309
TfiI GAWTC 7 cut(s) 103, 184, 545, 838, 1055, 1207, 1593
Tru1I TTAA 5 cut(s) 41, 75, 519, 765, 1374
Tru9I TTAA 5 cut(s) 41, 75, 519, 765, 1374
TscAI CASTG 5 cut(s) 117, 473, 970, 1327, 1340
TseFI GTSAC 1 cut(s) 877
TseI GCWGC 1 cut(s) 675
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 5 cut(s) 228, 263, 760, 1158, 1317
TspGWI ACGGA 2 cut(s) 896, 1067
TspRI CASTG 5 cut(s) 117, 473, 970, 1327, 1340
VpaK11BI GGWCC 5 cut(s) 137, 296, 1062, 1099, 1355
XapI RAATTY 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 2 cut(s) 310, 669
XmiI GTMKAC 1 cut(s) 583
XspI CTAG 5 cut(s) 96, 587, 822, 1001, 1627
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.