Rh7BG211500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000980
Physical Location & Seq
Reverse (-)
17859718 .. 17861969
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG211500.1

Sequence Viewer

Length: 1296 bp
ATGTACTCCACAGGGAGCAAGCAACACCTTTATGTGAGTGATCCAAATTTGTTAAGGGGGATCAAACAGCATAACACCTTGGATCTGGGTAAACCTACGTACCTGGGTAAGCCACTCCAACCCTTGCTAGGTGACGGAATTGTCAGGTCCAACGGACAACACTGGGCCAACCAGAAAAAAATCATTGCTCCGGAATTCTTCCTTAACAAGGTTATGGGCATGGTGGGTTTGATGCTGGAATCTACAATGAAAATAATGAAGGAATGGGAGAGCCGTATACAAGAAGTTGGTGAGGATTCGAATGTTCTTGACATGAGTATTGATAAGGATTTGAAGACCCTCTCCCGAGATATCATCTCCAGGGCTTGTTTTAGCAGCTCTTACGCTCAAGGAAACCAGATTTTTGAAAAGATTTCTCTCATGCAGGAAGCTTTGTCGAAACCAAGTTTGCTTTTCGGCTTTCTAAATTTCAGGTTTCTTCCTTTTAACAATGACAAGAAGATATGGAATTTAAGGAGAGAAGTGGACGCTATGTTGCTCGAAATAGTGAGGCATCGCCAATCCGGTGGTGGTGTCCACAATGCACAAAAAGACCTACTACAAGTGCTACTAGAAAGTGCTGCTGCTGCTAACAATGGGAAGCATACACCTAAAACTGAACGTTTCGTTTTGGACAATTGTAGAACTATCTACTTTGTTGGCTCTGAGACCACTTCTCTGACTGCATCATGGACCTTGATGCTTCTTTCTTTGCACCCCGAGTGGCAGGAGCGTCTTCGAGCTGAGATTATTGGCATTTGTGGGGATCATGATCGACATGAACTCCATCGTTGCTTACAAGACATGGATACGTTAAGCAAATTAAAAATGTTAACAATGGTGATTCAAGAGAGCCTGCGTCTTTACGCACCAGCTGTCCTAACACCACGGCAAGCTCTAGCAAACATGAAATTGGGGGATCTTGATGTGCCACAAGACATGATCATATGGATAGCCATTGCATCGTTGCACCGTGACCCAAAAAATTGGGGTCCAGATGCTAATGAGTTCAAGCCGGAGAGATTTGCAAATGGCGTAACAGAAGCATGCAAATTTCCAAATGCATATCTGCCATTTGGTTTTGGGAGTCGACTGTGTATAGGCCAGACGTTTGCGATGATGGAGCTGAAGATAGTACTCTCTCTATTACTATCGAAGTTCTCATTTTCGCTTTCACCAAACTATCACCACTCCCCCGTTTTGAAATTGGTATTGGTGCCAGAACATGGCATCAATCTCCTTGTTAGGCGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

431

Amino Acids

48.88

Weight (kDa)

8.88

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 426 3.9e-72 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 1255
AccB7I CCANNNNNTGG 1 cut(s) 1265
AccI GTMKAC 2 cut(s) 277, 1129
AccIII TCCGGA 1 cut(s) 190
AclI AACGTT 1 cut(s) 661
AclWI GGATC 5 cut(s) 35, 68, 90, 813, 966
AcsI RAATTY 5 cut(s) 46, 194, 466, 508, 1091
AcuI CTGAAG 1 cut(s) 1187
AfaI GTAC 3 cut(s) 5, 101, 1176
AfiI CCNNNNNNNGG 3 cut(s) 128, 208, 1265
AgsI TTSAA 5 cut(s) 334, 407, 887, 1051, 1243
AjnI CCWGG 2 cut(s) 102, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 1235, 1267
AluBI AGCT 6 cut(s) 378, 431, 782, 914, 935, 1165
AluI AGCT 6 cut(s) 378, 431, 782, 914, 935, 1165
Alw26I GTCTC 1 cut(s) 701
AlwI GGATC 5 cut(s) 35, 68, 90, 813, 966
Ama87I CYCGRG 2 cut(s) 345, 758
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 2 cut(s) 165, 1141
ApeKI GCWGC 4 cut(s) 375, 620, 623, 626
ApoI RAATTY 5 cut(s) 46, 194, 466, 508, 1091
AspS9I GGNCC 4 cut(s) 147, 165, 732, 1031
AsuHPI GGTGA 5 cut(s) 143, 302, 892, 1206, 1217
AsuII TTCGAA 1 cut(s) 299
AvaI CYCGRG 2 cut(s) 345, 758
AvaII GGWCC 3 cut(s) 147, 732, 1031
BaeI ACNNNNGTAYC 2 cut(s) 83, 116
BanI GGYRCC 1 cut(s) 1255
BbsI GAAGAC 2 cut(s) 341, 767
BbvI GCAGC 4 cut(s) 387, 607, 610, 613
BccI CCATC 2 cut(s) 834, 1153
BceAI ACGGC 2 cut(s) 258, 944
BciT130I CCWGG 2 cut(s) 104, 361
BciVI GTATCC 1 cut(s) 841
BclI TGATCA 1 cut(s) 981
BcoDI GTCTC 1 cut(s) 701
BfaI CTAG 3 cut(s) 128, 611, 938
BfuI GTATCC 1 cut(s) 841
BisI GCNGC 4 cut(s) 376, 621, 624, 627
BlsI GCNGC 4 cut(s) 377, 622, 625, 628
BmcAI AGTACT 1 cut(s) 1176
Bme1390I CCNGG 2 cut(s) 104, 361
Bme18I GGWCC 3 cut(s) 147, 732, 1031
BmeT110I CYCGRG 2 cut(s) 345, 758
BmgT120I GGNCC 4 cut(s) 147, 165, 732, 1031
BmiI GGNNCC 2 cut(s) 1032, 1257
BmrFI CCNGG 2 cut(s) 104, 361
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 7 cut(s) 222, 562, 729, 734, 1010, 1027, 1278
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 341, 767
BpmI CTGGAG 1 cut(s) 343
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 372
BsaAI YACGTR 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 810
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 4 cut(s) 78, 103, 360, 926
BsaWI WCCGGW 2 cut(s) 190, 563
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 208, 1265
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 2 cut(s) 183, 996
Bse8I GATNNNNATC 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 2 cut(s) 104, 361
BseDI CCNNGG 4 cut(s) 78, 103, 360, 926
BseJI GATNNNNATC 1 cut(s) 810
BseLI CCNNNNNNNGG 3 cut(s) 128, 208, 1265
BseMI GCAATG 2 cut(s) 183, 996
BseMII CTCAG 2 cut(s) 696, 774
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 4 cut(s) 387, 607, 610, 613
BshFI GGCC 2 cut(s) 167, 1143
BshNI GGYRCC 1 cut(s) 1255
BsiHKCI CYCGRG 2 cut(s) 345, 758
BsiSI CCGG 3 cut(s) 191, 564, 1055
BslI CCNNNNNNNGG 3 cut(s) 128, 208, 1265
BsmAI GTCTC 1 cut(s) 701
BsnI GGCC 2 cut(s) 167, 1143
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 2 cut(s) 345, 758
Bsp119I TTCGAA 1 cut(s) 299
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
BspANI GGCC 2 cut(s) 167, 1143
BspCNI CTCAG 2 cut(s) 697, 775
BspEI TCCGGA 1 cut(s) 190
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 2 cut(s) 1032, 1257
BspPI GGATC 5 cut(s) 35, 68, 90, 813, 966
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 1255
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 183, 996
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 78, 103, 360, 926
BssMI GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
BssNAI GTATAC 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 104, 361
Bst4CI ACNGT 2 cut(s) 1013, 1134
BstBAI YACGTR 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 4 cut(s) 20, 896, 933, 1087
BstDEI CTNAG 2 cut(s) 705, 783
BstDSI CCRYGG 1 cut(s) 926
BstENI CCTNNNNNAGG 1 cut(s) 206
BstKTI GATC 7 cut(s) 43, 63, 85, 808, 814, 961, 984
BstMAI GTCTC 1 cut(s) 701
BstMBI GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNI CCWGG 2 cut(s) 104, 361
BstNSI RCATGY 1 cut(s) 1089
BstSCI CCNGG 2 cut(s) 102, 359
BstSNI TACGTA 1 cut(s) 99
BstV1I GCAGC 4 cut(s) 387, 607, 610, 613
BstV2I GAAGAC 2 cut(s) 341, 767
BstX2I RGATCY 2 cut(s) 82, 958
BstXI CCANNNNNNTGG 2 cut(s) 566, 1026
BstYI RGATCY 2 cut(s) 82, 958
BstZ17I GTATAC 1 cut(s) 278
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 2 cut(s) 167, 1143
BtgI CCRYGG 1 cut(s) 926
BtgZI GCGATG 2 cut(s) 539, 1169
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 4 cut(s) 20, 896, 933, 1087
CciI TCATGA 1 cut(s) 808
Cfr13I GGNCC 4 cut(s) 147, 165, 732, 1031
CseI GACGC 3 cut(s) 536, 761, 887
Csp6I GTAC 3 cut(s) 4, 100, 1175
CviQI GTAC 3 cut(s) 4, 100, 1175
DdeI CTNAG 2 cut(s) 705, 783
DpnI GATC 7 cut(s) 42, 62, 84, 807, 813, 960, 983
DpnII GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
Eco105I TACGTA 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 701
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 3 cut(s) 147, 732, 1031
Eco57I CTGAAG 1 cut(s) 1187
Eco88I CYCGRG 2 cut(s) 345, 758
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 102, 359
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 1105
ErhI CCWWGG 1 cut(s) 78
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 1 cut(s) 981
FblI GTMKAC 2 cut(s) 277, 1129
Fnu4HI GCNGC 4 cut(s) 376, 621, 624, 627
Fsp4HI GCNGC 4 cut(s) 376, 621, 624, 627
FspBI CTAG 3 cut(s) 128, 611, 938
GluI GCNGC 4 cut(s) 376, 621, 624, 627
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 2 cut(s) 167, 1143
HapII CCGG 3 cut(s) 191, 564, 1055
HgaI GACGC 3 cut(s) 536, 761, 887
HincII GTYRAC 2 cut(s) 873, 1130
HindII GTYRAC 2 cut(s) 873, 1130
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 4 cut(s) 239, 296, 883, 1126
HpaI GTTAAC 1 cut(s) 873
HpaII CCGG 3 cut(s) 191, 564, 1055
HphI GGTGA 5 cut(s) 143, 302, 892, 1206, 1217
Hpy166II GTNNAC 6 cut(s) 92, 278, 526, 577, 873, 1130
Hpy188I TCNGA 2 cut(s) 706, 720
Hpy188III TCNNGA 7 cut(s) 191, 308, 345, 809, 887, 962, 1034
Hpy8I GTNNAC 6 cut(s) 92, 278, 526, 577, 873, 1130
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 1013, 1134
HpyCH4IV ACGT 4 cut(s) 98, 661, 851, 1148
HpyCH4V TGCA 9 cut(s) 424, 584, 725, 754, 1001, 1009, 1067, 1089, 1103
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpyF3I CTNAG 2 cut(s) 705, 783
HpySE526I ACGT 4 cut(s) 98, 661, 851, 1148
Kpn2I TCCGGA 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 981
KspAI GTTAAC 1 cut(s) 873
Kzo9I GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
LmnI GCTCC 4 cut(s) 15, 193, 769, 1162
Lsp1109I GCAGC 4 cut(s) 387, 607, 610, 613
LweI GCATC 7 cut(s) 222, 562, 729, 734, 1010, 1027, 1278
MaeI CTAG 3 cut(s) 128, 611, 938
MaeII ACGT 4 cut(s) 98, 661, 851, 1148
MaeIII GTNAC 3 cut(s) 131, 1013, 1075
MalI GATC 7 cut(s) 42, 62, 84, 807, 813, 960, 983
MboI GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
MboII GAAGA 6 cut(s) 190, 346, 470, 511, 767, 1180
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 2 cut(s) 82, 958
MlyI GAGTC 1 cut(s) 1135
MmeI TCCRAC 2 cut(s) 142, 174
MnlI CCTC 3 cut(s) 286, 350, 543
Mph1103I ATGCAT 1 cut(s) 1105
MroI TCCGGA 1 cut(s) 190
MseI TTAA 7 cut(s) 53, 204, 486, 512, 854, 863, 872
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 3 cut(s) 191, 564, 1055
MspR9I CCNGG 2 cut(s) 104, 361
MunI CAATTG 1 cut(s) 676
MvaI CCWGG 2 cut(s) 104, 361
MwoI GCNNNNNNNGC 1 cut(s) 626
NdeI CATATG 1 cut(s) 986
NdeII GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
NlaIV GGNNCC 2 cut(s) 1032, 1257
NmuCI GTSAC 2 cut(s) 131, 1013
NsiI ATGCAT 1 cut(s) 1105
NspI RCATGY 1 cut(s) 1089
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 1 cut(s) 1089
PagI TCATGA 1 cut(s) 808
PfeI GAWTC 3 cut(s) 239, 296, 883
PflMI CCANNNNNTGG 1 cut(s) 1265
PkrI GCNGC 4 cut(s) 377, 622, 625, 628
PleI GAGTC 1 cut(s) 1134
PpsI GAGTC 1 cut(s) 1134
Ppu21I YACGTR 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 661
Psp6I CCWGG 2 cut(s) 102, 359
PspGI CCWGG 2 cut(s) 102, 359
PspN4I GGNNCC 2 cut(s) 1032, 1257
PspPI GGNCC 4 cut(s) 147, 165, 732, 1031
PsuI RGATCY 2 cut(s) 82, 958
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 3 cut(s) 5, 101, 1176
RsaNI GTAC 3 cut(s) 4, 100, 1175
RseI CAYNNNNRTG 1 cut(s) 30
SalI GTCGAC 1 cut(s) 1128
SaqAI TTAA 7 cut(s) 53, 204, 486, 512, 854, 863, 872
SatI GCNGC 4 cut(s) 376, 621, 624, 627
Sau3AI GATC 7 cut(s) 40, 60, 82, 805, 811, 958, 981
Sau96I GGNCC 4 cut(s) 147, 165, 732, 1031
ScaI AGTACT 1 cut(s) 1176
SchI GAGTC 1 cut(s) 1135
ScrFI CCNGG 2 cut(s) 104, 361
SfaNI GCATC 7 cut(s) 222, 562, 729, 734, 1010, 1027, 1278
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 3 cut(s) 147, 732, 1031
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
SnaBI TACGTA 1 cut(s) 99
SphI GCATGC 1 cut(s) 1089
SspMI CTAG 3 cut(s) 128, 611, 938
StyD4I CCNGG 2 cut(s) 102, 359
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 1013, 1134
TaiI ACGT 4 cut(s) 101, 664, 854, 1151
TaqI TCGA 7 cut(s) 299, 437, 540, 778, 814, 1129, 1193
TatI WGTACW 2 cut(s) 3, 1174
TfiI GAWTC 3 cut(s) 239, 296, 883
Tru1I TTAA 7 cut(s) 53, 204, 486, 512, 854, 863, 872
Tru9I TTAA 7 cut(s) 53, 204, 486, 512, 854, 863, 872
TscAI CASTG 1 cut(s) 167
TseFI GTSAC 2 cut(s) 131, 1013
TseI GCWGC 4 cut(s) 375, 620, 623, 626
Tsp45I GTSAC 2 cut(s) 131, 1013
TspDTI ATGAA 4 cut(s) 263, 272, 834, 962
TspGWI ACGGA 2 cut(s) 150, 168
TspRI CASTG 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 1265
VpaK11BI GGWCC 3 cut(s) 147, 732, 1031
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 5 cut(s) 46, 194, 466, 508, 1091
XceI RCATGY 1 cut(s) 1089
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmiI GTMKAC 2 cut(s) 277, 1129
XspI CTAG 3 cut(s) 128, 611, 938
ZrmI AGTACT 1 cut(s) 1176
Zsp2I ATGCAT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.