Rh2DG322800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000980
Physical Location & Seq
Forward (+)
40412447 .. 40413441
995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG322800.1

Sequence Viewer

Length: 849 bp
ATGTTTAAATTGTACAGACTCTTCCCCACAGTGAATAGCAAGTTGATAAAGAGTTTAAAGAAAAAGGTGGACGCTTTGATACTTAAATTAGCAAGGGATCGTCAGGTTGAAATGCAAAGGAATGGTGCATCTCAAAAGGACCTATTACAAATTATACTTGAAAGTGCTGCAAATAGTACACACATGCCTGCCCAACTGAAATCAGATAGTTTTATTTTGGACGCTTGTAAAAGTATCTACTTTGCAGGCGCCGAGAGCACTGCTACTGCTCTAGCCTGGACCTTGATGCTTCTGTCACTGCACCAAGAATGGCAAGAACGTATTCGCGCTGAGATTGTTGAAGTGTGTGGGGATCGTGACCTCCATCAATGCTTGCAACAGATGGATACATTAACCAAACTCAAAACGCTGACAATAGTCATTCAAGAGAGTCTGCGTCTTTATCCACCGGCCCCAATAACAGCCAAGCATGCTTTAGCAGACATGAAACTAGGGGACCTTGATGTGCCAGGAGGCACCAACATATGGATCTTCACACACAGATTGCAGTGCGACCCGGAGAATTGGGGGCCAGATGCTAATGAGTTTAAGCCAGAGAGATTTGCAGATGGGTTCTCCAAATCATGCAAGTATGCCCAAGCATATGTGCCATTTGGTTTCGGGACTCGAATTTGCATAGGACAGGACTTTGCTATGACACAAATAAAGATAGTACTCTCCGTAATCCTTTCCAAATTCTCATTTTCTATTTCTCCGAATTATCACCATTCTCCTGTGGATCAAATGTTACTATTGCCTAGACATGGAATTAGGCTCCTTGTTAGTCCAGTGCAAGCAAAAGCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

282

Amino Acids

31.79

Weight (kDa)

8.96

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 272 9.5e-59 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 248, 515
AccB7I CCANNNNNTGG 1 cut(s) 525
AccII CGCG 1 cut(s) 327
AclWI GGATC 4 cut(s) 105, 360, 536, 786
AcsI RAATTY 2 cut(s) 669, 734
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 3 cut(s) 14, 178, 714
AfiI CCNNNNNNNGG 2 cut(s) 525, 803
AgsI TTSAA 4 cut(s) 110, 161, 341, 425
AjnI CCWGG 2 cut(s) 275, 508
Alw21I GWGCWC 1 cut(s) 260
AlwI GGATC 4 cut(s) 105, 360, 536, 786
AoxI GGCC 2 cut(s) 450, 569
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 2 cut(s) 669, 734
Asp700I GAANNNNTTC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 251, 329
AspS9I GGNCC 5 cut(s) 139, 279, 451, 496, 569
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 755
AvaII GGWCC 3 cut(s) 139, 279, 496
BanI GGYRCC 2 cut(s) 248, 515
BarI GAAGNNNNNNTAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 1 cut(s) 154
BccI CCATC 3 cut(s) 372, 376, 602
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 2 cut(s) 277, 510
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 557
BfaI CTAG 3 cut(s) 272, 491, 798
BfoI RGCGCY 1 cut(s) 252
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 714
Bme1390I CCNGG 3 cut(s) 277, 510, 557
Bme18I GGWCC 3 cut(s) 139, 279, 496
BmgT120I GGNCC 5 cut(s) 139, 279, 451, 496, 569
BmiI GGNNCC 6 cut(s) 250, 453, 497, 517, 570, 815
BmrFI CCNGG 3 cut(s) 277, 510, 557
BmsI GCATC 3 cut(s) 137, 276, 565
BoxI GACNNNNGTC 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 803
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 1 cut(s) 827
BseBI CCWGG 2 cut(s) 277, 510
BseLI CCNNNNNNNGG 2 cut(s) 525, 803
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 1 cut(s) 827
BseXI GCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 452, 571
BshNI GGYRCC 2 cut(s) 248, 515
BsiHKAI GWGCWC 1 cut(s) 260
BsiSI CCGG 2 cut(s) 449, 557
BslFI GGGAC 2 cut(s) 509, 676
BslI CCNNNNNNNGG 2 cut(s) 525, 803
BsmFI GGGAC 2 cut(s) 509, 676
BsnI GGCC 2 cut(s) 452, 571
Bsp1286I GDGCHC 1 cut(s) 260
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 4 cut(s) 97, 352, 528, 778
BspANI GGCC 2 cut(s) 452, 571
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 6 cut(s) 250, 453, 497, 517, 570, 815
BspPI GGATC 4 cut(s) 105, 360, 536, 786
BspT107I GGYRCC 2 cut(s) 248, 515
BsrFI RCCGGY 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 1 cut(s) 827
BssAI RCCGGY 1 cut(s) 448
BssMI GATC 4 cut(s) 97, 352, 528, 778
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 277, 510
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 249
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 5 cut(s) 189, 247, 374, 471, 834
BstDEI CTNAG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 2 cut(s) 251, 329
BstKTI GATC 4 cut(s) 100, 355, 531, 781
BstMBI GATC 4 cut(s) 97, 352, 528, 778
BstMWI GCNNNNNNNGC 2 cut(s) 255, 470
BstNI CCWGG 2 cut(s) 277, 510
BstNSI RCATGY 2 cut(s) 187, 473
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 275, 508, 555
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 528
BstYI RGATCY 1 cut(s) 528
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 2 cut(s) 452, 571
BtsI GCAGTG 3 cut(s) 258, 296, 554
BtsIMutI CAGTG 5 cut(s) 36, 258, 296, 554, 834
Cac8I GCNNGC 5 cut(s) 189, 247, 374, 471, 834
CfoI GCGC 2 cut(s) 251, 329
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 5 cut(s) 139, 279, 451, 496, 569
CseI GACGC 3 cut(s) 80, 230, 425
Csp6I GTAC 3 cut(s) 13, 177, 713
CviAII CATG 5 cut(s) 184, 470, 484, 624, 803
CviJI RGCY 7 cut(s) 275, 452, 464, 571, 592, 814, 842
CviKI_1 RGCY 7 cut(s) 275, 452, 464, 571, 592, 814, 842
CviQI GTAC 3 cut(s) 13, 177, 713
DdeI CTNAG 1 cut(s) 330
DinI GGCGCC 1 cut(s) 250
DpnI GATC 4 cut(s) 99, 354, 530, 780
DpnII GATC 4 cut(s) 97, 352, 528, 778
DraI TTTAAA 2 cut(s) 7, 57
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco47I GGWCC 3 cut(s) 139, 279, 496
EcoO109I RGGNCCY 2 cut(s) 139, 496
EcoRII CCWGG 2 cut(s) 275, 508
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
FaeI CATG 5 cut(s) 187, 473, 487, 627, 806
FaqI GGGAC 2 cut(s) 509, 676
FatI CATG 5 cut(s) 183, 469, 483, 623, 802
FauNDI CATATG 2 cut(s) 524, 643
Fnu4HI GCNGC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 3 cut(s) 272, 491, 798
GlaI GCGC 2 cut(s) 250, 328
GluI GCNGC 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 2 cut(s) 452, 571
HapII CCGG 2 cut(s) 449, 557
HgaI GACGC 3 cut(s) 80, 230, 425
HhaI GCGC 2 cut(s) 251, 329
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 5 cut(s) 187, 473, 487, 627, 806
Hin6I GCGC 2 cut(s) 249, 327
HinP1I GCGC 2 cut(s) 249, 327
HinfI GANTC 3 cut(s) 18, 430, 664
HpaII CCGG 2 cut(s) 449, 557
HphI GGTGA 1 cut(s) 755
Hpy166II GTNNAC 2 cut(s) 70, 179
Hpy188I TCNGA 2 cut(s) 205, 756
Hpy188III TCNNGA 3 cut(s) 356, 425, 661
Hpy8I GTNNAC 2 cut(s) 70, 179
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 2 cut(s) 255, 470
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 5 cut(s) 187, 473, 487, 627, 806
HspAI GCGC 2 cut(s) 249, 327
KasI GGCGCC 1 cut(s) 248
Kzo9I GATC 4 cut(s) 97, 352, 528, 778
LmnI GCTCC 1 cut(s) 819
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 3 cut(s) 137, 276, 565
MaeI CTAG 3 cut(s) 272, 491, 798
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 3 cut(s) 294, 356, 786
MalI GATC 4 cut(s) 99, 354, 530, 780
MboI GATC 4 cut(s) 97, 352, 528, 778
MboII GAAGA 2 cut(s) 13, 523
MflI RGATCY 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 9 cut(s) 8, 86, 150, 562, 669, 734, 757, 807, 844
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 3 cut(s) 12, 439, 658
MnlI CCTC 2 cut(s) 371, 506
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 5 cut(s) 6, 56, 84, 392, 588
MspI CCGG 2 cut(s) 449, 557
MspR9I CCNGG 3 cut(s) 277, 510, 557
MvaI CCWGG 2 cut(s) 277, 510
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 255, 470
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 1 cut(s) 557
NdeI CATATG 2 cut(s) 524, 643
NdeII GATC 4 cut(s) 97, 352, 528, 778
NlaIII CATG 5 cut(s) 187, 473, 487, 627, 806
NlaIV GGNNCC 6 cut(s) 250, 453, 497, 517, 570, 815
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 2 cut(s) 294, 356
NspI RCATGY 2 cut(s) 187, 473
PaeI GCATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 525
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 3 cut(s) 12, 438, 658
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 3 cut(s) 12, 438, 658
PpuMI RGGWCCY 2 cut(s) 139, 496
PshAI GACNNNNGTC 1 cut(s) 416
Psp5II RGGWCCY 2 cut(s) 139, 496
Psp6I CCWGG 2 cut(s) 275, 508
PspGI CCWGG 2 cut(s) 275, 508
PspN4I GGNNCC 6 cut(s) 250, 453, 497, 517, 570, 815
PspPI GGNCC 5 cut(s) 139, 279, 451, 496, 569
PspPPI RGGWCCY 2 cut(s) 139, 496
PsuI RGATCY 1 cut(s) 528
RsaI GTAC 3 cut(s) 14, 178, 714
RsaNI GTAC 3 cut(s) 13, 177, 713
SaqAI TTAA 5 cut(s) 6, 56, 84, 392, 588
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 4 cut(s) 97, 352, 528, 778
Sau96I GGNCC 5 cut(s) 139, 279, 451, 496, 569
ScaI AGTACT 1 cut(s) 714
SchI GAGTC 3 cut(s) 12, 439, 658
ScrFI CCNGG 3 cut(s) 277, 510, 557
SduI GDGCHC 1 cut(s) 260
SetI ASST 7 cut(s) 69, 108, 144, 284, 322, 363, 501
SfaNI GCATC 3 cut(s) 137, 276, 565
SfoI GGCGCC 1 cut(s) 250
SinI GGWCC 3 cut(s) 139, 279, 496
SphI GCATGC 1 cut(s) 473
Sse9I AATT 9 cut(s) 8, 86, 150, 562, 669, 734, 757, 807, 844
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 3 cut(s) 272, 491, 798
StyD4I CCNGG 3 cut(s) 275, 508, 555
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 667
TasI AATT 9 cut(s) 8, 86, 150, 562, 669, 734, 757, 807, 844
TatI WGTACW 3 cut(s) 12, 176, 712
Tru1I TTAA 5 cut(s) 6, 56, 84, 392, 588
Tru9I TTAA 5 cut(s) 6, 56, 84, 392, 588
TscAI CASTG 5 cut(s) 36, 265, 303, 554, 834
TseFI GTSAC 2 cut(s) 294, 356
TseI GCWGC 1 cut(s) 167
Tsp45I GTSAC 2 cut(s) 294, 356
TspDTI ATGAA 1 cut(s) 500
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 5 cut(s) 36, 265, 303, 554, 834
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 3 cut(s) 139, 279, 496
XapI RAATTY 2 cut(s) 669, 734
XceI RCATGY 2 cut(s) 187, 473
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 3 cut(s) 272, 491, 798
ZrmI AGTACT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.