MD04G1178800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000980
Physical Location & Seq
Reverse (-)
26976772 .. 26980341
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1178800.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGCAGAGGGTCTTCAGGTAGCAATGATATGTTGGTCACTTGTGTTGATCGGTGTTTTGAGTGTGGTGATGCGATTGTTCAAGGAGATGTGGCTAAAACCTGCAAGGATCCGGTCAGTGCTTCGGAAGCAAGGCATCGGAGGGCCACCACCTTCATTCCTTGATGGGAATATGACAGAGATGCAAAAAATTCAATCCACCACCAACATCATCCATGAACCTTCTGATCCTAAAGATTTCCAGCACAATTGGGTTCCCTCCATCTTTCCGTATCTTCAGCGGTGGGAGCAGAAGTACGGTCCAGTATACATGTACTCAACGGGGAGCAAACAACACATGTATGTGAGTGATCCCAAGTTGTTAAAGGAGCTGAAACTGCAAAACTACATGGATTTGGGTGTACCGAAATATTTGAGTAAGCCACTCCTGCCCTTGATAGGCAACAGCATAATTCGGGCCAACGGACAAGATTTTGCCTACCAGAAGAAACTCATCGCTCCCGAATTCTTCCTTAACAAGGTCAAGGGCATGATGGATATGATGGAGGAATCTGCAGTGGCACTTCTTAAGACATGGGAAAGACGTTTACTAAAAAGTGAGGGAGGTGTTTTGGAGATAATAGTCGATAAAGAACTGAAGACCCTGTCTGCTGATATCATATCAAGAGCTTGTTTTGGTAGCACTTACTCCCAAGGCAACCAGATTTTTGCAAAGATTGCTCTCCTCCAGGAGGCCTTGTCAAATCCAATTTTGCTCTTTGGGGTTATCAATTTTGGCTTCTTTCCCACAGAGAATGACAAGAAGATTTGGAGTTTGAGGAAAGAGGTGGACTCTTTGTTGCTCAAATTAGTGAGGGATCGCCAAAAACAAAGCCAAGTGGGTGGCATATGTAAAAAGGACCTATTACAAATGATGCTGGAAAGTGCTGCAAGTACTAGCAGTGACATGCATCAAAACACTCACAAAACAGACCAATTTATTCTAGACAATTGTAGAAGTATCTACTTTGCAGGCTCTGAGACCACTGCTCTTACAGCTTCCTGGACATTGATGCTATTGGCATTACACCCAAAGTGGCAAGAACGTATTCGTGCCGAGATTTCTGAAGTCTGTGGAGGTGATGATCAACTGCATCATTGCTTGAAAGACACGGATAAATTGAACAAATTGAAAACGCTGACAATGGTGATTCAAGAGAGCCTGCGGTTTTATGCACCTTCAGTTTTAGTGTCAAGGGAAGCTCTAGAAAAGGTGAGGTTGGGGGACTTGGATGTGCCGAAAGGCGTCCACATATGGACATTTATTCCAACACTACACCGTGACCCCGAAAATTGGGGTCCGGATGCTGATGAGTTCAAGCCCGAGAGGTTTGCAAATGGGGTCTCTGAAGCATGCAAATATCCCCAAGCATATATGCCATTTGGTTATGGGAGCCGATTGTGTATAGGCCAAACTTTTAGCATGGTGCAGCTCAAGATAGTACTCTCTCTTACTCTATCCAAGTTCTCCTTTGCCCTTTCCCCAAACTATCAGCACTCTCCTGTTTACAAAATGTTGTTGTTGCCTAAACATGGAATCAAACTTCTTGTTAGGCGTGTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

535

Amino Acids

60.82

Weight (kDa)

9.15

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 91 - 529 3.6e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 1339
AciI CCGC 2 cut(s) 280, 1204
AclWI GGATC 5 cut(s) 103, 116, 221, 344, 864
AcsI RAATTY 2 cut(s) 189, 503
AcuI CTGAAG 5 cut(s) 260, 656, 1125, 1203, 1407
AcyI GRCGYC 1 cut(s) 1284
AfaI GTAC 5 cut(s) 296, 314, 402, 934, 1482
AfiI CCNNNNNNNGG 5 cut(s) 437, 517, 730, 1332, 1571
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 309, 336
AgsI TTSAA 7 cut(s) 82, 194, 1144, 1162, 1171, 1193, 1357
AjnI CCWGG 2 cut(s) 726, 1040
AluBI AGCT 5 cut(s) 370, 668, 1037, 1241, 1471
AluI AGCT 5 cut(s) 370, 668, 1037, 1241, 1471
Alw26I GTCTC 2 cut(s) 1013, 1387
AlwI GGATC 5 cut(s) 103, 116, 221, 344, 864
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 1 cut(s) 1361
Aor13HI TCCGGA 1 cut(s) 1339
AoxI GGCC 4 cut(s) 143, 456, 732, 1447
ApeKI GCWGC 2 cut(s) 926, 1468
ApoI RAATTY 2 cut(s) 189, 503
Asp700I GAANNNNTTC 1 cut(s) 1086
AspS9I GGNCC 5 cut(s) 143, 299, 456, 898, 1337
AsuHPI GGTGA 4 cut(s) 79, 1130, 1198, 1264
AvaI CYCGRG 1 cut(s) 1361
AvaII GGWCC 3 cut(s) 299, 898, 1337
BamHI GGATCC 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 5, 644
BbvI GCAGC 2 cut(s) 913, 1480
BccI CCATC 4 cut(s) 158, 269, 526, 535
BcgI CGANNNNNNTGC 2 cut(s) 1352, 1386
BciT130I CCWGG 2 cut(s) 728, 1042
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 2 cut(s) 1013, 1387
BfaI CTAG 3 cut(s) 936, 983, 1244
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 2 cut(s) 927, 1469
BlsI GCNGC 2 cut(s) 928, 1470
BmcAI AGTACT 2 cut(s) 934, 1482
Bme1390I CCNGG 2 cut(s) 728, 1042
Bme18I GGWCC 3 cut(s) 299, 898, 1337
BmeT110I CYCGRG 1 cut(s) 1361
BmgT120I GGNCC 5 cut(s) 143, 299, 456, 898, 1337
BmiI GGNNCC 4 cut(s) 110, 255, 1338, 1433
BmrFI CCNGG 2 cut(s) 728, 1042
BmsI GCATC 8 cut(s) 60, 144, 171, 903, 958, 1041, 1141, 1333
BpiI GAAGAC 2 cut(s) 5, 644
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 1457
BsaHI GRCGYC 1 cut(s) 1284
BsaI GGTCTC 2 cut(s) 1013, 1387
BsaJI CCNNGG 1 cut(s) 691
BsaWI WCCGGW 2 cut(s) 111, 1339
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 722, 752
Bsc4I CCNNNNNNNGG 5 cut(s) 437, 517, 730, 1332, 1571
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 2 cut(s) 30, 1135
BseAI TCCGGA 1 cut(s) 1339
BseBI CCWGG 2 cut(s) 728, 1042
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 210, 1276, 1348
BseLI CCNNNNNNNGG 5 cut(s) 437, 517, 730, 1332, 1571
BseMI GCAATG 2 cut(s) 30, 1135
BseMII CTCAG 1 cut(s) 1008
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 2 cut(s) 913, 1480
BsgI GTGCAG 1 cut(s) 1487
BshFI GGCC 4 cut(s) 145, 458, 734, 1449
BsiHKCI CYCGRG 1 cut(s) 1361
BsiSI CCGG 2 cut(s) 112, 1340
BslFI GGGAC 1 cut(s) 1277
BslI CCNNNNNNNGG 5 cut(s) 437, 517, 730, 1332, 1571
BsmAI GTCTC 2 cut(s) 1013, 1387
BsmFI GGGAC 1 cut(s) 1277
BsnI GGCC 4 cut(s) 145, 458, 734, 1449
Bso31I GGTCTC 2 cut(s) 1013, 1387
BsoBI CYCGRG 1 cut(s) 1361
Bsp13I TCCGGA 1 cut(s) 1339
Bsp143I GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
BspACI CCGC 2 cut(s) 280, 1204
BspANI GGCC 4 cut(s) 145, 458, 734, 1449
BspCNI CTCAG 1 cut(s) 1009
BspEI TCCGGA 1 cut(s) 1339
BspLI GGNNCC 4 cut(s) 110, 255, 1338, 1433
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 5 cut(s) 103, 116, 221, 344, 864
BspTI CTTAAG 1 cut(s) 566
BspTNI GGTCTC 2 cut(s) 1013, 1387
BsrDI GCAATG 2 cut(s) 30, 1135
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
BssNAI GTATAC 1 cut(s) 307
BssNI GRCGYC 1 cut(s) 1284
BssT1I CCWWGG 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 728, 1042
Bst4CI ACNGT 2 cut(s) 299, 1319
BstACI GRCGYC 1 cut(s) 1284
BstAFI CTTAAG 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 716
BstC8I GCNNGC 3 cut(s) 1012, 1202, 1393
BstDEI CTNAG 1 cut(s) 1017
BstENI CCTNNNNNAGG 2 cut(s) 515, 728
BstF5I GGATG 3 cut(s) 210, 1276, 1348
BstKTI GATC 6 cut(s) 51, 111, 229, 352, 859, 1126
BstMAI GTCTC 2 cut(s) 1013, 1387
BstMBI GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
BstMWI GCNNNNNNNGC 6 cut(s) 127, 286, 376, 427, 716, 1034
BstNI CCWGG 2 cut(s) 728, 1042
BstNSI RCATGY 4 cut(s) 313, 340, 949, 1395
BstSCI CCNGG 2 cut(s) 726, 1040
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 913, 1480
BstV2I GAAGAC 2 cut(s) 5, 644
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 881
BstYI RGATCY 1 cut(s) 108
BstZ17I GTATAC 1 cut(s) 307
BsuRI GGCC 4 cut(s) 145, 458, 734, 1449
BtgZI GCGATG 1 cut(s) 478
BtsCI GGATG 3 cut(s) 210, 1276, 1348
BtsI GCAGTG 3 cut(s) 561, 946, 1023
BtsIMutI CAGTG 4 cut(s) 123, 561, 946, 1023
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 1012, 1202, 1393
CaiI CAGNNNCTG 1 cut(s) 1016
Cfr13I GGNCC 5 cut(s) 143, 299, 456, 898, 1337
CseI GACGC 1 cut(s) 1273
Csp6I GTAC 5 cut(s) 295, 313, 401, 933, 1481
CviQI GTAC 5 cut(s) 295, 313, 401, 933, 1481
DdeI CTNAG 1 cut(s) 1017
DpnI GATC 6 cut(s) 50, 110, 228, 351, 858, 1125
DpnII GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
Eco130I CCWWGG 1 cut(s) 691
Eco147I AGGCCT 1 cut(s) 734
Eco31I GGTCTC 2 cut(s) 1013, 1387
Eco32I GATATC 1 cut(s) 655
Eco47I GGWCC 3 cut(s) 299, 898, 1337
Eco57I CTGAAG 5 cut(s) 260, 656, 1125, 1203, 1407
Eco88I CYCGRG 1 cut(s) 1361
EcoNI CCTNNNNNAGG 2 cut(s) 515, 728
EcoO109I RGGNCCY 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 2 cut(s) 726, 1040
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 691
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 1 cut(s) 1277
FauNDI CATATG 2 cut(s) 887, 1292
FbaI TGATCA 1 cut(s) 1123
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 927, 1469
FokI GGATG 3 cut(s) 197, 1283, 1355
Fsp4HI GCNGC 2 cut(s) 927, 1469
FspBI CTAG 3 cut(s) 936, 983, 1244
GluI GCNGC 2 cut(s) 927, 1469
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 4 cut(s) 145, 458, 734, 1449
HapII CCGG 2 cut(s) 112, 1340
HgaI GACGC 1 cut(s) 1273
Hin1I GRCGYC 1 cut(s) 1284
HinfI GANTC 4 cut(s) 548, 830, 1189, 1575
HpaII CCGG 2 cut(s) 112, 1340
HphI GGTGA 4 cut(s) 79, 1130, 1198, 1264
Hpy166II GTNNAC 6 cut(s) 307, 401, 587, 829, 1288, 1546
Hpy188I TCNGA 6 cut(s) 126, 140, 226, 1018, 1105, 1387
Hpy188III TCNNGA 7 cut(s) 500, 663, 983, 1193, 1244, 1340, 1474
Hpy8I GTNNAC 6 cut(s) 307, 401, 587, 829, 1288, 1546
HpyAV CCTTC 3 cut(s) 162, 231, 1227
HpyCH4III ACNGT 2 cut(s) 299, 1319
HpyCH4IV ACGT 2 cut(s) 583, 1084
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 286, 376, 427, 716, 1034
HpyF3I CTNAG 1 cut(s) 1017
HpySE526I ACGT 2 cut(s) 583, 1084
Hsp92I GRCGYC 1 cut(s) 1284
Kpn2I TCCGGA 1 cut(s) 1339
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
LmnI GCTCC 5 cut(s) 286, 324, 367, 502, 1431
Lsp1109I GCAGC 2 cut(s) 913, 1480
LweI GCATC 8 cut(s) 60, 144, 171, 903, 958, 1041, 1141, 1333
MaeI CTAG 3 cut(s) 936, 983, 1244
MaeII ACGT 2 cut(s) 583, 1084
MaeIII GTNAC 3 cut(s) 36, 941, 1319
MalI GATC 6 cut(s) 50, 110, 228, 351, 858, 1125
MboI GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
MboII GAAGA 6 cut(s) 5, 266, 496, 499, 649, 814
MfeI CAATTG 2 cut(s) 247, 988
MflI RGATCY 1 cut(s) 108
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 1 cut(s) 1331
Mph1103I ATGCAT 1 cut(s) 951
MroI TCCGGA 1 cut(s) 1339
MroXI GAANNNNTTC 1 cut(s) 1086
MseI TTAA 3 cut(s) 362, 513, 567
MslI CAYNNNNRTG 2 cut(s) 339, 341
MspA1I CMGCKG 1 cut(s) 280
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 2 cut(s) 112, 1340
MspR9I CCNGG 2 cut(s) 728, 1042
MunI CAATTG 2 cut(s) 247, 988
MvaI CCWGG 2 cut(s) 728, 1042
MwoI GCNNNNNNNGC 6 cut(s) 127, 286, 376, 427, 716, 1034
NdeI CATATG 2 cut(s) 887, 1292
NdeII GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
NlaIV GGNNCC 4 cut(s) 110, 255, 1338, 1433
NmeAIII GCCGAG 1 cut(s) 1120
NmuCI GTSAC 3 cut(s) 36, 941, 1319
NsiI ATGCAT 1 cut(s) 951
NspI RCATGY 4 cut(s) 313, 340, 949, 1395
PaeI GCATGC 1 cut(s) 1395
PceI AGGCCT 1 cut(s) 734
PciI ACATGT 2 cut(s) 309, 336
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 3 cut(s) 548, 1189, 1575
PflFI GACNNNGTC 1 cut(s) 643
PfoI TCCNGGA 2 cut(s) 726, 1040
PkrI GCNGC 2 cut(s) 928, 1470
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PpuMI RGGWCCY 1 cut(s) 898
PscI ACATGT 2 cut(s) 309, 336
Psp5II RGGWCCY 1 cut(s) 898
Psp6I CCWGG 2 cut(s) 726, 1040
PspGI CCWGG 2 cut(s) 726, 1040
PspN4I GGNNCC 4 cut(s) 110, 255, 1338, 1433
PspPI GGNCC 5 cut(s) 143, 299, 456, 898, 1337
PspPPI RGGWCCY 1 cut(s) 898
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 643
RsaI GTAC 5 cut(s) 296, 314, 402, 934, 1482
RsaNI GTAC 5 cut(s) 295, 313, 401, 933, 1481
RseI CAYNNNNRTG 2 cut(s) 339, 341
SaqAI TTAA 3 cut(s) 362, 513, 567
SatI GCNGC 2 cut(s) 927, 1469
Sau3AI GATC 6 cut(s) 48, 108, 226, 349, 856, 1123
Sau96I GGNCC 5 cut(s) 143, 299, 456, 898, 1337
ScaI AGTACT 2 cut(s) 934, 1482
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 2 cut(s) 728, 1042
SfaNI GCATC 8 cut(s) 60, 144, 171, 903, 958, 1041, 1141, 1333
SfcI CTRYAG 1 cut(s) 552
SinI GGWCC 3 cut(s) 299, 898, 1337
SmiMI CAYNNNNRTG 2 cut(s) 339, 341
SmlI CTYRAG 2 cut(s) 566, 1472
SmoI CTYRAG 2 cut(s) 566, 1472
SphI GCATGC 1 cut(s) 1395
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 2 cut(s) 280, 1204
SspI AATATT 1 cut(s) 410
SspMI CTAG 3 cut(s) 936, 983, 1244
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 2 cut(s) 726, 1040
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 2 cut(s) 299, 1319
TaiI ACGT 2 cut(s) 586, 1087
TaqI TCGA 1 cut(s) 624
TatI WGTACW 3 cut(s) 312, 932, 1480
TfiI GAWTC 3 cut(s) 548, 1189, 1575
Tru1I TTAA 3 cut(s) 362, 513, 567
Tru9I TTAA 3 cut(s) 362, 513, 567
TscAI CASTG 4 cut(s) 123, 561, 946, 1030
TseFI GTSAC 3 cut(s) 36, 941, 1319
TseI GCWGC 2 cut(s) 926, 1468
Tsp45I GTSAC 3 cut(s) 36, 941, 1319
TspDTI ATGAA 2 cut(s) 144, 231
TspGWI ACGGA 3 cut(s) 258, 477, 1166
TspRI CASTG 4 cut(s) 123, 561, 946, 1030
Tth111I GACNNNGTC 1 cut(s) 643
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 3 cut(s) 299, 898, 1337
XagI CCTNNNNNAGG 2 cut(s) 515, 728
XapI RAATTY 2 cut(s) 189, 503
XbaI TCTAGA 2 cut(s) 982, 1243
XceI RCATGY 4 cut(s) 313, 340, 949, 1395
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 3 cut(s) 936, 983, 1244
ZrmI AGTACT 2 cut(s) 934, 1482
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.