Rh7AG213700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000980
Physical Location & Seq
Reverse (-)
19725914 .. 19728160
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG213700.1

Sequence Viewer

Length: 1179 bp
ATGGCAATAGCACTGCTGCTGACAGCTTTATTTGTTTGTATATCAAGCCTAGTATTGAGAGCGCTTAACCAGTTGTGGTTTAAGCCTCGAAGAATTAGGTCAGTGCTTTATAAGCAAGGCATTCGAGGACCGAGACCAAGCTTCCCTTTCGGAAACATTCCTGAGATGCAGAAGATGCAGTTCTCCATCAAGATTCCTAGTGATGCTCAGCAACATGTGCACCACAATTGGGTTCCCTCCATATTCCCATATCTTCACAAATGGGAAAAAAAGTACGGTCCAATATACATGTACTCCACAGGGAGCAAGCAACACCTTTATGTGAGCGATCCAAATTTGTTAAGGGGGATCAAACAGCATAACACCTTGGATCTGGGTAAACCTACGTACCTGGGTAAGCCACTCCAACCCTTGCTAGGTGACGGAATTATCAGGTCTAACGGACAACACTGGGCCAACCAGAAAAAAATCATTGCTCCCGAATTCTTCCTTAACAAGGTTATGGGCATGGTGAGTTTGATGCTGGAATCTACAATGAAAATAATGAAGGAATGGGAGAGCCGTATACAAGAAGTTGGTGAGGATTCGAATGTTCTTGACATGAGTATTGATAAGGATTTGAAGACCCTCTCCCGAGATATCATCTCCAGGGCTTGTTTGAGCAGCTCTTACGCTCAAGGAAACCAGATTTTTGAAAAAATTTCTCTCATGCAGGAAGCTTTGTCGAAACCAAGTTTGCTTTTCGGCTTTCTAAATTTCAGGTTTCTTCCTTTTAACAATGACAAAAAAATATGGAATTTAAGGAGAGAAGTGGACTCTATGTTGCTCGAAATAGTGAGGCATCGCCAATCCGGTGGTGGTGTCCACAATGCACAAAAAGACCTACTACAAGTGCTACTAGAAAGTGCTGCTGCTGCCAACAATGGGAAGCATACACCTAAAACTGAACGTTTCGTTTTGGACAATTGTAGAACTATCTACTTTGTTGGCTCTGAGACCACTTCTCTGACTGCATCATGGACCTTGATGCTTCTTTCTTTGCACCCCGAGTGGCAGGAGCGTCTTCGAGCTGAGATTATTGGCATTTGTGGGGATCATGATCGACATGAACTCCATCGTTGCTTACAAGACATGGATACGTTAAGCAAATTAAAAATGGTACGGACATACTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

392

Amino Acids

45.12

Weight (kDa)

9.51

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 82 - 380 3.2e-33 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AccI GTMKAC 1 cut(s) 565
AclI AACGTT 1 cut(s) 949
AclWI GGATC 4 cut(s) 323, 356, 378, 1101
AcsI RAATTY 5 cut(s) 334, 482, 699, 754, 796
AfaI GTAC 4 cut(s) 275, 293, 389, 1161
AfeI AGCGCT 1 cut(s) 63
AfiI CCNNNNNNNGG 4 cut(s) 229, 416, 496, 924
AflIII ACRYGT 2 cut(s) 214, 288
AgsI TTSAA 3 cut(s) 622, 695, 1174
AjnI CCWGG 2 cut(s) 390, 647
AluBI AGCT 5 cut(s) 26, 141, 666, 719, 1070
AluI AGCT 5 cut(s) 26, 141, 666, 719, 1070
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 127, 989
Alw44I GTGCAC 1 cut(s) 218
AlwI GGATC 4 cut(s) 323, 356, 378, 1101
Ama87I CYCGRG 2 cut(s) 633, 1046
Aor51HI AGCGCT 1 cut(s) 63
AoxI GGCC 1 cut(s) 453
ApaLI GTGCAC 1 cut(s) 218
ApeKI GCWGC 5 cut(s) 16, 663, 908, 911, 914
ApoI RAATTY 5 cut(s) 334, 482, 699, 754, 796
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 4 cut(s) 128, 278, 453, 1020
AsuHPI GGTGA 3 cut(s) 431, 523, 590
AsuII TTCGAA 1 cut(s) 587
AvaI CYCGRG 2 cut(s) 633, 1046
AvaII GGWCC 3 cut(s) 128, 278, 1020
BaeGI GKGCMC 1 cut(s) 222
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BbsI GAAGAC 2 cut(s) 629, 1055
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 5 cut(s) 3, 675, 895, 898, 901
BccI CCATC 2 cut(s) 194, 1122
BceAI ACGGC 1 cut(s) 546
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 2 cut(s) 392, 649
BciVI GTATCC 1 cut(s) 1129
BcoDI GTCTC 2 cut(s) 127, 989
BfaI CTAG 4 cut(s) 50, 198, 416, 899
BfoI RGCGCY 1 cut(s) 65
BfuI GTATCC 1 cut(s) 1129
BisI GCNGC 5 cut(s) 17, 664, 909, 912, 915
BlpI GCTNAGC 1 cut(s) 207
BlsI GCNGC 5 cut(s) 18, 665, 910, 913, 916
Bme1390I CCNGG 2 cut(s) 392, 649
Bme18I GGWCC 3 cut(s) 128, 278, 1020
BmeT110I CYCGRG 2 cut(s) 633, 1046
BmgT120I GGNCC 4 cut(s) 128, 278, 453, 1020
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 392, 649
BmrI ACTGGG 1 cut(s) 460
BmsI GCATC 7 cut(s) 156, 165, 193, 510, 850, 1017, 1022
BmuI ACTGGG 1 cut(s) 460
BpiI GAAGAC 2 cut(s) 629, 1055
BpmI CTGGAG 1 cut(s) 631
Bpu1102I GCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 660
BsaAI YACGTR 1 cut(s) 387
BsaBI GATNNNNATC 1 cut(s) 1098
BsaI GGTCTC 2 cut(s) 127, 989
BsaJI CCNNGG 3 cut(s) 366, 391, 648
BsaWI WCCGGW 1 cut(s) 851
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 1095, 1125
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 416, 496, 924
Bse1I ACTGG 2 cut(s) 70, 455
Bse3DI GCAATG 1 cut(s) 471
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 2 cut(s) 392, 649
BseDI CCNNGG 3 cut(s) 366, 391, 648
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 4 cut(s) 229, 416, 496, 924
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 4 cut(s) 153, 221, 984, 1062
BseNI ACTGG 2 cut(s) 70, 455
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 5 cut(s) 3, 675, 895, 898, 901
BshFI GGCC 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 2 cut(s) 633, 1046
BsiSI CCGG 1 cut(s) 852
BslI CCNNNNNNNGG 4 cut(s) 229, 416, 496, 924
BsmAI GTCTC 2 cut(s) 127, 989
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 1 cut(s) 455
Bso31I GGTCTC 2 cut(s) 127, 989
BsoBI CYCGRG 2 cut(s) 633, 1046
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 5 cut(s) 328, 348, 370, 1093, 1099
Bsp1720I GCTNAGC 1 cut(s) 207
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 4 cut(s) 154, 220, 985, 1063
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 4 cut(s) 323, 356, 378, 1101
BspT104I TTCGAA 1 cut(s) 587
BspTNI GGTCTC 2 cut(s) 127, 989
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 2 cut(s) 70, 455
BssECI CCNNGG 3 cut(s) 366, 391, 648
BssMI GATC 5 cut(s) 328, 348, 370, 1093, 1099
BssNAI GTATAC 1 cut(s) 566
BssT1I CCWWGG 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 566
Bst2UI CCWGG 2 cut(s) 392, 649
Bst4CI ACNGT 1 cut(s) 278
BstAPI GCANNNNNTGC 2 cut(s) 175, 217
BstBAI YACGTR 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 4 cut(s) 162, 207, 993, 1071
BstENI CCTNNNNNAGG 1 cut(s) 494
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 5 cut(s) 331, 351, 373, 1096, 1102
BstMAI GTCTC 2 cut(s) 127, 989
BstMBI GATC 5 cut(s) 328, 348, 370, 1093, 1099
BstMWI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 914
BstNI CCWGG 2 cut(s) 392, 649
BstNSI RCATGY 2 cut(s) 218, 292
BstSCI CCNGG 2 cut(s) 390, 647
BstSLI GKGCMC 1 cut(s) 222
BstSNI TACGTA 1 cut(s) 387
BstV1I GCAGC 5 cut(s) 3, 675, 895, 898, 901
BstV2I GAAGAC 2 cut(s) 629, 1055
BstX2I RGATCY 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 854
BstYI RGATCY 1 cut(s) 370
BstZ17I GTATAC 1 cut(s) 566
BsuI GTATCC 1 cut(s) 1129
BsuRI GGCC 1 cut(s) 455
BtgZI GCGATG 1 cut(s) 827
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 3 cut(s) 11, 108, 448
Cac8I GCNNGC 1 cut(s) 308
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 4 cut(s) 128, 278, 453, 1020
CseI GACGC 1 cut(s) 1049
Csp6I GTAC 4 cut(s) 274, 292, 388, 1160
CviAII CATG 9 cut(s) 215, 289, 508, 601, 709, 1017, 1097, 1106, 1132
CviQI GTAC 4 cut(s) 274, 292, 388, 1160
DdeI CTNAG 4 cut(s) 162, 207, 993, 1071
DpnI GATC 5 cut(s) 330, 350, 372, 1095, 1101
DpnII GATC 5 cut(s) 328, 348, 370, 1093, 1099
Eco105I TACGTA 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 127, 989
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 3 cut(s) 128, 278, 1020
Eco47III AGCGCT 1 cut(s) 63
Eco88I CYCGRG 2 cut(s) 633, 1046
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 390, 647
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 9 cut(s) 218, 292, 511, 604, 712, 1020, 1100, 1109, 1135
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FatI CATG 9 cut(s) 214, 288, 507, 600, 708, 1016, 1096, 1105, 1131
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 5 cut(s) 17, 664, 909, 912, 915
Fsp4HI GCNGC 5 cut(s) 17, 664, 909, 912, 915
FspBI CTAG 4 cut(s) 50, 198, 416, 899
GlaI GCGC 1 cut(s) 63
GluI GCNGC 5 cut(s) 17, 664, 909, 912, 915
GsuI CTGGAG 1 cut(s) 631
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 455
HapII CCGG 1 cut(s) 852
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 1 cut(s) 64
Hin1II CATG 9 cut(s) 218, 292, 511, 604, 712, 1020, 1100, 1109, 1135
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HindIII AAGCTT 2 cut(s) 139, 717
HinfI GANTC 4 cut(s) 193, 527, 584, 815
HpaII CCGG 1 cut(s) 852
HphI GGTGA 3 cut(s) 431, 523, 590
Hpy166II GTNNAC 5 cut(s) 220, 380, 566, 814, 865
Hpy188I TCNGA 3 cut(s) 152, 994, 1008
Hpy188III TCNNGA 6 cut(s) 161, 190, 479, 596, 633, 1097
Hpy8I GTNNAC 5 cut(s) 220, 380, 566, 814, 865
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 3 cut(s) 386, 949, 1139
HpyCH4V TGCA 7 cut(s) 169, 178, 220, 712, 872, 1013, 1042
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 914
HpyF3I CTNAG 4 cut(s) 162, 207, 993, 1071
HpySE526I ACGT 3 cut(s) 386, 949, 1139
Hsp92II CATG 9 cut(s) 218, 292, 511, 604, 712, 1020, 1100, 1109, 1135
HspAI GCGC 1 cut(s) 62
Kzo9I GATC 5 cut(s) 328, 348, 370, 1093, 1099
LmnI GCTCC 3 cut(s) 303, 481, 1057
Lsp1109I GCAGC 5 cut(s) 3, 675, 895, 898, 901
LweI GCATC 7 cut(s) 156, 165, 193, 510, 850, 1017, 1022
MaeI CTAG 4 cut(s) 50, 198, 416, 899
MaeII ACGT 3 cut(s) 386, 949, 1139
MaeIII GTNAC 1 cut(s) 419
MalI GATC 5 cut(s) 330, 350, 372, 1095, 1101
MboI GATC 5 cut(s) 328, 348, 370, 1093, 1099
MboII GAAGA 7 cut(s) 102, 184, 245, 478, 634, 758, 1055
MfeI CAATTG 2 cut(s) 226, 964
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 6 cut(s) 96, 119, 247, 574, 638, 831
MseI TTAA 8 cut(s) 66, 81, 341, 492, 774, 800, 1142, 1151
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 852
MspR9I CCNGG 2 cut(s) 392, 649
MunI CAATTG 2 cut(s) 226, 964
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 2 cut(s) 392, 649
MwoI GCNNNNNNNGC 4 cut(s) 112, 175, 217, 914
NdeII GATC 5 cut(s) 328, 348, 370, 1093, 1099
NlaIII CATG 9 cut(s) 218, 292, 511, 604, 712, 1020, 1100, 1109, 1135
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 2 cut(s) 218, 292
NspV TTCGAA 1 cut(s) 587
PagI TCATGA 1 cut(s) 1096
PciI ACATGT 2 cut(s) 214, 288
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 193, 527, 584
PkrI GCNGC 5 cut(s) 18, 665, 910, 913, 916
PleI GAGTC 1 cut(s) 809
PpsI GAGTC 1 cut(s) 809
Ppu21I YACGTR 1 cut(s) 387
PscI ACATGT 2 cut(s) 214, 288
PsiI TTATAA 1 cut(s) 111
Psp1406I AACGTT 1 cut(s) 949
Psp6I CCWGG 2 cut(s) 390, 647
PspGI CCWGG 2 cut(s) 390, 647
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 4 cut(s) 128, 278, 453, 1020
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 4 cut(s) 275, 293, 389, 1161
RsaNI GTAC 4 cut(s) 274, 292, 388, 1160
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 8 cut(s) 66, 81, 341, 492, 774, 800, 1142, 1151
SatI GCNGC 5 cut(s) 17, 664, 909, 912, 915
Sau3AI GATC 5 cut(s) 328, 348, 370, 1093, 1099
Sau96I GGNCC 4 cut(s) 128, 278, 453, 1020
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 2 cut(s) 392, 649
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 7 cut(s) 156, 165, 193, 510, 850, 1017, 1022
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 3 cut(s) 128, 278, 1020
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 675
SmoI CTYRAG 1 cut(s) 675
SnaBI TACGTA 1 cut(s) 387
SspMI CTAG 4 cut(s) 50, 198, 416, 899
StyD4I CCNGG 2 cut(s) 390, 647
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 3 cut(s) 389, 952, 1142
TaqI TCGA 7 cut(s) 88, 124, 587, 725, 828, 1066, 1102
TaqII GACCGA 1 cut(s) 145
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 3 cut(s) 193, 527, 584
Tru1I TTAA 8 cut(s) 66, 81, 341, 492, 774, 800, 1142, 1151
Tru9I TTAA 8 cut(s) 66, 81, 341, 492, 774, 800, 1142, 1151
TscAI CASTG 3 cut(s) 18, 108, 455
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 5 cut(s) 16, 663, 908, 911, 914
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 3 cut(s) 551, 560, 1122
TspGWI ACGGA 3 cut(s) 438, 456, 1177
TspRI CASTG 3 cut(s) 18, 108, 455
VneI GTGCAC 1 cut(s) 218
VpaK11BI GGWCC 3 cut(s) 128, 278, 1020
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 5 cut(s) 334, 482, 699, 754, 796
XceI RCATGY 2 cut(s) 218, 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 4 cut(s) 50, 198, 416, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.