Rh3CG101700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000980
Physical Location & Seq
Reverse (-)
8077657 .. 8082394
4738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG101700.1

Sequence Viewer

Length: 507 bp
ATGCCTGCCCAACTTAAATCAGATAGATTTATTTTGGACACTTGTAAAAGTATGTACTTTGCAGGCGCTCAGACCACTGCTACTGCAGTAGCCTGGACCTTGATGCTTCTGTCACTGCACCATGAATGGCAAGAACGTATTCGCGCTGAGATTGTTGAAGTGTGTGGGGATCATGACCTTCATCAATGCTTGCAACACATAGATACATTACCCAAACTCAAAATGGTGGAGCATAAAAATAATCGAAAACCACTTGCTATAGCACTAGGCACTCGTGAAACCCTAATAGGTGGGGGTTCGTCTCAAGTTGGAAGCCGACTATCATCGACATCCATCCACAGGAAGAGCCGATATATATCATCCACAACACAGCCCCCCATAACCGTACCAGATCCTCCCTGGCAATGCATTCATGCTCAAGCTGTAGCAAATGATCTACTTCGTAGTATGAGTTATCTTTGCAACAAAATATTCATCCAGAGGATGGTGATCTGTATAAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

168

Amino Acids

18.96

Weight (kDa)

9.3

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 8 - 76 2.2e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 484
AccII CGCG 1 cut(s) 144
AclWI GGATC 2 cut(s) 177, 386
AfaI GTAC 2 cut(s) 56, 387
AfiI CCNNNNNNNGG 2 cut(s) 339, 484
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 2 cut(s) 92, 398
AluBI AGCT 1 cut(s) 422
AluI AGCT 1 cut(s) 422
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 2 cut(s) 177, 386
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 2 cut(s) 68, 146
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 96
BauI CACGAG 1 cut(s) 273
BccI CCATC 2 cut(s) 341, 478
BciT130I CCWGG 2 cut(s) 94, 400
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 3 cut(s) 84, 258, 423
BfoI RGCGCY 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 94, 400
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 94, 400
BmsI GCATC 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 288, 402
BsaBI GATNNNNATC 2 cut(s) 355, 488
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 484
Bse3DI GCAATG 1 cut(s) 410
Bse8I GATNNNNATC 2 cut(s) 355, 488
BseBI CCWGG 2 cut(s) 94, 400
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 5 cut(s) 329, 333, 359, 474, 489
BseJI GATNNNNATC 2 cut(s) 355, 488
BseLI CCNNNNNNNGG 2 cut(s) 339, 484
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 2 cut(s) 83, 138
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 339, 484
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 408
Bsp143I GATC 4 cut(s) 169, 391, 433, 489
BspCNI CTCAG 2 cut(s) 82, 139
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 172
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 2 cut(s) 177, 386
BspQI GCTCTTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 4 cut(s) 169, 391, 433, 489
BssSI CACGAG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 94, 400
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 6, 64, 191
BstDEI CTNAG 2 cut(s) 69, 147
BstF5I GGATG 5 cut(s) 329, 333, 359, 474, 489
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 146
BstKTI GATC 4 cut(s) 172, 394, 436, 492
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 4 cut(s) 169, 391, 433, 489
BstNI CCWGG 2 cut(s) 94, 400
BstSCI CCNGG 2 cut(s) 92, 398
BstSFI CTRYAG 3 cut(s) 84, 258, 423
BstUI CGCG 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BtsCI GGATG 5 cut(s) 329, 333, 359, 474, 489
BtsI GCAGTG 2 cut(s) 75, 113
BtsIMutI CAGTG 2 cut(s) 75, 113
Cac8I GCNNGC 3 cut(s) 6, 64, 191
CciI TCATGA 1 cut(s) 172
CfoI GCGC 2 cut(s) 68, 146
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 55, 386
CviAII CATG 3 cut(s) 122, 173, 413
CviJI RGCY 5 cut(s) 92, 315, 348, 373, 422
CviKI_1 RGCY 5 cut(s) 92, 315, 348, 373, 422
CviQI GTAC 2 cut(s) 55, 386
DdeI CTNAG 2 cut(s) 69, 147
DpnI GATC 4 cut(s) 171, 393, 435, 491
DpnII GATC 4 cut(s) 169, 391, 433, 489
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 92, 398
EcoT22I ATGCAT 1 cut(s) 410
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 3 cut(s) 125, 176, 416
FatI CATG 3 cut(s) 121, 172, 412
FokI GGATG 5 cut(s) 316, 320, 346, 461, 496
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 67, 145
HaeII RGCGCY 1 cut(s) 69
HhaI GCGC 2 cut(s) 68, 146
Hin1II CATG 3 cut(s) 125, 176, 416
Hin6I GCGC 2 cut(s) 66, 144
HinP1I GCGC 2 cut(s) 66, 144
HphI GGTGA 1 cut(s) 499
Hpy188I TCNGA 2 cut(s) 22, 72
Hpy188III TCNNGA 3 cut(s) 173, 275, 478
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 6 cut(s) 62, 86, 118, 193, 408, 462
HpyF3I CTNAG 2 cut(s) 69, 147
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 3 cut(s) 125, 176, 416
HspAI GCGC 2 cut(s) 66, 144
Kzo9I GATC 4 cut(s) 169, 391, 433, 489
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 9 cut(s) 18, 48, 79, 106, 325, 385, 402, 412, 491
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 266
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 111
MalI GATC 4 cut(s) 171, 393, 435, 491
MboI GATC 4 cut(s) 169, 391, 433, 489
MboII GAAGA 1 cut(s) 355
MflI RGATCY 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 2 cut(s) 405, 474
Mph1103I ATGCAT 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 138
MseI TTAA 1 cut(s) 15
MspR9I CCNGG 2 cut(s) 94, 400
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 2 cut(s) 94, 400
MvnI CGCG 1 cut(s) 144
NdeII GATC 4 cut(s) 169, 391, 433, 489
NlaIII CATG 3 cut(s) 125, 176, 416
NmuCI GTSAC 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 410
PagI TCATGA 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 338
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 138
PflMI CCANNNNNTGG 1 cut(s) 484
Psp6I CCWGG 2 cut(s) 92, 398
PspGI CCWGG 2 cut(s) 92, 398
PspPI GGNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 2 cut(s) 56, 387
RsaNI GTAC 2 cut(s) 55, 386
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 15
Sau3AI GATC 4 cut(s) 169, 391, 433, 489
Sau96I GGNCC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 94, 400
SetI ASST 5 cut(s) 101, 139, 180, 292, 424
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 3 cut(s) 84, 258, 423
SinI GGWCC 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 303, 417
SmoI CTYRAG 2 cut(s) 303, 417
SspI AATATT 1 cut(s) 471
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 92, 398
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 244, 326
TatI WGTACW 1 cut(s) 54
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 82, 120
TseFI GTSAC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 4 cut(s) 138, 170, 401, 463
TspRI CASTG 2 cut(s) 82, 120
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 96
XcmI CCANNNNNNNNNTGG 2 cut(s) 220, 396
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.