Rh3DG100200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000980
Physical Location & Seq
Forward (+)
7984183 .. 8001608
17426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG100200.1

Sequence Viewer

Length: 1593 bp
ATGGAAGTGAACGAATTACCGATATGGTGGTCAGTTTTGTTAATCGGTGTATCAAGCTTGGTAATCAAATTGCTTAACAAGTTGTGGTTCGAGCCTAGAAGGATTCGAACAGTGCTCTCGAAGCAAGGCATTAGAGGACCACGACCCACCTTCCCTTTCGGCAATGTCCCAGAGATGCAGAAGATTCAATCAACCGTGATCAACAACACGCATGAAGAAATGGATGGTCAACATCTCAACCACAAATGGCTTCCCTCTGTATTTCCATATATTCATAAATGGGCAAAAGAGTATGGTCCAATATACATGTACTCCACAGGGATCAACCAACACCTATATGTGAGCGATCCGCAGTTGCTAATGGAGATAAAACAGCACAACTCCCTGGATCTGGGTAAACCTACATATCTGGGTAAACCACTCCGGCCCTTGTTAGGTGATGGCCTTCTCCGAACTAATGGAAAACAGTGGGCCGTCCAGAGGAAACTTCTTGCTCCTGAATTCTTCCTTGGCAAAGTTAAGGGCATGGTGGGTGTGATGGAGGAATCTACAACAGCAATGATCAAGAGATGGGATTGTCGTATACTAGAAAGCAAATCAGGCATTGTGGACATGGAAATTGATGACGAGTTGAAGACACTATCCGCAGATATCATATCAAGAGCATGTTTTGGCAGCTCTTACTCCTTTGGAAATCAGATTTTTGAAAAGATTGCTGATATGGTGGAAGTTTATGGCAAACCAAGTCTACAATTTGGCTTTCTTAATTTCAGCTGGTTTCTTCCTAACAAGAAGGTATGGAATCTAAGGAAAGAAGTGGACTCTATGTTGCTCAAGTTGGTGACGGATCGCCAGGTTGAAAGACAATCGGGTGGTGCAACACAAAATGACCTATTACAAATGCTACTCGATATTGCTTCTACCAACACTGACATTCCTGCGCGTAGAAGAGAAACTTTCGTTCTAGACAATTGTAGAAATATCTACTTTGCAAGCTCTGCAACCACTTCTCTTACGGAATCATGGACCTTGATGCTTCTGTCATTGCACCCGGAATGGCAGGACCGCATTCGTGCGGAGATCATTGAGGTTTGTGGGGTTCTGAATAAACTCCATCATTCCTTGCACGACATGGATATGTTGCGAAAGTTTAAAGTGCTGACTATGGTGATTCAAGAGAGTATGCGTCTTTATCCATCTGGAGTCCTAATTGCAAGGGCTATTCTGGCAAATGTGAAGTTGGGCGACCTTGATGTGCCCAAGGGCACCAACATCCATGTATGCAGTGCTGCATTACATTGCGACCCTGAAAATTGGGGTCCAGATGCTGATGAGTTTAAGCCAGAGAGGTTTGCAAATGGGATATCTAATGCCTGCAAGCATCCCCAAGCCTATGTGCCATTTGGTTTTGGAACTCGAGTGTGCATAGGACAGACTTTTGCTATGACACAGCTGAAGATAGTGCTCTCTCTAATCTTGTCCAAGTTCTCATTTTCCCTTTCTCCAAACTATCGCCATTCACCTGTTCACAAAATGATACTGATGCCTAAACATGGAATCAGACTCCTTGTTGAGCCTGTGCAAACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

530

Amino Acids

60.38

Weight (kDa)

8.99

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 88 - 523 3.9e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1265
AccI GTMKAC 2 cut(s) 583, 748
AccII CGCG 1 cut(s) 943
AciI CCGC 4 cut(s) 350, 645, 1066, 1076
AclWI GGATC 4 cut(s) 329, 341, 396, 855
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 1475
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1553
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 5 cut(s) 188, 634, 707, 860, 1175
AjnI CCWGG 2 cut(s) 384, 852
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AluBI AGCT 6 cut(s) 57, 678, 774, 996, 1453, 1590
AluI AGCT 6 cut(s) 57, 678, 774, 996, 1453, 1590
Alw21I GWGCWC 2 cut(s) 117, 1467
AlwI GGATC 4 cut(s) 329, 341, 396, 855
AlwNI CAGNNNCTG 1 cut(s) 1328
Ama87I CYCGRG 1 cut(s) 1416
AoxI GGCC 3 cut(s) 425, 442, 471
ApeKI GCWGC 2 cut(s) 675, 1289
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 7 cut(s) 137, 296, 426, 471, 1026, 1063, 1319
AsuC2I CCSGG 1 cut(s) 1052
AsuHPI GGTGA 4 cut(s) 449, 853, 1180, 1512
AsuII TTCGAA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 1416
AvaII GGWCC 5 cut(s) 137, 296, 1026, 1063, 1319
BaeGI GKGCMC 2 cut(s) 1260, 1268
BanI GGYRCC 1 cut(s) 1265
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 2 cut(s) 117, 1467
BbvI GCAGC 2 cut(s) 687, 1276
BccI CCATC 6 cut(s) 218, 434, 532, 564, 1122, 1204
BceAI ACGGC 1 cut(s) 458
BciT130I CCWGG 2 cut(s) 386, 854
BclI TGATCA 2 cut(s) 198, 561
BcnI CCSGG 1 cut(s) 1052
BfaI CTAG 4 cut(s) 96, 587, 965, 1591
BisI GCNGC 2 cut(s) 676, 1290
BlsI GCNGC 2 cut(s) 677, 1291
Bme1390I CCNGG 3 cut(s) 386, 854, 1052
Bme18I GGWCC 5 cut(s) 137, 296, 1026, 1063, 1319
BmeT110I CYCGRG 1 cut(s) 1416
BmgT120I GGNCC 7 cut(s) 137, 296, 426, 471, 1026, 1063, 1319
BmiI GGNNCC 2 cut(s) 1267, 1320
BmrFI CCNGG 3 cut(s) 386, 854, 1052
BmsI GCATC 5 cut(s) 165, 1023, 1315, 1390, 1533
BpiI GAAGAC 1 cut(s) 641
BpmI CTGGAG 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 1 cut(s) 1052
BsaJI CCNNGG 3 cut(s) 384, 508, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1553
Bse3DI GCAATG 4 cut(s) 169, 564, 1043, 1297
BseBI CCWGG 2 cut(s) 386, 854
BseDI CCNNGG 3 cut(s) 384, 508, 1260
BseGI GGATG 3 cut(s) 229, 1272, 1381
BseLI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1553
BseMI GCAATG 4 cut(s) 169, 564, 1043, 1297
BseSI GKGCMC 2 cut(s) 1260, 1268
BseXI GCAGC 2 cut(s) 687, 1276
Bsh1236I CGCG 1 cut(s) 943
BshFI GGCC 3 cut(s) 427, 444, 473
BshNI GGYRCC 1 cut(s) 1265
BsiHKAI GWGCWC 2 cut(s) 117, 1467
BsiHKCI CYCGRG 1 cut(s) 1416
BsiSI CCGG 2 cut(s) 424, 1052
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 4 cut(s) 391, 434, 480, 1553
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 1068
BsnI GGCC 3 cut(s) 427, 444, 473
BsoBI CYCGRG 1 cut(s) 1416
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 4 cut(s) 117, 1260, 1268, 1467
Bsp143I GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
BspACI CCGC 4 cut(s) 350, 645, 1066, 1076
BspANI GGCC 3 cut(s) 427, 444, 473
BspFNI CGCG 1 cut(s) 943
BspLI GGNNCC 2 cut(s) 1267, 1320
BspPI GGATC 4 cut(s) 329, 341, 396, 855
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 1265
BsrDI GCAATG 4 cut(s) 169, 564, 1043, 1297
BssECI CCNNGG 3 cut(s) 384, 508, 1260
BssMI GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
BssNAI GTATAC 1 cut(s) 584
BssT1I CCWWGG 2 cut(s) 508, 1260
Bst1107I GTATAC 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 386, 854
Bst4CI ACNGT 3 cut(s) 112, 196, 468
Bst6I CTCTTC 1 cut(s) 943
BstAPI GCANNNNNTGC 1 cut(s) 998
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 994, 1375, 1379
BstDEI CTNAG 1 cut(s) 806
BstF5I GGATG 3 cut(s) 229, 1272, 1381
BstFNI CGCG 1 cut(s) 943
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 7 cut(s) 201, 324, 349, 391, 564, 850, 1083
BstMBI GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
BstMWI GCNNNNNNNGC 4 cut(s) 121, 600, 998, 1226
BstNI CCWGG 2 cut(s) 386, 854
BstNSI RCATGY 2 cut(s) 310, 669
BstSCI CCNGG 3 cut(s) 384, 852, 1050
BstSLI GKGCMC 2 cut(s) 1260, 1268
BstUI CGCG 1 cut(s) 943
BstV1I GCAGC 2 cut(s) 687, 1276
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BstZ17I GTATAC 1 cut(s) 584
BsuRI GGCC 3 cut(s) 427, 444, 473
BtsCI GGATG 3 cut(s) 229, 1272, 1381
BtsI GCAGTG 1 cut(s) 1291
BtsIMutI CAGTG 4 cut(s) 117, 473, 927, 1291
Cac8I GCNNGC 3 cut(s) 994, 1375, 1379
CaiI CAGNNNCTG 1 cut(s) 1328
CfoI GCGC 1 cut(s) 943
Cfr13I GGNCC 7 cut(s) 137, 296, 426, 471, 1026, 1063, 1319
CseI GACGC 1 cut(s) 1175
Csp6I GTAC 1 cut(s) 310
CviAII CATG 9 cut(s) 212, 307, 526, 613, 666, 1023, 1132, 1277, 1553
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 1 cut(s) 806
DpnI GATC 7 cut(s) 200, 323, 348, 390, 563, 849, 1082
DpnII GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
DraI TTTAAA 1 cut(s) 1153
Eam1104I CTCTTC 1 cut(s) 943
EarI CTCTTC 1 cut(s) 943
Eco130I CCWWGG 2 cut(s) 508, 1260
Eco32I GATATC 2 cut(s) 652, 1365
Eco47I GGWCC 5 cut(s) 137, 296, 1026, 1063, 1319
Eco57I CTGAAG 1 cut(s) 1475
Eco88I CYCGRG 1 cut(s) 1416
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 2 cut(s) 384, 852
EcoRV GATATC 2 cut(s) 652, 1365
EcoT14I CCWWGG 2 cut(s) 508, 1260
ErhI CCWWGG 2 cut(s) 508, 1260
FaeI CATG 9 cut(s) 215, 310, 529, 616, 669, 1026, 1135, 1280, 1556
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 1 cut(s) 152
FatI CATG 9 cut(s) 211, 306, 525, 612, 665, 1022, 1131, 1276, 1552
FbaI TGATCA 2 cut(s) 198, 561
FblI GTMKAC 2 cut(s) 583, 748
Fnu4HI GCNGC 2 cut(s) 676, 1290
FokI GGATG 3 cut(s) 236, 1259, 1368
Fsp4HI GCNGC 2 cut(s) 676, 1290
FspBI CTAG 4 cut(s) 96, 587, 965, 1591
GlaI GCGC 1 cut(s) 942
GluI GCNGC 2 cut(s) 676, 1290
GsuI CTGGAG 1 cut(s) 1221
HaeIII GGCC 3 cut(s) 427, 444, 473
HapII CCGG 2 cut(s) 424, 1052
HgaI GACGC 1 cut(s) 1175
HhaI GCGC 1 cut(s) 943
Hin1II CATG 9 cut(s) 215, 310, 529, 616, 669, 1026, 1135, 1280, 1556
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HincII GTYRAC 1 cut(s) 230
HindII GTYRAC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 55
HpaII CCGG 2 cut(s) 424, 1052
HphI GGTGA 4 cut(s) 449, 853, 1180, 1512
Hpy166II GTNNAC 9 cut(s) 10, 230, 398, 416, 584, 610, 749, 820, 1528
Hpy188I TCNGA 4 cut(s) 452, 699, 1104, 1562
Hpy188III TCNNGA 9 cut(s) 118, 478, 497, 565, 660, 965, 1175, 1200, 1322
Hpy8I GTNNAC 9 cut(s) 10, 230, 398, 416, 584, 610, 749, 820, 1528
HpyAV CCTTC 4 cut(s) 93, 160, 455, 787
HpyCH4III ACNGT 3 cut(s) 112, 196, 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 600, 998, 1226
HpyF3I CTNAG 1 cut(s) 806
Hsp92II CATG 9 cut(s) 215, 310, 529, 616, 669, 1026, 1135, 1280, 1556
HspAI GCGC 1 cut(s) 941
Ksp22I TGATCA 2 cut(s) 198, 561
Kzo9I GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
LmnI GCTCC 1 cut(s) 499
Lsp1109I GCAGC 2 cut(s) 687, 1276
LweI GCATC 5 cut(s) 165, 1023, 1315, 1390, 1533
MaeI CTAG 4 cut(s) 96, 587, 965, 1591
MaeIII GTNAC 1 cut(s) 841
MalI GATC 7 cut(s) 200, 323, 348, 390, 563, 849, 1082
MboI GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
MboII GAAGA 7 cut(s) 193, 227, 496, 646, 773, 960, 1468
MfeI CAATTG 1 cut(s) 970
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 4 cut(s) 117, 1260, 1268, 1467
MluCI AATT 9 cut(s) 14, 68, 500, 618, 752, 766, 970, 1209, 1312
MlyI GAGTC 3 cut(s) 815, 1212, 1557
MnlI CCTC 6 cut(s) 128, 265, 474, 535, 1081, 1341
MseI TTAA 6 cut(s) 41, 75, 519, 765, 1152, 1338
MslI CAYNNNNRTG 2 cut(s) 336, 1136
MspA1I CMGCKG 2 cut(s) 774, 1453
MspI CCGG 2 cut(s) 424, 1052
MspR9I CCNGG 3 cut(s) 386, 854, 1052
MunI CAATTG 1 cut(s) 970
Mva1269I GAATGC 1 cut(s) 1068
MvaI CCWGG 2 cut(s) 386, 854
MvnI CGCG 1 cut(s) 943
MwoI GCNNNNNNNGC 4 cut(s) 121, 600, 998, 1226
NciI CCSGG 1 cut(s) 1052
NdeII GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
NlaIII CATG 9 cut(s) 215, 310, 529, 616, 669, 1026, 1135, 1280, 1556
NlaIV GGNNCC 2 cut(s) 1267, 1320
NmuCI GTSAC 1 cut(s) 841
NspI RCATGY 2 cut(s) 310, 669
NspV TTCGAA 1 cut(s) 106
PaeR7I CTCGAG 1 cut(s) 1416
PciI ACATGT 1 cut(s) 306
PctI GAATGC 1 cut(s) 1068
PfeI GAWTC 7 cut(s) 103, 184, 545, 802, 1019, 1171, 1557
PkrI GCNGC 2 cut(s) 677, 1291
PleI GAGTC 3 cut(s) 815, 1211, 1557
PpsI GAGTC 3 cut(s) 815, 1211, 1557
PscI ACATGT 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 384, 852
PspGI CCWGG 2 cut(s) 384, 852
PspN4I GGNNCC 2 cut(s) 1267, 1320
PspPI GGNCC 7 cut(s) 137, 296, 426, 471, 1026, 1063, 1319
PspXI VCTCGAGB 1 cut(s) 1416
PstNI CAGNNNCTG 1 cut(s) 1328
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 2 cut(s) 774, 1453
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 2 cut(s) 336, 1136
SaqAI TTAA 6 cut(s) 41, 75, 519, 765, 1152, 1338
SatI GCNGC 2 cut(s) 676, 1290
Sau3AI GATC 7 cut(s) 198, 321, 346, 388, 561, 847, 1080
Sau96I GGNCC 7 cut(s) 137, 296, 426, 471, 1026, 1063, 1319
SchI GAGTC 3 cut(s) 815, 1212, 1557
ScrFI CCNGG 3 cut(s) 386, 854, 1052
SduI GDGCHC 4 cut(s) 117, 1260, 1268, 1467
SfaNI GCATC 5 cut(s) 165, 1023, 1315, 1390, 1533
Sfr274I CTCGAG 1 cut(s) 1416
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 5 cut(s) 137, 296, 1026, 1063, 1319
SlaI CTCGAG 1 cut(s) 1416
SmiMI CAYNNNNRTG 2 cut(s) 336, 1136
SmlI CTYRAG 2 cut(s) 833, 1416
SmoI CTYRAG 2 cut(s) 833, 1416
Sse9I AATT 9 cut(s) 14, 68, 500, 618, 752, 766, 970, 1209, 1312
SsiI CCGC 4 cut(s) 350, 645, 1066, 1076
SspMI CTAG 4 cut(s) 96, 587, 965, 1591
StyD4I CCNGG 3 cut(s) 384, 852, 1050
StyI CCWWGG 2 cut(s) 508, 1260
TaaI ACNGT 3 cut(s) 112, 196, 468
TaqI TCGA 5 cut(s) 90, 106, 119, 909, 1417
TasI AATT 9 cut(s) 14, 68, 500, 618, 752, 766, 970, 1209, 1312
TatI WGTACW 1 cut(s) 309
TfiI GAWTC 7 cut(s) 103, 184, 545, 802, 1019, 1171, 1557
Tru1I TTAA 6 cut(s) 41, 75, 519, 765, 1152, 1338
Tru9I TTAA 6 cut(s) 41, 75, 519, 765, 1152, 1338
TscAI CASTG 4 cut(s) 117, 473, 934, 1291
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 2 cut(s) 675, 1289
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 2 cut(s) 228, 263
TspGWI ACGGA 2 cut(s) 860, 1031
TspRI CASTG 4 cut(s) 117, 473, 934, 1291
VpaK11BI GGWCC 5 cut(s) 137, 296, 1026, 1063, 1319
XapI RAATTY 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 964
XceI RCATGY 2 cut(s) 310, 669
XhoI CTCGAG 1 cut(s) 1416
XmiI GTMKAC 2 cut(s) 583, 748
XspI CTAG 4 cut(s) 96, 587, 965, 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.