Rroxscaffold_6G00419950

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000980
Physical Location & Seq
Forward (+)
41301217 .. 41303649
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00419950.1

Sequence Viewer

Length: 1389 bp
ATGGCAGCCCTGTTGATATTTTGGACAATTTTCTTCCTTGGCATTCTGAGCCTGTGTACGAGATTGCTTAACCAGTTATGGCTCAAACCTAGAAGGATTCGATCAGTGCTGTGGAAGCAAGGCATCAGAGGTCCGCAGCCTTCATTTCTTTTCGGAAACACTGCCGAGATGGAGAAGATTCGGTCCACCGCCGATATCACCCAGAAAGCATCGAAAGGTGGGCATGTACAGCACGATTGGGCACCCTCCGTACACCCATTTCTTCATAAATGGGCAAACGAGTATGGTCCAGTATACATGTACTCCACAGGCAGCCAGCAACACCTTGTTGTAAGCGATCCACAGTTAATAAGAGAGCTTAGATCGCACAACTCTATGGATTTGGGTAGACCAACATATATGAATAAGAACTTGCAAGCTTTAACAGGTGATAGCTTTTTGGTCGCTAACGGAGAAAAGTGGGCTTACCAGAGGAAGATCATTGCCCCTGAATTTTTCCTTGACAAGGTTAAGGATATGTTCAAATTCTACAGATTCTTTCCCACAGAGAATGACAAGAAGATAAAGAGTTTAAAGAAAGAGGTGGACGCTTTGATACTAAAATTAGTGAGGGATCGTCAGGTTGAAATGCGACGGGATGGTGCATCTCAAAAGGACCTATTACAAATTATACTTGAAAGTGCTGCAAATAGTAGTGACATGCCTGCCCAACTTAAATCAGATAGATTTATTTTGGACACTTGTAAAAGTATGTACTTTGCAGGCGCTCAGTCCACTGCTACTGCAGTAGCCTGGACCTTGATGCTTCTGTCACTGCACCAAGAATGGCAAGAACGTATTCGTGCTGAGATTGTTGAAGTGTGTGGGGATCATGACCTCCATCAATGCTTGCAACACATAGATACATTACCCAAACTCAAAATGCTAACAATGGTGATTCAAGAGAGTCTGCGTCTTTATCCACCAGCAACAATAACAGCCAAGCATGCTTTAGCAGACATAAAACTAGGGGAGCTTGATGTGCCAGGAGGCACCAACATATGGATCTTCGCACACAGATTGCACCGCGACCCTGAAAATTGGGGGCCAGATGCTAATGAGTTTAAGCCAGAGAGATTTGCAAATGGGGTTTCCAAATCGTGCAAGTATACCCAAGCATATGTGCCATTTGGTTTCGGGGCTCGAATTTGCATAGGCCAGAACTTTGCTATGACGCAAATGAAGATAATACTCTCCCTAATCCTTTCCAAATTCTCATTTTCTATTTCTCCGAATTATCACCATTCTCCTGTCTCTCCAATGTTACTATTGCCTGAACATGGAATTAGGCTCCTTGTTAGTCCTGTGCAAGCAAAAGCCAATACGCTGGTTTTAAAATTAAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

462

Amino Acids

52.76

Weight (kDa)

9.49

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 83 - 173 1e-08 Cytochrome P450
p450 PF00067 172 - 445 3.4e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 241, 1031
AccB7I CCANNNNNTGG 1 cut(s) 1041
AccI GTMKAC 3 cut(s) 294, 388, 1148
AccII CGCG 1 cut(s) 1068
AciI CCGC 3 cut(s) 134, 189, 1066
AclWI GGATC 4 cut(s) 332, 621, 876, 1052
AcsI RAATTY 4 cut(s) 491, 524, 1185, 1250
AfaI GTAC 5 cut(s) 58, 228, 252, 302, 755
AfiI CCNNNNNNNGG 3 cut(s) 505, 1041, 1319
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 5 cut(s) 523, 626, 677, 857, 941
AjnI CCWGG 2 cut(s) 791, 1024
AluBI AGCT 4 cut(s) 358, 419, 435, 1015
AluI AGCT 4 cut(s) 358, 419, 435, 1015
Alw26I GTCTC 1 cut(s) 1297
AlwI GGATC 4 cut(s) 332, 621, 876, 1052
AoxI GGCC 2 cut(s) 1085, 1195
ApeKI GCWGC 4 cut(s) 5, 136, 312, 683
ApoI RAATTY 4 cut(s) 491, 524, 1185, 1250
Asp700I GAANNNNTTC 1 cut(s) 837
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 6 cut(s) 131, 183, 287, 655, 795, 1085
AsuHPI GGTGA 4 cut(s) 190, 440, 946, 1271
AvaII GGWCC 5 cut(s) 131, 183, 287, 655, 795
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 241, 1031
BanII GRGCYC 1 cut(s) 1183
BbvI GCAGC 4 cut(s) 17, 148, 324, 670
BccI CCATC 3 cut(s) 163, 632, 888
BciT130I CCWGG 2 cut(s) 793, 1026
BcoDI GTCTC 1 cut(s) 1297
BfaI CTAG 2 cut(s) 90, 1007
BfmI CTRYAG 2 cut(s) 529, 783
BfoI RGCGCY 1 cut(s) 768
BisI GCNGC 4 cut(s) 6, 137, 313, 684
BlsI GCNGC 4 cut(s) 7, 138, 314, 685
Bme1390I CCNGG 2 cut(s) 793, 1026
Bme18I GGWCC 5 cut(s) 131, 183, 287, 655, 795
BmgT120I GGNCC 6 cut(s) 131, 183, 287, 655, 795, 1085
BmiI GGNNCC 4 cut(s) 243, 1033, 1086, 1331
BmrFI CCNGG 2 cut(s) 793, 1026
BmsI GCATC 5 cut(s) 132, 218, 653, 792, 1081
BsaJI CCNNGG 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 1041, 1319
Bse1I ACTGG 2 cut(s) 73, 290
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 2 cut(s) 793, 1026
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 643
BseLI CCNNNNNNNGG 3 cut(s) 505, 1041, 1319
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 3 cut(s) 38, 782, 837
BseNI ACTGG 2 cut(s) 73, 290
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 4 cut(s) 17, 148, 324, 670
BsgI GTGCAG 1 cut(s) 800
Bsh1236I CGCG 1 cut(s) 1068
BshFI GGCC 2 cut(s) 1087, 1197
BshNI GGYRCC 2 cut(s) 241, 1031
BslI CCNNNNNNNGG 3 cut(s) 505, 1041, 1319
BsmAI GTCTC 1 cut(s) 1297
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 2 cut(s) 1087, 1197
Bsp1286I GDGCHC 2 cut(s) 244, 1183
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
BspACI CCGC 3 cut(s) 134, 189, 1066
BspANI GGCC 2 cut(s) 1087, 1197
BspCNI CTCAG 3 cut(s) 39, 781, 838
BspFNI CGCG 1 cut(s) 1068
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 4 cut(s) 243, 1033, 1086, 1331
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 4 cut(s) 332, 621, 876, 1052
BspT107I GGYRCC 2 cut(s) 241, 1031
BsrDI GCAATG 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 2 cut(s) 73, 290
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
BssNAI GTATAC 2 cut(s) 295, 1149
BssT1I CCWWGG 1 cut(s) 37
Bst1107I GTATAC 2 cut(s) 295, 1149
Bst2UI CCWGG 2 cut(s) 793, 1026
Bst4CI ACNGT 1 cut(s) 345
BstAUI TGTACA 1 cut(s) 226
BstC8I GCNNGC 7 cut(s) 317, 417, 705, 763, 890, 987, 1350
BstDEI CTNAG 4 cut(s) 47, 359, 768, 846
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 1 cut(s) 1068
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 7 cut(s) 104, 340, 365, 480, 616, 871, 1047
BstMAI GTCTC 1 cut(s) 1297
BstMBI GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
BstMWI GCNNNNNNNGC 6 cut(s) 48, 115, 229, 364, 986, 1021
BstNI CCWGG 2 cut(s) 793, 1026
BstNSI RCATGY 4 cut(s) 227, 301, 703, 989
BstSCI CCNGG 2 cut(s) 791, 1024
BstSFI CTRYAG 2 cut(s) 529, 783
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 1068
BstV1I GCAGC 4 cut(s) 17, 148, 324, 670
BstX2I RGATCY 1 cut(s) 1044
BstXI CCANNNNNNTGG 1 cut(s) 1366
BstYI RGATCY 1 cut(s) 1044
BstZ17I GTATAC 2 cut(s) 295, 1149
BsuRI GGCC 2 cut(s) 1087, 1197
BtsCI GGATG 1 cut(s) 643
BtsI GCAGTG 3 cut(s) 159, 774, 812
BtsIMutI CAGTG 4 cut(s) 111, 159, 774, 812
Cac8I GCNNGC 7 cut(s) 317, 417, 705, 763, 890, 987, 1350
CciI TCATGA 1 cut(s) 871
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 6 cut(s) 131, 183, 287, 655, 795, 1085
CseI GACGC 3 cut(s) 596, 941, 1222
Csp6I GTAC 5 cut(s) 57, 227, 251, 301, 754
CviAII CATG 6 cut(s) 224, 298, 700, 872, 986, 1319
CviQI GTAC 5 cut(s) 57, 227, 251, 301, 754
DdeI CTNAG 4 cut(s) 47, 359, 768, 846
DpnI GATC 7 cut(s) 103, 339, 364, 479, 615, 870, 1046
DpnII GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
DraI TTTAAA 2 cut(s) 573, 1374
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 1183
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 5 cut(s) 131, 183, 287, 655, 795
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 791, 1024
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 1183
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 6 cut(s) 227, 301, 703, 875, 989, 1322
FatI CATG 6 cut(s) 223, 297, 699, 871, 985, 1318
FauNDI CATATG 2 cut(s) 1040, 1159
FblI GTMKAC 3 cut(s) 294, 388, 1148
Fnu4HI GCNGC 4 cut(s) 6, 137, 313, 684
FokI GGATG 1 cut(s) 650
FriOI GRGCYC 1 cut(s) 1183
Fsp4HI GCNGC 4 cut(s) 6, 137, 313, 684
FspBI CTAG 2 cut(s) 90, 1007
GlaI GCGC 1 cut(s) 766
GluI GCNGC 4 cut(s) 6, 137, 313, 684
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 2 cut(s) 1087, 1197
HgaI GACGC 3 cut(s) 596, 941, 1222
HhaI GCGC 1 cut(s) 767
Hin1II CATG 6 cut(s) 227, 301, 703, 875, 989, 1322
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 5 cut(s) 97, 178, 534, 937, 946
HphI GGTGA 4 cut(s) 190, 440, 946, 1271
Hpy166II GTNNAC 8 cut(s) 57, 186, 253, 295, 389, 586, 774, 1149
Hpy188I TCNGA 5 cut(s) 48, 128, 155, 721, 1272
Hpy188III TCNNGA 2 cut(s) 872, 941
Hpy8I GTNNAC 8 cut(s) 57, 186, 253, 295, 389, 586, 774, 1149
Hpy99I CGWCG 1 cut(s) 636
HpyAV CCTTC 2 cut(s) 87, 150
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 1 cut(s) 835
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 115, 229, 364, 986, 1021
HpyF3I CTNAG 4 cut(s) 47, 359, 768, 846
HpySE526I ACGT 1 cut(s) 835
Hsp92II CATG 6 cut(s) 227, 301, 703, 875, 989, 1322
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
LmnI GCTCC 2 cut(s) 1012, 1335
Lsp1109I GCAGC 4 cut(s) 17, 148, 324, 670
LweI GCATC 5 cut(s) 132, 218, 653, 792, 1081
MaeI CTAG 2 cut(s) 90, 1007
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 3 cut(s) 695, 810, 1302
MalI GATC 7 cut(s) 103, 339, 364, 479, 615, 870, 1046
MboI GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
MboII GAAGA 7 cut(s) 25, 187, 254, 487, 571, 1039, 1234
MflI RGATCY 1 cut(s) 1044
MhlI GDGCHC 2 cut(s) 244, 1183
MlyI GAGTC 1 cut(s) 955
MnlI CCTC 7 cut(s) 122, 256, 465, 574, 603, 887, 1022
MroXI GAANNNNTTC 1 cut(s) 837
MseI TTAA 9 cut(s) 69, 347, 422, 510, 572, 714, 1104, 1373, 1379
MspR9I CCNGG 2 cut(s) 793, 1026
Mva1269I GAATGC 1 cut(s) 42
MvaI CCWGG 2 cut(s) 793, 1026
MvnI CGCG 1 cut(s) 1068
MwoI GCNNNNNNNGC 6 cut(s) 48, 115, 229, 364, 986, 1021
NdeI CATATG 2 cut(s) 1040, 1159
NdeII GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
NlaIII CATG 6 cut(s) 227, 301, 703, 875, 989, 1322
NlaIV GGNNCC 4 cut(s) 243, 1033, 1086, 1331
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 695, 810
NspI RCATGY 4 cut(s) 227, 301, 703, 989
PaeI GCATGC 1 cut(s) 989
PagI TCATGA 1 cut(s) 871
PciI ACATGT 1 cut(s) 297
PctI GAATGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 4 cut(s) 97, 178, 534, 937
PflMI CCANNNNNTGG 1 cut(s) 1041
PkrI GCNGC 4 cut(s) 7, 138, 314, 685
PleI GAGTC 1 cut(s) 954
PpsI GAGTC 1 cut(s) 954
PpuMI RGGWCCY 1 cut(s) 655
PscI ACATGT 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 655
Psp6I CCWGG 2 cut(s) 791, 1024
PspGI CCWGG 2 cut(s) 791, 1024
PspN4I GGNNCC 4 cut(s) 243, 1033, 1086, 1331
PspPI GGNCC 6 cut(s) 131, 183, 287, 655, 795, 1085
PspPPI RGGWCCY 1 cut(s) 655
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 1044
RsaI GTAC 5 cut(s) 58, 228, 252, 302, 755
RsaNI GTAC 5 cut(s) 57, 227, 251, 301, 754
SaqAI TTAA 9 cut(s) 69, 347, 422, 510, 572, 714, 1104, 1373, 1379
SatI GCNGC 4 cut(s) 6, 137, 313, 684
Sau3AI GATC 7 cut(s) 101, 337, 362, 477, 613, 868, 1044
Sau96I GGNCC 6 cut(s) 131, 183, 287, 655, 795, 1085
SchI GAGTC 1 cut(s) 955
ScrFI CCNGG 2 cut(s) 793, 1026
SduI GDGCHC 2 cut(s) 244, 1183
SfaNI GCATC 5 cut(s) 132, 218, 653, 792, 1081
SfcI CTRYAG 2 cut(s) 529, 783
SinI GGWCC 5 cut(s) 131, 183, 287, 655, 795
SphI GCATGC 1 cut(s) 989
SsiI CCGC 3 cut(s) 134, 189, 1066
SspMI CTAG 2 cut(s) 90, 1007
StyD4I CCNGG 2 cut(s) 791, 1024
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 1 cut(s) 838
TaqI TCGA 3 cut(s) 100, 212, 1183
TaqII GACCGA 1 cut(s) 171
TatI WGTACW 3 cut(s) 226, 300, 753
TfiI GAWTC 4 cut(s) 97, 178, 534, 937
Tru1I TTAA 9 cut(s) 69, 347, 422, 510, 572, 714, 1104, 1373, 1379
Tru9I TTAA 9 cut(s) 69, 347, 422, 510, 572, 714, 1104, 1373, 1379
TscAI CASTG 4 cut(s) 111, 166, 781, 819
TseFI GTSAC 2 cut(s) 695, 810
TseI GCWGC 4 cut(s) 5, 136, 312, 683
Tsp45I GTSAC 2 cut(s) 695, 810
TspDTI ATGAA 4 cut(s) 132, 254, 416, 1235
TspGWI ACGGA 2 cut(s) 238, 465
TspRI CASTG 4 cut(s) 111, 166, 781, 819
Van91I CCANNNNNTGG 1 cut(s) 1041
VpaK11BI GGWCC 5 cut(s) 131, 183, 287, 655, 795
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 4 cut(s) 491, 524, 1185, 1250
XceI RCATGY 4 cut(s) 227, 301, 703, 989
XmiI GTMKAC 3 cut(s) 294, 388, 1148
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 2 cut(s) 90, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.