RLG00000025055

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000980
Physical Location & Seq
Forward (+)
41623269 .. 41625677
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025055

Sequence Viewer

Length: 1584 bp
ATGGCAGCCCTGTTGATATTTTGGACAATTTTCTTCCTTGGCATTCTGAGCCTGTTTACGAGATTGCTTAACCAGTTATGGCTCAAACCTAGAAGGATTCGATCAGTGCCGTGGAAGCAAGGCATCAGAGGTCCGCAGCCTTCATTTCTTTTCCGAAACATTGCCGAGATGGAGAAGATTCGGTCCACCGCCGATATCACCCAGAAAGCATCGAAAGGTGGGCATGTACAGCACGATTGGGCACCCTCCGTACACCATTTCTTCATAAATGGGCAGACGAGTCCATTATACATGTACTCCACAGGCAGCCAGCAACACCTTGTTGTAAGCGATCCACAGTTAATAAGAGAGCTTAGATCGCACAACTCCATGGATTTGGGTAGACCAACATATATGAATAAGAACTTGCAAGCTTTAGCAGGTGATAGCTTTTTGGTCGCTAACGGAGAAAAGTGGGCTTACCAGAGGAAGATCATTGCCCCTGAATTTTTCCTTGACAAGGTTAAGGACATGTTAGGTCTGATGGTGAAATCCACAACGACAACGATTAAGGCATGGGAGAAATCTATAAAAGAAAGCGATTCGGGCATTGTGGATATGAGAATTGATGAGGATTTGAAGGCCATATCTAGAGAAGTCCTATCAAGAGCTTGCTTTGGTGGGTCTTACTCTAAAGGCAACCAGATTTTTGCAACCATTTTTGTTCTGGAAGAAGCCTTGTCCGAACCAAATGTGTTCTTTGGGTTTCTAAACTTGAGATTCTTTCCCACAGAGAATGACAAGAAGATAAAGAGTTTAAAGAAAGAGGTGGACGCTTTGATACTTAAATTAGTGAGGGATCGTCAGGTTGAAATGCCACGGGATGGTGCATCTCAAAAGGACCTGTTACAAATTATACTTGAAAGTGCTGCAAATAGTAGTGACATGCCTGACCAACTTAAATCAGATAGATTTATTTTGGACACTTGTAAAATTATGTACTTTGCAGGCGCTCAGACCACTGCTACTGCAGTAGCCTGGACCTTGATGCTTCTGTCACTGCACCAAGAATGGCAAGAACGTATTTGCGCTGAGATTGTTGAAGTGTGTGGGGATCATGACCTCCATCAATGCTTGCAACACATAGATACATTACCCAAACTCAAAATGCTAACAATGGTGATTCAAGAGAGTCTGCGTCTTTATCCACCAGCAACAATAACAGCCAAGCCTGCTTTAGCAGACATAAAACTAGGGGAGCTTGATGTGCCAGGAGGCACCAACATATGGATCTTCGCACACAGATTGCACCGCGACCCTGAAAATTGGGGGCCAGATGCTAATGAGTTTAAGCCAGAGAGATTTGTAAATGGGGTTTCCAAATCGTGCAAGTATACCCAAGCATATGTGCCATTTGGTTTCGGGGCTCGAATTTGCATAGGCCAGAACTTTGCTATGACGCAAATGAAGATAATACTCTCCCTAATCCTTTCCAAGTTCTCATTTTCTATTTCTCCGAATTATCACCATTCTCCTGTCTTTCCAATGTTACTATTGCCTGAACATGGAATTAGGCTCCTTGTTAGTCCTGTTGCAAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

528

Amino Acids

59.84

Weight (kDa)

9.03

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 92 - 520 3.3e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 241, 1256
AccB7I CCANNNNNTGG 2 cut(s) 863, 1266
AccI GTMKAC 2 cut(s) 382, 1373
AccII CGCG 1 cut(s) 1293
AciI CCGC 3 cut(s) 134, 189, 1291
AclWI GGATC 4 cut(s) 326, 846, 1101, 1277
AcsI RAATTY 2 cut(s) 485, 1410
AfaI GTAC 4 cut(s) 228, 252, 296, 980
AfiI CCNNNNNNNGG 4 cut(s) 499, 863, 1266, 1544
AflIII ACRYGT 2 cut(s) 291, 510
AgsI TTSAA 5 cut(s) 619, 851, 902, 1082, 1166
AjnI CCWGG 2 cut(s) 1016, 1249
AluBI AGCT 5 cut(s) 352, 413, 429, 650, 1240
AluI AGCT 5 cut(s) 352, 413, 429, 650, 1240
AlwI GGATC 4 cut(s) 326, 846, 1101, 1277
AoxI GGCC 3 cut(s) 621, 1310, 1420
ApeKI GCWGC 4 cut(s) 5, 136, 306, 908
ApoI RAATTY 2 cut(s) 485, 1410
AspLEI GCGC 2 cut(s) 992, 1070
AspS9I GGNCC 5 cut(s) 131, 183, 880, 1020, 1310
AsuHPI GGTGA 5 cut(s) 190, 434, 538, 1171, 1496
AvaII GGWCC 4 cut(s) 131, 183, 880, 1020
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 241, 1256
BanII GRGCYC 1 cut(s) 1408
BbvI GCAGC 4 cut(s) 17, 148, 318, 895
BccI CCATC 4 cut(s) 163, 517, 857, 1113
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 2 cut(s) 1018, 1251
BfaI CTAG 3 cut(s) 90, 630, 1232
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 993
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 4 cut(s) 6, 137, 307, 909
BlsI GCNGC 4 cut(s) 7, 138, 308, 910
Bme1390I CCNGG 2 cut(s) 1018, 1251
Bme18I GGWCC 4 cut(s) 131, 183, 880, 1020
BmgT120I GGNCC 5 cut(s) 131, 183, 880, 1020, 1310
BmiI GGNNCC 4 cut(s) 243, 1258, 1311, 1556
BmrFI CCNGG 2 cut(s) 1018, 1251
BmsI GCATC 5 cut(s) 132, 218, 878, 1017, 1306
BpuEI CTTGAG 1 cut(s) 775
BsaJI CCNNGG 4 cut(s) 37, 110, 369, 857
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 4 cut(s) 499, 863, 1266, 1544
Bse1I ACTGG 1 cut(s) 73
Bse3DI GCAATG 2 cut(s) 159, 474
BseBI CCWGG 2 cut(s) 1018, 1251
BseDI CCNNGG 4 cut(s) 37, 110, 369, 857
BseGI GGATG 1 cut(s) 868
BseLI CCNNNNNNNGG 4 cut(s) 499, 863, 1266, 1544
BseMI GCAATG 2 cut(s) 159, 474
BseMII CTCAG 3 cut(s) 38, 1007, 1062
BseNI ACTGG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 4 cut(s) 17, 148, 318, 895
BsgI GTGCAG 1 cut(s) 1025
Bsh1236I CGCG 1 cut(s) 1293
BshFI GGCC 3 cut(s) 623, 1312, 1422
BshNI GGYRCC 2 cut(s) 241, 1256
BslI CCNNNNNNNGG 4 cut(s) 499, 863, 1266, 1544
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 3 cut(s) 623, 1312, 1422
Bsp1286I GDGCHC 2 cut(s) 244, 1408
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 3 cut(s) 134, 189, 1291
BspANI GGCC 3 cut(s) 623, 1312, 1422
BspCNI CTCAG 3 cut(s) 39, 1006, 1063
BspFNI CGCG 1 cut(s) 1293
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 4 cut(s) 243, 1258, 1311, 1556
BspMAI CTGCAG 1 cut(s) 1012
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 4 cut(s) 326, 846, 1101, 1277
BspT107I GGYRCC 2 cut(s) 241, 1256
BsrDI GCAATG 2 cut(s) 159, 474
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 37, 110, 369, 857
BssMI GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
BssNAI GTATAC 1 cut(s) 1374
BssT1I CCWWGG 2 cut(s) 37, 369
Bst1107I GTATAC 1 cut(s) 1374
Bst2UI CCWGG 2 cut(s) 1018, 1251
Bst4CI ACNGT 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 226
BstC8I GCNNGC 6 cut(s) 311, 411, 652, 988, 1115, 1212
BstDEI CTNAG 4 cut(s) 47, 353, 993, 1071
BstDSI CCRYGG 3 cut(s) 110, 369, 857
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 1 cut(s) 868
BstFNI CGCG 1 cut(s) 1293
BstH2I RGCGCY 1 cut(s) 993
BstHHI GCGC 2 cut(s) 992, 1070
BstKTI GATC 7 cut(s) 104, 334, 359, 474, 841, 1096, 1272
BstMBI GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
BstMWI GCNNNNNNNGC 7 cut(s) 48, 115, 229, 358, 585, 1211, 1246
BstNI CCWGG 2 cut(s) 1018, 1251
BstNSI RCATGY 4 cut(s) 227, 295, 514, 928
BstSCI CCNGG 2 cut(s) 1016, 1249
BstSFI CTRYAG 1 cut(s) 1008
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 17, 148, 318, 895
BstX2I RGATCY 1 cut(s) 1269
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 1269
BstZ17I GTATAC 1 cut(s) 1374
BsuRI GGCC 3 cut(s) 623, 1312, 1422
BtgI CCRYGG 3 cut(s) 110, 369, 857
BtsCI GGATG 1 cut(s) 868
BtsI GCAGTG 2 cut(s) 999, 1037
BtsIMutI CAGTG 3 cut(s) 111, 999, 1037
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 6 cut(s) 311, 411, 652, 988, 1115, 1212
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 2 cut(s) 992, 1070
Cfr13I GGNCC 5 cut(s) 131, 183, 880, 1020, 1310
CseI GACGC 3 cut(s) 821, 1166, 1447
Csp6I GTAC 4 cut(s) 227, 251, 295, 979
CviAII CATG 8 cut(s) 224, 292, 370, 511, 555, 925, 1097, 1544
CviQI GTAC 4 cut(s) 227, 251, 295, 979
DdeI CTNAG 4 cut(s) 47, 353, 993, 1071
DpnI GATC 7 cut(s) 103, 333, 358, 473, 840, 1095, 1271
DpnII GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
DraI TTTAAA 1 cut(s) 798
Eco130I CCWWGG 2 cut(s) 37, 369
Eco24I GRGCYC 1 cut(s) 1408
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 4 cut(s) 131, 183, 880, 1020
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 1016, 1249
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 37, 369
EcoT38I GRGCYC 1 cut(s) 1408
ErhI CCWWGG 2 cut(s) 37, 369
FaeI CATG 8 cut(s) 227, 295, 373, 514, 558, 928, 1100, 1547
FatI CATG 8 cut(s) 223, 291, 369, 510, 554, 924, 1096, 1543
FauNDI CATATG 2 cut(s) 1265, 1384
FblI GTMKAC 2 cut(s) 382, 1373
Fnu4HI GCNGC 4 cut(s) 6, 137, 307, 909
FokI GGATG 1 cut(s) 875
FriOI GRGCYC 1 cut(s) 1408
Fsp4HI GCNGC 4 cut(s) 6, 137, 307, 909
FspBI CTAG 3 cut(s) 90, 630, 1232
GlaI GCGC 2 cut(s) 991, 1069
GluI GCNGC 4 cut(s) 6, 137, 307, 909
HaeII RGCGCY 1 cut(s) 993
HaeIII GGCC 3 cut(s) 623, 1312, 1422
HgaI GACGC 3 cut(s) 821, 1166, 1447
HhaI GCGC 2 cut(s) 992, 1070
Hin1II CATG 8 cut(s) 227, 295, 373, 514, 558, 928, 1100, 1547
Hin6I GCGC 2 cut(s) 990, 1068
HinP1I GCGC 2 cut(s) 990, 1068
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 7 cut(s) 97, 178, 280, 581, 759, 1162, 1171
HphI GGTGA 5 cut(s) 190, 434, 538, 1171, 1496
Hpy166II GTNNAC 6 cut(s) 57, 186, 253, 383, 811, 1374
Hpy188I TCNGA 8 cut(s) 48, 128, 155, 522, 724, 946, 996, 1497
Hpy188III TCNNGA 5 cut(s) 630, 645, 707, 1097, 1166
Hpy8I GTNNAC 6 cut(s) 57, 186, 253, 383, 811, 1374
HpyAV CCTTC 3 cut(s) 87, 150, 613
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 1060
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 115, 229, 358, 585, 1211, 1246
HpyF3I CTNAG 4 cut(s) 47, 353, 993, 1071
HpySE526I ACGT 1 cut(s) 1060
Hsp92II CATG 8 cut(s) 227, 295, 373, 514, 558, 928, 1100, 1547
HspAI GCGC 2 cut(s) 990, 1068
Kzo9I GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
LmnI GCTCC 2 cut(s) 1237, 1560
Lsp1109I GCAGC 4 cut(s) 17, 148, 318, 895
LweI GCATC 5 cut(s) 132, 218, 878, 1017, 1306
MaeI CTAG 3 cut(s) 90, 630, 1232
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 4 cut(s) 885, 920, 1035, 1527
MalI GATC 7 cut(s) 103, 333, 358, 473, 840, 1095, 1271
MboI GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
MboII GAAGA 8 cut(s) 25, 187, 253, 481, 722, 796, 1264, 1459
MflI RGATCY 1 cut(s) 1269
MhlI GDGCHC 2 cut(s) 244, 1408
MlyI GAGTC 2 cut(s) 289, 1180
MnlI CCTC 8 cut(s) 122, 256, 459, 604, 799, 828, 1112, 1247
MseI TTAA 8 cut(s) 69, 341, 504, 549, 797, 825, 939, 1329
MspR9I CCNGG 2 cut(s) 1018, 1251
Mva1269I GAATGC 1 cut(s) 42
MvaI CCWGG 2 cut(s) 1018, 1251
MvnI CGCG 1 cut(s) 1293
MwoI GCNNNNNNNGC 7 cut(s) 48, 115, 229, 358, 585, 1211, 1246
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 2 cut(s) 1265, 1384
NdeII GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
NlaIII CATG 8 cut(s) 227, 295, 373, 514, 558, 928, 1100, 1547
NlaIV GGNNCC 4 cut(s) 243, 1258, 1311, 1556
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 920, 1035
NspI RCATGY 4 cut(s) 227, 295, 514, 928
PagI TCATGA 1 cut(s) 1096
PaqCI CACCTGC 1 cut(s) 410
PciI ACATGT 2 cut(s) 291, 510
PctI GAATGC 1 cut(s) 42
PfeI GAWTC 5 cut(s) 97, 178, 581, 759, 1162
PflMI CCANNNNNTGG 2 cut(s) 863, 1266
PkrI GCNGC 4 cut(s) 7, 138, 308, 910
PleI GAGTC 2 cut(s) 288, 1179
PpsI GAGTC 2 cut(s) 288, 1179
PpuMI RGGWCCY 1 cut(s) 880
PscI ACATGT 2 cut(s) 291, 510
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 2 cut(s) 1016, 1249
PspGI CCWGG 2 cut(s) 1016, 1249
PspN4I GGNNCC 4 cut(s) 243, 1258, 1311, 1556
PspPI GGNCC 5 cut(s) 131, 183, 880, 1020, 1310
PspPPI RGGWCCY 1 cut(s) 880
PstI CTGCAG 1 cut(s) 1012
PsuI RGATCY 1 cut(s) 1269
RsaI GTAC 4 cut(s) 228, 252, 296, 980
RsaNI GTAC 4 cut(s) 227, 251, 295, 979
SaqAI TTAA 8 cut(s) 69, 341, 504, 549, 797, 825, 939, 1329
SatI GCNGC 4 cut(s) 6, 137, 307, 909
Sau3AI GATC 7 cut(s) 101, 331, 356, 471, 838, 1093, 1269
Sau96I GGNCC 5 cut(s) 131, 183, 880, 1020, 1310
SchI GAGTC 2 cut(s) 289, 1180
ScrFI CCNGG 2 cut(s) 1018, 1251
SduI GDGCHC 2 cut(s) 244, 1408
SfaNI GCATC 5 cut(s) 132, 218, 878, 1017, 1306
SfcI CTRYAG 1 cut(s) 1008
SinI GGWCC 4 cut(s) 131, 183, 880, 1020
SmlI CTYRAG 1 cut(s) 754
SmoI CTYRAG 1 cut(s) 754
SsiI CCGC 3 cut(s) 134, 189, 1291
SspMI CTAG 3 cut(s) 90, 630, 1232
StyD4I CCNGG 2 cut(s) 1016, 1249
StyI CCWWGG 2 cut(s) 37, 369
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 3 cut(s) 100, 212, 1408
TaqII GACCGA 1 cut(s) 171
TatI WGTACW 3 cut(s) 226, 294, 978
TfiI GAWTC 5 cut(s) 97, 178, 581, 759, 1162
Tru1I TTAA 8 cut(s) 69, 341, 504, 549, 797, 825, 939, 1329
Tru9I TTAA 8 cut(s) 69, 341, 504, 549, 797, 825, 939, 1329
TscAI CASTG 3 cut(s) 111, 1006, 1044
TseFI GTSAC 2 cut(s) 920, 1035
TseI GCWGC 4 cut(s) 5, 136, 306, 908
Tsp45I GTSAC 2 cut(s) 920, 1035
TspDTI ATGAA 4 cut(s) 132, 253, 410, 1460
TspGWI ACGGA 2 cut(s) 238, 459
TspRI CASTG 3 cut(s) 111, 1006, 1044
Van91I CCANNNNNTGG 2 cut(s) 863, 1266
VpaK11BI GGWCC 4 cut(s) 131, 183, 880, 1020
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 2 cut(s) 485, 1410
XbaI TCTAGA 1 cut(s) 629
XceI RCATGY 4 cut(s) 227, 295, 514, 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmiI GTMKAC 2 cut(s) 382, 1373
XspI CTAG 3 cut(s) 90, 630, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.