Rorug01G0374300

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000980
Physical Location & Seq
Reverse (-)
48849494 .. 48852568
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0374300.1

Sequence Viewer

Length: 831 bp
ATGAGTTTTGTTTTCCGGGGGACAAGAGGCGATATTGAGAGTGGGTTTCCCGAATTCATACCAGAGCGGCGCGCAATGCGTGTTCATACAGCACGCTCTGTCAATTCCAACTCACTTGCTTTTCTCGTCACAGTTCTACTGCTATTTATGATACTGAACTCGCACCAAATGTCTCCTAATTTCCTGCTGTGGCTAGTCCTCGGGGTCTTTTTGATGGCTACAACGTTAAGGATGTATGCAACCTGTCAACAGCTGCAAGCTCAAGCACAGGCTCATGCAGCAGCCGCCAGTGGCCTTCTTGGTCATACTGAATTGCGGTTGCATATGCCACCATCCATAGCACTAGCAACAAGAGGCCGCCTTCAAGGTCTCAGGCTTCAGCTTGCACTTCTTGACCGTGAATTTGATGACCTAGATTATGAAACTTTGCGAGCTCTAGACTCCGACAATGTTCCATCAGCATCTTCTATGAGTGAGGAAGAGATAAATGCTCTTCCTGTCCACAAATATAAAGTTGCGGGTCCTCAAAATGGTACCTCATTGACGCAACAAGCTTCAACCTCAGTACCTGCTGAGCAGAAGAAGCAAGAAACTGCTGATGTTGGGAGCACCAAAGCCATTGAAGATGAACTGACCTGTAGTGTCTGCTTGGAGCAAGTTAACACGGGGGAGCTCATCCGCAGCCTTCCTTGCTTGCATCAGTTTCATGCTAGTTGCATTGACCCTTGGCTAAGGCAACAGGGAACATGCCCAGTGTGTAAATACAGAGCAGGTTCTGGATGGCATGAAAACGGACAAGGTGCAGCAATGGATGCTTCTTACATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

276

Amino Acids

30.36

Weight (kDa)

5.98

Isoelectric Point (pI)

58.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 212 - 254 1.7e-13 Ring finger domain
zf-C3HC4_2 PF13923 213 - 253 4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 213 - 240 2.5e-08 RING-like zinc finger
zf-C3HC4 PF00097 213 - 253 2.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 229 - 254 2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 577, 761
Acc65I GGTACC 1 cut(s) 533
AccB1I GGYRCC 1 cut(s) 533
AccBSI CCGCTC 1 cut(s) 67
AccII CGCG 1 cut(s) 72
AciI CCGC 6 cut(s) 67, 285, 316, 358, 518, 679
AclI AACGTT 1 cut(s) 224
AcsI RAATTY 2 cut(s) 53, 401
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 2 cut(s) 535, 567
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 3 cut(s) 365, 558, 623
AluBI AGCT 6 cut(s) 253, 260, 382, 434, 554, 673
AluI AGCT 6 cut(s) 253, 260, 382, 434, 554, 673
Alw21I GWGCWC 3 cut(s) 436, 611, 675
Alw26I GTCTC 2 cut(s) 177, 374
AlwNI CAGNNNCTG 2 cut(s) 569, 776
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 2 cut(s) 292, 355
ApeKI GCWGC 5 cut(s) 253, 278, 281, 681, 803
ApoI RAATTY 2 cut(s) 53, 401
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp718I GGTACC 1 cut(s) 533
AspLEI GCGC 2 cut(s) 72, 74
AspS9I GGNCC 1 cut(s) 521
AsuC2I CCSGG 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 521
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 2 cut(s) 436, 675
Bbv12I GWGCWC 3 cut(s) 436, 611, 675
BbvI GCAGC 5 cut(s) 240, 290, 293, 693, 815
BccI CCATC 4 cut(s) 208, 340, 463, 774
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 2 cut(s) 177, 374
BfaI CTAG 5 cut(s) 194, 344, 413, 437, 711
BfmI CTRYAG 1 cut(s) 637
BfuAI ACCTGC 2 cut(s) 577, 761
BisI GCNGC 8 cut(s) 68, 254, 279, 282, 285, 358, 682, 804
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 8 cut(s) 69, 255, 280, 283, 286, 359, 683, 805
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 521
BmiI GGNNCC 2 cut(s) 522, 535
BmrFI CCNGG 1 cut(s) 17
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 3 cut(s) 470, 706, 802
BmuI ACTGGG 1 cut(s) 746
Bpu10I CCTNAGC 1 cut(s) 731
Bpu1102I GCTNAGC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 16, 199, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 288, 752
Bse3DI GCAATG 2 cut(s) 81, 813
BseDI CCNNGG 3 cut(s) 16, 199, 725
BseGI GGATG 5 cut(s) 237, 332, 675, 785, 817
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 2 cut(s) 81, 813
BseMII CTCAG 3 cut(s) 385, 564, 576
BseNI ACTGG 2 cut(s) 288, 752
BsePI GCGCGC 1 cut(s) 70
BseXI GCAGC 5 cut(s) 240, 290, 293, 693, 815
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 294, 357
BshNI GGYRCC 1 cut(s) 533
BsiHKAI GWGCWC 3 cut(s) 436, 611, 675
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 177, 374
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 2 cut(s) 294, 357
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 3 cut(s) 436, 611, 675
Bsp1720I GCTNAGC 1 cut(s) 573
BspACI CCGC 6 cut(s) 67, 285, 316, 358, 518, 679
BspANI GGCC 2 cut(s) 294, 357
BspCNI CTCAG 3 cut(s) 384, 565, 575
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 522, 535
BspMI ACCTGC 2 cut(s) 577, 761
BspQI GCTCTTC 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 81, 813
BsrI ACTGG 2 cut(s) 288, 752
BssECI CCNNGG 3 cut(s) 16, 199, 725
BssHII GCGCGC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 725
Bst4CI ACNGT 2 cut(s) 133, 398
Bst6I CTCTTC 2 cut(s) 474, 498
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 6 cut(s) 72, 94, 258, 384, 432, 695
BstDEI CTNAG 4 cut(s) 371, 562, 573, 731
BstF5I GGATG 5 cut(s) 237, 332, 675, 785, 817
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 2 cut(s) 72, 74
BstMAI GTCTC 2 cut(s) 177, 374
BstMWI GCNNNNNNNGC 6 cut(s) 76, 278, 284, 583, 690, 812
BstNSI RCATGY 1 cut(s) 750
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 637
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 5 cut(s) 240, 290, 293, 693, 815
BsuRI GGCC 2 cut(s) 294, 357
BtsCI GGATG 5 cut(s) 237, 332, 675, 785, 817
BtsIMutI CAGTG 2 cut(s) 295, 759
BveI ACCTGC 2 cut(s) 577, 761
Cac8I GCNNGC 6 cut(s) 72, 94, 258, 384, 432, 695
CaiI CAGNNNCTG 2 cut(s) 569, 776
CfoI GCGC 2 cut(s) 72, 74
Cfr13I GGNCC 1 cut(s) 521
CseI GACGC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 534, 566
CviAII CATG 5 cut(s) 275, 707, 747, 785, 823
CviQI GTAC 2 cut(s) 534, 566
DdeI CTNAG 4 cut(s) 371, 562, 573, 731
Eam1104I CTCTTC 2 cut(s) 474, 498
EarI CTCTTC 2 cut(s) 474, 498
Ecl136II GAGCTC 2 cut(s) 434, 673
Eco130I CCWWGG 1 cut(s) 725
Eco24I GRGCYC 2 cut(s) 436, 675
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 521
Eco53kI GAGCTC 2 cut(s) 434, 673
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 2 cut(s) 434, 673
EcoO109I RGGNCCY 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 725
EcoT38I GRGCYC 2 cut(s) 436, 675
ErhI CCWWGG 1 cut(s) 725
FaeI CATG 5 cut(s) 278, 710, 750, 788, 826
FaqI GGGAC 1 cut(s) 34
FatI CATG 5 cut(s) 274, 706, 746, 784, 822
FauI CCCGC 1 cut(s) 511
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 8 cut(s) 68, 254, 279, 282, 285, 358, 682, 804
FokI GGATG 5 cut(s) 244, 319, 662, 792, 824
FriOI GRGCYC 2 cut(s) 436, 675
Fsp4HI GCNGC 8 cut(s) 68, 254, 279, 282, 285, 358, 682, 804
FspBI CTAG 5 cut(s) 194, 344, 413, 437, 711
GlaI GCGC 2 cut(s) 71, 73
GluI GCNGC 8 cut(s) 68, 254, 279, 282, 285, 358, 682, 804
HaeIII GGCC 2 cut(s) 294, 357
HapII CCGG 1 cut(s) 16
HgaI GACGC 1 cut(s) 553
HhaI GCGC 2 cut(s) 72, 74
Hin1II CATG 5 cut(s) 278, 710, 750, 788, 826
Hin6I GCGC 2 cut(s) 70, 72
HinP1I GCGC 2 cut(s) 70, 72
HincII GTYRAC 2 cut(s) 248, 661
HindII GTYRAC 2 cut(s) 248, 661
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 1 cut(s) 440
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 248, 502, 661
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 4 cut(s) 50, 392, 437, 777
Hpy8I GTNNAC 3 cut(s) 248, 502, 661
HpyAV CCTTC 3 cut(s) 305, 371, 695
HpyCH4III ACNGT 2 cut(s) 133, 398
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 8 cut(s) 239, 256, 278, 322, 386, 697, 717, 803
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 278, 284, 583, 690, 812
HpyF3I CTNAG 4 cut(s) 371, 562, 573, 731
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 5 cut(s) 278, 710, 750, 788, 826
HspAI GCGC 2 cut(s) 70, 72
KpnI GGTACC 1 cut(s) 537
KspAI GTTAAC 1 cut(s) 661
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 3 cut(s) 606, 652, 670
Lsp1109I GCAGC 5 cut(s) 240, 290, 293, 693, 815
LweI GCATC 3 cut(s) 470, 706, 802
MaeI CTAG 5 cut(s) 194, 344, 413, 437, 711
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 1 cut(s) 127
MbiI CCGCTC 1 cut(s) 67
MboII GAAGA 5 cut(s) 456, 485, 491, 592, 635
MhlI GDGCHC 3 cut(s) 436, 611, 675
MluCI AATT 5 cut(s) 53, 103, 178, 311, 401
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 2 cut(s) 132, 468
MnlI CCTC 7 cut(s) 20, 209, 347, 469, 534, 547, 571
MseI TTAA 2 cut(s) 227, 660
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 17
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 6 cut(s) 76, 278, 284, 583, 690, 812
NciI CCSGG 1 cut(s) 17
NdeI CATATG 1 cut(s) 324
NlaIII CATG 5 cut(s) 278, 710, 750, 788, 826
NlaIV GGNNCC 2 cut(s) 522, 535
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 750
PauI GCGCGC 1 cut(s) 70
PciSI GCTCTTC 1 cut(s) 498
PkrI GCNGC 8 cut(s) 69, 255, 280, 283, 286, 359, 683, 805
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 521
Psp124BI GAGCTC 2 cut(s) 436, 675
Psp1406I AACGTT 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 521
PspN4I GGNNCC 2 cut(s) 522, 535
PspPI GGNCC 1 cut(s) 521
PspPPI RGGWCCY 1 cut(s) 521
PstNI CAGNNNCTG 2 cut(s) 569, 776
PteI GCGCGC 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 2 cut(s) 535, 567
RsaNI GTAC 2 cut(s) 534, 566
SacI GAGCTC 2 cut(s) 436, 675
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 2 cut(s) 227, 660
SatI GCNGC 8 cut(s) 68, 254, 279, 282, 285, 358, 682, 804
Sau96I GGNCC 1 cut(s) 521
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 3 cut(s) 436, 611, 675
SfaNI GCATC 3 cut(s) 470, 706, 802
SfcI CTRYAG 1 cut(s) 637
SinI GGWCC 1 cut(s) 521
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 5 cut(s) 53, 103, 178, 311, 401
SsiI CCGC 6 cut(s) 67, 285, 316, 358, 518, 679
SspMI CTAG 5 cut(s) 194, 344, 413, 437, 711
SstI GAGCTC 2 cut(s) 436, 675
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 725
TaaI ACNGT 2 cut(s) 133, 398
TaiI ACGT 1 cut(s) 227
TasI AATT 5 cut(s) 53, 103, 178, 311, 401
TauI GCSGC 3 cut(s) 70, 287, 360
Tru1I TTAA 2 cut(s) 227, 660
Tru9I TTAA 2 cut(s) 227, 660
TscAI CASTG 2 cut(s) 295, 759
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 5 cut(s) 253, 278, 281, 681, 803
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 6 cut(s) 46, 74, 435, 642, 695, 801
TspGWI ACGGA 1 cut(s) 807
TspRI CASTG 2 cut(s) 295, 759
VpaK11BI GGWCC 1 cut(s) 521
XapI RAATTY 2 cut(s) 53, 401
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 1 cut(s) 750
XspI CTAG 5 cut(s) 194, 344, 413, 437, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.