Rorug03G0037800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000980
Physical Location & Seq
Reverse (-)
2963641 .. 2972878
9238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0037800.1

Sequence Viewer

Length: 2919 bp
ATGGGCGTCCCCGCGTTTTACCGCTGGCTGGCGGATCGGTACCCGCTCTCGATCGTCGACGTGGTGGAGGAACACCCCAGGGAGGACCCGAACGGCGTGCCGAGACCAATCGACGTCTCGAGGCCAAACCCTAACGGCTACGAGTTCGATAACCTTTACCTCGACATGAACGGCATTATACATCCCTGTTTTCATCCCGACGGCCGACCTGCCCCTGCTACTTATCAGGACGTATTCAAGTCAATATTTGACTACATTGATCATCTCTTCACGCTGGTGCGCCCAAGAAAGCTTCTTTTTATGGCGATTGATGGAGTAGCACCAAGAGCTAAAATGAATCAGCAACGCTCTCGACGTTTCAGAGCTGCAAAAGATGCGGCTGAGGCGGAAGCTGAAGAGGAAAGACTAAAGAAAGAATTTGAGATGGAGGGGACATCATTGGTTTCTAAAGAGAAACCTGAAACTTCAGATTCAAATGTTATTACTCCAGGCACTCAATTTATGGCGGTGCTCTCAGTAGCACTTCAATATTATATACAATTTAAATTGAACAACGACCGTGGTTGGCGGTCTACAAAGGTTATTCTTTCTGATGCCAACGTTCCTGGTGAGGGAGAGCACAAAATAATGTCATACATACGATCGCAACGCAATCTTTTGGGTTCTGATCCTAACACCCGGCATTGTCTTTATGGTCTGGATGCAGATCTAATTATGCTTGCTTTAGCTACACATGAGGTTCACTTCTCAATATTAAGAGAGGTGATTACTCTTCCAGGTCAACAGGAAAAATGTTTTCTTTGTGGACAAGTTGGTCATCTGGCAGCTGAATGTCGTGGTGGAGATGATGGTGCGGCCGATGAAACTCCAGTTCACAAGAAGAAATATCAGTTCCTCAAAATCTGGGTGTTGCGTGAATATTTGGAATACGAGCTGGACATACCCAATCCTCCTTTTCAGCTTAATTTTGAAAGGATAGTGGATGATTTTGTGTTTATGTGCTTTTTTGTTGGCAATGATTTTCTTCCACATATGCCAACATTGGAGATCAGAGAGGGTGCAATTAATTTATTGATGCATATTTATAGAAGAGAATTTTCTGCTATGGGTGGCTATCTCACGGATGCGGGTGAGGTATTGTTAGATAGAGTAGAGCATTTTATCCAGTCTGTTGCTGTCTATGAGCGCCAAATTTTTCAAAAGCGGGCTCGAATTCAGCAGGCTTTTGAGAATAATGAAGAGAGACAGAGAAATAGAAGAGAGGCTTGTGAAGAGCTACCGGCTCCAGTGGTAGAAAAGGTGGTGGACAAGGTTAAATTGGGGGAGCCGGGATATACAGAAAGGTATTATGCTGAGAAGTTTCAAGTATCAAAGCCAGAGGAGATTGAAAAAGTTAAAAAAGACTTGGTTCTAAAATATGTAGAAGGCTTATGTTGGGTCTGCCGATATTACTACCAAGGGGTCTGCTCATGGCAATGGTATTATCCATACCATTATGCTCCTTTTGCTTCTGATCTAAAAGATCTAGATGAGTTGGAAATAACATTTTTCTTGGGAGAGCCATTTAAACCCTTTGATCAGCTCATGGGAACCTTACCATCTGCAAGTTGTAGTGCTCTTCCAGAAAAATACAGAAATTTGATGACTGATCCGTCATCTCCCATTTGTGATTTCTACCCATCTGATTTTGAGATTGACATGAATGGCAAGCGCTTTGCGTGGCAGGGCATTGCCAAATTGCCATTCATTGATGAGAAGAAATTGCTTGCTCAGACAAGGAAGCTTGAAAGCACTTTAACGGAGGAAGAGAAAGCTCGAAATAGCGTGATGCTTGATTTGCTTTATGTCTATCCTTCTCATCCTTTGGCGCCGGATATTATTTTGTACTACCATTTATATTATCAGAGTCCCCCACATGAAAGATTTTTATATCCGATAAATACTAATCTTAGTGGTGGAATGAATGGATTCCTTTGGTTATCTGAGAGGAATGGCTTTAGAAACGTAATCCCTTCCCCTGTGCATGGTTTTCCCAACATAGACTATAACCAAGTCTTAAATGTTACATATCTTAGCCCTGCAGCTCAAAAACACATCTCAGAACCTGCCAAGGGTGTGATCATGCCCAGAAAGGTTGTAAAACCTATGGATATCAAACCTCCCCCTGCGTTATGGCATGAAGACAATGGTCCCCGACGCTACCAAAACAGAGATAGGCCACAAATACCTGGAGCAATTGCTGGAACTGTGTTGGGAGAAGCAGCTCATCGACTTGTCAAGAACACACTAAATCTTAGACCTAGTAATCCATCCTCTGGATTTTGGGATCAATCGCAGCCTTTACGAACTTCTTCTGGAAACTACATCGGCAACTATCCCGTCAACAGACCAAGACCAGCTGGGCCATCTGGGTATGAAAGGGGTTTCCGTGAAGAACCAAATTATGGAAATTCCTTTAATCCTCAGGGCATCATGGCTAGGCCCAGAGCTCCTTACTCAAATGGTGTGCAGGGTGACAGACAGAGTTTTAGGACACAAGAAAGAACACAGTATCAAGAGCAGAGTTTTAGAGGACAAGAAAGAATACAATATCAAGAACAGTACCGCAACATGAGAACTGGGATGTCTAGTTTAACAATGGAAGGAAGTGTGAGAACCAGGTCTCCTGTAGTGGCGCAACCAGGGATGCCAAATTCAGGATATCCCACAAACCCACATCAGCAGTTTGTGCAGAATATGGGCGCTCTTCCAGCGCCACCTACAAAGTGGATCAACAAAGAATCGAGTGCCAATGGTGGGATTTATGGTAGGCATCAGGAAACCGGATATGGCGGACCATATGAGCAGCAGGTGAAAAAGGTCTATCAGGTAAAGACACGTCCTCCTCAGGACTTGTCAAACTCTGGAAACCAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

972

Amino Acids

111.56

Weight (kDa)

6.57

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XRN_N PF03159 1 - 254 1.9e-99 XRN 5'-3' exonuclease N-terminus
XRN_M PF17846 321 - 422 4.5e-43 Xrn1 helical domain
XRN_M PF17846 432 - 718 4.8e-116 Xrn1 helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2839
AasI GACNNNNNNGTC 2 cut(s) 813, 1651
AatII GACGTC 1 cut(s) 117
Acc36I ACCTGC 3 cut(s) 217, 2113, 2839
Acc65I GGTACC 1 cut(s) 39
AccB1I GGYRCC 2 cut(s) 39, 1867
AccB7I CCANNNNNTGG 2 cut(s) 2315, 2444
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 2 cut(s) 57, 572
AccII CGCG 1 cut(s) 14
AclI AACGTT 1 cut(s) 600
AclWI GGATC 5 cut(s) 42, 662, 1643, 2334, 2777
AcoI YGGCCR 2 cut(s) 202, 855
AcsI RAATTY 7 cut(s) 416, 1094, 1191, 1212, 1636, 2449, 2692
AcuI CTGAAG 2 cut(s) 414, 450
AcyI GRCGYC 3 cut(s) 6, 114, 1868
AfaI GTAC 3 cut(s) 41, 1886, 2603
AfeI AGCGCT 1 cut(s) 1712
AfiI CCNNNNNNNGG 9 cut(s) 28, 82, 611, 2024, 2111, 2315, 2444, 2696, 2796
AflIII ACRYGT 1 cut(s) 2876
AgsI TTSAA 9 cut(s) 238, 474, 527, 550, 971, 1199, 1364, 1388, 1787
AjiI CACGTC 2 cut(s) 61, 2879
AjnI CCWGG 8 cut(s) 77, 487, 604, 775, 2227, 2657, 2680, 2910
AjuI GAANNNNNNNTTGG 2 cut(s) 1727, 1759
AleI CACNNNNGTG 1 cut(s) 275
AloI GAACNNNNNNTCC 2 cut(s) 2647, 2679
Alw21I GWGCWC 4 cut(s) 513, 621, 1618, 2491
Alw26I GTCTC 4 cut(s) 97, 121, 1237, 2667
AlwI GGATC 5 cut(s) 42, 662, 1643, 2334, 2777
AlwNI CAGNNNCTG 1 cut(s) 2105
Ama87I CYCGRG 1 cut(s) 118
Aor51HI AGCGCT 1 cut(s) 1712
AoxI GGCC 6 cut(s) 122, 202, 855, 2216, 2402, 2480
ApeKI GCWGC 6 cut(s) 365, 824, 2081, 2261, 2335, 2845
ApoI RAATTY 7 cut(s) 416, 1094, 1191, 1212, 1636, 2449, 2692
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 2 cut(s) 1967, 2350
Asp718I GGTACC 1 cut(s) 39
AspLEI GCGC 7 cut(s) 282, 1188, 1713, 1870, 2677, 2744, 2755
AspS9I GGNCC 5 cut(s) 85, 2189, 2402, 2481, 2834
AsuC2I CCSGG 2 cut(s) 679, 1329
AsuHPI GGTGA 5 cut(s) 620, 775, 1142, 2525, 2863
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 3 cut(s) 85, 2189, 2834
AxyI CCTNAGG 2 cut(s) 2463, 2886
BanI GGYRCC 2 cut(s) 39, 1867
BanII GRGCYC 2 cut(s) 1210, 2491
BbsI GAAGAC 1 cut(s) 2187
Bbv12I GWGCWC 4 cut(s) 513, 621, 1618, 2491
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 6 cut(s) 352, 836, 2093, 2273, 2347, 2857
BccI CCATC 7 cut(s) 305, 418, 842, 1606, 1687, 2317, 2413
BceAI ACGGC 4 cut(s) 109, 151, 187, 217
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 332, 366
BciT130I CCWGG 8 cut(s) 79, 489, 606, 777, 2229, 2659, 2682, 2912
BclI TGATCA 3 cut(s) 259, 1576, 2118
BcnI CCSGG 2 cut(s) 679, 1329
BcoDI GTCTC 4 cut(s) 97, 121, 1237, 2667
BfaI CTAG 4 cut(s) 1526, 2301, 2478, 2628
BfmI CTRYAG 2 cut(s) 2079, 2667
BfoI RGCGCY 5 cut(s) 1189, 1714, 1871, 2745, 2756
BfuAI ACCTGC 3 cut(s) 217, 2113, 2839
BglII AGATCT 2 cut(s) 706, 1522
BisI GCNGC 8 cut(s) 366, 378, 825, 855, 2082, 2262, 2336, 2846
BlsI GCNGC 8 cut(s) 367, 379, 826, 856, 2083, 2263, 2337, 2847
Bme18I GGWCC 3 cut(s) 85, 2189, 2834
BmeT110I CYCGRG 1 cut(s) 118
BmgBI CACGTC 2 cut(s) 61, 2879
BmgT120I GGNCC 5 cut(s) 85, 2189, 2402, 2481, 2834
BmiI GGNNCC 7 cut(s) 41, 87, 1284, 1326, 1591, 1869, 2191
BmrI ACTGGG 1 cut(s) 2628
BmsI GCATC 9 cut(s) 364, 583, 691, 1065, 1114, 1818, 2478, 2676, 2821
BmuI ACTGGG 1 cut(s) 2628
BoxI GACNNNNGTC 1 cut(s) 2187
BpiI GAAGAC 1 cut(s) 2187
BpmI CTGGAG 4 cut(s) 471, 852, 1269, 2250
Bpu10I CCTNAGC 1 cut(s) 381
BpuMI CCSGG 2 cut(s) 679, 1329
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 3 cut(s) 6, 114, 1868
BsaI GGTCTC 2 cut(s) 97, 2667
BsaJI CCNNGG 6 cut(s) 77, 78, 559, 1456, 2109, 2681
BsaWI WCCGGW 1 cut(s) 2822
BsaXI ACNNNNNCTCC 4 cut(s) 2647, 2677, 2866, 2896
Bsc4I CCNNNNNNNGG 9 cut(s) 28, 82, 611, 2024, 2111, 2315, 2444, 2696, 2796
Bse118I RCCGGY 1 cut(s) 1279
Bse1I ACTGG 4 cut(s) 869, 1165, 1286, 2623
Bse21I CCTNAGG 2 cut(s) 2463, 2886
Bse3DI GCAATG 3 cut(s) 1021, 1481, 1728
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 8 cut(s) 79, 489, 606, 777, 2229, 2659, 2682, 2912
BseDI CCNNGG 6 cut(s) 77, 78, 559, 1456, 2109, 2681
BseGI GGATG 9 cut(s) 181, 193, 706, 988, 1129, 1858, 2309, 2628, 2691
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 9 cut(s) 28, 82, 611, 2024, 2111, 2315, 2444, 2696, 2796
BseMI GCAATG 3 cut(s) 1021, 1481, 1728
BseMII CTCAG 8 cut(s) 372, 528, 1344, 1784, 1974, 2112, 2477, 2900
BseNI ACTGG 4 cut(s) 869, 1165, 1286, 2623
BseRI GAGGAG 2 cut(s) 1394, 2874
BseX3I CGGCCG 2 cut(s) 202, 855
BseXI GCAGC 6 cut(s) 352, 836, 2093, 2273, 2347, 2857
BseYI CCCAGC 1 cut(s) 2399
BsgI GTGCAG 2 cut(s) 2528, 2750
Bsh1236I CGCG 1 cut(s) 14
Bsh1285I CGRYCG 5 cut(s) 54, 205, 559, 644, 858
BshFI GGCC 6 cut(s) 124, 204, 857, 2218, 2404, 2482
BshNI GGYRCC 2 cut(s) 39, 1867
BsiEI CGRYCG 5 cut(s) 54, 205, 559, 644, 858
BsiHKAI GWGCWC 4 cut(s) 513, 621, 1618, 2491
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 5 cut(s) 679, 1280, 1328, 1871, 2823
BslFI GGGAC 3 cut(s) 445, 1893, 2175
BslI CCNNNNNNNGG 9 cut(s) 28, 82, 611, 2024, 2111, 2315, 2444, 2696, 2796
BsmAI GTCTC 4 cut(s) 97, 121, 1237, 2667
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 445, 1893, 2175
BsnI GGCC 6 cut(s) 124, 204, 857, 2218, 2404, 2482
Bso31I GGTCTC 2 cut(s) 97, 2667
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 5 cut(s) 513, 621, 1210, 1618, 2491
BspANI GGCC 6 cut(s) 124, 204, 857, 2218, 2404, 2482
BspCNI CTCAG 8 cut(s) 373, 527, 1345, 1783, 1975, 2111, 2476, 2899
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 7 cut(s) 41, 87, 1284, 1326, 1591, 1869, 2191
BspMAI CTGCAG 1 cut(s) 2083
BspMI ACCTGC 3 cut(s) 217, 2113, 2839
BspPI GGATC 5 cut(s) 42, 662, 1643, 2334, 2777
BspQI GCTCTTC 3 cut(s) 1266, 1623, 2751
BspT107I GGYRCC 2 cut(s) 39, 1867
BspTNI GGTCTC 2 cut(s) 97, 2667
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 3 cut(s) 1021, 1481, 1728
BsrFI RCCGGY 1 cut(s) 1279
BsrI ACTGG 4 cut(s) 869, 1165, 1286, 2623
BssAI RCCGGY 1 cut(s) 1279
BssECI CCNNGG 6 cut(s) 77, 78, 559, 1456, 2109, 2681
BssNI GRCGYC 3 cut(s) 6, 114, 1868
BssT1I CCWWGG 2 cut(s) 1456, 2109
Bst2UI CCWGG 8 cut(s) 79, 489, 606, 777, 2229, 2659, 2682, 2912
Bst4CI ACNGT 4 cut(s) 560, 2248, 2550, 2601
BstACI GRCGYC 3 cut(s) 6, 114, 1868
BstAPI GCANNNNNTGC 2 cut(s) 374, 2728
BstC8I GCNNGC 8 cut(s) 26, 30, 98, 720, 1206, 1221, 1709, 1767
BstDSI CCRYGG 1 cut(s) 559
BstF5I GGATG 9 cut(s) 181, 193, 706, 988, 1129, 1858, 2309, 2628, 2691
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 5 cut(s) 1189, 1714, 1871, 2745, 2756
BstHHI GCGC 7 cut(s) 282, 1188, 1713, 1870, 2677, 2744, 2755
BstMAI GTCTC 4 cut(s) 97, 121, 1237, 2667
BstMCI CGRYCG 5 cut(s) 54, 205, 559, 644, 858
BstMWI GCNNNNNNNGC 7 cut(s) 326, 374, 383, 1505, 1837, 2728, 2750
BstNI CCWGG 8 cut(s) 79, 489, 606, 777, 2229, 2659, 2682, 2912
BstPAI GACNNNNGTC 1 cut(s) 2187
BstSFI CTRYAG 2 cut(s) 2079, 2667
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 6 cut(s) 352, 836, 2093, 2273, 2347, 2857
BstV2I GAAGAC 1 cut(s) 2187
BstX2I RGATCY 2 cut(s) 706, 1522
BstYI RGATCY 2 cut(s) 706, 1522
BstZI CGGCCG 2 cut(s) 202, 855
Bsu36I CCTNAGG 2 cut(s) 2463, 2886
BsuRI GGCC 6 cut(s) 124, 204, 857, 2218, 2404, 2482
BtgI CCRYGG 1 cut(s) 559
BtrI CACGTC 2 cut(s) 61, 2879
BtsCI GGATG 9 cut(s) 181, 193, 706, 988, 1129, 1858, 2309, 2628, 2691
BtsIMutI CAGTG 1 cut(s) 1293
BveI ACCTGC 3 cut(s) 217, 2113, 2839
Cac8I GCNNGC 8 cut(s) 26, 30, 98, 720, 1206, 1221, 1709, 1767
CaiI CAGNNNCTG 1 cut(s) 2105
CfoI GCGC 7 cut(s) 282, 1188, 1713, 1870, 2677, 2744, 2755
Cfr10I RCCGGY 1 cut(s) 1279
Cfr13I GGNCC 5 cut(s) 85, 2189, 2402, 2481, 2834
CseI GACGC 1 cut(s) 2205
CsiI ACCWGGT 1 cut(s) 2657
Csp6I GTAC 3 cut(s) 40, 1885, 2602
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviQI GTAC 3 cut(s) 40, 1885, 2602
DinI GGCGCC 1 cut(s) 1869
DraI TTTAAA 2 cut(s) 544, 1567
DrdI GACNNNNNNGTC 2 cut(s) 813, 1651
DseDI GACNNNNNNGTC 2 cut(s) 813, 1651
EaeI YGGCCR 2 cut(s) 202, 855
EagI CGGCCG 2 cut(s) 202, 855
EciI GGCGGA 3 cut(s) 47, 401, 2847
Ecl136II GAGCTC 1 cut(s) 2489
EclXI CGGCCG 2 cut(s) 202, 855
Eco130I CCWWGG 2 cut(s) 1456, 2109
Eco24I GRGCYC 2 cut(s) 1210, 2491
Eco31I GGTCTC 2 cut(s) 97, 2667
Eco32I GATATC 2 cut(s) 2152, 2702
Eco47I GGWCC 3 cut(s) 85, 2189, 2834
Eco47III AGCGCT 1 cut(s) 1712
Eco52I CGGCCG 2 cut(s) 202, 855
Eco53kI GAGCTC 1 cut(s) 2489
Eco57I CTGAAG 2 cut(s) 414, 450
Eco81I CCTNAGG 2 cut(s) 2463, 2886
Eco88I CYCGRG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 2489
EcoO109I RGGNCCY 1 cut(s) 85
EcoRI GAATTC 1 cut(s) 1212
EcoRII CCWGG 8 cut(s) 77, 487, 604, 775, 2227, 2657, 2680, 2910
EcoRV GATATC 2 cut(s) 2152, 2702
EcoT14I CCWWGG 2 cut(s) 1456, 2109
EcoT22I ATGCAT 1 cut(s) 1080
EcoT38I GRGCYC 2 cut(s) 1210, 2491
EgeI GGCGCC 1 cut(s) 1869
EheI GGCGCC 1 cut(s) 1869
ErhI CCWWGG 2 cut(s) 1456, 2109
Esp3I CGTCTC 1 cut(s) 121
FalI AAGNNNNNCTT 2 cut(s) 1249, 1281
FaqI GGGAC 3 cut(s) 445, 1893, 2175
FauI CCCGC 4 cut(s) 19, 51, 1120, 1197
FauNDI CATATG 2 cut(s) 1032, 2839
FbaI TGATCA 3 cut(s) 259, 1576, 2118
FblI GTMKAC 2 cut(s) 57, 572
Fnu4HI GCNGC 8 cut(s) 366, 378, 825, 855, 2082, 2262, 2336, 2846
FokI GGATG 9 cut(s) 168, 180, 713, 995, 1136, 1845, 2296, 2635, 2698
FriOI GRGCYC 2 cut(s) 1210, 2491
Fsp4HI GCNGC 8 cut(s) 366, 378, 825, 855, 2082, 2262, 2336, 2846
FspBI CTAG 4 cut(s) 1526, 2301, 2478, 2628
GlaI GCGC 7 cut(s) 281, 1187, 1712, 1869, 2676, 2743, 2754
GluI GCNGC 8 cut(s) 366, 378, 825, 855, 2082, 2262, 2336, 2846
GsaI CCCAGC 1 cut(s) 2403
GsuI CTGGAG 4 cut(s) 471, 852, 1269, 2250
HaeII RGCGCY 5 cut(s) 1189, 1714, 1871, 2745, 2756
HaeIII GGCC 6 cut(s) 124, 204, 857, 2218, 2404, 2482
HapII CCGG 5 cut(s) 679, 1280, 1328, 1871, 2823
HgaI GACGC 1 cut(s) 2205
HhaI GCGC 7 cut(s) 282, 1188, 1713, 1870, 2677, 2744, 2755
Hin1I GRCGYC 3 cut(s) 6, 114, 1868
Hin6I GCGC 7 cut(s) 280, 1186, 1711, 1868, 2675, 2742, 2753
HinP1I GCGC 7 cut(s) 280, 1186, 1711, 1868, 2675, 2742, 2753
HincII GTYRAC 3 cut(s) 58, 782, 2383
HindII GTYRAC 3 cut(s) 58, 782, 2383
HindIII AAGCTT 2 cut(s) 290, 1781
HinfI GANTC 5 cut(s) 337, 470, 1906, 1968, 2780
HpaII CCGG 5 cut(s) 679, 1280, 1328, 1871, 2823
HphI GGTGA 5 cut(s) 620, 775, 1142, 2525, 2863
Hpy166II GTNNAC 8 cut(s) 58, 573, 742, 782, 806, 874, 1306, 2383
Hpy8I GTNNAC 8 cut(s) 58, 573, 742, 782, 806, 874, 1306, 2383
Hpy99I CGWCG 6 cut(s) 59, 62, 116, 203, 357, 2199
HpyAV CCTTC 4 cut(s) 1418, 1863, 2022, 2636
HpyCH4III ACNGT 4 cut(s) 560, 2248, 2550, 2601
HpyCH4IV ACGT 7 cut(s) 60, 114, 231, 355, 600, 2004, 2878
HpyCH4V TGCA 9 cut(s) 368, 704, 1061, 1078, 1604, 2023, 2081, 2509, 2731
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 374, 383, 1505, 1837, 2728, 2750
HpySE526I ACGT 7 cut(s) 60, 114, 231, 355, 600, 2004, 2878
Hsp92I GRCGYC 3 cut(s) 6, 114, 1868
HspAI GCGC 7 cut(s) 280, 1186, 1711, 1868, 2675, 2742, 2753
KasI GGCGCC 1 cut(s) 1867
KpnI GGTACC 1 cut(s) 43
Ksp22I TGATCA 3 cut(s) 259, 1576, 2118
LguI GCTCTTC 3 cut(s) 1266, 1623, 2751
LmnI GCTCC 5 cut(s) 1288, 1324, 1504, 2231, 2494
Lsp1109I GCAGC 6 cut(s) 352, 836, 2093, 2273, 2347, 2857
LweI GCATC 9 cut(s) 364, 583, 691, 1065, 1114, 1818, 2478, 2676, 2821
MabI ACCWGGT 1 cut(s) 2657
MaeI CTAG 4 cut(s) 1526, 2301, 2478, 2628
MaeII ACGT 7 cut(s) 60, 114, 231, 355, 600, 2004, 2878
MaeIII GTNAC 2 cut(s) 2062, 2513
MbiI CCGCTC 1 cut(s) 46
MfeI CAATTG 1 cut(s) 2235
MflI RGATCY 2 cut(s) 706, 1522
MhlI GDGCHC 5 cut(s) 513, 621, 1210, 1618, 2491
Mly113I GGCGCC 1 cut(s) 1868
MlyI GAGTC 1 cut(s) 1915
MmeI TCCRAC 1 cut(s) 1515
Mph1103I ATGCAT 1 cut(s) 1080
MroXI GAANNNNTTC 2 cut(s) 1967, 2350
MslI CAYNNNNRTG 2 cut(s) 275, 1474
MspA1I CMGCKG 3 cut(s) 24, 827, 2399
MspI CCGG 5 cut(s) 679, 1280, 1328, 1871, 2823
MunI CAATTG 1 cut(s) 2235
MvaI CCWGG 8 cut(s) 79, 489, 606, 777, 2229, 2659, 2682, 2912
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 7 cut(s) 326, 374, 383, 1505, 1837, 2728, 2750
NarI GGCGCC 1 cut(s) 1868
NciI CCSGG 2 cut(s) 679, 1329
NdeI CATATG 2 cut(s) 1032, 2839
NlaIV GGNNCC 7 cut(s) 41, 87, 1284, 1326, 1591, 1869, 2191
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 2513
NsiI ATGCAT 1 cut(s) 1080
OliI CACNNNNGTG 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 2839
PasI CCCWGGG 1 cut(s) 78
PciSI GCTCTTC 3 cut(s) 1266, 1623, 2751
PcsI WCGNNNNNNNCGW 1 cut(s) 352
PdmI GAANNNNTTC 2 cut(s) 1967, 2350
PfeI GAWTC 4 cut(s) 337, 470, 1968, 2780
PflMI CCANNNNNTGG 2 cut(s) 2315, 2444
PkrI GCNGC 8 cut(s) 367, 379, 826, 856, 2083, 2263, 2337, 2847
Ple19I CGATCG 2 cut(s) 54, 644
PleI GAGTC 1 cut(s) 1914
PluTI GGCGCC 1 cut(s) 1871
PpsI GAGTC 1 cut(s) 1914
PpuMI RGGWCCY 1 cut(s) 85
PshAI GACNNNNGTC 1 cut(s) 2187
PshBI ATTAAT 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 2491
Psp1406I AACGTT 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 8 cut(s) 77, 487, 604, 775, 2227, 2657, 2680, 2910
PspFI CCCAGC 1 cut(s) 2399
PspGI CCWGG 8 cut(s) 77, 487, 604, 775, 2227, 2657, 2680, 2910
PspN4I GGNNCC 7 cut(s) 41, 87, 1284, 1326, 1591, 1869, 2191
PspPI GGNCC 5 cut(s) 85, 2189, 2402, 2481, 2834
PspPPI RGGWCCY 1 cut(s) 85
PstI CTGCAG 1 cut(s) 2083
PstNI CAGNNNCTG 1 cut(s) 2105
PsuI RGATCY 2 cut(s) 706, 1522
PvuI CGATCG 2 cut(s) 54, 644
PvuII CAGCTG 2 cut(s) 827, 2399
RsaI GTAC 3 cut(s) 41, 1886, 2603
RsaNI GTAC 3 cut(s) 40, 1885, 2602
RseI CAYNNNNRTG 2 cut(s) 275, 1474
SacI GAGCTC 1 cut(s) 2491
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 3 cut(s) 1266, 1623, 2751
SatI GCNGC 8 cut(s) 366, 378, 825, 855, 2082, 2262, 2336, 2846
Sau96I GGNCC 5 cut(s) 85, 2189, 2402, 2481, 2834
SchI GAGTC 1 cut(s) 1915
SduI GDGCHC 5 cut(s) 513, 621, 1210, 1618, 2491
SexAI ACCWGGT 1 cut(s) 2657
SfaNI GCATC 9 cut(s) 364, 583, 691, 1065, 1114, 1818, 2478, 2676, 2821
SfcI CTRYAG 2 cut(s) 2079, 2667
SfoI GGCGCC 1 cut(s) 1869
Sfr274I CTCGAG 1 cut(s) 118
SgrDI CGTCGACG 1 cut(s) 56
SinI GGWCC 3 cut(s) 85, 2189, 2834
SlaI CTCGAG 1 cut(s) 118
SmiI ATTTAAAT 1 cut(s) 544
SmiMI CAYNNNNRTG 2 cut(s) 275, 1474
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SspDI GGCGCC 1 cut(s) 1867
SspI AATATT 4 cut(s) 246, 530, 753, 920
SspMI CTAG 4 cut(s) 1526, 2301, 2478, 2628
SstI GAGCTC 1 cut(s) 2491
StyI CCWWGG 2 cut(s) 1456, 2109
SwaI ATTTAAAT 1 cut(s) 544
TaaI ACNGT 4 cut(s) 560, 2248, 2550, 2601
TaiI ACGT 7 cut(s) 63, 117, 234, 358, 603, 2007, 2881
TatI WGTACW 1 cut(s) 1884
TauI GCSGC 2 cut(s) 380, 857
TfiI GAWTC 4 cut(s) 337, 470, 1968, 2780
TscAI CASTG 1 cut(s) 1293
TseFI GTSAC 1 cut(s) 2513
TseI GCWGC 6 cut(s) 365, 824, 2081, 2261, 2335, 2845
Tsp45I GTSAC 1 cut(s) 2513
TspGWI ACGGA 4 cut(s) 1136, 1641, 1814, 2417
TspRI CASTG 1 cut(s) 1293
Van91I CCANNNNNTGG 2 cut(s) 2315, 2444
VpaK11BI GGWCC 3 cut(s) 85, 2189, 2834
VspI ATTAAT 1 cut(s) 1065
XapI RAATTY 7 cut(s) 416, 1094, 1191, 1212, 1636, 2449, 2692
XbaI TCTAGA 1 cut(s) 1525
XcmI CCANNNNNNNNNTGG 1 cut(s) 2763
XhoI CTCGAG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 57, 572
XmnI GAANNNNTTC 2 cut(s) 1967, 2350
XspI CTAG 4 cut(s) 1526, 2301, 2478, 2628
ZraI GACGTC 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.