MD04G1186700.v1.1

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005987
Physical Location & Seq
Forward (+)
27757193 .. 27759283
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1186700.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGCCTCCTCGCCGGACCACCACATCAACGGCGACACCAAAACCCACATCTCCAAAAAACCTAAACTTTCCCCAAATTTCATCACTGCCGCCGAAATCGCCGCCGAATTCTCCCACCACGACCCGAACGTCGCTCGGATCAACAACGGCAGCTTCGGCTCCTGCCCCGCCTCCTTGATCGAAGCCCAGCGGCGGTGGCAGCTCAAAAACCTCGCCCAGCCCGACCATTTCTACGTCAACGAGCTCAAGAAGGGAATCCACCGTTCCAGAACCATTATCAAGGAGCTCATCAATGCCGACCATGTCGACGAGGTCTCACTCGTCGACAACGCCACCACCGCCGCCGCCATCGTGCTTCAGCAGACGGCCTGGGGGTTTTCGGAGAGCAAATTCGGCAAAGGTGATGCTGTGGTGATGCTTCATTACGCTTATGGCGCCGTGAAGAAGTCGATTGAGGCGTACGTGACGCGTGCCGGCGGGTATGTGATTGAAGTCCCTTTGCCTTTTCCTTTGAAATCAAATGATGAAATTATCTCTGAATTTAAGAAAGCTTTGGTGATAGGGAAGGCTAATGGTAGGAGGGTTAGGTTAGCTGTGATTGATCATATTACTTCAATGCCCTGTGTTGTTATACCTGTTGAAGAATTAGTTAAGATTTGTAGGGAAGAGGATGTTGATCAGATTTTTGTAGATGCTGCTCATGGTATCGGGTGTACTGATGTCGATATGAAGCGAATTGGGGCGGATTATTATACTAGTAATTTGCATAAGTGGTTCTTTTGCCCCCCGTCTATTGCGTTTTTGTACTGTAGGAAGTCTCCCAAGTGCCCAGAGTTGCACCATCCTGTTGTTTCCCATGAGTATGGTAATGGGTTGGCTATAGAAAGTGCTTCGGTTGGGAATAGGGATTATAGCCCGCAATTGGTAGTGCCTTCAGTTTTGGAATTCATCAATAGGTTCGAAGGAGGTATTGAGGGGATCAAGAAGAGGAATCATGAGACTGTTGTTGAGATGGGGCAGATGTTGGCCAATGCATGGGGAACGCATCTCGGGTGCCCTTCAGAGATGTGCGCCAGTATGATCATGATTGGGTTGCCTGCTTGCTTGGGGATCTTGAGTGAGAAGGATACTGAGGAGTTAAGGATACATTTGAGGGAGAAGTTTGGTGTTGAAGTTCCGATTTATTTCCGACCACCCAAGAATGGGGAGGTTGAGTCAATAAATGGGTATTGTCGAATTTCTCATCAAGTGTACAACAAAGTTGATGACTATTACAAGTTCAGAGACGCAATTAATCAACTTGTTTCTGAGGGGTTTACTTGTGCTCAACTTTCTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

447

Amino Acids

49.64

Weight (kDa)

6.44

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 75 - 273 1.3e-14 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AccB1I GGYRCC 2 cut(s) 434, 1053
AccB7I CCANNNNNTGG 1 cut(s) 1035
AccI GTMKAC 2 cut(s) 306, 324
AccII CGCG 1 cut(s) 469
AclWI GGATC 3 cut(s) 146, 986, 1118
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 6 cut(s) 76, 107, 389, 539, 944, 1236
AcuI CTGAAG 3 cut(s) 341, 918, 1044
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 4 cut(s) 461, 715, 806, 1253
AfiI CCNNNNNNNGG 5 cut(s) 192, 922, 1035, 1202, 1203
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 5 cut(s) 491, 514, 615, 641, 1172
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 368
AluBI AGCT 6 cut(s) 153, 202, 244, 286, 551, 593
AluI AGCT 6 cut(s) 153, 202, 244, 286, 551, 593
Alw21I GWGCWC 3 cut(s) 246, 288, 1327
Alw26I GTCTC 4 cut(s) 319, 822, 992, 1278
AlwI GGATC 3 cut(s) 146, 986, 1118
Ama87I CYCGRG 1 cut(s) 1049
AoxI GGCC 3 cut(s) 3, 366, 1026
ApeKI GCWGC 3 cut(s) 150, 199, 695
ApoI RAATTY 6 cut(s) 76, 107, 389, 539, 944, 1236
AseI ATTAAT 1 cut(s) 1293
AspLEI GCGC 2 cut(s) 437, 1073
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 413, 424, 568
AsuII TTCGAA 1 cut(s) 960
AvaI CYCGRG 1 cut(s) 1049
AvaII GGWCC 1 cut(s) 16
BaeGI GKGCMC 2 cut(s) 830, 1058
BalI TGGCCA 1 cut(s) 1028
BanI GGYRCC 2 cut(s) 434, 1053
BanII GRGCYC 2 cut(s) 246, 288
Bbv12I GWGCWC 3 cut(s) 246, 288, 1327
BbvI GCAGC 3 cut(s) 162, 211, 682
BccI CCATC 3 cut(s) 356, 849, 1006
BceAI ACGGC 4 cut(s) 46, 163, 381, 422
BciT130I CCWGG 1 cut(s) 370
BciVI GTATCC 2 cut(s) 1120, 1137
BclI TGATCA 3 cut(s) 601, 676, 1080
BcoDI GTCTC 4 cut(s) 319, 822, 992, 1278
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 2 cut(s) 808, 879
BfoI RGCGCY 1 cut(s) 438
BfuI GTATCC 2 cut(s) 1120, 1137
BisI GCNGC 8 cut(s) 90, 102, 151, 191, 200, 342, 345, 696
BlsI GCNGC 8 cut(s) 91, 103, 152, 192, 201, 343, 346, 697
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 3 cut(s) 160, 436, 1055
BmrFI CCNGG 1 cut(s) 370
BmsI GCATC 4 cut(s) 394, 405, 682, 1054
Bpu14I TTCGAA 1 cut(s) 960
BpuEI CTTGAG 2 cut(s) 230, 1135
BsaAI YACGTR 1 cut(s) 463
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 1199, 1229
Bsc4I CCNNNNNNNGG 5 cut(s) 192, 922, 1035, 1202, 1203
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 1074
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 676, 841
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 5 cut(s) 192, 922, 1035, 1202, 1203
BseMII CTCAG 2 cut(s) 1122, 1299
BseNI ACTGG 1 cut(s) 1074
BseRI GAGGAG 1 cut(s) 1148
BseSI GKGCMC 2 cut(s) 830, 1058
BseXI GCAGC 3 cut(s) 162, 211, 682
BseYI CCCAGC 2 cut(s) 186, 216
Bsh1236I CGCG 1 cut(s) 469
BshFI GGCC 3 cut(s) 5, 368, 1028
BshNI GGYRCC 2 cut(s) 434, 1053
BsiHKAI GWGCWC 3 cut(s) 246, 288, 1327
BsiHKCI CYCGRG 1 cut(s) 1049
BsiSI CCGG 2 cut(s) 14, 474
BsiWI CGTACG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 5 cut(s) 192, 922, 1035, 1202, 1203
BsmAI GTCTC 4 cut(s) 319, 822, 992, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 3 cut(s) 5, 368, 1028
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 1049
Bsp119I TTCGAA 1 cut(s) 960
Bsp1286I GDGCHC 5 cut(s) 246, 288, 830, 1058, 1327
Bsp1407I TGTACA 1 cut(s) 1251
Bsp143I GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
BspANI GGCC 3 cut(s) 5, 368, 1028
BspCNI CTCAG 2 cut(s) 1123, 1300
BspFNI CGCG 1 cut(s) 469
BspHI TCATGA 2 cut(s) 994, 1083
BspLI GGNNCC 3 cut(s) 160, 436, 1055
BspPI GGATC 3 cut(s) 146, 986, 1118
BspT104I TTCGAA 1 cut(s) 960
BspT107I GGYRCC 2 cut(s) 434, 1053
BspTNI GGTCTC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 1251
BsrI ACTGG 1 cut(s) 1074
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
BssNI GRCGYC 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 370
Bst4CI ACNGT 3 cut(s) 263, 809, 1003
Bst6I CTCTTC 2 cut(s) 660, 980
BstACI GRCGYC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 1251
BstBAI YACGTR 1 cut(s) 463
BstBI TTCGAA 1 cut(s) 960
BstC8I GCNNGC 5 cut(s) 471, 475, 917, 1098, 1102
BstDEI CTNAG 2 cut(s) 1131, 1308
BstF5I GGATG 2 cut(s) 676, 841
BstFNI CGCG 1 cut(s) 469
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 2 cut(s) 437, 1073
BstKTI GATC 7 cut(s) 141, 180, 604, 679, 981, 1083, 1113
BstMAI GTCTC 4 cut(s) 319, 822, 992, 1278
BstMBI GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
BstMWI GCNNNNNNNGC 7 cut(s) 98, 156, 196, 199, 338, 393, 434
BstNI CCWGG 1 cut(s) 370
BstSCI CCNGG 1 cut(s) 368
BstSFI CTRYAG 2 cut(s) 808, 879
BstSLI GKGCMC 2 cut(s) 830, 1058
BstUI CGCG 1 cut(s) 469
BstV1I GCAGC 3 cut(s) 162, 211, 682
BstX2I RGATCY 1 cut(s) 1110
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 1 cut(s) 1110
BsuI GTATCC 2 cut(s) 1120, 1137
BsuRI GGCC 3 cut(s) 5, 368, 1028
BtsCI GGATG 2 cut(s) 676, 841
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 5 cut(s) 471, 475, 917, 1098, 1102
CciI TCATGA 2 cut(s) 994, 1083
CfoI GCGC 2 cut(s) 437, 1073
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 2 cut(s) 475, 1295
Csp6I GTAC 4 cut(s) 460, 714, 805, 1252
CviAII CATG 6 cut(s) 302, 701, 857, 995, 1035, 1084
CviQI GTAC 4 cut(s) 460, 714, 805, 1252
DdeI CTNAG 2 cut(s) 1131, 1308
DinI GGCGCC 1 cut(s) 436
DpnI GATC 7 cut(s) 140, 179, 603, 678, 980, 1082, 1112
DpnII GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 2 cut(s) 660, 980
EarI CTCTTC 2 cut(s) 660, 980
EciI GGCGGA 1 cut(s) 758
Ecl136II GAGCTC 2 cut(s) 244, 286
Eco24I GRGCYC 2 cut(s) 246, 288
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 2 cut(s) 244, 286
Eco57I CTGAAG 3 cut(s) 341, 918, 1044
Eco88I CYCGRG 1 cut(s) 1049
EcoICRI GAGCTC 2 cut(s) 244, 286
EcoRI GAATTC 2 cut(s) 107, 944
EcoRII CCWGG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 1036
EcoT38I GRGCYC 2 cut(s) 246, 288
EgeI GGCGCC 1 cut(s) 436
EheI GGCGCC 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 1278
FaeI CATG 6 cut(s) 305, 704, 860, 998, 1038, 1087
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 479
FatI CATG 6 cut(s) 301, 700, 856, 994, 1034, 1083
FauI CCCGC 3 cut(s) 175, 470, 924
FbaI TGATCA 3 cut(s) 601, 676, 1080
FblI GTMKAC 2 cut(s) 306, 324
Fnu4HI GCNGC 8 cut(s) 90, 102, 151, 191, 200, 342, 345, 696
FokI GGATG 2 cut(s) 683, 828
FriOI GRGCYC 2 cut(s) 246, 288
Fsp4HI GCNGC 8 cut(s) 90, 102, 151, 191, 200, 342, 345, 696
FspBI CTAG 1 cut(s) 756
GlaI GCGC 2 cut(s) 436, 1072
GluI GCNGC 8 cut(s) 90, 102, 151, 191, 200, 342, 345, 696
GsaI CCCAGC 2 cut(s) 190, 220
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 3 cut(s) 5, 368, 1028
HapII CCGG 2 cut(s) 14, 474
HgaI GACGC 2 cut(s) 475, 1295
HhaI GCGC 2 cut(s) 437, 1073
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 6 cut(s) 305, 704, 860, 998, 1038, 1087
Hin6I GCGC 2 cut(s) 435, 1071
HinP1I GCGC 2 cut(s) 435, 1071
HincII GTYRAC 3 cut(s) 238, 307, 325
HindII GTYRAC 3 cut(s) 238, 307, 325
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 3 cut(s) 255, 991, 1214
HpaII CCGG 2 cut(s) 14, 474
HphI GGTGA 3 cut(s) 413, 424, 568
Hpy166II GTNNAC 6 cut(s) 238, 307, 325, 714, 1252, 1317
Hpy188I TCNGA 9 cut(s) 138, 382, 538, 681, 1063, 1179, 1190, 1283, 1309
Hpy188III TCNNGA 6 cut(s) 247, 267, 982, 995, 1084, 1114
Hpy8I GTNNAC 6 cut(s) 238, 307, 325, 714, 1252, 1317
Hpy99I CGWCG 3 cut(s) 134, 311, 326
HpyAV CCTTC 6 cut(s) 244, 559, 942, 956, 1068, 1117
HpyCH4III ACNGT 3 cut(s) 263, 809, 1003
HpyCH4IV ACGT 3 cut(s) 129, 234, 462
HpyCH4V TGCA 3 cut(s) 766, 838, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 156, 196, 199, 338, 393, 434
HpyF3I CTNAG 2 cut(s) 1131, 1308
HpySE526I ACGT 3 cut(s) 129, 234, 462
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 6 cut(s) 305, 704, 860, 998, 1038, 1087
HspAI GCGC 2 cut(s) 435, 1071
KasI GGCGCC 1 cut(s) 434
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Ksp22I TGATCA 3 cut(s) 601, 676, 1080
Kzo9I GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
LmnI GCTCC 2 cut(s) 164, 283
Lsp1109I GCAGC 3 cut(s) 162, 211, 682
LweI GCATC 4 cut(s) 394, 405, 682, 1054
MaeI CTAG 1 cut(s) 756
MaeII ACGT 3 cut(s) 129, 234, 462
MaeIII GTNAC 1 cut(s) 463
MalI GATC 7 cut(s) 140, 179, 603, 678, 980, 1082, 1112
MboI GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
MboII GAAGA 4 cut(s) 454, 653, 677, 997
MfeI CAATTG 1 cut(s) 920
MflI RGATCY 1 cut(s) 1110
MhlI GDGCHC 5 cut(s) 246, 288, 830, 1058, 1327
MlsI TGGCCA 1 cut(s) 1028
MluI ACGCGT 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 1028
Mly113I GGCGCC 1 cut(s) 435
MlyI GAGTC 1 cut(s) 1223
MmeI TCCRAC 1 cut(s) 1213
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 1 cut(s) 1036
MroNI GCCGGC 1 cut(s) 473
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 4 cut(s) 543, 651, 1139, 1293
MslI CAYNNNNRTG 1 cut(s) 861
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 14, 474
MspR9I CCNGG 1 cut(s) 370
MunI CAATTG 1 cut(s) 920
MvaI CCWGG 1 cut(s) 370
MvnI CGCG 1 cut(s) 469
MwoI GCNNNNNNNGC 7 cut(s) 98, 156, 196, 199, 338, 393, 434
NaeI GCCGGC 1 cut(s) 475
NarI GGCGCC 1 cut(s) 435
NdeII GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 6 cut(s) 305, 704, 860, 998, 1038, 1087
NlaIV GGNNCC 3 cut(s) 160, 436, 1055
NmuCI GTSAC 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 1036
NspV TTCGAA 1 cut(s) 960
PagI TCATGA 2 cut(s) 994, 1083
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 219, 455
PdiI GCCGGC 1 cut(s) 475
PfeI GAWTC 2 cut(s) 255, 991
Pfl23II CGTACG 1 cut(s) 459
PflFI GACNNNGTC 2 cut(s) 302, 311
PflMI CCANNNNNTGG 1 cut(s) 1035
PkrI GCNGC 8 cut(s) 91, 103, 152, 192, 201, 343, 346, 697
PleI GAGTC 1 cut(s) 1222
PluTI GGCGCC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 1222
Ppu21I YACGTR 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 1293
Psp124BI GAGCTC 2 cut(s) 246, 288
Psp6I CCWGG 1 cut(s) 368
PspFI CCCAGC 2 cut(s) 186, 216
PspGI CCWGG 1 cut(s) 368
PspLI CGTACG 1 cut(s) 459
PspN4I GGNNCC 3 cut(s) 160, 436, 1055
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 1 cut(s) 1110
PsyI GACNNNGTC 2 cut(s) 302, 311
RsaI GTAC 4 cut(s) 461, 715, 806, 1253
RsaNI GTAC 4 cut(s) 460, 714, 805, 1252
RseI CAYNNNNRTG 1 cut(s) 861
SacI GAGCTC 2 cut(s) 246, 288
SalI GTCGAC 2 cut(s) 305, 323
SaqAI TTAA 4 cut(s) 543, 651, 1139, 1293
SatI GCNGC 8 cut(s) 90, 102, 151, 191, 200, 342, 345, 696
Sau3AI GATC 7 cut(s) 138, 177, 601, 676, 978, 1080, 1110
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 1 cut(s) 1223
ScrFI CCNGG 1 cut(s) 370
SduI GDGCHC 5 cut(s) 246, 288, 830, 1058, 1327
SfaNI GCATC 4 cut(s) 394, 405, 682, 1054
SfcI CTRYAG 2 cut(s) 808, 879
SfoI GGCGCC 1 cut(s) 436
SfuI TTCGAA 1 cut(s) 960
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 2 cut(s) 245, 1114
SmoI CTYRAG 2 cut(s) 245, 1114
SpeI ACTAGT 1 cut(s) 755
SspDI GGCGCC 1 cut(s) 434
SspMI CTAG 1 cut(s) 756
SstI GAGCTC 2 cut(s) 246, 288
StyD4I CCNGG 1 cut(s) 368
TaaI ACNGT 3 cut(s) 263, 809, 1003
TaiI ACGT 3 cut(s) 132, 237, 465
TaqI TCGA 7 cut(s) 180, 306, 324, 449, 723, 960, 1234
TatI WGTACW 3 cut(s) 713, 804, 1251
TauI GCSGC 5 cut(s) 92, 104, 193, 344, 347
TfiI GAWTC 2 cut(s) 255, 991
Tru1I TTAA 4 cut(s) 543, 651, 1139, 1293
Tru9I TTAA 4 cut(s) 543, 651, 1139, 1293
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 3 cut(s) 150, 199, 695
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 5 cut(s) 70, 410, 540, 743, 937
TspRI CASTG 1 cut(s) 91
Tth111I GACNNNGTC 2 cut(s) 302, 311
Van91I CCANNNNNTGG 1 cut(s) 1035
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 1293
XapI RAATTY 6 cut(s) 76, 107, 389, 539, 944, 1236
XmiI GTMKAC 2 cut(s) 306, 324
XspI CTAG 1 cut(s) 756
Zsp2I ATGCAT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.