RchiOBHm_Chr5g0036671

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005987
Physical Location & Seq
Forward (+)
30859190 .. 30860128
939 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31548

Sequence Viewer

Length: 939 bp
ATGGCCTCCTCCCCTGACCACAACCACACCTTGAACGGCAATTCCAACACCCACGCCTCCAAAAAGCCCAAACTCTCCTTCATCACCCCCTCCGAATTCTCCCACCACGACCCCACCGTCGCTCGAATCAACAACGGCAGCTTCGGGTCCTGCCCCGACTCCGTCCTCCAGGCCCAGCGCCGCTGGCAGCTCAAGTACCTCTCCCAGCCCGATCACTTCTACTTCAACCACCTCCAAGCCGGCATCCTCCACTCCCGCACCCTCATCAAGGACCTCATCAACGCCGACCACGTCGACGAGGTCTCCATCATCGACAACGCCACCACCGCCGTCGCCATCGTCCTCCAGCAAGCCGCCTGGGGCTTCTCCGAGAGCAAGTTCAAGCAGGGCGACGCCGTCATCATGCTCCACTACGCCTACGGCGCCGTCAAGAAGTCGATTGAGGCCTACATCACTCGCGCCGGCGGGCACGTCATTGAGGTACCCTTGCCATTTCCTTTGAATTCCAACGATGAGATTGTAACCGAGTTTAAGAAGGCTTTGGAGAAGGGGAAGGCTAATGGTAGGAGGGTTAGGTTAGCTGTGATTGATCATATTACTTCAATGCCGTGTGTGGTTATACCTGTTAAGGAGTTGGTTAAGATTTGTAGGGAGGAGGGAGTTGATCAGGTTTTAGTCGATGCGGCACACAGCATTGGGTGCACTAAGGTTGATATGAAGGAGATTGGGGCTGATTATTATACTAGTAATTTGCACAAGTGGTTCTTTTGCCCGCCGTCGATTGCGTTTTTGTATTGTAGGAAGTCGCCCAAGGGTGTGGAATTGCACCACCCGGTGGTTTCCCATGAGTATGGGAATGGATTGGCTATTGAAAGTGCTTGGATTGGGACTAGGGATTACAGCCCTCAGTTGGTTGTAAGTGTCATTTCTATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

312

Amino Acids

34.59

Weight (kDa)

7.29

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 52 - 270 2.4e-17 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
Acc65I GGTACC 1 cut(s) 481
AccB1I GGYRCC 2 cut(s) 422, 481
AccB7I CCANNNNNTGG 1 cut(s) 835
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 459
AciI CCGC 7 cut(s) 181, 256, 327, 354, 465, 683, 773
AcsI RAATTY 2 cut(s) 95, 502
AcyI GRCGYC 2 cut(s) 393, 423
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 2 cut(s) 197, 483
AfiI CCNNNNNNNGG 3 cut(s) 268, 835, 910
AgsI TTSAA 6 cut(s) 34, 226, 382, 502, 603, 872
AhlI ACTAGT 1 cut(s) 743
AjiI CACGTC 2 cut(s) 292, 472
AjnI CCWGG 2 cut(s) 168, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
AluBI AGCT 3 cut(s) 141, 190, 581
AluI AGCT 3 cut(s) 141, 190, 581
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 1 cut(s) 307
Alw44I GTGCAC 1 cut(s) 700
AoxI GGCC 3 cut(s) 3, 171, 444
ApaLI GTGCAC 1 cut(s) 700
ApeKI GCWGC 2 cut(s) 138, 187
ApoI RAATTY 2 cut(s) 95, 502
Asp718I GGTACC 1 cut(s) 481
AspLEI GCGC 3 cut(s) 180, 425, 461
AspS9I GGNCC 3 cut(s) 147, 172, 271
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 2 cut(s) 147, 271
BaeGI GKGCMC 2 cut(s) 471, 704
BanI GGYRCC 2 cut(s) 422, 481
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 2 cut(s) 150, 199
BccI CCATC 2 cut(s) 314, 344
BceAI ACGGC 8 cut(s) 52, 151, 314, 380, 410, 436, 592, 760
BciT130I CCWGG 2 cut(s) 170, 358
BclI TGATCA 2 cut(s) 589, 664
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 1 cut(s) 307
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 2 cut(s) 744, 891
BfoI RGCGCY 2 cut(s) 181, 426
BisI GCNGC 5 cut(s) 139, 181, 188, 354, 684
BlsI GCNGC 5 cut(s) 140, 182, 189, 355, 685
Bme1390I CCNGG 3 cut(s) 170, 358, 833
Bme18I GGWCC 2 cut(s) 147, 271
BmgBI CACGTC 2 cut(s) 292, 472
BmgT120I GGNCC 3 cut(s) 147, 172, 271
BmiI GGNNCC 3 cut(s) 148, 424, 483
BmrFI CCNGG 3 cut(s) 170, 358, 833
BmsI GCATC 2 cut(s) 252, 670
BpmI CTGGAG 2 cut(s) 152, 329
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 833
BsaHI GRCGYC 2 cut(s) 393, 423
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 357, 810
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 835, 910
Bse118I RCCGGY 2 cut(s) 239, 461
BseBI CCWGG 2 cut(s) 170, 358
BseDI CCNNGG 2 cut(s) 357, 810
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 268, 835, 910
BseMII CTCAG 1 cut(s) 920
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 2 cut(s) 471, 704
BseXI GCAGC 2 cut(s) 150, 199
BseYI CCCAGC 2 cut(s) 174, 204
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 3 cut(s) 5, 173, 446
BshNI GGYRCC 2 cut(s) 422, 481
BsiHKAI GWGCWC 1 cut(s) 704
BsiSI CCGG 3 cut(s) 240, 462, 833
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 268, 835, 910
BsmAI GTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 901
BsnI GGCC 3 cut(s) 5, 173, 446
Bso31I GGTCTC 1 cut(s) 307
Bsp1286I GDGCHC 2 cut(s) 471, 704
Bsp143I GATC 3 cut(s) 211, 589, 664
BspACI CCGC 7 cut(s) 181, 256, 327, 354, 465, 683, 773
BspANI GGCC 3 cut(s) 5, 173, 446
BspCNI CTCAG 1 cut(s) 919
BspFNI CGCG 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 148, 424, 483
BspT107I GGYRCC 2 cut(s) 422, 481
BspTNI GGTCTC 1 cut(s) 307
BsrFI RCCGGY 2 cut(s) 239, 461
BssAI RCCGGY 2 cut(s) 239, 461
BssECI CCNNGG 2 cut(s) 357, 810
BssMI GATC 3 cut(s) 211, 589, 664
BssNI GRCGYC 2 cut(s) 393, 423
BssT1I CCWWGG 1 cut(s) 810
Bst2UI CCWGG 2 cut(s) 170, 358
Bst4CI ACNGT 1 cut(s) 118
BstACI GRCGYC 2 cut(s) 393, 423
BstAPI GCANNNNNTGC 1 cut(s) 699
BstC8I GCNNGC 6 cut(s) 185, 241, 351, 463, 467, 773
BstDEI CTNAG 2 cut(s) 705, 906
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 243
BstFNI CGCG 1 cut(s) 459
BstH2I RGCGCY 2 cut(s) 181, 426
BstHHI GCGC 3 cut(s) 180, 425, 461
BstKTI GATC 3 cut(s) 214, 592, 667
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 3 cut(s) 211, 589, 664
BstMWI GCNNNNNNNGC 4 cut(s) 184, 326, 422, 699
BstNI CCWGG 2 cut(s) 170, 358
BstSCI CCNGG 3 cut(s) 168, 356, 831
BstSLI GKGCMC 2 cut(s) 471, 704
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 2 cut(s) 150, 199
BstXI CCANNNNNNTGG 2 cut(s) 817, 851
BsuRI GGCC 3 cut(s) 5, 173, 446
BtrI CACGTC 2 cut(s) 292, 472
BtsCI GGATG 1 cut(s) 243
Cac8I GCNNGC 6 cut(s) 185, 241, 351, 463, 467, 773
CfoI GCGC 3 cut(s) 180, 425, 461
Cfr10I RCCGGY 2 cut(s) 239, 461
Cfr13I GGNCC 3 cut(s) 147, 172, 271
CseI GACGC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 196, 482
CviAII CATG 2 cut(s) 403, 845
CviQI GTAC 2 cut(s) 196, 482
DdeI CTNAG 2 cut(s) 705, 906
DinI GGCGCC 1 cut(s) 424
DpnI GATC 3 cut(s) 213, 591, 666
DpnII GATC 3 cut(s) 211, 589, 664
DraIII CACNNNGTG 1 cut(s) 835
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 810
Eco147I AGGCCT 1 cut(s) 446
Eco31I GGTCTC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 147, 271
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoO109I RGGNCCY 2 cut(s) 147, 271
EcoRI GAATTC 2 cut(s) 95, 502
EcoRII CCWGG 2 cut(s) 168, 356
EcoT14I CCWWGG 1 cut(s) 810
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 2 cut(s) 406, 848
FaiI YATR 8 cut(s) 404, 594, 620, 716, 741, 846, 852, 932
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 1 cut(s) 901
FatI CATG 2 cut(s) 402, 844
FauI CCCGC 3 cut(s) 263, 458, 780
FbaI TGATCA 2 cut(s) 589, 664
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 139, 181, 188, 354, 684
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 139, 181, 188, 354, 684
FspBI CTAG 2 cut(s) 744, 891
GlaI GCGC 3 cut(s) 179, 424, 460
GluI GCNGC 5 cut(s) 139, 181, 188, 354, 684
GsaI CCCAGC 2 cut(s) 178, 208
GsuI CTGGAG 2 cut(s) 152, 329
HaeII RGCGCY 2 cut(s) 181, 426
HaeIII GGCC 3 cut(s) 5, 173, 446
HapII CCGG 3 cut(s) 240, 462, 833
HgaI GACGC 1 cut(s) 401
HhaI GCGC 3 cut(s) 180, 425, 461
Hin1I GRCGYC 2 cut(s) 393, 423
Hin1II CATG 2 cut(s) 406, 848
Hin6I GCGC 3 cut(s) 178, 423, 459
HinP1I GCGC 3 cut(s) 178, 423, 459
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 126, 158
HpaII CCGG 3 cut(s) 240, 462, 833
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 2 cut(s) 295, 702
Hpy188I TCNGA 2 cut(s) 94, 370
Hpy188III TCNNGA 1 cut(s) 430
Hpy8I GTNNAC 2 cut(s) 295, 702
Hpy99I CGWCG 6 cut(s) 122, 296, 299, 335, 395, 781
HpyAV CCTTC 5 cut(s) 88, 529, 541, 547, 712
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 2 cut(s) 291, 471
HpyCH4V TGCA 3 cut(s) 702, 754, 826
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 326, 422, 699
HpyF3I CTNAG 2 cut(s) 705, 906
HpySE526I ACGT 2 cut(s) 291, 471
Hsp92I GRCGYC 2 cut(s) 393, 423
Hsp92II CATG 2 cut(s) 406, 848
HspAI GCGC 3 cut(s) 178, 423, 459
KasI GGCGCC 1 cut(s) 422
KpnI GGTACC 1 cut(s) 485
KroI GCCGGC 2 cut(s) 239, 461
KroNI GCCGGC 2 cut(s) 241, 463
Ksp22I TGATCA 2 cut(s) 589, 664
Kzo9I GATC 3 cut(s) 211, 589, 664
LmnI GCTCC 1 cut(s) 411
Lsp1109I GCAGC 2 cut(s) 150, 199
LweI GCATC 2 cut(s) 252, 670
MaeI CTAG 2 cut(s) 744, 891
MaeII ACGT 2 cut(s) 291, 471
MaeIII GTNAC 1 cut(s) 520
MalI GATC 3 cut(s) 213, 591, 666
MboI GATC 3 cut(s) 211, 589, 664
MhlI GDGCHC 2 cut(s) 471, 704
MluCI AATT 5 cut(s) 40, 95, 502, 748, 821
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 69, 531
MreI CGCCGGCG 1 cut(s) 461
MroNI GCCGGC 2 cut(s) 239, 461
MseI TTAA 3 cut(s) 531, 627, 639
MslI CAYNNNNRTG 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 3 cut(s) 240, 462, 833
MspR9I CCNGG 3 cut(s) 170, 358, 833
MvaI CCWGG 2 cut(s) 170, 358
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 184, 326, 422, 699
NaeI GCCGGC 2 cut(s) 241, 463
NarI GGCGCC 1 cut(s) 423
NciI CCSGG 1 cut(s) 833
NdeII GATC 3 cut(s) 211, 589, 664
NgoMIV GCCGGC 2 cut(s) 239, 461
NlaIII CATG 2 cut(s) 406, 848
NlaIV GGNNCC 3 cut(s) 148, 424, 483
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 288
PdiI GCCGGC 2 cut(s) 241, 463
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 4 cut(s) 161, 290, 299, 395
PflMI CCANNNNNTGG 1 cut(s) 835
PkrI GCNGC 5 cut(s) 140, 182, 189, 355, 685
PleI GAGTC 1 cut(s) 152
PluTI GGCGCC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 152
PpuMI RGGWCCY 2 cut(s) 147, 271
Psp5II RGGWCCY 2 cut(s) 147, 271
Psp6I CCWGG 2 cut(s) 168, 356
PspFI CCCAGC 2 cut(s) 174, 204
PspGI CCWGG 2 cut(s) 168, 356
PspN4I GGNNCC 3 cut(s) 148, 424, 483
PspPI GGNCC 3 cut(s) 147, 172, 271
PspPPI RGGWCCY 2 cut(s) 147, 271
PsyI GACNNNGTC 4 cut(s) 161, 290, 299, 395
RsaI GTAC 2 cut(s) 197, 483
RsaNI GTAC 2 cut(s) 196, 482
RseI CAYNNNNRTG 1 cut(s) 849
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 531, 627, 639
SatI GCNGC 5 cut(s) 139, 181, 188, 354, 684
Sau3AI GATC 3 cut(s) 211, 589, 664
Sau96I GGNCC 3 cut(s) 147, 172, 271
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 170, 358, 833
SduI GDGCHC 2 cut(s) 471, 704
SfaNI GCATC 2 cut(s) 252, 670
SfoI GGCGCC 1 cut(s) 424
SgrAI CRCCGGYG 1 cut(s) 461
SgrDI CGTCGACG 1 cut(s) 293
SinI GGWCC 2 cut(s) 147, 271
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SpeI ACTAGT 1 cut(s) 743
Sse9I AATT 5 cut(s) 40, 95, 502, 748, 821
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 7 cut(s) 181, 256, 327, 354, 465, 683, 773
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 2 cut(s) 744, 891
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 3 cut(s) 168, 356, 831
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 2 cut(s) 294, 474
TaqI TCGA 6 cut(s) 124, 294, 312, 437, 678, 779
TasI AATT 5 cut(s) 40, 95, 502, 748, 821
TauI GCSGC 3 cut(s) 183, 356, 686
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 3 cut(s) 531, 627, 639
Tru9I TTAA 3 cut(s) 531, 627, 639
TseI GCWGC 2 cut(s) 138, 187
TspDTI ATGAA 2 cut(s) 70, 731
TspGWI ACGGA 1 cut(s) 151
Tth111I GACNNNGTC 4 cut(s) 161, 290, 299, 395
Van91I CCANNNNNTGG 1 cut(s) 835
VneI GTGCAC 1 cut(s) 700
VpaK11BI GGWCC 2 cut(s) 147, 271
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 2 cut(s) 95, 502
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 2 cut(s) 744, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.