Rorug05G0307700

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005987
Physical Location & Seq
Reverse (-)
36019090 .. 36024973
5884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0307700.1

Sequence Viewer

Length: 1959 bp
ATGGCTACTGTGGAAGCTGGTCGGCAACTTTTATATGGGGGGTTTGGTCGCCTCGGCTGTTTTGATCCATGTCTAAGCAATGTAAATGGGGTAATCTCTGTTAAATCTGGTGTAAATAATACACGGAAGAGGAGTTTAGTGCGTGGACAGGTACATAAAAGTTCAAGAGTTCCACGGGATCGCAGGGGGGGTGGTCCCTTTCAGGGTACAGGTGGTGTTTTTCATGGGAAAGGTTGTGCTAGTAGATTGGATTCTATGAGATACAATTGCCAGCGGAAAGAAAGTCTGAACGGAGGAACTGCAGAAGATCAGCGCAGGACGTTGTTTGTGGAGGAATCAGATAAAGTGATATCAGCTCCTTCTAATGGTAAACCCAATTCAGATGTTAATGAATTCAAAGTGGATCAGACACTGGACCAGGGAAATGGGGGTTTTGGGTCGAATGGTAAGCCGGCTGCAGCTGCAATAAATGAAAATTCTTCACAGAAGGTTAAGAGCAATTTAATTGAGGATGAAGCTTGGACTTTACTAAGAAACTCCATGGTCTATTACTGTAGTAATCCTATTGGAACCATTGCTGCTAATGATCCAAGCTCCCCTAGTACTCTGAACTATGATCAGGTCTTTATTCGTGACTTCATACCTTCTGGGATAGCTTTCCTTTTAAAGGGAGAGTATGATATCGTGCGGAACTTCATTCTTCATACCCTTCAGTTGCAGAGCTGGGAGAAAACCATGGATTGTTATAGTCCTGGTCAAGGACTGATGCCTGCCAGTTTTAAGGTTCGCACTGTTCCGCTCGAAGGTGATGACTATGCAACTGAAGATGTGTTAGATCCTGACTTTGGTGAAGCTGCTATTGGTCGTGTTGCCCCAGTTGACTCTGGGTTATGGTGGATTATCTTGTTACGAGCTTATGGAAAATGCTCTGGAGATCTTTCAGTGCAAGAGAGAGTTGATGTGCAAACAGGGATAAAAATGATTTTAAAGCTGTGTCTTGCGGATGGTTTTGATATGTTTCCAACACTACTGGTCACAGATGGTTCTTGCATGATAGATCGTCGGATGGGAATTCATGGTCATCCTTTGGAGATTCAGGCACTGTTCTATTCAGCATTACTTGGTGCACGGGAGATGCTTGCTCCAGAGGATGCATCAGCCGACCTCATCCAAGCGCTTAAAAACCGTCTGGTTGCATTATCCTTTCATATCAGAGAATATTACTGGATTGACATGAGAAAACTGAATGAAATTTACCGATACAAGACAGAGGAATACTCGTATGATGCTGTTAATAAGTTCAATATCTACCCAGATCAGATTCCATCATGGCTCGTGGATTTTATGCCTAGTAAAGGAGGCTATTTGATTGGAAACCTGCAGCCTGCTCACATGGACTTTCGTTTCTTTTCTCTTGGAAACTTGTGGTCTATTGTAAGTAGTCTTGCAACATTGGAACAGTCGCATGCTATATTGGATCTTATTGATGCAAAATGGGAGGAATTGGTGGCAGACATGCCCTTTAAAATATGTTATCCTGCTCTTGAAGGCCGGGAATGGCAAATCATCACAGGCAGTGATCCCAAAAACACTCCCTGGTCTTACCATAATGGAGGTTCTTGGCCAACATTACTATGGCAGCTCACTGTGGCTTGCATAAAGCTCGACAGACCAGAAATTGCATTAAAAGCTCTCAAGCTGGCTGAGAAACGCATATCTCATGACAAGTGGCCGGAATATTATGACACCAAGCGAGCAAGATTTATTGGAAAACAAGCACAGCTGTATCAAACTTGGACGGTTGCTGGATACCTAGTAGCAAAGCTCCTCCTTGATAACCCAAGTGCAGCAAAGAACATTGTGAATGAAGAAGATTCAGAGCTTGTAAATGCATTTTCTTGCATGATAAGTTCCAACCCAAGAAGGAAGCGTGTTTGGAAGAAACAGATTTTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

652

Amino Acids

73.06

Weight (kDa)

6.81

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 173 - 614 4.4e-218 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1386
AccBSI CCGCTC 1 cut(s) 799
AciI CCGC 4 cut(s) 274, 688, 797, 1001
AclWI GGATC 7 cut(s) 59, 186, 411, 581, 830, 1485, 1574
AcoI YGGCCR 2 cut(s) 1622, 1730
AcsI RAATTY 4 cut(s) 392, 475, 1071, 1251
AcuI CTGAAG 2 cut(s) 695, 843
AfaI GTAC 3 cut(s) 153, 208, 604
AfeI AGCGCT 1 cut(s) 1176
AfiI CCNNNNNNNGG 7 cut(s) 203, 365, 667, 758, 803, 845, 1355
AgsI TTSAA 4 cut(s) 165, 397, 1303, 1547
AjnI CCWGG 3 cut(s) 417, 751, 1595
AjuI GAANNNNNNNTTGG 2 cut(s) 843, 875
Alw21I GWGCWC 1 cut(s) 1129
Alw44I GTGCAC 1 cut(s) 1125
AlwI GGATC 7 cut(s) 59, 186, 411, 581, 830, 1485, 1574
AlwNI CAGNNNCTG 2 cut(s) 412, 1102
Aor51HI AGCGCT 1 cut(s) 1176
AoxI GGCC 3 cut(s) 1549, 1622, 1730
ApaLI GTGCAC 1 cut(s) 1125
ApeKI GCWGC 8 cut(s) 455, 458, 461, 578, 854, 1381, 1639, 1847
ApoI RAATTY 4 cut(s) 392, 475, 1071, 1251
AspLEI GCGC 2 cut(s) 315, 1177
AspS9I GGNCC 2 cut(s) 194, 415
AsuC2I CCSGG 1 cut(s) 1553
AsuHPI GGTGA 2 cut(s) 818, 860
AvaII GGWCC 2 cut(s) 194, 415
BaeGI GKGCMC 1 cut(s) 1129
BaeI ACNNNNGTAYC 2 cut(s) 1769, 1802
BalI TGGCCA 1 cut(s) 1624
BauI CACGAG 1 cut(s) 1334
Bbv12I GWGCWC 1 cut(s) 1129
BbvI GCAGC 8 cut(s) 442, 448, 470, 565, 841, 1393, 1651, 1859
BccI CCATC 4 cut(s) 998, 1034, 1060, 1333
BcgI CGANNNNNNTGC 2 cut(s) 1645, 1679
BciT130I CCWGG 3 cut(s) 419, 753, 1597
BciVI GTATCC 1 cut(s) 1802
BclI TGATCA 1 cut(s) 616
BcnI CCSGG 1 cut(s) 1553
BfaI CTAG 4 cut(s) 240, 600, 1350, 1814
BfmI CTRYAG 4 cut(s) 300, 456, 553, 1379
BfoI RGCGCY 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 1386
BfuI GTATCC 1 cut(s) 1802
BglII AGATCT 1 cut(s) 934
BisI GCNGC 8 cut(s) 456, 459, 462, 579, 855, 1382, 1640, 1848
BlsI GCNGC 8 cut(s) 457, 460, 463, 580, 856, 1383, 1641, 1849
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 4 cut(s) 419, 753, 1553, 1597
Bme18I GGWCC 2 cut(s) 194, 415
BmgT120I GGNCC 2 cut(s) 194, 415
BmiI GGNNCC 2 cut(s) 196, 571
BmrFI CCNGG 4 cut(s) 419, 753, 1553, 1597
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 6 cut(s) 756, 1125, 1141, 1163, 1276, 1477
BmuI ACTGGG 1 cut(s) 869
BplI GAGNNNNNCTC 2 cut(s) 1262, 1294
BpmI CTGGAG 2 cut(s) 951, 1128
BpuEI CTTGAG 1 cut(s) 1679
BpuMI CCSGG 1 cut(s) 1553
BsaJI CCNNGG 6 cut(s) 52, 173, 418, 540, 735, 1595
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 365, 667, 758, 803, 845, 1355
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 5 cut(s) 417, 774, 875, 1035, 1229
Bse3DI GCAATG 2 cut(s) 85, 573
BseBI CCWGG 3 cut(s) 419, 753, 1597
BseDI CCNNGG 6 cut(s) 52, 173, 418, 540, 735, 1595
BseGI GGATG 6 cut(s) 517, 1009, 1071, 1081, 1156, 1167
BseLI CCNNNNNNNGG 7 cut(s) 203, 365, 667, 758, 803, 845, 1355
BseMI GCAATG 2 cut(s) 85, 573
BseMII CTCAG 1 cut(s) 1695
BseNI ACTGG 5 cut(s) 417, 774, 875, 1035, 1229
BseRI GAGGAG 2 cut(s) 145, 1817
BseSI GKGCMC 1 cut(s) 1129
BseXI GCAGC 8 cut(s) 442, 448, 470, 565, 841, 1393, 1651, 1859
BseYI CCCAGC 1 cut(s) 723
BsgI GTGCAG 1 cut(s) 1866
BshFI GGCC 3 cut(s) 1551, 1624, 1732
BsiHKAI GWGCWC 1 cut(s) 1129
BsiSI CCGG 3 cut(s) 452, 1552, 1733
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 7 cut(s) 203, 365, 667, 758, 803, 845, 1355
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 3 cut(s) 1551, 1624, 1732
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp19I CCATGG 2 cut(s) 540, 735
BspACI CCGC 4 cut(s) 274, 688, 797, 1001
BspANI GGCC 3 cut(s) 1551, 1624, 1732
BspCNI CTCAG 1 cut(s) 1696
BspHI TCATGA 1 cut(s) 1720
BspLI GGNNCC 2 cut(s) 196, 571
BspMAI CTGCAG 3 cut(s) 304, 460, 1383
BspMI ACCTGC 1 cut(s) 1386
BspPI GGATC 7 cut(s) 59, 186, 411, 581, 830, 1485, 1574
BsrBI CCGCTC 1 cut(s) 799
BsrDI GCAATG 2 cut(s) 85, 573
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 5 cut(s) 417, 774, 875, 1035, 1229
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 6 cut(s) 52, 173, 418, 540, 735, 1595
BssSI CACGAG 1 cut(s) 1334
BssT1I CCWWGG 2 cut(s) 540, 735
Bst2BI CACGAG 1 cut(s) 1334
Bst2UI CCWGG 3 cut(s) 419, 753, 1597
Bst4CI ACNGT 8 cut(s) 10, 554, 793, 1104, 1187, 1461, 1648, 1801
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 9 cut(s) 272, 453, 771, 1140, 1386, 1467, 1654, 1701, 1755
BstDEI CTNAG 3 cut(s) 74, 530, 1704
BstDSI CCRYGG 3 cut(s) 173, 540, 735
BstENI CCTNNNNNAGG 3 cut(s) 665, 756, 1353
BstF5I GGATG 6 cut(s) 517, 1009, 1071, 1081, 1156, 1167
BstH2I RGCGCY 1 cut(s) 1178
BstHHI GCGC 2 cut(s) 315, 1177
BstMWI GCNNNNNNNGC 2 cut(s) 461, 1688
BstNI CCWGG 3 cut(s) 419, 753, 1597
BstNSI RCATGY 2 cut(s) 1469, 1519
BstSCI CCNGG 4 cut(s) 417, 751, 1551, 1595
BstSFI CTRYAG 4 cut(s) 300, 456, 553, 1379
BstSLI GKGCMC 1 cut(s) 1129
BstV1I GCAGC 8 cut(s) 442, 448, 470, 565, 841, 1393, 1651, 1859
BstX2I RGATCY 3 cut(s) 835, 934, 1477
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 3 cut(s) 835, 934, 1477
BsuI GTATCC 1 cut(s) 1802
BsuRI GGCC 3 cut(s) 1551, 1624, 1732
BtgI CCRYGG 3 cut(s) 173, 540, 735
BtsCI GGATG 6 cut(s) 517, 1009, 1071, 1081, 1156, 1167
BtsI GCAGTG 1 cut(s) 1582
BtsIMutI CAGTG 6 cut(s) 410, 789, 948, 1100, 1582, 1644
BveI ACCTGC 1 cut(s) 1386
Cac8I GCNNGC 9 cut(s) 272, 453, 771, 1140, 1386, 1467, 1654, 1701, 1755
CaiI CAGNNNCTG 2 cut(s) 412, 1102
CciI TCATGA 1 cut(s) 1720
CfoI GCGC 2 cut(s) 315, 1177
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 194, 415
Csp6I GTAC 3 cut(s) 152, 207, 603
CviQI GTAC 3 cut(s) 152, 207, 603
DdeI CTNAG 3 cut(s) 74, 530, 1704
DraI TTTAAA 3 cut(s) 666, 987, 1525
EaeI YGGCCR 2 cut(s) 1622, 1730
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 540, 735
Eco32I GATATC 2 cut(s) 351, 682
Eco47I GGWCC 2 cut(s) 194, 415
Eco47III AGCGCT 1 cut(s) 1176
Eco57I CTGAAG 2 cut(s) 695, 843
EcoNI CCTNNNNNAGG 3 cut(s) 665, 756, 1353
EcoRI GAATTC 2 cut(s) 392, 1071
EcoRII CCWGG 3 cut(s) 417, 751, 1595
EcoRV GATATC 2 cut(s) 351, 682
EcoT14I CCWWGG 2 cut(s) 540, 735
EcoT22I ATGCAT 2 cut(s) 1156, 1894
ErhI CCWWGG 2 cut(s) 540, 735
FaqI GGGAC 1 cut(s) 180
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 8 cut(s) 456, 459, 462, 579, 855, 1382, 1640, 1848
FokI GGATG 6 cut(s) 524, 1016, 1068, 1078, 1154, 1163
Fsp4HI GCNGC 8 cut(s) 456, 459, 462, 579, 855, 1382, 1640, 1848
FspBI CTAG 4 cut(s) 240, 600, 1350, 1814
GlaI GCGC 2 cut(s) 314, 1176
GluI GCNGC 8 cut(s) 456, 459, 462, 579, 855, 1382, 1640, 1848
GsaI CCCAGC 1 cut(s) 727
GsuI CTGGAG 2 cut(s) 951, 1128
HaeII RGCGCY 1 cut(s) 1178
HaeIII GGCC 3 cut(s) 1551, 1624, 1732
HapII CCGG 3 cut(s) 452, 1552, 1733
HhaI GCGC 2 cut(s) 315, 1177
Hin6I GCGC 2 cut(s) 313, 1175
HinP1I GCGC 2 cut(s) 313, 1175
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 6 cut(s) 251, 335, 881, 1093, 1321, 1874
HpaII CCGG 3 cut(s) 452, 1552, 1733
HphI GGTGA 2 cut(s) 818, 860
Hpy166II GTNNAC 4 cut(s) 146, 371, 880, 1127
Hpy188I TCNGA 9 cut(s) 288, 340, 382, 408, 609, 1065, 1214, 1320, 1879
Hpy188III TCNNGA 7 cut(s) 165, 632, 839, 930, 1145, 1544, 1721
Hpy8I GTNNAC 4 cut(s) 146, 371, 880, 1127
Hpy99I CGWCG 1 cut(s) 1065
HpyAV CCTTC 7 cut(s) 369, 481, 654, 719, 797, 1541, 1917
HpyCH4III ACNGT 8 cut(s) 10, 554, 793, 1104, 1187, 1461, 1648, 1801
HpyCH4IV ACGT 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 1688
HpyF3I CTNAG 3 cut(s) 74, 530, 1704
HpySE526I ACGT 1 cut(s) 320
HspAI GCGC 2 cut(s) 313, 1175
KroI GCCGGC 1 cut(s) 451
KroNI GCCGGC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 616
LmnI GCTCC 4 cut(s) 361, 599, 1147, 1830
Lsp1109I GCAGC 8 cut(s) 442, 448, 470, 565, 841, 1393, 1651, 1859
LweI GCATC 6 cut(s) 756, 1125, 1141, 1163, 1276, 1477
MaeI CTAG 4 cut(s) 240, 600, 1350, 1814
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 3 cut(s) 632, 906, 1033
MbiI CCGCTC 1 cut(s) 799
MboII GAAGA 8 cut(s) 139, 317, 471, 692, 836, 1880, 1883, 1951
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 3 cut(s) 835, 934, 1477
MhlI GDGCHC 1 cut(s) 1129
MlsI TGGCCA 1 cut(s) 1624
MluNI TGGCCA 1 cut(s) 1624
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 3 cut(s) 1043, 1046, 1938
Mox20I TGGCCA 1 cut(s) 1624
Mph1103I ATGCAT 2 cut(s) 1156, 1894
MroNI GCCGGC 1 cut(s) 451
MscI TGGCCA 1 cut(s) 1624
MslI CAYNNNNRTG 1 cut(s) 1633
Msp20I TGGCCA 1 cut(s) 1624
MspA1I CMGCKG 3 cut(s) 274, 461, 1783
MspI CCGG 3 cut(s) 452, 1552, 1733
MspR9I CCNGG 4 cut(s) 419, 753, 1553, 1597
MunI CAATTG 1 cut(s) 265
MvaI CCWGG 3 cut(s) 419, 753, 1597
MwoI GCNNNNNNNGC 2 cut(s) 461, 1688
NaeI GCCGGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 1553
NcoI CCATGG 2 cut(s) 540, 735
NgoMIV GCCGGC 1 cut(s) 451
NlaIV GGNNCC 2 cut(s) 196, 571
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 2 cut(s) 632, 1033
NsiI ATGCAT 2 cut(s) 1156, 1894
NspI RCATGY 2 cut(s) 1469, 1519
PaeI GCATGC 1 cut(s) 1469
PagI TCATGA 1 cut(s) 1720
PdiI GCCGGC 1 cut(s) 453
PfeI GAWTC 5 cut(s) 251, 335, 1093, 1321, 1874
PkrI GCNGC 8 cut(s) 457, 460, 463, 580, 856, 1383, 1641, 1849
PleI GAGTC 1 cut(s) 875
PpsI GAGTC 1 cut(s) 875
Psp6I CCWGG 3 cut(s) 417, 751, 1595
PspFI CCCAGC 1 cut(s) 723
PspGI CCWGG 3 cut(s) 417, 751, 1595
PspN4I GGNNCC 2 cut(s) 196, 571
PspPI GGNCC 2 cut(s) 194, 415
PstI CTGCAG 3 cut(s) 304, 460, 1383
PstNI CAGNNNCTG 2 cut(s) 412, 1102
PsuI RGATCY 3 cut(s) 835, 934, 1477
PvuII CAGCTG 2 cut(s) 461, 1783
RsaI GTAC 3 cut(s) 153, 208, 604
RsaNI GTAC 3 cut(s) 152, 207, 603
RseI CAYNNNNRTG 1 cut(s) 1633
SatI GCNGC 8 cut(s) 456, 459, 462, 579, 855, 1382, 1640, 1848
Sau96I GGNCC 2 cut(s) 194, 415
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 4 cut(s) 419, 753, 1553, 1597
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 6 cut(s) 756, 1125, 1141, 1163, 1276, 1477
SfcI CTRYAG 4 cut(s) 300, 456, 553, 1379
SinI GGWCC 2 cut(s) 194, 415
SmiMI CAYNNNNRTG 1 cut(s) 1633
SmlI CTYRAG 1 cut(s) 1694
SmoI CTYRAG 1 cut(s) 1694
SphI GCATGC 1 cut(s) 1469
SsiI CCGC 4 cut(s) 274, 688, 797, 1001
SspI AATATT 2 cut(s) 1220, 1739
SspMI CTAG 4 cut(s) 240, 600, 1350, 1814
StyD4I CCNGG 4 cut(s) 417, 751, 1551, 1595
StyI CCWWGG 2 cut(s) 540, 735
TaaI ACNGT 8 cut(s) 10, 554, 793, 1104, 1187, 1461, 1648, 1801
TaiI ACGT 1 cut(s) 323
TaqI TCGA 3 cut(s) 440, 801, 1665
TatI WGTACW 1 cut(s) 602
TfiI GAWTC 5 cut(s) 251, 335, 1093, 1321, 1874
TscAI CASTG 6 cut(s) 417, 796, 948, 1107, 1582, 1651
TseFI GTSAC 2 cut(s) 632, 1033
TseI GCWGC 8 cut(s) 455, 458, 461, 578, 854, 1381, 1639, 1847
Tsp45I GTSAC 2 cut(s) 632, 1033
TspGWI ACGGA 2 cut(s) 139, 306
TspRI CASTG 6 cut(s) 417, 796, 948, 1107, 1582, 1651
VneI GTGCAC 1 cut(s) 1125
VpaK11BI GGWCC 2 cut(s) 194, 415
XagI CCTNNNNNAGG 3 cut(s) 665, 756, 1353
XapI RAATTY 4 cut(s) 392, 475, 1071, 1251
XceI RCATGY 2 cut(s) 1469, 1519
XcmI CCANNNNNNNNNTGG 1 cut(s) 1632
XspI CTAG 4 cut(s) 240, 600, 1350, 1814
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 2 cut(s) 1156, 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.