Rorug03G0029400

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005987
Physical Location & Seq
Reverse (-)
2360004 .. 2362110
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0029400.1

Sequence Viewer

Length: 1539 bp
ATGTCGTACGAGGGATACCTGCAAAACAGCCTCATCAAGATGTATCTGGAGAAAGGACAAATGGAGCTAGCTCACCAGGTTTTCCATGATCTGCCAACACCAGATAGGGTCTCATTTAATATCATGATCGTTGGTTATGGTAAGAAGGGATATTGTTTAGAAGCTCTACAACTTTTCCATGAAATGATGGATTTGGGTCTTAAACCGGATGACTTTACCATTCTAGGACTTCTTATCTCTTGTGGAGTATTAGGAGATGCTCGAATGGGAATATCTGTACATGCATGGATTGAGAGGAGGAAAACCATTACTTCTTCAAATTTGATCTTGGGTAACGCTCTTTTAGACATGTATGTGAAGTTCAATAAACTGGAGACTGCCCGTAATATTTTTTATGCTTTAGTGGAGAAAGATATTGTTTCGTGGAACACCATGATTGCAGGGTATGCTAAGGTTGGCAATTTAGAACTTGCCCACAATTTTTTCAAGCAAATGCCTAGAAGAGATCTTGTTTCTTGGAATTCATTGATTTCTGGCTATGCCAAGAGGGGTGATTATGAGAAGGTAATTAAGGTAGTCCACAATATGGTAGCAGAGAACGCGAGGCCTGATAATATCACTTTGGTTAATTTGGTCTCTGCTGCAGCAGAAATTGGAGCACTGGACCAAGGTAAATGGATGCATGGCTTGATTTTGAGAATGCAGATGAAAATAGATGCATTTTTGGGTTCTGCATTAATAGACATGTACTGCAAGGCTGGAAGCATAGAAAGAGCATTCTTGGTTTTCAGGGGATTGACAGTGAAAGATGTGACCGTATGGACAACAATGATCACTGGATTTGCATTTCATGGTCATGGAAGGAAAGCTCTGGATCTGTTTTCTGAGATGCAGAGATTTCTATTGCCAAATGGGGTGACTCTTGTTGCTGCTCTTACAGCTTGTAGTCATAGTGGACTTGTGGAAGAAGGGCTCAGCATATTCAACAATATGAAGAAAAAGTTTGATATTGAGCCCGGAATTGAGCACTATGGCTGTTTAGTGGATCTTTTAGGTCGATCAGGGAGGTTGGTAGAGGCAAAAGATGTGATTGAAAAGATGCCAATAAAACCAAGTCGAAACATGTGGGGAGCAATGCTGAGTGCTTGTAGAGCTCATAGAAACATGGAACTAGCCGAAATAGCTTCCACAGAGCTACTCAAGTTAGAGCCTGAGAAAGACGGTGGATATATATTGTTGTCTAACATATACGCTGCCTGCGGGAGGTGGAGCTCCTCGGATAAGATTAGAGAAATCATGGAAATAAGAGGAGTGAAGAAGACCGCAGGTTGCAGTAGCGTAGTTATTGATGGAGTTATCCATGACTTTGTTGCTGCGGATAAGCGGCATCCAAGGTGGGAAGAGATCTTTCTTATTTTGCAGTGTCTGAAAAGTGAAATGAAGCTAGATGATACTGGTTTCGCCAATTTTGAGCCTTGTTTCCAAGGTGGGAAACCAATGTTACTAGACTCGTGTGATTTTTTATTTTATAGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

512

Amino Acids

57.52

Weight (kDa)

6.92

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 33 - 62 8.9e-06 PPR repeat
PPR_2 PF13041 34 - 77 6.4e-10 PPR repeat family
PPR PF01535 37 - 67 8.8e-08 PPR repeat
PPR_2 PF13041 137 - 169 6.3e-08 PPR repeat family
PPR PF01535 140 - 169 2.2e-07 PPR repeat
PPR_2 PF13041 169 - 213 3.3e-09 PPR repeat family
PPR PF01535 171 - 198 8.3e-07 PPR repeat
PPR_2 PF13041 269 - 314 1.4e-06 PPR repeat family
PPR PF01535 273 - 299 4.4e-06 PPR repeat
E_motif PF20431 386 - 447 6.1e-18 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 27, 1316
AccB7I CCANNNNNTGG 1 cut(s) 586
AccII CGCG 1 cut(s) 602
AciI CCGC 4 cut(s) 1260, 1323, 1376, 1384
AclWI GGATC 2 cut(s) 882, 1053
AcsI RAATTY 2 cut(s) 319, 520
AfaI GTAC 3 cut(s) 8, 279, 749
AfiI CCNNNNNNNGG 2 cut(s) 586, 1263
AflIII ACRYGT 3 cut(s) 348, 744, 1122
AgsI TTSAA 5 cut(s) 318, 364, 487, 985, 1094
AjnI CCWGG 1 cut(s) 75
Alw21I GWGCWC 4 cut(s) 661, 1029, 1156, 1274
Alw26I GTCTC 3 cut(s) 115, 368, 640
AlwI GGATC 2 cut(s) 882, 1053
AlwNI CAGNNNCTG 1 cut(s) 1426
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 5 cut(s) 641, 644, 929, 1253, 1373
ApoI RAATTY 2 cut(s) 319, 520
AseI ATTAAT 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 664
AsuC2I CCSGG 1 cut(s) 1017
AsuHPI GGTGA 3 cut(s) 65, 563, 928
AsuNHI GCTAGC 1 cut(s) 67
AvaII GGWCC 1 cut(s) 664
BanII GRGCYC 4 cut(s) 975, 1017, 1156, 1274
BauI CACGAG 1 cut(s) 1510
BbsI GAAGAC 1 cut(s) 1325
Bbv12I GWGCWC 4 cut(s) 661, 1029, 1156, 1274
BbvI GCAGC 5 cut(s) 628, 656, 916, 1240, 1360
BccI CCATC 2 cut(s) 181, 1343
BciT130I CCWGG 1 cut(s) 77
BciVI GTATCC 1 cut(s) 8
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 1 cut(s) 1017
BcoDI GTCTC 3 cut(s) 115, 368, 640
BfaI CTAG 6 cut(s) 68, 224, 498, 1172, 1445, 1505
BfmI CTRYAG 1 cut(s) 642
BfuAI ACCTGC 2 cut(s) 27, 1316
BfuI GTATCC 1 cut(s) 8
BglII AGATCT 2 cut(s) 505, 1404
BisI GCNGC 6 cut(s) 642, 645, 930, 1254, 1374, 1385
BlpI GCTNAGC 1 cut(s) 974
BlsI GCNGC 6 cut(s) 643, 646, 931, 1255, 1375, 1386
Bme1390I CCNGG 2 cut(s) 77, 1017
Bme18I GGWCC 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 664
BmrFI CCNGG 2 cut(s) 77, 1017
BmsI GCATC 6 cut(s) 247, 669, 706, 879, 1089, 1396
BmtI GCTAGC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 1325
BpmI CTGGAG 2 cut(s) 68, 392
Bpu10I CCTNAGC 1 cut(s) 450
Bpu1102I GCTNAGC 1 cut(s) 974
BpuEI CTTGAG 1 cut(s) 1184
BpuMI CCSGG 1 cut(s) 1017
BsaI GGTCTC 2 cut(s) 115, 640
BsaJI CCNNGG 4 cut(s) 667, 1275, 1391, 1483
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 1263
Bse1I ACTGG 4 cut(s) 375, 666, 841, 1459
Bse3DI GCAATG 1 cut(s) 1140
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 667, 1275, 1391, 1483
BseGI GGATG 3 cut(s) 214, 684, 1387
BseLI CCNNNNNNNGG 2 cut(s) 586, 1263
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 4 cut(s) 876, 988, 1130, 1203
BseNI ACTGG 4 cut(s) 375, 666, 841, 1459
BseRI GAGGAG 3 cut(s) 310, 1264, 1323
BseXI GCAGC 5 cut(s) 628, 656, 916, 1240, 1360
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 1 cut(s) 607
BsiHKAI GWGCWC 4 cut(s) 661, 1029, 1156, 1274
BsiSI CCGG 2 cut(s) 206, 1017
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 2 cut(s) 586, 1263
BsmAI GTCTC 3 cut(s) 115, 368, 640
BsmI GAATGC 2 cut(s) 705, 776
BsnI GGCC 1 cut(s) 607
Bso31I GGTCTC 2 cut(s) 115, 640
Bsp1286I GDGCHC 6 cut(s) 661, 975, 1017, 1029, 1156, 1274
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
Bsp1720I GCTNAGC 1 cut(s) 974
BspACI CCGC 4 cut(s) 1260, 1323, 1376, 1384
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 4 cut(s) 877, 987, 1131, 1204
BspFNI CGCG 1 cut(s) 602
BspHI TCATGA 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 2 cut(s) 27, 1316
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 2 cut(s) 882, 1053
BspTNI GGTCTC 2 cut(s) 115, 640
BsrDI GCAATG 1 cut(s) 1140
BsrGI TGTACA 1 cut(s) 277
BsrI ACTGG 4 cut(s) 375, 666, 841, 1459
BssECI CCNNGG 4 cut(s) 667, 1275, 1391, 1483
BssMI GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
BssSI CACGAG 1 cut(s) 1510
BssT1I CCWWGG 3 cut(s) 667, 1391, 1483
Bst2BI CACGAG 1 cut(s) 1510
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 802, 817, 1223
Bst6I CTCTTC 2 cut(s) 496, 1395
BstAPI GCANNNNNTGC 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 69, 1258
BstDEI CTNAG 5 cut(s) 450, 885, 974, 1139, 1212
BstENI CCTNNNNNAGG 1 cut(s) 1261
BstF5I GGATG 3 cut(s) 214, 684, 1387
BstFNI CGCG 1 cut(s) 602
BstKTI GATC 9 cut(s) 91, 129, 327, 508, 834, 877, 1048, 1061, 1407
BstMAI GTCTC 3 cut(s) 115, 368, 640
BstMBI GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
BstMWI GCNNNNNNNGC 5 cut(s) 446, 599, 938, 1151, 1181
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 4 cut(s) 284, 352, 748, 1126
BstSCI CCNGG 2 cut(s) 75, 1015
BstSFI CTRYAG 1 cut(s) 642
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 5 cut(s) 628, 656, 916, 1240, 1360
BstV2I GAAGAC 1 cut(s) 1325
BstX2I RGATCY 4 cut(s) 505, 874, 1045, 1404
BstYI RGATCY 4 cut(s) 505, 874, 1045, 1404
BsuI GTATCC 1 cut(s) 8
BsuRI GGCC 1 cut(s) 607
BtsCI GGATG 3 cut(s) 214, 684, 1387
BtsI GCAGTG 1 cut(s) 1427
BtsIMutI CAGTG 4 cut(s) 659, 807, 834, 1427
BveI ACCTGC 2 cut(s) 27, 1316
Cac8I GCNNGC 2 cut(s) 69, 1258
CaiI CAGNNNCTG 1 cut(s) 1426
CciI TCATGA 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 664
CsiI ACCWGGT 1 cut(s) 75
Csp6I GTAC 3 cut(s) 7, 278, 748
CviQI GTAC 3 cut(s) 7, 278, 748
DdeI CTNAG 5 cut(s) 450, 885, 974, 1139, 1212
DpnI GATC 9 cut(s) 90, 128, 326, 507, 833, 876, 1047, 1060, 1406
DpnII GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
Eam1104I CTCTTC 2 cut(s) 496, 1395
EarI CTCTTC 2 cut(s) 496, 1395
Ecl136II GAGCTC 2 cut(s) 1154, 1272
Eco130I CCWWGG 3 cut(s) 667, 1391, 1483
Eco147I AGGCCT 1 cut(s) 607
Eco24I GRGCYC 4 cut(s) 975, 1017, 1156, 1274
Eco31I GGTCTC 2 cut(s) 115, 640
Eco47I GGWCC 1 cut(s) 664
Eco53kI GAGCTC 2 cut(s) 1154, 1272
EcoICRI GAGCTC 2 cut(s) 1154, 1272
EcoNI CCTNNNNNAGG 1 cut(s) 1261
EcoRI GAATTC 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 3 cut(s) 667, 1391, 1483
EcoT22I ATGCAT 3 cut(s) 286, 684, 721
EcoT38I GRGCYC 4 cut(s) 975, 1017, 1156, 1274
ErhI CCWWGG 3 cut(s) 667, 1391, 1483
FauI CCCGC 1 cut(s) 1253
FbaI TGATCA 1 cut(s) 831
Fnu4HI GCNGC 6 cut(s) 642, 645, 930, 1254, 1374, 1385
FokI GGATG 3 cut(s) 221, 691, 1374
FriOI GRGCYC 4 cut(s) 975, 1017, 1156, 1274
Fsp4HI GCNGC 6 cut(s) 642, 645, 930, 1254, 1374, 1385
FspBI CTAG 6 cut(s) 68, 224, 498, 1172, 1445, 1505
GluI GCNGC 6 cut(s) 642, 645, 930, 1254, 1374, 1385
GsuI CTGGAG 2 cut(s) 68, 392
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 206, 1017
HinfI GANTC 2 cut(s) 919, 1508
HpaII CCGG 2 cut(s) 206, 1017
HphI GGTGA 3 cut(s) 65, 563, 928
Hpy166II GTNNAC 2 cut(s) 580, 956
Hpy188I TCNGA 3 cut(s) 886, 1279, 1428
Hpy188III TCNNGA 4 cut(s) 37, 47, 124, 872
Hpy8I GTNNAC 2 cut(s) 580, 956
HpyAV CCTTC 4 cut(s) 139, 556, 855, 962
HpyCH4III ACNGT 3 cut(s) 802, 817, 1223
HpyF10VI GCNNNNNNNGC 5 cut(s) 446, 599, 938, 1151, 1181
HpyF3I CTNAG 5 cut(s) 450, 885, 974, 1139, 1212
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
LmnI GCTCC 5 cut(s) 64, 656, 1130, 1269, 1277
Lsp1109I GCAGC 5 cut(s) 628, 656, 916, 1240, 1360
LweI GCATC 6 cut(s) 247, 669, 706, 879, 1089, 1396
MabI ACCWGGT 1 cut(s) 75
MaeI CTAG 6 cut(s) 68, 224, 498, 1172, 1445, 1505
MaeIII GTNAC 4 cut(s) 332, 811, 916, 1500
MalI GATC 9 cut(s) 90, 128, 326, 507, 833, 876, 1047, 1060, 1406
MboI GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
MboII GAAGA 7 cut(s) 306, 513, 977, 1006, 1327, 1330, 1412
MflI RGATCY 4 cut(s) 505, 874, 1045, 1404
MhlI GDGCHC 6 cut(s) 661, 975, 1017, 1029, 1156, 1274
MluCI AATT 9 cut(s) 319, 460, 478, 520, 567, 628, 651, 1020, 1465
MlyI GAGTC 2 cut(s) 913, 1502
Mph1103I ATGCAT 3 cut(s) 286, 684, 721
MseI TTAA 5 cut(s) 117, 201, 570, 627, 737
MslI CAYNNNNRTG 3 cut(s) 38, 353, 855
MspI CCGG 2 cut(s) 206, 1017
MspR9I CCNGG 2 cut(s) 77, 1017
Mva1269I GAATGC 2 cut(s) 705, 776
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 5 cut(s) 446, 599, 938, 1151, 1181
NciI CCSGG 1 cut(s) 1017
NdeII GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
NheI GCTAGC 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 811, 916
NsiI ATGCAT 3 cut(s) 286, 684, 721
NspI RCATGY 4 cut(s) 284, 352, 748, 1126
PagI TCATGA 1 cut(s) 123
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 3 cut(s) 348, 744, 1122
PctI GAATGC 2 cut(s) 705, 776
Pfl23II CGTACG 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 6 cut(s) 643, 646, 931, 1255, 1375, 1386
PleI GAGTC 2 cut(s) 913, 1502
PpsI GAGTC 2 cut(s) 913, 1502
PscI ACATGT 3 cut(s) 348, 744, 1122
PshBI ATTAAT 1 cut(s) 737
Psp124BI GAGCTC 2 cut(s) 1156, 1274
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspLI CGTACG 1 cut(s) 6
PspPI GGNCC 1 cut(s) 664
PsrI GAACNNNNNNTAC 2 cut(s) 344, 376
PstI CTGCAG 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 1426
PsuI RGATCY 4 cut(s) 505, 874, 1045, 1404
RsaI GTAC 3 cut(s) 8, 279, 749
RsaNI GTAC 3 cut(s) 7, 278, 748
RseI CAYNNNNRTG 3 cut(s) 38, 353, 855
SacI GAGCTC 2 cut(s) 1156, 1274
SaqAI TTAA 5 cut(s) 117, 201, 570, 627, 737
SatI GCNGC 6 cut(s) 642, 645, 930, 1254, 1374, 1385
Sau3AI GATC 9 cut(s) 88, 126, 324, 505, 831, 874, 1045, 1058, 1404
Sau96I GGNCC 1 cut(s) 664
SchI GAGTC 2 cut(s) 913, 1502
ScrFI CCNGG 2 cut(s) 77, 1017
SduI GDGCHC 6 cut(s) 661, 975, 1017, 1029, 1156, 1274
SexAI ACCWGGT 1 cut(s) 75
SfaNI GCATC 6 cut(s) 247, 669, 706, 879, 1089, 1396
SfcI CTRYAG 1 cut(s) 642
SinI GGWCC 1 cut(s) 664
SmiMI CAYNNNNRTG 3 cut(s) 38, 353, 855
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
Sse9I AATT 9 cut(s) 319, 460, 478, 520, 567, 628, 651, 1020, 1465
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 4 cut(s) 1260, 1323, 1376, 1384
SspI AATATT 1 cut(s) 388
SspMI CTAG 6 cut(s) 68, 224, 498, 1172, 1445, 1505
SstI GAGCTC 2 cut(s) 1156, 1274
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 75, 1015
StyI CCWWGG 3 cut(s) 667, 1391, 1483
TaaI ACNGT 3 cut(s) 802, 817, 1223
TaqI TCGA 3 cut(s) 262, 1057, 1117
TasI AATT 9 cut(s) 319, 460, 478, 520, 567, 628, 651, 1020, 1465
TatI WGTACW 2 cut(s) 277, 747
TauI GCSGC 1 cut(s) 1387
Tru1I TTAA 5 cut(s) 117, 201, 570, 627, 737
Tru9I TTAA 5 cut(s) 117, 201, 570, 627, 737
TscAI CASTG 4 cut(s) 666, 807, 841, 1427
TseFI GTSAC 2 cut(s) 811, 916
TseI GCWGC 5 cut(s) 641, 644, 929, 1253, 1373
Tsp45I GTSAC 2 cut(s) 811, 916
TspDTI ATGAA 6 cut(s) 195, 513, 722, 839, 1007, 1454
TspRI CASTG 4 cut(s) 666, 807, 841, 1427
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 664
VspI ATTAAT 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 1261
XapI RAATTY 2 cut(s) 319, 520
XceI RCATGY 4 cut(s) 284, 352, 748, 1126
XspI CTAG 6 cut(s) 68, 224, 498, 1172, 1445, 1505
Zsp2I ATGCAT 3 cut(s) 286, 684, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.