MD04G1193300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007517
Physical Location & Seq
Forward (+)
28323159 .. 28325932
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1193300.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGGTTGTGGATTCTAATGGCGGTTTCAAGTGGAACAGTGATGGTGGTGAAACTTCTGGTTCTGCTTCCGAGCAGTCTCAGAATTCGTCTCTGAATTCGAAGAATGCGAAGCGTGTCACTGGTTTTGTCTCTTGTTGTGATCATATTTCAGAAGCCGCAAACAAGAGGCTCAGACGAACTCCAATGGATTCCAACAAAAAAGCTCAATTGGCTCAAACTCTTCTAAGTTTAAGTCCTCTTGGTTTGAACCTTGGGGAGACACCCCCTTTCATAGACTTAACAGAAGTGATTGCACCCAGAAAACTCAAGGCTAAGCGTTCGCCGGAAGTTAAACCCAACGCTTTTCAGTTGATGACTGAGAAGGAAAAATTGAAGGCTTCCAACTTCAATGCATCATTTATCCGAATCGGTTCATGGCAGAGAGTGGCACACAATGAAGGTGATTTGGTGGTGAAATGTTACTACGCAAAGCGAAAATTGGTGTGGGAAATCTTGGATCAGGGTTTGAAGAGCAAGATTGAAGTGCAGTGGTCTGATATTTCGGCATTGAGAGCAGTTATCGAAGAAAATGAGCCCGGGATTCTCGAAATCGAGTTGAACCAGCCACCTACGTTCCACCGAGAATTAAATCCACAGCCGAGAAAGCATACCATGTGGAATGTGGCATCAGATTTCACCAATGGTCAAGCTCTTATCCACAGGAGGCATTATGTTCAGTTTCCCCCAGGTGCCCTTGACCAACACTACGAAAAGCTCATACAATGCGAAAGCCGGTTTTTAGAGATGAGCCGAAGACCTTTCCCGAGTCCAAAATCTCCATACTTTCAGTCAGATTTCCACAATGGTCTTGCAGAATTTTCTTTCAAGTTTGATAGACACGGGGCAGAAATCCCCTCCAATCTGCAGTTCCCCTTTTTGCCATGCGTCCAGACACGTTATGATCGTACTCAACAAGTTCAAACATACGAACAGCTCAAGCCAACTCTGAGGATCAAGGATTCAACTTCACCAATATCAGTGATGGATTTTTCTTCGCCTTTAGATGAGGTGATCGGAAACCAAGCAGTTCAAAATCCACAAATGCCGGTTTACTGGGATCAAGGGATAACCCAAGCAATGACCAGTTTTTCTGATTTCTTAGGACGAGAGCAACTTCCTGGATTGGTTTCTGTTGCACAAGCGAATTCTACTATTTCTTGTCAAAGCTTCAATGTATATAATCAAGGATTAGGAAGCCCTAATCCCAACTCCCAATTGCTGAGTAATATCGAAAGCCAGCTAATGATCGACTCACAAATTGAATTCAGCAATAAACAAGTCATGCCCCAGGCCAACTCACTCATCGCATTCCCAGAACAAACGAATTATGCATTTGCAGCTGATGTAAGGCACCCAGACTATGTACAAGAACTGGCTGATAACCAAAACTTGGTTTCCGGCGCAAATGTTGACCTACTTCAGCCTCAACCGGTCAGCTGGGTGTTGCCACTACCTCAGCCAGGCATCTGGGTGGCTGCCAGGAACTCAGGGATACAAATGGCCAAGAACAACTCAGCATCTTCATCCTTCACTCAAAATTTGACAATGGAAGACTTTGCAACTTGTAACGACTTGGGCAACCGCTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

544

Amino Acids

60.99

Weight (kDa)

6.4

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 128 - 246 1e-54 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 728, 1389
AccB7I CCANNNNNTGG 1 cut(s) 1429
AciI CCGC 3 cut(s) 21, 156, 1621
AclWI GGATC 3 cut(s) 504, 998, 1104
AcoI YGGCCR 1 cut(s) 1539
AcsI RAATTY 6 cut(s) 82, 94, 854, 1183, 1301, 1576
AcuI CTGAAG 1 cut(s) 1442
AfaI GTAC 2 cut(s) 946, 1404
AfiI CCNNNNNNNGG 2 cut(s) 1429, 1499
AflIII ACRYGT 1 cut(s) 932
AgeI ACCGGT 1 cut(s) 1468
AjnI CCWGG 5 cut(s) 724, 1156, 1326, 1498, 1517
AjuI GAANNNNNNNTTGG 2 cut(s) 890, 922
AluBI AGCT 8 cut(s) 203, 689, 754, 973, 1206, 1279, 1379, 1476
AluI AGCT 8 cut(s) 203, 689, 754, 973, 1206, 1279, 1379, 1476
Alw26I GTCTC 4 cut(s) 81, 93, 133, 251
AlwI GGATC 3 cut(s) 504, 998, 1104
Ama87I CYCGRG 2 cut(s) 575, 802
AoxI GGCC 2 cut(s) 1329, 1539
ApeKI GCWGC 2 cut(s) 1376, 1514
ApoI RAATTY 6 cut(s) 82, 94, 854, 1183, 1301, 1576
AsiGI ACCGGT 1 cut(s) 1468
Asp700I GAANNNNTTC 1 cut(s) 409
AspLEI GCGC 1 cut(s) 1442
AsuC2I CCSGG 2 cut(s) 576, 577
AsuHPI GGTGA 6 cut(s) 59, 452, 463, 667, 999, 1060
AsuII TTCGAA 1 cut(s) 98
AvaI CYCGRG 2 cut(s) 575, 802
BaeGI GKGCMC 1 cut(s) 733
BalI TGGCCA 1 cut(s) 1541
BanI GGYRCC 2 cut(s) 728, 1389
BanII GRGCYC 1 cut(s) 576
BbsI GAAGAC 2 cut(s) 799, 1596
BbvCI CCTCAGC 1 cut(s) 1494
BbvI GCAGC 2 cut(s) 1388, 1501
BccI CCATC 2 cut(s) 35, 1015
BciT130I CCWGG 5 cut(s) 726, 1158, 1328, 1500, 1519
BciVI GTATCC 1 cut(s) 1524
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 2 cut(s) 576, 577
BcoDI GTCTC 4 cut(s) 81, 93, 133, 251
BfmI CTRYAG 1 cut(s) 902
BfuI GTATCC 1 cut(s) 1524
BisI GCNGC 3 cut(s) 156, 1377, 1515
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 3 cut(s) 157, 1378, 1516
Bme1390I CCNGG 7 cut(s) 576, 577, 726, 1158, 1328, 1500, 1519
BmeT110I CYCGRG 2 cut(s) 575, 802
BmiI GGNNCC 2 cut(s) 730, 1391
BmrFI CCNGG 7 cut(s) 576, 577, 726, 1158, 1328, 1500, 1519
BmrI ACTGGG 1 cut(s) 1102
BmsI GCATC 4 cut(s) 401, 674, 1512, 1565
BmuI ACTGGG 1 cut(s) 1102
BpiI GAAGAC 2 cut(s) 799, 1596
Bpu10I CCTNAGC 1 cut(s) 1494
Bpu1102I GCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 98
BpuEI CTTGAG 2 cut(s) 290, 959
BpuMI CCSGG 2 cut(s) 576, 577
BsaJI CCNNGG 4 cut(s) 250, 575, 724, 1326
BsaWI WCCGGW 1 cut(s) 1468
Bsc4I CCNNNNNNNGG 2 cut(s) 1429, 1499
Bse118I RCCGGY 3 cut(s) 771, 1084, 1468
Bse1I ACTGG 4 cut(s) 124, 1097, 1122, 1416
Bse3DI GCAATG 1 cut(s) 1122
BseBI CCWGG 5 cut(s) 726, 1158, 1328, 1500, 1519
BseDI CCNNGG 4 cut(s) 250, 575, 724, 1326
BseGI GGATG 1 cut(s) 1562
BseLI CCNNNNNNNGG 2 cut(s) 1429, 1499
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 8 cut(s) 92, 184, 348, 977, 1250, 1508, 1539, 1566
BseNI ACTGG 4 cut(s) 124, 1097, 1122, 1416
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 2 cut(s) 1388, 1501
BseYI CCCAGC 1 cut(s) 1476
BsgI GTGCAG 1 cut(s) 545
BshFI GGCC 2 cut(s) 1331, 1541
BshNI GGYRCC 2 cut(s) 728, 1389
BshTI ACCGGT 1 cut(s) 1468
BsiHKCI CYCGRG 2 cut(s) 575, 802
BsiSI CCGG 6 cut(s) 323, 576, 772, 1085, 1437, 1469
BslI CCNNNNNNNGG 2 cut(s) 1429, 1499
BsmAI GTCTC 4 cut(s) 81, 93, 133, 251
BsmBI CGTCTC 1 cut(s) 93
BsmI GAATGC 2 cut(s) 109, 1346
BsnI GGCC 2 cut(s) 1331, 1541
BsoBI CYCGRG 2 cut(s) 575, 802
Bsp119I TTCGAA 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 576, 733
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
Bsp1720I GCTNAGC 1 cut(s) 312
BspACI CCGC 3 cut(s) 21, 156, 1621
BspANI GGCC 2 cut(s) 1331, 1541
BspCNI CTCAG 8 cut(s) 91, 183, 349, 978, 1251, 1507, 1538, 1565
BspLI GGNNCC 2 cut(s) 730, 1391
BspMAI CTGCAG 1 cut(s) 906
BspPI GGATC 3 cut(s) 504, 998, 1104
BspQI GCTCTTC 1 cut(s) 503
BspT104I TTCGAA 1 cut(s) 98
BspT107I GGYRCC 2 cut(s) 728, 1389
BsrDI GCAATG 1 cut(s) 1122
BsrFI RCCGGY 3 cut(s) 771, 1084, 1468
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 4 cut(s) 124, 1097, 1122, 1416
BssAI RCCGGY 3 cut(s) 771, 1084, 1468
BssECI CCNNGG 4 cut(s) 250, 575, 724, 1326
BssMI GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 5 cut(s) 726, 1158, 1328, 1500, 1519
Bst4CI ACNGT 1 cut(s) 38
Bst6I CTCTTC 2 cut(s) 225, 503
BstAUI TGTACA 1 cut(s) 1402
BstBI TTCGAA 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 1277
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 1 cut(s) 1562
BstHHI GCGC 1 cut(s) 1442
BstKTI GATC 7 cut(s) 142, 499, 943, 993, 1053, 1099, 1287
BstMAI GTCTC 4 cut(s) 81, 93, 133, 251
BstMBI GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
BstMWI GCNNNNNNNGC 4 cut(s) 209, 551, 643, 1376
BstNI CCWGG 5 cut(s) 726, 1158, 1328, 1500, 1519
BstSCI CCNGG 7 cut(s) 574, 575, 724, 1156, 1326, 1498, 1517
BstSFI CTRYAG 1 cut(s) 902
BstSLI GKGCMC 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 1388, 1501
BstV2I GAAGAC 2 cut(s) 799, 1596
BstXI CCANNNNNNTGG 1 cut(s) 1506
BsuI GTATCC 1 cut(s) 1524
BsuRI GGCC 2 cut(s) 1331, 1541
BtgZI GCGATG 1 cut(s) 1327
BtsCI GGATG 1 cut(s) 1562
BtsI GCAGTG 1 cut(s) 533
BtsIMutI CAGTG 4 cut(s) 43, 117, 533, 1023
Cac8I GCNNGC 1 cut(s) 1277
CfoI GCGC 1 cut(s) 1442
Cfr10I RCCGGY 3 cut(s) 771, 1084, 1468
Cfr9I CCCGGG 1 cut(s) 575
CseI GACGC 1 cut(s) 913
Csp6I GTAC 2 cut(s) 945, 1403
CspAI ACCGGT 1 cut(s) 1468
CviAII CATG 4 cut(s) 414, 652, 921, 1321
CviQI GTAC 2 cut(s) 945, 1403
DpnI GATC 7 cut(s) 141, 498, 942, 992, 1052, 1098, 1286
DpnII GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
EaeI YGGCCR 1 cut(s) 1539
Eam1104I CTCTTC 2 cut(s) 225, 503
EarI CTCTTC 2 cut(s) 225, 503
Eco130I CCWWGG 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 576
Eco57I CTGAAG 1 cut(s) 1442
Eco88I CYCGRG 2 cut(s) 575, 802
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoRI GAATTC 4 cut(s) 82, 94, 1183, 1301
EcoRII CCWGG 5 cut(s) 724, 1156, 1326, 1498, 1517
EcoT14I CCWWGG 1 cut(s) 250
EcoT22I ATGCAT 2 cut(s) 394, 1372
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 250
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 4 cut(s) 417, 655, 924, 1324
FatI CATG 4 cut(s) 413, 651, 920, 1320
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 156, 1377, 1515
FokI GGATG 1 cut(s) 1549
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 3 cut(s) 156, 1377, 1515
GlaI GCGC 1 cut(s) 1441
GluI GCNGC 3 cut(s) 156, 1377, 1515
GsaI CCCAGC 1 cut(s) 1480
HaeIII GGCC 2 cut(s) 1331, 1541
HapII CCGG 6 cut(s) 323, 576, 772, 1085, 1437, 1469
HgaI GACGC 1 cut(s) 913
HhaI GCGC 1 cut(s) 1442
Hin1II CATG 4 cut(s) 417, 655, 924, 1324
Hin6I GCGC 1 cut(s) 1440
HinP1I GCGC 1 cut(s) 1440
HincII GTYRAC 1 cut(s) 1450
HindII GTYRAC 1 cut(s) 1450
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 7 cut(s) 11, 188, 405, 580, 805, 998, 1289
HpaII CCGG 6 cut(s) 323, 576, 772, 1085, 1437, 1469
HphI GGTGA 6 cut(s) 59, 452, 463, 667, 999, 1060
Hpy166II GTNNAC 2 cut(s) 1090, 1450
Hpy188III TCNNGA 3 cut(s) 584, 802, 928
Hpy8I GTNNAC 2 cut(s) 1090, 1450
HpyAV CCTTC 4 cut(s) 355, 367, 431, 1576
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4IV ACGT 2 cut(s) 611, 934
HpyCH4V TGCA 9 cut(s) 293, 392, 526, 851, 904, 1175, 1370, 1376, 1598
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 551, 643, 1376
HpySE526I ACGT 2 cut(s) 611, 934
Hsp92II CATG 4 cut(s) 417, 655, 924, 1324
HspAI GCGC 1 cut(s) 1440
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
LguI GCTCTTC 1 cut(s) 503
Lsp1109I GCAGC 2 cut(s) 1388, 1501
LweI GCATC 4 cut(s) 401, 674, 1512, 1565
MaeII ACGT 2 cut(s) 611, 934
MaeIII GTNAC 3 cut(s) 115, 458, 1604
MalI GATC 7 cut(s) 141, 498, 942, 992, 1052, 1098, 1286
MboI GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
MboII GAAGA 8 cut(s) 112, 212, 520, 575, 804, 1023, 1551, 1601
MfeI CAATTG 2 cut(s) 206, 1253
MhlI GDGCHC 2 cut(s) 576, 733
MlsI TGGCCA 1 cut(s) 1541
MluNI TGGCCA 1 cut(s) 1541
MlyI GAGTC 2 cut(s) 814, 1283
MmeI TCCRAC 2 cut(s) 216, 405
MnlI CCTC 8 cut(s) 159, 246, 696, 904, 981, 1039, 1473, 1503
Mox20I TGGCCA 1 cut(s) 1541
Mph1103I ATGCAT 2 cut(s) 394, 1372
MroXI GAANNNNTTC 1 cut(s) 409
MscI TGGCCA 1 cut(s) 1541
MseI TTAA 4 cut(s) 230, 278, 330, 626
MslI CAYNNNNRTG 1 cut(s) 1508
Msp20I TGGCCA 1 cut(s) 1541
MspA1I CMGCKG 3 cut(s) 1379, 1476, 1623
MspI CCGG 6 cut(s) 323, 576, 772, 1085, 1437, 1469
MspR9I CCNGG 7 cut(s) 576, 577, 726, 1158, 1328, 1500, 1519
MunI CAATTG 2 cut(s) 206, 1253
Mva1269I GAATGC 2 cut(s) 109, 1346
MvaI CCWGG 5 cut(s) 726, 1158, 1328, 1500, 1519
MwoI GCNNNNNNNGC 4 cut(s) 209, 551, 643, 1376
NciI CCSGG 2 cut(s) 576, 577
NdeII GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
NlaIII CATG 4 cut(s) 417, 655, 924, 1324
NlaIV GGNNCC 2 cut(s) 730, 1391
NmeAIII GCCGAG 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 115
NsiI ATGCAT 2 cut(s) 394, 1372
NspV TTCGAA 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 503
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 940
PctI GAATGC 2 cut(s) 109, 1346
PdmI GAANNNNTTC 1 cut(s) 409
PfeI GAWTC 5 cut(s) 11, 188, 405, 580, 998
PflMI CCANNNNNTGG 1 cut(s) 1429
PfoI TCCNGGA 1 cut(s) 1156
PinAI ACCGGT 1 cut(s) 1468
PkrI GCNGC 3 cut(s) 157, 1378, 1516
PleI GAGTC 2 cut(s) 813, 1283
PpsI GAGTC 2 cut(s) 813, 1283
Psp6I CCWGG 5 cut(s) 724, 1156, 1326, 1498, 1517
PspFI CCCAGC 1 cut(s) 1476
PspGI CCWGG 5 cut(s) 724, 1156, 1326, 1498, 1517
PspN4I GGNNCC 2 cut(s) 730, 1391
PstI CTGCAG 1 cut(s) 906
PvuII CAGCTG 2 cut(s) 1379, 1476
RsaI GTAC 2 cut(s) 946, 1404
RsaNI GTAC 2 cut(s) 945, 1403
RseI CAYNNNNRTG 1 cut(s) 1508
SapI GCTCTTC 1 cut(s) 503
SaqAI TTAA 4 cut(s) 230, 278, 330, 626
SatI GCNGC 3 cut(s) 156, 1377, 1515
Sau3AI GATC 7 cut(s) 139, 496, 940, 990, 1050, 1096, 1284
SchI GAGTC 2 cut(s) 814, 1283
ScrFI CCNGG 7 cut(s) 576, 577, 726, 1158, 1328, 1500, 1519
SduI GDGCHC 2 cut(s) 576, 733
SfaNI GCATC 4 cut(s) 401, 674, 1512, 1565
SfcI CTRYAG 1 cut(s) 902
SfuI TTCGAA 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 577
SmiMI CAYNNNNRTG 1 cut(s) 1508
SmlI CTYRAG 2 cut(s) 305, 974
SmoI CTYRAG 2 cut(s) 305, 974
SsiI CCGC 3 cut(s) 21, 156, 1621
StyD4I CCNGG 7 cut(s) 574, 575, 724, 1156, 1326, 1498, 1517
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 38
TaiI ACGT 2 cut(s) 614, 937
TaqI TCGA 6 cut(s) 98, 561, 585, 591, 1269, 1287
TatI WGTACW 1 cut(s) 1402
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 5 cut(s) 11, 188, 405, 580, 998
Tru1I TTAA 4 cut(s) 230, 278, 330, 626
Tru9I TTAA 4 cut(s) 230, 278, 330, 626
TscAI CASTG 4 cut(s) 43, 124, 533, 1023
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 1376, 1514
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 4 cut(s) 259, 402, 450, 1551
TspMI CCCGGG 1 cut(s) 575
TspRI CASTG 4 cut(s) 43, 124, 533, 1023
Van91I CCANNNNNTGG 1 cut(s) 1429
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 6 cut(s) 82, 94, 854, 1183, 1301, 1576
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmaI CCCGGG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 409
Zsp2I ATGCAT 2 cut(s) 394, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.