MD12G1206200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007517
Physical Location & Seq
Forward (+)
28683471 .. 28685887
2417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1206200.v1.1.491

Sequence Viewer

Length: 594 bp
ATGCTAGTTTCTGTTTTTGGGTTGATTTGGAGTTTGCATGGCGTTTTTAAGGTTCAATTGGCTCAAACTCTTCTTAGTTTAAGTCCTCTTGGTTTGAACCTTGGGGAGACACCCTCTTTCATAGACTTAACAGAAGTGACTGTGCCCGAAAAAATCAAGACTAAGCGTTCACCCGAAGTTAATCTCAATGCTTTTCAGTTAACGACCGAGAAGGAAAGGTTGAAGGCTTCTAACTTCAATGCATCGTTTATCCGAATTGGTTCATGGCAGAGAGTAGCTCACAATGAAGGTGATTTGGTGGCGAAATATTACTACGCAAAGCGAAAACTGGTGTGGGAAATCTTGGATCAGGGTTTGAAGAGCAAGATTGAAGTGCAGTGGTCTAATATTTTGGCAATGAGAGCTGTTATCAAAGAAAATGAGCCCGGCATTCTAGAAATCGAGCTGAACCAGCCACCTACGTTCCACCGGGAATCGGATCCACAGCCGAGAAAGCATACAATGTGGAATGTGGCATCAGATTTCACCAATGGTCAAGCTCTTATTCACAGGTCCTTATCTTTATTCTCTCTTCACATTTCTCCTAAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.41

Weight (kDa)

9.33

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 78 - 186 2.1e-49 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 354, 473, 486
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 6 cut(s) 56, 97, 223, 238, 358, 371
AluBI AGCT 4 cut(s) 278, 404, 445, 539
AluI AGCT 4 cut(s) 278, 404, 445, 539
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 3 cut(s) 354, 473, 486
Asp700I GAANNNNTTC 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 552
AsuC2I CCSGG 2 cut(s) 426, 470
AsuHPI GGTGA 3 cut(s) 162, 302, 517
AvaII GGWCC 1 cut(s) 552
BaeGI GKGCMC 1 cut(s) 147
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 426
BcnI CCSGG 2 cut(s) 426, 470
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 5, 434
Bme1390I CCNGG 2 cut(s) 426, 470
Bme18I GGWCC 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 2 cut(s) 426, 470
BmsI GCATC 2 cut(s) 251, 524
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 262, 294
BpuMI CCSGG 2 cut(s) 426, 470
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 333
BseSI GKGCMC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 395
Bsh1285I CGRYCG 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 207
BsiSI CCGG 2 cut(s) 426, 469
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 147, 426
Bsp143I GATC 2 cut(s) 346, 478
BspLI GGNNCC 1 cut(s) 480
BspPI GGATC 3 cut(s) 354, 473, 486
BspQI GCTCTTC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 346, 478
BssT1I CCWWGG 1 cut(s) 100
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 3 cut(s) 75, 353, 576
BstDEI CTNAG 2 cut(s) 74, 162
BstKTI GATC 2 cut(s) 349, 481
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 346, 478
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 3 cut(s) 401, 451, 493
BstSCI CCNGG 2 cut(s) 424, 468
BstSLI GKGCMC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 552
CviAII CATG 2 cut(s) 38, 264
CviJI RGCY 9 cut(s) 62, 227, 278, 404, 424, 445, 454, 487, 539
CviKI_1 RGCY 9 cut(s) 62, 227, 278, 404, 424, 445, 454, 487, 539
DdeI CTNAG 2 cut(s) 74, 162
DpnI GATC 2 cut(s) 348, 480
DpnII GATC 2 cut(s) 346, 478
Eam1104I CTCTTC 3 cut(s) 75, 353, 576
EarI CTCTTC 3 cut(s) 75, 353, 576
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 2 cut(s) 41, 267
FaiI YATR 4 cut(s) 39, 122, 265, 498
FatI CATG 2 cut(s) 37, 263
FriOI GRGCYC 1 cut(s) 426
FspBI CTAG 2 cut(s) 5, 434
HapII CCGG 2 cut(s) 426, 469
Hin1II CATG 2 cut(s) 41, 267
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 1 cut(s) 473
HpaI GTTAAC 1 cut(s) 201
HpaII CCGG 2 cut(s) 426, 469
HphI GGTGA 3 cut(s) 162, 302, 517
Hpy166II GTNNAC 2 cut(s) 170, 201
Hpy188I TCNGA 3 cut(s) 254, 478, 520
Hpy188III TCNNGA 2 cut(s) 157, 434
Hpy8I GTNNAC 2 cut(s) 170, 201
HpyAV CCTTC 3 cut(s) 205, 217, 281
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 3 cut(s) 37, 242, 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 451, 493
HpyF3I CTNAG 2 cut(s) 74, 162
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 2 cut(s) 41, 267
KspAI GTTAAC 1 cut(s) 201
Kzo9I GATC 2 cut(s) 346, 478
LguI GCTCTTC 1 cut(s) 353
LpnPI CCDG 6 cut(s) 314, 335, 439, 464, 482, 535
LweI GCATC 2 cut(s) 251, 524
MaeI CTAG 2 cut(s) 5, 434
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 348, 480
MboI GATC 2 cut(s) 346, 478
MboII GAAGA 3 cut(s) 62, 370, 563
MfeI CAATTG 1 cut(s) 56
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 147, 426
MluCI AATT 2 cut(s) 56, 255
MnlI CCTC 2 cut(s) 96, 124
Mph1103I ATGCAT 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 259
MseI TTAA 5 cut(s) 48, 80, 128, 180, 200
MspI CCGG 2 cut(s) 426, 469
MspR9I CCNGG 2 cut(s) 426, 470
MunI CAATTG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 401, 451, 493
NciI CCSGG 2 cut(s) 426, 470
NdeII GATC 2 cut(s) 346, 478
NlaIII CATG 2 cut(s) 41, 267
NlaIV GGNNCC 1 cut(s) 480
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 353
PctI GAATGC 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 473
PpuMI RGGWCCY 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 552
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 1 cut(s) 552
PspPPI RGGWCCY 1 cut(s) 552
PsuI RGATCY 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 5 cut(s) 48, 80, 128, 180, 200
Sau3AI GATC 2 cut(s) 346, 478
Sau96I GGNCC 1 cut(s) 552
ScrFI CCNGG 2 cut(s) 426, 470
SduI GDGCHC 2 cut(s) 147, 426
SfaNI GCATC 2 cut(s) 251, 524
SinI GGWCC 1 cut(s) 552
Sse9I AATT 2 cut(s) 56, 255
SspI AATATT 2 cut(s) 308, 388
SspMI CTAG 2 cut(s) 5, 434
StyD4I CCNGG 2 cut(s) 424, 468
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 464
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 1 cut(s) 221
TasI AATT 2 cut(s) 56, 255
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 5 cut(s) 48, 80, 128, 180, 200
Tru9I TTAA 5 cut(s) 48, 80, 128, 180, 200
TscAI CASTG 1 cut(s) 383
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 109, 252, 300
TspRI CASTG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 552
XbaI TCTAGA 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 259
XspI CTAG 2 cut(s) 5, 434
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.