pycom04g17130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007517
Physical Location & Seq
Forward (+)
19657856 .. 19658128
273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17130.1

Sequence Viewer

Length: 273 bp
ATGAATTATGCATTTGCAGCTGATGTAAAGCACCCAGACTATGTACAAGAAGTGGTTGATAACCAAAACTTGGTTTCCGGCGCAAATGTTGACCTACTTCAGCCTCAGCCGGTTAGCTGGGTGTTGCCACTACCTCAGCCAGGCATCTGGGTGGCTGCCAGGAACTCAAGGATACAAATGGCCAAGAACAACTCAGCATCTTCATCCTTCACTCAAAATTCAACAATGGAAGAATTTGCAACTTATAACGACTTGGCCAACCGCTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.1

Weight (kDa)

5.04

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccB7I CCANNNNNTGG 2 cut(s) 70, 264
AciI CCGC 1 cut(s) 262
AcoI YGGCCR 2 cut(s) 180, 255
AcsI RAATTY 2 cut(s) 217, 233
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 3 cut(s) 70, 140, 264
AgsI TTSAA 1 cut(s) 222
AjnI CCWGG 2 cut(s) 139, 158
AluBI AGCT 2 cut(s) 20, 117
AluI AGCT 2 cut(s) 20, 117
AoxI GGCC 2 cut(s) 180, 255
ApeKI GCWGC 2 cut(s) 17, 155
ApoI RAATTY 2 cut(s) 217, 233
AspLEI GCGC 1 cut(s) 83
BalI TGGCCA 2 cut(s) 182, 257
BbvCI CCTCAGC 2 cut(s) 105, 135
BbvI GCAGC 2 cut(s) 29, 142
BciT130I CCWGG 2 cut(s) 141, 160
BciVI GTATCC 1 cut(s) 165
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 2 cut(s) 18, 156
BlsI GCNGC 2 cut(s) 19, 157
Bme1390I CCNGG 2 cut(s) 141, 160
BmrFI CCNGG 2 cut(s) 141, 160
BmsI GCATC 2 cut(s) 153, 206
Bpu10I CCTNAGC 2 cut(s) 105, 135
BpuEI CTTGAG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 140, 264
Bse118I RCCGGY 1 cut(s) 109
BseBI CCWGG 2 cut(s) 141, 160
BseGI GGATG 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 70, 140, 264
BseMII CTCAG 3 cut(s) 119, 149, 207
BseXI GCAGC 2 cut(s) 29, 142
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 2 cut(s) 182, 257
BsiSI CCGG 2 cut(s) 78, 110
BslI CCNNNNNNNGG 3 cut(s) 70, 140, 264
BsnI GGCC 2 cut(s) 182, 257
Bsp1407I TGTACA 1 cut(s) 43
BspACI CCGC 1 cut(s) 262
BspANI GGCC 2 cut(s) 182, 257
BspCNI CTCAG 3 cut(s) 118, 148, 206
BsrFI RCCGGY 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 109
Bst2UI CCWGG 2 cut(s) 141, 160
BstAUI TGTACA 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 105, 135, 193
BstENI CCTNNNNNAGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 83
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 141, 160
BstSCI CCNGG 2 cut(s) 139, 158
BstV1I GCAGC 2 cut(s) 29, 142
BstXI CCANNNNNNTGG 1 cut(s) 147
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 2 cut(s) 182, 257
BtsCI GGATG 1 cut(s) 203
CfoI GCGC 1 cut(s) 83
Cfr10I RCCGGY 1 cut(s) 109
Csp6I GTAC 1 cut(s) 44
CviJI RGCY 8 cut(s) 20, 103, 109, 117, 139, 155, 182, 257
CviKI_1 RGCY 8 cut(s) 20, 103, 109, 117, 139, 155, 182, 257
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 3 cut(s) 105, 135, 193
EaeI YGGCCR 2 cut(s) 180, 255
Eco57I CTGAAG 1 cut(s) 83
EcoNI CCTNNNNNAGG 1 cut(s) 138
EcoRII CCWGG 2 cut(s) 139, 158
EcoT22I ATGCAT 1 cut(s) 13
FaiI YATR 3 cut(s) 9, 42, 246
Fnu4HI GCNGC 2 cut(s) 18, 156
FokI GGATG 1 cut(s) 190
Fsp4HI GCNGC 2 cut(s) 18, 156
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 18, 156
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 182, 257
HapII CCGG 2 cut(s) 78, 110
HhaI GCGC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HpaII CCGG 2 cut(s) 78, 110
Hpy166II GTNNAC 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 217
HpyCH4V TGCA 3 cut(s) 11, 17, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 105, 135, 193
HspAI GCGC 1 cut(s) 81
Lsp1109I GCAGC 2 cut(s) 29, 142
LweI GCATC 2 cut(s) 153, 206
MboII GAAGA 2 cut(s) 192, 242
MlsI TGGCCA 2 cut(s) 182, 257
MluCI AATT 3 cut(s) 4, 217, 233
MluNI TGGCCA 2 cut(s) 182, 257
MnlI CCTC 2 cut(s) 114, 144
Mox20I TGGCCA 2 cut(s) 182, 257
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 2 cut(s) 182, 257
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 2 cut(s) 182, 257
MspA1I CMGCKG 2 cut(s) 20, 264
MspI CCGG 2 cut(s) 78, 110
MspR9I CCNGG 2 cut(s) 141, 160
MvaI CCWGG 2 cut(s) 141, 160
MwoI GCNNNNNNNGC 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 13
PflMI CCANNNNNTGG 2 cut(s) 70, 264
PkrI GCNGC 2 cut(s) 19, 157
PsiI TTATAA 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 139, 158
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 139, 158
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 1 cut(s) 149
SatI GCNGC 2 cut(s) 18, 156
ScrFI CCNGG 2 cut(s) 141, 160
SetI ASST 4 cut(s) 22, 96, 119, 136
SfaNI GCATC 2 cut(s) 153, 206
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 3 cut(s) 4, 217, 233
SsiI CCGC 1 cut(s) 262
StyD4I CCNGG 2 cut(s) 139, 158
TasI AATT 3 cut(s) 4, 217, 233
TatI WGTACW 1 cut(s) 43
TseI GCWGC 2 cut(s) 17, 155
TspDTI ATGAA 2 cut(s) 17, 192
Van91I CCANNNNNTGG 2 cut(s) 70, 264
XagI CCTNNNNNAGG 1 cut(s) 138
XapI RAATTY 2 cut(s) 217, 233
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.