MD04G1193400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011316
Physical Location & Seq
Reverse (-)
28325969 .. 28328617
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1193400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGGTTCTTCTCCGGTGATAACCCCAGAAGACGTATTGTTGTCTCTGATGAATGATGGCACCATTGATTCTCTCAGATTAAAGATCATCAATCAGCTTAAATCCAATGAGGAGCTGAAGAACACGACTATTAAAATGGCGGAGCAGAGCAAAGTTCTCAACACTCCTGGGGCCGAAAAACAGACAAAAAGAGAGCTTTTTGACGCATTAAGGCAGGAACTTGAAACCCAAGTGCTCGAAAAGGCCTCTAAGTCCGTTTGGGAGTTGATTTTAGACAACAATGGATTGGGGAATGAAATCAGCGGAACGGTTGAAAGGGTTTTCTGCCGGTTGAGCGGCCGCGAACCGCCTCTCTTTCCTCCTCCGGCGGATGCCAACAATGCTGTTGCCAGTGTCAATGCTAATGCAGATACCAGTGCCAATGCCAGTGCCAGTGCCAATGACAAGGAGAACGGAAAAGAGAAAGGAAAAACAAAAGGGAAGGCAACAGCAACAGCAAGCAATTCACATGAGAAGGAGAAAGCGCCAAAGAAGCGAAGATACAGTGAGATAAATGCACAAGGACAAGGAGAGGAAAATCAAGTTGCAGCAAAGTCTGATGAACCTCCAGCTGCTATGCCTGAAGAGTCACAAGTTCTGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.21

Weight (kDa)

5.48

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56420
fragaria_vesca FvH4_6g07400
malus_domestica MD04G1193400.v1.1
prunus_persica Prupe.6G313500_v2.0.a1
pyrus_communis pycom04g17140
rosa_chinensis RchiOBHm_Chr3g0457001 RchiOBHm_Chr5g0001621
rosa_laevigata RLG00000025253 RLG00000030950
rosa_roxburghii Rroxscaffold_6G00422280
rosa_rugosa Rorug03G0018200 Rorug03G0018300 Rorug07G0327900
rosa_samantha Rh3AG078100 Rh3BG080000 Rh3CG079900 Rh7BG453900 Rh7CG499300 Rh7DG467400
rosa_wichuraiana Rw3G006700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 336
AccII CGCG 1 cut(s) 342
AciI CCGC 6 cut(s) 140, 303, 336, 340, 347, 368
AcoI YGGCCR 1 cut(s) 337
AcuI CTGAAG 2 cut(s) 137, 642
AgsI TTSAA 2 cut(s) 224, 314
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 4 cut(s) 97, 115, 196, 611
AluI AGCT 4 cut(s) 97, 115, 196, 611
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 48
AoxI GGCC 3 cut(s) 171, 243, 337
ApeKI GCWGC 2 cut(s) 587, 611
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 28
BanI GGYRCC 1 cut(s) 59
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 598, 599
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 527
BisI GCNGC 4 cut(s) 337, 340, 588, 612
BlsI GCNGC 4 cut(s) 338, 341, 589, 613
Bme1390I CCNGG 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 2 cut(s) 61, 172
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 13
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 4 cut(s) 390, 414, 426, 432
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 1 cut(s) 376
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 4 cut(s) 390, 414, 426, 432
BseRI GAGGAG 2 cut(s) 125, 351
BseX3I CGGCCG 1 cut(s) 337
BseXI GCAGC 2 cut(s) 598, 599
Bsh1236I CGCG 1 cut(s) 342
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 3 cut(s) 173, 245, 339
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 3 cut(s) 14, 328, 365
BsmAI GTCTC 1 cut(s) 48
BsnI GGCC 3 cut(s) 173, 245, 339
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 84
BspACI CCGC 6 cut(s) 140, 303, 336, 340, 347, 368
BspANI GGCC 3 cut(s) 173, 245, 339
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 61, 172
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 4 cut(s) 390, 414, 426, 432
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 310, 545
Bst6I CTCTTC 1 cut(s) 618
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 2 cut(s) 74, 249
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 84
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 3 cut(s) 333, 380, 532
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 598, 599
BstV2I GAAGAC 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 337
BsuRI GGCC 3 cut(s) 173, 245, 339
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 5 cut(s) 397, 421, 433, 439, 550
Cac8I GCNNGC 1 cut(s) 499
CciNI GCGGCCGC 1 cut(s) 337
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 171
CseI GACGC 1 cut(s) 212
CviAII CATG 1 cut(s) 509
CviJI RGCY 7 cut(s) 97, 115, 173, 196, 245, 339, 611
CviKI_1 RGCY 7 cut(s) 97, 115, 173, 196, 245, 339, 611
DdeI CTNAG 2 cut(s) 74, 249
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 337
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
EciI GGCGGA 2 cut(s) 155, 383
EclXI CGGCCG 1 cut(s) 337
Eco147I AGGCCT 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 137, 642
EcoRII CCWGG 1 cut(s) 166
FaeI CATG 1 cut(s) 512
FaiI YATR 2 cut(s) 510, 617
FatI CATG 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 337, 340, 588, 612
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 4 cut(s) 337, 340, 588, 612
GlaI GCGC 1 cut(s) 525
GluI GCNGC 4 cut(s) 337, 340, 588, 612
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 3 cut(s) 173, 245, 339
HapII CCGG 3 cut(s) 14, 328, 365
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 526
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 2 cut(s) 68, 626
HpaII CCGG 3 cut(s) 14, 328, 365
HphI GGTGA 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 48, 77, 598, 639
HpyAV CCTTC 2 cut(s) 475, 508
HpyCH4III ACNGT 2 cut(s) 310, 545
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 3 cut(s) 407, 557, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 333, 380, 532
HpyF3I CTNAG 2 cut(s) 74, 249
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 112, 142
Lsp1109I GCAGC 2 cut(s) 598, 599
LweI GCATC 1 cut(s) 361
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 627
MalI GATC 1 cut(s) 86
MbiI CCGCTC 1 cut(s) 336
MboI GATC 1 cut(s) 84
MboII GAAGA 4 cut(s) 41, 130, 549, 635
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 1 cut(s) 502
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 7 cut(s) 103, 256, 360, 369, 372, 565, 615
MseI TTAA 4 cut(s) 80, 99, 132, 209
MspA1I CMGCKG 2 cut(s) 303, 611
MspI CCGG 3 cut(s) 14, 328, 365
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 333, 380, 532
NdeII GATC 1 cut(s) 84
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 2 cut(s) 61, 172
NmuCI GTSAC 1 cut(s) 627
NotI GCGGCCGC 1 cut(s) 337
PceI AGGCCT 1 cut(s) 245
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 4 cut(s) 338, 341, 589, 613
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 61, 172
PspPI GGNCC 1 cut(s) 171
PvuII CAGCTG 1 cut(s) 611
SaqAI TTAA 4 cut(s) 80, 99, 132, 209
SatI GCNGC 4 cut(s) 337, 340, 588, 612
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 171
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 237
SetI ASST 6 cut(s) 36, 99, 117, 198, 607, 613
SfaNI GCATC 1 cut(s) 361
Sse9I AATT 1 cut(s) 502
SseBI AGGCCT 1 cut(s) 245
SsiI CCGC 6 cut(s) 140, 303, 336, 340, 347, 368
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 310, 545
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 502
TauI GCSGC 2 cut(s) 339, 342
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 4 cut(s) 80, 99, 132, 209
Tru9I TTAA 4 cut(s) 80, 99, 132, 209
TscAI CASTG 5 cut(s) 397, 421, 433, 439, 550
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 2 cut(s) 587, 611
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 3 cut(s) 65, 309, 615
TspGWI ACGGA 2 cut(s) 244, 468
TspRI CASTG 5 cut(s) 397, 421, 433, 439, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.