MD04G1213500.v1.1

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006833
Physical Location & Seq
Reverse (-)
29717003 .. 29718663
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1213500.v1.1.491

Sequence Viewer

Length: 564 bp
ATGTTGAAGCAAGTCTTTGGGAAGGTGTGGAATAAGGGTGTTTTTGTGTACGGAGGAAACAGAGCCATGCCTTGCTTGTATGTGCCCACGAGTGATGTTCACAATGGACAGGTTCATTGTGCACCGAGAAGTTTTTTCGGGGTAGAGGATTTCCTCGATGATGACAATAGCCGGCCATATACTTACCAGAAGGAGAAGAAGTCCAAAAATCCAACAAAGCATATATCATTTAAACAGCGCACCATAGCCTACATTGAACCATTTACACTCGATGTGTTCATCTCAAAGCGGTTTGTGTCAGCCTCAATCACCCACAGGGTCACAACCAAGCAGGTTGCAACTGCCGGTACCAACTCCAAAGACATTAAAGCTGTACTCAAGTCACGTTGTGACATACCTGCCTGTTTGGCCGTCGGCCAAATTTTAGCCGATAGGGCAAGAGAAGCCGATGTCTATACCGCTGCTTATACTCCCCGGGACAGGGACAAGTTTGAAGGGAAGATTAGAGCAGTTGTTCAATCCCTCATTGATAATGGGATTGATGTTAAAGTTTATCTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

188

Amino Acids

21.06

Weight (kDa)

9.41

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 65 - 181 1.4e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 322, 406
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 347
AciI CCGC 2 cut(s) 289, 459
AcoI YGGCCR 3 cut(s) 173, 408, 415
AcsI RAATTY 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 3 cut(s) 50, 349, 375
AfiI CCNNNNNNNGG 2 cut(s) 480, 481
AgsI TTSAA 4 cut(s) 7, 257, 494, 518
AluBI AGCT 1 cut(s) 371
AluI AGCT 1 cut(s) 371
Alw21I GWGCWC 1 cut(s) 124
Alw44I GTGCAC 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 3 cut(s) 173, 408, 415
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 1 cut(s) 420
Asp718I GGTACC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 240
AsuC2I CCSGG 2 cut(s) 475, 476
AsuHPI GGTGA 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 474
BaeGI GKGCMC 2 cut(s) 87, 124
BanI GGYRCC 1 cut(s) 347
BauI CACGAG 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 448
BceAI ACGGC 1 cut(s) 395
BcnI CCSGG 2 cut(s) 475, 476
BfuAI ACCTGC 2 cut(s) 322, 406
BglI GCCNNNNNGGC 2 cut(s) 407, 434
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 475, 476
BmeT110I CYCGRG 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 475, 476
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 2 cut(s) 475, 476
BsaJI CCNNGG 2 cut(s) 473, 474
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 2 cut(s) 480, 481
Bse118I RCCGGY 2 cut(s) 171, 344
BseDI CCNNGG 2 cut(s) 473, 474
BseLI CCNNNNNNNGG 2 cut(s) 480, 481
BseSI GKGCMC 2 cut(s) 87, 124
BseXI GCAGC 1 cut(s) 448
BshFI GGCC 3 cut(s) 175, 410, 417
BshNI GGYRCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 3 cut(s) 172, 345, 475
BslFI GGGAC 2 cut(s) 491, 497
BslI CCNNNNNNNGG 2 cut(s) 480, 481
BsmFI GGGAC 2 cut(s) 491, 497
BsnI GGCC 3 cut(s) 175, 410, 417
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 87, 124
BspACI CCGC 2 cut(s) 289, 459
BspANI GGCC 3 cut(s) 175, 410, 417
BspLI GGNNCC 1 cut(s) 349
BspMI ACCTGC 2 cut(s) 322, 406
BspT107I GGYRCC 1 cut(s) 347
BsrFI RCCGGY 2 cut(s) 171, 344
BssAI RCCGGY 2 cut(s) 171, 344
BssECI CCNNGG 2 cut(s) 473, 474
BssSI CACGAG 1 cut(s) 88
Bst2BI CACGAG 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 173
BstHHI GCGC 1 cut(s) 240
BstMWI GCNNNNNNNGC 3 cut(s) 407, 434, 443
BstSCI CCNGG 2 cut(s) 473, 474
BstSLI GKGCMC 2 cut(s) 87, 124
BstV1I GCAGC 1 cut(s) 448
BsuRI GGCC 3 cut(s) 175, 410, 417
BveI ACCTGC 2 cut(s) 322, 406
Cac8I GCNNGC 1 cut(s) 173
CfoI GCGC 1 cut(s) 240
Cfr10I RCCGGY 2 cut(s) 171, 344
Cfr9I CCCGGG 1 cut(s) 474
Csp6I GTAC 3 cut(s) 49, 348, 374
CviAII CATG 1 cut(s) 67
CviQI GTAC 3 cut(s) 49, 348, 374
DraI TTTAAA 1 cut(s) 232
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 3 cut(s) 173, 408, 415
Eco88I CYCGRG 1 cut(s) 474
FaeI CATG 1 cut(s) 70
FalI AAGNNNNNCTT 2 cut(s) 31, 540
FaqI GGGAC 2 cut(s) 491, 497
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 462
Fsp4HI GCNGC 1 cut(s) 462
GlaI GCGC 1 cut(s) 239
GluI GCNGC 1 cut(s) 462
HaeIII GGCC 3 cut(s) 175, 410, 417
HapII CCGG 3 cut(s) 172, 345, 475
HhaI GCGC 1 cut(s) 240
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HpaII CCGG 3 cut(s) 172, 345, 475
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 49, 100, 122
Hpy188III TCNNGA 1 cut(s) 557
Hpy8I GTNNAC 3 cut(s) 49, 100, 122
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 3 cut(s) 16, 184, 488
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 2 cut(s) 122, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 434, 443
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 238
KpnI GGTACC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 171
KroNI GCCGGC 1 cut(s) 173
Lsp1109I GCAGC 1 cut(s) 448
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 3 cut(s) 319, 381, 389
MboII GAAGA 2 cut(s) 208, 511
MhlI GDGCHC 2 cut(s) 87, 124
MluCI AATT 1 cut(s) 420
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 5 cut(s) 47, 139, 164, 313, 533
MroNI GCCGGC 1 cut(s) 171
MseI TTAA 3 cut(s) 231, 366, 546
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 3 cut(s) 172, 345, 475
MspR9I CCNGG 2 cut(s) 475, 476
MwoI GCNNNNNNNGC 3 cut(s) 407, 434, 443
NaeI GCCGGC 1 cut(s) 173
NciI CCSGG 2 cut(s) 475, 476
NgoMIV GCCGGC 1 cut(s) 171
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 3 cut(s) 319, 381, 389
PdiI GCCGGC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 349
RsaI GTAC 3 cut(s) 50, 349, 375
RsaNI GTAC 3 cut(s) 49, 348, 374
SaqAI TTAA 3 cut(s) 231, 366, 546
SatI GCNGC 1 cut(s) 462
ScrFI CCNGG 2 cut(s) 475, 476
SduI GDGCHC 2 cut(s) 87, 124
SetI ASST 6 cut(s) 27, 114, 336, 373, 388, 400
SmaI CCCGGG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 1 cut(s) 420
SsiI CCGC 2 cut(s) 289, 459
StyD4I CCNGG 2 cut(s) 473, 474
TaiI ACGT 1 cut(s) 388
TaqI TCGA 2 cut(s) 156, 270
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 373
Tru1I TTAA 3 cut(s) 231, 366, 546
Tru9I TTAA 3 cut(s) 231, 366, 546
TseFI GTSAC 3 cut(s) 319, 381, 389
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 3 cut(s) 319, 381, 389
TspDTI ATGAA 2 cut(s) 104, 268
TspGWI ACGGA 1 cut(s) 66
TspMI CCCGGG 1 cut(s) 474
VneI GTGCAC 1 cut(s) 120
XapI RAATTY 1 cut(s) 420
XmaI CCCGGG 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.