Rorug02G0647400

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006833
Physical Location & Seq
Reverse (-)
75727646 .. 75735054
7409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0647400.1

Sequence Viewer

Length: 4908 bp
ATGATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCACCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTAACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCTCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTACTGAGAATCGAGCAGATGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATGGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCCGGGGGGCCAGCCCCATACCGTGCTGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGACGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAGACCTTCCTCTGTATCCACTATTGGGTCTGTTCCGGATCAGAATCAAAGTGTATTGGGAAAAGCTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTTGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAGGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTATGCAGTTCCTCCAGAGAAAAAAATGTGTTAAATGATGATGGTAAAGGAACTAGTGATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCGGTACTGAGGTCTCAACTATCTGTGGCCCACGGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCCTATTGTCACAGCAGTCGCTGATGGATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTTGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCATATTCCATCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAACGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATATAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTGATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACATGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCACTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTCGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTGAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTTAAATGAAGTTTCCGAAAGTAACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCGTGTTTGAGGGAGGTATCTCCAAACTGAATTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGTGTCAAGAGCAGCAGCATCGCCTATTGGAGCTCCGGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCGCCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGCAACAGCACCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAGAAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGTCAATTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCCTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCACCAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAGTTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATAGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCGCTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTGAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCATTGGGCATCACCAGGCTTACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATCACGATTGGAATCATGCTTCTCAGGAGAGACCTCAGGGAGGGTTTTATGAACCTAACTCTATGGAATTTGATAGGTTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTACTTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCATACCCTCTCACCGTCGATTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAGCAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACAGAAGACACCATTGCTTTTGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTACACAGTCATCAGAAGTTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCAGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGCTGCCTTCTACTATAACTAAAGGGTTTGACAGTGCTACACAGAGACGCCGCGTCTTATCTTCACAGGATGTTTCATCTGAGCCTGCACTCTCTCTGCCTGTCAAGCCAAGAAGCTCCATAAGCCTCAGAACTTCTGAAAAAAATCCTGCTGCCACACTGGTCTCTGAATCTCGGGCATCTAGGAAAAATGATGCACAGTTTAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCTGAGACATCCTTCATAGATGTGCTCAAGAAACCAGTCATTCCAGAGGCTGATGTAGCCAACAGGGGTGCAGTGGAGTCATCTGATGGAGGTGGCCAAGTGGGGCGAACTGGCAAGAAGAAAGGCAAGAAAGGAAGACAGATCGATCCTGCTCTCCTTGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATCGCCTTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

1635

Amino Acids

183.63

Weight (kDa)

5.63

Isoelectric Point (pI)

63.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 578 - 622 1.5e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 4 cut(s) 476, 2295, 2374, 3407
AccB7I CCANNNNNTGG 1 cut(s) 736
AccBSI CCGCTC 1 cut(s) 4329
AccII CGCG 2 cut(s) 711, 4506
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 7 cut(s) 60, 2626, 2845, 2887, 2954, 4329, 4504
AcoI YGGCCR 2 cut(s) 792, 4783
AcsI RAATTY 8 cut(s) 522, 1506, 1564, 2239, 2606, 3599, 3972, 4153
AcyI GRCGYC 1 cut(s) 4501
AfaI GTAC 3 cut(s) 1324, 2111, 4077
AflII CTTAAG 1 cut(s) 1228
AflIII ACRYGT 1 cut(s) 3459
AhdI GACNNNNNGTC 2 cut(s) 658, 4505
AhlI ACTAGT 1 cut(s) 1214
AjnI CCWGG 9 cut(s) 241, 965, 1518, 1755, 1767, 1965, 2298, 3450, 4271
AjuI GAANNNNNNNTTGG 4 cut(s) 2239, 2271, 3372, 3404
AloI GAACNNNNNNTCC 2 cut(s) 3018, 3050
Alw21I GWGCWC 3 cut(s) 2343, 2556, 4716
AlwNI CAGNNNCTG 3 cut(s) 1415, 2866, 4739
Ama87I CYCGRG 4 cut(s) 309, 1651, 2071, 4626
Aor13HI TCCGGA 1 cut(s) 904
ApaI GGGCCC 1 cut(s) 587
ApoI RAATTY 8 cut(s) 522, 1506, 1564, 2239, 2606, 3599, 3972, 4153
ArsI GACNNNNNNTTYG 2 cut(s) 3985, 4017
Asp700I GAANNNNTTC 1 cut(s) 874
AspLEI GCGC 7 cut(s) 1389, 1830, 2652, 2691, 2715, 2739, 3229
AsuC2I CCSGG 4 cut(s) 553, 751, 780, 2169
AvaI CYCGRG 4 cut(s) 309, 1651, 2071, 4626
AvaII GGWCC 4 cut(s) 840, 1760, 3419, 3982
AxyI CCTNAGG 1 cut(s) 3567
BaeGI GKGCMC 1 cut(s) 587
BaeI ACNNNNGTAYC 4 cut(s) 1314, 1347, 2093, 2126
BalI TGGCCA 2 cut(s) 794, 4785
BamHI GGATCC 1 cut(s) 3433
BanII GRGCYC 6 cut(s) 587, 1070, 1293, 2231, 2343, 2556
BarI GAAGNNNNNNTAC 2 cut(s) 944, 976
BauI CACGAG 2 cut(s) 2597, 3099
BbsI GAAGAC 6 cut(s) 522, 626, 877, 1532, 3909, 4831
Bbv12I GWGCWC 3 cut(s) 2343, 2556, 4716
BbvCI CCTCAGC 2 cut(s) 93, 2958
BceAI ACGGC 1 cut(s) 411
BcgI CGANNNNNNTGC 4 cut(s) 2522, 2556, 2963, 2997
BciT130I CCWGG 9 cut(s) 243, 967, 1520, 1757, 1769, 1967, 2300, 3452, 4273
BciVI GTATCC 2 cut(s) 895, 4870
BclI TGATCA 5 cut(s) 475, 1660, 1795, 2401, 3115
BcnI CCSGG 4 cut(s) 553, 751, 780, 2169
BcuI ACTAGT 1 cut(s) 1214
BfaI CTAG 9 cut(s) 447, 501, 1149, 1215, 3191, 3674, 3830, 4341, 4635
BfoI RGCGCY 4 cut(s) 2653, 2692, 2716, 2740
BfrI CTTAAG 1 cut(s) 1228
BfuAI ACCTGC 4 cut(s) 476, 2295, 2374, 3407
BfuI GTATCC 2 cut(s) 895, 4870
BglII AGATCT 4 cut(s) 34, 1690, 3670, 4226
BlpI GCTNAGC 2 cut(s) 201, 3879
Bme18I GGWCC 4 cut(s) 840, 1760, 3419, 3982
BmeRI GACNNNNNGTC 2 cut(s) 658, 4505
BmeT110I CYCGRG 4 cut(s) 309, 1651, 2071, 4626
BoxI GACNNNNGTC 1 cut(s) 3061
BpiI GAAGAC 6 cut(s) 522, 626, 877, 1532, 3909, 4831
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 5 cut(s) 1159, 1305, 2090, 2624, 2873
Bpu10I CCTNAGC 2 cut(s) 93, 2958
Bpu1102I GCTNAGC 2 cut(s) 201, 3879
BpuEI CTTGAG 6 cut(s) 15, 650, 1728, 2074, 2355, 4700
BpuMI CCSGG 4 cut(s) 553, 751, 780, 2169
Bsa29I ATCGAT 1 cut(s) 4833
BsaAI YACGTR 1 cut(s) 3662
BsaBI GATNNNNATC 5 cut(s) 477, 912, 987, 3543, 3727
BsaHI GRCGYC 1 cut(s) 4501
BsaI GGTCTC 5 cut(s) 1336, 1915, 2200, 3556, 4621
BsaWI WCCGGW 2 cut(s) 904, 4869
BsaXI ACNNNNNCTCC 8 cut(s) 731, 761, 3018, 3048, 4059, 4089, 4425, 4455
Bse21I CCTNAGG 1 cut(s) 3567
Bse3DI GCAATG 1 cut(s) 3909
Bse8I GATNNNNATC 5 cut(s) 477, 912, 987, 3543, 3727
BseAI TCCGGA 1 cut(s) 904
BseBI CCWGG 9 cut(s) 243, 967, 1520, 1757, 1769, 1967, 2300, 3452, 4273
BseCI ATCGAT 1 cut(s) 4833
BseJI GATNNNNATC 5 cut(s) 477, 912, 987, 3543, 3727
BseMI GCAATG 1 cut(s) 3909
BseRI GAGGAG 9 cut(s) 29, 343, 386, 389, 1977, 2012, 2218, 3026, 4272
BseSI GKGCMC 1 cut(s) 587
BseYI CCCAGC 1 cut(s) 1463
BsgI GTGCAG 5 cut(s) 759, 1077, 3826, 4524, 4779
Bsh1236I CGCG 2 cut(s) 711, 4506
BshVI ATCGAT 1 cut(s) 4833
BsiHKAI GWGCWC 3 cut(s) 2343, 2556, 4716
BsiHKCI CYCGRG 4 cut(s) 309, 1651, 2071, 4626
BsiSI CCGG 7 cut(s) 553, 750, 779, 905, 2169, 2557, 4870
BslFI GGGAC 3 cut(s) 326, 1773, 3001
BsmBI CGTCTC 1 cut(s) 4492
BsmFI GGGAC 3 cut(s) 326, 1773, 3001
BsmI GAATGC 2 cut(s) 340, 1549
Bso31I GGTCTC 5 cut(s) 1336, 1915, 2200, 3556, 4621
BsoBI CYCGRG 4 cut(s) 309, 1651, 2071, 4626
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 7 cut(s) 587, 1070, 1293, 2231, 2343, 2556, 4716
Bsp13I TCCGGA 1 cut(s) 904
Bsp1407I TGTACA 1 cut(s) 4075
Bsp1720I GCTNAGC 2 cut(s) 201, 3879
Bsp68I TCGCGA 1 cut(s) 711
BspACI CCGC 7 cut(s) 60, 2626, 2845, 2887, 2954, 4329, 4504
BspDI ATCGAT 1 cut(s) 4833
BspEI TCCGGA 1 cut(s) 904
BspFNI CGCG 2 cut(s) 711, 4506
BspHI TCATGA 3 cut(s) 3112, 4285, 4875
BspMAI CTGCAG 3 cut(s) 1260, 2712, 2871
BspMI ACCTGC 4 cut(s) 476, 2295, 2374, 3407
BspTI CTTAAG 1 cut(s) 1228
BspTNI GGTCTC 5 cut(s) 1336, 1915, 2200, 3556, 4621
BsrBI CCGCTC 1 cut(s) 4329
BsrDI GCAATG 1 cut(s) 3909
BsrGI TGTACA 1 cut(s) 4075
BssNI GRCGYC 1 cut(s) 4501
BssSI CACGAG 2 cut(s) 2597, 3099
BssT1I CCWWGG 4 cut(s) 78, 3232, 3398, 4846
Bst2BI CACGAG 2 cut(s) 2597, 3099
Bst2UI CCWGG 9 cut(s) 243, 967, 1520, 1757, 1769, 1967, 2300, 3452, 4273
Bst6I CTCTTC 2 cut(s) 1104, 1965
BstACI GRCGYC 1 cut(s) 4501
BstAFI CTTAAG 1 cut(s) 1228
BstAPI GCANNNNNTGC 2 cut(s) 1100, 2968
BstAUI TGTACA 1 cut(s) 4075
BstBAI YACGTR 1 cut(s) 3662
BstDSI CCRYGG 1 cut(s) 1349
BstENI CCTNNNNNAGG 2 cut(s) 3570, 4287
BstFNI CGCG 2 cut(s) 711, 4506
BstH2I RGCGCY 4 cut(s) 2653, 2692, 2716, 2740
BstHHI GCGC 7 cut(s) 1389, 1830, 2652, 2691, 2715, 2739, 3229
BstNI CCWGG 9 cut(s) 243, 967, 1520, 1757, 1769, 1967, 2300, 3452, 4273
BstNSI RCATGY 3 cut(s) 185, 1046, 3463
BstPAI GACNNNNGTC 1 cut(s) 3061
BstSLI GKGCMC 1 cut(s) 587
BstUI CGCG 2 cut(s) 711, 4506
BstV2I GAAGAC 6 cut(s) 522, 626, 877, 1532, 3909, 4831
BstX2I RGATCY 7 cut(s) 34, 1690, 3433, 3670, 3722, 4226, 4887
BstYI RGATCY 7 cut(s) 34, 1690, 3433, 3670, 3722, 4226, 4887
Bsu15I ATCGAT 1 cut(s) 4833
Bsu36I CCTNAGG 1 cut(s) 3567
BsuI GTATCC 2 cut(s) 895, 4870
BsuTUI ATCGAT 1 cut(s) 4833
BtgI CCRYGG 1 cut(s) 1349
BtgZI GCGATG 3 cut(s) 716, 2525, 4877
BtsI GCAGTG 6 cut(s) 1159, 1253, 2194, 3086, 4381, 4767
BtuMI TCGCGA 1 cut(s) 711
BveI ACCTGC 4 cut(s) 476, 2295, 2374, 3407
CaiI CAGNNNCTG 3 cut(s) 1415, 2866, 4739
CciI TCATGA 3 cut(s) 3112, 4285, 4875
CfoI GCGC 7 cut(s) 1389, 1830, 2652, 2691, 2715, 2739, 3229
ClaI ATCGAT 1 cut(s) 4833
CseI GACGC 5 cut(s) 2861, 2900, 4259, 4495, 4509
Csp6I GTAC 3 cut(s) 1323, 2110, 4076
CviQI GTAC 3 cut(s) 1323, 2110, 4076
DraI TTTAAA 3 cut(s) 1365, 2265, 4089
DriI GACNNNNNGTC 2 cut(s) 658, 4505
EaeI YGGCCR 2 cut(s) 792, 4783
Eam1104I CTCTTC 2 cut(s) 1104, 1965
Eam1105I GACNNNNNGTC 2 cut(s) 658, 4505
EarI CTCTTC 2 cut(s) 1104, 1965
EciI GGCGGA 2 cut(s) 49, 2943
Ecl136II GAGCTC 2 cut(s) 2341, 2554
Eco130I CCWWGG 4 cut(s) 78, 3232, 3398, 4846
Eco147I AGGCCT 1 cut(s) 2176
Eco24I GRGCYC 6 cut(s) 587, 1070, 1293, 2231, 2343, 2556
Eco31I GGTCTC 5 cut(s) 1336, 1915, 2200, 3556, 4621
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 4 cut(s) 840, 1760, 3419, 3982
Eco53kI GAGCTC 2 cut(s) 2341, 2554
Eco81I CCTNAGG 1 cut(s) 3567
Eco88I CYCGRG 4 cut(s) 309, 1651, 2071, 4626
EcoICRI GAGCTC 2 cut(s) 2341, 2554
EcoNI CCTNNNNNAGG 2 cut(s) 3570, 4287
EcoO109I RGGNCCY 4 cut(s) 583, 584, 1760, 3419
EcoRII CCWGG 9 cut(s) 241, 965, 1518, 1755, 1767, 1965, 2298, 3450, 4271
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 4 cut(s) 78, 3232, 3398, 4846
EcoT22I ATGCAT 1 cut(s) 2484
EcoT38I GRGCYC 6 cut(s) 587, 1070, 1293, 2231, 2343, 2556
ErhI CCWWGG 4 cut(s) 78, 3232, 3398, 4846
Esp3I CGTCTC 1 cut(s) 4492
FalI AAGNNNNNCTT 2 cut(s) 2239, 2271
FaqI GGGAC 3 cut(s) 326, 1773, 3001
FauI CCCGC 1 cut(s) 4322
FbaI TGATCA 5 cut(s) 475, 1660, 1795, 2401, 3115
FriOI GRGCYC 6 cut(s) 587, 1070, 1293, 2231, 2343, 2556
FspBI CTAG 9 cut(s) 447, 501, 1149, 1215, 3191, 3674, 3830, 4341, 4635
GlaI GCGC 7 cut(s) 1388, 1829, 2651, 2690, 2714, 2738, 3228
GsaI CCCAGC 1 cut(s) 1467
GsuI CTGGAG 5 cut(s) 1159, 1305, 2090, 2624, 2873
HaeII RGCGCY 4 cut(s) 2653, 2692, 2716, 2740
HapII CCGG 7 cut(s) 553, 750, 779, 905, 2169, 2557, 4870
HgaI GACGC 5 cut(s) 2861, 2900, 4259, 4495, 4509
HhaI GCGC 7 cut(s) 1389, 1830, 2652, 2691, 2715, 2739, 3229
Hin1I GRCGYC 1 cut(s) 4501
Hin6I GCGC 7 cut(s) 1387, 1828, 2650, 2689, 2713, 2737, 3227
HinP1I GCGC 7 cut(s) 1387, 1828, 2650, 2689, 2713, 2737, 3227
HincII GTYRAC 2 cut(s) 355, 652
HindII GTYRAC 2 cut(s) 355, 652
HindIII AAGCTT 2 cut(s) 2373, 3802
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 7 cut(s) 553, 750, 779, 905, 2169, 2557, 4870
Hpy166II GTNNAC 4 cut(s) 355, 652, 1619, 3665
Hpy8I GTNNAC 4 cut(s) 355, 652, 1619, 3665
Hpy99I CGWCG 3 cut(s) 363, 3756, 3894
HpyCH4IV ACGT 3 cut(s) 694, 2139, 3661
HpySE526I ACGT 3 cut(s) 694, 2139, 3661
Hsp92I GRCGYC 1 cut(s) 4501
HspAI GCGC 7 cut(s) 1387, 1828, 2650, 2689, 2713, 2737, 3227
Kpn2I TCCGGA 1 cut(s) 904
Ksp22I TGATCA 5 cut(s) 475, 1660, 1795, 2401, 3115
KspAI GTTAAC 1 cut(s) 355
MaeI CTAG 9 cut(s) 447, 501, 1149, 1215, 3191, 3674, 3830, 4341, 4635
MaeII ACGT 3 cut(s) 694, 2139, 3661
MbiI CCGCTC 1 cut(s) 4329
MfeI CAATTG 3 cut(s) 2630, 2915, 2942
MflI RGATCY 7 cut(s) 34, 1690, 3433, 3670, 3722, 4226, 4887
MhlI GDGCHC 7 cut(s) 587, 1070, 1293, 2231, 2343, 2556, 4716
MlsI TGGCCA 2 cut(s) 794, 4785
MluNI TGGCCA 2 cut(s) 794, 4785
MlyI GAGTC 5 cut(s) 134, 444, 599, 2009, 4775
Mox20I TGGCCA 2 cut(s) 794, 4785
Mph1103I ATGCAT 1 cut(s) 2484
MroI TCCGGA 1 cut(s) 904
MroXI GAANNNNTTC 1 cut(s) 874
MscI TGGCCA 2 cut(s) 794, 4785
MslI CAYNNNNRTG 4 cut(s) 172, 577, 3873, 4709
Msp20I TGGCCA 2 cut(s) 794, 4785
MspA1I CMGCKG 6 cut(s) 2216, 2626, 2707, 2845, 2866, 2887
MspCI CTTAAG 1 cut(s) 1228
MspI CCGG 7 cut(s) 553, 750, 779, 905, 2169, 2557, 4870
MunI CAATTG 3 cut(s) 2630, 2915, 2942
Mva1269I GAATGC 2 cut(s) 340, 1549
MvaI CCWGG 9 cut(s) 243, 967, 1520, 1757, 1769, 1967, 2300, 3452, 4273
MvnI CGCG 2 cut(s) 711, 4506
NciI CCSGG 4 cut(s) 553, 751, 780, 2169
NmuCI GTSAC 4 cut(s) 114, 165, 436, 1402
NruI TCGCGA 1 cut(s) 711
NsiI ATGCAT 1 cut(s) 2484
NspI RCATGY 3 cut(s) 185, 1046, 3463
PaeR7I CTCGAG 2 cut(s) 1651, 2071
PagI TCATGA 3 cut(s) 3112, 4285, 4875
PaqCI CACCTGC 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 966
PceI AGGCCT 1 cut(s) 2176
PciI ACATGT 1 cut(s) 3459
PctI GAATGC 2 cut(s) 340, 1549
PdmI GAANNNNTTC 1 cut(s) 874
PflMI CCANNNNNTGG 1 cut(s) 736
PfoI TCCNGGA 3 cut(s) 551, 1965, 2167
PleI GAGTC 5 cut(s) 133, 443, 599, 2008, 4774
PpsI GAGTC 5 cut(s) 133, 443, 599, 2008, 4774
Ppu21I YACGTR 1 cut(s) 3662
PpuMI RGGWCCY 2 cut(s) 1760, 3419
PscI ACATGT 1 cut(s) 3459
PshAI GACNNNNGTC 1 cut(s) 3061
Psp124BI GAGCTC 2 cut(s) 2343, 2556
Psp5II RGGWCCY 2 cut(s) 1760, 3419
Psp6I CCWGG 9 cut(s) 241, 965, 1518, 1755, 1767, 1965, 2298, 3450, 4271
PspFI CCCAGC 1 cut(s) 1463
PspGI CCWGG 9 cut(s) 241, 965, 1518, 1755, 1767, 1965, 2298, 3450, 4271
PspOMI GGGCCC 1 cut(s) 583
PspPPI RGGWCCY 2 cut(s) 1760, 3419
PspXI VCTCGAGB 1 cut(s) 1651
PstI CTGCAG 3 cut(s) 1260, 2712, 2871
PstNI CAGNNNCTG 3 cut(s) 1415, 2866, 4739
PsuI RGATCY 7 cut(s) 34, 1690, 3433, 3670, 3722, 4226, 4887
PvuII CAGCTG 3 cut(s) 2216, 2707, 2866
RruI TCGCGA 1 cut(s) 711
RsaI GTAC 3 cut(s) 1324, 2111, 4077
RsaNI GTAC 3 cut(s) 1323, 2110, 4076
RseI CAYNNNNRTG 4 cut(s) 172, 577, 3873, 4709
SacI GAGCTC 2 cut(s) 2343, 2556
SchI GAGTC 5 cut(s) 134, 444, 599, 2009, 4775
SduI GDGCHC 7 cut(s) 587, 1070, 1293, 2231, 2343, 2556, 4716
Sfr274I CTCGAG 2 cut(s) 1651, 2071
SinI GGWCC 4 cut(s) 840, 1760, 3419, 3982
SlaI CTCGAG 2 cut(s) 1651, 2071
SmiMI CAYNNNNRTG 4 cut(s) 172, 577, 3873, 4709
SmlI CTYRAG 9 cut(s) 30, 629, 1228, 1651, 1743, 2053, 2071, 2370, 4715
SmoI CTYRAG 9 cut(s) 30, 629, 1228, 1651, 1743, 2053, 2071, 2370, 4715
SpeI ACTAGT 1 cut(s) 1214
SseBI AGGCCT 1 cut(s) 2176
SsiI CCGC 7 cut(s) 60, 2626, 2845, 2887, 2954, 4329, 4504
SspMI CTAG 9 cut(s) 447, 501, 1149, 1215, 3191, 3674, 3830, 4341, 4635
SstI GAGCTC 2 cut(s) 2343, 2556
StuI AGGCCT 1 cut(s) 2176
StyI CCWWGG 4 cut(s) 78, 3232, 3398, 4846
TaiI ACGT 3 cut(s) 697, 2142, 3664
TatI WGTACW 1 cut(s) 4075
TauI GCSGC 3 cut(s) 2629, 2890, 4506
TseFI GTSAC 4 cut(s) 114, 165, 436, 1402
Tsp45I GTSAC 4 cut(s) 114, 165, 436, 1402
TspGWI ACGGA 2 cut(s) 27, 1366
Van91I CCANNNNNTGG 1 cut(s) 736
Vha464I CTTAAG 1 cut(s) 1228
VpaK11BI GGWCC 4 cut(s) 840, 1760, 3419, 3982
XagI CCTNNNNNAGG 2 cut(s) 3570, 4287
XapI RAATTY 8 cut(s) 522, 1506, 1564, 2239, 2606, 3599, 3972, 4153
XbaI TCTAGA 1 cut(s) 3673
XceI RCATGY 3 cut(s) 185, 1046, 3463
XcmI CCANNNNNNNNNTGG 2 cut(s) 802, 4243
XhoI CTCGAG 2 cut(s) 1651, 2071
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 9 cut(s) 447, 501, 1149, 1215, 3191, 3674, 3830, 4341, 4635
Zsp2I ATGCAT 1 cut(s) 2484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.