MD12G1228600.v1.1

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006833
Physical Location & Seq
Reverse (-)
30408123 .. 30409090
968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1228600.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCCTTGCTTGTATGTGCCCACGAGTGATGTTAACAATGGACAGGTCCACTGCGCACCGAGAAGTTTTTTCGGGGTAGAGGATTTCCTCGATGATGACAATAGCCGCCCATACACTTACCAGAAGGAGAAGAAGTCCAAAAATCCAACAAAACATATATCATTTAAACAGCGCACCGTAGCCTACATTGAACCGTTTACACTCGATGTGTTCATCTCAAAGCGGTTTGTGTCAGCCTCGATCACCCACGGGGTCACAACCAAGCAGGTTGCAACTGCCGGTACCAACTCCAAAGACATTAAAGCTGTACTCAAGTCACGTTGCGACATACCTGCCTGTTTGGCGGTCGGCCAAATTTTGGCCGATAGGGCAAGAGAAGCCGATGTTTATACTGCTGCTTATACTCCCCGGGACAGGGACAAGCTTGAAGGGAAGATTAGAGCAGTTGTTCAATCCCTCATTGACAATGGGATTGATGTTAAAGTTTATCTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

166

Amino Acids

18.41

Weight (kDa)

8.84

Isoelectric Point (pI)

28.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 43 - 159 5.4e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
Acc36I ACCTGC 2 cut(s) 256, 340
Acc65I GGTACC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 281
AccB7I CCANNNNNTGG 1 cut(s) 358
AciI CCGC 3 cut(s) 106, 223, 344
AcoI YGGCCR 2 cut(s) 349, 360
AcsI RAATTY 1 cut(s) 354
AfaI GTAC 2 cut(s) 283, 309
AfiI CCNNNNNNNGG 3 cut(s) 358, 414, 415
AgsI TTSAA 3 cut(s) 191, 428, 452
AluBI AGCT 2 cut(s) 305, 424
AluI AGCT 2 cut(s) 305, 424
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 2 cut(s) 349, 360
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 354
Asp718I GGTACC 1 cut(s) 281
AspLEI GCGC 2 cut(s) 56, 174
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 2 cut(s) 409, 410
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 1 cut(s) 281
BauI CACGAG 1 cut(s) 22
BbvI GCAGC 1 cut(s) 382
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BcnI CCSGG 2 cut(s) 409, 410
BfuAI ACCTGC 2 cut(s) 256, 340
BglI GCCNNNNNGGC 2 cut(s) 341, 368
BisI GCNGC 2 cut(s) 106, 396
BlsI GCNGC 2 cut(s) 107, 397
Bme1390I CCNGG 2 cut(s) 409, 410
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 408
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 283
BmrFI CCNGG 2 cut(s) 409, 410
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 2 cut(s) 409, 410
BsaJI CCNNGG 3 cut(s) 247, 407, 408
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 414, 415
Bse118I RCCGGY 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 247, 407, 408
BseLI CCNNNNNNNGG 3 cut(s) 358, 414, 415
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 1 cut(s) 382
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 351, 362
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 2 cut(s) 279, 409
BslFI GGGAC 2 cut(s) 425, 431
BslI CCNNNNNNNGG 3 cut(s) 358, 414, 415
BsmFI GGGAC 2 cut(s) 425, 431
BsnI GGCC 2 cut(s) 351, 362
BsoBI CYCGRG 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 1 cut(s) 240
BspACI CCGC 3 cut(s) 106, 223, 344
BspANI GGCC 2 cut(s) 351, 362
BspLI GGNNCC 1 cut(s) 283
BspMI ACCTGC 2 cut(s) 256, 340
BspT107I GGYRCC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 3 cut(s) 247, 407, 408
BssMI GATC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 22
Bst4CI ACNGT 2 cut(s) 178, 195
BstDSI CCRYGG 1 cut(s) 247
BstHHI GCGC 2 cut(s) 56, 174
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 3 cut(s) 341, 368, 377
BstSCI CCNGG 2 cut(s) 407, 408
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 351, 362
BtgI CCRYGG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
BveI ACCTGC 2 cut(s) 256, 340
CfoI GCGC 2 cut(s) 56, 174
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 46
Cfr9I CCCGGG 1 cut(s) 408
Csp6I GTAC 2 cut(s) 282, 308
CviJI RGCY 8 cut(s) 105, 182, 236, 305, 351, 362, 380, 424
CviKI_1 RGCY 8 cut(s) 105, 182, 236, 305, 351, 362, 380, 424
CviQI GTAC 2 cut(s) 282, 308
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DraI TTTAAA 1 cut(s) 166
EaeI YGGCCR 2 cut(s) 349, 360
Eco47I GGWCC 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 408
FaiI YATR 7 cut(s) 15, 112, 156, 158, 329, 390, 402
FalI AAGNNNNNCTT 1 cut(s) 474
FaqI GGGAC 2 cut(s) 425, 431
Fnu4HI GCNGC 2 cut(s) 106, 396
Fsp4HI GCNGC 2 cut(s) 106, 396
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 2 cut(s) 55, 173
GluI GCNGC 2 cut(s) 106, 396
HaeIII GGCC 2 cut(s) 351, 362
HapII CCGG 2 cut(s) 279, 409
HhaI GCGC 2 cut(s) 56, 174
Hin6I GCGC 2 cut(s) 54, 172
HinP1I GCGC 2 cut(s) 54, 172
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 422
HpaI GTTAAC 1 cut(s) 34
HpaII CCGG 2 cut(s) 279, 409
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 34, 49, 198
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 3 cut(s) 34, 49, 198
HpyAV CCTTC 2 cut(s) 118, 422
HpyCH4III ACNGT 2 cut(s) 178, 195
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 368, 377
HpySE526I ACGT 1 cut(s) 319
HspAI GCGC 2 cut(s) 54, 172
KpnI GGTACC 1 cut(s) 285
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 1 cut(s) 240
LpnPI CCDG 8 cut(s) 29, 134, 251, 292, 345, 349, 400, 422
Lsp1109I GCAGC 1 cut(s) 382
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 2 cut(s) 253, 315
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MboII GAAGA 2 cut(s) 142, 445
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 1 cut(s) 354
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 4 cut(s) 73, 98, 247, 467
MseI TTAA 4 cut(s) 33, 165, 300, 480
MspI CCGG 2 cut(s) 279, 409
MspR9I CCNGG 2 cut(s) 409, 410
MwoI GCNNNNNNNGC 3 cut(s) 341, 368, 377
NciI CCSGG 2 cut(s) 409, 410
NdeII GATC 1 cut(s) 240
NlaIV GGNNCC 1 cut(s) 283
NmuCI GTSAC 2 cut(s) 253, 315
NsbI TGCGCA 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 2 cut(s) 107, 397
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 1 cut(s) 46
RsaI GTAC 2 cut(s) 283, 309
RsaNI GTAC 2 cut(s) 282, 308
SaqAI TTAA 4 cut(s) 33, 165, 300, 480
SatI GCNGC 2 cut(s) 106, 396
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 409, 410
SduI GDGCHC 1 cut(s) 21
SetI ASST 6 cut(s) 48, 270, 307, 322, 334, 426
SinI GGWCC 1 cut(s) 46
SmaI CCCGGG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 1 cut(s) 354
SsiI CCGC 3 cut(s) 106, 223, 344
StyD4I CCNGG 2 cut(s) 407, 408
TaaI ACNGT 2 cut(s) 178, 195
TaiI ACGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 90, 204, 239
TasI AATT 1 cut(s) 354
TatI WGTACW 1 cut(s) 307
TauI GCSGC 1 cut(s) 108
Tru1I TTAA 4 cut(s) 33, 165, 300, 480
Tru9I TTAA 4 cut(s) 33, 165, 300, 480
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 253, 315
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 2 cut(s) 253, 315
TspDTI ATGAA 1 cut(s) 202
TspMI CCCGGG 1 cut(s) 408
TspRI CASTG 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 354
XmaI CCCGGG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.