MD04G1227900.v1.1

GRF1-interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007926
Physical Location & Seq
Reverse (-)
30730623 .. 30734177
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1227900.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGTAGAGGAGCAGAGCAGAGAGGTAGTAGACATATATAAAAGAGAGATGCAGCAGCACCTGATGCAGATGCAGCCCATGATGGCAGCCTATTATCCTAACAACGTCACTACTGATCACATTCAGCAGTACTTGGACGAGAACAAGTCGTTGATCCTGAAGATTGTTGAGAGCCAAAATTCTGGGAAACTGAGTGAATGTGCAGAGAACCAAGCAAAGCTGCAGCGGAATCTGATGTACCTGGCTGCCATTGCTGATTCTCAACCCCAGCCTCCCACCATGCATCCTCAGTACTCTTCCACCGGTATGATGCAACCGGGAGCACATTACATGCAGCAACAAGCGGCTCAACAGATGACACCACAATCGCTCATGGCTGCGCGCTCTTCCATGATGTACTCCCAGCAGCAGTTTTCAGCTCTACAGCAACAAGCCCTGCACAGCCAATTTGCCATGAGTGCTGGAGGAAGCGCGGGACTTCACATGCTCGGGAATGAGGGAAATAATGCTGGAGGCAGCGGGCAACTTGGGGCTGGAGGGTTTGGTGATTACGGACGCAGCTCTCCTGGAGAAGACATGCATAGGAGGATGGCTGGCGGGAGTAAGCAAGATATGGATGGGAGAGGCGGGAGCTCTGGAAGCCACGCCGGAGATGGGGGTGAGACTCTTTACCTGAAATCGACCGATGACGGGAATTAA

Protein Analysis

233

Amino Acids

25.18

Weight (kDa)

5.54

Isoelectric Point (pI)

67.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSXT PF05030 35 - 90 5.6e-21 SSXT protein (N-terminal region)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 30
AccII CGCG 2 cut(s) 382, 473
AciI CCGC 6 cut(s) 226, 344, 473, 519, 597, 627
AclWI GGATC 1 cut(s) 148
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 4 cut(s) 131, 239, 293, 398
AfiI CCNNNNNNNGG 2 cut(s) 654, 690
AgeI ACCGGT 1 cut(s) 302
AjnI CCWGG 2 cut(s) 240, 565
AluBI AGCT 4 cut(s) 220, 419, 561, 633
AluI AGCT 4 cut(s) 220, 419, 561, 633
Alw21I GWGCWC 2 cut(s) 325, 635
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 488
ApoI RAATTY 1 cut(s) 178
AsiGI ACCGGT 1 cut(s) 302
AspLEI GCGC 3 cut(s) 382, 384, 473
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 557, 671
AvaI CYCGRG 1 cut(s) 488
BanII GRGCYC 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 2 cut(s) 325, 635
BccI CCATC 4 cut(s) 76, 583, 611, 647
BciT130I CCWGG 2 cut(s) 242, 567
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 656
BfmI CTRYAG 2 cut(s) 221, 422
BmcAI AGTACT 2 cut(s) 131, 293
Bme1390I CCNGG 3 cut(s) 242, 318, 567
BmeT110I CYCGRG 1 cut(s) 488
BmrFI CCNGG 3 cut(s) 242, 318, 567
BmsI GCATC 5 cut(s) 39, 54, 60, 292, 300
BpiI GAAGAC 1 cut(s) 579
BpmI CTGGAG 4 cut(s) 483, 531, 555, 588
BpuMI CCSGG 1 cut(s) 318
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 2 cut(s) 654, 690
Bse118I RCCGGY 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 2 cut(s) 242, 567
BseGI GGATG 3 cut(s) 283, 594, 622
BseLI CCNNNNNNNGG 2 cut(s) 654, 690
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 182, 302
BsePI GCGCGC 1 cut(s) 380
BseRI GAGGAG 1 cut(s) 23
BseYI CCCAGC 2 cut(s) 267, 402
BsgI GTGCAG 2 cut(s) 222, 422
Bsh1236I CGCG 2 cut(s) 382, 473
Bsh1285I CGRYCG 1 cut(s) 684
BshTI ACCGGT 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 325, 635
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 303, 317, 648
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 654, 690
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 1 cut(s) 489
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 325, 635
Bsp143I GATC 2 cut(s) 115, 153
BspACI CCGC 6 cut(s) 226, 344, 473, 519, 597, 627
BspCNI CTCAG 2 cut(s) 183, 301
BspFNI CGCG 2 cut(s) 382, 473
BspMAI CTGCAG 1 cut(s) 225
BspPI GGATC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssHII GCGCGC 1 cut(s) 380
BssMI GATC 2 cut(s) 115, 153
Bst2UI CCWGG 2 cut(s) 242, 567
Bst6I CTCTTC 2 cut(s) 301, 391
BstAPI GCANNNNNTGC 1 cut(s) 64
BstC8I GCNNGC 3 cut(s) 382, 521, 595
BstDEI CTNAG 2 cut(s) 191, 288
BstF5I GGATG 3 cut(s) 283, 594, 622
BstFNI CGCG 2 cut(s) 382, 473
BstHHI GCGC 3 cut(s) 382, 384, 473
BstKTI GATC 2 cut(s) 118, 156
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 2 cut(s) 115, 153
BstMCI CGRYCG 1 cut(s) 684
BstMWI GCNNNNNNNGC 5 cut(s) 64, 73, 251, 458, 639
BstNI CCWGG 2 cut(s) 242, 567
BstNSI RCATGY 3 cut(s) 334, 487, 580
BstSCI CCNGG 3 cut(s) 240, 316, 565
BstSFI CTRYAG 2 cut(s) 221, 422
BstUI CGCG 2 cut(s) 382, 473
BstV2I GAAGAC 1 cut(s) 579
BstXI CCANNNNNNTGG 1 cut(s) 182
BtsCI GGATG 3 cut(s) 283, 594, 622
Cac8I GCNNGC 3 cut(s) 382, 521, 595
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 3 cut(s) 382, 384, 473
Cfr10I RCCGGY 1 cut(s) 302
CseI GACGC 1 cut(s) 564
Csp6I GTAC 4 cut(s) 130, 238, 292, 397
CspAI ACCGGT 1 cut(s) 302
CviAII CATG 8 cut(s) 79, 280, 331, 373, 391, 454, 484, 577
CviQI GTAC 4 cut(s) 130, 238, 292, 397
DdeI CTNAG 2 cut(s) 191, 288
DpnI GATC 2 cut(s) 117, 155
DpnII GATC 2 cut(s) 115, 153
Eam1104I CTCTTC 2 cut(s) 301, 391
EarI CTCTTC 2 cut(s) 301, 391
Ecl136II GAGCTC 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 635
Eco53kI GAGCTC 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 240, 565
EcoT22I ATGCAT 2 cut(s) 285, 582
EcoT38I GRGCYC 1 cut(s) 635
FaeI CATG 8 cut(s) 82, 283, 334, 376, 394, 457, 487, 580
FaqI GGGAC 1 cut(s) 489
FatI CATG 8 cut(s) 78, 279, 330, 372, 390, 453, 483, 576
FauI CCCGC 4 cut(s) 466, 512, 590, 620
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 30
FokI GGATG 3 cut(s) 270, 601, 629
FriOI GRGCYC 1 cut(s) 635
GlaI GCGC 3 cut(s) 381, 383, 472
GsaI CCCAGC 2 cut(s) 271, 406
GsuI CTGGAG 4 cut(s) 483, 531, 555, 588
HapII CCGG 3 cut(s) 303, 317, 648
HgaI GACGC 1 cut(s) 564
HhaI GCGC 3 cut(s) 382, 384, 473
Hin1II CATG 8 cut(s) 82, 283, 334, 376, 394, 457, 487, 580
Hin6I GCGC 3 cut(s) 380, 382, 471
HinP1I GCGC 3 cut(s) 380, 382, 471
HinfI GANTC 3 cut(s) 229, 257, 664
HpaII CCGG 3 cut(s) 303, 317, 648
HphI GGTGA 2 cut(s) 557, 671
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 234
Hpy188III TCNNGA 3 cut(s) 157, 490, 636
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 73, 251, 458, 639
HpyF3I CTNAG 2 cut(s) 191, 288
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 8 cut(s) 82, 283, 334, 376, 394, 457, 487, 580
HspAI GCGC 3 cut(s) 380, 382, 471
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 115, 153
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 3 cut(s) 10, 320, 630
LweI GCATC 5 cut(s) 39, 54, 60, 292, 300
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 117, 155
MboI GATC 2 cut(s) 115, 153
MboII GAAGA 4 cut(s) 172, 288, 378, 584
MhlI GDGCHC 2 cut(s) 325, 635
MluCI AATT 3 cut(s) 178, 446, 694
MlyI GAGTC 1 cut(s) 658
MnlI CCTC 9 cut(s) 16, 282, 297, 458, 490, 506, 530, 579, 617
Mph1103I ATGCAT 2 cut(s) 285, 582
MseI TTAA 1 cut(s) 697
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 226, 519
MspI CCGG 3 cut(s) 303, 317, 648
MspR9I CCNGG 3 cut(s) 242, 318, 567
MvaI CCWGG 2 cut(s) 242, 567
MvnI CGCG 2 cut(s) 382, 473
MwoI GCNNNNNNNGC 5 cut(s) 64, 73, 251, 458, 639
NciI CCSGG 1 cut(s) 318
NdeII GATC 2 cut(s) 115, 153
NlaIII CATG 8 cut(s) 82, 283, 334, 376, 394, 457, 487, 580
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 2 cut(s) 285, 582
NspI RCATGY 3 cut(s) 334, 487, 580
PauI GCGCGC 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 391
PfeI GAWTC 2 cut(s) 229, 257
PfoI TCCNGGA 1 cut(s) 565
PinAI ACCGGT 1 cut(s) 302
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
Psp124BI GAGCTC 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 240, 565
PspFI CCCAGC 2 cut(s) 267, 402
PspGI CCWGG 2 cut(s) 240, 565
PstI CTGCAG 1 cut(s) 225
PstNI CAGNNNCTG 1 cut(s) 61
PteI GCGCGC 1 cut(s) 380
RsaI GTAC 4 cut(s) 131, 239, 293, 398
RsaNI GTAC 4 cut(s) 130, 238, 292, 397
RseI CAYNNNNRTG 1 cut(s) 305
SacI GAGCTC 1 cut(s) 635
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 1 cut(s) 697
Sau3AI GATC 2 cut(s) 115, 153
ScaI AGTACT 2 cut(s) 131, 293
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 3 cut(s) 242, 318, 567
SduI GDGCHC 2 cut(s) 325, 635
SetI ASST 9 cut(s) 27, 63, 108, 222, 243, 421, 563, 635, 675
SfaNI GCATC 5 cut(s) 39, 54, 60, 292, 300
SfcI CTRYAG 2 cut(s) 221, 422
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 3 cut(s) 178, 446, 694
SsiI CCGC 6 cut(s) 226, 344, 473, 519, 597, 627
SstI GAGCTC 1 cut(s) 635
StyD4I CCNGG 3 cut(s) 240, 316, 565
TaiI ACGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 680
TaqII GACCGA 1 cut(s) 698
TasI AATT 3 cut(s) 178, 446, 694
TatI WGTACW 3 cut(s) 129, 291, 396
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 2 cut(s) 229, 257
Tru1I TTAA 1 cut(s) 697
Tru9I TTAA 1 cut(s) 697
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 567
XapI RAATTY 1 cut(s) 178
XceI RCATGY 3 cut(s) 334, 487, 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 30
ZrmI AGTACT 2 cut(s) 131, 293
Zsp2I ATGCAT 2 cut(s) 285, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.