MD12G1244500.v1.1

GRF1-interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007926
Physical Location & Seq
Forward (+)
31522031 .. 31525684
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1244500.v1.1.491

Sequence Viewer

Length: 651 bp
ATGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCCTATTATCCCAACAACGTCACTACCGATCACATTCAGCAGTACTTGGACGAGAACAAGTCGTTGATTCTGAAGATTGTTGAGAGCCAGAATAACGGGAAACTGAGTGAATGTGCAGAGAACCAAGCAAGGCTACAGCGGAATCTGATGTACCTGGCTGCCATTGCTGATTCACAACCCCAGCCTCCTACCATGCATCCTCAGTACTCTTCCACCGGCATGATGCAGCCAGGAGCACATTACATGCAGCAGCAAGCAGCTCAGCAGATGACACCACAATCGCTCATGGCTGCGCGCTCTTCCATGATGTACTCCCAGCATCCGTTTTCAGCTCTGCAGCAACAAGCCCTGCATAGCCAACTTGCTATGACTGCTGGAGGAAGCGCGGGTCTGCACATGCTCGGGAATGAGGGAAATAATGCTGGAGGGAGCGGGCAACTTGGGGCTGGAGGGTTTGGTGATTATGGGCGCAGCTCTCCTGGAGAAGGCATGCATAGGAGGATGGCCGGTGGGAGTAAGCAAGATATGGATGGGCGCGGCGGGAGCTCTGGAAGCCACGGTGGAGATGGGGGTGAGACTCTTTACCTGAAATCGACCGATGATGGAAATTAA

Protein Analysis

217

Amino Acids

23.13

Weight (kDa)

6.1

Isoelectric Point (pI)

62.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSXT PF05030 19 - 74 3.8e-21 SSXT protein (N-terminal region)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 471
AccII CGCG 3 cut(s) 334, 425, 576
AciI CCGC 5 cut(s) 178, 425, 471, 576, 579
AcoI YGGCCR 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 4 cut(s) 83, 191, 245, 350
AfiI CCNNNNNNNGG 1 cut(s) 524
AjnI CCWGG 3 cut(s) 192, 268, 517
AluBI AGCT 4 cut(s) 299, 371, 513, 585
AluI AGCT 4 cut(s) 299, 371, 513, 585
Alw21I GWGCWC 2 cut(s) 277, 587
Alw26I GTCTC 1 cut(s) 608
AlwNI CAGNNNCTG 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 1 cut(s) 543
AspLEI GCGC 5 cut(s) 334, 336, 425, 510, 576
AsuHPI GGTGA 2 cut(s) 509, 623
AvaI CYCGRG 1 cut(s) 440
BanII GRGCYC 1 cut(s) 587
Bbv12I GWGCWC 2 cut(s) 277, 587
BccI CCATC 5 cut(s) 28, 535, 563, 599, 635
BciT130I CCWGG 3 cut(s) 194, 270, 519
BcoDI GTCTC 1 cut(s) 608
BfmI CTRYAG 2 cut(s) 173, 374
BlpI GCTNAGC 1 cut(s) 300
BmcAI AGTACT 2 cut(s) 83, 245
Bme1390I CCNGG 3 cut(s) 194, 270, 519
BmeT110I CYCGRG 1 cut(s) 440
BmrFI CCNGG 3 cut(s) 194, 270, 519
BmsI GCATC 5 cut(s) 6, 12, 244, 252, 367
BpmI CTGGAG 4 cut(s) 435, 483, 507, 540
Bpu1102I GCTNAGC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 595
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse118I RCCGGY 2 cut(s) 254, 545
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 3 cut(s) 194, 270, 519
BseDI CCNNGG 1 cut(s) 595
BseGI GGATG 4 cut(s) 235, 358, 546, 574
BseLI CCNNNNNNNGG 1 cut(s) 524
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 134, 254, 314
BsePI GCGCGC 1 cut(s) 332
BseYI CCCAGC 2 cut(s) 219, 354
BsgI GTGCAG 2 cut(s) 174, 416
Bsh1236I CGCG 3 cut(s) 334, 425, 576
Bsh1285I CGRYCG 1 cut(s) 636
BshFI GGCC 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 636
BsiHKAI GWGCWC 2 cut(s) 277, 587
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 2 cut(s) 255, 546
BslI CCNNNNNNNGG 1 cut(s) 524
BsmAI GTCTC 1 cut(s) 608
BsnI GGCC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 277, 587
Bsp143I GATC 1 cut(s) 67
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 5 cut(s) 178, 425, 471, 576, 579
BspANI GGCC 1 cut(s) 545
BspCNI CTCAG 3 cut(s) 135, 253, 313
BspFNI CGCG 3 cut(s) 334, 425, 576
BspMAI CTGCAG 1 cut(s) 378
BspQI GCTCTTC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 201
BsrFI RCCGGY 2 cut(s) 254, 545
BssAI RCCGGY 2 cut(s) 254, 545
BssECI CCNNGG 1 cut(s) 595
BssHII GCGCGC 1 cut(s) 332
BssMI GATC 1 cut(s) 67
Bst2UI CCWGG 3 cut(s) 194, 270, 519
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 2 cut(s) 253, 343
BstAPI GCANNNNNTGC 1 cut(s) 16
BstC8I GCNNGC 4 cut(s) 294, 334, 473, 530
BstDEI CTNAG 3 cut(s) 143, 240, 300
BstDSI CCRYGG 1 cut(s) 595
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 235, 358, 546, 574
BstFNI CGCG 3 cut(s) 334, 425, 576
BstHHI GCGC 5 cut(s) 334, 336, 425, 510, 576
BstKTI GATC 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 1 cut(s) 67
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 6 cut(s) 16, 25, 203, 410, 582, 591
BstNI CCWGG 3 cut(s) 194, 270, 519
BstNSI RCATGY 3 cut(s) 286, 439, 532
BstSCI CCNGG 3 cut(s) 192, 268, 517
BstSFI CTRYAG 2 cut(s) 173, 374
BstUI CGCG 3 cut(s) 334, 425, 576
BsuRI GGCC 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 595
BtsCI GGATG 4 cut(s) 235, 358, 546, 574
Cac8I GCNNGC 4 cut(s) 294, 334, 473, 530
CaiI CAGNNNCTG 1 cut(s) 13
CfoI GCGC 5 cut(s) 334, 336, 425, 510, 576
Cfr10I RCCGGY 2 cut(s) 254, 545
Csp6I GTAC 4 cut(s) 82, 190, 244, 349
CviAII CATG 7 cut(s) 232, 259, 283, 325, 343, 436, 529
CviQI GTAC 4 cut(s) 82, 190, 244, 349
DdeI CTNAG 3 cut(s) 143, 240, 300
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 2 cut(s) 253, 343
EarI CTCTTC 2 cut(s) 253, 343
Ecl136II GAGCTC 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoRII CCWGG 3 cut(s) 192, 268, 517
EcoT22I ATGCAT 2 cut(s) 237, 534
EcoT38I GRGCYC 1 cut(s) 587
FaeI CATG 7 cut(s) 235, 262, 286, 328, 346, 439, 532
FatI CATG 7 cut(s) 231, 258, 282, 324, 342, 435, 528
FauI CCCGC 3 cut(s) 418, 464, 572
FokI GGATG 4 cut(s) 222, 345, 553, 581
FriOI GRGCYC 1 cut(s) 587
GlaI GCGC 5 cut(s) 333, 335, 424, 509, 575
GsaI CCCAGC 2 cut(s) 223, 358
GsuI CTGGAG 4 cut(s) 435, 483, 507, 540
HaeIII GGCC 1 cut(s) 545
HapII CCGG 2 cut(s) 255, 546
HhaI GCGC 5 cut(s) 334, 336, 425, 510, 576
Hin1II CATG 7 cut(s) 235, 262, 286, 328, 346, 439, 532
Hin6I GCGC 5 cut(s) 332, 334, 423, 508, 574
HinP1I GCGC 5 cut(s) 332, 334, 423, 508, 574
HinfI GANTC 4 cut(s) 106, 181, 209, 616
HpaII CCGG 2 cut(s) 255, 546
HphI GGTGA 2 cut(s) 509, 623
Hpy188I TCNGA 2 cut(s) 111, 186
Hpy188III TCNNGA 2 cut(s) 442, 588
HpyAV CCTTC 1 cut(s) 518
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 25, 203, 410, 582, 591
HpyF3I CTNAG 3 cut(s) 143, 240, 300
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 7 cut(s) 235, 262, 286, 328, 346, 439, 532
HspAI GCGC 5 cut(s) 332, 334, 423, 508, 574
Kzo9I GATC 1 cut(s) 67
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 272, 468, 582
LweI GCATC 5 cut(s) 6, 12, 244, 252, 367
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 58
MalI GATC 1 cut(s) 69
MbiI CCGCTC 1 cut(s) 471
MboI GATC 1 cut(s) 67
MboII GAAGA 3 cut(s) 124, 240, 330
MhlI GDGCHC 2 cut(s) 277, 587
MluCI AATT 1 cut(s) 646
MlyI GAGTC 1 cut(s) 610
MnlI CCTC 7 cut(s) 234, 249, 410, 442, 458, 482, 531
Mph1103I ATGCAT 2 cut(s) 237, 534
MseI TTAA 1 cut(s) 649
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 2 cut(s) 255, 546
MspR9I CCNGG 3 cut(s) 194, 270, 519
MvaI CCWGG 3 cut(s) 194, 270, 519
MvnI CGCG 3 cut(s) 334, 425, 576
MwoI GCNNNNNNNGC 6 cut(s) 16, 25, 203, 410, 582, 591
NdeII GATC 1 cut(s) 67
NlaIII CATG 7 cut(s) 235, 262, 286, 328, 346, 439, 532
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 2 cut(s) 237, 534
NspI RCATGY 3 cut(s) 286, 439, 532
PaeI GCATGC 1 cut(s) 532
PauI GCGCGC 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 3 cut(s) 106, 181, 209
PfoI TCCNGGA 1 cut(s) 517
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 192, 268, 517
PspFI CCCAGC 2 cut(s) 219, 354
PspGI CCWGG 3 cut(s) 192, 268, 517
PstI CTGCAG 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 13
PteI GCGCGC 1 cut(s) 332
RsaI GTAC 4 cut(s) 83, 191, 245, 350
RsaNI GTAC 4 cut(s) 82, 190, 244, 349
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 587
SapI GCTCTTC 1 cut(s) 343
SaqAI TTAA 1 cut(s) 649
Sau3AI GATC 1 cut(s) 67
ScaI AGTACT 2 cut(s) 83, 245
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 3 cut(s) 194, 270, 519
SduI GDGCHC 2 cut(s) 277, 587
SetI ASST 8 cut(s) 15, 60, 195, 301, 373, 515, 587, 627
SfaNI GCATC 5 cut(s) 6, 12, 244, 252, 367
SfcI CTRYAG 2 cut(s) 173, 374
SmiMI CAYNNNNRTG 1 cut(s) 257
SphI GCATGC 1 cut(s) 532
Sse9I AATT 1 cut(s) 646
SsiI CCGC 5 cut(s) 178, 425, 471, 576, 579
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 192, 268, 517
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 632
TaqII GACCGA 1 cut(s) 650
TasI AATT 1 cut(s) 646
TatI WGTACW 3 cut(s) 81, 243, 348
TauI GCSGC 1 cut(s) 579
TfiI GAWTC 3 cut(s) 106, 181, 209
Tru1I TTAA 1 cut(s) 649
Tru9I TTAA 1 cut(s) 649
TseFI GTSAC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 58
TspGWI ACGGA 1 cut(s) 351
XagI CCTNNNNNAGG 1 cut(s) 522
XceI RCATGY 3 cut(s) 286, 439, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
ZrmI AGTACT 2 cut(s) 83, 245
Zsp2I ATGCAT 2 cut(s) 237, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.