MD04G1245100.v1.1

Isopentenyl-diphosphate Delta-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009079
Physical Location & Seq
Reverse (-)
32086167 .. 32088962
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1245100.v1.1.491

Sequence Viewer

Length: 873 bp
ATGAGTCTCACCACCACTCGCCTTCTCCACACACTCTCCTCCTTCTTCCCCAAATCTCCTCCTCCTCTTATCTTCTCATCCGCTGCGACTCGAATCTCTTCCTTCTCCAAGCCCTCCGCCTTCCCCTCCCTCTCCGCCAGGCTATCCCTCTCTTTTCACATCTCCACCATGGGCGACGCTCCCGACGCCGGCATGGACGCCGTCCAGCGCCGCCTCATGTTTGAAGACGAATGTATTCTGGTGGATGAGAATGATCGTGTTGTAGGTCACGACACCAAGTACAATTGTCACTTGATGGAAAAGATCGAATCTGAAAATTTGCTTCACAGAGCTTTCAGTGTCTTTTTGTTTAACTCAAAGCATGAGTTGCTTCTTCAGCAACGTTCTGCAACCAAGGTAACATTTCCACTTGTGTGGACAAACACCTGTTGCAGCCATCCGCTGTATCGTGAATCCGAGCTTATTGATGAGAACTCTCTTGGGGTAAGAAATGCTGCTCAAAGGAAGCTTTTGGATGAACTCGGTATTCCTGCTGAAGATGTGCCAGTTGATCAGTTTATCCCGTTGGGTCGCATGCTGTACAAGGCACCTTCTGATGGCAAGTGGGGAGAACATGAACTTGATTACCTTCTCTTCACTGTACGTGATGTTAATGTGCATCCGAACCCTGACGAGGTAGCTGATATCAAGTACGTAAACCAGGAGCAGCTGAAAGAGCTGTTGAGGAAGGCAGATGCCGGGGAGGAAGGTCTGAAGCTGTCGCCTTGGTTCAGGCTCGTGGTGGACAACTTCTTGTTCAAGTGGTGGGACCATGTTGAGAAAAACACCATCAAGGAAGCTGCTGACATGAAAACCATCCACAGGTTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
NUDIX

Protein Analysis

291

Amino Acids

33.14

Weight (kDa)

5.78

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 108 - 243 9.7e-15 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AciI CCGC 5 cut(s) 81, 117, 135, 211, 440
AclI AACGTT 1 cut(s) 382
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 3 cut(s) 359, 555, 773
AcyI GRCGYC 2 cut(s) 186, 198
AfaI GTAC 4 cut(s) 281, 581, 642, 692
AfiI CCNNNNNNNGG 4 cut(s) 188, 596, 673, 861
AgsI TTSAA 2 cut(s) 224, 799
AjnI CCWGG 2 cut(s) 137, 699
AleI CACNNNNGTG 1 cut(s) 412
AluBI AGCT 8 cut(s) 332, 460, 508, 680, 709, 718, 757, 839
AluI AGCT 8 cut(s) 332, 460, 508, 680, 709, 718, 757, 839
Alw26I GTCTC 1 cut(s) 11
ApeKI GCWGC 5 cut(s) 83, 432, 494, 706, 839
ApoI RAATTY 1 cut(s) 316
Asp700I GAANNNNTTC 2 cut(s) 97, 234
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 808
AsuC2I CCSGG 1 cut(s) 739
AvaII GGWCC 1 cut(s) 808
BanI GGYRCC 1 cut(s) 586
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 5 cut(s) 70, 444, 481, 718, 826
BccI CCATC 5 cut(s) 289, 444, 590, 836, 863
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 2 cut(s) 139, 701
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 1 cut(s) 11
BfoI RGCGCY 1 cut(s) 211
BisI GCNGC 6 cut(s) 84, 211, 433, 495, 707, 840
BlsI GCNGC 6 cut(s) 85, 212, 434, 496, 708, 841
Bme1390I CCNGG 3 cut(s) 139, 701, 739
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 808
BmiI GGNNCC 2 cut(s) 588, 809
BmrFI CCNGG 3 cut(s) 139, 701, 739
BmsI GCATC 2 cut(s) 667, 724
BpiI GAAGAC 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 739
BsaAI YACGTR 2 cut(s) 644, 694
BsaHI GRCGYC 2 cut(s) 186, 198
BsaJI CCNNGG 4 cut(s) 168, 393, 738, 764
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 596, 673, 861
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 545
BseBI CCWGG 2 cut(s) 139, 701
BseDI CCNNGG 4 cut(s) 168, 393, 738, 764
BseGI GGATG 6 cut(s) 77, 250, 436, 520, 658, 855
BseLI CCNNNNNNNGG 4 cut(s) 188, 596, 673, 861
BseNI ACTGG 1 cut(s) 545
BseRI GAGGAG 4 cut(s) 28, 48, 51, 54
BseXI GCAGC 5 cut(s) 70, 444, 481, 718, 826
BshNI GGYRCC 1 cut(s) 586
BsiSI CCGG 2 cut(s) 189, 738
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 4 cut(s) 188, 596, 673, 861
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 821
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 3 cut(s) 253, 303, 550
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 5 cut(s) 81, 117, 135, 211, 440
BspLI GGNNCC 2 cut(s) 588, 809
BspT107I GGYRCC 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 188
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 4 cut(s) 168, 393, 738, 764
BssMI GATC 3 cut(s) 253, 303, 550
BssNI GRCGYC 2 cut(s) 186, 198
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 3 cut(s) 168, 393, 764
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 139, 701
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 2 cut(s) 103, 638
BstACI GRCGYC 2 cut(s) 186, 198
BstAPI GCANNNNNTGC 1 cut(s) 367
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 2 cut(s) 644, 694
BstC8I GCNNGC 2 cut(s) 190, 575
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 6 cut(s) 77, 250, 436, 520, 658, 855
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 3 cut(s) 256, 306, 553
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 3 cut(s) 253, 303, 550
BstMWI GCNNNNNNNGC 4 cut(s) 185, 367, 376, 715
BstNI CCWGG 2 cut(s) 139, 701
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 3 cut(s) 137, 699, 737
BstSNI TACGTA 1 cut(s) 694
BstV1I GCAGC 5 cut(s) 70, 444, 481, 718, 826
BstV2I GAAGAC 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 6 cut(s) 77, 250, 436, 520, 658, 855
BtsIMutI CAGTG 2 cut(s) 343, 636
Cac8I GCNNGC 2 cut(s) 190, 575
CfoI GCGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 808
CseI GACGC 3 cut(s) 185, 194, 206
Csp6I GTAC 4 cut(s) 280, 580, 641, 691
CviAII CATG 8 cut(s) 169, 193, 217, 362, 574, 614, 812, 847
CviQI GTAC 4 cut(s) 280, 580, 641, 691
DpnI GATC 3 cut(s) 255, 305, 552
DpnII GATC 3 cut(s) 253, 303, 550
Eam1104I CTCTTC 2 cut(s) 103, 638
EarI CTCTTC 2 cut(s) 103, 638
EciI GGCGGA 2 cut(s) 106, 124
Eco105I TACGTA 1 cut(s) 694
Eco130I CCWWGG 3 cut(s) 168, 393, 764
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 3 cut(s) 359, 555, 773
EcoRII CCWGG 2 cut(s) 137, 699
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 3 cut(s) 168, 393, 764
ErhI CCWWGG 3 cut(s) 168, 393, 764
FaeI CATG 8 cut(s) 172, 196, 220, 365, 577, 617, 815, 850
FaiI YATR 8 cut(s) 170, 194, 218, 363, 575, 615, 813, 848
FaqI GGGAC 1 cut(s) 821
FatI CATG 8 cut(s) 168, 192, 216, 361, 573, 613, 811, 846
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 6 cut(s) 84, 211, 433, 495, 707, 840
FokI GGATG 6 cut(s) 64, 257, 423, 527, 645, 842
Fsp4HI GCNGC 6 cut(s) 84, 211, 433, 495, 707, 840
GlaI GCGC 1 cut(s) 209
GluI GCNGC 6 cut(s) 84, 211, 433, 495, 707, 840
HaeII RGCGCY 1 cut(s) 211
HapII CCGG 2 cut(s) 189, 738
HgaI GACGC 3 cut(s) 185, 194, 206
HhaI GCGC 1 cut(s) 210
Hin1I GRCGYC 2 cut(s) 186, 198
Hin1II CATG 8 cut(s) 172, 196, 220, 365, 577, 617, 815, 850
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 5 cut(s) 4, 88, 93, 308, 452
HpaII CCGG 2 cut(s) 189, 738
Hpy166II GTNNAC 4 cut(s) 417, 697, 784, 867
Hpy188I TCNGA 5 cut(s) 313, 457, 595, 663, 753
Hpy188III TCNNGA 3 cut(s) 182, 269, 449
Hpy8I GTNNAC 4 cut(s) 417, 697, 784, 867
Hpy99I CGWCG 2 cut(s) 179, 188
HpyAV CCTTC 8 cut(s) 32, 52, 112, 130, 600, 638, 721, 740
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 3 cut(s) 382, 643, 693
HpyCH4V TGCA 3 cut(s) 389, 432, 658
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 367, 376, 715
HpySE526I ACGT 3 cut(s) 382, 643, 693
Hsp92I GRCGYC 2 cut(s) 186, 198
Hsp92II CATG 8 cut(s) 172, 196, 220, 365, 577, 617, 815, 850
HspAI GCGC 1 cut(s) 208
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 3 cut(s) 253, 303, 550
LmnI GCTCC 2 cut(s) 184, 703
Lsp1109I GCAGC 5 cut(s) 70, 444, 481, 718, 826
LweI GCATC 2 cut(s) 667, 724
MaeII ACGT 3 cut(s) 382, 643, 693
MaeIII GTNAC 3 cut(s) 266, 287, 397
MalI GATC 3 cut(s) 255, 305, 552
MboI GATC 3 cut(s) 253, 303, 550
MboII GAAGA 7 cut(s) 37, 64, 90, 236, 365, 548, 625
MfeI CAATTG 1 cut(s) 283
MluCI AATT 2 cut(s) 283, 316
MlyI GAGTC 2 cut(s) 13, 82
MroNI GCCGGC 1 cut(s) 188
MroXI GAANNNNTTC 2 cut(s) 97, 234
MseI TTAA 3 cut(s) 351, 651, 871
MslI CAYNNNNRTG 1 cut(s) 412
MspA1I CMGCKG 3 cut(s) 83, 442, 709
MspI CCGG 2 cut(s) 189, 738
MspR9I CCNGG 3 cut(s) 139, 701, 739
MunI CAATTG 1 cut(s) 283
MvaI CCWGG 2 cut(s) 139, 701
MwoI GCNNNNNNNGC 4 cut(s) 185, 367, 376, 715
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 739
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 253, 303, 550
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 8 cut(s) 172, 196, 220, 365, 577, 617, 815, 850
NlaIV GGNNCC 2 cut(s) 588, 809
NmuCI GTSAC 2 cut(s) 266, 287
NspI RCATGY 1 cut(s) 577
OliI CACNNNNGTG 1 cut(s) 412
PaeI GCATGC 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 190
PdmI GAANNNNTTC 2 cut(s) 97, 234
PfeI GAWTC 3 cut(s) 93, 308, 452
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 6 cut(s) 85, 212, 434, 496, 708, 841
PleI GAGTC 2 cut(s) 12, 82
PpsI GAGTC 2 cut(s) 12, 82
Ppu21I YACGTR 2 cut(s) 644, 694
Psp1406I AACGTT 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 137, 699
PspGI CCWGG 2 cut(s) 137, 699
PspN4I GGNNCC 2 cut(s) 588, 809
PspPI GGNCC 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 4 cut(s) 281, 581, 642, 692
RsaNI GTAC 4 cut(s) 280, 580, 641, 691
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 3 cut(s) 351, 651, 871
SatI GCNGC 6 cut(s) 84, 211, 433, 495, 707, 840
Sau3AI GATC 3 cut(s) 253, 303, 550
Sau96I GGNCC 1 cut(s) 808
SchI GAGTC 2 cut(s) 13, 82
ScrFI CCNGG 3 cut(s) 139, 701, 739
SfaNI GCATC 2 cut(s) 667, 724
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 412
SnaBI TACGTA 1 cut(s) 694
SphI GCATGC 1 cut(s) 577
Sse9I AATT 2 cut(s) 283, 316
SsiI CCGC 5 cut(s) 81, 117, 135, 211, 440
StyD4I CCNGG 3 cut(s) 137, 699, 737
StyI CCWWGG 3 cut(s) 168, 393, 764
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 3 cut(s) 385, 646, 696
TaqI TCGA 2 cut(s) 91, 306
TasI AATT 2 cut(s) 283, 316
TatI WGTACW 2 cut(s) 279, 579
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 3 cut(s) 93, 308, 452
Tru1I TTAA 3 cut(s) 351, 651, 871
Tru9I TTAA 3 cut(s) 351, 651, 871
TscAI CASTG 2 cut(s) 343, 643
TseFI GTSAC 2 cut(s) 266, 287
TseI GCWGC 5 cut(s) 83, 432, 494, 706, 839
Tsp45I GTSAC 2 cut(s) 266, 287
TspDTI ATGAA 3 cut(s) 531, 630, 863
TspRI CASTG 2 cut(s) 343, 643
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 577
XmnI GAANNNNTTC 2 cut(s) 97, 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.