MD12G1086800.v1.1

Isopentenyl-diphosphate Delta-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009079
Physical Location & Seq
Forward (+)
10648966 .. 10651756
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1086800.v1.1.491

Sequence Viewer

Length: 867 bp
ATGAGCCTCTCCACTGCTCGCCTTCTCCACACTCTCTCCTTCCTTCCCAAATCTCATCCTCCTCTCTTCTCATCCCCTGCCACTCCAACCTTTCTCTTCTCCAAGCCCTCCGCCTTCCCCTCCCTCTCCGCCAGGCTGTCTCTGTCTTTTCACAGTTCCACCATGGGCGACGCTCCTGACGCCGGCATGGACGCCGTCCAGCGACGCCTCATGTTCGAAGACGAATGTATTCTGGTGGATGAGAATGATCGTGTTGTTGGTCACGATACCAAGTACAATTGTCACTTGATGGAAAAGATCGAATCTGAAAATTTGCTTCACAGAGCTTTCAGTGTCTTTTTGTTTAACTCAAAGCATGAGTTGCTTCTTCAGCAACGTTCTGCAACCAAGGTAACATTCCCACTTGTGTGGACAAACACCTGTTGCAGTCATCCACTATACCGTGAATCTGAGCTTATTGATGAGAACTCTCTTGGGGTACGAAATGCTGCTCAAAGGAAGCTTTTGGATGAACTCGGTATTCCTGCTGAAGATGTGCCTGTTGATCAGTTTATCCCGTTGGGTCGCATGCTGTACAAGGCACCTTCTGATGGCAAGTGGGGAGAACATGAACTTGATTACCTCCTCTTCACTGTCCGAGATGTTAATGTGCATCCGAACCCTGATGAGGTAGCTGATATCAAGTACGTAAACCAGGAGGAGCTGAAGGAGCTGTTGAGAAAGGCAGATGCCGGGGAGGAAGGTCTGAAGCTGTCACCTTGGTTCAGACTCGTCGTGGACAACTTCTTGTTCAAGTGGTGGGACCATGTTGAGAAAAACACCATCAAGGAAGCTGCTGACATGAAAACCATCCACAGGTTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
NUDIX

Protein Analysis

289

Amino Acids

32.92

Weight (kDa)

5.64

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 106 - 241 7.8e-15 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 580
AciI CCGC 2 cut(s) 111, 129
AclI AACGTT 1 cut(s) 376
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 4 cut(s) 353, 549, 725, 767
AcyI GRCGYC 3 cut(s) 180, 192, 205
AfaI GTAC 4 cut(s) 275, 480, 575, 686
AfiI CCNNNNNNNGG 4 cut(s) 182, 590, 667, 855
AgsI TTSAA 1 cut(s) 793
AjnI CCWGG 2 cut(s) 131, 693
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AleI CACNNNNGTG 1 cut(s) 406
AluBI AGCT 8 cut(s) 326, 454, 502, 674, 703, 712, 751, 833
AluI AGCT 8 cut(s) 326, 454, 502, 674, 703, 712, 751, 833
Alw26I GTCTC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 488, 833
ApoI RAATTY 1 cut(s) 310
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 802
AsuC2I CCSGG 1 cut(s) 733
AsuHPI GGTGA 1 cut(s) 747
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 802
BanI GGYRCC 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 475, 820
BccI CCATC 4 cut(s) 283, 584, 830, 857
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 133, 695
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 733
BcoDI GTCTC 1 cut(s) 144
BisI GCNGC 2 cut(s) 489, 834
BlsI GCNGC 2 cut(s) 490, 835
Bme1390I CCNGG 3 cut(s) 133, 695, 733
Bme18I GGWCC 1 cut(s) 802
BmgT120I GGNCC 1 cut(s) 802
BmiI GGNNCC 2 cut(s) 582, 803
BmrFI CCNGG 3 cut(s) 133, 695, 733
BmsI GCATC 2 cut(s) 661, 718
BpiI GAAGAC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 733
BsaAI YACGTR 1 cut(s) 688
BsaHI GRCGYC 3 cut(s) 180, 192, 205
BsaJI CCNNGG 4 cut(s) 162, 387, 732, 758
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 590, 667, 855
Bse118I RCCGGY 1 cut(s) 182
BseBI CCWGG 2 cut(s) 133, 695
BseDI CCNNGG 4 cut(s) 162, 387, 732, 758
BseGI GGATG 7 cut(s) 55, 71, 244, 430, 514, 652, 849
BseLI CCNNNNNNNGG 4 cut(s) 182, 590, 667, 855
BseMII CTCAG 1 cut(s) 441
BseRI GAGGAG 3 cut(s) 51, 614, 713
BseXI GCAGC 2 cut(s) 475, 820
BshNI GGYRCC 1 cut(s) 580
BsiSI CCGG 2 cut(s) 183, 732
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 4 cut(s) 182, 590, 667, 855
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 216
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 247, 297, 544
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 2 cut(s) 111, 129
BspCNI CTCAG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 582, 803
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 162, 387, 732, 758
BssMI GATC 3 cut(s) 247, 297, 544
BssNI GRCGYC 3 cut(s) 180, 192, 205
BssT1I CCWWGG 3 cut(s) 162, 387, 758
Bst2UI CCWGG 2 cut(s) 133, 695
Bst4CI ACNGT 3 cut(s) 155, 443, 634
Bst6I CTCTTC 3 cut(s) 71, 101, 632
BstACI GRCGYC 3 cut(s) 180, 192, 205
BstAPI GCANNNNNTGC 1 cut(s) 361
BstAUI TGTACA 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 19, 184, 569
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 7 cut(s) 55, 71, 244, 430, 514, 652, 849
BstKTI GATC 3 cut(s) 250, 300, 547
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 3 cut(s) 247, 297, 544
BstMWI GCNNNNNNNGC 4 cut(s) 179, 361, 370, 709
BstNI CCWGG 2 cut(s) 133, 695
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 3 cut(s) 131, 693, 731
BstSNI TACGTA 1 cut(s) 688
BstV1I GCAGC 2 cut(s) 475, 820
BstV2I GAAGAC 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 7 cut(s) 55, 71, 244, 430, 514, 652, 849
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 337, 630
Cac8I GCNNGC 3 cut(s) 19, 184, 569
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 802
CseI GACGC 4 cut(s) 179, 188, 200, 213
Csp6I GTAC 4 cut(s) 274, 479, 574, 685
CviAII CATG 8 cut(s) 163, 187, 211, 356, 568, 608, 806, 841
CviQI GTAC 4 cut(s) 274, 479, 574, 685
DdeI CTNAG 1 cut(s) 450
DpnI GATC 3 cut(s) 249, 299, 546
DpnII GATC 3 cut(s) 247, 297, 544
Eam1104I CTCTTC 3 cut(s) 71, 101, 632
EarI CTCTTC 3 cut(s) 71, 101, 632
EciI GGCGGA 2 cut(s) 100, 118
Eco105I TACGTA 1 cut(s) 688
Eco130I CCWWGG 3 cut(s) 162, 387, 758
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 802
Eco57I CTGAAG 4 cut(s) 353, 549, 725, 767
EcoRII CCWGG 2 cut(s) 131, 693
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 3 cut(s) 162, 387, 758
ErhI CCWWGG 3 cut(s) 162, 387, 758
FaeI CATG 8 cut(s) 166, 190, 214, 359, 571, 611, 809, 844
FaiI YATR 9 cut(s) 164, 188, 212, 357, 439, 569, 609, 807, 842
FaqI GGGAC 1 cut(s) 815
FatI CATG 8 cut(s) 162, 186, 210, 355, 567, 607, 805, 840
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 489, 834
FokI GGATG 7 cut(s) 42, 58, 251, 417, 521, 639, 836
Fsp4HI GCNGC 2 cut(s) 489, 834
GluI GCNGC 2 cut(s) 489, 834
HapII CCGG 2 cut(s) 183, 732
HgaI GACGC 4 cut(s) 179, 188, 200, 213
Hin1I GRCGYC 3 cut(s) 180, 192, 205
Hin1II CATG 8 cut(s) 166, 190, 214, 359, 571, 611, 809, 844
HincII GTYRAC 1 cut(s) 861
HindII GTYRAC 1 cut(s) 861
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 3 cut(s) 302, 446, 768
HpaII CCGG 2 cut(s) 183, 732
HphI GGTGA 1 cut(s) 747
Hpy166II GTNNAC 4 cut(s) 411, 691, 778, 861
Hpy188I TCNGA 7 cut(s) 307, 451, 589, 638, 657, 747, 767
Hpy188III TCNNGA 2 cut(s) 176, 263
Hpy8I GTNNAC 4 cut(s) 411, 691, 778, 861
Hpy99I CGWCG 3 cut(s) 173, 207, 776
HpyAV CCTTC 7 cut(s) 32, 49, 53, 124, 594, 700, 734
HpyCH4III ACNGT 3 cut(s) 155, 443, 634
HpyCH4IV ACGT 2 cut(s) 376, 687
HpyCH4V TGCA 3 cut(s) 383, 426, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 361, 370, 709
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 376, 687
Hsp92I GRCGYC 3 cut(s) 180, 192, 205
Hsp92II CATG 8 cut(s) 166, 190, 214, 359, 571, 611, 809, 844
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 3 cut(s) 247, 297, 544
LmnI GCTCC 3 cut(s) 178, 700, 709
Lsp1109I GCAGC 2 cut(s) 475, 820
LweI GCATC 2 cut(s) 661, 718
MaeII ACGT 2 cut(s) 376, 687
MaeIII GTNAC 4 cut(s) 260, 281, 391, 753
MalI GATC 3 cut(s) 249, 299, 546
MboI GATC 3 cut(s) 247, 297, 544
MboII GAAGA 6 cut(s) 58, 88, 230, 359, 542, 619
MfeI CAATTG 1 cut(s) 277
MluCI AATT 2 cut(s) 277, 310
MlyI GAGTC 1 cut(s) 762
MmeI TCCRAC 1 cut(s) 110
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 3 cut(s) 345, 645, 865
MslI CAYNNNNRTG 1 cut(s) 406
MspI CCGG 2 cut(s) 183, 732
MspR9I CCNGG 3 cut(s) 133, 695, 733
MunI CAATTG 1 cut(s) 277
MvaI CCWGG 2 cut(s) 133, 695
MwoI GCNNNNNNNGC 4 cut(s) 179, 361, 370, 709
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 1 cut(s) 733
NcoI CCATGG 1 cut(s) 162
NdeII GATC 3 cut(s) 247, 297, 544
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 8 cut(s) 166, 190, 214, 359, 571, 611, 809, 844
NlaIV GGNNCC 2 cut(s) 582, 803
NmuCI GTSAC 3 cut(s) 260, 281, 753
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 406
PaeI GCATGC 1 cut(s) 571
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 302, 446
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 2 cut(s) 490, 835
PleI GAGTC 1 cut(s) 762
PpsI GAGTC 1 cut(s) 762
Ppu21I YACGTR 1 cut(s) 688
Psp1406I AACGTT 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 131, 693
PspGI CCWGG 2 cut(s) 131, 693
PspN4I GGNNCC 2 cut(s) 582, 803
PspPI GGNCC 1 cut(s) 802
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 4 cut(s) 275, 480, 575, 686
RsaNI GTAC 4 cut(s) 274, 479, 574, 685
RseI CAYNNNNRTG 1 cut(s) 406
SaqAI TTAA 3 cut(s) 345, 645, 865
SatI GCNGC 2 cut(s) 489, 834
Sau3AI GATC 3 cut(s) 247, 297, 544
Sau96I GGNCC 1 cut(s) 802
SchI GAGTC 1 cut(s) 762
ScrFI CCNGG 3 cut(s) 133, 695, 733
SfaNI GCATC 2 cut(s) 661, 718
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 406
SnaBI TACGTA 1 cut(s) 688
SphI GCATGC 1 cut(s) 571
Sse9I AATT 2 cut(s) 277, 310
SsiI CCGC 2 cut(s) 111, 129
StyD4I CCNGG 3 cut(s) 131, 693, 731
StyI CCWWGG 3 cut(s) 162, 387, 758
TaaI ACNGT 3 cut(s) 155, 443, 634
TaiI ACGT 2 cut(s) 379, 690
TaqI TCGA 2 cut(s) 216, 300
TasI AATT 2 cut(s) 277, 310
TatI WGTACW 2 cut(s) 273, 573
TfiI GAWTC 2 cut(s) 302, 446
Tru1I TTAA 3 cut(s) 345, 645, 865
Tru9I TTAA 3 cut(s) 345, 645, 865
TscAI CASTG 3 cut(s) 19, 337, 637
TseFI GTSAC 3 cut(s) 260, 281, 753
TseI GCWGC 2 cut(s) 488, 833
Tsp45I GTSAC 3 cut(s) 260, 281, 753
TspDTI ATGAA 3 cut(s) 525, 624, 857
TspRI CASTG 3 cut(s) 19, 337, 637
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.