Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009005

Physical Location & Seq

Genomic Coordinates Reverse (-)

32270767 .. 32278446

Gene Structure 7680 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1248300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGGATTCGGACGAAGAGTTCGACATGCTCGACGCCAACGATGACGACGACGATTTCTACAGTGGCGGCGATGATGATGCAGCTGCCGCTATGGACAGCGACGACGCTGGAGTTGCAGATTACGAGTTCATCGACAACGACTCCGACGATTCCAATGATGTCACCTCGCATCGTTACCAGCAAAACTACAATGTTTTGACAGAAGCTGACATTCGTCAGCATCAAGAGGAGGATATTATGAGGATATCTACAGTGCTCTCTATTCCAAAATTTGCAGCTAGTATACTGCTCCGCTACTATAATTGGAGTGTCAGTAAAGTACACGATGAGTGGTTTGCAGATGAAGAAAGGGTTCGCAGGGCTGTTGGCTTGCCAGAGAAACCAGTTGTTGAATATCCTAATGCTAGTGAACTTCATTGTGGGATTTGTTTTGAGATCTATCCTCGTGATAAGATGCATGCTGCAGCTTGTGGTCATCCTTTCTGTTCCTCATGCTGGGCAGGCTATATTAGCACAGCCATTAATGATGGCCCTGGATGTTTGATGTTGCGCTGTCCGGATCCATCTTGTAGTGCCGTTGTTTGCCAAGATATGATTAATGATTTGGCTTCCTATGAAGAGAAGGAGAAATATTCTCGCTACTTTATTAGATCTTATGTTGAAGACAATAGGAAGACAAAATGGTGTCCTGCTCCAGGCTGTGATTATGCTATAGATTTTATTGTTGGTGGTGGAAGCTATGATGTTACCTGCCGCTGTTCATATAGCTTTTGCTGGAACTGTACTGAGGAAGCTCACCGTCCAGTTGATTGTGGCACTGTGTCAAAATGGATAATGAAGAATAGTGCAGAATCTGAAAATATGAATTGGATATTGGCTAATTCCAAGCCCTGCCCGAAGTGCAAACGGCCGATTGAGAAAAACCAAGGCTGTATGCATATCACTTGCACACCACCTTGTAAATTTGAGTTTTGCTGGCTTTGCCTCGGTGCGTGGTCGGATCATGGTGAAAGGACGGGTGGCTTTTATGCATGTAACCGTTATGAGACAGCAAAGCAGGAGGGAGTGTATGATGAGTCAGAGAAGCGAAGAGAGATGGCCAAGAACTCTCTGGAGAGGTACACTCATTATTATGAAAGATGGGCAACCAATCAATCGTCCCGGCAGAAAGCACTTGCAGACTTGCAGCAGATGCAAACTGTGCACCTTGAGAAGCTGAGCGATACCCAGTGCCAACCTGAGTCACAGCTGAAGTTCATTACAGAGGCCTGGCTACAGATAGTTGAATGTAGGCGAGTACTAAAATGGACATATGCTTATGGATATTATTTACCGGAGCATGAACATGCCAAGAGACAGTTCTTTGAGTACTTGCAAGGTGAAGCTGAGTCTGGGTTGGAACGGCTTCATCAATGTGCAGAAAAGGAGCTACAAGTTTACCTCAATGATGAGGACCCATCAAAAGATTTCAATGAGTTCCGCACAAAACTTGCCGGATTGACCAGTGTGACTCGAAACTACTTTGAAAATTTAGTTCGAGCTCTGGAGAATGGTCTATCAGACGTGGATTCCCATGGTGCCTGCAGCAGGACAGTAAGCTCAAAGAGTTTGGGAGGTTCAAGTAAGGGAAGAGGTGGGAGGGGCAAGGCTGCAACATCCAGGTCAAGTAGTTCCAGTAGAAACATTGACGATTTAGGCCACTGGTCCTGTGAACATTGCACCTTTGCAAATGTCAGGCCGGCCAGTGTGTGCCAGATGTGTCAACAGCGCCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

IBR zf-C3HC4

Protein Analysis

592

Amino Acids

67.44

Weight (kDa)

5.09

Isoelectric Point (pI)

54.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_ARI1	PF21235	73 - 112	4.6e-09	E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR	PF01485	209 - 271	2e-16	IBR domain, a half RING-finger domain
IBR	PF01485	288 - 335	5.4e-07	IBR domain, a half RING-finger domain
IBR_1	PF22191	293 - 333	2.7e-14	IBR domain
IBR_2	PF22605	294 - 333	4.4e-12	IBR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65430
fragaria_vesca	FvH4_6g00130 FvH4_6g00130
malus_domestica	MD04G1248300.v1.1 MD12G1262400.v1.1
prunus_persica	Prupe.6G364600_v2.0.a1
pyrus_communis	pycom04g21900 pycom12g24160
rosa_chinensis	RchiOBHm_Chr2g0127711 RchiOBHm_Chr3g0447471 RchiOBHm_Chr6g0267381
rosa_laevigata	RLG00000025995
rosa_multiflora	Rmu_sc0006572.1_g000005
rosa_roxburghii	Rroxscaffold_5G00344110 Rroxscaffold_6G00425690
rosa_rugosa	Rorug02G0602100
rosa_samantha	Rh2BG278300 Rh3AG002300 Rh3BG001800 Rh3CG002100 Rh3DG002200
rosa_wichuraiana	Rw3G000120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	758
AccB1I	GGYRCC	1 cut(s)	1577
AccI	GTMKAC	1 cut(s)	283
AccIII	TCCGGA	1 cut(s)	556
AciI	CCGC	5 cut(s)	66, 87, 292, 754, 1480
AclWI	GGATC	3 cut(s)	554, 567, 1008
AcoI	YGGCCR	3 cut(s)	908, 1098, 1740
AcsI	RAATTY	3 cut(s)	269, 962, 1528
AcuI	CTGAAG	1 cut(s)	1271
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	5 cut(s)	321, 784, 1121, 1299, 1370
AfiI	CCNNNNNNNGG	3 cut(s)	495, 695, 1587
AgsI	TTSAA	6 cut(s)	392, 662, 1286, 1471, 1526, 1620
AjiI	CACGTC	1 cut(s)	1564
AjnI	CCWGG	4 cut(s)	532, 694, 1268, 1658
AjuI	GAANNNNNNNTTGG	4 cut(s)	857, 889, 1343, 1375
AloI	GAACNNNNNNTCC	1 cut(s)	34
Alw21I	GWGCWC	3 cut(s)	258, 1206, 1543
Alw26I	GTCTC	2 cut(s)	1040, 1348
Alw44I	GTGCAC	1 cut(s)	1202
AlwI	GGATC	3 cut(s)	554, 567, 1008
AlwNI	CAGNNNCTG	2 cut(s)	206, 854
Aor13HI	TCCGGA	1 cut(s)	556
AoxI	GGCC	7 cut(s)	529, 908, 1098, 1266, 1696, 1736, 1740
ApaLI	GTGCAC	1 cut(s)	1202
ApeKI	GCWGC	8 cut(s)	80, 83, 275, 461, 464, 1186, 1584, 1649
ApoI	RAATTY	3 cut(s)	269, 962, 1528
ArsI	GACNNNNNNTTYG	1 cut(s)	34
AseI	ATTAAT	2 cut(s)	522, 597
Asp700I	GAANNNNTTC	2 cut(s)	351, 1404
AspLEI	GCGC	2 cut(s)	552, 1770
AspS9I	GGNCC	3 cut(s)	530, 1453, 1704
AsuC2I	CCSGG	1 cut(s)	1162
AsuHPI	GGTGA	4 cut(s)	154, 788, 1019, 1391
AvaII	GGWCC	2 cut(s)	1453, 1704
BaeGI	GKGCMC	1 cut(s)	1206
BalI	TGGCCA	1 cut(s)	1100
BamHI	GGATCC	1 cut(s)	559
BanI	GGYRCC	1 cut(s)	1577
BanII	GRGCYC	1 cut(s)	1543
BauI	CACGAG	1 cut(s)	444
BbsI	GAAGAC	2 cut(s)	669, 680
Bbv12I	GWGCWC	3 cut(s)	258, 1206, 1543
BbvI	GCAGC	8 cut(s)	70, 92, 287, 448, 476, 1198, 1596, 1636
BccI	CCATC	5 cut(s)	521, 571, 1090, 1134, 1465
BceAI	ACGGC	3 cut(s)	560, 923, 1418
BcgI	CGANNNNNNTGC	2 cut(s)	59, 93
BciT130I	CCWGG	4 cut(s)	534, 696, 1270, 1660
BcnI	CCSGG	1 cut(s)	1162
BcoDI	GTCTC	2 cut(s)	1040, 1348
BfaI	CTAG	2 cut(s)	279, 405
BfmI	CTRYAG	6 cut(s)	58, 249, 462, 711, 1274, 1582
BfoI	RGCGCY	1 cut(s)	1771
BfuAI	ACCTGC	1 cut(s)	758
BglII	AGATCT	2 cut(s)	435, 650
BlpI	GCTNAGC	1 cut(s)	1217
BmcAI	AGTACT	2 cut(s)	1299, 1370
Bme1390I	CCNGG	5 cut(s)	534, 696, 1162, 1270, 1660
Bme18I	GGWCC	2 cut(s)	1453, 1704
BmgBI	CACGTC	1 cut(s)	1564
BmgT120I	GGNCC	3 cut(s)	530, 1453, 1704
BmiI	GGNNCC	3 cut(s)	561, 1455, 1579
BmrFI	CCNGG	5 cut(s)	534, 696, 1162, 1270, 1660
BmrI	ACTGGG	1 cut(s)	1222
BmsI	GCATC	5 cut(s)	67, 178, 229, 444, 1182
BmuI	ACTGGG	1 cut(s)	1222
BoxI	GACNNNNGTC	1 cut(s)	213
BpiI	GAAGAC	2 cut(s)	669, 680
BplI	GAGNNNNNCTC	2 cut(s)	1108, 1140
BpmI	CTGGAG	4 cut(s)	129, 678, 1133, 1565
Bpu1102I	GCTNAGC	1 cut(s)	1217
BpuEI	CTTGAG	1 cut(s)	1229
BpuMI	CCSGG	1 cut(s)	1162
BsaHI	GRCGYC	1 cut(s)	33
BsaJI	CCNNGG	4 cut(s)	532, 925, 985, 1573
BsaWI	WCCGGW	2 cut(s)	556, 1333
BsaXI	ACNNNNNCTCC	6 cut(s)	125, 155, 298, 328, 1052, 1082
Bsc4I	CCNNNNNNNGG	3 cut(s)	495, 695, 1587
Bse118I	RCCGGY	2 cut(s)	1738, 1770
Bse1I	ACTGG	7 cut(s)	383, 803, 1228, 1503, 1674, 1706, 1743
Bse3DI	GCAATG	1 cut(s)	1714
BseAI	TCCGGA	1 cut(s)	556
BseBI	CCWGG	4 cut(s)	534, 696, 1270, 1660
BseDI	CCNNGG	4 cut(s)	532, 925, 985, 1573
BseGI	GGATG	3 cut(s)	475, 542, 1655
BseLI	CCNNNNNNNGG	3 cut(s)	495, 695, 1587
BseMI	GCAATG	1 cut(s)	1714
BseMII	CTCAG	4 cut(s)	777, 1208, 1230, 1377
BseNI	ACTGG	7 cut(s)	383, 803, 1228, 1503, 1674, 1706, 1743
BseRI	GAGGAG	1 cut(s)	242
BseSI	GKGCMC	1 cut(s)	1206
BseX3I	CGGCCG	1 cut(s)	908
BseXI	GCAGC	8 cut(s)	70, 92, 287, 448, 476, 1198, 1596, 1636
BseYI	CCCAGC	1 cut(s)	495
BsgI	GTGCAG	2 cut(s)	867, 1437
Bsh1285I	CGRYCG	1 cut(s)	911
BshFI	GGCC	7 cut(s)	531, 910, 1100, 1268, 1698, 1738, 1742
BshNI	GGYRCC	1 cut(s)	1577
BsiEI	CGRYCG	1 cut(s)	911
BsiHKAI	GWGCWC	3 cut(s)	258, 1206, 1543
BsiSI	CCGG	6 cut(s)	557, 1162, 1334, 1494, 1739, 1771
BslFI	GGGAC	1 cut(s)	1144
BslI	CCNNNNNNNGG	3 cut(s)	495, 695, 1587
BsmAI	GTCTC	2 cut(s)	1040, 1348
BsmFI	GGGAC	1 cut(s)	1144
BsnI	GGCC	7 cut(s)	531, 910, 1100, 1268, 1698, 1738, 1742
Bsp1286I	GDGCHC	3 cut(s)	258, 1206, 1543
Bsp13I	TCCGGA	1 cut(s)	556
Bsp143I	GATC	4 cut(s)	435, 559, 650, 1000
Bsp1720I	GCTNAGC	1 cut(s)	1217
Bsp19I	CCATGG	1 cut(s)	1573
BspACI	CCGC	5 cut(s)	66, 87, 292, 754, 1480
BspANI	GGCC	7 cut(s)	531, 910, 1100, 1268, 1698, 1738, 1742
BspCNI	CTCAG	4 cut(s)	778, 1209, 1231, 1378
BspEI	TCCGGA	1 cut(s)	556
BspLI	GGNNCC	3 cut(s)	561, 1455, 1579
BspMAI	CTGCAG	2 cut(s)	466, 1586
BspMI	ACCTGC	1 cut(s)	758
BspPI	GGATC	3 cut(s)	554, 567, 1008
BspT107I	GGYRCC	1 cut(s)	1577
BsrDI	GCAATG	1 cut(s)	1714
BsrFI	RCCGGY	2 cut(s)	1738, 1770
BsrI	ACTGG	7 cut(s)	383, 803, 1228, 1503, 1674, 1706, 1743
BssAI	RCCGGY	2 cut(s)	1738, 1770
BssECI	CCNNGG	4 cut(s)	532, 925, 985, 1573
BssMI	GATC	4 cut(s)	435, 559, 650, 1000
BssNAI	GTATAC	1 cut(s)	284
BssNI	GRCGYC	1 cut(s)	33
BssSI	CACGAG	1 cut(s)	444
BssT1I	CCWWGG	2 cut(s)	925, 1573
Bst1107I	GTATAC	1 cut(s)	284
Bst2BI	CACGAG	1 cut(s)	444
Bst2UI	CCWGG	4 cut(s)	534, 696, 1270, 1660
Bst4CI	ACNGT	9 cut(s)	62, 253, 782, 800, 820, 1040, 1201, 1359, 1594
Bst6I	CTCTTC	4 cut(s)	9, 612, 1084, 1624
BstACI	GRCGYC	1 cut(s)	33
BstAPI	GCANNNNNTGC	2 cut(s)	1192, 1201
BstC8I	GCNNGC	6 cut(s)	371, 459, 502, 977, 1582, 1740
BstDEI	CTNAG	4 cut(s)	786, 1217, 1239, 1386
BstDSI	CCRYGG	1 cut(s)	1573
BstENI	CCTNNNNNAGG	2 cut(s)	693, 1585
BstF5I	GGATG	3 cut(s)	475, 542, 1655
BstH2I	RGCGCY	1 cut(s)	1771
BstHHI	GCGC	2 cut(s)	552, 1770
BstKTI	GATC	4 cut(s)	438, 562, 653, 1003
BstMAI	GTCTC	2 cut(s)	1040, 1348
BstMBI	GATC	4 cut(s)	435, 559, 650, 1000
BstMCI	CGRYCG	1 cut(s)	911
BstMWI	GCNNNNNNNGC	8 cut(s)	86, 113, 501, 510, 900, 981, 1192, 1201
BstNI	CCWGG	4 cut(s)	534, 696, 1270, 1660
BstNSI	RCATGY	4 cut(s)	28, 461, 1035, 1349
BstPAI	GACNNNNGTC	1 cut(s)	213
BstSCI	CCNGG	5 cut(s)	532, 694, 1160, 1268, 1658
BstSFI	CTRYAG	6 cut(s)	58, 249, 462, 711, 1274, 1582
BstSLI	GKGCMC	1 cut(s)	1206
BstV1I	GCAGC	8 cut(s)	70, 92, 287, 448, 476, 1198, 1596, 1636
BstV2I	GAAGAC	2 cut(s)	669, 680
BstX2I	RGATCY	3 cut(s)	435, 559, 650
BstYI	RGATCY	3 cut(s)	435, 559, 650
BstZ17I	GTATAC	1 cut(s)	284
BstZI	CGGCCG	1 cut(s)	908
BsuRI	GGCC	7 cut(s)	531, 910, 1100, 1268, 1698, 1738, 1742
BtgI	CCRYGG	1 cut(s)	1573
BtgZI	GCGATG	1 cut(s)	84
BtrI	CACGTC	1 cut(s)	1564
BtsCI	GGATG	3 cut(s)	475, 542, 1655
BtsIMutI	CAGTG	7 cut(s)	67, 258, 816, 1235, 1510, 1699, 1750
BveI	ACCTGC	1 cut(s)	758
Cac8I	GCNNGC	6 cut(s)	371, 459, 502, 977, 1582, 1740
CaiI	CAGNNNCTG	2 cut(s)	206, 854
CfoI	GCGC	2 cut(s)	552, 1770
Cfr10I	RCCGGY	2 cut(s)	1738, 1770
Cfr13I	GGNCC	3 cut(s)	530, 1453, 1704
CseI	GACGC	2 cut(s)	41, 113
Csp6I	GTAC	5 cut(s)	320, 783, 1120, 1298, 1369
CviAII	CATG	8 cut(s)	25, 458, 492, 1004, 1032, 1340, 1346, 1574
CviQI	GTAC	5 cut(s)	320, 783, 1120, 1298, 1369
DdeI	CTNAG	4 cut(s)	786, 1217, 1239, 1386
DpnI	GATC	4 cut(s)	437, 561, 652, 1002
DpnII	GATC	4 cut(s)	435, 559, 650, 1000
EaeI	YGGCCR	3 cut(s)	908, 1098, 1740
EagI	CGGCCG	1 cut(s)	908
Eam1104I	CTCTTC	4 cut(s)	9, 612, 1084, 1624
EarI	CTCTTC	4 cut(s)	9, 612, 1084, 1624
Ecl136II	GAGCTC	1 cut(s)	1541
EclXI	CGGCCG	1 cut(s)	908
Eco130I	CCWWGG	2 cut(s)	925, 1573
Eco147I	AGGCCT	1 cut(s)	1268
Eco24I	GRGCYC	1 cut(s)	1543
Eco32I	GATATC	1 cut(s)	246
Eco47I	GGWCC	2 cut(s)	1453, 1704
Eco52I	CGGCCG	1 cut(s)	908
Eco53kI	GAGCTC	1 cut(s)	1541
Eco57I	CTGAAG	1 cut(s)	1271
EcoICRI	GAGCTC	1 cut(s)	1541
EcoNI	CCTNNNNNAGG	2 cut(s)	693, 1585
EcoO109I	RGGNCCY	1 cut(s)	1453
EcoRII	CCWGG	4 cut(s)	532, 694, 1268, 1658
EcoRV	GATATC	1 cut(s)	246
EcoT14I	CCWWGG	2 cut(s)	925, 1573
EcoT22I	ATGCAT	3 cut(s)	459, 939, 1033
EcoT38I	GRGCYC	1 cut(s)	1543
ErhI	CCWWGG	2 cut(s)	925, 1573
FaeI	CATG	8 cut(s)	28, 461, 495, 1007, 1035, 1343, 1349, 1577
FaqI	GGGAC	1 cut(s)	1144
FatI	CATG	8 cut(s)	24, 457, 491, 1003, 1031, 1339, 1345, 1573
FauNDI	CATATG	1 cut(s)	1312
FblI	GTMKAC	1 cut(s)	283
FokI	GGATG	3 cut(s)	462, 549, 1642
FriOI	GRGCYC	1 cut(s)	1543
FseI	GGCCGGCC	1 cut(s)	1742
FspBI	CTAG	2 cut(s)	279, 405
GlaI	GCGC	2 cut(s)	551, 1769
GsaI	CCCAGC	1 cut(s)	499
GsuI	CTGGAG	4 cut(s)	129, 678, 1133, 1565
HaeII	RGCGCY	1 cut(s)	1771
HaeIII	GGCC	7 cut(s)	531, 910, 1100, 1268, 1698, 1738, 1742
HapII	CCGG	6 cut(s)	557, 1162, 1334, 1494, 1739, 1771
HgaI	GACGC	2 cut(s)	41, 113
HhaI	GCGC	2 cut(s)	552, 1770
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	8 cut(s)	28, 461, 495, 1007, 1035, 1343, 1349, 1577
Hin6I	GCGC	2 cut(s)	550, 1768
HinP1I	GCGC	2 cut(s)	550, 1768
HincII	GTYRAC	1 cut(s)	1763
HindII	GTYRAC	1 cut(s)	1763
HinfI	GANTC	9 cut(s)	5, 140, 149, 851, 1076, 1241, 1388, 1510, 1568
HpaII	CCGG	6 cut(s)	557, 1162, 1334, 1494, 1739, 1771
HphI	GGTGA	4 cut(s)	154, 788, 1019, 1391
Hpy166II	GTNNAC	8 cut(s)	284, 322, 410, 1122, 1204, 1438, 1712, 1763
Hpy188I	TCNGA	6 cut(s)	10, 145, 856, 1000, 1081, 1561
Hpy188III	TCNNGA	5 cut(s)	224, 446, 557, 1112, 1544
Hpy8I	GTNNAC	8 cut(s)	284, 322, 410, 1122, 1204, 1438, 1712, 1763
Hpy99I	CGWCG	6 cut(s)	35, 50, 53, 104, 107, 149
HpyAV	CCTTC	1 cut(s)	616
HpyCH4III	ACNGT	9 cut(s)	62, 253, 782, 800, 820, 1040, 1201, 1359, 1594
HpyCH4IV	ACGT	1 cut(s)	1563
HpyF10VI	GCNNNNNNNGC	8 cut(s)	86, 113, 501, 510, 900, 981, 1192, 1201
HpyF3I	CTNAG	4 cut(s)	786, 1217, 1239, 1386
HpySE526I	ACGT	1 cut(s)	1563
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	8 cut(s)	28, 461, 495, 1007, 1035, 1343, 1349, 1577
HspAI	GCGC	2 cut(s)	550, 1768
Kpn2I	TCCGGA	1 cut(s)	556
KroI	GCCGGC	1 cut(s)	1738
KroNI	GCCGGC	1 cut(s)	1740
Kzo9I	GATC	4 cut(s)	435, 559, 650, 1000
LmnI	GCTCC	4 cut(s)	294, 697, 1336, 1426
Lsp1109I	GCAGC	8 cut(s)	70, 92, 287, 448, 476, 1198, 1596, 1636
LweI	GCATC	5 cut(s)	67, 178, 229, 444, 1182
MaeI	CTAG	2 cut(s)	279, 405
MaeII	ACGT	1 cut(s)	1563
MaeIII	GTNAC	6 cut(s)	160, 173, 745, 1034, 1242, 1507
MalI	GATC	4 cut(s)	437, 561, 652, 1002
MboI	GATC	4 cut(s)	435, 559, 650, 1000
MboII	GAAGA	8 cut(s)	26, 356, 629, 674, 685, 850, 1101, 1641
MflI	RGATCY	3 cut(s)	435, 559, 650
MhlI	GDGCHC	3 cut(s)	258, 1206, 1543
MlsI	TGGCCA	1 cut(s)	1100
MluCI	AATT	6 cut(s)	269, 301, 865, 880, 962, 1528
MluNI	TGGCCA	1 cut(s)	1100
MlyI	GAGTC	5 cut(s)	134, 1085, 1250, 1397, 1504
MmeI	TCCRAC	3 cut(s)	168, 978, 1377
Mox20I	TGGCCA	1 cut(s)	1100
Mph1103I	ATGCAT	3 cut(s)	459, 939, 1033
MroI	TCCGGA	1 cut(s)	556
MroNI	GCCGGC	1 cut(s)	1738
MroXI	GAANNNNTTC	2 cut(s)	351, 1404
MscI	TGGCCA	1 cut(s)	1100
MseI	TTAA	2 cut(s)	522, 597
MslI	CAYNNNNRTG	3 cut(s)	1131, 1344, 1413
Msp20I	TGGCCA	1 cut(s)	1100
MspA1I	CMGCKG	3 cut(s)	83, 756, 1249
MspI	CCGG	6 cut(s)	557, 1162, 1334, 1494, 1739, 1771
MspR9I	CCNGG	5 cut(s)	534, 696, 1162, 1270, 1660
MvaI	CCWGG	4 cut(s)	534, 696, 1270, 1660
MwoI	GCNNNNNNNGC	8 cut(s)	86, 113, 501, 510, 900, 981, 1192, 1201
NaeI	GCCGGC	1 cut(s)	1740
NciI	CCSGG	1 cut(s)	1162
NcoI	CCATGG	1 cut(s)	1573
NdeI	CATATG	1 cut(s)	1312
NdeII	GATC	4 cut(s)	435, 559, 650, 1000
NgoMIV	GCCGGC	1 cut(s)	1738
NlaIII	CATG	8 cut(s)	28, 461, 495, 1007, 1035, 1343, 1349, 1577
NlaIV	GGNNCC	3 cut(s)	561, 1455, 1579
NmuCI	GTSAC	3 cut(s)	160, 1242, 1507
NsiI	ATGCAT	3 cut(s)	459, 939, 1033
NspI	RCATGY	4 cut(s)	28, 461, 1035, 1349
PaeI	GCATGC	1 cut(s)	461
PceI	AGGCCT	1 cut(s)	1268
PcsI	WCGNNNNNNNCGW	6 cut(s)	18, 27, 36, 45, 129, 144
PdiI	GCCGGC	1 cut(s)	1740
PdmI	GAANNNNTTC	2 cut(s)	351, 1404
PfeI	GAWTC	4 cut(s)	5, 149, 851, 1568
PleI	GAGTC	5 cut(s)	134, 1084, 1249, 1396, 1504
PpsI	GAGTC	5 cut(s)	134, 1084, 1249, 1396, 1504
PpuMI	RGGWCCY	1 cut(s)	1453
PshAI	GACNNNNGTC	1 cut(s)	213
PshBI	ATTAAT	2 cut(s)	522, 597
Psp124BI	GAGCTC	1 cut(s)	1543
Psp5II	RGGWCCY	1 cut(s)	1453
Psp6I	CCWGG	4 cut(s)	532, 694, 1268, 1658
PspFI	CCCAGC	1 cut(s)	495
PspGI	CCWGG	4 cut(s)	532, 694, 1268, 1658
PspN4I	GGNNCC	3 cut(s)	561, 1455, 1579
PspPI	GGNCC	3 cut(s)	530, 1453, 1704
PspPPI	RGGWCCY	1 cut(s)	1453
PstI	CTGCAG	2 cut(s)	466, 1586
PstNI	CAGNNNCTG	2 cut(s)	206, 854
PsuI	RGATCY	3 cut(s)	435, 559, 650
PvuII	CAGCTG	2 cut(s)	83, 1249
RigI	GGCCGGCC	1 cut(s)	1742
RsaI	GTAC	5 cut(s)	321, 784, 1121, 1299, 1370
RsaNI	GTAC	5 cut(s)	320, 783, 1120, 1298, 1369
RseI	CAYNNNNRTG	3 cut(s)	1131, 1344, 1413
SacI	GAGCTC	1 cut(s)	1543
SaqAI	TTAA	2 cut(s)	522, 597
Sau3AI	GATC	4 cut(s)	435, 559, 650, 1000
Sau96I	GGNCC	3 cut(s)	530, 1453, 1704
ScaI	AGTACT	2 cut(s)	1299, 1370
SchI	GAGTC	5 cut(s)	134, 1085, 1250, 1397, 1504
ScrFI	CCNGG	5 cut(s)	534, 696, 1162, 1270, 1660
SduI	GDGCHC	3 cut(s)	258, 1206, 1543
SfaNI	GCATC	5 cut(s)	67, 178, 229, 444, 1182
SfcI	CTRYAG	6 cut(s)	58, 249, 462, 711, 1274, 1582
SinI	GGWCC	2 cut(s)	1453, 1704
SmiMI	CAYNNNNRTG	3 cut(s)	1131, 1344, 1413
SmlI	CTYRAG	1 cut(s)	1208
SmoI	CTYRAG	1 cut(s)	1208
SphI	GCATGC	1 cut(s)	461
Sse9I	AATT	6 cut(s)	269, 301, 865, 880, 962, 1528
SseBI	AGGCCT	1 cut(s)	1268
SsiI	CCGC	5 cut(s)	66, 87, 292, 754, 1480
SspI	AATATT	1 cut(s)	632
SspMI	CTAG	2 cut(s)	279, 405
SstI	GAGCTC	1 cut(s)	1543
StuI	AGGCCT	1 cut(s)	1268
StyD4I	CCNGG	5 cut(s)	532, 694, 1160, 1268, 1658
StyI	CCWWGG	2 cut(s)	925, 1573
TaaI	ACNGT	9 cut(s)	62, 253, 782, 800, 820, 1040, 1201, 1359, 1594
TaiI	ACGT	1 cut(s)	1566
TaqI	TCGA	5 cut(s)	21, 30, 132, 1513, 1537
TasI	AATT	6 cut(s)	269, 301, 865, 880, 962, 1528
TatI	WGTACW	4 cut(s)	319, 782, 1297, 1368
TauI	GCSGC	3 cut(s)	69, 89, 756
TfiI	GAWTC	4 cut(s)	5, 149, 851, 1568
Tru1I	TTAA	2 cut(s)	522, 597
Tru9I	TTAA	2 cut(s)	522, 597
TscAI	CASTG	7 cut(s)	67, 258, 823, 1235, 1510, 1706, 1750
TseFI	GTSAC	3 cut(s)	160, 1242, 1507
TseI	GCWGC	8 cut(s)	80, 83, 275, 461, 464, 1186, 1584, 1649
Tsp45I	GTSAC	3 cut(s)	160, 1242, 1507
TspRI	CASTG	7 cut(s)	67, 258, 823, 1235, 1510, 1706, 1750
VneI	GTGCAC	1 cut(s)	1202
VpaK11BI	GGWCC	2 cut(s)	1453, 1704
VspI	ATTAAT	2 cut(s)	522, 597
XagI	CCTNNNNNAGG	2 cut(s)	693, 1585
XapI	RAATTY	3 cut(s)	269, 962, 1528
XceI	RCATGY	4 cut(s)	28, 461, 1035, 1349
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1108
XmiI	GTMKAC	1 cut(s)	283
XmnI	GAANNNNTTC	2 cut(s)	351, 1404
XspI	CTAG	2 cut(s)	279, 405
ZrmI	AGTACT	2 cut(s)	1299, 1370
Zsp2I	ATGCAT	3 cut(s)	459, 939, 1033

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.