Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009005

Physical Location & Seq

Genomic Coordinates Forward (+)

32889435 .. 32897128

Gene Structure 7694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1262400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGGATTCGGACGAAGAGTTCGACATGCTCGACGCCAACGACGACGACGACGACGATTTCTACAGTGGCGGCGATGATGATGCAGCTGGCGCTATGGACAGCGACGACGCTGGAGTTGCTGATTACGAGTTCATCGACAACGACTCCGACGATTCCAATGATGTCGCCTCGCATCGATACCAGCAAAACTACACCGTTTTGACAGAGGCTGACATTCGTCAGCATCAAGAGGAGGATATTGTGAGGATATCTACAGTGCTTTCTATTCCAAAAGTTGCAGCTAGCATACTGCTCCGCTACCATAATTGGAGTGTCAGTAAAGTACACGATGAGTGGTTTGCAGATGAAGGAAAGGTTCGCAGGGCTGTTGGCTTGCCCGAGAAACCAGTTGTTGAATACCCTTATGCAAGTGAACTTAATTGTGGGATTTGTTTTGAGACCTATCCTCGTGATAAGATGCATGCTGCAGCTTGTGGTCATCCTTTTTGTTCCTCATGCTGGGCAGGCTATATTAGCACGGCCATTAATGATGGCCCTGGATGTTTGATGTTGCGCTGTCCGGATCCATCTTGTAGTGCTGTTGTTTTCCAAGATATGATAAATGATTTGGCTTCCTATGAAGAGAAGGAGAAATATTCGCGCTACTTTCTTAGATCTTATGTTGAAGACAATAGGAAGACCAAATGGTGTCCTGCTCCAGGCTGTGATTATGCAATAAATTTTATTGTTGGTGGTGGAAGCTATGATGTTACCTGCCACTGTTCATATAGCTTTTGCTGGAATTGTACTGAGGAAGCTCACCGTCCCGTTGATTGCGGCACTGTGTCAAAATGGATAATGAAGAATAGTGCAGAATCTGAAAATATGAATTGGATATTGGCTAATTCCAAGCCCTGCCCAAAGTGCAAACGGCCGATTGAGAAAAACCAAGGCTGTATGCACATCACTTGCACACCACCTTGTAAATTTGAGTTTTGCTGGCTTTGCCTCGGTGCGTGGTCGGACCATGGTGAAAGGACAGGTGGCTTTTATGCATGTAACCGTTATGAGACAGCAAAGCAGGAGGGAGTGTATGATGAGGCAGAGAAACGAAGAGAGATGGCCAAGAACTCTCTGGAGAGGTACACTCATTATTATGAAAGATGGGCAACCAATCAATCGTCCCGTCAAAAAGCACTTGCAGACTTGCAGCAGATGCAAACTGTGCACCTTGAGAAGCTGAGCGATATCCAGTGCCAACCTGAGTCACAGCTGAAGTTCATTACAGAGGCCTGGCTACAGATAGTTGAATGTAGGCGAGTACTAAAATGGACGTATGCTTATGGATATTATTTACCGGAGCATGAACATGCCAAGAGACAGTTCTTTGAGTACTTGCAAGGTGAAGCTGAGTCTGGGTTGGAACGGCTTCATCAATGTGCAGAAAAGGAGCTACAAGTCTACCTTAATGATGAGGACCCATCAAAAGATTTCAATGAGTTCCGCACAAAACTTGCTGGATTGACCAGCGTGACTCGAAACTACTTTGAAAATTTAGTTCGAGCTCTGGAGAATGGTCTATCAGACGTGGATTCCCATGGTGCCTGCAGCAGGACAGTAAGCTCAAAGTGTTTGGGAGGTTCAAGTAAGGGAAGAGGTGGGAGGGTCAAGGCTACAACATCCAGGTCTAGTAGTTCGAGTAGAAACATTGACGATTTAGGCCACTGGTCCTGTGAACATTGCACCTTCGCAAATGTCAGGTCGGCCAGTGTGTGCCAGATGTGTCAACAGCGCCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

IBR zf-C3HC4

Protein Analysis

593

Amino Acids

67.41

Weight (kDa)

5.11

Isoelectric Point (pI)

55.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_ARI1	PF21235	74 - 113	1.1e-08	E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR	PF01485	210 - 272	2.2e-16	IBR domain, a half RING-finger domain
IBR	PF01485	289 - 336	5.4e-07	IBR domain, a half RING-finger domain
IBR_1	PF22191	294 - 334	2.7e-14	IBR domain
IBR_2	PF22605	295 - 334	4.4e-12	IBR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65430
fragaria_vesca	FvH4_6g00130 FvH4_6g00130
malus_domestica	MD04G1248300.v1.1 MD12G1262400.v1.1
prunus_persica	Prupe.6G364600_v2.0.a1
pyrus_communis	pycom04g21900 pycom12g24160
rosa_chinensis	RchiOBHm_Chr2g0127711 RchiOBHm_Chr3g0447471 RchiOBHm_Chr6g0267381
rosa_laevigata	RLG00000025995
rosa_multiflora	Rmu_sc0006572.1_g000005
rosa_roxburghii	Rroxscaffold_5G00344110 Rroxscaffold_6G00425690
rosa_rugosa	Rorug02G0602100
rosa_samantha	Rh2BG278300 Rh3AG002300 Rh3BG001800 Rh3CG002100 Rh3DG002200
rosa_wichuraiana	Rw3G000120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	761
AccB1I	GGYRCC	1 cut(s)	1580
AccI	GTMKAC	1 cut(s)	1440
AccII	CGCG	1 cut(s)	640
AccIII	TCCGGA	1 cut(s)	559
AciI	CCGC	4 cut(s)	69, 295, 816, 1483
AclWI	GGATC	2 cut(s)	557, 570
AcoI	YGGCCR	4 cut(s)	519, 911, 1101, 1743
AcsI	RAATTY	3 cut(s)	718, 965, 1531
AcuI	CTGAAG	1 cut(s)	1274
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	5 cut(s)	324, 787, 1124, 1302, 1373
AfiI	CCNNNNNNNGG	3 cut(s)	498, 698, 1590
AgsI	TTSAA	6 cut(s)	395, 665, 1289, 1474, 1529, 1623
AjiI	CACGTC	1 cut(s)	1567
AjnI	CCWGG	4 cut(s)	535, 697, 1271, 1661
AjuI	GAANNNNNNNTTGG	4 cut(s)	860, 892, 1346, 1378
AloI	GAACNNNNNNTCC	1 cut(s)	34
Alw21I	GWGCWC	2 cut(s)	1209, 1546
Alw26I	GTCTC	3 cut(s)	431, 1043, 1351
Alw44I	GTGCAC	1 cut(s)	1205
AlwI	GGATC	2 cut(s)	557, 570
AlwNI	CAGNNNCTG	2 cut(s)	209, 857
Ama87I	CYCGRG	1 cut(s)	377
Aor13HI	TCCGGA	1 cut(s)	559
AoxI	GGCC	7 cut(s)	519, 532, 911, 1101, 1269, 1699, 1743
ApaLI	GTGCAC	1 cut(s)	1205
ApeKI	GCWGC	6 cut(s)	83, 278, 464, 467, 1189, 1587
ApoI	RAATTY	3 cut(s)	718, 965, 1531
ArsI	GACNNNNNNTTYG	3 cut(s)	34, 1724, 1756
AseI	ATTAAT	1 cut(s)	525
Asp700I	GAANNNNTTC	1 cut(s)	1407
AspLEI	GCGC	4 cut(s)	92, 555, 642, 1773
AspS9I	GGNCC	4 cut(s)	533, 1003, 1456, 1707
AsuHPI	GGTGA	3 cut(s)	791, 1022, 1394
AsuNHI	GCTAGC	1 cut(s)	281
AvaI	CYCGRG	1 cut(s)	377
AvaII	GGWCC	3 cut(s)	1003, 1456, 1707
BaeGI	GKGCMC	1 cut(s)	1209
BalI	TGGCCA	1 cut(s)	1103
BamHI	GGATCC	1 cut(s)	562
BanI	GGYRCC	1 cut(s)	1580
BanII	GRGCYC	1 cut(s)	1546
BauI	CACGAG	1 cut(s)	447
BbsI	GAAGAC	2 cut(s)	672, 683
Bbv12I	GWGCWC	2 cut(s)	1209, 1546
BbvI	GCAGC	6 cut(s)	95, 290, 451, 479, 1201, 1599
BccI	CCATC	5 cut(s)	524, 574, 1093, 1137, 1468
BceAI	ACGGC	3 cut(s)	534, 926, 1421
BcgI	CGANNNNNNTGC	2 cut(s)	62, 96
BciT130I	CCWGG	4 cut(s)	537, 699, 1273, 1663
BcoDI	GTCTC	3 cut(s)	431, 1043, 1351
BfaI	CTAG	2 cut(s)	282, 1668
BfmI	CTRYAG	5 cut(s)	61, 252, 465, 1277, 1585
BfoI	RGCGCY	2 cut(s)	93, 1774
BfuAI	ACCTGC	1 cut(s)	761
BglII	AGATCT	1 cut(s)	653
BisI	GCNGC	8 cut(s)	70, 84, 279, 465, 468, 817, 1190, 1588
BlpI	GCTNAGC	1 cut(s)	1220
BlsI	GCNGC	8 cut(s)	71, 85, 280, 466, 469, 818, 1191, 1589
BmcAI	AGTACT	2 cut(s)	1302, 1373
Bme1390I	CCNGG	4 cut(s)	537, 699, 1273, 1663
Bme18I	GGWCC	3 cut(s)	1003, 1456, 1707
BmeT110I	CYCGRG	1 cut(s)	377
BmgBI	CACGTC	1 cut(s)	1567
BmgT120I	GGNCC	4 cut(s)	533, 1003, 1456, 1707
BmiI	GGNNCC	3 cut(s)	564, 1458, 1582
BmrFI	CCNGG	4 cut(s)	537, 699, 1273, 1663
BmsI	GCATC	5 cut(s)	70, 181, 232, 447, 1185
BmtI	GCTAGC	1 cut(s)	285
BoxI	GACNNNNGTC	1 cut(s)	216
BpiI	GAAGAC	2 cut(s)	672, 683
BplI	GAGNNNNNCTC	2 cut(s)	1111, 1143
BpmI	CTGGAG	4 cut(s)	132, 681, 1136, 1568
Bpu1102I	GCTNAGC	1 cut(s)	1220
BpuEI	CTTGAG	1 cut(s)	1232
Bsa29I	ATCGAT	1 cut(s)	175
BsaHI	GRCGYC	1 cut(s)	33
BsaI	GGTCTC	1 cut(s)	431
BsaJI	CCNNGG	5 cut(s)	535, 928, 988, 1006, 1576
BsaWI	WCCGGW	2 cut(s)	559, 1336
BsaXI	ACNNNNNCTCC	6 cut(s)	128, 158, 301, 331, 1055, 1085
Bsc4I	CCNNNNNNNGG	3 cut(s)	498, 698, 1590
Bse118I	RCCGGY	1 cut(s)	1773
Bse1I	ACTGG	4 cut(s)	386, 1231, 1709, 1746
Bse3DI	GCAATG	1 cut(s)	1717
BseAI	TCCGGA	1 cut(s)	559
BseBI	CCWGG	4 cut(s)	537, 699, 1273, 1663
BseCI	ATCGAT	1 cut(s)	175
BseDI	CCNNGG	5 cut(s)	535, 928, 988, 1006, 1576
BseGI	GGATG	3 cut(s)	478, 545, 1658
BseLI	CCNNNNNNNGG	3 cut(s)	498, 698, 1590
BseMI	GCAATG	1 cut(s)	1717
BseMII	CTCAG	4 cut(s)	780, 1211, 1233, 1380
BseNI	ACTGG	4 cut(s)	386, 1231, 1709, 1746
BseRI	GAGGAG	1 cut(s)	245
BseSI	GKGCMC	1 cut(s)	1209
BseX3I	CGGCCG	1 cut(s)	911
BseXI	GCAGC	6 cut(s)	95, 290, 451, 479, 1201, 1599
BseYI	CCCAGC	1 cut(s)	498
BsgI	GTGCAG	2 cut(s)	870, 1440
Bsh1236I	CGCG	1 cut(s)	640
Bsh1285I	CGRYCG	1 cut(s)	914
BshFI	GGCC	7 cut(s)	521, 534, 913, 1103, 1271, 1701, 1745
BshNI	GGYRCC	1 cut(s)	1580
BshVI	ATCGAT	1 cut(s)	175
BsiEI	CGRYCG	1 cut(s)	914
BsiHKAI	GWGCWC	2 cut(s)	1209, 1546
BsiHKCI	CYCGRG	1 cut(s)	377
BsiSI	CCGG	3 cut(s)	560, 1337, 1774
BslFI	GGGAC	2 cut(s)	789, 1147
BslI	CCNNNNNNNGG	3 cut(s)	498, 698, 1590
BsmAI	GTCTC	3 cut(s)	431, 1043, 1351
BsmFI	GGGAC	2 cut(s)	789, 1147
BsnI	GGCC	7 cut(s)	521, 534, 913, 1103, 1271, 1701, 1745
Bso31I	GGTCTC	1 cut(s)	431
BsoBI	CYCGRG	1 cut(s)	377
Bsp1286I	GDGCHC	2 cut(s)	1209, 1546
Bsp13I	TCCGGA	1 cut(s)	559
Bsp143I	GATC	2 cut(s)	562, 653
Bsp1720I	GCTNAGC	1 cut(s)	1220
Bsp19I	CCATGG	2 cut(s)	1006, 1576
BspACI	CCGC	4 cut(s)	69, 295, 816, 1483
BspANI	GGCC	7 cut(s)	521, 534, 913, 1103, 1271, 1701, 1745
BspCNI	CTCAG	4 cut(s)	781, 1212, 1234, 1381
BspDI	ATCGAT	1 cut(s)	175
BspEI	TCCGGA	1 cut(s)	559
BspFNI	CGCG	1 cut(s)	640
BspLI	GGNNCC	3 cut(s)	564, 1458, 1582
BspMAI	CTGCAG	2 cut(s)	469, 1589
BspMI	ACCTGC	1 cut(s)	761
BspOI	GCTAGC	1 cut(s)	285
BspPI	GGATC	2 cut(s)	557, 570
BspT107I	GGYRCC	1 cut(s)	1580
BspTNI	GGTCTC	1 cut(s)	431
BsrDI	GCAATG	1 cut(s)	1717
BsrFI	RCCGGY	1 cut(s)	1773
BsrI	ACTGG	4 cut(s)	386, 1231, 1709, 1746
BssAI	RCCGGY	1 cut(s)	1773
BssECI	CCNNGG	5 cut(s)	535, 928, 988, 1006, 1576
BssMI	GATC	2 cut(s)	562, 653
BssNI	GRCGYC	1 cut(s)	33
BssSI	CACGAG	1 cut(s)	447
BssT1I	CCWWGG	3 cut(s)	928, 1006, 1576
Bst2BI	CACGAG	1 cut(s)	447
Bst2UI	CCWGG	4 cut(s)	537, 699, 1273, 1663
Bst6I	CTCTTC	4 cut(s)	9, 615, 1087, 1627
BstACI	GRCGYC	1 cut(s)	33
BstAPI	GCANNNNNTGC	2 cut(s)	1195, 1204
BstC8I	GCNNGC	7 cut(s)	88, 283, 374, 462, 505, 980, 1585
BstDEI	CTNAG	5 cut(s)	650, 789, 1220, 1242, 1389
BstDSI	CCRYGG	2 cut(s)	1006, 1576
BstENI	CCTNNNNNAGG	2 cut(s)	696, 1588
BstF5I	GGATG	3 cut(s)	478, 545, 1658
BstFNI	CGCG	1 cut(s)	640
BstH2I	RGCGCY	2 cut(s)	93, 1774
BstHHI	GCGC	4 cut(s)	92, 555, 642, 1773
BstKTI	GATC	2 cut(s)	565, 656
BstMAI	GTCTC	3 cut(s)	431, 1043, 1351
BstMBI	GATC	2 cut(s)	562, 653
BstMCI	CGRYCG	1 cut(s)	914
BstMWI	GCNNNNNNNGC	8 cut(s)	89, 116, 504, 513, 903, 984, 1195, 1204
BstNI	CCWGG	4 cut(s)	537, 699, 1273, 1663
BstNSI	RCATGY	4 cut(s)	28, 464, 1038, 1352
BstPAI	GACNNNNGTC	1 cut(s)	216
BstSCI	CCNGG	4 cut(s)	535, 697, 1271, 1661
BstSFI	CTRYAG	5 cut(s)	61, 252, 465, 1277, 1585
BstSLI	GKGCMC	1 cut(s)	1209
BstUI	CGCG	1 cut(s)	640
BstV1I	GCAGC	6 cut(s)	95, 290, 451, 479, 1201, 1599
BstV2I	GAAGAC	2 cut(s)	672, 683
BstX2I	RGATCY	2 cut(s)	562, 653
BstYI	RGATCY	2 cut(s)	562, 653
BstZI	CGGCCG	1 cut(s)	911
Bsu15I	ATCGAT	1 cut(s)	175
BsuRI	GGCC	7 cut(s)	521, 534, 913, 1103, 1271, 1701, 1745
BsuTUI	ATCGAT	1 cut(s)	175
BtgI	CCRYGG	2 cut(s)	1006, 1576
BtgZI	GCGATG	1 cut(s)	87
BtrI	CACGTC	1 cut(s)	1567
BtsCI	GGATG	3 cut(s)	478, 545, 1658
BtsIMutI	CAGTG	7 cut(s)	70, 261, 757, 819, 1238, 1702, 1753
BveI	ACCTGC	1 cut(s)	761
Cac8I	GCNNGC	7 cut(s)	88, 283, 374, 462, 505, 980, 1585
CaiI	CAGNNNCTG	2 cut(s)	209, 857
CfoI	GCGC	4 cut(s)	92, 555, 642, 1773
Cfr10I	RCCGGY	1 cut(s)	1773
Cfr13I	GGNCC	4 cut(s)	533, 1003, 1456, 1707
ClaI	ATCGAT	1 cut(s)	175
CseI	GACGC	2 cut(s)	41, 116
Csp6I	GTAC	5 cut(s)	323, 786, 1123, 1301, 1372
CviAII	CATG	8 cut(s)	25, 461, 495, 1007, 1035, 1343, 1349, 1577
CviQI	GTAC	5 cut(s)	323, 786, 1123, 1301, 1372
DdeI	CTNAG	5 cut(s)	650, 789, 1220, 1242, 1389
DpnI	GATC	2 cut(s)	564, 655
DpnII	GATC	2 cut(s)	562, 653
EaeI	YGGCCR	4 cut(s)	519, 911, 1101, 1743
EagI	CGGCCG	1 cut(s)	911
Eam1104I	CTCTTC	4 cut(s)	9, 615, 1087, 1627
EarI	CTCTTC	4 cut(s)	9, 615, 1087, 1627
Ecl136II	GAGCTC	1 cut(s)	1544
EclXI	CGGCCG	1 cut(s)	911
Eco130I	CCWWGG	3 cut(s)	928, 1006, 1576
Eco147I	AGGCCT	1 cut(s)	1271
Eco24I	GRGCYC	1 cut(s)	1546
Eco31I	GGTCTC	1 cut(s)	431
Eco32I	GATATC	2 cut(s)	249, 1228
Eco47I	GGWCC	3 cut(s)	1003, 1456, 1707
Eco52I	CGGCCG	1 cut(s)	911
Eco53kI	GAGCTC	1 cut(s)	1544
Eco57I	CTGAAG	1 cut(s)	1274
Eco88I	CYCGRG	1 cut(s)	377
EcoICRI	GAGCTC	1 cut(s)	1544
EcoNI	CCTNNNNNAGG	2 cut(s)	696, 1588
EcoO109I	RGGNCCY	1 cut(s)	1456
EcoRII	CCWGG	4 cut(s)	535, 697, 1271, 1661
EcoRV	GATATC	2 cut(s)	249, 1228
EcoT14I	CCWWGG	3 cut(s)	928, 1006, 1576
EcoT22I	ATGCAT	2 cut(s)	462, 1036
EcoT38I	GRGCYC	1 cut(s)	1546
ErhI	CCWWGG	3 cut(s)	928, 1006, 1576
FaeI	CATG	8 cut(s)	28, 464, 498, 1010, 1038, 1346, 1352, 1580
FalI	AAGNNNNNCTT	2 cut(s)	1428, 1460
FaqI	GGGAC	2 cut(s)	789, 1147
FatI	CATG	8 cut(s)	24, 460, 494, 1006, 1034, 1342, 1348, 1576
FblI	GTMKAC	1 cut(s)	1440
Fnu4HI	GCNGC	8 cut(s)	70, 84, 279, 465, 468, 817, 1190, 1588
FokI	GGATG	3 cut(s)	465, 552, 1645
FriOI	GRGCYC	1 cut(s)	1546
Fsp4HI	GCNGC	8 cut(s)	70, 84, 279, 465, 468, 817, 1190, 1588
FspBI	CTAG	2 cut(s)	282, 1668
GlaI	GCGC	4 cut(s)	91, 554, 641, 1772
GluI	GCNGC	8 cut(s)	70, 84, 279, 465, 468, 817, 1190, 1588
GsaI	CCCAGC	1 cut(s)	502
GsuI	CTGGAG	4 cut(s)	132, 681, 1136, 1568
HaeII	RGCGCY	2 cut(s)	93, 1774
HaeIII	GGCC	7 cut(s)	521, 534, 913, 1103, 1271, 1701, 1745
HapII	CCGG	3 cut(s)	560, 1337, 1774
HgaI	GACGC	2 cut(s)	41, 116
HhaI	GCGC	4 cut(s)	92, 555, 642, 1773
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	8 cut(s)	28, 464, 498, 1010, 1038, 1346, 1352, 1580
Hin6I	GCGC	4 cut(s)	90, 553, 640, 1771
HinP1I	GCGC	4 cut(s)	90, 553, 640, 1771
HincII	GTYRAC	1 cut(s)	1766
HindII	GTYRAC	1 cut(s)	1766
HinfI	GANTC	8 cut(s)	5, 143, 152, 854, 1244, 1391, 1513, 1571
HpaII	CCGG	3 cut(s)	560, 1337, 1774
HphI	GGTGA	3 cut(s)	791, 1022, 1394
Hpy166II	GTNNAC	7 cut(s)	325, 413, 1125, 1207, 1441, 1715, 1766
Hpy188I	TCNGA	5 cut(s)	10, 148, 859, 1003, 1564
Hpy188III	TCNNGA	5 cut(s)	227, 449, 560, 1115, 1547
Hpy8I	GTNNAC	7 cut(s)	325, 413, 1125, 1207, 1441, 1715, 1766
Hpy99I	CGWCG	9 cut(s)	35, 44, 47, 50, 53, 56, 107, 110, 152
HpyAV	CCTTC	3 cut(s)	341, 619, 1735
HpyCH4IV	ACGT	2 cut(s)	1313, 1566
HpyF10VI	GCNNNNNNNGC	8 cut(s)	89, 116, 504, 513, 903, 984, 1195, 1204
HpyF3I	CTNAG	5 cut(s)	650, 789, 1220, 1242, 1389
HpySE526I	ACGT	2 cut(s)	1313, 1566
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	8 cut(s)	28, 464, 498, 1010, 1038, 1346, 1352, 1580
HspAI	GCGC	4 cut(s)	90, 553, 640, 1771
Kpn2I	TCCGGA	1 cut(s)	559
Kzo9I	GATC	2 cut(s)	562, 653
LmnI	GCTCC	4 cut(s)	297, 700, 1339, 1429
Lsp1109I	GCAGC	6 cut(s)	95, 290, 451, 479, 1201, 1599
LweI	GCATC	5 cut(s)	70, 181, 232, 447, 1185
MaeI	CTAG	2 cut(s)	282, 1668
MaeII	ACGT	2 cut(s)	1313, 1566
MaeIII	GTNAC	4 cut(s)	748, 1037, 1245, 1510
MalI	GATC	2 cut(s)	564, 655
MboI	GATC	2 cut(s)	562, 653
MboII	GAAGA	7 cut(s)	26, 632, 677, 688, 853, 1104, 1644
MflI	RGATCY	2 cut(s)	562, 653
MhlI	GDGCHC	2 cut(s)	1209, 1546
MlsI	TGGCCA	1 cut(s)	1103
MluCI	AATT	8 cut(s)	304, 418, 718, 781, 868, 883, 965, 1531
MluNI	TGGCCA	1 cut(s)	1103
MlyI	GAGTC	4 cut(s)	137, 1253, 1400, 1507
MmeI	TCCRAC	3 cut(s)	171, 981, 1380
Mox20I	TGGCCA	1 cut(s)	1103
Mph1103I	ATGCAT	2 cut(s)	462, 1036
MroI	TCCGGA	1 cut(s)	559
MroXI	GAANNNNTTC	1 cut(s)	1407
MscI	TGGCCA	1 cut(s)	1103
MseI	TTAA	3 cut(s)	417, 525, 1446
MslI	CAYNNNNRTG	3 cut(s)	1134, 1347, 1416
Msp20I	TGGCCA	1 cut(s)	1103
MspA1I	CMGCKG	2 cut(s)	86, 1252
MspI	CCGG	3 cut(s)	560, 1337, 1774
MspR9I	CCNGG	4 cut(s)	537, 699, 1273, 1663
MvaI	CCWGG	4 cut(s)	537, 699, 1273, 1663
MvnI	CGCG	1 cut(s)	640
MwoI	GCNNNNNNNGC	8 cut(s)	89, 116, 504, 513, 903, 984, 1195, 1204
NcoI	CCATGG	2 cut(s)	1006, 1576
NdeII	GATC	2 cut(s)	562, 653
NheI	GCTAGC	1 cut(s)	281
NlaIII	CATG	8 cut(s)	28, 464, 498, 1010, 1038, 1346, 1352, 1580
NlaIV	GGNNCC	3 cut(s)	564, 1458, 1582
NmuCI	GTSAC	2 cut(s)	1245, 1510
NsiI	ATGCAT	2 cut(s)	462, 1036
NspI	RCATGY	4 cut(s)	28, 464, 1038, 1352
PaeI	GCATGC	1 cut(s)	464
PceI	AGGCCT	1 cut(s)	1271
PcsI	WCGNNNNNNNCGW	9 cut(s)	18, 27, 36, 39, 45, 48, 51, 132, 147
PdmI	GAANNNNTTC	1 cut(s)	1407
PfeI	GAWTC	4 cut(s)	5, 152, 854, 1571
PkrI	GCNGC	8 cut(s)	71, 85, 280, 466, 469, 818, 1191, 1589
PleI	GAGTC	4 cut(s)	137, 1252, 1399, 1507
PpsI	GAGTC	4 cut(s)	137, 1252, 1399, 1507
PpuMI	RGGWCCY	1 cut(s)	1456
PshAI	GACNNNNGTC	1 cut(s)	216
PshBI	ATTAAT	1 cut(s)	525
Psp124BI	GAGCTC	1 cut(s)	1546
Psp5II	RGGWCCY	1 cut(s)	1456
Psp6I	CCWGG	4 cut(s)	535, 697, 1271, 1661
PspFI	CCCAGC	1 cut(s)	498
PspGI	CCWGG	4 cut(s)	535, 697, 1271, 1661
PspN4I	GGNNCC	3 cut(s)	564, 1458, 1582
PspPI	GGNCC	4 cut(s)	533, 1003, 1456, 1707
PspPPI	RGGWCCY	1 cut(s)	1456
PstI	CTGCAG	2 cut(s)	469, 1589
PstNI	CAGNNNCTG	2 cut(s)	209, 857
PsuI	RGATCY	2 cut(s)	562, 653
PvuII	CAGCTG	2 cut(s)	86, 1252
RsaI	GTAC	5 cut(s)	324, 787, 1124, 1302, 1373
RsaNI	GTAC	5 cut(s)	323, 786, 1123, 1301, 1372
RseI	CAYNNNNRTG	3 cut(s)	1134, 1347, 1416
SacI	GAGCTC	1 cut(s)	1546
SaqAI	TTAA	3 cut(s)	417, 525, 1446
SatI	GCNGC	8 cut(s)	70, 84, 279, 465, 468, 817, 1190, 1588
Sau3AI	GATC	2 cut(s)	562, 653
Sau96I	GGNCC	4 cut(s)	533, 1003, 1456, 1707
ScaI	AGTACT	2 cut(s)	1302, 1373
SchI	GAGTC	4 cut(s)	137, 1253, 1400, 1507
ScrFI	CCNGG	4 cut(s)	537, 699, 1273, 1663
SduI	GDGCHC	2 cut(s)	1209, 1546
SfaNI	GCATC	5 cut(s)	70, 181, 232, 447, 1185
SfcI	CTRYAG	5 cut(s)	61, 252, 465, 1277, 1585
SinI	GGWCC	3 cut(s)	1003, 1456, 1707
SmiMI	CAYNNNNRTG	3 cut(s)	1134, 1347, 1416
SmlI	CTYRAG	1 cut(s)	1211
SmoI	CTYRAG	1 cut(s)	1211
SphI	GCATGC	1 cut(s)	464
Sse9I	AATT	8 cut(s)	304, 418, 718, 781, 868, 883, 965, 1531
SseBI	AGGCCT	1 cut(s)	1271
SsiI	CCGC	4 cut(s)	69, 295, 816, 1483
SspI	AATATT	1 cut(s)	635
SspMI	CTAG	2 cut(s)	282, 1668
SstI	GAGCTC	1 cut(s)	1546
StuI	AGGCCT	1 cut(s)	1271
StyD4I	CCNGG	4 cut(s)	535, 697, 1271, 1661
StyI	CCWWGG	3 cut(s)	928, 1006, 1576
TaiI	ACGT	2 cut(s)	1316, 1569
TaqI	TCGA	7 cut(s)	21, 30, 135, 175, 1516, 1540, 1676
TasI	AATT	8 cut(s)	304, 418, 718, 781, 868, 883, 965, 1531
TatI	WGTACW	4 cut(s)	322, 785, 1300, 1371
TauI	GCSGC	2 cut(s)	72, 819
TfiI	GAWTC	4 cut(s)	5, 152, 854, 1571
Tru1I	TTAA	3 cut(s)	417, 525, 1446
Tru9I	TTAA	3 cut(s)	417, 525, 1446
TscAI	CASTG	7 cut(s)	70, 261, 764, 826, 1238, 1709, 1753
TseFI	GTSAC	2 cut(s)	1245, 1510
TseI	GCWGC	6 cut(s)	83, 278, 464, 467, 1189, 1587
Tsp45I	GTSAC	2 cut(s)	1245, 1510
TspRI	CASTG	7 cut(s)	70, 261, 764, 826, 1238, 1709, 1753
VneI	GTGCAC	1 cut(s)	1205
VpaK11BI	GGWCC	3 cut(s)	1003, 1456, 1707
VspI	ATTAAT	1 cut(s)	525
XagI	CCTNNNNNAGG	2 cut(s)	696, 1588
XapI	RAATTY	3 cut(s)	718, 965, 1531
XceI	RCATGY	4 cut(s)	28, 464, 1038, 1352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1111
XmiI	GTMKAC	1 cut(s)	1440
XmnI	GAANNNNTTC	1 cut(s)	1407
XspI	CTAG	2 cut(s)	282, 1668
ZrmI	AGTACT	2 cut(s)	1302, 1373
Zsp2I	ATGCAT	2 cut(s)	462, 1036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.