MD05G1012500.v1.1

psbP domain-containing protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014267
Physical Location & Seq
Reverse (-)
2489133 .. 2491368
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1012500.v1.1.491

Sequence Viewer

Length: 834 bp
ATGAGAACAGCCTCACTTACCGGTTGGAGCTTCTCCTGGACTCATCAGCGGCAAGCAATTCCTTCCCACAATTTGGTTGTTCAGTCTTCATTGGAGCATGGAAATTGCAGGACAAAAGTTGGCTCAAGTTCGACAGAGAGTGGCTTGGTTGATGGAGAAGCTGAGAATTTCTCCGGGATTTTGAAGAGACGATCAGTGTTGCTTTCTGCAGGAGCTTCTTTGTTTTCTTCTCAAGTGTTGGGTTTTCCGAGAGAGGTATTGGCAGTGGTGAAACAAGGTCTTCTAGCCGGCCGGATTCCCGGTCTGTCCGAACCGGATGAACAAGGTTGGAGGACATACCAAAGACCAGATGAGAAGTCCGGAGGGCATGGCGTTGGTTGGAGCCCTATCATCCCTTATCGCTTTTCAGTTCCTCAAGATTGGGAAGAGGTTCCAGTATCAATAGCGGATCTAGGCGGTACAGAGATCGATTTGAGATTTGGTAGCCCAAAGGAGGGACGCATTTCTGTCATTGTCGCTCCTGTTCTGAGATTTGCAGATAATTTAGATGGAGATGCCACAATTGAGAAAATTGGACGTCCAGAGACGGTAATAAATGCATTTGGACCCGAAGTTATTGGCGAAAATGTTGAAGGAAAGGTTATGAGCATAACTGTTGTACAAGACTCTGGAAGAACATATTACCAGTATGAGTTGGAGCCACCTCATGCTCTGATAACAGCAACTGCAGCTGGGAACCGCCTGTACTTGTTTAGCGTAACTGGAAGTGGTCTTCAATGGAAGAGGCATTACAAGGACTTGAAGAAAATAGCCGACTCCTTCCGTGTCGTCTAA

Protein Analysis

278

Amino Acids

30.27

Weight (kDa)

6.33

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 133 - 276 2.8e-12 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77090
fragaria_vesca FvH4_2g14791
malus_domestica MD05G1012500.v1.1 MD10G1012400.v1.1
prunus_persica Prupe.8G017800_v2.0.a1
pyrus_communis pycom10g00810
rosa_chinensis RchiOBHm_Chr6g0277361
rosa_laevigata RLG00000013307 RLG00000029732
rosa_multiflora Rmu_sc0000030.1_g000012
rosa_roxburghii Rroxscaffold_7G00191400
rosa_rugosa Rorug01G0093300
rosa_samantha Rh6AG218500 Rh6BG222200 Rh6CG225000 Rh6DG215200
rosa_wichuraiana Rw6G019140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 73
AccIII TCCGGA 1 cut(s) 359
AciI CCGC 4 cut(s) 49, 446, 456, 739
AclWI GGATC 1 cut(s) 456
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 1 cut(s) 166
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 3 cut(s) 460, 660, 746
AfiI CCNNNNNNNGG 3 cut(s) 73, 493, 494
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 4 cut(s) 184, 632, 776, 802
AjnI CCWGG 1 cut(s) 35
AluBI AGCT 4 cut(s) 30, 161, 215, 731
AluI AGCT 4 cut(s) 30, 161, 215, 731
Alw26I GTCTC 2 cut(s) 181, 578
AlwI GGATC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 725
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 1 cut(s) 166
AsiGI ACCGGT 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 2 cut(s) 175, 300
AsuHPI GGTGA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 386
BarI GAAGNNNNNNTAC 2 cut(s) 773, 805
BbsI GAAGAC 3 cut(s) 78, 272, 764
BbvI GCAGC 1 cut(s) 740
BccI CCATC 2 cut(s) 146, 542
BciT130I CCWGG 1 cut(s) 37
BcnI CCSGG 2 cut(s) 175, 300
BcoDI GTCTC 2 cut(s) 181, 578
BfaI CTAG 2 cut(s) 284, 452
BfmI CTRYAG 2 cut(s) 207, 726
BisI GCNGC 2 cut(s) 50, 729
BlsI GCNGC 2 cut(s) 51, 730
Bme1390I CCNGG 3 cut(s) 37, 175, 300
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 5 cut(s) 383, 432, 607, 699, 737
BmrFI CCNGG 3 cut(s) 37, 175, 300
BmsI GCATC 1 cut(s) 544
BpiI GAAGAC 3 cut(s) 78, 272, 764
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpuEI CTTGAG 3 cut(s) 109, 216, 399
BpuMI CCSGG 2 cut(s) 175, 300
Bsa29I ATCGAT 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 577
BsaWI WCCGGW 3 cut(s) 20, 313, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 493, 494
Bse118I RCCGGY 2 cut(s) 20, 287
Bse1I ACTGG 3 cut(s) 434, 685, 766
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 1 cut(s) 37
BseCI ATCGAT 1 cut(s) 468
BseGI GGATG 2 cut(s) 322, 390
BseLI CCNNNNNNNGG 3 cut(s) 73, 493, 494
BseMII CTCAG 2 cut(s) 153, 518
BseNI ACTGG 3 cut(s) 434, 685, 766
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 740
BseYI CCCAGC 1 cut(s) 731
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 1 cut(s) 291
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 292
BsiSI CCGG 7 cut(s) 21, 174, 288, 292, 300, 314, 360
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 3 cut(s) 73, 493, 494
BsmAI GTCTC 2 cut(s) 181, 578
BsmBI CGTCTC 2 cut(s) 181, 578
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 386
Bsp13I TCCGGA 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 3 cut(s) 191, 448, 465
BspACI CCGC 4 cut(s) 49, 446, 456, 739
BspANI GGCC 1 cut(s) 291
BspCNI CTCAG 2 cut(s) 154, 519
BspDI ATCGAT 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 359
BspLI GGNNCC 5 cut(s) 383, 432, 607, 699, 737
BspMAI CTGCAG 2 cut(s) 211, 730
BspPI GGATC 1 cut(s) 456
BsrFI RCCGGY 2 cut(s) 20, 287
BsrGI TGTACA 1 cut(s) 658
BsrI ACTGG 3 cut(s) 434, 685, 766
BssAI RCCGGY 2 cut(s) 20, 287
BssMI GATC 3 cut(s) 191, 448, 465
BssNI GRCGYC 1 cut(s) 577
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 589, 655
Bst6I CTCTTC 3 cut(s) 179, 420, 776
BstACI GRCGYC 1 cut(s) 577
BstAUI TGTACA 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 54, 289
BstDEI CTNAG 2 cut(s) 162, 527
BstF5I GGATG 2 cut(s) 322, 390
BstKTI GATC 3 cut(s) 194, 451, 468
BstMAI GTCTC 2 cut(s) 181, 578
BstMBI GATC 3 cut(s) 191, 448, 465
BstMCI CGRYCG 1 cut(s) 292
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNI CCWGG 1 cut(s) 37
BstSCI CCNGG 3 cut(s) 35, 173, 298
BstSFI CTRYAG 2 cut(s) 207, 726
BstV1I GCAGC 1 cut(s) 740
BstV2I GAAGAC 3 cut(s) 78, 272, 764
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZI CGGCCG 1 cut(s) 289
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 1 cut(s) 291
BsuTUI ATCGAT 1 cut(s) 468
BtsCI GGATG 2 cut(s) 322, 390
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 201, 270
Cac8I GCNNGC 2 cut(s) 54, 289
CaiI CAGNNNCTG 1 cut(s) 725
Cfr10I RCCGGY 2 cut(s) 20, 287
Cfr13I GGNCC 1 cut(s) 605
ClaI ATCGAT 1 cut(s) 468
CseI GACGC 1 cut(s) 507
Csp6I GTAC 3 cut(s) 459, 659, 745
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 3 cut(s) 98, 368, 707
CviQI GTAC 3 cut(s) 459, 659, 745
DdeI CTNAG 2 cut(s) 162, 527
DpnI GATC 3 cut(s) 193, 450, 467
DpnII GATC 3 cut(s) 191, 448, 465
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
Eam1104I CTCTTC 3 cut(s) 179, 420, 776
EarI CTCTTC 3 cut(s) 179, 420, 776
EclXI CGGCCG 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 605
Eco52I CGGCCG 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 386
Esp3I CGTCTC 2 cut(s) 181, 578
FaeI CATG 3 cut(s) 101, 371, 710
FaiI YATR 8 cut(s) 99, 337, 369, 644, 650, 679, 690, 708
FaqI GGGAC 1 cut(s) 510
FatI CATG 3 cut(s) 97, 367, 706
Fnu4HI GCNGC 2 cut(s) 50, 729
FokI GGATG 2 cut(s) 329, 377
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 50, 729
FspBI CTAG 2 cut(s) 284, 452
GluI GCNGC 2 cut(s) 50, 729
GsaI CCCAGC 1 cut(s) 735
HaeIII GGCC 1 cut(s) 291
HapII CCGG 7 cut(s) 21, 174, 288, 292, 300, 314, 360
HgaI GACGC 1 cut(s) 507
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 3 cut(s) 101, 371, 710
HinfI GANTC 4 cut(s) 40, 295, 665, 815
HpaII CCGG 7 cut(s) 21, 174, 288, 292, 300, 314, 360
HphI GGTGA 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 249, 310, 528, 714
Hpy188III TCNNGA 4 cut(s) 360, 416, 581, 669
HpyAV CCTTC 3 cut(s) 72, 626, 829
HpyCH4III ACNGT 2 cut(s) 589, 655
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 5 cut(s) 108, 209, 536, 599, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 2 cut(s) 162, 527
HpySE526I ACGT 1 cut(s) 577
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 3 cut(s) 101, 371, 710
Kpn2I TCCGGA 1 cut(s) 359
KroI GCCGGC 1 cut(s) 287
KroNI GCCGGC 1 cut(s) 289
Kzo9I GATC 3 cut(s) 191, 448, 465
LmnI GCTCC 6 cut(s) 27, 94, 212, 381, 523, 697
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 1 cut(s) 544
MaeI CTAG 2 cut(s) 284, 452
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 1 cut(s) 757
MalI GATC 3 cut(s) 193, 450, 467
MboI GATC 3 cut(s) 191, 448, 465
MboII GAAGA 9 cut(s) 78, 196, 219, 272, 437, 684, 764, 793, 814
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 7 cut(s) 57, 70, 103, 166, 541, 561, 570
MlyI GAGTC 3 cut(s) 34, 659, 809
MmeI TCCRAC 4 cut(s) 5, 308, 359, 675
MnlI CCTC 9 cut(s) 22, 247, 324, 356, 421, 423, 487, 714, 777
Mph1103I ATGCAT 1 cut(s) 601
MroI TCCGGA 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 429
MspA1I CMGCKG 2 cut(s) 49, 731
MspI CCGG 7 cut(s) 21, 174, 288, 292, 300, 314, 360
MspR9I CCNGG 3 cut(s) 37, 175, 300
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 37
MwoI GCNNNNNNNGC 1 cut(s) 728
NaeI GCCGGC 1 cut(s) 289
NciI CCSGG 2 cut(s) 175, 300
NdeII GATC 3 cut(s) 191, 448, 465
NgoMIV GCCGGC 1 cut(s) 287
NlaIII CATG 3 cut(s) 101, 371, 710
NlaIV GGNNCC 5 cut(s) 383, 432, 607, 699, 737
NsiI ATGCAT 1 cut(s) 601
PdiI GCCGGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 295
PflMI CCANNNNNTGG 1 cut(s) 73
PfoI TCCNGGA 2 cut(s) 35, 173
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 2 cut(s) 51, 730
PleI GAGTC 3 cut(s) 34, 659, 809
PpsI GAGTC 3 cut(s) 34, 659, 809
Psp6I CCWGG 1 cut(s) 35
PspFI CCCAGC 1 cut(s) 731
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 5 cut(s) 383, 432, 607, 699, 737
PspPI GGNCC 1 cut(s) 605
PsrI GAACNNNNNNTAC 2 cut(s) 728, 760
PstI CTGCAG 2 cut(s) 211, 730
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 460, 660, 746
RsaNI GTAC 3 cut(s) 459, 659, 745
SatI GCNGC 2 cut(s) 50, 729
Sau3AI GATC 3 cut(s) 191, 448, 465
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 3 cut(s) 34, 659, 809
ScrFI CCNGG 3 cut(s) 37, 175, 300
SduI GDGCHC 1 cut(s) 386
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 2 cut(s) 207, 726
SinI GGWCC 1 cut(s) 605
SmlI CTYRAG 3 cut(s) 124, 231, 414
SmoI CTYRAG 3 cut(s) 124, 231, 414
Sse9I AATT 7 cut(s) 57, 70, 103, 166, 541, 561, 570
SsiI CCGC 4 cut(s) 49, 446, 456, 739
SspMI CTAG 2 cut(s) 284, 452
StyD4I CCNGG 3 cut(s) 35, 173, 298
TaaI ACNGT 2 cut(s) 589, 655
TaiI ACGT 1 cut(s) 580
TaqI TCGA 2 cut(s) 131, 468
TasI AATT 7 cut(s) 57, 70, 103, 166, 541, 561, 570
TatI WGTACW 2 cut(s) 658, 744
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 295
TscAI CASTG 2 cut(s) 201, 270
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 2 cut(s) 78, 333
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 2 cut(s) 201, 270
Van91I CCANNNNNTGG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 2 cut(s) 284, 452
ZraI GACGTC 1 cut(s) 578
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.