MD05G1026500.v1.1

Ankyrin repeat domain-containing protein EMB506

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011420
Physical Location & Seq
Forward (+)
4223362 .. 4226305
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1026500.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGGCACGGTTTCAATTTCCGCATTGTCGGGTCTAAATTCAATTTCCTTCCCCAGAGATTACAACCCGTTTGTAATTTTTAATAAAGCCGGCGTCTTGACAGCGTGTAGTACAAGGAGTGGAAGCTTTAGGTTCCATGGCGCTCAGAGGCACTCAAAGCTGAGGACTTTTTCGGTCGGTTCGTTGAGCCCGACTGCTTCGTTTCGGACCCAGGTGGGAACTTGGGAAGCACCCGACGATGGCAGCGGCAGCGAAGACGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAATTGGGTTTCCAAAGTGAATGGGAGGAAGAAGAGGGAAAAGGTGTTCCAGCTGCTGCTACTGATGCTGCTGCTGCTGTTGATAAGTCCACAACAAACAAGTATGAAGAGGATCTTGTAAAAGAAGTTGAACAGCTCTTGGGGCAAGAAGAAATAGCAATTTTGCAAAAGCATGCGGCTCCCAACATCAAGAAAATATCAACGGAAAAATGGAACCCGATACATACACTTGCACTGTCAGGGATGGTAACTTTCATGGACAAGCTACTTGAAGATGGTTTAGATATTGACGTTGTTGATAAGGATGGTACAACTGCTCTTCATACTGCAATCATAGCCAAAAAGGAAGCTGTAATTAGTCATCTGCTAAGAAAAGGTGCAAGTCCTCATGTCAGAGACAAGGATGGCGCTACGCCACTTCATTACGCAGTACATGTAGGCACCAAGCGGATTGTCAAGTTATTGATCAAATATAAAGTTGATGTCAATATTGCAGATAATGATGGATGGACTCCGTTGCATGTTGCTGTTCAAAGTAGAAACAGAGACATTGTGAAAATTTTGTTAGTCAATGGTGCAGATAAGAGCAGAAGAAATAAGGATGGCAGAACTCCGCTAGATCTGGGTTTATGTTATGGGAAAGATTTCAAGACCTATGACCTCACCAAGCTACTGAAGACGGTGCCAATGGACAGAGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

331

Amino Acids

36.39

Weight (kDa)

5.47

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 153 - 229 2.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 213 - 294 3.6e-21 Ankyrin repeats (3 copies)
Ank_5 PF13857 218 - 272 2.7e-10 Ankyrin repeats (many copies)
Ank PF00023 231 - 263 2.7e-07 Ankyrin repeat
Ank_4 PF13637 246 - 285 7.9e-08 Ankyrin repeats (many copies)
Ank PF00023 264 - 296 6.8e-08 Ankyrin repeat
Ank_5 PF13857 265 - 304 3.8e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40160 AT5G40160
fragaria_vesca FvH4_5g31250 FvH4_5g31250 FvH4_5g31250
malus_domestica MD05G1026500.v1.1
prunus_persica Prupe.3G132600_v2.0.a1
pyrus_communis pycom05g01830
rosa_chinensis RchiOBHm_Chr7g0226491
rosa_laevigata RLG00000001726
rosa_multiflora Rmu_sc0001423.1_g000067 Rmu_sc0001470.1_g000013
rosa_rugosa Rorug07G0237500
rosa_samantha Rh4CG052600 Rh7AG384700 Rh7BG371500 Rh7CG404000 Rh7DG383700
rosa_wichuraiana Rw4G003890 Rw7G032310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 728, 970
AciI CCGC 5 cut(s) 21, 246, 464, 736, 902
AclWI GGATC 1 cut(s) 408
AcsI RAATTY 2 cut(s) 37, 846
AcuI CTGAAG 1 cut(s) 983
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 112, 598, 720
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 6 cut(s) 15, 42, 419, 560, 821, 937
AjnI CCWGG 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 7 cut(s) 126, 160, 341, 424, 553, 638, 958
AluI AGCT 7 cut(s) 126, 160, 341, 424, 553, 638, 958
Alw26I GTCTC 2 cut(s) 678, 828
AlwI GGATC 1 cut(s) 408
AlwNI CAGNNNCTG 1 cut(s) 344
ApeKI GCWGC 7 cut(s) 243, 249, 341, 344, 356, 359, 362
ApoI RAATTY 2 cut(s) 37, 846
Asp700I GAANNNNTTC 1 cut(s) 932
AspLEI GCGC 2 cut(s) 143, 698
AspS9I GGNCC 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 943
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 728, 970
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 261, 971
BbvCI CCTCAGC 1 cut(s) 161
BbvI GCAGC 7 cut(s) 255, 261, 328, 331, 343, 346, 349
BccI CCATC 8 cut(s) 233, 526, 557, 587, 686, 785, 789, 884
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 1 cut(s) 753
BcoDI GTCTC 2 cut(s) 678, 828
BfaI CTAG 1 cut(s) 905
BfoI RGCGCY 2 cut(s) 144, 699
BglII AGATCT 1 cut(s) 907
BisI GCNGC 9 cut(s) 244, 247, 250, 342, 345, 357, 360, 363, 465
BlsI GCNGC 9 cut(s) 245, 248, 251, 343, 346, 358, 361, 364, 466
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 6 cut(s) 134, 209, 468, 503, 730, 972
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 2 cut(s) 261, 971
Bpu10I CCTNAGC 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 136, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 89
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 136, 210
BseGI GGATG 5 cut(s) 537, 598, 697, 800, 895
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 2 cut(s) 152, 158
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 7 cut(s) 255, 261, 328, 331, 343, 346, 349
BsgI GTGCAG 1 cut(s) 885
Bsh1285I CGRYCG 1 cut(s) 177
BshNI GGYRCC 2 cut(s) 728, 970
BsiEI CGRYCG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 678, 828
Bsp1286I GDGCHC 2 cut(s) 8, 191
Bsp143I GATC 3 cut(s) 400, 753, 907
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 5 cut(s) 21, 246, 464, 736, 902
BspCNI CTCAG 2 cut(s) 153, 157
BspLI GGNNCC 6 cut(s) 134, 209, 468, 503, 730, 972
BspPI GGATC 1 cut(s) 408
BspQI GCTCTTC 1 cut(s) 612
BspT107I GGYRCC 2 cut(s) 728, 970
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 136, 210
BssMI GATC 3 cut(s) 400, 753, 907
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 10, 525, 970
Bst6I CTCTTC 3 cut(s) 315, 390, 612
BstACI GRCGYC 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 91, 462
BstDEI CTNAG 3 cut(s) 144, 161, 656
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 5 cut(s) 537, 598, 697, 800, 895
BstH2I RGCGCY 2 cut(s) 144, 699
BstHHI GCGC 2 cut(s) 143, 698
BstKTI GATC 3 cut(s) 403, 756, 910
BstMAI GTCTC 2 cut(s) 678, 828
BstMBI GATC 3 cut(s) 400, 753, 907
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 6 cut(s) 157, 249, 353, 362, 430, 623
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 464, 725, 812
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 7 cut(s) 255, 261, 328, 331, 343, 346, 349
BstV2I GAAGAC 2 cut(s) 261, 971
BstX2I RGATCY 2 cut(s) 400, 907
BstYI RGATCY 2 cut(s) 400, 907
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 5 cut(s) 537, 598, 697, 800, 895
BtsIMutI CAGTG 1 cut(s) 521
Cac8I GCNNGC 2 cut(s) 91, 462
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 2 cut(s) 143, 698
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 82
Csp6I GTAC 3 cut(s) 111, 597, 719
CviAII CATG 6 cut(s) 137, 461, 544, 677, 722, 809
CviQI GTAC 3 cut(s) 111, 597, 719
DdeI CTNAG 3 cut(s) 144, 161, 656
DpnI GATC 3 cut(s) 402, 755, 909
DpnII GATC 3 cut(s) 400, 753, 907
Eam1104I CTCTTC 3 cut(s) 315, 390, 612
EarI CTCTTC 3 cut(s) 315, 390, 612
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 983
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 6 cut(s) 140, 464, 547, 680, 725, 812
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FatI CATG 6 cut(s) 136, 460, 543, 676, 721, 808
FbaI TGATCA 1 cut(s) 753
Fnu4HI GCNGC 9 cut(s) 244, 247, 250, 342, 345, 357, 360, 363, 465
FokI GGATG 5 cut(s) 544, 605, 704, 807, 902
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 9 cut(s) 244, 247, 250, 342, 345, 357, 360, 363, 465
FspBI CTAG 1 cut(s) 905
GlaI GCGC 2 cut(s) 142, 697
GluI GCNGC 9 cut(s) 244, 247, 250, 342, 345, 357, 360, 363, 465
HaeII RGCGCY 2 cut(s) 144, 699
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 82
HhaI GCGC 2 cut(s) 143, 698
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 6 cut(s) 140, 464, 547, 680, 725, 812
Hin6I GCGC 2 cut(s) 141, 696
HinP1I GCGC 2 cut(s) 141, 696
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 1 cut(s) 799
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 943
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 3 cut(s) 147, 207, 683
Hpy188III TCNNGA 3 cut(s) 97, 478, 937
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 3 cut(s) 10, 525, 970
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 7 cut(s) 454, 521, 617, 668, 782, 808, 866
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 249, 353, 362, 430, 623
HpyF3I CTNAG 3 cut(s) 144, 161, 656
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 6 cut(s) 140, 464, 547, 680, 725, 812
HspAI GCGC 2 cut(s) 141, 696
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 753
Kzo9I GATC 3 cut(s) 400, 753, 907
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 1 cut(s) 472
LpnPI CCDG 7 cut(s) 67, 103, 197, 224, 351, 513, 896
Lsp1109I GCAGC 7 cut(s) 255, 261, 328, 331, 343, 346, 349
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 905
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 535
MalI GATC 3 cut(s) 402, 755, 909
MboI GATC 3 cut(s) 400, 753, 907
MflI RGATCY 2 cut(s) 400, 907
MhlI GDGCHC 2 cut(s) 8, 191
MluCI AATT 8 cut(s) 15, 37, 42, 75, 290, 447, 642, 846
MlyI GAGTC 1 cut(s) 793
MnlI CCTC 9 cut(s) 141, 156, 253, 256, 307, 316, 391, 684, 959
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 932
MseI TTAA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 246, 341
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 6 cut(s) 157, 249, 353, 362, 430, 623
NaeI GCCGGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 136
NdeII GATC 3 cut(s) 400, 753, 907
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 6 cut(s) 140, 464, 547, 680, 725, 812
NlaIV GGNNCC 6 cut(s) 134, 209, 468, 503, 730, 972
NspI RCATGY 3 cut(s) 464, 725, 812
PaeI GCATGC 1 cut(s) 464
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 188
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 932
PkrI GCNGC 9 cut(s) 245, 248, 251, 343, 346, 358, 361, 364, 466
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
PscI ACATGT 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 6 cut(s) 134, 209, 468, 503, 730, 972
PspPI GGNCC 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 2 cut(s) 400, 907
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 3 cut(s) 112, 598, 720
RsaNI GTAC 3 cut(s) 111, 597, 719
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 9 cut(s) 244, 247, 250, 342, 345, 357, 360, 363, 465
Sau3AI GATC 3 cut(s) 400, 753, 907
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 8, 191
SfaNI GCATC 1 cut(s) 343
SinI GGWCC 1 cut(s) 207
SphI GCATGC 1 cut(s) 464
Sse9I AATT 8 cut(s) 15, 37, 42, 75, 290, 447, 642, 846
SsiI CCGC 5 cut(s) 21, 246, 464, 736, 902
SspI AATATT 1 cut(s) 778
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 10, 525, 970
TaiI ACGT 1 cut(s) 582
TaqII GACCGA 1 cut(s) 163
TasI AATT 8 cut(s) 15, 37, 42, 75, 290, 447, 642, 846
TatI WGTACW 2 cut(s) 110, 718
TauI GCSGC 2 cut(s) 249, 467
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 528
TseI GCWGC 7 cut(s) 243, 249, 341, 344, 356, 359, 362
TspDTI ATGAA 4 cut(s) 408, 532, 599, 698
TspGWI ACGGA 2 cut(s) 506, 792
TspRI CASTG 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 2 cut(s) 37, 846
XceI RCATGY 3 cut(s) 464, 725, 812
XmnI GAANNNNTTC 1 cut(s) 932
XspI CTAG 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.