Rmu_sc0001423.1_g000067

Ankyrin repeat domain-containing protein EMB506

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001423.1
OG0011420
Physical Location & Seq
Forward (+)
294549 .. 296494
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001423.1_g000067.1.cds

Sequence Viewer

Length: 1017 bp
atggtttcaataccagcattctcagctgtacagtcagtctctatacccagagattatcattcgtttgcggttgtgaactctttgaaggccaaggggaagagttgtcaactagaattgcatagtaataccagtgtcttgaaagcatatagtatatggagtggtggcagctttagctcctctgacaagcattcaaagttgaggacctttttggtcgagtcaaaccaagcatcttcgttacagtctcgtgtgggaacatgggaaggtcctgatgatggcagtgggactgaatctgaggaggaggaggaggaagaagaagaacttgacttgggtttccaaagcgaatgggaagaagatgaagaaaagcgggctccagctgctaatgctggtgatgttcagaagtccacaacaaatgagttagaagagcaacttgtgaaagaggtggaacagctcttggaggtagaagaaagagcaattttgcaaaagcatgcagctcccaacatggagaaattatcaactgcaaaatggagtccactacatactctttcactatctgggctagttactttcatggataagctacttgaagatggtgtagatattaatcgttttgataatgatggtgtaaccgctcttcatactgcaatcataggcaaaaaagaagctgttattagtcatcttctaagaaaaggtgcaagtcctcatgtcagagatcgggatggcgctacaccacttcattatgcagttcacgtaggcaccaagcgaattgtgaagttattgatcaaatacaaagttgatgtcaacattgcagataatgatggatggacgccattgcatgttgccgtgcaaagtagaaatagagatattgtgaaacttttgttagtgaatggtgcagataagagtagaagaaataaggatggcagaactcccatagatctgggcttgtgttatgggaaagacttcaagtcctatgacctcaccaagttactgaagacagtaccaatggacagagatttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

338

Amino Acids

37.55

Weight (kDa)

5.54

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40160 AT5G40160
fragaria_vesca FvH4_5g31250 FvH4_5g31250 FvH4_5g31250
malus_domestica MD05G1026500.v1.1
prunus_persica Prupe.3G132600_v2.0.a1
pyrus_communis pycom05g01830
rosa_chinensis RchiOBHm_Chr7g0226491
rosa_laevigata RLG00000001726
rosa_multiflora Rmu_sc0001423.1_g000067 Rmu_sc0001470.1_g000013
rosa_rugosa Rorug07G0237500
rosa_samantha Rh4CG052600 Rh7AG384700 Rh7BG371500 Rh7CG404000 Rh7DG383700
rosa_wichuraiana Rw4G003890 Rw7G032310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 752
AccBSI CCGCTC 1 cut(s) 629
AciI CCGC 3 cut(s) 68, 364, 627
AcuI CTGAAG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 824
AfaI GTAC 2 cut(s) 30, 996
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 6 cut(s) 9, 85, 139, 192, 584, 961
AhdI GACNNNNNGTC 1 cut(s) 961
AluBI AGCT 8 cut(s) 26, 168, 174, 374, 448, 491, 577, 662
AluI AGCT 8 cut(s) 26, 168, 174, 374, 448, 491, 577, 662
Alw26I GTCTC 2 cut(s) 43, 246
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 3 cut(s) 165, 374, 488
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 1 cut(s) 956
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 2 cut(s) 201, 263
AsuHPI GGTGA 2 cut(s) 398, 967
AvaII GGWCC 2 cut(s) 201, 263
BanI GGYRCC 1 cut(s) 752
BanII GRGCYC 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BauI CACGAG 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 995
BbvI GCAGC 3 cut(s) 177, 361, 500
BccI CCATC 7 cut(s) 266, 581, 611, 710, 809, 813, 908
BceAI ACGGC 1 cut(s) 824
BclI TGATCA 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 43, 246
BfaI CTAG 2 cut(s) 110, 557
BfoI RGCGCY 1 cut(s) 723
BglII AGATCT 1 cut(s) 931
BisI GCNGC 3 cut(s) 166, 375, 489
BlsI GCNGC 3 cut(s) 167, 376, 490
Bme18I GGWCC 2 cut(s) 201, 263
BmeRI GACNNNNNGTC 1 cut(s) 961
BmgT120I GGNCC 2 cut(s) 201, 263
BmiI GGNNCC 2 cut(s) 369, 754
BmsI GCATC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 995
BpmI CTGGAG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 824
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 129
Bse3DI GCAATG 2 cut(s) 801, 827
Bse8I GATNNNNATC 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 3 cut(s) 721, 824, 919
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMI GCAATG 2 cut(s) 801, 827
BseMII CTCAG 2 cut(s) 36, 282
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 5 cut(s) 166, 308, 311, 314, 317
BseXI GCAGC 3 cut(s) 177, 361, 500
BsgI GTGCAG 1 cut(s) 909
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 752
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 2 cut(s) 43, 246
BsmFI GGGAC 1 cut(s) 295
BsmI GAATGC 2 cut(s) 17, 187
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 28
Bsp143I GATC 3 cut(s) 709, 777, 931
BspACI CCGC 3 cut(s) 68, 364, 627
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 2 cut(s) 35, 283
BspLI GGNNCC 2 cut(s) 369, 754
BspQI GCTCTTC 2 cut(s) 414, 636
BspT107I GGYRCC 1 cut(s) 752
BsrBI CCGCTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 801, 827
BsrGI TGTACA 1 cut(s) 28
BsrI ACTGG 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 3 cut(s) 709, 777, 931
BssNI GRCGYC 1 cut(s) 824
BssSI CACGAG 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 243
Bst4CI ACNGT 3 cut(s) 33, 240, 994
Bst6I CTCTTC 3 cut(s) 92, 414, 636
BstACI GRCGYC 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 748
BstC8I GCNNGC 2 cut(s) 366, 486
BstDEI CTNAG 3 cut(s) 22, 291, 680
BstF5I GGATG 3 cut(s) 721, 824, 919
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 3 cut(s) 712, 780, 934
BstMAI GTCTC 2 cut(s) 43, 246
BstMBI GATC 3 cut(s) 709, 777, 931
BstMWI GCNNNNNNNGC 4 cut(s) 23, 171, 374, 380
BstNSI RCATGY 2 cut(s) 488, 836
BstV1I GCAGC 3 cut(s) 177, 361, 500
BstV2I GAAGAC 1 cut(s) 995
BstX2I RGATCY 1 cut(s) 931
BstXI CCANNNNNNTGG 1 cut(s) 934
BstYI RGATCY 1 cut(s) 931
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 3 cut(s) 721, 824, 919
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 136, 283
Cac8I GCNNGC 2 cut(s) 366, 486
CfoI GCGC 1 cut(s) 722
Cfr13I GGNCC 2 cut(s) 201, 263
CseI GACGC 1 cut(s) 832
Csp6I GTAC 2 cut(s) 29, 995
CviAII CATG 6 cut(s) 255, 485, 499, 568, 701, 833
CviQI GTAC 2 cut(s) 29, 995
DdeI CTNAG 3 cut(s) 22, 291, 680
DpnI GATC 3 cut(s) 711, 779, 933
DpnII GATC 3 cut(s) 709, 777, 931
DriI GACNNNNNGTC 1 cut(s) 961
Eam1104I CTCTTC 3 cut(s) 92, 414, 636
Eam1105I GACNNNNNGTC 1 cut(s) 961
EarI CTCTTC 3 cut(s) 92, 414, 636
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 370
Eco47I GGWCC 2 cut(s) 201, 263
Eco57I CTGAAG 1 cut(s) 1007
EcoO109I RGGNCCY 2 cut(s) 201, 263
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 6 cut(s) 258, 488, 502, 571, 704, 836
FalI AAGNNNNNCTT 4 cut(s) 303, 335, 411, 443
FaqI GGGAC 1 cut(s) 295
FatI CATG 6 cut(s) 254, 484, 498, 567, 700, 832
FauI CCCGC 1 cut(s) 357
FbaI TGATCA 1 cut(s) 777
Fnu4HI GCNGC 3 cut(s) 166, 375, 489
FokI GGATG 3 cut(s) 728, 831, 926
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 3 cut(s) 166, 375, 489
FspBI CTAG 2 cut(s) 110, 557
GlaI GCGC 1 cut(s) 721
GluI GCNGC 3 cut(s) 166, 375, 489
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 1 cut(s) 89
HgaI GACGC 1 cut(s) 832
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 824
Hin1II CATG 6 cut(s) 258, 488, 502, 571, 704, 836
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HincII GTYRAC 2 cut(s) 107, 799
HindII GTYRAC 2 cut(s) 107, 799
HinfI GANTC 3 cut(s) 215, 287, 526
HphI GGTGA 2 cut(s) 398, 967
Hpy166II GTNNAC 6 cut(s) 76, 107, 402, 530, 745, 799
Hpy188I TCNGA 4 cut(s) 181, 292, 396, 707
Hpy188III TCNNGA 3 cut(s) 136, 266, 713
Hpy8I GTNNAC 6 cut(s) 76, 107, 402, 530, 745, 799
HpyAV CCTTC 2 cut(s) 79, 254
HpyCH4III ACNGT 3 cut(s) 33, 240, 994
HpyCH4IV ACGT 1 cut(s) 747
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 171, 374, 380
HpyF3I CTNAG 3 cut(s) 22, 291, 680
HpySE526I ACGT 1 cut(s) 747
Hsp92I GRCGYC 1 cut(s) 824
Hsp92II CATG 6 cut(s) 258, 488, 502, 571, 704, 836
HspAI GCGC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 777
Kzo9I GATC 3 cut(s) 709, 777, 931
LguI GCTCTTC 2 cut(s) 414, 636
LmnI GCTCC 3 cut(s) 179, 373, 496
LpnPI CCDG 8 cut(s) 27, 61, 142, 279, 369, 384, 537, 920
Lsp1109I GCAGC 3 cut(s) 177, 361, 500
LweI GCATC 1 cut(s) 236
MaeI CTAG 2 cut(s) 110, 557
MaeII ACGT 1 cut(s) 747
MaeIII GTNAC 4 cut(s) 234, 559, 622, 981
MalI GATC 3 cut(s) 711, 779, 933
MbiI CCGCTC 1 cut(s) 629
MboI GATC 3 cut(s) 709, 777, 931
MflI RGATCY 1 cut(s) 931
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 4 cut(s) 113, 471, 506, 762
MlyI GAGTC 2 cut(s) 224, 535
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 26, 374
Mva1269I GAATGC 2 cut(s) 17, 187
MwoI GCNNNNNNNGC 4 cut(s) 23, 171, 374, 380
NdeII GATC 3 cut(s) 709, 777, 931
NlaIII CATG 6 cut(s) 258, 488, 502, 571, 704, 836
NlaIV GGNNCC 2 cut(s) 369, 754
NspI RCATGY 2 cut(s) 488, 836
PaeI GCATGC 1 cut(s) 488
PciSI GCTCTTC 2 cut(s) 414, 636
PctI GAATGC 2 cut(s) 17, 187
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 3 cut(s) 167, 376, 490
PleI GAGTC 2 cut(s) 223, 534
PpsI GAGTC 2 cut(s) 223, 534
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 2 cut(s) 201, 263
PshBI ATTAAT 1 cut(s) 600
Psp5II RGGWCCY 2 cut(s) 201, 263
PspN4I GGNNCC 2 cut(s) 369, 754
PspPI GGNCC 2 cut(s) 201, 263
PspPPI RGGWCCY 2 cut(s) 201, 263
PsuI RGATCY 1 cut(s) 931
PvuII CAGCTG 2 cut(s) 26, 374
RsaI GTAC 2 cut(s) 30, 996
RsaNI GTAC 2 cut(s) 29, 995
SapI GCTCTTC 2 cut(s) 414, 636
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 3 cut(s) 166, 375, 489
Sau3AI GATC 3 cut(s) 709, 777, 931
Sau96I GGNCC 2 cut(s) 201, 263
SchI GAGTC 2 cut(s) 224, 535
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 1 cut(s) 236
SinI GGWCC 2 cut(s) 201, 263
SphI GCATGC 1 cut(s) 488
Sse9I AATT 4 cut(s) 113, 471, 506, 762
SsiI CCGC 3 cut(s) 68, 364, 627
SspMI CTAG 2 cut(s) 110, 557
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 3 cut(s) 33, 240, 994
TaiI ACGT 1 cut(s) 750
TaqI TCGA 1 cut(s) 213
TasI AATT 4 cut(s) 113, 471, 506, 762
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 2 cut(s) 136, 283
TseI GCWGC 3 cut(s) 165, 374, 488
TspDTI ATGAA 4 cut(s) 369, 556, 623, 722
TspRI CASTG 2 cut(s) 136, 283
VpaK11BI GGWCC 2 cut(s) 201, 263
VspI ATTAAT 1 cut(s) 600
XceI RCATGY 2 cut(s) 488, 836
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 2 cut(s) 110, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.