Rh7BG371500

Ankyrin repeat domain-containing protein EMB506

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011420
Physical Location & Seq
Reverse (-)
39912541 .. 39916029
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG371500.1

Sequence Viewer

Length: 741 bp
ATGGGCATGGTTTCAATACCAGCATTCTCAGCTGTACAGTCAGTCTCTATACCCAGAGATTATCATTCGTTTGCGATTGTGAACTCTTTGAAGGCCAAGGGGAAGAGTCATCAATTAGAATTGCTTGGTAACACCAATGTCTTGAAAGCATGTAGTATATGGAGTGGTGGCAGCTTTAGCTCCTCTGATAAGCACTCAAAGTTGAGGACCTTTTTGGTCGAGTCAAACCAAGCATCTTCGTTACAATCTCGTGTGGGAACATGGGAAGGTCCTGAATCTGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAACATGACTTGGGTTTCCAAAGCGAATGGGAAGAAGATGAAGAAAAGCGGGCTCCAGCTGCTAATGCTCGTGATGTTCAGAAGCTCACAACAAATGAGTTAGAAGAGCAACTTGTTAAAGAGGTGGAACAGCTCTTGGAGGTAGAAGAAAGAGCAATTTTGCAAAAGCATGCAGCTCCCAACATGGAGAAATTATCAACTGCAAAATGGAGCCCACTACATACTCTTTCACTATCTGGGCTAGTTACTTTCATGGATAAGCTGCTTGAAGATGGTGTAGATATTAATCTTTTTGATAAAGATGGTGTAACCGCTCTTCACACTGCAATCATAGGCAAAAAAGAAGCTGTTATTAGTCATCTTCTAAGAAAAGGTGCAAGTCCTCATGTCAGAGATCGGGTAGGAAGATATTTCACCAACATCTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

246

Amino Acids

27.4

Weight (kDa)

5.07

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 159 - 235 2.8e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40160 AT5G40160
fragaria_vesca FvH4_5g31250 FvH4_5g31250 FvH4_5g31250
malus_domestica MD05G1026500.v1.1
prunus_persica Prupe.3G132600_v2.0.a1
pyrus_communis pycom05g01830
rosa_chinensis RchiOBHm_Chr7g0226491
rosa_laevigata RLG00000001726
rosa_multiflora Rmu_sc0001423.1_g000067 Rmu_sc0001470.1_g000013
rosa_rugosa Rorug07G0237500
rosa_samantha Rh4CG052600 Rh7AG384700 Rh7BG371500 Rh7CG404000 Rh7DG383700
rosa_wichuraiana Rw4G003890 Rw7G032310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 623
AciI CCGC 2 cut(s) 358, 621
AfaI GTAC 1 cut(s) 36
AgsI TTSAA 4 cut(s) 15, 91, 145, 578
AluBI AGCT 9 cut(s) 32, 174, 180, 368, 394, 442, 485, 571, 656
AluI AGCT 9 cut(s) 32, 174, 180, 368, 394, 442, 485, 571, 656
Alw26I GTCTC 1 cut(s) 49
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 4 cut(s) 171, 368, 482, 571
AseI ATTAAT 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 207, 269
AsuHPI GGTGA 1 cut(s) 715
AvaII GGWCC 2 cut(s) 207, 269
BanII GRGCYC 2 cut(s) 364, 524
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BauI CACGAG 2 cut(s) 249, 378
BbvI GCAGC 4 cut(s) 183, 355, 494, 558
BccI CCATC 2 cut(s) 575, 605
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 1 cut(s) 551
BisI GCNGC 4 cut(s) 172, 369, 483, 572
BlsI GCNGC 4 cut(s) 173, 370, 484, 573
Bme18I GGWCC 2 cut(s) 207, 269
BmgT120I GGNCC 2 cut(s) 207, 269
BmiI GGNNCC 2 cut(s) 363, 521
BmsI GCATC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 96
BseJI GATNNNNATC 1 cut(s) 594
BseMII CTCAG 2 cut(s) 42, 270
BseRI GAGGAG 6 cut(s) 172, 296, 299, 302, 305, 308
BseXI GCAGC 4 cut(s) 183, 355, 494, 558
BshFI GGCC 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 364, 524
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 1 cut(s) 703
BspACI CCGC 2 cut(s) 358, 621
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 2 cut(s) 41, 271
BspLI GGNNCC 2 cut(s) 363, 521
BspQI GCTCTTC 2 cut(s) 408, 630
BsrBI CCGCTC 1 cut(s) 623
BsrGI TGTACA 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 1 cut(s) 703
BssSI CACGAG 2 cut(s) 249, 378
BssT1I CCWWGG 1 cut(s) 96
Bst2BI CACGAG 2 cut(s) 249, 378
Bst4CI ACNGT 1 cut(s) 39
Bst6I CTCTTC 3 cut(s) 98, 408, 630
BstAUI TGTACA 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 360, 480
BstDEI CTNAG 3 cut(s) 28, 279, 674
BstKTI GATC 1 cut(s) 706
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 1 cut(s) 703
BstMWI GCNNNNNNNGC 4 cut(s) 29, 177, 368, 374
BstNSI RCATGY 2 cut(s) 153, 482
BstV1I GCAGC 4 cut(s) 183, 355, 494, 558
BsuRI GGCC 1 cut(s) 95
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 1 cut(s) 630
Cac8I GCNNGC 2 cut(s) 360, 480
Cfr13I GGNCC 2 cut(s) 207, 269
Csp6I GTAC 1 cut(s) 35
CviAII CATG 8 cut(s) 7, 150, 261, 314, 479, 493, 562, 695
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 3 cut(s) 28, 279, 674
DpnI GATC 1 cut(s) 705
DpnII GATC 1 cut(s) 703
Eam1104I CTCTTC 3 cut(s) 98, 408, 630
EarI CTCTTC 3 cut(s) 98, 408, 630
Eco130I CCWWGG 1 cut(s) 96
Eco24I GRGCYC 2 cut(s) 364, 524
Eco47I GGWCC 2 cut(s) 207, 269
EcoO109I RGGNCCY 2 cut(s) 207, 269
EcoT14I CCWWGG 1 cut(s) 96
EcoT38I GRGCYC 2 cut(s) 364, 524
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 8 cut(s) 10, 153, 264, 317, 482, 496, 565, 698
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 8 cut(s) 6, 149, 260, 313, 478, 492, 561, 694
FauI CCCGC 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 172, 369, 483, 572
FriOI GRGCYC 2 cut(s) 364, 524
Fsp4HI GCNGC 4 cut(s) 172, 369, 483, 572
FspBI CTAG 1 cut(s) 551
GluI GCNGC 4 cut(s) 172, 369, 483, 572
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 95
Hin1II CATG 8 cut(s) 10, 153, 264, 317, 482, 496, 565, 698
HinfI GANTC 3 cut(s) 106, 221, 275
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 4 cut(s) 187, 280, 390, 701
Hpy188III TCNNGA 3 cut(s) 142, 272, 380
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 85, 260
HpyCH4III ACNGT 1 cut(s) 39
HpyCH4V TGCA 5 cut(s) 472, 482, 512, 635, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 177, 368, 374
HpyF3I CTNAG 3 cut(s) 28, 279, 674
Hsp92II CATG 8 cut(s) 10, 153, 264, 317, 482, 496, 565, 698
Kzo9I GATC 1 cut(s) 703
LguI GCTCTTC 2 cut(s) 408, 630
LmnI GCTCC 4 cut(s) 185, 367, 490, 519
LpnPI CCDG 5 cut(s) 33, 67, 285, 378, 531
Lsp1109I GCAGC 4 cut(s) 183, 355, 494, 558
LweI GCATC 1 cut(s) 242
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 4 cut(s) 128, 240, 553, 616
MalI GATC 1 cut(s) 705
MbiI CCGCTC 1 cut(s) 623
MboI GATC 1 cut(s) 703
MhlI GDGCHC 2 cut(s) 364, 524
MluCI AATT 4 cut(s) 113, 119, 465, 500
MlyI GAGTC 2 cut(s) 115, 230
MseI TTAA 2 cut(s) 426, 594
MspA1I CMGCKG 2 cut(s) 32, 368
Mva1269I GAATGC 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 29, 177, 368, 374
NdeII GATC 1 cut(s) 703
NlaIII CATG 8 cut(s) 10, 153, 264, 317, 482, 496, 565, 698
NlaIV GGNNCC 2 cut(s) 363, 521
NspI RCATGY 2 cut(s) 153, 482
PaeI GCATGC 1 cut(s) 482
PciSI GCTCTTC 2 cut(s) 408, 630
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 4 cut(s) 173, 370, 484, 573
PleI GAGTC 2 cut(s) 114, 229
PpsI GAGTC 2 cut(s) 114, 229
PpuMI RGGWCCY 2 cut(s) 207, 269
PshBI ATTAAT 1 cut(s) 594
Psp5II RGGWCCY 2 cut(s) 207, 269
PspN4I GGNNCC 2 cut(s) 363, 521
PspPI GGNCC 2 cut(s) 207, 269
PspPPI RGGWCCY 2 cut(s) 207, 269
PvuII CAGCTG 2 cut(s) 32, 368
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SapI GCTCTTC 2 cut(s) 408, 630
SaqAI TTAA 2 cut(s) 426, 594
SatI GCNGC 4 cut(s) 172, 369, 483, 572
Sau3AI GATC 1 cut(s) 703
Sau96I GGNCC 2 cut(s) 207, 269
SchI GAGTC 2 cut(s) 115, 230
SduI GDGCHC 2 cut(s) 364, 524
SfaNI GCATC 1 cut(s) 242
SinI GGWCC 2 cut(s) 207, 269
SphI GCATGC 1 cut(s) 482
Sse9I AATT 4 cut(s) 113, 119, 465, 500
SsiI CCGC 2 cut(s) 358, 621
SspMI CTAG 1 cut(s) 551
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 219
TasI AATT 4 cut(s) 113, 119, 465, 500
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 426, 594
Tru9I TTAA 2 cut(s) 426, 594
TscAI CASTG 1 cut(s) 637
TseI GCWGC 4 cut(s) 171, 368, 482, 571
TspDTI ATGAA 2 cut(s) 363, 550
TspRI CASTG 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 207, 269
VspI ATTAAT 1 cut(s) 594
XceI RCATGY 2 cut(s) 153, 482
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.