Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005040

Physical Location & Seq

Genomic Coordinates Reverse (-)

4539169 .. 4550588

Gene Structure 11420 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1028000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGAGGAAGAAGGTGGACGAACGAATCCGAACCCTAATAAGCAATGGCGTCAAAAACCGGCACCGCTCCATGTTCGTCATCGTCGGCGACAAATCCCGTGACCAGATTGTGAATCTTCATTATATGCTAAGCAATGAAGTAAAGAAATCGCGCCCGACTGTGTTGTGGTGCTACAAGGACAAGCTCGAGCTCAGCAGTCACAAGAAAAAACGTGCTAAGCAAGTAAAAAAGATGATGCAGAGGGGGCTGCTTGATCCCGAGAAGGTTGATCCTTTCTCACTTTTTGTTGAAAGTGGGGGATTGACTTATTGCTTGTACAAGGATTCAGAAAGAGTTCTTGGTAATACCTTTGGAATGTGTATACTTCAGGATTTTGAGGCCTTGACTCCAAATCTACTAGCAAGAACGATAGAGACAGTGGAGGGAGGTGGACTAGTCATATTGTTGCTTCATTCTCTAACCTCACTCACCAACCTCTACACCATGGTTATGGATGTTCATGATAGGTTTCGAACTGAATCCCATTCTAAGGCTACTGGACGTTTTAACGAGCGTTTCTTATTGTCACTTGCGTCATGCAAATCATGTGTAGTAATGGATGATGAGCTGAATATTTTACCAATTTCATCTCATATGAGGTCAATTACCCCAGTTCCAGTGAAAGAGGACTCTGAAGGGATATCAGAGTCACAAAGGGAACTGAAGGATCTAAAAGAGCAGCTGAGTGATGCCTTTCCTGTTGGCCCTTTAATAAAGAAATGTTGTACGTTGGACCAGGGAAATGCTGTTGCTACATTTCTTGATGCAATCTTGGACAAGACACTCCGAAGTACTGTTGCCTTGCTTGCTGCTCGAGGCCGTGGAAAATCGGCTGCACTTGGTTTGGCAATTTCCGGAGCTATTGCTGCAGGGTATTCAAATATATTTGTAACTGCACCCAGCCCTGAGAACTTAAGAACCTTGTTCGAATTTGTTTGCAAGGGTTTTGATCAACTGGACTATAAGGAACATATAGATTATGATGTACAGAAAAGTAGTGACCCTTTGTTGAAGAAAGCAACTGTACAGATCAATATATACAAGCAGCACAGACAAACAATTCAGTATATACGGCCACATGAGCATGAAAAGCTTTCCCAAGTTGAACTGTTGGTTGTTGATGAAGCAGCTGCTATTCCACTACCAGTAGTGAAGTCTTTGCTTGGTCCTTATCTGGTCTTCCTTTCATCTACTGTTAATGGCTATGAAGGTACTGGTCGGTCTTTGTCCCTAAAACTTCTACAGCAATTGGAAGAGCAAAGCCAAGTGTCTGCTAAGGGGCCTACATCTGGTCGTCTTTTCAAAAAGATAGAATTGAAAGAATCTATCAGATATGCTTCTGGTGATCCGATTGAATCCTGGCTTCATGGTTTACTTTGCTTGGATATCACAAATTATATCCCTAAACTTAACGGGTTACCTGCACCCAGTGAGTGCGATCTGTACTACGTAAACTGTGATACGCTTTTTTCATATCATAAAGATAGCGAGTTGTTCTTACAGCGGATGATGGCTCTATATGTTGCCTCCCACTACAAAAATTCTCCAAATGATTTGCAACTAATGGCTGATGCCCCGGCTCACCACCTGTTTGTGCTACTTGGCCCTGTTGATGAGTCAAAAAATCAGCTTCCTGATATTTTGTGTGTCATTCAGGTCTGCCTTGAGGGAAAGATCTCTCGTGAATCTGCAATGAAAAGTTTAAGTGATGGTCATCAGCCTTCTGGTGACCAGATACCATGGAAATTCTGTGAGCAATTTCAGGACACAGTTTTTCCCAGTCTTTCAGGTGCTCGGATTGTACGAATTGCTACACATCCAAGTGCCATGAAGATTGGATATGGATCACAAGCAGTGGAACTATTGACAAGGTACTACGAAGGACAGTTTGCTCCTATTTCTGAACTGGACGTTGAAGATGTTGAGACTCCCCCACTCAGAGTCACAGAAGCTGCTGAGAAGGTTTCATTGCTGGAAGAAAGTATAAAACCTAGAACAGATCTTCCTCATTTGCTTGTTCATCTTCGTGAAAGGCGACCAGAGAAGCTCCATTATATTGGTGTCTCCTTTGGGCTTACCTTGGACCTTTTCAGGTTTTGGTGGAAACATAAGTTTGCTCCATTCTATATTGGACATATTCCAAGTGGTGTGACCGGGGAGCATACGTGTATGGTCCTCAAATCTTTGAAGAATGATGAACTTGAAACCAGTGATTTTCGTCCATTCAACCTAGATTTCAGGAGAAGATTTTTGAGACTATTGGGTTATAGTTTTCGTACAATGGATTATAGGCTCGCTATGAGCATCTTAGATCCTAAGATCAATTACAAGGATGGAGAACCCAAATCGTCTACCACAGATGGATTCTTACGGTCTATCACAGACTTACTTTCGCCTTATGATATGAAGCGACTGGAGGCCTACACCAGCAGTCTTGCTGACTTTCATATGATTTTGGATCTTGTTCCAACGCTTTCACATCTGTATTTCCAAGAGAAACTTCCAGTGACATTGACAGGTGCCCAGGCTTCCATATTGCTCTGCATTGGCTTACAGAATCAAGATATCTCCTATATTGAGGGGTTGATGAAACTGGAAAGGCAGCAAATATTGTCTCTGTTTATAAAAGTTATGAAAAAGTTTCACAAATATCTATACGCTATTGCATCTGAGGGCCTTGTGTCAACCTTGCCTAAACCAAAAGAAACTCTCTTGGAACCTCACAAGATCTCTGTTGATGATGATCTCAATGATGCTGCAAGAAAAGTTGAGGATGGGATGAGATCCAACTCGGAGGGGTCATTAGATCCCAAGTTGCTTCAACAGTACGCGATTGGGGACAGAGATGCAGACTTTGAGAATGCATTACAGAATGGCGGTGCAAACCTACCTGCTGGTGGTCTCGTTAGCGTGAAATCAAGTAGAAACAAAATGGATAAAGGAAAACAGAGAGAGAGTCACAAGAGTGGGGAAAAGCGACATAAAAATGAGCATGGCTCCAACTCAAAGTCCAACAAAAAGAAGAAACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0000154 GO:0002097 GO:0002101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008080 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051391 GO:0051392 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1904812 GO:1990882 GO:1990883 GO:1990884 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DUF1726 GNAT_acetyltr_2 Helicase_RecD tRNA_bind_2

Protein Analysis

1024

Amino Acids

115.33

Weight (kDa)

8.41

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TmcA_N	PF08351	1 - 200	4.6e-20	tRNA(Met) cytidine acetyltransferase TmcA, N-terminal
NAT10_TcmA_helicase	PF05127	281 - 475	9.2e-56	RNA cytidine acetyltransferase NAT10/TcmA, helicase domain
GNAT_acetyltr_2	PF13718	515 - 743	8.4e-96	GNAT acetyltransferase 2
tRNA_bind_2	PF13725	753 - 968	5.6e-66	Possible tRNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005040)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10490 AT1G10490 AT3G57940 AT3G57940
fragaria_vesca	FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910
malus_domestica	MD05G1028000.v1.1 MD10G1028600.v1.1
prunus_persica	Prupe.6G169200_v2.0.a1
pyrus_communis	pycom05g02010 pycom10g01860
rosa_chinensis	RchiOBHm_Chr2g0117351
rosa_laevigata	RLG00000018344
rosa_multiflora	Rmu_co7970672.1_g000001 Rmu_sc0004436.1_g000001 Rmu_sc0013504.1_g000001
rosa_roxburghii	Rroxscaffold_2G00126550
rosa_rugosa	Rorug02G0212300 Rorug02G0212400
rosa_samantha	Rh2AG267400 Rh2BG279800 Rh2DG274500 Rh2DG293800
rosa_wichuraiana	Rw2G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2663
Acc36I	ACCTGC	2 cut(s)	1468, 2938
AccB1I	GGYRCC	2 cut(s)	60, 2558
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	2 cut(s)	361, 2390
AccII	CGCG	2 cut(s)	151, 2870
AccIII	TCCGGA	1 cut(s)	893
AciI	CCGC	3 cut(s)	64, 1543, 2916
AclWI	GGATC	9 cut(s)	248, 263, 714, 1379, 1891, 2345, 2505, 2817, 2840
AcoI	YGGCCR	1 cut(s)	1112
AcsI	RAATTY	3 cut(s)	968, 1579, 1784
AcuI	CTGAAG	3 cut(s)	350, 693, 722
AcyI	GRCGYC	1 cut(s)	48
AdeI	CACNNNGTG	1 cut(s)	1469
AfiI	CCNNNNNNNGG	3 cut(s)	529, 1328, 2936
AflII	CTTAAG	1 cut(s)	952
AflIII	ACRYGT	1 cut(s)	2204
AhlI	ACTAGT	1 cut(s)	433
AjnI	CCWGG	3 cut(s)	774, 1397, 2562
AjuI	GAANNNNNNNTTGG	4 cut(s)	321, 353, 2523, 2555
AleI	CACNNNNGTG	1 cut(s)	3003
AloI	GAACNNNNNNTCC	2 cut(s)	2026, 2058
Alw21I	GWGCWC	2 cut(s)	192, 1834
Alw26I	GTCTC	6 cut(s)	407, 1958, 2107, 2287, 2658, 2945
AlwI	GGATC	9 cut(s)	248, 263, 714, 1379, 1891, 2345, 2505, 2817, 2840
AlwNI	CAGNNNCTG	1 cut(s)	1991
Ama87I	CYCGRG	3 cut(s)	185, 257, 852
Aor13HI	TCCGGA	1 cut(s)	893
AoxI	GGCC	8 cut(s)	378, 742, 856, 1112, 1319, 1642, 2457, 2713
ApoI	RAATTY	3 cut(s)	968, 1579, 1784
Asp700I	GAANNNNTTC	1 cut(s)	333
AspLEI	GCGC	1 cut(s)	153
AspS9I	GGNCC	8 cut(s)	743, 772, 1205, 1319, 1643, 2122, 2212, 2713
AsuC2I	CCSGG	2 cut(s)	1616, 2194
AsuHPI	GGTGA	4 cut(s)	460, 1394, 1613, 1778
AsuII	TTCGAA	2 cut(s)	511, 966
AvaI	CYCGRG	3 cut(s)	185, 257, 852
AvaII	GGWCC	4 cut(s)	772, 1205, 2122, 2212
BaeGI	GKGCMC	1 cut(s)	2563
BanI	GGYRCC	2 cut(s)	60, 2558
BanII	GRGCYC	1 cut(s)	192
BauI	CACGAG	1 cut(s)	1719
BbsI	GAAGAC	1 cut(s)	1210
Bbv12I	GWGCWC	2 cut(s)	192, 1834
BccI	CCATC	5 cut(s)	1543, 1742, 2366, 2393, 2807
BceAI	ACGGC	2 cut(s)	843, 1127
BciT130I	CCWGG	3 cut(s)	776, 1399, 2564
BclI	TGATCA	1 cut(s)	988
BcnI	CCSGG	2 cut(s)	1616, 2194
BcoDI	GTCTC	6 cut(s)	407, 1958, 2107, 2287, 2658, 2945
BcuI	ACTAGT	1 cut(s)	433
BfaI	CTAG	4 cut(s)	398, 434, 2031, 2270
BfmI	CTRYAG	2 cut(s)	906, 1280
BfrI	CTTAAG	1 cut(s)	952
BfuAI	ACCTGC	2 cut(s)	1468, 2938
BglII	AGATCT	3 cut(s)	1713, 2038, 2766
BlpI	GCTNAGC	3 cut(s)	128, 191, 216
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	5 cut(s)	776, 1399, 1616, 2194, 2564
Bme18I	GGWCC	4 cut(s)	772, 1205, 2122, 2212
BmeT110I	CYCGRG	3 cut(s)	185, 257, 852
BmgT120I	GGNCC	8 cut(s)	743, 772, 1205, 1319, 1643, 2122, 2212, 2713
BmiI	GGNNCC	5 cut(s)	62, 1320, 2560, 2757, 3037
BmrFI	CCNGG	5 cut(s)	776, 1399, 1616, 2194, 2564
BmrI	ACTGGG	3 cut(s)	644, 1461, 1812
BmsI	GCATC	8 cut(s)	225, 718, 793, 1600, 2352, 2714, 2782, 2875
BmuI	ACTGGG	3 cut(s)	644, 1461, 1812
BpiI	GAAGAC	1 cut(s)	1210
BplI	GAGNNNNNCTC	2 cut(s)	3020, 3052
BpmI	CTGGAG	1 cut(s)	2474
Bpu10I	CCTNAGC	1 cut(s)	1314
Bpu1102I	GCTNAGC	3 cut(s)	128, 191, 216
Bpu14I	TTCGAA	2 cut(s)	511, 966
BpuEI	CTTGAG	1 cut(s)	1724
BpuMI	CCSGG	2 cut(s)	1616, 2194
BsaAI	YACGTR	2 cut(s)	1489, 2205
BsaBI	GATNNNNATC	3 cut(s)	1752, 1882, 2781
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	1 cut(s)	2945
BsaJI	CCNNGG	8 cut(s)	483, 775, 859, 1614, 1778, 2118, 2193, 2562
BsaWI	WCCGGW	1 cut(s)	893
BsaXI	ACNNNNNCTCC	2 cut(s)	2189, 2219
Bsc4I	CCNNNNNNNGG	3 cut(s)	529, 1328, 2936
Bse118I	RCCGGY	1 cut(s)	57
Bse3DI	GCAATG	4 cut(s)	49, 139, 1737, 2006
Bse8I	GATNNNNATC	3 cut(s)	1752, 1882, 2781
BseAI	TCCGGA	1 cut(s)	893
BseBI	CCWGG	3 cut(s)	776, 1399, 2564
BseDI	CCNNGG	8 cut(s)	483, 775, 859, 1614, 1778, 2118, 2193, 2562
BseGI	GGATG	7 cut(s)	499, 604, 1551, 1855, 2377, 2818, 2823
BseJI	GATNNNNATC	3 cut(s)	1752, 1882, 2781
BseLI	CCNNNNNNNGG	3 cut(s)	529, 1328, 2936
BseMI	GCAATG	4 cut(s)	49, 139, 1737, 2006
BseMII	CTCAG	6 cut(s)	205, 713, 936, 1986, 1990, 2700
BseSI	GKGCMC	1 cut(s)	2563
BseYI	CCCAGC	1 cut(s)	938
BsgI	GTGCAG	3 cut(s)	858, 918, 1446
Bsh1236I	CGCG	2 cut(s)	151, 2870
BshFI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2459, 2715
BshNI	GGYRCC	2 cut(s)	60, 2558
BsiHKAI	GWGCWC	2 cut(s)	192, 1834
BsiHKCI	CYCGRG	3 cut(s)	185, 257, 852
BsiSI	CCGG	4 cut(s)	58, 894, 1616, 2193
BslFI	GGGAC	2 cut(s)	1252, 2891
BslI	CCNNNNNNNGG	3 cut(s)	529, 1328, 2936
BsmAI	GTCTC	6 cut(s)	407, 1958, 2107, 2287, 2658, 2945
BsmFI	GGGAC	2 cut(s)	1252, 2891
BsmI	GAATGC	1 cut(s)	2905
BsnI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2459, 2715
Bso31I	GGTCTC	1 cut(s)	2945
BsoBI	CYCGRG	3 cut(s)	185, 257, 852
Bsp119I	TTCGAA	2 cut(s)	511, 966
Bsp1286I	GDGCHC	3 cut(s)	192, 1834, 2563
Bsp13I	TCCGGA	1 cut(s)	893
Bsp1407I	TGTACA	3 cut(s)	315, 1024, 1063
Bsp1720I	GCTNAGC	3 cut(s)	128, 191, 216
Bsp19I	CCATGG	2 cut(s)	483, 1778
BspACI	CCGC	3 cut(s)	64, 1543, 2916
BspANI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2459, 2715
BspCNI	CTCAG	6 cut(s)	204, 714, 937, 1987, 1989, 2701
BspEI	TCCGGA	1 cut(s)	893
BspFNI	CGCG	2 cut(s)	151, 2870
BspHI	TCATGA	1 cut(s)	499
BspLI	GGNNCC	5 cut(s)	62, 1320, 2560, 2757, 3037
BspMAI	CTGCAG	1 cut(s)	910
BspMI	ACCTGC	2 cut(s)	1468, 2938
BspPI	GGATC	9 cut(s)	248, 263, 714, 1379, 1891, 2345, 2505, 2817, 2840
BspQI	GCTCTTC	1 cut(s)	1287
BspT104I	TTCGAA	2 cut(s)	511, 966
BspT107I	GGYRCC	2 cut(s)	60, 2558
BspTI	CTTAAG	1 cut(s)	952
BspTNI	GGTCTC	1 cut(s)	2945
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	4 cut(s)	49, 139, 1737, 2006
BsrFI	RCCGGY	1 cut(s)	57
BsrGI	TGTACA	3 cut(s)	315, 1024, 1063
BssAI	RCCGGY	1 cut(s)	57
BssECI	CCNNGG	8 cut(s)	483, 775, 859, 1614, 1778, 2118, 2193, 2562
BssNAI	GTATAC	1 cut(s)	362
BssNI	GRCGYC	1 cut(s)	48
BssSI	CACGAG	1 cut(s)	1719
BssT1I	CCWWGG	3 cut(s)	483, 1778, 2118
Bst1107I	GTATAC	1 cut(s)	362
Bst2BI	CACGAG	1 cut(s)	1719
Bst2UI	CCWGG	3 cut(s)	776, 1399, 2564
Bst6I	CTCTTC	1 cut(s)	1287
BstACI	GRCGYC	1 cut(s)	48
BstAFI	CTTAAG	1 cut(s)	952
BstAUI	TGTACA	3 cut(s)	315, 1024, 1063
BstBAI	YACGTR	2 cut(s)	1489, 2205
BstBI	TTCGAA	2 cut(s)	511, 966
BstC8I	GCNNGC	2 cut(s)	846, 2334
BstDSI	CCRYGG	3 cut(s)	483, 859, 1778
BstEII	GGTNACC	2 cut(s)	1455, 1766
BstF5I	GGATG	7 cut(s)	499, 604, 1551, 1855, 2377, 2818, 2823
BstFNI	CGCG	2 cut(s)	151, 2870
BstHHI	GCGC	1 cut(s)	153
BstMAI	GTCTC	6 cut(s)	407, 1958, 2107, 2287, 2658, 2945
BstMWI	GCNNNNNNNGC	5 cut(s)	244, 845, 905, 1120, 1129
BstNI	CCWGG	3 cut(s)	776, 1399, 2564
BstPI	GGTNACC	2 cut(s)	1455, 1766
BstSCI	CCNGG	5 cut(s)	774, 1397, 1614, 2192, 2562
BstSFI	CTRYAG	2 cut(s)	906, 1280
BstSLI	GKGCMC	1 cut(s)	2563
BstSNI	TACGTA	1 cut(s)	1489
BstUI	CGCG	2 cut(s)	151, 2870
BstV2I	GAAGAC	1 cut(s)	1210
BstX2I	RGATCY	8 cut(s)	706, 1713, 2038, 2350, 2497, 2766, 2822, 2845
BstXI	CCANNNNNNTGG	2 cut(s)	490, 2096
BstYI	RGATCY	8 cut(s)	706, 1713, 2038, 2350, 2497, 2766, 2822, 2845
BstZ17I	GTATAC	1 cut(s)	362
BsuRI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2459, 2715
BtgI	CCRYGG	3 cut(s)	483, 859, 1778
BtsCI	GGATG	7 cut(s)	499, 604, 1551, 1855, 2377, 2818, 2823
BtsI	GCAGTG	1 cut(s)	1899
BtsIMutI	CAGTG	6 cut(s)	423, 663, 1474, 1899, 2254, 2550
BveI	ACCTGC	2 cut(s)	1468, 2938
Cac8I	GCNNGC	2 cut(s)	846, 2334
CaiI	CAGNNNCTG	1 cut(s)	1991
CciI	TCATGA	1 cut(s)	499
CfoI	GCGC	1 cut(s)	153
Cfr10I	RCCGGY	1 cut(s)	57
Cfr13I	GGNCC	8 cut(s)	743, 772, 1205, 1319, 1643, 2122, 2212, 2713
CseI	GACGC	2 cut(s)	37, 561
DraIII	CACNNNGTG	1 cut(s)	1469
EaeI	YGGCCR	1 cut(s)	1112
Eam1104I	CTCTTC	1 cut(s)	1287
EarI	CTCTTC	1 cut(s)	1287
Ecl136II	GAGCTC	1 cut(s)	190
Eco105I	TACGTA	1 cut(s)	1489
Eco130I	CCWWGG	3 cut(s)	483, 1778, 2118
Eco147I	AGGCCT	2 cut(s)	380, 2459
Eco24I	GRGCYC	1 cut(s)	192
Eco31I	GGTCTC	1 cut(s)	2945
Eco32I	GATATC	3 cut(s)	681, 1426, 2605
Eco47I	GGWCC	4 cut(s)	772, 1205, 2122, 2212
Eco53kI	GAGCTC	1 cut(s)	190
Eco57I	CTGAAG	3 cut(s)	350, 693, 722
Eco88I	CYCGRG	3 cut(s)	185, 257, 852
Eco91I	GGTNACC	2 cut(s)	1455, 1766
EcoICRI	GAGCTC	1 cut(s)	190
EcoO109I	RGGNCCY	2 cut(s)	1319, 2713
EcoO65I	GGTNACC	2 cut(s)	1455, 1766
EcoRII	CCWGG	3 cut(s)	774, 1397, 2562
EcoRV	GATATC	3 cut(s)	681, 1426, 2605
EcoT14I	CCWWGG	3 cut(s)	483, 1778, 2118
EcoT22I	ATGCAT	1 cut(s)	2905
EcoT38I	GRGCYC	1 cut(s)	192
ErhI	CCWWGG	3 cut(s)	483, 1778, 2118
FalI	AAGNNNNNCTT	2 cut(s)	2523, 2555
FaqI	GGGAC	2 cut(s)	1252, 2891
FauNDI	CATATG	2 cut(s)	633, 2487
FbaI	TGATCA	1 cut(s)	988
FblI	GTMKAC	2 cut(s)	361, 2390
FokI	GGATG	7 cut(s)	506, 611, 1558, 1842, 2384, 2825, 2830
FriOI	GRGCYC	1 cut(s)	192
FspBI	CTAG	4 cut(s)	398, 434, 2031, 2270
GlaI	GCGC	1 cut(s)	152
GsaI	CCCAGC	1 cut(s)	942
GsuI	CTGGAG	1 cut(s)	2474
HaeIII	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2459, 2715
HapII	CCGG	4 cut(s)	58, 894, 1616, 2193
HgaI	GACGC	2 cut(s)	37, 561
HhaI	GCGC	1 cut(s)	153
Hin1I	GRCGYC	1 cut(s)	48
Hin6I	GCGC	1 cut(s)	151
HinP1I	GCGC	1 cut(s)	151
HincII	GTYRAC	1 cut(s)	2724
HindII	GTYRAC	1 cut(s)	2724
HindIII	AAGCTT	1 cut(s)	1130
HpaII	CCGG	4 cut(s)	58, 894, 1616, 2193
HphI	GGTGA	4 cut(s)	460, 1394, 1613, 1778
Hpy166II	GTNNAC	7 cut(s)	16, 362, 431, 1412, 1492, 2391, 2724
Hpy8I	GTNNAC	7 cut(s)	16, 362, 431, 1412, 1492, 2391, 2724
Hpy99I	CGWCG	1 cut(s)	86
HpyAV	CCTTC	8 cut(s)	4, 256, 668, 697, 1241, 1770, 1913, 1993
HpyCH4IV	ACGT	6 cut(s)	211, 541, 767, 1488, 1950, 2204
HpyF10VI	GCNNNNNNNGC	5 cut(s)	244, 845, 905, 1120, 1129
HpySE526I	ACGT	6 cut(s)	211, 541, 767, 1488, 1950, 2204
Hsp92I	GRCGYC	1 cut(s)	48
HspAI	GCGC	1 cut(s)	151
Kpn2I	TCCGGA	1 cut(s)	893
Ksp22I	TGATCA	1 cut(s)	988
LguI	GCTCTTC	1 cut(s)	1287
LmnI	GCTCC	7 cut(s)	71, 896, 1936, 2091, 2161, 2197, 3041
LweI	GCATC	8 cut(s)	225, 718, 793, 1600, 2352, 2714, 2782, 2875
MaeI	CTAG	4 cut(s)	398, 434, 2031, 2270
MaeII	ACGT	6 cut(s)	211, 541, 767, 1488, 1950, 2204
MbiI	CCGCTC	1 cut(s)	66
MfeI	CAATTG	1 cut(s)	1286
MflI	RGATCY	8 cut(s)	706, 1713, 2038, 2350, 2497, 2766, 2822, 2845
MhlI	GDGCHC	3 cut(s)	192, 1834, 2563
MlyI	GAGTC	7 cut(s)	379, 662, 695, 1664, 1960, 1989, 3004
MmeI	TCCRAC	4 cut(s)	750, 2531, 2850, 3063
Mph1103I	ATGCAT	1 cut(s)	2905
MroI	TCCGGA	1 cut(s)	893
MroXI	GAANNNNTTC	1 cut(s)	333
MseI	TTAA	6 cut(s)	546, 749, 953, 1236, 1449, 1742
MslI	CAYNNNNRTG	6 cut(s)	488, 1122, 1860, 2064, 3003, 3024
MspA1I	CMGCKG	3 cut(s)	721, 1169, 1543
MspCI	CTTAAG	1 cut(s)	952
MspI	CCGG	4 cut(s)	58, 894, 1616, 2193
MspR9I	CCNGG	5 cut(s)	776, 1399, 1616, 2194, 2564
MunI	CAATTG	1 cut(s)	1286
Mva1269I	GAATGC	1 cut(s)	2905
MvaI	CCWGG	3 cut(s)	776, 1399, 2564
MvnI	CGCG	2 cut(s)	151, 2870
MwoI	GCNNNNNNNGC	5 cut(s)	244, 845, 905, 1120, 1129
NciI	CCSGG	2 cut(s)	1616, 2194
NcoI	CCATGG	2 cut(s)	483, 1778
NdeI	CATATG	2 cut(s)	633, 2487
NlaIV	GGNNCC	5 cut(s)	62, 1320, 2560, 2757, 3037
NsiI	ATGCAT	1 cut(s)	2905
NspV	TTCGAA	2 cut(s)	511, 966
OliI	CACNNNNGTG	1 cut(s)	3003
PaeR7I	CTCGAG	2 cut(s)	185, 852
PagI	TCATGA	1 cut(s)	499
PceI	AGGCCT	2 cut(s)	380, 2459
PciSI	GCTCTTC	1 cut(s)	1287
PcsI	WCGNNNNNNNCGW	2 cut(s)	1840, 2071
PctI	GAATGC	1 cut(s)	2905
PdmI	GAANNNNTTC	1 cut(s)	333
PfeI	GAWTC	9 cut(s)	24, 112, 323, 518, 1361, 1394, 1724, 2403, 2596
PleI	GAGTC	7 cut(s)	379, 662, 694, 1663, 1960, 1988, 3003
PpsI	GAGTC	7 cut(s)	379, 662, 694, 1663, 1960, 1988, 3003
Ppu21I	YACGTR	2 cut(s)	1489, 2205
PsiI	TTATAA	1 cut(s)	2663
Psp124BI	GAGCTC	1 cut(s)	192
Psp6I	CCWGG	3 cut(s)	774, 1397, 2562
PspEI	GGTNACC	2 cut(s)	1455, 1766
PspFI	CCCAGC	1 cut(s)	938
PspGI	CCWGG	3 cut(s)	774, 1397, 2562
PspN4I	GGNNCC	5 cut(s)	62, 1320, 2560, 2757, 3037
PspPI	GGNCC	8 cut(s)	743, 772, 1205, 1319, 1643, 2122, 2212, 2713
PspXI	VCTCGAGB	2 cut(s)	185, 852
PstI	CTGCAG	1 cut(s)	910
PstNI	CAGNNNCTG	1 cut(s)	1991
PsuI	RGATCY	8 cut(s)	706, 1713, 2038, 2350, 2497, 2766, 2822, 2845
PvuII	CAGCTG	2 cut(s)	721, 1169
RseI	CAYNNNNRTG	6 cut(s)	488, 1122, 1860, 2064, 3003, 3024
SacI	GAGCTC	1 cut(s)	192
SapI	GCTCTTC	1 cut(s)	1287
SaqAI	TTAA	6 cut(s)	546, 749, 953, 1236, 1449, 1742
Sau96I	GGNCC	8 cut(s)	743, 772, 1205, 1319, 1643, 2122, 2212, 2713
ScaI	AGTACT	1 cut(s)	832
SchI	GAGTC	7 cut(s)	379, 662, 695, 1664, 1960, 1989, 3004
ScrFI	CCNGG	5 cut(s)	776, 1399, 1616, 2194, 2564
SduI	GDGCHC	3 cut(s)	192, 1834, 2563
SfaNI	GCATC	8 cut(s)	225, 718, 793, 1600, 2352, 2714, 2782, 2875
SfcI	CTRYAG	2 cut(s)	906, 1280
Sfr274I	CTCGAG	2 cut(s)	185, 852
SfuI	TTCGAA	2 cut(s)	511, 966
SinI	GGWCC	4 cut(s)	772, 1205, 2122, 2212
SlaI	CTCGAG	2 cut(s)	185, 852
SmiMI	CAYNNNNRTG	6 cut(s)	488, 1122, 1860, 2064, 3003, 3024
SmlI	CTYRAG	4 cut(s)	185, 852, 952, 1703
SmoI	CTYRAG	4 cut(s)	185, 852, 952, 1703
SnaBI	TACGTA	1 cut(s)	1489
SpeI	ACTAGT	1 cut(s)	433
SseBI	AGGCCT	2 cut(s)	380, 2459
SsiI	CCGC	3 cut(s)	64, 1543, 2916
SspI	AATATT	2 cut(s)	613, 2649
SspMI	CTAG	4 cut(s)	398, 434, 2031, 2270
SstI	GAGCTC	1 cut(s)	192
StuI	AGGCCT	2 cut(s)	380, 2459
StyD4I	CCNGG	5 cut(s)	774, 1397, 1614, 2192, 2562
StyI	CCWWGG	3 cut(s)	483, 1778, 2118
TaiI	ACGT	6 cut(s)	214, 544, 770, 1491, 1953, 2207
TaqI	TCGA	4 cut(s)	186, 511, 853, 966
TaqII	GACCGA	1 cut(s)	1248
TatI	WGTACW	5 cut(s)	315, 830, 1024, 1063, 1482
TfiI	GAWTC	9 cut(s)	24, 112, 323, 518, 1361, 1394, 1724, 2403, 2596
Tru1I	TTAA	6 cut(s)	546, 749, 953, 1236, 1449, 1742
Tru9I	TTAA	6 cut(s)	546, 749, 953, 1236, 1449, 1742
TscAI	CASTG	6 cut(s)	423, 663, 1474, 1899, 2254, 2550
TspRI	CASTG	6 cut(s)	423, 663, 1474, 1899, 2254, 2550
Vha464I	CTTAAG	1 cut(s)	952
VpaK11BI	GGWCC	4 cut(s)	772, 1205, 2122, 2212
XapI	RAATTY	3 cut(s)	968, 1579, 1784
XhoI	CTCGAG	2 cut(s)	185, 852
XmiI	GTMKAC	2 cut(s)	361, 2390
XmnI	GAANNNNTTC	1 cut(s)	333
XspI	CTAG	4 cut(s)	398, 434, 2031, 2270
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	2905

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.