Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005040

Physical Location & Seq

Genomic Coordinates Reverse (-)

30589011 .. 30599917

Gene Structure 10907 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG274500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGAGGAAGAAGGTCGACGAGCGCATCAGAACCCTGATAAGCAATGGCGTCAGAAACAAGCACCGCTCCATGTTCGTCATCGTCGGCGACAAGTCACGTGACCAGATTGTGAATCTTCACTATATGCTCAGCAATGAATTGAAGAAATCAAAGCCTACTGTGTTGTGGTGCTACAAGGACAAGCTTGAGCTCAGCAGTCACAAGAAAAAACGTGCTAAGCAGGTGAAAAAGCTAATGCAGAGGGGCCTGCTTGATCCCGAGAAGGTTGATCCCTTCTCACTTTTTATCGAAAGTAGCGGAGTGACCTATTGCTTGTACAAGGATTCTGAGAGAATTCTTGGTAATACATTTGGGATGTGTATACTTCAGGATTTTGAGGCCGTGACACCAAATCTACTAGCAAGAACAATAGAGACTGTGGAGGGTGGCGGACTAATTATATTGTTGCTTCGTTCTCTAACCTCACTCACCAACTTATACACCATGGTTATGGATGTCCATGACAGGTTTCGAACCGAATCACATTCCCAGGCTACTGGCCGTTTCAATGAGCGTTTCTTATTATCACTTGCATCATGCAAAGCATGTGTGGTTATGGATGATGAACTGAATATTTTACCAATTTCATCTCACATAAGGTCAATTACTCCAGTTCCAGTTAAAGAGGACTCTGAGGGAATATCCGAGTCAGAAAGGGAATTGAAGGATCTAAAAGAACAACTAAGTGACGCCTTTCCCGTCGGCCCTTTAATAAAGAAATGTTGTACGCTCGACCAGGGAAATGCTGTTGCTACATTTCTCGATGCAATATTGGATAAGGCACTCCGAAGTACTGTTGCCTTGCTCGCTGCTCGTGGCCGTGGGAAGTCAGCTGCACTTGGTTTGGCTATTGCTGGAGCTATTGCTGCAGGGTATTCAAATATCTTTGTAACTGCACCGAGCCCTGAGAACTTGAAGACCTTGTTCGAATTTGTTTGCAAGGGTTTGGATGCATTGGATTATAAGGAACATTTAGATTATGATGTGGAGAAAAGTTCCAACCCTGCGTTGAAGAAAGCAACTCTAAAGATCAATATATTCAAGCAGCACAGACAAACAATTCAGTACATACAGCCCCATGAGCATGAAAAACTTTCCCAAGTTGAATTATTGGTTGTTGATGAGGCTGCTGCTATTCCACTGCCAGTGGTGAAGTCTTTGCTTGGCCCTTATCTGGTCTTCCTTTCATCTACAGTTAATGGCTATGAAGGTACTGGTCGGTCTTTGTCTCTAAAGCTTCTACAGCAATTGGAAGAGCAAAGCCTGATGTCTGCTAATGGCCCTATATCTGGTCGACTTTTCAAAAAGATAGAATTGAAAGAATCAATCAGATATGCTTCTGGTGACCCCGTTGAATCCTGGCTTGGCGGCTTACTTTGTTTGGATATTACCAATTCCATTCCTAAGCTTAACGGGTTACCTGCACCTAATGAGTGTGATCTCTACTATGTGAACCGTGATACACTTTTTTCATATCACAAAGATAGCGAGTTGTTTTTACAGAGAATGATGGCGCTATATGTTGCCTCTCACTACAAGAATTCTCCAAATGATTTGCAACTAATGGCTGATGCTCCAGCGCATCACTTGTTCGTATTGCTTGGTCCTGTTGACGAGTCAAAAAATCAACTTCCTGATATTTTGTGTGTCATTCAGGTGAGCCTGGAGGGACAAATTTCTCGTAAATCTGCAATCAAAAGTTTAAGTGATGGTCATCAGCCTTTTGGTGATCAGATACCATGGAAGTTCTGTGAGCAATTTCAGGACACAGTTTTCCCCACACTTTCAGGTGCTCGAATTGTTCGAATTGCTACCCATCCAAGTGCCATGAAGATTGGTTATGGTTCACAAGCAGTAGAACTATTGACAAGGTACTATGAAGGACAATTTGCTCCTATTTCGGAAATAGATGTCGAAGAAGTTGAGGCGCCAGCTGTCAGTGTGGTGGAAGCTGCCAAGAAGGTTTCATTGCTTGAGGAAAATATAAAACCCAGAACAGATCTTCCCCATTTGCTGGTGCATCTTCGTGAAAGACGACCGGAGAAGCTCCATTATATTGGTGTCTCCTTCGGCCTTACCTTGGACCTTTTCCGTTTTTGGAGTAAACATAAATTTGCTCCATTCTATATTGGGCATATTCCAAGTGCTGTGACGGGTGAGCATACATGTATGGTCCTGAAATCGTTGAACAGTGATGACCTTGAAGTTAGTGATTTTCATGCATTTTACCTAGATTTCAGGCGAAGGTTTCTCAGATTATTAGGTGTTAGTTTCCAGAAGATGGATTATAGGCTCGCCATGAGCATCTTAGATCCCAAGATCAATTTCAAGGAGCTAGAGTCCACATCAAAAATCTCAAATGGATTCTTAAAGTCGACTAAAGATTTCTTCTCGCCTTATGATATGAAGCGACTGGAAGCTTATACAAACCATCTTGCTGACTTTCATATGATTTTGGATCTTGTTCGAACCATTTCAGATCTCTATTTTCAAGAAAAACTTCCGGTAACATTGTCATATGCTCAAGCTTCTATTTTGCTGTGCATTGGCCTGCAAAGTCGAGATATCTCCTATATCGAGGGGGTCATGAAATTAGAAAGGCAGCAGATATTGTCACTGTTTATAAAAGTTATCAAGAAGATCTACAAATATTTGTACTCTATTGCGTCTGACGAGATTCAGTCAACCTTGCCTCAACCAAAAGAAACTCTAATGGAACCTCACAAAATCTCTGTCGATGAGGATCTTGAAAATGCTGCGAGAGAAGTTGAGGATCAAAGGAGATCCAAATCGTTAAATCAACAAGATGCAATTGAGGACGAAGATTCGTTAAATCCCGAGTTATTTCAACAATATGCAATTAAGGACGAAGATGCAGAGTTTGCAAATGCTCTGCAGAATGGTAGACAGATACCAAGGGATGGTGTTATTAGTGTGAAATCAAGTAGAAAAGAAAAAGAGGAGCATGGAAAACATAGAGAGAGTGACAAGGGTGGAAACAGACGTGGTAGAGATGAGCATGGCTCCACCTCTAAGTCCAACAAGAAGAGTAAATCTTCACGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0000154 GO:0002097 GO:0002101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008080 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051391 GO:0051392 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1904812 GO:1990882 GO:1990883 GO:1990884 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DUF1726 GNAT_acetyltr_2 Helicase_RecD tRNA_bind_2

Protein Analysis

1034

Amino Acids

116.81

Weight (kDa)

7.36

Isoelectric Point (pI)

45.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TmcA_N	PF08351	1 - 200	2.7e-19	tRNA(Met) cytidine acetyltransferase TmcA, N-terminal
NAT10_TcmA_helicase	PF05127	281 - 475	2.2e-56	RNA cytidine acetyltransferase NAT10/TcmA, helicase domain
GNAT_acetyltr_2	PF13718	515 - 743	4.9e-95	GNAT acetyltransferase 2
tRNA_bind_2	PF13725	753 - 977	1.4e-63	Possible tRNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005040)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10490 AT1G10490 AT3G57940 AT3G57940
fragaria_vesca	FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910
malus_domestica	MD05G1028000.v1.1 MD10G1028600.v1.1
prunus_persica	Prupe.6G169200_v2.0.a1
pyrus_communis	pycom05g02010 pycom10g01860
rosa_chinensis	RchiOBHm_Chr2g0117351
rosa_laevigata	RLG00000018344
rosa_multiflora	Rmu_co7970672.1_g000001 Rmu_sc0004436.1_g000001 Rmu_sc0013504.1_g000001
rosa_roxburghii	Rroxscaffold_2G00126550
rosa_rugosa	Rorug02G0212300 Rorug02G0212400
rosa_samantha	Rh2AG267400 Rh2BG279800 Rh2DG274500 Rh2DG293800
rosa_wichuraiana	Rw2G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1002, 2663
AarI	CACCTGC	1 cut(s)	211
Acc36I	ACCTGC	2 cut(s)	211, 1468
AccB1I	GGYRCC	1 cut(s)	1966
AccB7I	CCANNNNNTGG	3 cut(s)	2053, 2320, 2960
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	5 cut(s)	15, 361, 1333, 2414, 2944
AciI	CCGC	4 cut(s)	64, 297, 429, 1407
AclWI	GGATC	8 cut(s)	248, 263, 714, 2345, 2505, 2790, 2817, 2820
AcoI	YGGCCR	2 cut(s)	538, 856
AcsI	RAATTY	5 cut(s)	333, 968, 1579, 1713, 2150
AcuI	CTGAAG	1 cut(s)	350
AcvI	CACGTG	1 cut(s)	98
AcyI	GRCGYC	3 cut(s)	48, 729, 1967
AfaI	GTAC	7 cut(s)	317, 766, 832, 1106, 1252, 1913, 2696
AfiI	CCNNNNNNNGG	6 cut(s)	1213, 1328, 2053, 2119, 2320, 2960
AflIII	ACRYGT	1 cut(s)	2204
AjiI	CACGTC	1 cut(s)	3044
AjnI	CCWGG	4 cut(s)	528, 774, 1397, 1701
AjuI	GAANNNNNNNTTGG	4 cut(s)	1386, 1418, 2348, 2380
AloI	GAACNNNNNNTCC	2 cut(s)	2026, 2058
Alw21I	GWGCWC	2 cut(s)	192, 1834
Alw26I	GTCTC	3 cut(s)	407, 1272, 2107
AlwI	GGATC	8 cut(s)	248, 263, 714, 2345, 2505, 2790, 2817, 2820
Ama87I	CYCGRG	2 cut(s)	257, 2876
AoxI	GGCC	9 cut(s)	244, 378, 538, 742, 856, 1204, 1318, 2110, 2587
ApeKI	GCWGC	9 cut(s)	848, 872, 905, 1084, 1166, 1169, 1991, 2641, 2795
ApoI	RAATTY	5 cut(s)	333, 968, 1579, 1713, 2150
ArsI	GACNNNNNNTTYG	2 cut(s)	2757, 2789
Asp700I	GAANNNNTTC	2 cut(s)	2512, 2538
AspLEI	GCGC	4 cut(s)	24, 1555, 1621, 1969
AspS9I	GGNCC	7 cut(s)	244, 743, 1205, 1319, 1643, 2122, 2212
AsuHPI	GGTGA	7 cut(s)	235, 460, 1201, 1394, 1708, 1778, 2207
AsuII	TTCGAA	4 cut(s)	511, 966, 1843, 2506
AvaI	CYCGRG	2 cut(s)	257, 2876
AvaII	GGWCC	3 cut(s)	1643, 2122, 2212
BanI	GGYRCC	1 cut(s)	1966
BanII	GRGCYC	2 cut(s)	192, 944
BauI	CACGAG	1 cut(s)	852
BbrPI	CACGTG	1 cut(s)	98
BbsI	GAAGAC	2 cut(s)	962, 1210
Bbv12I	GWGCWC	2 cut(s)	192, 1834
BbvI	GCAGC	9 cut(s)	835, 859, 892, 1096, 1153, 1156, 1978, 2653, 2782
BccI	CCATC	6 cut(s)	1543, 1742, 1863, 2314, 2478, 2954
BceAI	ACGGC	3 cut(s)	365, 525, 843
BciT130I	CCWGG	4 cut(s)	530, 776, 1399, 1703
BclI	TGATCA	1 cut(s)	1768
BcoDI	GTCTC	3 cut(s)	407, 1272, 2107
BfaI	CTAG	4 cut(s)	398, 2270, 2375, 3103
BfmI	CTRYAG	4 cut(s)	906, 1230, 1280, 2933
BfoI	RGCGCY	2 cut(s)	1556, 1970
BfuAI	ACCTGC	2 cut(s)	211, 1468
BglII	AGATCT	3 cut(s)	2038, 2518, 2679
BlpI	GCTNAGC	3 cut(s)	128, 191, 216
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	4 cut(s)	530, 776, 1399, 1703
Bme18I	GGWCC	3 cut(s)	1643, 2122, 2212
BmeT110I	CYCGRG	2 cut(s)	257, 2876
BmgBI	CACGTC	1 cut(s)	3044
BmgT120I	GGNCC	7 cut(s)	244, 743, 1205, 1319, 1643, 2122, 2212
BmiI	GGNNCC	4 cut(s)	245, 1968, 2757, 3064
BmrFI	CCNGG	4 cut(s)	530, 776, 1399, 1703
BpiI	GAAGAC	2 cut(s)	962, 1210
BplI	GAGNNNNNCTC	2 cut(s)	3047, 3079
BpmI	CTGGAG	4 cut(s)	633, 915, 1599, 1724
Bpu10I	CCTNAGC	1 cut(s)	1443
Bpu1102I	GCTNAGC	3 cut(s)	128, 191, 216
Bpu14I	TTCGAA	4 cut(s)	511, 966, 1843, 2506
BpuEI	CTTGAG	3 cut(s)	206, 2033, 2547
BsaAI	YACGTR	1 cut(s)	98
BsaBI	GATNNNNATC	3 cut(s)	1752, 2781, 2827
BsaHI	GRCGYC	3 cut(s)	48, 729, 1967
BsaJI	CCNNGG	7 cut(s)	483, 528, 775, 859, 1778, 2118, 2954
BsaWI	WCCGGW	2 cut(s)	2077, 2542
BsaXI	ACNNNNNCTCC	2 cut(s)	2363, 2393
Bsc4I	CCNNNNNNNGG	6 cut(s)	1213, 1328, 2053, 2119, 2320, 2960
Bse1I	ACTGG	6 cut(s)	541, 650, 656, 1184, 1258, 2457
Bse3DI	GCAATG	3 cut(s)	49, 139, 2006
Bse8I	GATNNNNATC	3 cut(s)	1752, 2781, 2827
BseBI	CCWGG	4 cut(s)	530, 776, 1399, 1703
BseDI	CCNNGG	7 cut(s)	483, 528, 775, 859, 1778, 2118, 2954
BseGI	GGATG	6 cut(s)	360, 499, 604, 994, 1855, 2965
BseJI	GATNNNNATC	3 cut(s)	1752, 2781, 2827
BseLI	CCNNNNNNNGG	6 cut(s)	1213, 1328, 2053, 2119, 2320, 2960
BseMI	GCAATG	3 cut(s)	49, 139, 2006
BseMII	CTCAG	6 cut(s)	142, 205, 318, 663, 936, 2305
BseNI	ACTGG	6 cut(s)	541, 650, 656, 1184, 1258, 2457
BseRI	GAGGAG	1 cut(s)	3014
BseXI	GCAGC	9 cut(s)	835, 859, 892, 1096, 1153, 1156, 1978, 2653, 2782
BsgI	GTGCAG	3 cut(s)	858, 918, 1446
Bsh1285I	CGRYCG	1 cut(s)	2078
BshFI	GGCC	9 cut(s)	246, 380, 540, 744, 858, 1206, 1320, 2112, 2589
BshNI	GGYRCC	1 cut(s)	1966
BsiEI	CGRYCG	1 cut(s)	2078
BsiHKAI	GWGCWC	2 cut(s)	192, 1834
BsiHKCI	CYCGRG	2 cut(s)	257, 2876
BsiSI	CCGG	2 cut(s)	2078, 2543
BslFI	GGGAC	1 cut(s)	1722
BslI	CCNNNNNNNGG	6 cut(s)	1213, 1328, 2053, 2119, 2320, 2960
BsmAI	GTCTC	3 cut(s)	407, 1272, 2107
BsmFI	GGGAC	1 cut(s)	1722
BsnI	GGCC	9 cut(s)	246, 380, 540, 744, 858, 1206, 1320, 2112, 2589
BsoBI	CYCGRG	2 cut(s)	257, 2876
Bsp119I	TTCGAA	4 cut(s)	511, 966, 1843, 2506
Bsp1286I	GDGCHC	3 cut(s)	192, 944, 1834
Bsp1407I	TGTACA	1 cut(s)	315
Bsp1720I	GCTNAGC	3 cut(s)	128, 191, 216
Bsp19I	CCATGG	2 cut(s)	483, 1778
BspACI	CCGC	4 cut(s)	64, 297, 429, 1407
BspANI	GGCC	9 cut(s)	246, 380, 540, 744, 858, 1206, 1320, 2112, 2589
BspCNI	CTCAG	6 cut(s)	141, 204, 319, 664, 937, 2304
BspHI	TCATGA	1 cut(s)	2625
BspLI	GGNNCC	4 cut(s)	245, 1968, 2757, 3064
BspMAI	CTGCAG	2 cut(s)	910, 2937
BspMI	ACCTGC	2 cut(s)	211, 1468
BspPI	GGATC	8 cut(s)	248, 263, 714, 2345, 2505, 2790, 2817, 2820
BspQI	GCTCTTC	1 cut(s)	1287
BspT104I	TTCGAA	4 cut(s)	511, 966, 1843, 2506
BspT107I	GGYRCC	1 cut(s)	1966
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	3 cut(s)	49, 139, 2006
BsrGI	TGTACA	1 cut(s)	315
BsrI	ACTGG	6 cut(s)	541, 650, 656, 1184, 1258, 2457
BssECI	CCNNGG	7 cut(s)	483, 528, 775, 859, 1778, 2118, 2954
BssNAI	GTATAC	1 cut(s)	362
BssNI	GRCGYC	3 cut(s)	48, 729, 1967
BssSI	CACGAG	1 cut(s)	852
BssT1I	CCWWGG	4 cut(s)	483, 1778, 2118, 2954
Bst1107I	GTATAC	1 cut(s)	362
Bst2BI	CACGAG	1 cut(s)	852
Bst2UI	CCWGG	4 cut(s)	530, 776, 1399, 1703
Bst4CI	ACNGT	8 cut(s)	160, 418, 835, 1234, 1496, 1810, 2231, 2658
Bst6I	CTCTTC	2 cut(s)	1287, 3080
BstACI	GRCGYC	3 cut(s)	48, 729, 1967
BstAPI	GCANNNNNTGC	1 cut(s)	2921
BstAUI	TGTACA	1 cut(s)	315
BstBAI	YACGTR	1 cut(s)	98
BstBI	TTCGAA	4 cut(s)	511, 966, 1843, 2506
BstC8I	GCNNGC	5 cut(s)	248, 846, 1971, 2334, 2591
BstDSI	CCRYGG	3 cut(s)	483, 859, 1778
BstEII	GGTNACC	2 cut(s)	1382, 1455
BstF5I	GGATG	6 cut(s)	360, 499, 604, 994, 1855, 2965
BstH2I	RGCGCY	2 cut(s)	1556, 1970
BstHHI	GCGC	4 cut(s)	24, 1555, 1621, 1969
BstMAI	GTCTC	3 cut(s)	407, 1272, 2107
BstMCI	CGRYCG	1 cut(s)	2078
BstMWI	GCNNNNNNNGC	5 cut(s)	845, 905, 1120, 1282, 2921
BstNI	CCWGG	4 cut(s)	530, 776, 1399, 1703
BstNSI	RCATGY	2 cut(s)	588, 2208
BstPI	GGTNACC	2 cut(s)	1382, 1455
BstSCI	CCNGG	4 cut(s)	528, 774, 1397, 1701
BstSFI	CTRYAG	4 cut(s)	906, 1230, 1280, 2933
BstV1I	GCAGC	9 cut(s)	835, 859, 892, 1096, 1153, 1156, 1978, 2653, 2782
BstV2I	GAAGAC	2 cut(s)	962, 1210
BstX2I	RGATCY	8 cut(s)	706, 2038, 2350, 2497, 2518, 2679, 2782, 2822
BstXI	CCANNNNNNTGG	3 cut(s)	490, 536, 2096
BstYI	RGATCY	8 cut(s)	706, 2038, 2350, 2497, 2518, 2679, 2782, 2822
BstZ17I	GTATAC	1 cut(s)	362
BsuRI	GGCC	9 cut(s)	246, 380, 540, 744, 858, 1206, 1320, 2112, 2589
BtgI	CCRYGG	3 cut(s)	483, 859, 1778
BtrI	CACGTC	1 cut(s)	3044
BtsCI	GGATG	6 cut(s)	360, 499, 604, 994, 1855, 2965
BtsI	GCAGTG	1 cut(s)	1178
BtsIMutI	CAGTG	5 cut(s)	1178, 1191, 1984, 2236, 2654
BveI	ACCTGC	2 cut(s)	211, 1468
Cac8I	GCNNGC	5 cut(s)	248, 846, 1971, 2334, 2591
CciI	TCATGA	1 cut(s)	2625
CfoI	GCGC	4 cut(s)	24, 1555, 1621, 1969
Cfr13I	GGNCC	7 cut(s)	244, 743, 1205, 1319, 1643, 2122, 2212
CseI	GACGC	3 cut(s)	37, 737, 2694
Csp6I	GTAC	7 cut(s)	316, 765, 831, 1105, 1251, 1912, 2695
CviQI	GTAC	7 cut(s)	316, 765, 831, 1105, 1251, 1912, 2695
DinI	GGCGCC	1 cut(s)	1968
EaeI	YGGCCR	2 cut(s)	538, 856
Eam1104I	CTCTTC	2 cut(s)	1287, 3080
EarI	CTCTTC	2 cut(s)	1287, 3080
EciI	GGCGGA	1 cut(s)	444
Ecl136II	GAGCTC	1 cut(s)	190
Eco130I	CCWWGG	4 cut(s)	483, 1778, 2118, 2954
Eco24I	GRGCYC	2 cut(s)	192, 944
Eco32I	GATATC	1 cut(s)	2605
Eco47I	GGWCC	3 cut(s)	1643, 2122, 2212
Eco53kI	GAGCTC	1 cut(s)	190
Eco57I	CTGAAG	1 cut(s)	350
Eco72I	CACGTG	1 cut(s)	98
Eco88I	CYCGRG	2 cut(s)	257, 2876
Eco91I	GGTNACC	2 cut(s)	1382, 1455
EcoICRI	GAGCTC	1 cut(s)	190
EcoO109I	RGGNCCY	1 cut(s)	244
EcoO65I	GGTNACC	2 cut(s)	1382, 1455
EcoRI	GAATTC	2 cut(s)	333, 1579
EcoRII	CCWGG	4 cut(s)	528, 774, 1397, 1701
EcoRV	GATATC	1 cut(s)	2605
EcoT14I	CCWWGG	4 cut(s)	483, 1778, 2118, 2954
EcoT22I	ATGCAT	2 cut(s)	994, 2263
EcoT38I	GRGCYC	2 cut(s)	192, 944
EgeI	GGCGCC	1 cut(s)	1968
EheI	GGCGCC	1 cut(s)	1968
ErhI	CCWWGG	4 cut(s)	483, 1778, 2118, 2954
FalI	AAGNNNNNCTT	2 cut(s)	2523, 2555
FaqI	GGGAC	1 cut(s)	1722
FauNDI	CATATG	2 cut(s)	2487, 2557
FbaI	TGATCA	1 cut(s)	1768
FblI	GTMKAC	5 cut(s)	15, 361, 1333, 2414, 2944
FokI	GGATG	6 cut(s)	367, 506, 611, 1001, 1842, 2972
FriOI	GRGCYC	2 cut(s)	192, 944
FspBI	CTAG	4 cut(s)	398, 2270, 2375, 3103
GlaI	GCGC	4 cut(s)	23, 1554, 1620, 1968
GsuI	CTGGAG	4 cut(s)	633, 915, 1599, 1724
HaeII	RGCGCY	2 cut(s)	1556, 1970
HaeIII	GGCC	9 cut(s)	246, 380, 540, 744, 858, 1206, 1320, 2112, 2589
HapII	CCGG	2 cut(s)	2078, 2543
HgaI	GACGC	3 cut(s)	37, 737, 2694
HhaI	GCGC	4 cut(s)	24, 1555, 1621, 1969
Hin1I	GRCGYC	3 cut(s)	48, 729, 1967
Hin6I	GCGC	4 cut(s)	22, 1553, 1619, 1967
HinP1I	GCGC	4 cut(s)	22, 1553, 1619, 1967
HincII	GTYRAC	5 cut(s)	16, 1334, 1651, 2415, 2724
HindII	GTYRAC	5 cut(s)	16, 1334, 1651, 2415, 2724
HindIII	AAGCTT	5 cut(s)	182, 1274, 1445, 2457, 2565
HpaII	CCGG	2 cut(s)	2078, 2543
HphI	GGTGA	7 cut(s)	235, 460, 1201, 1394, 1708, 1778, 2207
Hpy99I	CGWCG	3 cut(s)	20, 86, 743
HpyAV	CCTTC	9 cut(s)	4, 256, 283, 697, 1241, 1913, 1993, 2116, 2277
HpyCH4III	ACNGT	8 cut(s)	160, 418, 835, 1234, 1496, 1810, 2231, 2658
HpyCH4IV	ACGT	3 cut(s)	97, 211, 3043
HpyF10VI	GCNNNNNNNGC	5 cut(s)	845, 905, 1120, 1282, 2921
HpySE526I	ACGT	3 cut(s)	97, 211, 3043
Hsp92I	GRCGYC	3 cut(s)	48, 729, 1967
HspAI	GCGC	4 cut(s)	22, 1553, 1619, 1967
KasI	GGCGCC	1 cut(s)	1966
Ksp22I	TGATCA	1 cut(s)	1768
LguI	GCTCTTC	1 cut(s)	1287
LmnI	GCTCC	9 cut(s)	71, 896, 1618, 1936, 2091, 2161, 2371, 3001, 3068
Lsp1109I	GCAGC	9 cut(s)	835, 859, 892, 1096, 1153, 1156, 1978, 2653, 2782
MaeI	CTAG	4 cut(s)	398, 2270, 2375, 3103
MaeII	ACGT	3 cut(s)	97, 211, 3043
MbiI	CCGCTC	1 cut(s)	66
MfeI	CAATTG	2 cut(s)	1286, 2850
MflI	RGATCY	8 cut(s)	706, 2038, 2350, 2497, 2518, 2679, 2782, 2822
MhlI	GDGCHC	3 cut(s)	192, 944, 1834
Mly113I	GGCGCC	1 cut(s)	1967
MlyI	GAGTC	4 cut(s)	662, 695, 1664, 2387
MmeI	TCCRAC	2 cut(s)	1062, 3102
Mph1103I	ATGCAT	2 cut(s)	994, 2263
MroXI	GAANNNNTTC	2 cut(s)	2512, 2538
MseI	TTAA	9 cut(s)	660, 749, 1236, 1449, 1742, 2408, 2834, 2870, 2901
MslI	CAYNNNNRTG	5 cut(s)	488, 1122, 1694, 1860, 2064
MspA1I	CMGCKG	2 cut(s)	872, 1973
MspI	CCGG	2 cut(s)	2078, 2543
MspR9I	CCNGG	4 cut(s)	530, 776, 1399, 1703
MunI	CAATTG	2 cut(s)	1286, 2850
MvaI	CCWGG	4 cut(s)	530, 776, 1399, 1703
MwoI	GCNNNNNNNGC	5 cut(s)	845, 905, 1120, 1282, 2921
NarI	GGCGCC	1 cut(s)	1967
NcoI	CCATGG	2 cut(s)	483, 1778
NdeI	CATATG	2 cut(s)	2487, 2557
NlaIV	GGNNCC	4 cut(s)	245, 1968, 2757, 3064
NsiI	ATGCAT	2 cut(s)	994, 2263
NspI	RCATGY	2 cut(s)	588, 2208
NspV	TTCGAA	4 cut(s)	511, 966, 1843, 2506
PagI	TCATGA	1 cut(s)	2625
PaqCI	CACCTGC	1 cut(s)	211
PciI	ACATGT	1 cut(s)	2204
PciSI	GCTCTTC	1 cut(s)	1287
PcsI	WCGNNNNNNNCGW	3 cut(s)	735, 1840, 2071
PdmI	GAANNNNTTC	2 cut(s)	2512, 2538
PfeI	GAWTC	8 cut(s)	112, 323, 518, 1361, 1394, 2403, 2716, 2864
PflMI	CCANNNNNTGG	3 cut(s)	2053, 2320, 2960
PleI	GAGTC	4 cut(s)	662, 694, 1663, 2386
PluTI	GGCGCC	1 cut(s)	1970
PmaCI	CACGTG	1 cut(s)	98
PmlI	CACGTG	1 cut(s)	98
PpsI	GAGTC	4 cut(s)	662, 694, 1663, 2386
Ppu21I	YACGTR	1 cut(s)	98
PscI	ACATGT	1 cut(s)	2204
PsiI	TTATAA	2 cut(s)	1002, 2663
Psp124BI	GAGCTC	1 cut(s)	192
Psp6I	CCWGG	4 cut(s)	528, 774, 1397, 1701
PspCI	CACGTG	1 cut(s)	98
PspEI	GGTNACC	2 cut(s)	1382, 1455
PspGI	CCWGG	4 cut(s)	528, 774, 1397, 1701
PspN4I	GGNNCC	4 cut(s)	245, 1968, 2757, 3064
PspPI	GGNCC	7 cut(s)	244, 743, 1205, 1319, 1643, 2122, 2212
PstI	CTGCAG	2 cut(s)	910, 2937
PsuI	RGATCY	8 cut(s)	706, 2038, 2350, 2497, 2518, 2679, 2782, 2822
PvuII	CAGCTG	2 cut(s)	872, 1973
RsaI	GTAC	7 cut(s)	317, 766, 832, 1106, 1252, 1913, 2696
RsaNI	GTAC	7 cut(s)	316, 765, 831, 1105, 1251, 1912, 2695
RseI	CAYNNNNRTG	5 cut(s)	488, 1122, 1694, 1860, 2064
SacI	GAGCTC	1 cut(s)	192
SalI	GTCGAC	3 cut(s)	14, 1332, 2413
SapI	GCTCTTC	1 cut(s)	1287
SaqAI	TTAA	9 cut(s)	660, 749, 1236, 1449, 1742, 2408, 2834, 2870, 2901
Sau96I	GGNCC	7 cut(s)	244, 743, 1205, 1319, 1643, 2122, 2212
ScaI	AGTACT	1 cut(s)	832
SchI	GAGTC	4 cut(s)	662, 695, 1664, 2387
ScrFI	CCNGG	4 cut(s)	530, 776, 1399, 1703
SduI	GDGCHC	3 cut(s)	192, 944, 1834
SfcI	CTRYAG	4 cut(s)	906, 1230, 1280, 2933
SfoI	GGCGCC	1 cut(s)	1968
SfuI	TTCGAA	4 cut(s)	511, 966, 1843, 2506
SinI	GGWCC	3 cut(s)	1643, 2122, 2212
SmiMI	CAYNNNNRTG	5 cut(s)	488, 1122, 1694, 1860, 2064
SmlI	CTYRAG	3 cut(s)	185, 2012, 2562
SmoI	CTYRAG	3 cut(s)	185, 2012, 2562
SsiI	CCGC	4 cut(s)	64, 297, 429, 1407
SspDI	GGCGCC	1 cut(s)	1966
SspI	AATATT	3 cut(s)	613, 810, 2690
SspMI	CTAG	4 cut(s)	398, 2270, 2375, 3103
SstI	GAGCTC	1 cut(s)	192
StyD4I	CCNGG	4 cut(s)	528, 774, 1397, 1701
StyI	CCWWGG	4 cut(s)	483, 1778, 2118, 2954
TaaI	ACNGT	8 cut(s)	160, 418, 835, 1234, 1496, 1810, 2231, 2658
TaiI	ACGT	3 cut(s)	100, 214, 3046
TaqII	GACCGA	1 cut(s)	1248
TatI	WGTACW	4 cut(s)	315, 830, 1104, 2694
TauI	GCSGC	1 cut(s)	1410
TfiI	GAWTC	8 cut(s)	112, 323, 518, 1361, 1394, 2403, 2716, 2864
Tru1I	TTAA	9 cut(s)	660, 749, 1236, 1449, 1742, 2408, 2834, 2870, 2901
Tru9I	TTAA	9 cut(s)	660, 749, 1236, 1449, 1742, 2408, 2834, 2870, 2901
TscAI	CASTG	5 cut(s)	1185, 1191, 1984, 2236, 2661
TseI	GCWGC	9 cut(s)	848, 872, 905, 1084, 1166, 1169, 1991, 2641, 2795
TspGWI	ACGGA	1 cut(s)	2120
TspRI	CASTG	5 cut(s)	1185, 1191, 1984, 2236, 2661
Van91I	CCANNNNNTGG	3 cut(s)	2053, 2320, 2960
VpaK11BI	GGWCC	3 cut(s)	1643, 2122, 2212
XapI	RAATTY	5 cut(s)	333, 968, 1579, 1713, 2150
XceI	RCATGY	2 cut(s)	588, 2208
XmiI	GTMKAC	5 cut(s)	15, 361, 1333, 2414, 2944
XmnI	GAANNNNTTC	2 cut(s)	2512, 2538
XspI	CTAG	4 cut(s)	398, 2270, 2375, 3103
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	2 cut(s)	994, 2263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.