Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005040

Physical Location & Seq

Genomic Coordinates Reverse (-)

3684117 .. 3697070

Gene Structure 12954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1028600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3075 bp

ATGAGGAAGAAGGTGGACGAACGCATCCGGACCCTAATAAGCAATGGCGTCAAAAACCGGCACCGCTCCATGTTCGTCATCGTCGGCGACAAGTCCCGTGACCAGATAGTGAATCTTCATTATATGCTAAGCAATGAAGTAAAAAAATCGCGTCCGACCGTCTTGTGGTGCTACAAGGACAAGCTTGAGCTCAGCAGTCACAAGAAAAAACGTGCTAAGCAGGTAAAAAAGATGATGCAGAGGGGGCTGCTTGATCCCGAGAAAGTTGATCCTTTCTCACTTTTTGTTGAAAGTGGGGGATTGACTTATTGCCTGTACAAGGATTCAGAAAGAATTCTTGGTAATACCTTTGGAATGTGTATACTTCAGGATTTCGAGGCCTTGACTCCAAATCTACTAGCAAGAACGATAGAGACAGTCGAGGGAGGTGGACTAGTCATATTGTTGCTTCGTTCTCTAACCTCACTCACCAACCTCTACACCATGGTTATGGATGTTCACGATAGGTTTCGAACTGAATCCCATTCTAAGGCTACTGGGCGTTTTAACGAGCGTTTCTTATTATCGCTTGCGTCCTGCAAATCATGTGTAGTCATGGATGATGAGCTGAATATTTTACCAATTTCATCTCATATGAGGTCAATTACCCCGGTTCCAGTTAAAGAGGACTCTGAAGGGATATCAGAGGCACAAACGGAATTGAAGGATTTAAAAGAGCAACTGAGTGATGCCTTTCCAGTTGGCCCTTTAATAAAGAAATGTTGTACGTTGGACCAGGGAAATGCTGTTGCTACATTTCTTGATGCAATCTTGGACAAGACACTCCGAAGTACTGTTGCCTTGCTTGCTGCTCGAGGCCGTGGAAAATCGGCTGCACTTGGTTTGGCGATTTCGGGAGCTATTGCTTCAGGGTATTCAAATATCTTTGTAACTGCACCCAGCCCTGAGAACTTAAGAACCTTGTTCGAATTTGTTTGCAAAGGTTTTGATCAACTGGACTATAAGGAACATATAGATTATGATGTACAGAAAAGTAGTGACCCTTCGTTGAAGAAAGCAACTGTACAGATCAATATATACAAGCAGCACAGACAAACAATTCAGTATATACGGCCACATGAGCATGAAAAGCTTTCCCAAGTTGAACTGTTGGTTGTTGATGAAGCAGCTGCTATTCCACTACCAGTGGTGAAGTCTTTGCTTGGTCCTTATCTGGTCTTCCTTTCATCTACTGTTAATGGCTATGAAGGTACCGGTCGGTCTTTGTCCCTAAAGCTTCTACAGCAATTGGAAGAGCAGAGCCAAACGTCTGCTAAAGGGCCTGTATCTGGTCGGCTTTTCAAAAAGATAGAACTGAAAGAATCTATCAGATATGCTTCTGGTGATCCGATTGAATCCTGGCTTCATGGTTTACTTTGCTTGGATATCACAAATTATATCCCTAAACTTAACGGGTTACCTGCACCAAGTGAGTGCGATCTGTACTACGTTAACCGTGATACACTGTTTTCATATCATAAAGATAGCGAGTTGTTCTTACAGCGAATGATGGCTCTATATGTTGCCTCCCACTACAAAAATTCTCCAAATGATTTGCAACTAATGGCTGATGCCCCAGCTCACCACTTGTTTGTACTACTTGGCCCTGTTGATGAGTCAAAAAATCAGCTTCCTGATATTTTGTGCGTCATTCAGGTATGCCTTGAGGGAAAGATTTCTCGTGACTCTGCAATGAAAAGTTTAAGCGATGGTCATCAGCCCTCTGGTGACCAGATACCATGGAAATTCTGTGAGCAATTTCAGGACACTGTTTTTCCCAGTCTCTCAGGTGCTCGGATTGTACGAATTGCTACACATCCCAGTGCCATGAAGATTGGATATGGATCACAAGCAGTGGAACTATTGACAAGGTACTATGAAGGACAGTTTGCTCCTATTTCTGAACTGGACGTTGAAGATGTTGTTGAGACTCCACCACTCAAAGTCACAGAAGCTGCTGAGAAGGTTTCATTGCTGGAAGAAAGTATAAAACCTAGAACAGATCTTCCTCATTTGCTCGTTCATCTTCGTGAAAGGCGACCAGAGAAACTCCATTATATTGGTGTCTCCTTTGGGCTTACATTGGACCTTTTCCGGTTTTGGTGGAAACATAAGTTTGCTCCATTCTATATTGGACATATTCCAAGTGCTGTGACGGGGGAGCATACATGTATGGTCCTCAAATCTTTGAAGAATGATGAACTTGAAACCAGTGATTTTCGTCCATTCTACCTAGATTTCAGGAGAAGATTTTTGAGACTATTGGGTTATAGTTTTCGTACGATGGATTATAGGCTTGCTATGAGCATCTTAGATCCTAAGATCAATTACAAGGATCTAGAACCCAAATCGTCTACCGCAGATGGATTCTTACAGTCAATCACAGACTTACTTTCACCTTATGATATGAAACGACTACAGGCCTACACCAGCAGTCTTGCTGACTTTCATATGATTTTGGATCTTGTTCCAACGCTTTCACATCTGTATTTCCAAGAGAAGCTTCCAGTGACATTGACAGGTGCCCAGGCTTCCATATTGCTCTGCATTGGCTTACAAAATCAAGATATCTCCTATATTGAGGGGTTGATGAAATTGGAAAGGCAGCAAATATTGTCTCTCTTTATAAAAGTTATGAAAAAGTTTCACAAATATCTATATGCTATTGCATCTGAGGGCCTCGAGTCAACCTTGCCTAAACCAAAAGAAACTCTCTTGGAACCTCACAAAATCTCTGTTGATGATGATCTCAATGATGCTGCAAGAAAAGTTGAGGACGGGGTGAGATCCAACACAGACGGATCATTAGATCCCAAGTTGCTTCAGCAGTATGCGATTGGGGACAGAGATGCAGACTTTAAGAATGCATTACAGAATGGCGGTGTAAACTTACCAGCTGGTGGTCTCGTTAGTGTGAAATCAAGTAGAAACAAAACGGATAAGGGAAAAGAGAGAGAGAGTCACAAGAGCGGGGAAAAGCGACGTAAAAATGAGCATGGCTCCAGCTCAAAGTCCAACAAAAAGAAGAAACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0000154 GO:0002097 GO:0002101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008080 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051391 GO:0051392 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1904812 GO:1990882 GO:1990883 GO:1990884 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DUF1726 GNAT_acetyltr_2 Helicase_RecD tRNA_bind_2

Protein Analysis

1025

Amino Acids

115.44

Weight (kDa)

8.56

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TmcA_N	PF08351	1 - 200	9.7e-20	tRNA(Met) cytidine acetyltransferase TmcA, N-terminal
NAT10_TcmA_helicase	PF05127	281 - 475	4.4e-56	RNA cytidine acetyltransferase NAT10/TcmA, helicase domain
GNAT_acetyltr_2	PF13718	515 - 744	1.6e-95	GNAT acetyltransferase 2
tRNA_bind_2	PF13725	754 - 969	1.4e-66	Possible tRNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005040)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10490 AT1G10490 AT3G57940 AT3G57940
fragaria_vesca	FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910 FvH4_1g22910
malus_domestica	MD05G1028000.v1.1 MD10G1028600.v1.1
prunus_persica	Prupe.6G169200_v2.0.a1
pyrus_communis	pycom05g02010 pycom10g01860
rosa_chinensis	RchiOBHm_Chr2g0117351
rosa_laevigata	RLG00000018344
rosa_multiflora	Rmu_co7970672.1_g000001 Rmu_sc0004436.1_g000001 Rmu_sc0013504.1_g000001
rosa_roxburghii	Rroxscaffold_2G00126550
rosa_rugosa	Rorug02G0212300 Rorug02G0212400
rosa_samantha	Rh2AG267400 Rh2BG279800 Rh2DG274500 Rh2DG293800
rosa_wichuraiana	Rw2G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2666
Acc36I	ACCTGC	2 cut(s)	211, 1468
Acc65I	GGTACC	1 cut(s)	1250
AccB1I	GGYRCC	3 cut(s)	60, 1250, 2561
AccB7I	CCANNNNNTGG	1 cut(s)	2939
AccBSI	CCGCTC	2 cut(s)	66, 3009
AccI	GTMKAC	2 cut(s)	361, 2393
AccII	CGCG	1 cut(s)	151
AccIII	TCCGGA	1 cut(s)	27
AciI	CCGC	4 cut(s)	64, 2397, 2919, 3009
AcoI	YGGCCR	1 cut(s)	1112
AcsI	RAATTY	4 cut(s)	333, 968, 1579, 1784
AcuI	CTGAAG	4 cut(s)	350, 693, 891, 2846
AcyI	GRCGYC	1 cut(s)	48
AdeI	CACNNNGTG	1 cut(s)	1469
AfiI	CCNNNNNNNGG	5 cut(s)	165, 319, 529, 1328, 2939
AflII	CTTAAG	1 cut(s)	952
AflIII	ACRYGT	1 cut(s)	2207
AgeI	ACCGGT	1 cut(s)	1253
AhlI	ACTAGT	1 cut(s)	433
AjnI	CCWGG	3 cut(s)	774, 1397, 2565
AjuI	GAANNNNNNNTTGG	4 cut(s)	321, 353, 2526, 2558
AloI	GAACNNNNNNTCC	2 cut(s)	2029, 2061
Alw21I	GWGCWC	2 cut(s)	192, 1834
Alw26I	GTCTC	7 cut(s)	407, 1826, 1961, 2110, 2290, 2661, 2948
AlwNI	CAGNNNCTG	1 cut(s)	1994
Ama87I	CYCGRG	3 cut(s)	257, 852, 2720
Aor13HI	TCCGGA	1 cut(s)	27
AoxI	GGCC	8 cut(s)	378, 742, 856, 1112, 1319, 1642, 2460, 2716
ApeKI	GCWGC	9 cut(s)	247, 848, 872, 1084, 1166, 1169, 1994, 2644, 2798
ApoI	RAATTY	4 cut(s)	333, 968, 1579, 1784
AsiGI	ACCGGT	1 cut(s)	1253
Asp700I	GAANNNNTTC	2 cut(s)	333, 1714
Asp718I	GGTACC	1 cut(s)	1250
AspS9I	GGNCC	9 cut(s)	30, 743, 772, 1205, 1319, 1643, 2125, 2215, 2716
AsuC2I	CCSGG	1 cut(s)	650
AsuHPI	GGTGA	7 cut(s)	460, 1201, 1394, 1613, 1778, 2427, 2833
AsuII	TTCGAA	2 cut(s)	511, 966
AvaI	CYCGRG	3 cut(s)	257, 852, 2720
AvaII	GGWCC	5 cut(s)	30, 772, 1205, 2125, 2215
BaeGI	GKGCMC	1 cut(s)	2566
BanI	GGYRCC	3 cut(s)	60, 1250, 2561
BanII	GRGCYC	1 cut(s)	192
BarI	GAAGNNNNNNTAC	2 cut(s)	1027, 1059
BauI	CACGAG	1 cut(s)	1719
BbsI	GAAGAC	1 cut(s)	1210
Bbv12I	GWGCWC	2 cut(s)	192, 1834
BbvI	GCAGC	9 cut(s)	234, 835, 859, 1096, 1156, 1178, 1981, 2656, 2785
BccI	CCATC	4 cut(s)	1543, 1742, 2317, 2396
BceAI	ACGGC	2 cut(s)	843, 1127
BciT130I	CCWGG	3 cut(s)	776, 1399, 2567
BclI	TGATCA	1 cut(s)	988
BcnI	CCSGG	1 cut(s)	650
BcoDI	GTCTC	7 cut(s)	407, 1826, 1961, 2110, 2290, 2661, 2948
BcuI	ACTAGT	1 cut(s)	433
BfaI	CTAG	5 cut(s)	398, 434, 2034, 2273, 2378
BfmI	CTRYAG	2 cut(s)	1280, 2456
BfrI	CTTAAG	1 cut(s)	952
BfuAI	ACCTGC	2 cut(s)	211, 1468
BglII	AGATCT	1 cut(s)	2041
BisI	GCNGC	9 cut(s)	248, 849, 873, 1085, 1167, 1170, 1995, 2645, 2799
BlpI	GCTNAGC	3 cut(s)	128, 191, 216
BlsI	GCNGC	9 cut(s)	249, 850, 874, 1086, 1168, 1171, 1996, 2646, 2800
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	4 cut(s)	650, 776, 1399, 2567
Bme18I	GGWCC	5 cut(s)	30, 772, 1205, 2125, 2215
BmeT110I	CYCGRG	3 cut(s)	257, 852, 2720
BmgT120I	GGNCC	9 cut(s)	30, 743, 772, 1205, 1319, 1643, 2125, 2215, 2716
BmiI	GGNNCC	7 cut(s)	32, 62, 654, 1252, 2563, 2760, 3040
BmrFI	CCNGG	4 cut(s)	650, 776, 1399, 2567
BmrI	ACTGGG	3 cut(s)	546, 1812, 1854
BmsI	GCATC	9 cut(s)	33, 225, 718, 793, 1600, 2355, 2717, 2785, 2878
BmuI	ACTGGG	3 cut(s)	546, 1812, 1854
BpiI	GAAGAC	1 cut(s)	1210
BplI	GAGNNNNNCTC	2 cut(s)	3023, 3055
BpmI	CTGGAG	1 cut(s)	3025
Bpu1102I	GCTNAGC	3 cut(s)	128, 191, 216
Bpu14I	TTCGAA	2 cut(s)	511, 966
BpuEI	CTTGAG	2 cut(s)	206, 1724
BpuMI	CCSGG	1 cut(s)	650
BsaBI	GATNNNNATC	3 cut(s)	1752, 1882, 2784
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	1 cut(s)	2948
BsaJI	CCNNGG	6 cut(s)	483, 648, 775, 859, 1778, 2565
BsaWI	WCCGGW	3 cut(s)	27, 1253, 2133
Bsc4I	CCNNNNNNNGG	5 cut(s)	165, 319, 529, 1328, 2939
Bse118I	RCCGGY	2 cut(s)	57, 1253
Bse3DI	GCAATG	4 cut(s)	49, 139, 1737, 2009
Bse8I	GATNNNNATC	3 cut(s)	1752, 1882, 2784
BseAI	TCCGGA	1 cut(s)	27
BseBI	CCWGG	3 cut(s)	776, 1399, 2567
BseDI	CCNNGG	6 cut(s)	483, 648, 775, 859, 1778, 2565
BseGI	GGATG	4 cut(s)	24, 499, 604, 1855
BseJI	GATNNNNATC	3 cut(s)	1752, 1882, 2784
BseLI	CCNNNNNNNGG	5 cut(s)	165, 319, 529, 1328, 2939
BseMI	GCAATG	4 cut(s)	49, 139, 1737, 2009
BseMII	CTCAG	6 cut(s)	205, 713, 936, 1839, 1989, 2703
BseSI	GKGCMC	1 cut(s)	2566
BseXI	GCAGC	9 cut(s)	234, 835, 859, 1096, 1156, 1178, 1981, 2656, 2785
BseYI	CCCAGC	2 cut(s)	938, 1615
BsgI	GTGCAG	3 cut(s)	858, 918, 1446
Bsh1236I	CGCG	1 cut(s)	151
Bsh1285I	CGRYCG	2 cut(s)	159, 1258
BshFI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2462, 2718
BshNI	GGYRCC	3 cut(s)	60, 1250, 2561
BshTI	ACCGGT	1 cut(s)	1253
BsiEI	CGRYCG	2 cut(s)	159, 1258
BsiHKAI	GWGCWC	2 cut(s)	192, 1834
BsiHKCI	CYCGRG	3 cut(s)	257, 852, 2720
BsiSI	CCGG	5 cut(s)	28, 58, 650, 1254, 2134
BsiWI	CGTACG	1 cut(s)	2318
BslFI	GGGAC	3 cut(s)	79, 1252, 2894
BslI	CCNNNNNNNGG	5 cut(s)	165, 319, 529, 1328, 2939
BsmAI	GTCTC	7 cut(s)	407, 1826, 1961, 2110, 2290, 2661, 2948
BsmFI	GGGAC	3 cut(s)	79, 1252, 2894
BsmI	GAATGC	1 cut(s)	2908
BsnI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2462, 2718
Bso31I	GGTCTC	1 cut(s)	2948
BsoBI	CYCGRG	3 cut(s)	257, 852, 2720
Bsp119I	TTCGAA	2 cut(s)	511, 966
Bsp1286I	GDGCHC	3 cut(s)	192, 1834, 2566
Bsp13I	TCCGGA	1 cut(s)	27
Bsp1407I	TGTACA	3 cut(s)	315, 1024, 1063
Bsp1720I	GCTNAGC	3 cut(s)	128, 191, 216
Bsp19I	CCATGG	2 cut(s)	483, 1778
BspACI	CCGC	4 cut(s)	64, 2397, 2919, 3009
BspANI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2462, 2718
BspCNI	CTCAG	6 cut(s)	204, 714, 937, 1838, 1990, 2704
BspEI	TCCGGA	1 cut(s)	27
BspFNI	CGCG	1 cut(s)	151
BspLI	GGNNCC	7 cut(s)	32, 62, 654, 1252, 2563, 2760, 3040
BspMI	ACCTGC	2 cut(s)	211, 1468
BspQI	GCTCTTC	1 cut(s)	1287
BspT104I	TTCGAA	2 cut(s)	511, 966
BspT107I	GGYRCC	3 cut(s)	60, 1250, 2561
BspTI	CTTAAG	1 cut(s)	952
BspTNI	GGTCTC	1 cut(s)	2948
BsrBI	CCGCTC	2 cut(s)	66, 3009
BsrDI	GCAATG	4 cut(s)	49, 139, 1737, 2009
BsrFI	RCCGGY	2 cut(s)	57, 1253
BsrGI	TGTACA	3 cut(s)	315, 1024, 1063
BssAI	RCCGGY	2 cut(s)	57, 1253
BssECI	CCNNGG	6 cut(s)	483, 648, 775, 859, 1778, 2565
BssNAI	GTATAC	1 cut(s)	362
BssNI	GRCGYC	1 cut(s)	48
BssSI	CACGAG	1 cut(s)	1719
BssT1I	CCWWGG	2 cut(s)	483, 1778
Bst1107I	GTATAC	1 cut(s)	362
Bst2BI	CACGAG	1 cut(s)	1719
Bst2UI	CCWGG	3 cut(s)	776, 1399, 2567
Bst6I	CTCTTC	1 cut(s)	1287
BstACI	GRCGYC	1 cut(s)	48
BstAFI	CTTAAG	1 cut(s)	952
BstAUI	TGTACA	3 cut(s)	315, 1024, 1063
BstBI	TTCGAA	2 cut(s)	511, 966
BstC8I	GCNNGC	3 cut(s)	570, 846, 2337
BstDSI	CCRYGG	3 cut(s)	483, 859, 1778
BstEII	GGTNACC	2 cut(s)	1455, 1766
BstENI	CCTNNNNNAGG	1 cut(s)	317
BstF5I	GGATG	4 cut(s)	24, 499, 604, 1855
BstFNI	CGCG	1 cut(s)	151
BstMAI	GTCTC	7 cut(s)	407, 1826, 1961, 2110, 2290, 2661, 2948
BstMCI	CGRYCG	2 cut(s)	159, 1258
BstMWI	GCNNNNNNNGC	5 cut(s)	244, 845, 1120, 1129, 1282
BstNI	CCWGG	3 cut(s)	776, 1399, 2567
BstNSI	RCATGY	1 cut(s)	2211
BstPI	GGTNACC	2 cut(s)	1455, 1766
BstSCI	CCNGG	4 cut(s)	648, 774, 1397, 2565
BstSFI	CTRYAG	2 cut(s)	1280, 2456
BstSLI	GKGCMC	1 cut(s)	2566
BstUI	CGCG	1 cut(s)	151
BstV1I	GCAGC	9 cut(s)	234, 835, 859, 1096, 1156, 1178, 1981, 2656, 2785
BstV2I	GAAGAC	1 cut(s)	1210
BstX2I	RGATCY	6 cut(s)	2041, 2353, 2374, 2500, 2825, 2848
BstXI	CCANNNNNNTGG	2 cut(s)	490, 2099
BstYI	RGATCY	6 cut(s)	2041, 2353, 2374, 2500, 2825, 2848
BstZ17I	GTATAC	1 cut(s)	362
BsuRI	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2462, 2718
BtgI	CCRYGG	3 cut(s)	483, 859, 1778
BtgZI	GCGATG	1 cut(s)	1761
BtsCI	GGATG	4 cut(s)	24, 499, 604, 1855
BtsI	GCAGTG	1 cut(s)	1899
BtsIMutI	CAGTG	7 cut(s)	1191, 1502, 1806, 1867, 1899, 2257, 2553
BveI	ACCTGC	2 cut(s)	211, 1468
Cac8I	GCNNGC	3 cut(s)	570, 846, 2337
CaiI	CAGNNNCTG	1 cut(s)	1994
Cfr10I	RCCGGY	2 cut(s)	57, 1253
Cfr13I	GGNCC	9 cut(s)	30, 743, 772, 1205, 1319, 1643, 2125, 2215, 2716
CseI	GACGC	4 cut(s)	37, 140, 561, 1675
CspAI	ACCGGT	1 cut(s)	1253
DraI	TTTAAA	1 cut(s)	711
DraIII	CACNNNGTG	1 cut(s)	1469
EaeI	YGGCCR	1 cut(s)	1112
Eam1104I	CTCTTC	1 cut(s)	1287
EarI	CTCTTC	1 cut(s)	1287
Ecl136II	GAGCTC	1 cut(s)	190
Eco130I	CCWWGG	2 cut(s)	483, 1778
Eco147I	AGGCCT	2 cut(s)	380, 2462
Eco24I	GRGCYC	1 cut(s)	192
Eco31I	GGTCTC	1 cut(s)	2948
Eco32I	GATATC	3 cut(s)	681, 1426, 2608
Eco47I	GGWCC	5 cut(s)	30, 772, 1205, 2125, 2215
Eco53kI	GAGCTC	1 cut(s)	190
Eco57I	CTGAAG	4 cut(s)	350, 693, 891, 2846
Eco88I	CYCGRG	3 cut(s)	257, 852, 2720
Eco91I	GGTNACC	2 cut(s)	1455, 1766
EcoICRI	GAGCTC	1 cut(s)	190
EcoNI	CCTNNNNNAGG	1 cut(s)	317
EcoO109I	RGGNCCY	2 cut(s)	1319, 2716
EcoO65I	GGTNACC	2 cut(s)	1455, 1766
EcoRI	GAATTC	1 cut(s)	333
EcoRII	CCWGG	3 cut(s)	774, 1397, 2565
EcoRV	GATATC	3 cut(s)	681, 1426, 2608
EcoT14I	CCWWGG	2 cut(s)	483, 1778
EcoT22I	ATGCAT	1 cut(s)	2908
EcoT38I	GRGCYC	1 cut(s)	192
ErhI	CCWWGG	2 cut(s)	483, 1778
FalI	AAGNNNNNCTT	2 cut(s)	2526, 2558
FaqI	GGGAC	3 cut(s)	79, 1252, 2894
FauI	CCCGC	1 cut(s)	3002
FauNDI	CATATG	2 cut(s)	633, 2490
FbaI	TGATCA	1 cut(s)	988
FblI	GTMKAC	2 cut(s)	361, 2393
Fnu4HI	GCNGC	9 cut(s)	248, 849, 873, 1085, 1167, 1170, 1995, 2645, 2799
FokI	GGATG	4 cut(s)	11, 506, 611, 1842
FriOI	GRGCYC	1 cut(s)	192
Fsp4HI	GCNGC	9 cut(s)	248, 849, 873, 1085, 1167, 1170, 1995, 2645, 2799
FspBI	CTAG	5 cut(s)	398, 434, 2034, 2273, 2378
GluI	GCNGC	9 cut(s)	248, 849, 873, 1085, 1167, 1170, 1995, 2645, 2799
GsaI	CCCAGC	2 cut(s)	942, 1619
GsuI	CTGGAG	1 cut(s)	3025
HaeIII	GGCC	8 cut(s)	380, 744, 858, 1114, 1321, 1644, 2462, 2718
HapII	CCGG	5 cut(s)	28, 58, 650, 1254, 2134
HgaI	GACGC	4 cut(s)	37, 140, 561, 1675
Hin1I	GRCGYC	1 cut(s)	48
HincII	GTYRAC	2 cut(s)	1492, 2727
HindII	GTYRAC	2 cut(s)	1492, 2727
HindIII	AAGCTT	4 cut(s)	182, 1130, 1274, 2540
HpaI	GTTAAC	1 cut(s)	1492
HpaII	CCGG	5 cut(s)	28, 58, 650, 1254, 2134
HphI	GGTGA	7 cut(s)	460, 1201, 1394, 1613, 1778, 2427, 2833
Hpy166II	GTNNAC	9 cut(s)	16, 362, 431, 499, 1412, 1492, 2394, 2727, 2926
Hpy8I	GTNNAC	9 cut(s)	16, 362, 431, 499, 1412, 1492, 2394, 2727, 2926
Hpy99I	CGWCG	2 cut(s)	86, 3024
HpyAV	CCTTC	7 cut(s)	4, 668, 697, 1053, 1241, 1913, 1996
HpyCH4IV	ACGT	6 cut(s)	211, 767, 1307, 1488, 1950, 3022
HpyF10VI	GCNNNNNNNGC	5 cut(s)	244, 845, 1120, 1129, 1282
HpySE526I	ACGT	6 cut(s)	211, 767, 1307, 1488, 1950, 3022
Hsp92I	GRCGYC	1 cut(s)	48
Kpn2I	TCCGGA	1 cut(s)	27
KpnI	GGTACC	1 cut(s)	1254
Ksp22I	TGATCA	1 cut(s)	988
KspAI	GTTAAC	1 cut(s)	1492
LguI	GCTCTTC	1 cut(s)	1287
LmnI	GCTCC	6 cut(s)	71, 896, 1936, 2164, 2200, 3044
Lsp1109I	GCAGC	9 cut(s)	234, 835, 859, 1096, 1156, 1178, 1981, 2656, 2785
LweI	GCATC	9 cut(s)	33, 225, 718, 793, 1600, 2355, 2717, 2785, 2878
MaeI	CTAG	5 cut(s)	398, 434, 2034, 2273, 2378
MaeII	ACGT	6 cut(s)	211, 767, 1307, 1488, 1950, 3022
MbiI	CCGCTC	2 cut(s)	66, 3009
MfeI	CAATTG	1 cut(s)	1286
MflI	RGATCY	6 cut(s)	2041, 2353, 2374, 2500, 2825, 2848
MhlI	GDGCHC	3 cut(s)	192, 1834, 2566
MlyI	GAGTC	7 cut(s)	379, 662, 1664, 1718, 1963, 2732, 3007
MmeI	TCCRAC	4 cut(s)	179, 750, 2534, 2853
Mph1103I	ATGCAT	1 cut(s)	2908
MroI	TCCGGA	1 cut(s)	27
MroXI	GAANNNNTTC	2 cut(s)	333, 1714
MslI	CAYNNNNRTG	4 cut(s)	488, 1122, 1860, 2067
MspA1I	CMGCKG	2 cut(s)	1169, 2936
MspCI	CTTAAG	1 cut(s)	952
MspI	CCGG	5 cut(s)	28, 58, 650, 1254, 2134
MspR9I	CCNGG	4 cut(s)	650, 776, 1399, 2567
MunI	CAATTG	1 cut(s)	1286
Mva1269I	GAATGC	1 cut(s)	2908
MvaI	CCWGG	3 cut(s)	776, 1399, 2567
MvnI	CGCG	1 cut(s)	151
MwoI	GCNNNNNNNGC	5 cut(s)	244, 845, 1120, 1129, 1282
NciI	CCSGG	1 cut(s)	650
NcoI	CCATGG	2 cut(s)	483, 1778
NdeI	CATATG	2 cut(s)	633, 2490
NlaIV	GGNNCC	7 cut(s)	32, 62, 654, 1252, 2563, 2760, 3040
NmuCI	GTSAC	9 cut(s)	98, 197, 1037, 1721, 1766, 1984, 2191, 2548, 2999
NsiI	ATGCAT	1 cut(s)	2908
NspI	RCATGY	1 cut(s)	2211
NspV	TTCGAA	2 cut(s)	511, 966
PaeR7I	CTCGAG	2 cut(s)	852, 2720
PceI	AGGCCT	2 cut(s)	380, 2462
PciI	ACATGT	1 cut(s)	2207
PciSI	GCTCTTC	1 cut(s)	1287
PcsI	WCGNNNNNNNCGW	2 cut(s)	1840, 2074
PctI	GAATGC	1 cut(s)	2908
PdmI	GAANNNNTTC	2 cut(s)	333, 1714
PfeI	GAWTC	6 cut(s)	112, 323, 518, 1361, 1394, 2406
Pfl23II	CGTACG	1 cut(s)	2318
PflMI	CCANNNNNTGG	1 cut(s)	2939
PinAI	ACCGGT	1 cut(s)	1253
PkrI	GCNGC	9 cut(s)	249, 850, 874, 1086, 1168, 1171, 1996, 2646, 2800
PleI	GAGTC	7 cut(s)	379, 662, 1663, 1718, 1963, 2731, 3006
PpsI	GAGTC	7 cut(s)	379, 662, 1663, 1718, 1963, 2731, 3006
PscI	ACATGT	1 cut(s)	2207
PsiI	TTATAA	1 cut(s)	2666
Psp124BI	GAGCTC	1 cut(s)	192
Psp6I	CCWGG	3 cut(s)	774, 1397, 2565
PspEI	GGTNACC	2 cut(s)	1455, 1766
PspFI	CCCAGC	2 cut(s)	938, 1615
PspGI	CCWGG	3 cut(s)	774, 1397, 2565
PspLI	CGTACG	1 cut(s)	2318
PspN4I	GGNNCC	7 cut(s)	32, 62, 654, 1252, 2563, 2760, 3040
PspPI	GGNCC	9 cut(s)	30, 743, 772, 1205, 1319, 1643, 2125, 2215, 2716
PspXI	VCTCGAGB	2 cut(s)	852, 2720
PstNI	CAGNNNCTG	1 cut(s)	1994
PsuI	RGATCY	6 cut(s)	2041, 2353, 2374, 2500, 2825, 2848
PvuII	CAGCTG	2 cut(s)	1169, 2936
RseI	CAYNNNNRTG	4 cut(s)	488, 1122, 1860, 2067
SacI	GAGCTC	1 cut(s)	192
SapI	GCTCTTC	1 cut(s)	1287
SatI	GCNGC	9 cut(s)	248, 849, 873, 1085, 1167, 1170, 1995, 2645, 2799
Sau96I	GGNCC	9 cut(s)	30, 743, 772, 1205, 1319, 1643, 2125, 2215, 2716
ScaI	AGTACT	1 cut(s)	832
SchI	GAGTC	7 cut(s)	379, 662, 1664, 1718, 1963, 2732, 3007
ScrFI	CCNGG	4 cut(s)	650, 776, 1399, 2567
SduI	GDGCHC	3 cut(s)	192, 1834, 2566
SfaNI	GCATC	9 cut(s)	33, 225, 718, 793, 1600, 2355, 2717, 2785, 2878
SfcI	CTRYAG	2 cut(s)	1280, 2456
Sfr274I	CTCGAG	2 cut(s)	852, 2720
SfuI	TTCGAA	2 cut(s)	511, 966
SinI	GGWCC	5 cut(s)	30, 772, 1205, 2125, 2215
SlaI	CTCGAG	2 cut(s)	852, 2720
SmiMI	CAYNNNNRTG	4 cut(s)	488, 1122, 1860, 2067
SmlI	CTYRAG	5 cut(s)	185, 852, 952, 1703, 2720
SmoI	CTYRAG	5 cut(s)	185, 852, 952, 1703, 2720
SpeI	ACTAGT	1 cut(s)	433
SseBI	AGGCCT	2 cut(s)	380, 2462
SsiI	CCGC	4 cut(s)	64, 2397, 2919, 3009
SspI	AATATT	2 cut(s)	613, 2652
SspMI	CTAG	5 cut(s)	398, 434, 2034, 2273, 2378
SstI	GAGCTC	1 cut(s)	192
StuI	AGGCCT	2 cut(s)	380, 2462
StyD4I	CCNGG	4 cut(s)	648, 774, 1397, 2565
StyI	CCWWGG	2 cut(s)	483, 1778
TaiI	ACGT	6 cut(s)	214, 770, 1310, 1491, 1953, 3025
TaqI	TCGA	6 cut(s)	375, 420, 511, 853, 966, 2721
TaqII	GACCGA	1 cut(s)	1248
TatI	WGTACW	6 cut(s)	315, 830, 1024, 1063, 1482, 1633
TfiI	GAWTC	6 cut(s)	112, 323, 518, 1361, 1394, 2406
TscAI	CASTG	7 cut(s)	1191, 1509, 1813, 1867, 1899, 2257, 2553
TseFI	GTSAC	9 cut(s)	98, 197, 1037, 1721, 1766, 1984, 2191, 2548, 2999
TseI	GCWGC	9 cut(s)	247, 848, 872, 1084, 1166, 1169, 1994, 2644, 2798
Tsp45I	GTSAC	9 cut(s)	98, 197, 1037, 1721, 1766, 1984, 2191, 2548, 2999
TspGWI	ACGGA	3 cut(s)	710, 2853, 2990
TspRI	CASTG	7 cut(s)	1191, 1509, 1813, 1867, 1899, 2257, 2553
Van91I	CCANNNNNTGG	1 cut(s)	2939
Vha464I	CTTAAG	1 cut(s)	952
VpaK11BI	GGWCC	5 cut(s)	30, 772, 1205, 2125, 2215
XagI	CCTNNNNNAGG	1 cut(s)	317
XapI	RAATTY	4 cut(s)	333, 968, 1579, 1784
XbaI	TCTAGA	1 cut(s)	2377
XceI	RCATGY	1 cut(s)	2211
XhoI	CTCGAG	2 cut(s)	852, 2720
XmiI	GTMKAC	2 cut(s)	361, 2393
XmnI	GAANNNNTTC	2 cut(s)	333, 1714
XspI	CTAG	5 cut(s)	398, 434, 2034, 2273, 2378
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	2908

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.