MD05G1067500.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011365
Physical Location & Seq
Forward (+)
12702446 .. 12709429
6984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1067500.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGGCTCAGCCTCTCGTGAAGAAGGACGACGATCGTGACGATGAAGAGTACTCGCCGTTTCTGGGGATTGAGAAGGGTGCTGTTCTTCAGGAAGCGAGAGTTTTCAATGATCCTCAACTCGATGCTAGAAGATGCTCCCAGGTTATCACAAAACTTTTGTACCTACTGAACCAAGGGGAAAGCTTTACAAAGGTTGAAGCTACAGAAGTTTTCTTTTCTGTGACTAAGCTTTTCCAATCAAGAGATATAGGATTGAGGAGAATGGTTTACCTGATCATAAAAGAATTGTCCCCCTCTGCAGATGAGGTTATTATTGTGACAAGCTCCCTGATGAAGGATATGAATAGTAAAACTGACATGTATCGGGCAAATGCCATCCGTGTGCTCTGTCGGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATCGTTGATAAGAATCCAGTGGTTGCGAGTGCAGCTCTGGTCAGTGGGATCCATCTTCTCCAGACTAACCCAGAGATTGTCAAAAGGTGGAGTAATGAGGTTCAGGAATCTGTCCAGTCAAGAGCAGCCCTTGTACAATTCCATGCACTGGCTTTACTTCATCAGATACGACAGAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGGACTGTCCGCTCTCCATTGGCCCAGTGCCTGTTGATTCGTTATACTAGTCAGGTTATCCGCGAGTCTGGAAATGTACAAACAGGGGACCGTCCCTTTTACGATTATCTTGAGGGTTGTCTTCGCCACAAAGTAGAGATGGTGATTTTTGAGGCTGCTAGAGCAATTACAGAGCTCCATGGTGTGACCCCCAGAGAACTTACTCCAGCAATCACTGTTCTGCAGCTATTTCTAAGTTCTTCCAAACCAGTTTTAAGATTTGCTGCTGTCCGCACCTTGAATAAGGCATCTTTCTTCTCCTTTTACTCTACTACACAATGTGTGGCAATGACACATCCAGTGCCTGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCTATCACAACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGATTGATGAAGCAGATAACTAATTTCATGTCAGATATTGCTGATGAGTTCAAAATTGTTGTTGTGGAAGCCATAAGATCGTTGTGTTTGAAATTTCCATTGAAGTTCAGAGCTTTGATGAACTTTTTGAGCAACATTCTCAGAGAAGAAGGTGGATTTGAGTACAAAAAGGCAATTGTTGATTCAATTGTGATTCTCATAAGAGATATTCCTGATGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATCGAAGATTGTGAATTCACCTATCTTTCTACACAGATACTCCACTTTTTGGGTATTGAAGGGCCAAAAACATCTGATCCAAGCAAATATATACGGTACATTTATAATCGAGTACATCTTGAGAATGCCACTGTTCGTGCCAGTGCGGTTAGTACATTGGCAAAATTTGGTGCTTTGGTCGATTCATTGAAGCCACGAGTGTTTATTCTGCTGCGAAGATGTCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTAAATACGCTTGGCGGAGATGGTTCAGTTATTGAGACTGACAATGATGTAAAGGACTTCCTCTTTGGGTCTCTGGATGTCCCACTTGTCAATCTGGAGACAAGTTTGAAGAATTATGAGGCTTCGGAAGAACCATTTGACATTAAATCTGTACCCAAGGAGGTTAAGTCGCAGCCACTTGCTGAGAAGAAAGCCCAGAGTAAAAAGCCAACTGGTCTTGGTGCTCCTCCAACTGGCCCTGCATCCACAGTTGATGCTTATGAGACGTTGCTTTCCTCTATTCCGGAGTTTTCAAACTTTGGGAAACTCTTCAAGTCCTCAGCACCTGTGGAGTTAACAGAGCCAGAAACCGAATATGCAGTTAATGTTGTCAAGCACATTTTTGATAGCCATGTTGTGTTCCAGTACAACTGCACCAATACCATTCCCGAGCAATTACTGGAAGATGTCATTGTTGTGGTGGATGCTTCAGAAGCTGAGGAATTTTCTGAAGTGGCATCCAGGTCTCTCAGGTCTCTTCCGTATGATACACCTGGACAAACCTTTTTGGCATTTGAGAAGCCAGAAGGCATCCCTGCAGTTGGAAAATTTACAAACTTGTTGAGGTTCATTGTTAAAGAGGTTGATCCAACCACTGGTGAGGCAGAGGAAGATGGTGTGGAAGATGAATACCAGCTGGAAGATCTTGAGGTTGTCGCTGCTGATTACATTTTGAAGGTTCCAGTCTTCAATTTCAAGAATGCTTGGGAAAGCATGGGCGCGGATTTTGAGCGGGTAGATGAATATGGCCTGGGGCCAAGGGAGAACTTGACTGAAGCTGTTAATGCTGTCATCAGCCTCCTTGGAATGCAACCCTGTGAGGGCACAGAGGCCGTTGCGAGCAACTCAAGGTCACACACTTGTGTATTGTCCGGTGTATACATAGGCAATGTGAAGGTCCTTGTGCGGTTGTTATTTGGAATTGATAGTTCATCAAAGGAGGTTGCGATGAAACTCACTGTGAGATCTGAGGACGGAGCTGTTAGTGATGCGATTCATGAGATTGTAGCCAGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

899

Amino Acids

99.92

Weight (kDa)

5.1

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 550 1.1e-133 Adaptin N terminal region
Cnd1 PF12717 122 - 217 2.4e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 632 - 777 1.5e-52 Coatomer gamma subunit appendage platform subdomain
Coatomer_g_Cpla PF16381 780 - 895 2.4e-35 Coatomer subunit gamma-1 C-terminal appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1476
AccB7I CCANNNNNTGG 3 cut(s) 592, 1420, 2273
AccBSI CCGCTC 3 cut(s) 427, 675, 2410
AccI GTMKAC 1 cut(s) 2556
AccII CGCG 2 cut(s) 726, 2399
AccIII TCCGGA 1 cut(s) 1921
AclWI GGATC 6 cut(s) 104, 487, 500, 1125, 1442, 2258
AcsI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
AcuI CTGAAG 5 cut(s) 71, 1112, 2091, 2148, 2472
AdeI CACNNNGTG 1 cut(s) 983
AfiI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1420, 1871, 2219, 2273, 2498
AflIII ACRYGT 1 cut(s) 357
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 4 cut(s) 138, 2138, 2170, 2427
AleI CACNNNNGTG 1 cut(s) 2538
Alw21I GWGCWC 3 cut(s) 387, 840, 1864
Alw26I GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
AlwI GGATC 6 cut(s) 104, 487, 500, 1125, 1442, 2258
AlwNI CAGNNNCTG 4 cut(s) 694, 1007, 1964, 2114
Ama87I CYCGRG 1 cut(s) 2066
Aor13HI TCCGGA 1 cut(s) 1921
AoxI GGCC 6 cut(s) 684, 1433, 1873, 2425, 2432, 2508
ApeKI GCWGC 8 cut(s) 476, 569, 818, 886, 926, 1582, 1810, 2336
ApoI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
ArsI GACNNNNNNTTYG 2 cut(s) 1685, 1717
Asp700I GAANNNNTTC 1 cut(s) 1946
AspLEI GCGC 1 cut(s) 2399
AspS9I GGNCC 6 cut(s) 685, 751, 1433, 1874, 2432, 2575
AsuHPI GGTGA 3 cut(s) 817, 1381, 2288
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 2 cut(s) 751, 2575
BaeGI GKGCMC 1 cut(s) 2504
BaeI ACNNNNGTAYC 2 cut(s) 143, 176
BamHI GGATCC 1 cut(s) 492
BanII GRGCYC 1 cut(s) 840
BauI CACGAG 2 cut(s) 14, 1566
BbsI GAAGAC 3 cut(s) 776, 1100, 2356
Bbv12I GWGCWC 3 cut(s) 387, 840, 1864
BbvCI CCTCAGC 2 cut(s) 1957, 2115
BbvI GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 2323
BccI CCATC 7 cut(s) 383, 395, 452, 504, 796, 1652, 2285
BceAI ACGGC 2 cut(s) 40, 2495
BciT130I CCWGG 4 cut(s) 140, 2140, 2172, 2429
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 126, 711, 822, 2695
BfmI CTRYAG 4 cut(s) 201, 297, 884, 2214
BglII AGATCT 2 cut(s) 2320, 2642
BisI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1583, 1811, 2337, 2692
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1584, 1812, 2338, 2693
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 140, 2140, 2172, 2429
Bme18I GGWCC 2 cut(s) 751, 2575
BmeT110I CYCGRG 1 cut(s) 2066
BmgT120I GGNCC 6 cut(s) 685, 751, 1433, 1874, 2432, 2575
BmiI GGNNCC 5 cut(s) 406, 494, 752, 2358, 2433
BmrFI CCNGG 4 cut(s) 140, 2140, 2172, 2429
BmrI ACTGGG 1 cut(s) 682
BmuI ACTGGG 1 cut(s) 682
BpiI GAAGAC 3 cut(s) 776, 1100, 2356
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 3 cut(s) 488, 852, 1754
Bpu10I CCTNAGC 2 cut(s) 1957, 2115
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 794, 1511, 2345, 2509
Bsa29I ATCGAT 1 cut(s) 1120
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 3 cut(s) 1713, 2148, 2157
BsaJI CCNNGG 7 cut(s) 138, 172, 841, 1794, 2428, 2435, 2479
BsaWI WCCGGW 2 cut(s) 1921, 2549
BsaXI ACNNNNNCTCC 4 cut(s) 1395, 1425, 1790, 1820
Bsc4I CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1420, 1871, 2219, 2273, 2498
Bse3DI GCAATG 3 cut(s) 996, 1062, 2572
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 1921
BseBI CCWGG 4 cut(s) 140, 2140, 2172, 2429
BseCI ATCGAT 1 cut(s) 1120
BseDI CCNNGG 7 cut(s) 138, 172, 841, 1794, 2428, 2435, 2479
BseGI GGATG 8 cut(s) 375, 406, 997, 1720, 1880, 2107, 2135, 2208
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1420, 1871, 2219, 2273, 2498
BseMI GCAATG 3 cut(s) 996, 1062, 2572
BseMII CTCAG 7 cut(s) 20, 1273, 1812, 1971, 2106, 2161, 2637
BseRI GAGGAG 2 cut(s) 271, 1854
BseSI GKGCMC 1 cut(s) 2504
BseXI GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 2323
BsgI GTGCAG 2 cut(s) 495, 2035
Bsh1236I CGCG 2 cut(s) 726, 2399
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 6 cut(s) 686, 1435, 1875, 2427, 2434, 2510
BshVI ATCGAT 1 cut(s) 1120
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 3 cut(s) 387, 840, 1864
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 2 cut(s) 1922, 2550
BslFI GGGAC 5 cut(s) 274, 679, 741, 764, 1703
BslI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1420, 1871, 2219, 2273, 2498
BsmAI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 5 cut(s) 274, 679, 741, 764, 1703
BsmI GAATGC 3 cut(s) 1501, 2383, 2490
BsnI GGCC 6 cut(s) 686, 1435, 1875, 2427, 2434, 2510
Bso31I GGTCTC 3 cut(s) 1713, 2148, 2157
BsoBI CYCGRG 1 cut(s) 2066
Bsp1286I GDGCHC 4 cut(s) 387, 840, 1864, 2504
Bsp13I TCCGGA 1 cut(s) 1921
Bsp1407I TGTACA 2 cut(s) 577, 739
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 841
BspANI GGCC 6 cut(s) 686, 1435, 1875, 2427, 2434, 2510
BspCNI CTCAG 7 cut(s) 19, 1272, 1813, 1970, 2107, 2160, 2638
BspDI ATCGAT 1 cut(s) 1120
BspEI TCCGGA 1 cut(s) 1921
BspFNI CGCG 2 cut(s) 726, 2399
BspHI TCATGA 1 cut(s) 2674
BspLI GGNNCC 5 cut(s) 406, 494, 752, 2358, 2433
BspMAI CTGCAG 3 cut(s) 301, 888, 2218
BspPI GGATC 6 cut(s) 104, 487, 500, 1125, 1442, 2258
BspTNI GGTCTC 3 cut(s) 1713, 2148, 2157
BsrBI CCGCTC 3 cut(s) 427, 675, 2410
BsrDI GCAATG 3 cut(s) 996, 1062, 2572
BsrGI TGTACA 2 cut(s) 577, 739
BssECI CCNNGG 7 cut(s) 138, 172, 841, 1794, 2428, 2435, 2479
BssNAI GTATAC 1 cut(s) 2557
BssSI CACGAG 2 cut(s) 14, 1566
BssT1I CCWWGG 5 cut(s) 172, 841, 1794, 2435, 2479
Bst1107I GTATAC 1 cut(s) 2557
Bst2BI CACGAG 2 cut(s) 14, 1566
Bst2UI CCWGG 4 cut(s) 140, 2140, 2172, 2429
Bst4CI ACNGT 9 cut(s) 626, 670, 755, 880, 1467, 1504, 1604, 1888, 2638
Bst6I CTCTTC 3 cut(s) 39, 1952, 2160
BstAUI TGTACA 2 cut(s) 577, 739
BstC8I GCNNGC 3 cut(s) 1352, 2518, 2689
BstDEI CTNAG 9 cut(s) 6, 225, 896, 1259, 1821, 1957, 2115, 2147, 2646
BstDSI CCRYGG 1 cut(s) 841
BstEII GGTNACC 1 cut(s) 646
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 8 cut(s) 375, 406, 997, 1720, 1880, 2107, 2135, 2208
BstFNI CGCG 2 cut(s) 726, 2399
BstHHI GCGC 1 cut(s) 2399
BstMAI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 476, 824, 1073, 2111, 2462
BstNI CCWGG 4 cut(s) 140, 2140, 2172, 2429
BstNSI RCATGY 1 cut(s) 361
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 4 cut(s) 138, 2138, 2170, 2427
BstSFI CTRYAG 4 cut(s) 201, 297, 884, 2214
BstSLI GKGCMC 1 cut(s) 2504
BstUI CGCG 2 cut(s) 726, 2399
BstV1I GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 2323
BstV2I GAAGAC 3 cut(s) 776, 1100, 2356
BstX2I RGATCY 3 cut(s) 492, 2320, 2642
BstYI RGATCY 3 cut(s) 492, 2320, 2642
BstZ17I GTATAC 1 cut(s) 2557
Bsu15I ATCGAT 1 cut(s) 1120
BsuRI GGCC 6 cut(s) 686, 1435, 1875, 2427, 2434, 2510
BsuTUI ATCGAT 1 cut(s) 1120
BtgI CCRYGG 1 cut(s) 841
BtgZI GCGATG 1 cut(s) 2639
BtsCI GGATG 8 cut(s) 375, 406, 997, 1720, 1880, 2107, 2135, 2208
Cac8I GCNNGC 3 cut(s) 1352, 2518, 2689
CaiI CAGNNNCTG 4 cut(s) 694, 1007, 1964, 2114
CciI TCATGA 1 cut(s) 2674
CfoI GCGC 1 cut(s) 2399
Cfr13I GGNCC 6 cut(s) 685, 751, 1433, 1874, 2432, 2575
ClaI ATCGAT 1 cut(s) 1120
CviAII CATG 7 cut(s) 358, 587, 842, 1147, 2030, 2392, 2675
DdeI CTNAG 9 cut(s) 6, 225, 896, 1259, 1821, 1957, 2115, 2147, 2646
DraIII CACNNNGTG 1 cut(s) 983
Eam1104I CTCTTC 3 cut(s) 39, 1952, 2160
EarI CTCTTC 3 cut(s) 39, 1952, 2160
EciI GGCGGA 1 cut(s) 1668
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 5 cut(s) 172, 841, 1794, 2435, 2479
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 3 cut(s) 1713, 2148, 2157
Eco47I GGWCC 2 cut(s) 751, 2575
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 5 cut(s) 71, 1112, 2091, 2148, 2472
Eco88I CYCGRG 1 cut(s) 2066
Eco91I GGTNACC 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 838
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 2575
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 4 cut(s) 138, 2138, 2170, 2427
EcoT14I CCWWGG 5 cut(s) 172, 841, 1794, 2435, 2479
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 5 cut(s) 172, 841, 1794, 2435, 2479
Esp3I CGTCTC 1 cut(s) 1895
FaeI CATG 7 cut(s) 361, 590, 845, 1150, 2033, 2395, 2678
FaqI GGGAC 5 cut(s) 274, 679, 741, 764, 1703
FatI CATG 7 cut(s) 357, 586, 841, 1146, 2029, 2391, 2674
FauI CCCGC 1 cut(s) 2403
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 1 cut(s) 2556
Fnu4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1583, 1811, 2337, 2692
FokI GGATG 8 cut(s) 362, 413, 984, 1727, 1867, 2114, 2122, 2195
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1583, 1811, 2337, 2692
FspBI CTAG 4 cut(s) 126, 711, 822, 2695
GlaI GCGC 1 cut(s) 2398
GluI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1583, 1811, 2337, 2692
GsuI CTGGAG 3 cut(s) 488, 852, 1754
HaeIII GGCC 6 cut(s) 686, 1435, 1875, 2427, 2434, 2510
HapII CCGG 2 cut(s) 1922, 2550
HhaI GCGC 1 cut(s) 2399
Hin1II CATG 7 cut(s) 361, 590, 845, 1150, 2033, 2395, 2678
Hin6I GCGC 1 cut(s) 2397
HinP1I GCGC 1 cut(s) 2397
HincII GTYRAC 1 cut(s) 1974
HindII GTYRAC 1 cut(s) 1974
HindIII AAGCTT 2 cut(s) 181, 227
HinfI GANTC 9 cut(s) 457, 551, 700, 728, 1106, 1301, 1312, 1553, 2671
HpaI GTTAAC 1 cut(s) 1974
HpaII CCGG 2 cut(s) 1922, 2550
HphI GGTGA 3 cut(s) 817, 1381, 2288
Hpy166II GTNNAC 3 cut(s) 268, 1974, 2557
Hpy8I GTNNAC 3 cut(s) 268, 1974, 2557
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 9 cut(s) 16, 67, 328, 419, 1262, 1424, 2198, 2347, 2566
HpyCH4III ACNGT 9 cut(s) 626, 670, 755, 880, 1467, 1504, 1604, 1888, 2638
HpyCH4IV ACGT 1 cut(s) 1904
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 824, 1073, 2111, 2462
HpyF3I CTNAG 9 cut(s) 6, 225, 896, 1259, 1821, 1957, 2115, 2147, 2646
HpySE526I ACGT 1 cut(s) 1904
Hsp92II CATG 7 cut(s) 361, 590, 845, 1150, 2033, 2395, 2678
HspAI GCGC 1 cut(s) 2397
Kpn2I TCCGGA 1 cut(s) 1921
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 1974
LmnI GCTCC 5 cut(s) 140, 329, 843, 1867, 2654
Lsp1109I GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 2323
MaeI CTAG 4 cut(s) 126, 711, 822, 2695
MaeII ACGT 1 cut(s) 1904
MaeIII GTNAC 7 cut(s) 35, 220, 316, 646, 847, 1009, 2529
MbiI CCGCTC 3 cut(s) 427, 675, 2410
MfeI CAATTG 2 cut(s) 1293, 1305
MflI RGATCY 3 cut(s) 492, 2320, 2642
MhlI GDGCHC 4 cut(s) 387, 840, 1864, 2504
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 4 cut(s) 371, 1892, 2200, 2291
MroI TCCGGA 1 cut(s) 1921
MroXI GAANNNNTTC 1 cut(s) 1946
MseI TTAA 7 cut(s) 917, 1782, 1803, 1973, 2001, 2253, 2460
MslI CAYNNNNRTG 5 cut(s) 380, 1028, 1362, 2093, 2538
MspA1I CMGCKG 2 cut(s) 2314, 2691
MspI CCGG 2 cut(s) 1922, 2550
MspR9I CCNGG 4 cut(s) 140, 2140, 2172, 2429
MunI CAATTG 2 cut(s) 1293, 1305
Mva1269I GAATGC 3 cut(s) 1501, 2383, 2490
MvaI CCWGG 4 cut(s) 140, 2140, 2172, 2429
MvnI CGCG 2 cut(s) 726, 2399
MwoI GCNNNNNNNGC 5 cut(s) 476, 824, 1073, 2111, 2462
NcoI CCATGG 1 cut(s) 841
NlaIII CATG 7 cut(s) 361, 590, 845, 1150, 2033, 2395, 2678
NlaIV GGNNCC 5 cut(s) 406, 494, 752, 2358, 2433
NmuCI GTSAC 6 cut(s) 35, 220, 316, 847, 1009, 2529
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 2538
PagI TCATGA 1 cut(s) 2674
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 3 cut(s) 1501, 2383, 2490
PdmI GAANNNNTTC 1 cut(s) 1946
PfeI GAWTC 8 cut(s) 457, 551, 700, 1106, 1301, 1312, 1553, 2671
PflMI CCANNNNNTGG 3 cut(s) 592, 1420, 2273
PkrI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1584, 1812, 2338, 2693
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PpuMI RGGWCCY 1 cut(s) 2575
PscI ACATGT 1 cut(s) 357
PsiI TTATAA 1 cut(s) 1476
Psp124BI GAGCTC 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 2575
Psp6I CCWGG 4 cut(s) 138, 2138, 2170, 2427
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 4 cut(s) 138, 2138, 2170, 2427
PspN4I GGNNCC 5 cut(s) 406, 494, 752, 2358, 2433
PspPI GGNCC 6 cut(s) 685, 751, 1433, 1874, 2432, 2575
PspPPI RGGWCCY 1 cut(s) 2575
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstI CTGCAG 3 cut(s) 301, 888, 2218
PstNI CAGNNNCTG 4 cut(s) 694, 1007, 1964, 2114
PsuI RGATCY 3 cut(s) 492, 2320, 2642
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 2314
RseI CAYNNNNRTG 5 cut(s) 380, 1028, 1362, 2093, 2538
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 7 cut(s) 917, 1782, 1803, 1973, 2001, 2253, 2460
SatI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1583, 1811, 2337, 2692
Sau96I GGNCC 6 cut(s) 685, 751, 1433, 1874, 2432, 2575
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 4 cut(s) 140, 2140, 2172, 2429
SduI GDGCHC 4 cut(s) 387, 840, 1864, 2504
SfcI CTRYAG 4 cut(s) 201, 297, 884, 2214
SinI GGWCC 2 cut(s) 751, 2575
SmiMI CAYNNNNRTG 5 cut(s) 380, 1028, 1362, 2093, 2538
SmlI CTYRAG 4 cut(s) 773, 1490, 2324, 2524
SmoI CTYRAG 4 cut(s) 773, 1490, 2324, 2524
SpeI ACTAGT 1 cut(s) 710
SspMI CTAG 4 cut(s) 126, 711, 822, 2695
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 4 cut(s) 138, 2138, 2170, 2427
StyI CCWWGG 5 cut(s) 172, 841, 1794, 2435, 2479
TaaI ACNGT 9 cut(s) 626, 670, 755, 880, 1467, 1504, 1604, 1888, 2638
TaiI ACGT 1 cut(s) 1907
TaqI TCGA 5 cut(s) 120, 1120, 1374, 1480, 1551
TatI WGTACW 7 cut(s) 48, 577, 739, 1281, 1483, 1523, 2043
TauI GCSGC 1 cut(s) 2694
TfiI GAWTC 8 cut(s) 457, 551, 700, 1106, 1301, 1312, 1553, 2671
Tru1I TTAA 7 cut(s) 917, 1782, 1803, 1973, 2001, 2253, 2460
Tru9I TTAA 7 cut(s) 917, 1782, 1803, 1973, 2001, 2253, 2460
TseFI GTSAC 6 cut(s) 35, 220, 316, 847, 1009, 2529
TseI GCWGC 8 cut(s) 476, 569, 818, 886, 926, 1582, 1810, 2336
Tsp45I GTSAC 6 cut(s) 35, 220, 316, 847, 1009, 2529
TspGWI ACGGA 4 cut(s) 368, 413, 2148, 2667
Van91I CCANNNNNTGG 3 cut(s) 592, 1420, 2273
VpaK11BI GGWCC 2 cut(s) 751, 2575
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
XceI RCATGY 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 2556
XmnI GAANNNNTTC 1 cut(s) 1946
XspI CTAG 4 cut(s) 126, 711, 822, 2695
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.