Rroxscaffold_176G00431390

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0011365
Physical Location & Seq
Forward (+)
1032532 .. 1041392
8861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431390.1

Sequence Viewer

Length: 2988 bp
ATGGCTCAGCCTCTCGTGAAGAAAGACGACGATCGCGACGATGAAGGTAATGTCCGTTTTTCTGTTAGGCAAATCAATTTTCACCGCCCTCAAATTCGATCTGGTTTTTTCCGCTCCCAATCTTGCATACTGTTTCGGTGCTCTGAGTACTCTCCATTTCTGGGGATTGAAAAGGGGGCTGTTCTTCAAGAAGCTAGGGTCTTCAATGATCCTCAGCTAGACGCAAGAAGATGCTCTCAGGTTATCACAAAGCTTCTGTATCTACTGAATCAGGGTGAAACCTTCACTAAGGTTGAAGCTACAGAAGTTTTCTTTTCGGTGACCAAGCTTTTCCAGTCGAGAGATATAGGATTGAGGAGAATGGTTTATCTGATGATAAAAGAGTTATCGCCTTCTGCTGATGAGGTTATTATTGTGACAAGCTCCCTAATGAAGGACATGAATAGTAAAACTGATATGTATCGGGCCAATGCCATTCGTGTGCTTTGTCGGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATTGTTGACAAGAATCCAGTGGTTGCGAGTGCAGCTCTAGTTAGTGGGATCCATCTGCTCCAGACTAACCCAGAGATTGTTAAAAGGTGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGGGCAGCCCTTGTACAGTTTCATGCACTGGCTTTACTTCATCAGATAAGACAAAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGCACTGTCCGTTCTCCATTAGCTCAATGCCTGTTGATCCGCTATACTAGTCAGGTAATCCGGGAGTCTGCTGGGAGTACACAAGCAGGTGACCGTCCTTTTTATGATTATCTCGAGGGTTGCCTGCGGCACAAAGCAGAGATGGTAATTTTTGAAGCTGCCAGAGCAATTACAGAGCTCCATGGTGTGACCAACAGAGAATTGACTCCAGCTATCACCGTTCTTCAGCTATTTTTAAGTTCTTCAAAGCCAGTGTTAAGATTTGCTGCGGTCCGCACCCTAAACAAGGTGGCGATGACTCATCCAATGGCCGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCGATCACCACACTTCTGAAGACAGGAAATGAATCGAGTGTGGATCGCTTGATGAAGCAGATAACTAACTTCATGTCAGATATTGCTGATGAATTCAAAATTGTAGTTGTGGAAGCCATTAGATCTCTGTGTTTGAAGTTTCCATTGAAGTATAGAGCTCTCATGAACTTCCTAAGCAACATTCTCCGAGAAGAAGGCGGGTTTGAGTACAAAAAAGCAATTGTTGACTCAATTGTGATTCTCATTAGAGATATCCCAGAAGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATTGAAGATTGTGAATTTACCTATCTTTCTACTCAGATACTCCACTTTTTGGGGATTGAAGGGCCAAGAACCTCGGATCCAAGCAAATACATACGGTACATTTATAATCGAGTGCATCTTGAGAATGCCACCGTCCGGGCCAGTGCTGTGAGCACATTAGCCAAATTTGGTGCCATGGTTGATTCATTGAAGCCCCGAGTGTTTATTCTGTTAAGAAGGTGCCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTTAGTACACTTGGAGGGGATGGCTGTGTTGTTGAGACTGACCAGGATGTAAAGGACTTTCTCTTTGGGTCTCTGGATGTCCCCCTCGTTAATCTTGAGACAAGTTTGAAGAATTATGAGGCTTCAGAAGAACCATTTGACATCAATTCTGTACCCAAGGAGATCAAGTCTCAGCCACTTGCTGAGAAAAAGCCCAGAGTAAAAAGCCAACTGGTCTGGGTGCTCCACCAAGTGGCCCTGCATCCACAGTCGATGCTTATGAGAAGATGCTTTCTTCTATTCCAGAGTTTTCAAACTTTGGGAAACTTTTCAAGGTTTTGTGACATGAATATTCATGATGAATATTCATCTCTCCTTACTGCCATGCTTTCCTCAGCACCTGTGGAGTTGACAGAGGCAGAAACCGAATATGCAGTTAACGTTGTCAAGCACATTTTTGATAGGCATGTTGTGTTCCAGTACAACTGCACCAACACGATTCCCGAGCAATTACTGGAAAATGTTATTGTTGCCGTTGATGCTTCTGATGCAGAGGAGTTTACTGAAGTGGGATCCAAGCCTCTCCGGTCTCTTCCTTATGATACAGCTGGCCAGACCTTTTTGGCGTTTGAGAAGCCAGAAGGTGTCCTTGCAGTTGGAAAATTTTCAAACACATTGAGATTCATTGTTAAAGAGGTTGATCCAACCACTGGAGAGGCAGAGGAAGATGGTGTAGAAGATGAATACCAGCTGGAGGACCTTGAGGTGGTTGCTGCTGATTATATTTTGAAGGATCAAGTACTCAATTTCAGAAATGCATGGGAAACCATGGGCCCAGATTGTGAGCGTGTAGATGAATATGGCCTGGGCCAAAGAGAGAGCTTGAATGAAGCAGTCTCTGCTGTCATCAGCCTCCTTGGAATGCAGCCCTGTGAGGGCACGGAGGTTATTGCCAGCAACTCAAGGTCCCACACTTGCTTATTGGCTGGTGTATACATAGGCAATGTGAAGGTCCTCGTGCGGTTGTCTTTTGGAATTGATAGTTCAAAGGAGGTTGCGATGAAGCTTGTGGTTAGATCTGAGGATGTGACTGTCAGCGATGCCATCCATGAGATTCCGCTTTGTCTTTGGGTCCCAACGTCGCCGCAAGTCGCCGACCCTAAGAGGAATAATCCTCTCCGCAATGTGTCGCTTGTGCGCAGCCTTCCAACGTCAACTATGTTTAACGAACACCCAATCAGATCTGCAACATTCCGGGGTGCGAATGGTCTTGCACCTCTCAGAGAACAGCCCTTGCAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

995

Amino Acids

111.84

Weight (kDa)

5.61

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 59 - 572 2.1e-141 Adaptin N terminal region
Cnd1 PF12717 155 - 250 1.8e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 688 - 817 1.1e-45 Coatomer gamma subunit appendage platform subdomain
Coatomer_g_Cpla PF16381 820 - 932 3.9e-34 Coatomer subunit gamma-1 C-terminal appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1542
AarI CACCTGC 1 cut(s) 839
Acc16I TGCGCA 1 cut(s) 2882
Acc36I ACCTGC 1 cut(s) 839
AccB1I GGYRCC 2 cut(s) 1607, 1656
AccB7I CCANNNNNTGG 3 cut(s) 1486, 1936, 2393
AccBSI CCGCTC 2 cut(s) 114, 526
AccI GTMKAC 1 cut(s) 2676
AccII CGCG 1 cut(s) 36
AclI AACGTT 1 cut(s) 2124
AcoI YGGCCR 2 cut(s) 1071, 2293
AcsI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2345
AcuI CTGAAG 4 cut(s) 971, 1178, 1812, 2268
AdeI CACNNNGTG 1 cut(s) 1936
AfaI GTAC 9 cut(s) 149, 678, 841, 1349, 1535, 1711, 1857, 2165, 2484
AhlI ACTAGT 1 cut(s) 809
AjnI CCWGG 2 cut(s) 1746, 2547
AloI GAACNNNNNNTCC 2 cut(s) 1498, 1530
Alw21I GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1929
Alw26I GTCTC 6 cut(s) 1733, 1779, 1796, 1878, 2277, 2584
AlwNI CAGNNNCTG 2 cut(s) 2084, 2582
Ama87I CYCGRG 3 cut(s) 875, 1632, 2186
AoxI GGCC 9 cut(s) 465, 1071, 1499, 1575, 1938, 2293, 2515, 2545, 2551
ApaI GGGCCC 1 cut(s) 2519
ApeKI GCWGC 7 cut(s) 575, 668, 920, 1028, 2456, 2608, 2883
ApoI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2345
ArsI GACNNNNNNTTYG 4 cut(s) 709, 741, 1751, 1783
Asp700I GAANNNNTTC 2 cut(s) 2011, 2347
AspLEI GCGC 1 cut(s) 2883
AsuC2I CCSGG 3 cut(s) 824, 1574, 2939
AsuHPI GGTGA 6 cut(s) 74, 287, 331, 863, 970, 1138
AvaI CYCGRG 3 cut(s) 875, 1632, 2186
AvaII GGWCC 5 cut(s) 1033, 2440, 2649, 2695, 2815
BaeGI GKGCMC 3 cut(s) 767, 2519, 2624
BalI TGGCCA 1 cut(s) 2295
BamHI GGATCC 3 cut(s) 591, 1513, 2255
BanI GGYRCC 2 cut(s) 1607, 1656
BanII GRGCYC 3 cut(s) 942, 1300, 2519
BauI CACGAG 2 cut(s) 14, 2699
BbsI GAAGAC 2 cut(s) 193, 1166
Bbv12I GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1929
BbvCI CCTCAGC 2 cut(s) 213, 2077
BbvI GCAGC 7 cut(s) 587, 680, 907, 1015, 2443, 2620, 2895
BccI CCATC 6 cut(s) 494, 603, 898, 1718, 2405, 2795
BceAI ACGGC 2 cut(s) 1058, 2201
BciT130I CCWGG 2 cut(s) 1748, 2549
BcnI CCSGG 3 cut(s) 824, 1574, 2939
BcoDI GTCTC 6 cut(s) 1733, 1779, 1796, 1878, 2277, 2584
BcuI ACTAGT 1 cut(s) 809
BfaI CTAG 4 cut(s) 195, 218, 581, 810
BfmI CTRYAG 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 839
BglII AGATCT 3 cut(s) 1262, 2759, 2924
BisI GCNGC 9 cut(s) 576, 669, 890, 921, 1029, 2457, 2609, 2828, 2884
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 9 cut(s) 577, 670, 891, 922, 1030, 2458, 2610, 2829, 2885
BmcAI AGTACT 2 cut(s) 149, 2484
Bme1390I CCNGG 5 cut(s) 824, 1574, 1748, 2549, 2939
Bme18I GGWCC 5 cut(s) 1033, 2440, 2649, 2695, 2815
BmeT110I CYCGRG 3 cut(s) 875, 1632, 2186
BmrFI CCNGG 5 cut(s) 824, 1574, 1748, 2549, 2939
BmsI GCATC 8 cut(s) 221, 1561, 1947, 1954, 1961, 2212, 2221, 2773
BpiI GAAGAC 2 cut(s) 193, 1166
BplI GAGNNNNNCTC 2 cut(s) 562, 594
BpmI CTGGAG 4 cut(s) 587, 954, 2415, 2456
Bpu10I CCTNAGC 3 cut(s) 213, 1313, 2077
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 1577, 1820, 2465, 2629
BpuMI CCSGG 3 cut(s) 824, 1574, 2939
BsaBI GATNNNNATC 1 cut(s) 459
BsaI GGTCTC 2 cut(s) 1779, 2277
BsaJI CCNNGG 8 cut(s) 943, 1509, 1611, 1860, 2511, 2548, 2599, 2938
BsaWI WCCGGW 1 cut(s) 2268
BsaXI ACNNNNNCTCC 2 cut(s) 1856, 1886
Bse3DI GCAATG 3 cut(s) 1128, 2692, 2872
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 2 cut(s) 1748, 2549
BseDI CCNNGG 8 cut(s) 943, 1509, 1611, 1860, 2511, 2548, 2599, 2938
BseGI GGATG 8 cut(s) 505, 1063, 1729, 1756, 1786, 1945, 2773, 2787
BseJI GATNNNNATC 1 cut(s) 459
BseMI GCAATG 3 cut(s) 1128, 2692, 2872
BseRI GAGGAG 2 cut(s) 370, 2252
BseSI GKGCMC 3 cut(s) 767, 2519, 2624
BseXI GCAGC 7 cut(s) 587, 680, 907, 1015, 2443, 2620, 2895
BseYI CCCAGC 1 cut(s) 833
BsgI GTGCAG 2 cut(s) 594, 2155
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 9 cut(s) 467, 1073, 1501, 1577, 1940, 2295, 2517, 2547, 2553
BshNI GGYRCC 2 cut(s) 1607, 1656
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 5 cut(s) 143, 942, 1300, 1592, 1929
BsiHKCI CYCGRG 3 cut(s) 875, 1632, 2186
BsiSI CCGG 4 cut(s) 823, 1573, 2269, 2938
BslFI GGGAC 3 cut(s) 1769, 2635, 2801
BsmAI GTCTC 6 cut(s) 1733, 1779, 1796, 1878, 2277, 2584
BsmFI GGGAC 3 cut(s) 1769, 2635, 2801
BsmI GAATGC 2 cut(s) 1567, 2610
BsnI GGCC 9 cut(s) 467, 1073, 1501, 1577, 1940, 2295, 2517, 2547, 2553
Bso31I GGTCTC 2 cut(s) 1779, 2277
BsoBI CYCGRG 3 cut(s) 875, 1632, 2186
Bsp120I GGGCCC 1 cut(s) 2515
Bsp1286I GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1929, 2519, 2624
Bsp1407I TGTACA 1 cut(s) 676
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 3 cut(s) 943, 1611, 2511
Bsp68I TCGCGA 1 cut(s) 36
BspANI GGCC 9 cut(s) 467, 1073, 1501, 1577, 1940, 2295, 2517, 2547, 2553
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 2 cut(s) 1302, 2038
BspMI ACCTGC 1 cut(s) 839
BspT107I GGYRCC 2 cut(s) 1607, 1656
BspTNI GGTCTC 2 cut(s) 1779, 2277
BsrBI CCGCTC 2 cut(s) 114, 526
BsrDI GCAATG 3 cut(s) 1128, 2692, 2872
BsrGI TGTACA 1 cut(s) 676
BssECI CCNNGG 8 cut(s) 943, 1509, 1611, 1860, 2511, 2548, 2599, 2938
BssNAI GTATAC 1 cut(s) 2677
BssSI CACGAG 2 cut(s) 14, 2699
BssT1I CCWWGG 5 cut(s) 943, 1611, 1860, 2511, 2599
Bst1107I GTATAC 1 cut(s) 2677
Bst2BI CACGAG 2 cut(s) 14, 2699
Bst2UI CCWGG 2 cut(s) 1748, 2549
Bst6I CTCTTC 1 cut(s) 2280
BstAPI GCANNNNNTGC 1 cut(s) 2582
BstAUI TGTACA 1 cut(s) 676
BstC8I GCNNGC 4 cut(s) 887, 1418, 2293, 2638
BstDSI CCRYGG 3 cut(s) 943, 1611, 2511
BstEII GGTNACC 3 cut(s) 319, 745, 851
BstENI CCTNNNNNAGG 2 cut(s) 431, 1046
BstF5I GGATG 8 cut(s) 505, 1063, 1729, 1756, 1786, 1945, 2773, 2787
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 2883
BstMAI GTCTC 6 cut(s) 1733, 1779, 1796, 1878, 2277, 2584
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2222, 2231, 2582
BstNI CCWGG 2 cut(s) 1748, 2549
BstNSI RCATGY 1 cut(s) 2153
BstPI GGTNACC 3 cut(s) 319, 745, 851
BstSCI CCNGG 5 cut(s) 822, 1572, 1746, 2547, 2937
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 3 cut(s) 767, 2519, 2624
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 7 cut(s) 587, 680, 907, 1015, 2443, 2620, 2895
BstV2I GAAGAC 2 cut(s) 193, 1166
BstX2I RGATCY 6 cut(s) 591, 1262, 1513, 2255, 2759, 2924
BstYI RGATCY 6 cut(s) 591, 1262, 1513, 2255, 2759, 2924
BstZ17I GTATAC 1 cut(s) 2677
BsuRI GGCC 9 cut(s) 467, 1073, 1501, 1577, 1940, 2295, 2517, 2547, 2553
BtgI CCRYGG 3 cut(s) 943, 1611, 2511
BtgZI GCGATG 3 cut(s) 1070, 2756, 2796
BtsCI GGATG 8 cut(s) 505, 1063, 1729, 1756, 1786, 1945, 2773, 2787
BtsIMutI CAGTG 7 cut(s) 567, 689, 765, 1020, 1585, 1675, 2391
BtuMI TCGCGA 1 cut(s) 36
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 4 cut(s) 887, 1418, 2293, 2638
CaiI CAGNNNCTG 2 cut(s) 2084, 2582
CciI TCATGA 2 cut(s) 1302, 2038
CfoI GCGC 1 cut(s) 2883
CpoI CGGWCCG 1 cut(s) 1033
CseI GACGC 1 cut(s) 230
Csp6I GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1710, 1856, 2164, 2483
CspI CGGWCCG 1 cut(s) 1033
CviQI GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1710, 1856, 2164, 2483
DraIII CACNNNGTG 1 cut(s) 1936
EaeI YGGCCR 2 cut(s) 1071, 2293
Eam1104I CTCTTC 1 cut(s) 2280
EarI CTCTTC 1 cut(s) 2280
Ecl136II GAGCTC 2 cut(s) 940, 1298
Eco130I CCWWGG 5 cut(s) 943, 1611, 1860, 2511, 2599
Eco24I GRGCYC 3 cut(s) 942, 1300, 2519
Eco31I GGTCTC 2 cut(s) 1779, 2277
Eco32I GATATC 1 cut(s) 1393
Eco47I GGWCC 5 cut(s) 1033, 2440, 2649, 2695, 2815
Eco53kI GAGCTC 2 cut(s) 940, 1298
Eco57I CTGAAG 4 cut(s) 971, 1178, 1812, 2268
Eco88I CYCGRG 3 cut(s) 875, 1632, 2186
Eco91I GGTNACC 3 cut(s) 319, 745, 851
EcoICRI GAGCTC 2 cut(s) 940, 1298
EcoNI CCTNNNNNAGG 2 cut(s) 431, 1046
EcoO109I RGGNCCY 4 cut(s) 2440, 2649, 2695, 2815
EcoO65I GGTNACC 3 cut(s) 319, 745, 851
EcoRI GAATTC 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 1746, 2547
EcoRV GATATC 1 cut(s) 1393
EcoT14I CCWWGG 5 cut(s) 943, 1611, 1860, 2511, 2599
EcoT22I ATGCAT 1 cut(s) 2503
EcoT38I GRGCYC 3 cut(s) 942, 1300, 2519
ErhI CCWWGG 5 cut(s) 943, 1611, 1860, 2511, 2599
FalI AAGNNNNNCTT 2 cut(s) 2316, 2348
FaqI GGGAC 3 cut(s) 1769, 2635, 2801
FauI CCCGC 1 cut(s) 1331
FblI GTMKAC 1 cut(s) 2676
Fnu4HI GCNGC 9 cut(s) 576, 669, 890, 921, 1029, 2457, 2609, 2828, 2884
FokI GGATG 8 cut(s) 512, 1050, 1736, 1763, 1793, 1932, 2774, 2780
FriOI GRGCYC 3 cut(s) 942, 1300, 2519
Fsp4HI GCNGC 9 cut(s) 576, 669, 890, 921, 1029, 2457, 2609, 2828, 2884
FspBI CTAG 4 cut(s) 195, 218, 581, 810
FspI TGCGCA 1 cut(s) 2882
GlaI GCGC 1 cut(s) 2882
GluI GCNGC 9 cut(s) 576, 669, 890, 921, 1029, 2457, 2609, 2828, 2884
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 4 cut(s) 587, 954, 2415, 2456
HaeIII GGCC 9 cut(s) 467, 1073, 1501, 1577, 1940, 2295, 2517, 2547, 2553
HapII CCGG 4 cut(s) 823, 1573, 2269, 2938
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 2883
Hin6I GCGC 1 cut(s) 2881
HinP1I GCGC 1 cut(s) 2881
HincII GTYRAC 5 cut(s) 550, 1366, 2094, 2122, 2898
HindII GTYRAC 5 cut(s) 550, 1366, 2094, 2122, 2898
HindIII AAGCTT 3 cut(s) 251, 326, 2747
HpaI GTTAAC 1 cut(s) 2122
HpaII CCGG 4 cut(s) 823, 1573, 2269, 2938
HphI GGTGA 6 cut(s) 74, 287, 331, 863, 970, 1138
Hpy166II GTNNAC 9 cut(s) 550, 842, 1366, 1712, 2094, 2122, 2244, 2677, 2898
Hpy8I GTNNAC 9 cut(s) 550, 842, 1366, 1712, 2094, 2122, 2244, 2677, 2898
Hpy99I CGWCG 3 cut(s) 32, 41, 2827
HpyCH4IV ACGT 3 cut(s) 2124, 2822, 2894
HpyF10VI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2222, 2231, 2582
HpySE526I ACGT 3 cut(s) 2124, 2822, 2894
HspAI GCGC 1 cut(s) 2881
KflI GGGWCCC 1 cut(s) 2815
KspAI GTTAAC 1 cut(s) 2122
LmnI GCTCC 5 cut(s) 119, 428, 606, 945, 1932
Lsp1109I GCAGC 7 cut(s) 587, 680, 907, 1015, 2443, 2620, 2895
LweI GCATC 8 cut(s) 221, 1561, 1947, 1954, 1961, 2212, 2221, 2773
MaeI CTAG 4 cut(s) 195, 218, 581, 810
MaeII ACGT 3 cut(s) 2124, 2822, 2894
MaeIII GTNAC 8 cut(s) 319, 415, 745, 851, 949, 1075, 2024, 2770
MbiI CCGCTC 2 cut(s) 114, 526
MfeI CAATTG 2 cut(s) 1359, 1371
MflI RGATCY 6 cut(s) 591, 1262, 1513, 2255, 2759, 2924
MhlI GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1929, 2519, 2624
MlsI TGGCCA 1 cut(s) 2295
MluNI TGGCCA 1 cut(s) 2295
MlyI GAGTC 4 cut(s) 836, 961, 1054, 1361
MmeI TCCRAC 4 cut(s) 470, 2320, 2411, 2915
Mox20I TGGCCA 1 cut(s) 2295
Mph1103I ATGCAT 1 cut(s) 2503
MroXI GAANNNNTTC 2 cut(s) 2011, 2347
MscI TGGCCA 1 cut(s) 2295
MseI TTAA 8 cut(s) 624, 998, 1019, 1649, 1794, 2121, 2373, 2907
MslI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
Msp20I TGGCCA 1 cut(s) 2295
MspA1I CMGCKG 2 cut(s) 2291, 2434
MspI CCGG 4 cut(s) 823, 1573, 2269, 2938
MspR9I CCNGG 5 cut(s) 824, 1574, 1748, 2549, 2939
MunI CAATTG 2 cut(s) 1359, 1371
Mva1269I GAATGC 2 cut(s) 1567, 2610
MvaI CCWGG 2 cut(s) 1748, 2549
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 6 cut(s) 575, 926, 1139, 2222, 2231, 2582
NciI CCSGG 3 cut(s) 824, 1574, 2939
NcoI CCATGG 3 cut(s) 943, 1611, 2511
NmuCI GTSAC 7 cut(s) 319, 415, 851, 949, 1075, 2024, 2770
NruI TCGCGA 1 cut(s) 36
NsbI TGCGCA 1 cut(s) 2882
NsiI ATGCAT 1 cut(s) 2503
NspI RCATGY 1 cut(s) 2153
PaeR7I CTCGAG 1 cut(s) 875
PagI TCATGA 2 cut(s) 1302, 2038
PaqCI CACCTGC 1 cut(s) 839
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 2 cut(s) 1567, 2610
PdmI GAANNNNTTC 2 cut(s) 2011, 2347
PfeI GAWTC 8 cut(s) 268, 556, 1172, 1378, 1619, 2182, 2364, 2797
PflMI CCANNNNNTGG 3 cut(s) 1486, 1936, 2393
PfoI TCCNGGA 1 cut(s) 822
PkrI GCNGC 9 cut(s) 577, 670, 891, 922, 1030, 2458, 2610, 2829, 2885
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 4 cut(s) 835, 961, 1054, 1361
PpsI GAGTC 4 cut(s) 835, 961, 1054, 1361
PpuMI RGGWCCY 4 cut(s) 2440, 2649, 2695, 2815
PsiI TTATAA 1 cut(s) 1542
Psp124BI GAGCTC 2 cut(s) 942, 1300
Psp1406I AACGTT 1 cut(s) 2124
Psp5II RGGWCCY 4 cut(s) 2440, 2649, 2695, 2815
Psp6I CCWGG 2 cut(s) 1746, 2547
PspEI GGTNACC 3 cut(s) 319, 745, 851
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 2 cut(s) 1746, 2547
PspOMI GGGCCC 1 cut(s) 2515
PspPPI RGGWCCY 4 cut(s) 2440, 2649, 2695, 2815
PsrI GAACNNNNNNTAC 2 cut(s) 496, 528
PstNI CAGNNNCTG 2 cut(s) 2084, 2582
PsuI RGATCY 6 cut(s) 591, 1262, 1513, 2255, 2759, 2924
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 2 cut(s) 2291, 2434
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 9 cut(s) 149, 678, 841, 1349, 1535, 1711, 1857, 2165, 2484
RsaNI GTAC 9 cut(s) 148, 677, 840, 1348, 1534, 1710, 1856, 2164, 2483
RseI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
Rsr2I CGGWCCG 1 cut(s) 1033
RsrII CGGWCCG 1 cut(s) 1033
SacI GAGCTC 2 cut(s) 942, 1300
SaqAI TTAA 8 cut(s) 624, 998, 1019, 1649, 1794, 2121, 2373, 2907
SatI GCNGC 9 cut(s) 576, 669, 890, 921, 1029, 2457, 2609, 2828, 2884
ScaI AGTACT 2 cut(s) 149, 2484
SchI GAGTC 4 cut(s) 836, 961, 1054, 1361
ScrFI CCNGG 5 cut(s) 824, 1574, 1748, 2549, 2939
SduI GDGCHC 8 cut(s) 143, 767, 942, 1300, 1592, 1929, 2519, 2624
SfaNI GCATC 8 cut(s) 221, 1561, 1947, 1954, 1961, 2212, 2221, 2773
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 875
SinI GGWCC 5 cut(s) 1033, 2440, 2649, 2695, 2815
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 3 cut(s) 479, 1094, 1428
SmlI CTYRAG 5 cut(s) 875, 1556, 1799, 2444, 2644
SmoI CTYRAG 5 cut(s) 875, 1556, 1799, 2444, 2644
SpeI ACTAGT 1 cut(s) 809
SspI AATATT 2 cut(s) 2035, 2048
SspMI CTAG 4 cut(s) 195, 218, 581, 810
SstI GAGCTC 2 cut(s) 942, 1300
StyD4I CCNGG 5 cut(s) 822, 1572, 1746, 2547, 2937
StyI CCWWGG 5 cut(s) 943, 1611, 1860, 2511, 2599
TaiI ACGT 3 cut(s) 2127, 2825, 2897
TaqI TCGA 6 cut(s) 97, 338, 876, 1175, 1546, 1955
TatI WGTACW 7 cut(s) 147, 676, 839, 1347, 1709, 2163, 2482
TauI GCSGC 2 cut(s) 892, 2830
TfiI GAWTC 8 cut(s) 268, 556, 1172, 1378, 1619, 2182, 2364, 2797
Tru1I TTAA 8 cut(s) 624, 998, 1019, 1649, 1794, 2121, 2373, 2907
Tru9I TTAA 8 cut(s) 624, 998, 1019, 1649, 1794, 2121, 2373, 2907
TscAI CASTG 7 cut(s) 567, 696, 772, 1020, 1585, 1675, 2398
TseFI GTSAC 7 cut(s) 319, 415, 851, 949, 1075, 2024, 2770
TseI GCWGC 7 cut(s) 575, 668, 920, 1028, 2456, 2608, 2883
Tsp45I GTSAC 7 cut(s) 319, 415, 851, 949, 1075, 2024, 2770
TspGWI ACGGA 4 cut(s) 44, 512, 761, 2639
TspRI CASTG 7 cut(s) 567, 696, 772, 1020, 1585, 1675, 2398
Van91I CCANNNNNTGG 3 cut(s) 1486, 1936, 2393
VpaK11BI GGWCC 5 cut(s) 1033, 2440, 2649, 2695, 2815
XagI CCTNNNNNAGG 2 cut(s) 431, 1046
XapI RAATTY 5 cut(s) 93, 1232, 1451, 1601, 2345
XceI RCATGY 1 cut(s) 2153
XhoI CTCGAG 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 2676
XmnI GAANNNNTTC 2 cut(s) 2011, 2347
XspI CTAG 4 cut(s) 195, 218, 581, 810
ZrmI AGTACT 2 cut(s) 149, 2484
Zsp2I ATGCAT 1 cut(s) 2503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.