Rh6AG128800

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011365
Physical Location & Seq
Forward (+)
19418698 .. 19425733
7036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG128800.1

Sequence Viewer

Length: 2292 bp
ATGGCTCAGCCTCTCGTGAAGAAAGACGACGATCGCGACGATGAAGAGTACTCTCCATTTCTGGGGATTGAAAAGGGGGCTGTTCTTCAAGAAGCTAGGGTCTTTAATGATCCTCAGCTAGACGCAAGAAGATGCTCTCAGGTTATCACAAAGCTTCTGTATCTACTGAATCAGGGTGAAACCTTCACTAAGGTTGAAGCTACAGAAGTTTTCTTTTCGGTGACCAAGCTTTTCCAGTCAAGAGATATAGGATTGAGGAGAATGGTTTATCTGATGATAAAAGAGTTATCGCCTTCTGCTGATGAGGTTATTATTGTGACAAGCTCCCTAATGAAGGACATGAATAGTAAAACTGATATGTATCGGGCCAATGCCATTCGTGTGCTTTGTCGGATTACGGATGGAACCCTTCTTACCCAGATTGAGCGGTATTTGAAACAAGCCATTGTTGACAAGAATCCAGTGGTTGCGAGTGCAGCTCTAGTTAGTGGGATCCATCTACTCCAGACTAACCCAGAGATTGTTAAAAGGTGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGGGCAGCCCTTGTACAGTTTCATGCACTGGCTTTACTTCATCAGATAAGACAAAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGCACTGTCCGTTCTCCATTAGCTCAATGCCTGTTGATCCGCTATACTAGTCAGGTAATCCGGGAGTCCGCTGGGAGTACACAAGCAGGTGACCGTCCTTTTTATGATTATCTCGAGGGTTGCCTGCGGCACAAAGCAGAGATGGTAATTTTTGAAGCTGCCAGAGCAATTACAGAGCTCCATGGTGTGACCAACAGAGAATTGACTCCAGCTATCACCGTTCTTCAGCTATTTTTAAGTTCTTCAAAGCCAGTGTTAAGATTTGCTGCGGTCCGCACCCTAAACAAGGTGGCGATGACTCATCCAATGGCCGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCGATCACCACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGCTTGATGAAGCAGATAACTAACTTCATGTCAGATATTGCTGATGAATTCAAAATTGTAGTTGTGGAAGCCATTAGATCTCTGTGTTTGAAGTTTCCATTGAAGTATAGAGCTCTCATGAACTTCCTAAGCAACATTCTCCGAGAAGAAGGCGGGTTTGAGTACAAAAAAGCAATTGTTGACTCAATTGTGATTCTAATTAGAGATATCCCAGAGGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATTGAAGATTGTGAATTTACCTACCTTTCTACTCAGATACTCCACTTTTTGGGGATTGAAGGGCCAAGAACCTCGGATCCAAGCAAATACATACGGTACATTTATAATCGAGTGCATCTTGAGAATGCCACTGTCCGGGCCAGTGCTGTGAGCACATTAGCCAAATTTGGTGCCATGGTTGATTCATTGAAGCCCCGAGTGTTTATTCTGTTAAGAAGGTGCCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTTAGTACACTTGGAGGGGATGGTTGTGTTGTTGAGACTGACCAGGATGTAAAGGACTTTCTCTTTGGGTCTCTGGATGTCCCCCTCGTTAATCTTGAGACAAGTTTGAAGAATTATGAGGCTTCAGAAGAACCATTTGACGTCAATTCTGTACCCAAGGAGATTAAGTCTCAGCCACTTGCTGAGAAAAAAGCCCAGAGTAAAAAGCCAACTGGTCTGGGTGCTCCTCCAAGTGGCCCTGCATCCACAGTCGATGCTTATGAGAAGATGCTTTCTTCTATTCCAGAGTTTTCAAACTTTGGGAAACTTTTCAAGTCCTCAGCACCTGTGGAGTTGACAGAGGCAGAAACCGAATATGCAGTTAACGTTGTCAAGCACATTTTTGATAGGCATGTTGTGTTCCAGTACAACTGCACCAACACCATTCCCGAGCAATTACTGGAAAATGTTATTGTTGCTGTTGATGCTTCTGATGCAGAGGAGTTTACTGAAGTGGGATCCAAGCCTCTCAGGTCTCTTCCTTATGATACACCTGGCCAGACCTTTTTGGCGTTTGAGAAGCCAGAAGGTGTCCTTGCAGTTGGAAAATTTTCAAACACATTGAGATTCATTGTTAAAGAGGTACAAGTTCTTCTCTTCCTTGTTCTTAACAGGCTCTTAGGACCAGACTACTGGACATTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

763

Amino Acids

85.54

Weight (kDa)

5.71

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 539 1e-141 Adaptin N terminal region
Cnd1 PF12717 122 - 217 1.2e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 621 - 744 1.3e-42 Coatomer gamma subunit appendage platform subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
AarI CACCTGC 1 cut(s) 740
AatII GACGTC 1 cut(s) 1749
Acc36I ACCTGC 1 cut(s) 740
AccB1I GGYRCC 2 cut(s) 1508, 1557
AccB7I CCANNNNNTGG 1 cut(s) 1387
AccBSI CCGCTC 1 cut(s) 427
AccII CGCG 1 cut(s) 36
AciI CCGC 7 cut(s) 427, 703, 732, 790, 932, 937, 1239
AclI AACGTT 1 cut(s) 1971
AclWI GGATC 9 cut(s) 104, 487, 500, 694, 1092, 1409, 1422, 2097, 2110
AcoI YGGCCR 2 cut(s) 972, 2140
AcsI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
AcuI CTGAAG 4 cut(s) 872, 1079, 1713, 2115
AcyI GRCGYC 1 cut(s) 1746
AfaI GTAC 9 cut(s) 50, 579, 742, 1250, 1436, 1612, 1758, 2012, 2229
AfiI CCNNNNNNNGG 7 cut(s) 62, 334, 565, 949, 1387, 1473, 1838
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 2 cut(s) 1647, 2137
AloI GAACNNNNNNTCC 2 cut(s) 1399, 1431
Alw21I GWGCWC 4 cut(s) 843, 1201, 1493, 1831
Alw26I GTCTC 5 cut(s) 1634, 1680, 1697, 1779, 2124
AlwI GGATC 9 cut(s) 104, 487, 500, 694, 1092, 1409, 1422, 2097, 2110
AlwNI CAGNNNCTG 1 cut(s) 1931
Ama87I CYCGRG 3 cut(s) 776, 1533, 2033
AoxI GGCC 6 cut(s) 366, 972, 1400, 1476, 1840, 2140
ApeKI GCWGC 4 cut(s) 476, 569, 821, 929
ApoI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
ArsI GACNNNNNNTTYG 4 cut(s) 610, 642, 1652, 1684
Asp700I GAANNNNTTC 2 cut(s) 1913, 2194
AspS9I GGNCC 6 cut(s) 366, 934, 1400, 1476, 1841, 2267
AsuC2I CCSGG 2 cut(s) 725, 1475
AsuHPI GGTGA 5 cut(s) 188, 232, 764, 871, 1039
AvaI CYCGRG 3 cut(s) 776, 1533, 2033
AvaII GGWCC 2 cut(s) 934, 2267
BaeGI GKGCMC 1 cut(s) 668
BalI TGGCCA 1 cut(s) 2142
BamHI GGATCC 3 cut(s) 492, 1414, 2102
BanI GGYRCC 2 cut(s) 1508, 1557
BanII GRGCYC 2 cut(s) 843, 1201
BarI GAAGNNNNNNTAC 2 cut(s) 2220, 2252
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 1067
Bbv12I GWGCWC 4 cut(s) 843, 1201, 1493, 1831
BbvCI CCTCAGC 2 cut(s) 114, 1924
BbvI GCAGC 4 cut(s) 488, 581, 808, 916
BccI CCATC 4 cut(s) 395, 504, 799, 1619
BceAI ACGGC 1 cut(s) 959
BciT130I CCWGG 2 cut(s) 1649, 2139
BcnI CCSGG 2 cut(s) 725, 1475
BcoDI GTCTC 5 cut(s) 1634, 1680, 1697, 1779, 2124
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 96, 119, 482, 711
BfmI CTRYAG 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 740
BglII AGATCT 1 cut(s) 1163
BisI GCNGC 5 cut(s) 477, 570, 791, 822, 930
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 5 cut(s) 478, 571, 792, 823, 931
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 725, 1475, 1649, 2139
Bme18I GGWCC 2 cut(s) 934, 2267
BmeT110I CYCGRG 3 cut(s) 776, 1533, 2033
BmgT120I GGNCC 6 cut(s) 366, 934, 1400, 1476, 1841, 2267
BmiI GGNNCC 6 cut(s) 406, 494, 1416, 1510, 1559, 2104
BmrFI CCNGG 4 cut(s) 725, 1475, 1649, 2139
BmsI GCATC 7 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068
BpiI GAAGAC 1 cut(s) 1067
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 2 cut(s) 488, 855
Bpu10I CCTNAGC 3 cut(s) 114, 1214, 1924
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 1478, 1721
BpuMI CCSGG 2 cut(s) 725, 1475
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 1746
BsaI GGTCTC 2 cut(s) 1680, 2124
BsaJI CCNNGG 4 cut(s) 844, 1410, 1512, 1761
BsaXI ACNNNNNCTCC 2 cut(s) 1757, 1787
Bsc4I CCNNNNNNNGG 7 cut(s) 62, 334, 565, 949, 1387, 1473, 1838
Bse3DI GCAATG 1 cut(s) 1029
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 2 cut(s) 1649, 2139
BseDI CCNNGG 4 cut(s) 844, 1410, 1512, 1761
BseGI GGATG 6 cut(s) 406, 964, 1630, 1657, 1687, 1847
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 7 cut(s) 62, 334, 565, 949, 1387, 1473, 1838
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 8 cut(s) 20, 128, 152, 1385, 1779, 1790, 1938, 2128
BseRI GAGGAG 3 cut(s) 271, 1821, 2099
BseSI GKGCMC 1 cut(s) 668
BseXI GCAGC 4 cut(s) 488, 581, 808, 916
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 2 cut(s) 495, 2002
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 6 cut(s) 368, 974, 1402, 1478, 1842, 2142
BshNI GGYRCC 2 cut(s) 1508, 1557
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 4 cut(s) 843, 1201, 1493, 1831
BsiHKCI CYCGRG 3 cut(s) 776, 1533, 2033
BsiSI CCGG 2 cut(s) 724, 1474
BslFI GGGAC 1 cut(s) 1670
BslI CCNNNNNNNGG 7 cut(s) 62, 334, 565, 949, 1387, 1473, 1838
BsmAI GTCTC 5 cut(s) 1634, 1680, 1697, 1779, 2124
BsmFI GGGAC 1 cut(s) 1670
BsmI GAATGC 1 cut(s) 1468
BsnI GGCC 6 cut(s) 368, 974, 1402, 1478, 1842, 2142
Bso31I GGTCTC 2 cut(s) 1680, 2124
BsoBI CYCGRG 3 cut(s) 776, 1533, 2033
Bsp1286I GDGCHC 5 cut(s) 668, 843, 1201, 1493, 1831
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 844, 1512
Bsp68I TCGCGA 1 cut(s) 36
BspACI CCGC 7 cut(s) 427, 703, 732, 790, 932, 937, 1239
BspANI GGCC 6 cut(s) 368, 974, 1402, 1478, 1842, 2142
BspCNI CTCAG 8 cut(s) 19, 127, 151, 1384, 1780, 1789, 1937, 2127
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 6 cut(s) 406, 494, 1416, 1510, 1559, 2104
BspMI ACCTGC 1 cut(s) 740
BspPI GGATC 9 cut(s) 104, 487, 500, 694, 1092, 1409, 1422, 2097, 2110
BspT107I GGYRCC 2 cut(s) 1508, 1557
BspTNI GGTCTC 2 cut(s) 1680, 2124
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 1029
BsrGI TGTACA 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 844, 1410, 1512, 1761
BssMI GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
BssNI GRCGYC 1 cut(s) 1746
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 844, 1512, 1761
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 2 cut(s) 1649, 2139
Bst4CI ACNGT 9 cut(s) 582, 626, 670, 758, 883, 1434, 1471, 1571, 1855
Bst6I CTCTTC 3 cut(s) 39, 2127, 2246
BstACI GRCGYC 1 cut(s) 1746
BstAUI TGTACA 1 cut(s) 577
BstC8I GCNNGC 2 cut(s) 788, 1319
BstDSI CCRYGG 2 cut(s) 844, 1512
BstEII GGTNACC 3 cut(s) 220, 646, 752
BstENI CCTNNNNNAGG 2 cut(s) 332, 947
BstF5I GGATG 6 cut(s) 406, 964, 1630, 1657, 1687, 1847
BstFNI CGCG 1 cut(s) 36
BstKTI GATC 9 cut(s) 34, 112, 495, 702, 1047, 1087, 1166, 1417, 2105
BstMAI GTCTC 5 cut(s) 1634, 1680, 1697, 1779, 2124
BstMBI GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 476, 827, 1040, 2069, 2078
BstNI CCWGG 2 cut(s) 1649, 2139
BstNSI RCATGY 1 cut(s) 2000
BstPI GGTNACC 3 cut(s) 220, 646, 752
BstSCI CCNGG 4 cut(s) 723, 1473, 1647, 2137
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 668
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 4 cut(s) 488, 581, 808, 916
BstV2I GAAGAC 1 cut(s) 1067
BstX2I RGATCY 4 cut(s) 492, 1163, 1414, 2102
BstXI CCANNNNNNTGG 1 cut(s) 2277
BstYI RGATCY 4 cut(s) 492, 1163, 1414, 2102
BsuRI GGCC 6 cut(s) 368, 974, 1402, 1478, 1842, 2142
BtgI CCRYGG 2 cut(s) 844, 1512
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 6 cut(s) 406, 964, 1630, 1657, 1687, 1847
BtsIMutI CAGTG 7 cut(s) 468, 590, 666, 921, 1467, 1486, 1576
BtuMI TCGCGA 1 cut(s) 36
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 2 cut(s) 788, 1319
CaiI CAGNNNCTG 1 cut(s) 1931
CciI TCATGA 1 cut(s) 1203
Cfr13I GGNCC 6 cut(s) 366, 934, 1400, 1476, 1841, 2267
CpoI CGGWCCG 1 cut(s) 934
CseI GACGC 1 cut(s) 131
Csp6I GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2228
CspI CGGWCCG 1 cut(s) 934
CviAII CATG 7 cut(s) 340, 587, 845, 1114, 1204, 1513, 1997
CviQI GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2228
DpnI GATC 9 cut(s) 33, 111, 494, 701, 1046, 1086, 1165, 1416, 2104
DpnII GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
EaeI YGGCCR 2 cut(s) 972, 2140
Eam1104I CTCTTC 3 cut(s) 39, 2127, 2246
EarI CTCTTC 3 cut(s) 39, 2127, 2246
Ecl136II GAGCTC 2 cut(s) 841, 1199
Eco130I CCWWGG 3 cut(s) 844, 1512, 1761
Eco24I GRGCYC 2 cut(s) 843, 1201
Eco31I GGTCTC 2 cut(s) 1680, 2124
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 2 cut(s) 934, 2267
Eco53kI GAGCTC 2 cut(s) 841, 1199
Eco57I CTGAAG 4 cut(s) 872, 1079, 1713, 2115
Eco88I CYCGRG 3 cut(s) 776, 1533, 2033
Eco91I GGTNACC 3 cut(s) 220, 646, 752
EcoICRI GAGCTC 2 cut(s) 841, 1199
EcoNI CCTNNNNNAGG 2 cut(s) 332, 947
EcoO65I GGTNACC 3 cut(s) 220, 646, 752
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 2 cut(s) 1647, 2137
EcoRV GATATC 1 cut(s) 1294
EcoT14I CCWWGG 3 cut(s) 844, 1512, 1761
EcoT38I GRGCYC 2 cut(s) 843, 1201
ErhI CCWWGG 3 cut(s) 844, 1512, 1761
FaeI CATG 7 cut(s) 343, 590, 848, 1117, 1207, 1516, 2000
FalI AAGNNNNNCTT 2 cut(s) 2163, 2195
FaqI GGGAC 1 cut(s) 1670
FatI CATG 7 cut(s) 339, 586, 844, 1113, 1203, 1512, 1996
FauI CCCGC 1 cut(s) 1232
Fnu4HI GCNGC 5 cut(s) 477, 570, 791, 822, 930
FokI GGATG 6 cut(s) 413, 951, 1637, 1664, 1694, 1834
FriOI GRGCYC 2 cut(s) 843, 1201
Fsp4HI GCNGC 5 cut(s) 477, 570, 791, 822, 930
FspBI CTAG 4 cut(s) 96, 119, 482, 711
GluI GCNGC 5 cut(s) 477, 570, 791, 822, 930
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 2 cut(s) 488, 855
HaeIII GGCC 6 cut(s) 368, 974, 1402, 1478, 1842, 2142
HapII CCGG 2 cut(s) 724, 1474
HgaI GACGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 1746
Hin1II CATG 7 cut(s) 343, 590, 848, 1117, 1207, 1516, 2000
HincII GTYRAC 4 cut(s) 451, 1267, 1941, 1969
HindII GTYRAC 4 cut(s) 451, 1267, 1941, 1969
HindIII AAGCTT 2 cut(s) 152, 227
HpaI GTTAAC 1 cut(s) 1969
HpaII CCGG 2 cut(s) 724, 1474
HphI GGTGA 5 cut(s) 188, 232, 764, 871, 1039
Hpy166II GTNNAC 7 cut(s) 451, 743, 1267, 1613, 1941, 1969, 2091
Hpy8I GTNNAC 7 cut(s) 451, 743, 1267, 1613, 1941, 1969, 2091
Hpy99I CGWCG 2 cut(s) 32, 41
HpyAV CCTTC 8 cut(s) 193, 303, 328, 419, 1229, 1391, 1548, 2165
HpyCH4III ACNGT 9 cut(s) 582, 626, 670, 758, 883, 1434, 1471, 1571, 1855
HpyCH4IV ACGT 2 cut(s) 1746, 1971
HpyCH4V TGCA 9 cut(s) 476, 590, 987, 1453, 1847, 1964, 2019, 2081, 2183
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 827, 1040, 2069, 2078
HpySE526I ACGT 2 cut(s) 1746, 1971
Hsp92I GRCGYC 1 cut(s) 1746
Hsp92II CATG 7 cut(s) 343, 590, 848, 1117, 1207, 1516, 2000
KspAI GTTAAC 1 cut(s) 1969
Kzo9I GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
LmnI GCTCC 3 cut(s) 329, 846, 1834
Lsp1109I GCAGC 4 cut(s) 488, 581, 808, 916
LweI GCATC 7 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068
MaeI CTAG 4 cut(s) 96, 119, 482, 711
MaeII ACGT 2 cut(s) 1746, 1971
MaeIII GTNAC 6 cut(s) 220, 316, 646, 752, 850, 976
MalI GATC 9 cut(s) 33, 111, 494, 701, 1046, 1086, 1165, 1416, 2104
MbiI CCGCTC 1 cut(s) 427
MboI GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
MfeI CAATTG 2 cut(s) 1260, 1272
MflI RGATCY 4 cut(s) 492, 1163, 1414, 2102
MhlI GDGCHC 5 cut(s) 668, 843, 1201, 1493, 1831
MlsI TGGCCA 1 cut(s) 2142
MluNI TGGCCA 1 cut(s) 2142
MlyI GAGTC 4 cut(s) 737, 862, 955, 1262
MmeI TCCRAC 2 cut(s) 371, 2167
Mox20I TGGCCA 1 cut(s) 2142
MroXI GAANNNNTTC 2 cut(s) 1913, 2194
MscI TGGCCA 1 cut(s) 2142
MslI CAYNNNNRTG 3 cut(s) 380, 995, 1329
Msp20I TGGCCA 1 cut(s) 2142
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 2 cut(s) 724, 1474
MspR9I CCNGG 4 cut(s) 725, 1475, 1649, 2139
MunI CAATTG 2 cut(s) 1260, 1272
Mva1269I GAATGC 1 cut(s) 1468
MvaI CCWGG 2 cut(s) 1649, 2139
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 5 cut(s) 476, 827, 1040, 2069, 2078
NciI CCSGG 2 cut(s) 725, 1475
NcoI CCATGG 2 cut(s) 844, 1512
NdeII GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
NlaIII CATG 7 cut(s) 343, 590, 848, 1117, 1207, 1516, 2000
NlaIV GGNNCC 6 cut(s) 406, 494, 1416, 1510, 1559, 2104
NmuCI GTSAC 5 cut(s) 220, 316, 752, 850, 976
NruI TCGCGA 1 cut(s) 36
NspI RCATGY 1 cut(s) 2000
PaeR7I CTCGAG 1 cut(s) 776
PagI TCATGA 1 cut(s) 1203
PaqCI CACCTGC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 1 cut(s) 1468
PdmI GAANNNNTTC 2 cut(s) 1913, 2194
PfeI GAWTC 6 cut(s) 169, 457, 1073, 1279, 1520, 2211
PflMI CCANNNNNTGG 1 cut(s) 1387
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 5 cut(s) 478, 571, 792, 823, 931
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 4 cut(s) 736, 862, 955, 1262
PpsI GAGTC 4 cut(s) 736, 862, 955, 1262
PsiI TTATAA 1 cut(s) 1443
Psp124BI GAGCTC 2 cut(s) 843, 1201
Psp1406I AACGTT 1 cut(s) 1971
Psp6I CCWGG 2 cut(s) 1647, 2137
PspEI GGTNACC 3 cut(s) 220, 646, 752
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 2 cut(s) 1647, 2137
PspN4I GGNNCC 6 cut(s) 406, 494, 1416, 1510, 1559, 2104
PspPI GGNCC 6 cut(s) 366, 934, 1400, 1476, 1841, 2267
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstNI CAGNNNCTG 1 cut(s) 1931
PsuI RGATCY 4 cut(s) 492, 1163, 1414, 2102
PvuI CGATCG 1 cut(s) 34
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 9 cut(s) 50, 579, 742, 1250, 1436, 1612, 1758, 2012, 2229
RsaNI GTAC 9 cut(s) 49, 578, 741, 1249, 1435, 1611, 1757, 2011, 2228
RseI CAYNNNNRTG 3 cut(s) 380, 995, 1329
Rsr2I CGGWCCG 1 cut(s) 934
RsrII CGGWCCG 1 cut(s) 934
SacI GAGCTC 2 cut(s) 843, 1201
SatI GCNGC 5 cut(s) 477, 570, 791, 822, 930
Sau3AI GATC 9 cut(s) 31, 109, 492, 699, 1044, 1084, 1163, 1414, 2102
Sau96I GGNCC 6 cut(s) 366, 934, 1400, 1476, 1841, 2267
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 4 cut(s) 737, 862, 955, 1262
ScrFI CCNGG 4 cut(s) 725, 1475, 1649, 2139
SduI GDGCHC 5 cut(s) 668, 843, 1201, 1493, 1831
SfaNI GCATC 7 cut(s) 122, 1462, 1849, 1856, 1863, 2059, 2068
SfcI CTRYAG 1 cut(s) 201
Sfr274I CTCGAG 1 cut(s) 776
SinI GGWCC 2 cut(s) 934, 2267
SlaI CTCGAG 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 380, 995, 1329
SmlI CTYRAG 3 cut(s) 776, 1457, 1700
SmoI CTYRAG 3 cut(s) 776, 1457, 1700
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 7 cut(s) 427, 703, 732, 790, 932, 937, 1239
SspMI CTAG 4 cut(s) 96, 119, 482, 711
SstI GAGCTC 2 cut(s) 843, 1201
StyD4I CCNGG 4 cut(s) 723, 1473, 1647, 2137
StyI CCWWGG 3 cut(s) 844, 1512, 1761
TaaI ACNGT 9 cut(s) 582, 626, 670, 758, 883, 1434, 1471, 1571, 1855
TaiI ACGT 2 cut(s) 1749, 1974
TaqI TCGA 3 cut(s) 777, 1447, 1857
TatI WGTACW 6 cut(s) 48, 577, 740, 1248, 1610, 2010
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 6 cut(s) 169, 457, 1073, 1279, 1520, 2211
TscAI CASTG 7 cut(s) 468, 597, 673, 921, 1474, 1486, 1576
TseFI GTSAC 5 cut(s) 220, 316, 752, 850, 976
TseI GCWGC 4 cut(s) 476, 569, 821, 929
Tsp45I GTSAC 5 cut(s) 220, 316, 752, 850, 976
TspGWI ACGGA 2 cut(s) 413, 662
TspRI CASTG 7 cut(s) 468, 597, 673, 921, 1474, 1486, 1576
Van91I CCANNNNNTGG 1 cut(s) 1387
VpaK11BI GGWCC 2 cut(s) 934, 2267
XagI CCTNNNNNAGG 2 cut(s) 332, 947
XapI RAATTY 4 cut(s) 1133, 1352, 1502, 2192
XceI RCATGY 1 cut(s) 2000
XhoI CTCGAG 1 cut(s) 776
XmnI GAANNNNTTC 2 cut(s) 1913, 2194
XspI CTAG 4 cut(s) 96, 119, 482, 711
ZraI GACGTC 1 cut(s) 1747
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.