MD10G1077400.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011365
Physical Location & Seq
Forward (+)
10945038 .. 10950137
5100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1077400.v1.1.491

Sequence Viewer

Length: 2292 bp
ATGGCTCAGCCTCTCGTGAAGAAGGACGACGATCGCGACGACGAAGAGTACTCTCCGTTTCTGGGGATTGAGAAGGGTGCTGTTCTTCAGGAAGCGAGGGTTTTTAATGATCCTCAACTCGACGCTAGAAGATGCTCCCAGGTTATCACAAAGCTTTTGTATCTACTGAACCAGGGGGAAACCTTTACAAAGGTTGAAGCTACAGAAGTTTTCTTTTCCGTGACCAAGCTTTTCCAATCGAGAGATATAGGATTGAGGAGAATGGTTTATCTGATCATAAAAGAGTTGTCCCCCTCTGCAGATGAGGTTATTATTGTGACAAGCTCCCTGATGAAGGATATGAATAGTAAAACAGACATGTATCGGGCAAATGCCATCCGTGTCCTCTGTCGGATTACAGATGGAACCCTTCTTACCCAGATTGAGCGGTATCTGAAACAAGCCATTGTTGATAAGAATCCAGTGGTTGCAAGTGCAGCTCTAGTCAGTGGGATCCATCTGCTCCAGACTAACCCAGAGATTGTCAAAAGATGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGAGCAGCCCTTGTACAGTTTCATGCACTGGCTTTACTTCATCAGATACGACAGAATGACCGTCTTGCTGTGAGCAAGTTGGTTACCAGCTTGACAAGGGGGACTGTCCGCTCTCCATTGGCCCAGTGCCTTTTGATTCGTTATACTAGTCAGGTTATCCGCGAGTCTGGAAATGCACAAGCGGGCGACCGTCCCTTTTACGATTATCTTGAGGCTTGTCTTCGCCACAAAGCGGAGATGGTGATTTTGGAGGCTGCTAGAGCAATTACAGAGCTCCATGGTGTGACCCCCAGAGAACTTACTCCAGCAATCACTGTTCTGCAGCTATTTCTAAGCTCTTCGAAACCAGTTCTAAGATTTGCTGCTGTCCGCACCTTGAACAAGGCATCCTTCTTCTCCTTTTACTCTACTAAACATTGTGTGGCAATGACACATCCAGTGCCTGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGCATTGCCACACTTGCAATCACAACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGCTTGATGAAGCAGATAACTAATTTCATGTCAGATATCGCTGACGAGTTCAAAATTGTTGTTGTGGAAGCCATAAGATCGTTGTGTTTGAAATTTCCTTTGAAGTTCAGAGCTCTGATGAACTTTTTGAGCAACATTCTCAGAGAAGAAGGTGGATTTGAGTACAAAAAGGCAATTGTTGATTCAATTGTGATTCTCATTAGAGATATCCCTGATGCAAAAGAAAGTGGCTTGCTTCATCTCTGTGAGTTCATCGAAGATTGTGAATTCACCTATCTTTCTACACAGATACTCCACTTTTTGGGCATTGAAGGGCCAAAAACCTCTGATCCAAGCAAGTATATAAGGTACATATATAATCGAGTACATCTTGAGAATGCCACTGTTCGCGCGAGTGCAGTTAGTACATTGGCAAAATTTGGTGCTATGGTTGATTCATTGAAGCCGCGAGTGTTTATTCTGCTGCGACGATGTCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTAAATACGCTTGGAGGAGATGGTTCAGTTGTTGAGACTGACAATGATGTAAAGGACTTCCTCTTTGGGTCTCTGGATGTCCCACTTGTCAATCTGGAGACAAGTTTGAAGAATTATGAGGCTTCGGAAGAACCATTTGACATTAATTCTGTACCCAAGGAGGTTAAGTCGCAGCCACTTGCTGAGAAGAAAGCTCAAAGTAAAAAGCCAACTGGTCTTGGTGCTCTTCCAACTGGCCCTGCATCCACAGTTGCTGCTTATGAGACATTGCTTTCCTCTATTCCGGAGTATTCAAACTTTGGGAAACTATTCAAGTCCTCAGCACCTGTGGAGTTAACAGAGCCAGAAACTGAATATGCAGTTAATGTTGTCAAGCACATTTTTGATAGCCATGTTGTGTTCCAGTACAACTGCACCAATACCATTCCCGAGCAATTACTGGAAGATGTCATTGTCGTGGTTGATGCTTCAGAAGCTGAGGAATTTTCTGAAGTGGCATCCAGGTCTCTCAGGTCTCTTCCATATGATACACCTGGACAAACCTTTTTGGCATTTGAGAAGCCAGAAGGTGTCCCTGCAGTTGGAAAATTTACAAACTTGTTGAGGTTCATTGTTAAAGAGGTATCAAGTTCTTCCCTTTATCCCTGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

764

Amino Acids

85.27

Weight (kDa)

5.74

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 26 - 550 2e-135 Adaptin N terminal region
Cnd1 PF12717 122 - 217 1.3e-06 non-SMC mitotic condensation complex subunit 1
COP-gamma_platf PF08752 632 - 758 6.2e-40 Coatomer gamma subunit appendage platform subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1420
AccBSI CCGCTC 2 cut(s) 427, 675
AccII CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
AccIII TCCGGA 1 cut(s) 1921
AciI CCGC 7 cut(s) 427, 673, 724, 746, 797, 934, 1565
AclWI GGATC 5 cut(s) 104, 487, 500, 1125, 1442
AcsI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
AcuI CTGAAG 4 cut(s) 71, 1112, 2091, 2148
AfaI GTAC 8 cut(s) 50, 579, 1283, 1469, 1485, 1525, 1791, 2045
AfiI CCNNNNNNNGG 5 cut(s) 62, 334, 796, 1420, 2219
AflIII ACRYGT 1 cut(s) 357
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 4 cut(s) 138, 171, 2138, 2170
AloI GAACNNNNNNTCC 2 cut(s) 935, 967
Alw21I GWGCWC 3 cut(s) 840, 1234, 1864
Alw26I GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
AlwI GGATC 5 cut(s) 104, 487, 500, 1125, 1442
AlwNI CAGNNNCTG 5 cut(s) 1007, 1892, 1964, 1988, 2114
Ama87I CYCGRG 1 cut(s) 2066
Aor13HI TCCGGA 1 cut(s) 1921
AoxI GGCC 3 cut(s) 684, 1433, 1873
ApeKI GCWGC 8 cut(s) 476, 569, 818, 886, 926, 1582, 1810, 1892
ApoI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
ArsI GACNNNNNNTTYG 2 cut(s) 1685, 1717
AseI ATTAAT 1 cut(s) 1782
Asp700I GAANNNNTTC 1 cut(s) 1946
AspLEI GCGC 1 cut(s) 1511
AspS9I GGNCC 3 cut(s) 685, 1433, 1874
AsuHPI GGTGA 2 cut(s) 817, 1381
AsuII TTCGAA 1 cut(s) 905
AvaI CYCGRG 1 cut(s) 2066
BamHI GGATCC 1 cut(s) 492
BanII GRGCYC 2 cut(s) 840, 1234
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 776, 1100
Bbv12I GWGCWC 3 cut(s) 840, 1234, 1864
BbvCI CCTCAGC 2 cut(s) 1957, 2115
BbvI GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 1879
BccI CCATC 6 cut(s) 383, 395, 504, 525, 796, 1652
BciT130I CCWGG 4 cut(s) 140, 173, 2140, 2172
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 126, 482, 711, 822
BfmI CTRYAG 4 cut(s) 201, 297, 884, 2214
BisI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1565, 1583, 1811, 1893
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1566, 1584, 1812, 1894
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 140, 173, 2140, 2172
BmeT110I CYCGRG 1 cut(s) 2066
BmgT120I GGNCC 3 cut(s) 685, 1433, 1874
BmiI GGNNCC 2 cut(s) 406, 494
BmrFI CCNGG 4 cut(s) 140, 173, 2140, 2172
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 6 cut(s) 122, 959, 1324, 1889, 2092, 2144
BmuI ACTGGG 1 cut(s) 682
BpiI GAAGAC 2 cut(s) 776, 1100
BpmI CTGGAG 3 cut(s) 488, 852, 1754
Bpu10I CCTNAGC 2 cut(s) 1957, 2115
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 905
BpuEI CTTGAG 2 cut(s) 794, 1511
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 3 cut(s) 1713, 2148, 2157
BsaJI CCNNGG 4 cut(s) 138, 172, 841, 1794
BsaWI WCCGGW 1 cut(s) 1921
BsaXI ACNNNNNCTCC 4 cut(s) 1395, 1425, 1790, 1820
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 334, 796, 1420, 2219
Bse3DI GCAATG 3 cut(s) 996, 1062, 1904
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 1921
BseBI CCWGG 4 cut(s) 140, 173, 2140, 2172
BseDI CCNNGG 4 cut(s) 138, 172, 841, 1794
BseGI GGATG 6 cut(s) 375, 950, 997, 1720, 1880, 2135
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 5 cut(s) 62, 334, 796, 1420, 2219
BseMI GCAATG 3 cut(s) 996, 1062, 1904
BseMII CTCAG 6 cut(s) 20, 1273, 1812, 1971, 2106, 2161
BseRI GAGGAG 2 cut(s) 271, 1668
BseXI GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 1879
BsgI GTGCAG 3 cut(s) 495, 1536, 2035
Bsh1236I CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
Bsh1285I CGRYCG 2 cut(s) 34, 754
BshFI GGCC 3 cut(s) 686, 1435, 1875
BsiEI CGRYCG 2 cut(s) 34, 754
BsiHKAI GWGCWC 3 cut(s) 840, 1234, 1864
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 1 cut(s) 1922
BslFI GGGAC 5 cut(s) 274, 679, 741, 1703, 2195
BslI CCNNNNNNNGG 5 cut(s) 62, 334, 796, 1420, 2219
BsmAI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BsmFI GGGAC 5 cut(s) 274, 679, 741, 1703, 2195
BsmI GAATGC 1 cut(s) 1501
BsnI GGCC 3 cut(s) 686, 1435, 1875
Bso31I GGTCTC 3 cut(s) 1713, 2148, 2157
BsoBI CYCGRG 1 cut(s) 2066
Bsp119I TTCGAA 1 cut(s) 905
Bsp1286I GDGCHC 3 cut(s) 840, 1234, 1864
Bsp13I TCCGGA 1 cut(s) 1921
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 841
Bsp68I TCGCGA 1 cut(s) 36
BspACI CCGC 7 cut(s) 427, 673, 724, 746, 797, 934, 1565
BspANI GGCC 3 cut(s) 686, 1435, 1875
BspCNI CTCAG 6 cut(s) 19, 1272, 1813, 1970, 2107, 2160
BspEI TCCGGA 1 cut(s) 1921
BspFNI CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
BspLI GGNNCC 2 cut(s) 406, 494
BspMAI CTGCAG 3 cut(s) 301, 888, 2218
BspPI GGATC 5 cut(s) 104, 487, 500, 1125, 1442
BspQI GCTCTTC 2 cut(s) 907, 1869
BspT104I TTCGAA 1 cut(s) 905
BspTNI GGTCTC 3 cut(s) 1713, 2148, 2157
BsrBI CCGCTC 2 cut(s) 427, 675
BsrDI GCAATG 3 cut(s) 996, 1062, 1904
BsrGI TGTACA 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 138, 172, 841, 1794
BssMI GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 2 cut(s) 841, 1794
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 4 cut(s) 140, 173, 2140, 2172
Bst4CI ACNGT 8 cut(s) 582, 626, 670, 755, 880, 1504, 1604, 1888
Bst6I CTCTTC 4 cut(s) 39, 907, 1869, 2160
BstAUI TGTACA 1 cut(s) 577
BstBI TTCGAA 1 cut(s) 905
BstC8I GCNNGC 2 cut(s) 748, 1352
BstDEI CTNAG 8 cut(s) 6, 896, 917, 1259, 1821, 1957, 2115, 2147
BstDSI CCRYGG 1 cut(s) 841
BstEII GGTNACC 1 cut(s) 646
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 6 cut(s) 375, 950, 997, 1720, 1880, 2135
BstFNI CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
BstHHI GCGC 1 cut(s) 1511
BstKTI GATC 7 cut(s) 34, 112, 276, 495, 1120, 1199, 1450
BstMAI GTCTC 7 cut(s) 1598, 1667, 1713, 1730, 1895, 2148, 2157
BstMBI GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
BstMCI CGRYCG 2 cut(s) 34, 754
BstMWI GCNNNNNNNGC 4 cut(s) 476, 824, 1073, 2111
BstNI CCWGG 4 cut(s) 140, 173, 2140, 2172
BstNSI RCATGY 1 cut(s) 361
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 4 cut(s) 138, 171, 2138, 2170
BstSFI CTRYAG 4 cut(s) 201, 297, 884, 2214
BstUI CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
BstV1I GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 1879
BstV2I GAAGAC 2 cut(s) 776, 1100
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BsuRI GGCC 3 cut(s) 686, 1435, 1875
BtgI CCRYGG 1 cut(s) 841
BtsCI GGATG 6 cut(s) 375, 950, 997, 1720, 1880, 2135
BtsIMutI CAGTG 8 cut(s) 468, 493, 590, 695, 876, 1008, 1500, 1609
BtuMI TCGCGA 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 748, 1352
CaiI CAGNNNCTG 5 cut(s) 1007, 1892, 1964, 1988, 2114
CfoI GCGC 1 cut(s) 1511
Cfr13I GGNCC 3 cut(s) 685, 1433, 1874
CseI GACGC 1 cut(s) 131
Csp6I GTAC 8 cut(s) 49, 578, 1282, 1468, 1484, 1524, 1790, 2044
CviAII CATG 5 cut(s) 358, 587, 842, 1147, 2030
CviQI GTAC 8 cut(s) 49, 578, 1282, 1468, 1484, 1524, 1790, 2044
DdeI CTNAG 8 cut(s) 6, 896, 917, 1259, 1821, 1957, 2115, 2147
DpnI GATC 7 cut(s) 33, 111, 275, 494, 1119, 1198, 1449
DpnII GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
Eam1104I CTCTTC 4 cut(s) 39, 907, 1869, 2160
EarI CTCTTC 4 cut(s) 39, 907, 1869, 2160
Ecl136II GAGCTC 2 cut(s) 838, 1232
Eco130I CCWWGG 2 cut(s) 841, 1794
Eco24I GRGCYC 2 cut(s) 840, 1234
Eco31I GGTCTC 3 cut(s) 1713, 2148, 2157
Eco32I GATATC 2 cut(s) 1156, 1327
Eco53kI GAGCTC 2 cut(s) 838, 1232
Eco57I CTGAAG 4 cut(s) 71, 1112, 2091, 2148
Eco88I CYCGRG 1 cut(s) 2066
Eco91I GGTNACC 1 cut(s) 646
EcoICRI GAGCTC 2 cut(s) 838, 1232
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 4 cut(s) 138, 171, 2138, 2170
EcoRV GATATC 2 cut(s) 1156, 1327
EcoT14I CCWWGG 2 cut(s) 841, 1794
EcoT38I GRGCYC 2 cut(s) 840, 1234
ErhI CCWWGG 2 cut(s) 841, 1794
FaeI CATG 5 cut(s) 361, 590, 845, 1150, 2033
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 5 cut(s) 274, 679, 741, 1703, 2195
FatI CATG 5 cut(s) 357, 586, 841, 1146, 2029
FauI CCCGC 1 cut(s) 739
FauNDI CATATG 1 cut(s) 2161
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1565, 1583, 1811, 1893
FokI GGATG 6 cut(s) 362, 937, 984, 1727, 1867, 2122
FriOI GRGCYC 2 cut(s) 840, 1234
Fsp4HI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1565, 1583, 1811, 1893
FspBI CTAG 4 cut(s) 126, 482, 711, 822
GlaI GCGC 1 cut(s) 1510
GluI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1565, 1583, 1811, 1893
GsuI CTGGAG 3 cut(s) 488, 852, 1754
HaeIII GGCC 3 cut(s) 686, 1435, 1875
HapII CCGG 1 cut(s) 1922
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 1511
Hin1II CATG 5 cut(s) 361, 590, 845, 1150, 2033
Hin6I GCGC 1 cut(s) 1509
HinP1I GCGC 1 cut(s) 1509
HincII GTYRAC 1 cut(s) 1974
HindII GTYRAC 1 cut(s) 1974
HindIII AAGCTT 2 cut(s) 152, 227
HinfI GANTC 7 cut(s) 457, 700, 728, 1106, 1301, 1312, 1553
HpaI GTTAAC 1 cut(s) 1974
HpaII CCGG 1 cut(s) 1922
HphI GGTGA 2 cut(s) 817, 1381
Hpy166II GTNNAC 1 cut(s) 1974
Hpy8I GTNNAC 1 cut(s) 1974
Hpy99I CGWCG 5 cut(s) 32, 41, 44, 125, 1590
HpyAV CCTTC 8 cut(s) 16, 67, 328, 419, 964, 1262, 1424, 2198
HpyCH4III ACNGT 8 cut(s) 582, 626, 670, 755, 880, 1504, 1604, 1888
HpyF10VI GCNNNNNNNGC 4 cut(s) 476, 824, 1073, 2111
HpyF3I CTNAG 8 cut(s) 6, 896, 917, 1259, 1821, 1957, 2115, 2147
Hsp92II CATG 5 cut(s) 361, 590, 845, 1150, 2033
HspAI GCGC 1 cut(s) 1509
Kpn2I TCCGGA 1 cut(s) 1921
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 1974
Kzo9I GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
LguI GCTCTTC 2 cut(s) 907, 1869
LmnI GCTCC 4 cut(s) 140, 329, 507, 843
Lsp1109I GCAGC 8 cut(s) 488, 581, 805, 898, 913, 1569, 1822, 1879
LweI GCATC 6 cut(s) 122, 959, 1324, 1889, 2092, 2144
MaeI CTAG 4 cut(s) 126, 482, 711, 822
MaeIII GTNAC 5 cut(s) 220, 316, 646, 847, 1009
MalI GATC 7 cut(s) 33, 111, 275, 494, 1119, 1198, 1449
MbiI CCGCTC 2 cut(s) 427, 675
MboI GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
MfeI CAATTG 2 cut(s) 1293, 1305
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 3 cut(s) 840, 1234, 1864
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 3 cut(s) 371, 1892, 2200
MroI TCCGGA 1 cut(s) 1921
MroXI GAANNNNTTC 1 cut(s) 1946
MseI TTAA 6 cut(s) 105, 1782, 1803, 1973, 2001, 2253
MslI CAYNNNNRTG 3 cut(s) 1028, 1362, 2093
MspI CCGG 1 cut(s) 1922
MspR9I CCNGG 4 cut(s) 140, 173, 2140, 2172
MunI CAATTG 2 cut(s) 1293, 1305
Mva1269I GAATGC 1 cut(s) 1501
MvaI CCWGG 4 cut(s) 140, 173, 2140, 2172
MvnI CGCG 5 cut(s) 36, 726, 1509, 1511, 1567
MwoI GCNNNNNNNGC 4 cut(s) 476, 824, 1073, 2111
NcoI CCATGG 1 cut(s) 841
NdeI CATATG 1 cut(s) 2161
NdeII GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
NlaIII CATG 5 cut(s) 361, 590, 845, 1150, 2033
NlaIV GGNNCC 2 cut(s) 406, 494
NmuCI GTSAC 4 cut(s) 220, 316, 847, 1009
NruI TCGCGA 1 cut(s) 36
NspI RCATGY 1 cut(s) 361
NspV TTCGAA 1 cut(s) 905
PciI ACATGT 1 cut(s) 357
PciSI GCTCTTC 2 cut(s) 907, 1869
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 36
PctI GAATGC 1 cut(s) 1501
PdmI GAANNNNTTC 1 cut(s) 1946
PfeI GAWTC 6 cut(s) 457, 700, 1106, 1301, 1312, 1553
PflFI GACNNNGTC 1 cut(s) 1590
PflMI CCANNNNNTGG 1 cut(s) 1420
PkrI GCNGC 9 cut(s) 478, 571, 820, 888, 928, 1566, 1584, 1812, 1894
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PscI ACATGT 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 1782
Psp124BI GAGCTC 2 cut(s) 840, 1234
Psp6I CCWGG 4 cut(s) 138, 171, 2138, 2170
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 4 cut(s) 138, 171, 2138, 2170
PspN4I GGNNCC 2 cut(s) 406, 494
PspPI GGNCC 3 cut(s) 685, 1433, 1874
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstI CTGCAG 3 cut(s) 301, 888, 2218
PstNI CAGNNNCTG 5 cut(s) 1007, 1892, 1964, 1988, 2114
PsuI RGATCY 1 cut(s) 492
PsyI GACNNNGTC 1 cut(s) 1590
PvuI CGATCG 1 cut(s) 34
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 8 cut(s) 50, 579, 1283, 1469, 1485, 1525, 1791, 2045
RsaNI GTAC 8 cut(s) 49, 578, 1282, 1468, 1484, 1524, 1790, 2044
RseI CAYNNNNRTG 3 cut(s) 1028, 1362, 2093
SacI GAGCTC 2 cut(s) 840, 1234
SapI GCTCTTC 2 cut(s) 907, 1869
SaqAI TTAA 6 cut(s) 105, 1782, 1803, 1973, 2001, 2253
SatI GCNGC 9 cut(s) 477, 570, 819, 887, 927, 1565, 1583, 1811, 1893
Sau3AI GATC 7 cut(s) 31, 109, 273, 492, 1117, 1196, 1447
Sau96I GGNCC 3 cut(s) 685, 1433, 1874
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 4 cut(s) 140, 173, 2140, 2172
SduI GDGCHC 3 cut(s) 840, 1234, 1864
SfaNI GCATC 6 cut(s) 122, 959, 1324, 1889, 2092, 2144
SfcI CTRYAG 4 cut(s) 201, 297, 884, 2214
SfuI TTCGAA 1 cut(s) 905
SmiMI CAYNNNNRTG 3 cut(s) 1028, 1362, 2093
SmlI CTYRAG 2 cut(s) 773, 1490
SmoI CTYRAG 2 cut(s) 773, 1490
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 7 cut(s) 427, 673, 724, 746, 797, 934, 1565
SspMI CTAG 4 cut(s) 126, 482, 711, 822
SstI GAGCTC 2 cut(s) 840, 1234
StyD4I CCNGG 4 cut(s) 138, 171, 2138, 2170
StyI CCWWGG 2 cut(s) 841, 1794
TaaI ACNGT 8 cut(s) 582, 626, 670, 755, 880, 1504, 1604, 1888
TaqI TCGA 5 cut(s) 120, 239, 905, 1374, 1480
TatI WGTACW 6 cut(s) 48, 577, 1281, 1483, 1523, 2043
TauI GCSGC 1 cut(s) 1567
TfiI GAWTC 6 cut(s) 457, 700, 1106, 1301, 1312, 1553
Tru1I TTAA 6 cut(s) 105, 1782, 1803, 1973, 2001, 2253
Tru9I TTAA 6 cut(s) 105, 1782, 1803, 1973, 2001, 2253
TscAI CASTG 8 cut(s) 468, 493, 597, 695, 883, 1008, 1507, 1609
TseFI GTSAC 4 cut(s) 220, 316, 847, 1009
TseI GCWGC 8 cut(s) 476, 569, 818, 886, 926, 1582, 1810, 1892
Tsp45I GTSAC 4 cut(s) 220, 316, 847, 1009
TspGWI ACGGA 3 cut(s) 45, 208, 368
TspRI CASTG 8 cut(s) 468, 493, 597, 695, 883, 1008, 1507, 1609
Tth111I GACNNNGTC 1 cut(s) 1590
Van91I CCANNNNNTGG 1 cut(s) 1420
VspI ATTAAT 1 cut(s) 1782
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 5 cut(s) 1211, 1385, 1535, 2120, 2225
XceI RCATGY 1 cut(s) 361
XmnI GAANNNNTTC 1 cut(s) 1946
XspI CTAG 4 cut(s) 126, 482, 711, 822
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.