Prupe.8G098200_v2.0.a1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0011365
Physical Location & Seq
Forward (+)
12989838 .. 12996465
6628 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G098200.1

Sequence Viewer

Length: 2661 bp
ATGGCTCAGCCTCTCGTAAAGAAGGACGACGATCGTGACGATGAAGAGTACTCTCCGTTTCTGGGGATCGAGAAGGGTGCTGTTCTTCAGGAAGCCAGGGTTTTCAATGATCCTCAGCTAGACTCTAGAAGATGCTCTCAGGTCATTACGAAGCTTCTGTATCTACTGAACCAGGGAGAAACCTTTACAAAGGTTGAAGCTACGGAAGTTTTCTTTTCCGTGACTAAGCTTTTCCAATCGAGAGATATAGGATTGAGGAGAATGGTTTATCTGATCATAAAAGAGCTCTCTCCCTCTGCGGATGAGGTTATTATTGTGACAAGCTCCCTGATGAAGGATATGAATAGTAAAACTGATATGTATCGGGCAAATGCCATCCGTGTGCTGTGTCGGATTACAGATGGAACCCTTCTGACCCAGATTGAGCGGTATTTAAAACAAGCCATTGTCGACAAGAATCCAGTGGTTGCAAGTGCAGCTCTAGTTAGTGGGATCCATCTACTCCAGACTAACCCAGAGATTGTCAAAAGGTGGAGTAATGAGGTTCAGGAAGCTGTCCAGTCAAGAGCTGCCCTCGTACAGTTCCATGCACTGGCTTTGCTCCATCAGATACGACAGAATGACCGTCTTGCTGTTAGCAAGTTGGTTACCAGCTTGACAAGGGGGACTGTCCGTTCACCATTGGCCCAATGTCTATTGATCCGATATACTAGTCAGGTTATCCGCGAGTCAGCTGGAAATGCACAAATGGGCGACCGTCCTTTTTACGATTATCTTGAGGGGTGTCTTCGCCACAAAGCAGAGATGGTGATTTTTGAGGCTGCCAGAGCAATTACAGAGCTCCATGGCGTGACCACCAGAGAACTGACTCCTGCAATCACAGTTCTTCAGCTATTTTTGAGCTCTTCCAAGCCAGTGCTAAGATTTGCTGCTGTCCGCACTTTGAACAAGGTGGCAATGACACATCCAATGGCTGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGAAGTATTGCCACACTGGCAATCACCACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGCTTGATGAAGCAAATAACTAATTTCATGTCAGATATTGCTGATGAGTTCAAAATTGTAGTTGTGGAAGCCATAAGATCATTGTGTTTGAAGTTTCCACTGAAGTACAGAGCTCTGATGAACTTTCTCAGCAACATTCTCAGAGAAGAAGGTGGATTTGAGTACAAAAAGGCAATTGTTGATTCAATTGTGATTCTCATAAGAGATATCCCTGATGCAAAGGAAAGTGGCTTGCTTCATCTTTGTGAGTTCATTGAAGATTGTGAATTCACCTATCTTTCTACACAGATACTCCACTTTTTGGGTATTGAAGGGCCAAAAACCTCTGATCCAAGCAAATACATACGGTATATTTATAACCGAGTGCATCTTGAGAATGCCACTGTTCGAGCCAGTGCTGTGAGCACATTGGCAAAATTTGGTGCTTTGGTTGATTCATTGAAGCCACGAGTATTTATTCTGTTAAGACGATGTCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACGCTCTATCTAAATACGCTTGGAGGAGATGGTTCAGTAGTTGAGACTGACAGTGATGTAAAGGACTTCCTCTTTGGGTCTCTGGATGTGCCACTTGTCAATCTGGAGACAAGTTTGAAGAATTATGAGGCTTCAGAAGAACCTTTTGACATTAATTCTGTACCCAAGGAGATTAAGTCCCAGCCACTTGCTGAGAAGAAAGCCCAGAGTAAAAAGCCAACTGGTCTGGGTGTGACTCCAAGTGCCCCTGTATCCACAGTTGATGCTTATGAGAAGTTGCTTTCCTCCATTCCGGAGTTTTCAAACTTTGGCAAGCTATTCAAGTCCTCAGCACCTGTGGAGTTGACAGAGCCAGAAACCGAATATGCAGTTAATGTTGTCAAGCACATTTTTGATAGTCATGTTGTATTCCAGTACAACTGCACCAATACCATTCCCGAGCAATTACTGGAAAATGTTATTGTGGCCGTGGATGCTTCAGAAGCAGAGGAATTTTCTGAAGTTGCATCTAAGCCTCTTGCGTCTCTTCCTTATGATACCCCTGGACAGACCTTTTTGGCATTTGAGAGGCCAGAAGGTGTCCCCGCAGTCGGAAAATTTTCGAACACATTGAGGTTCATTGTTAAAGAGGTTGATCCAACCACTGGCGAGGCAGAGGAAGATGGTGTGGAAGATGAATACCAGCTGGAGGATCTTGAGGTTGTACCTGCTGATTACATTTTGAAGGTTCCAGTCTTCAATTTCAGGAATGCATGGGAAAGCATGGGACCTGATTTTGAGCGGATAGATGAATATGGCCTGGGACAAAGGGAGAGCTTGACTGAAGCTGTTAATACTGTCATCAACCTCCTTGGACTGCAGCCCTGTGAGGGCACAGAGGTTCTCGCCAGCAACTCAAGGTCACACACATGTCTATTGTCTGGTGTATACATAGGCAATGTGAAGGTCCTTGTGCGGTTGTCTTTTGGAATAGATAGTTCAAGGGAGGTTGCCATGAAACTGGCCGTTAGATCTGAGGATGAAGCCGTCAGTGATGCCATTCATGAGATTGTGGGCAGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

887

Amino Acids

98.67

Weight (kDa)

5.07

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
Acc36I ACCTGC 1 cut(s) 2311
AccB7I CCANNNNNTGG 3 cut(s) 592, 1387, 2240
AccBSI CCGCTC 2 cut(s) 427, 2377
AccI GTMKAC 2 cut(s) 450, 2523
AccII CGCG 1 cut(s) 726
AccIII TCCGGA 1 cut(s) 1888
AciI CCGC 8 cut(s) 299, 427, 724, 937, 2181, 2377, 2551, 2655
AclWI GGATC 9 cut(s) 74, 104, 487, 500, 694, 1092, 1409, 2225, 2295
AcoI YGGCCR 2 cut(s) 2061, 2598
AcsI RAATTY 4 cut(s) 1352, 1502, 2087, 2192
AcuI CTGAAG 8 cut(s) 71, 872, 1079, 1208, 1713, 2058, 2115, 2439
AfaI GTAC 7 cut(s) 50, 579, 1193, 1250, 1758, 2012, 2301
AfiI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1387, 2186, 2240, 2284, 2465
AflIII ACRYGT 1 cut(s) 2504
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 4 cut(s) 95, 171, 2137, 2394
AloI GAACNNNNNNTCC 2 cut(s) 658, 690
Alw21I GWGCWC 5 cut(s) 288, 843, 905, 1201, 1493
Alw26I GTCTC 5 cut(s) 1565, 1634, 1680, 1697, 2124
AlwI GGATC 9 cut(s) 74, 104, 487, 500, 694, 1092, 1409, 2225, 2295
AlwNI CAGNNNCTG 1 cut(s) 1931
Ama87I CYCGRG 1 cut(s) 2033
Aor13HI TCCGGA 1 cut(s) 1888
AoxI GGCC 6 cut(s) 684, 1400, 2061, 2165, 2392, 2598
ApeKI GCWGC 6 cut(s) 476, 569, 821, 929, 2455, 2652
ApoI RAATTY 4 cut(s) 1352, 1502, 2087, 2192
ArsI GACNNNNNNTTYG 2 cut(s) 1652, 1684
AseI ATTAAT 1 cut(s) 1749
Asp700I GAANNNNTTC 1 cut(s) 2194
AspS9I GGNCC 4 cut(s) 685, 1400, 2363, 2542
AsuHPI GGTGA 4 cut(s) 669, 820, 1039, 1348
AsuII TTCGAA 1 cut(s) 2198
AvaI CYCGRG 1 cut(s) 2033
AvaII GGWCC 2 cut(s) 2363, 2542
BaeGI GKGCMC 2 cut(s) 1843, 2471
BamHI GGATCC 1 cut(s) 492
BanII GRGCYC 4 cut(s) 288, 843, 905, 1201
BauI CACGAG 1 cut(s) 1533
BbsI GAAGAC 3 cut(s) 779, 1067, 2323
Bbv12I GWGCWC 5 cut(s) 288, 843, 905, 1201, 1493
BbvCI CCTCAGC 2 cut(s) 114, 1924
BbvI GCAGC 5 cut(s) 488, 556, 808, 916, 2467
BccI CCATC 7 cut(s) 383, 395, 504, 612, 799, 1619, 2252
BceAI ACGGC 3 cut(s) 2048, 2585, 2606
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 4 cut(s) 97, 173, 2139, 2396
BciVI GTATCC 1 cut(s) 1858
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 5 cut(s) 1565, 1634, 1680, 1697, 2124
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 5 cut(s) 119, 126, 482, 711, 2659
BfmI CTRYAG 1 cut(s) 2453
BfuAI ACCTGC 1 cut(s) 2311
BfuI GTATCC 1 cut(s) 1858
BglI GCCNNNNNGGC 1 cut(s) 1040
BglII AGATCT 1 cut(s) 2606
BisI GCNGC 7 cut(s) 477, 570, 822, 930, 2456, 2653, 2656
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 7 cut(s) 478, 571, 823, 931, 2457, 2654, 2657
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 97, 173, 2139, 2396
Bme18I GGWCC 2 cut(s) 2363, 2542
BmeT110I CYCGRG 1 cut(s) 2033
BmgT120I GGNCC 4 cut(s) 685, 1400, 2363, 2542
BmiI GGNNCC 4 cut(s) 406, 494, 2325, 2364
BmrFI CCNGG 4 cut(s) 97, 173, 2139, 2396
BmsI GCATC 7 cut(s) 122, 1291, 1462, 1849, 2059, 2111, 2620
BpiI GAAGAC 3 cut(s) 779, 1067, 2323
BplI GAGNNNNNCTC 2 cut(s) 558, 590
BpmI CTGGAG 3 cut(s) 488, 1721, 2303
Bpu10I CCTNAGC 2 cut(s) 114, 1924
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 2198
BpuEI CTTGAG 4 cut(s) 797, 1478, 2312, 2476
BsaBI GATNNNNATC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 1680
BsaJI CCNNGG 8 cut(s) 96, 172, 844, 1761, 2064, 2137, 2395, 2446
BsaWI WCCGGW 1 cut(s) 1888
BsaXI ACNNNNNCTCC 4 cut(s) 1362, 1392, 1757, 1787
Bsc4I CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1387, 2186, 2240, 2284, 2465
Bse3DI GCAATG 2 cut(s) 963, 2539
Bse8I GATNNNNATC 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 1888
BseBI CCWGG 4 cut(s) 97, 173, 2139, 2396
BseDI CCNNGG 8 cut(s) 96, 172, 844, 1761, 2064, 2137, 2395, 2446
BseGI GGATG 6 cut(s) 307, 375, 964, 1687, 2074, 2620
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1387, 2186, 2240, 2284, 2465
BseMI GCAATG 2 cut(s) 963, 2539
BseMII CTCAG 8 cut(s) 20, 128, 152, 1228, 1240, 1779, 1938, 2601
BseRI GAGGAG 2 cut(s) 271, 1635
BseSI GKGCMC 2 cut(s) 1843, 2471
BseXI GCAGC 5 cut(s) 488, 556, 808, 916, 2467
BseYI CCCAGC 1 cut(s) 1776
BsgI GTGCAG 2 cut(s) 495, 2002
Bsh1236I CGCG 1 cut(s) 726
Bsh1285I CGRYCG 2 cut(s) 34, 757
BshFI GGCC 6 cut(s) 686, 1402, 2063, 2167, 2394, 2600
BsiEI CGRYCG 2 cut(s) 34, 757
BsiHKAI GWGCWC 5 cut(s) 288, 843, 905, 1201, 1493
BsiHKCI CYCGRG 1 cut(s) 2033
BsiSI CCGG 1 cut(s) 1889
BslFI GGGAC 5 cut(s) 679, 1759, 2162, 2376, 2412
BslI CCNNNNNNNGG 8 cut(s) 62, 334, 592, 1387, 2186, 2240, 2284, 2465
BsmAI GTCTC 5 cut(s) 1565, 1634, 1680, 1697, 2124
BsmBI CGTCTC 1 cut(s) 2124
BsmFI GGGAC 5 cut(s) 679, 1759, 2162, 2376, 2412
BsmI GAATGC 2 cut(s) 1468, 2350
BsnI GGCC 6 cut(s) 686, 1402, 2063, 2167, 2394, 2600
Bso31I GGTCTC 1 cut(s) 1680
BsoBI CYCGRG 1 cut(s) 2033
Bsp119I TTCGAA 1 cut(s) 2198
Bsp1286I GDGCHC 7 cut(s) 288, 843, 905, 1201, 1493, 1843, 2471
Bsp13I TCCGGA 1 cut(s) 1888
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 844
BspACI CCGC 8 cut(s) 299, 427, 724, 937, 2181, 2377, 2551, 2655
BspANI GGCC 6 cut(s) 686, 1402, 2063, 2167, 2394, 2600
BspCNI CTCAG 8 cut(s) 19, 127, 151, 1227, 1239, 1780, 1937, 2602
BspEI TCCGGA 1 cut(s) 1888
BspFNI CGCG 1 cut(s) 726
BspHI TCATGA 1 cut(s) 2638
BspLI GGNNCC 4 cut(s) 406, 494, 2325, 2364
BspMAI CTGCAG 1 cut(s) 2457
BspMI ACCTGC 1 cut(s) 2311
BspPI GGATC 9 cut(s) 74, 104, 487, 500, 694, 1092, 1409, 2225, 2295
BspQI GCTCTTC 1 cut(s) 910
BspT104I TTCGAA 1 cut(s) 2198
BspTNI GGTCTC 1 cut(s) 1680
BsrBI CCGCTC 2 cut(s) 427, 2377
BsrDI GCAATG 2 cut(s) 963, 2539
BssECI CCNNGG 8 cut(s) 96, 172, 844, 1761, 2064, 2137, 2395, 2446
BssNAI GTATAC 1 cut(s) 2524
BssSI CACGAG 1 cut(s) 1533
BssT1I CCWWGG 3 cut(s) 844, 1761, 2446
Bst1107I GTATAC 1 cut(s) 2524
Bst2BI CACGAG 1 cut(s) 1533
Bst2UI CCWGG 4 cut(s) 97, 173, 2139, 2396
Bst6I CTCTTC 3 cut(s) 39, 910, 2127
BstBI TTCGAA 1 cut(s) 2198
BstC8I GCNNGC 3 cut(s) 1319, 1910, 2485
BstDSI CCRYGG 2 cut(s) 844, 2064
BstEII GGTNACC 1 cut(s) 646
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 6 cut(s) 307, 375, 964, 1687, 2074, 2620
BstFNI CGCG 1 cut(s) 726
BstMAI GTCTC 5 cut(s) 1565, 1634, 1680, 1697, 2124
BstMCI CGRYCG 2 cut(s) 34, 757
BstMWI GCNNNNNNNGC 6 cut(s) 476, 740, 827, 1040, 2069, 2078
BstNI CCWGG 4 cut(s) 97, 173, 2139, 2396
BstNSI RCATGY 1 cut(s) 2508
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 4 cut(s) 95, 171, 2137, 2394
BstSFI CTRYAG 1 cut(s) 2453
BstSLI GKGCMC 2 cut(s) 1843, 2471
BstUI CGCG 1 cut(s) 726
BstV1I GCAGC 5 cut(s) 488, 556, 808, 916, 2467
BstV2I GAAGAC 3 cut(s) 779, 1067, 2323
BstX2I RGATCY 3 cut(s) 492, 2287, 2606
BstXI CCANNNNNNTGG 1 cut(s) 2596
BstYI RGATCY 3 cut(s) 492, 2287, 2606
BstZ17I GTATAC 1 cut(s) 2524
BsuI GTATCC 1 cut(s) 1858
BsuRI GGCC 6 cut(s) 686, 1402, 2063, 2167, 2394, 2600
BtgI CCRYGG 2 cut(s) 844, 2064
BtsCI GGATG 6 cut(s) 307, 375, 964, 1687, 2074, 2620
BveI ACCTGC 1 cut(s) 2311
Cac8I GCNNGC 3 cut(s) 1319, 1910, 2485
CaiI CAGNNNCTG 1 cut(s) 1931
CciI TCATGA 1 cut(s) 2638
Cfr13I GGNCC 4 cut(s) 685, 1400, 2363, 2542
CseI GACGC 1 cut(s) 2106
Csp6I GTAC 7 cut(s) 49, 578, 1192, 1249, 1757, 2011, 2300
CviAII CATG 9 cut(s) 587, 845, 1114, 1997, 2349, 2359, 2505, 2590, 2639
CviQI GTAC 7 cut(s) 49, 578, 1192, 1249, 1757, 2011, 2300
DraI TTTAAA 1 cut(s) 435
EaeI YGGCCR 2 cut(s) 2061, 2598
Eam1104I CTCTTC 3 cut(s) 39, 910, 2127
EarI CTCTTC 3 cut(s) 39, 910, 2127
Ecl136II GAGCTC 4 cut(s) 286, 841, 903, 1199
Eco130I CCWWGG 3 cut(s) 844, 1761, 2446
Eco24I GRGCYC 4 cut(s) 288, 843, 905, 1201
Eco31I GGTCTC 1 cut(s) 1680
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 2 cut(s) 2363, 2542
Eco53kI GAGCTC 4 cut(s) 286, 841, 903, 1199
Eco57I CTGAAG 8 cut(s) 71, 872, 1079, 1208, 1713, 2058, 2115, 2439
Eco88I CYCGRG 1 cut(s) 2033
Eco91I GGTNACC 1 cut(s) 646
EcoICRI GAGCTC 4 cut(s) 286, 841, 903, 1199
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 2363, 2542
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 1352
EcoRII CCWGG 4 cut(s) 95, 171, 2137, 2394
EcoRV GATATC 1 cut(s) 1294
EcoT14I CCWWGG 3 cut(s) 844, 1761, 2446
EcoT22I ATGCAT 1 cut(s) 2350
EcoT38I GRGCYC 4 cut(s) 288, 843, 905, 1201
ErhI CCWWGG 3 cut(s) 844, 1761, 2446
Esp3I CGTCTC 1 cut(s) 2124
FaeI CATG 9 cut(s) 590, 848, 1117, 2000, 2352, 2362, 2508, 2593, 2642
FaqI GGGAC 5 cut(s) 679, 1759, 2162, 2376, 2412
FatI CATG 9 cut(s) 586, 844, 1113, 1996, 2348, 2358, 2504, 2589, 2638
FauI CCCGC 1 cut(s) 2188
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 2 cut(s) 450, 2523
Fnu4HI GCNGC 7 cut(s) 477, 570, 822, 930, 2456, 2653, 2656
FokI GGATG 6 cut(s) 314, 362, 951, 1694, 2081, 2627
FriOI GRGCYC 4 cut(s) 288, 843, 905, 1201
Fsp4HI GCNGC 7 cut(s) 477, 570, 822, 930, 2456, 2653, 2656
FspBI CTAG 5 cut(s) 119, 126, 482, 711, 2659
GluI GCNGC 7 cut(s) 477, 570, 822, 930, 2456, 2653, 2656
GsaI CCCAGC 1 cut(s) 1780
GsuI CTGGAG 3 cut(s) 488, 1721, 2303
HaeIII GGCC 6 cut(s) 686, 1402, 2063, 2167, 2394, 2600
HapII CCGG 1 cut(s) 1889
HgaI GACGC 1 cut(s) 2106
Hin1II CATG 9 cut(s) 590, 848, 1117, 2000, 2352, 2362, 2508, 2593, 2642
HincII GTYRAC 2 cut(s) 451, 1941
HindII GTYRAC 2 cut(s) 451, 1941
HindIII AAGCTT 2 cut(s) 152, 227
HinfI GANTC 9 cut(s) 122, 457, 728, 868, 1073, 1268, 1279, 1520, 1831
HpaII CCGG 1 cut(s) 1889
HphI GGTGA 4 cut(s) 669, 820, 1039, 1348
Hpy166II GTNNAC 4 cut(s) 451, 677, 1941, 2524
Hpy8I GTNNAC 4 cut(s) 451, 677, 1941, 2524
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 9 cut(s) 16, 67, 328, 419, 1229, 1391, 2165, 2314, 2533
HpyF10VI GCNNNNNNNGC 6 cut(s) 476, 740, 827, 1040, 2069, 2078
Hsp92II CATG 9 cut(s) 590, 848, 1117, 2000, 2352, 2362, 2508, 2593, 2642
Kpn2I TCCGGA 1 cut(s) 1888
Ksp22I TGATCA 1 cut(s) 273
LguI GCTCTTC 1 cut(s) 910
LmnI GCTCC 3 cut(s) 329, 606, 846
Lsp1109I GCAGC 5 cut(s) 488, 556, 808, 916, 2467
LweI GCATC 7 cut(s) 122, 1291, 1462, 1849, 2059, 2111, 2620
MaeI CTAG 5 cut(s) 119, 126, 482, 711, 2659
MaeIII GTNAC 8 cut(s) 35, 220, 316, 646, 850, 976, 1828, 2496
MbiI CCGCTC 2 cut(s) 427, 2377
MfeI CAATTG 2 cut(s) 1260, 1272
MflI RGATCY 3 cut(s) 492, 2287, 2606
MhlI GDGCHC 7 cut(s) 288, 843, 905, 1201, 1493, 1843, 2471
MlyI GAGTC 4 cut(s) 116, 737, 862, 1825
MmeI TCCRAC 3 cut(s) 371, 2167, 2258
Mph1103I ATGCAT 1 cut(s) 2350
MroI TCCGGA 1 cut(s) 1888
MroXI GAANNNNTTC 1 cut(s) 2194
MseI TTAA 7 cut(s) 434, 1550, 1749, 1770, 1968, 2220, 2427
MslI CAYNNNNRTG 4 cut(s) 380, 995, 1329, 2503
MspA1I CMGCKG 3 cut(s) 734, 2281, 2655
MspI CCGG 1 cut(s) 1889
MspR9I CCNGG 4 cut(s) 97, 173, 2139, 2396
MunI CAATTG 2 cut(s) 1260, 1272
Mva1269I GAATGC 2 cut(s) 1468, 2350
MvaI CCWGG 4 cut(s) 97, 173, 2139, 2396
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 6 cut(s) 476, 740, 827, 1040, 2069, 2078
NcoI CCATGG 1 cut(s) 844
NlaIII CATG 9 cut(s) 590, 848, 1117, 2000, 2352, 2362, 2508, 2593, 2642
NlaIV GGNNCC 4 cut(s) 406, 494, 2325, 2364
NmuCI GTSAC 7 cut(s) 35, 220, 316, 850, 976, 1828, 2496
NsiI ATGCAT 1 cut(s) 2350
NspI RCATGY 1 cut(s) 2508
NspV TTCGAA 1 cut(s) 2198
PagI TCATGA 1 cut(s) 2638
PciI ACATGT 1 cut(s) 2504
PciSI GCTCTTC 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 2 cut(s) 1468, 2350
PdmI GAANNNNTTC 1 cut(s) 2194
PfeI GAWTC 5 cut(s) 457, 1073, 1268, 1279, 1520
PflFI GACNNNGTC 1 cut(s) 1557
PflMI CCANNNNNTGG 3 cut(s) 592, 1387, 2240
PkrI GCNGC 7 cut(s) 478, 571, 823, 931, 2457, 2654, 2657
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 4 cut(s) 116, 736, 862, 1825
PpsI GAGTC 4 cut(s) 116, 736, 862, 1825
PpuMI RGGWCCY 2 cut(s) 2363, 2542
PscI ACATGT 1 cut(s) 2504
PshBI ATTAAT 1 cut(s) 1749
PsiI TTATAA 1 cut(s) 1443
Psp124BI GAGCTC 4 cut(s) 288, 843, 905, 1201
Psp5II RGGWCCY 2 cut(s) 2363, 2542
Psp6I CCWGG 4 cut(s) 95, 171, 2137, 2394
PspEI GGTNACC 1 cut(s) 646
PspFI CCCAGC 1 cut(s) 1776
PspGI CCWGG 4 cut(s) 95, 171, 2137, 2394
PspN4I GGNNCC 4 cut(s) 406, 494, 2325, 2364
PspPI GGNCC 4 cut(s) 685, 1400, 2363, 2542
PspPPI RGGWCCY 2 cut(s) 2363, 2542
PstI CTGCAG 1 cut(s) 2457
PstNI CAGNNNCTG 1 cut(s) 1931
PsuI RGATCY 3 cut(s) 492, 2287, 2606
PsyI GACNNNGTC 1 cut(s) 1557
PvuI CGATCG 1 cut(s) 34
PvuII CAGCTG 2 cut(s) 734, 2281
RsaI GTAC 7 cut(s) 50, 579, 1193, 1250, 1758, 2012, 2301
RsaNI GTAC 7 cut(s) 49, 578, 1192, 1249, 1757, 2011, 2300
RseI CAYNNNNRTG 4 cut(s) 380, 995, 1329, 2503
SacI GAGCTC 4 cut(s) 288, 843, 905, 1201
SalI GTCGAC 1 cut(s) 449
SapI GCTCTTC 1 cut(s) 910
SaqAI TTAA 7 cut(s) 434, 1550, 1749, 1770, 1968, 2220, 2427
SatI GCNGC 7 cut(s) 477, 570, 822, 930, 2456, 2653, 2656
Sau96I GGNCC 4 cut(s) 685, 1400, 2363, 2542
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 4 cut(s) 116, 737, 862, 1825
ScrFI CCNGG 4 cut(s) 97, 173, 2139, 2396
SduI GDGCHC 7 cut(s) 288, 843, 905, 1201, 1493, 1843, 2471
SfaNI GCATC 7 cut(s) 122, 1291, 1462, 1849, 2059, 2111, 2620
SfcI CTRYAG 1 cut(s) 2453
SfuI TTCGAA 1 cut(s) 2198
SinI GGWCC 2 cut(s) 2363, 2542
SmiMI CAYNNNNRTG 4 cut(s) 380, 995, 1329, 2503
SmlI CTYRAG 4 cut(s) 776, 1457, 2291, 2491
SmoI CTYRAG 4 cut(s) 776, 1457, 2291, 2491
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 8 cut(s) 299, 427, 724, 937, 2181, 2377, 2551, 2655
SspMI CTAG 5 cut(s) 119, 126, 482, 711, 2659
SstI GAGCTC 4 cut(s) 288, 843, 905, 1201
StyD4I CCNGG 4 cut(s) 95, 171, 2137, 2394
StyI CCWWGG 3 cut(s) 844, 1761, 2446
TaqI TCGA 5 cut(s) 69, 239, 450, 1474, 2198
TatI WGTACW 4 cut(s) 48, 1191, 1248, 2010
TauI GCSGC 1 cut(s) 2658
TfiI GAWTC 5 cut(s) 457, 1073, 1268, 1279, 1520
Tru1I TTAA 7 cut(s) 434, 1550, 1749, 1770, 1968, 2220, 2427
Tru9I TTAA 7 cut(s) 434, 1550, 1749, 1770, 1968, 2220, 2427
TseFI GTSAC 7 cut(s) 35, 220, 316, 850, 976, 1828, 2496
TseI GCWGC 6 cut(s) 476, 569, 821, 929, 2455, 2652
Tsp45I GTSAC 7 cut(s) 35, 220, 316, 850, 976, 1828, 2496
TspGWI ACGGA 5 cut(s) 45, 208, 218, 368, 662
Tth111I GACNNNGTC 1 cut(s) 1557
Van91I CCANNNNNTGG 3 cut(s) 592, 1387, 2240
VpaK11BI GGWCC 2 cut(s) 2363, 2542
VspI ATTAAT 1 cut(s) 1749
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 4 cut(s) 1352, 1502, 2087, 2192
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 1 cut(s) 2508
XmiI GTMKAC 2 cut(s) 450, 2523
XmnI GAANNNNTTC 1 cut(s) 2194
XspI CTAG 5 cut(s) 119, 126, 482, 711, 2659
ZrmI AGTACT 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.