pycom10g06040

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011365
Physical Location & Seq
Forward (+)
6798656 .. 6803153
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06040.6

Sequence Viewer

Length: 2352 bp
ATGAGTATTGTGACAAGCTCCCTGATGAAGGATATGAATAGTAAAACAGACATGTATCGGGCAAATGCCATCCGTGTCCTCTGTCGGATTACAGATGGAACCCTTCTTACCCAGATTGAGCGATATCTGAAACAAGCCATTGTTGATAAGAATCCAGTGGTTGCAAGTGCAGCTCTAGTCAGAGGGATCCATCTGCTCCAGACTAACCCAGAGATTGTCAAAAGATGGAGCAATGAGGTTCAGGAAGCTGTCCAGTCAAGAGCAGCCCTTGTACAGTTCCATGCACTGGCTTTACTTCATCAGATACGACAGAATGACCGTCTTGCTGTTAGCAAGTTGGTTTCCAGCTTGACAAAGGGGAATGTCCGCTCTCCATTGGCCCAGTGCCTTTTGATTCGTTATACTAGTCAGGTCCGCGAGTCTGGAAATGCACAAGCGGGCGACCGTCCCTTTTACGATTATCTTGAGGCTTGTCTTCGCCACAAAGCAGAGATGGTGATTTTGGAGGCTGCTAGAGCAATTACAGAGCTCCATGGTGTGACCCCTAGAGAACTTACTCCAGCAATCACTGTTCTGCAGCTATTCCTAAGCTCTTCGAAACCAGTTCTAAGATTTGCTGCTGTCCGCACCTTGAACAAGGTGGCAATGACACATCCAGTGCCTGTCACAAACTGCAACATTGATATGGAGAGTTTGATTTCTGACCAGAACAGGAGCATTGCCACACTTGCAATCACAACACTTCTGAAGACAGGAAATGAATCAAGTGTGGATCGCTTGATGAAGCAGATAACTAATTTCATGTCGGATATCGCTGACGAGTTCAAAATTGTTGTTGTGGAAGCCATAAGATCGTTGTGTTTGAAATTTCCTTTGAAGTTCAGAGCTCTGATGAACTTTTTGAGCAACATTCTCAGAGAAGAAGGTGGGTTTGAGTACAAAAAGGCAATTGTTGATTCAATTGTGATTCTCATTAGAGATATTCCTGATGCAAAAGAAAGTGGCTTGCTTCATCTCTGCGAGTTCATCGAAGATTGTGAATTCACCTATCTTTCTACACAGATACTCCACTTTTTGGGCATTGAAGGGCCAAAAACCTCTGATCCAAGCAAATATATAAGGTACATATATAATCGAGTACATCTTGAGAATGCCACTGTTCGTGCGAGTGCAGTTAGTACATTGGCAAAATTTGGTGCTATGGTTGATTCATTGAAGCCGCGAGTGTTTATTCTGCTGCGACGATGTCTCTTTGACAGTGATGATGAGGTTCGTGATAGGGCAACACTCTATCTAAATACGCTTGGAGGAGATGGTTCAGTTGTTGAGACTGACAATGATGTAAAGGACTTCCTTTTTGGGTCTCTGGATGTCCCACTTGTCAATCTGGAGACAAGTTTGAAGAATTATGAGGCTTCGGAAGAACCATTTGACATTAATTCTGTACCCAAGGAGGTTAAGTCACAGCCACTTGCTGAGAAGAAAGCTCAAAGTAAAAAGCCAACTGGTCTTGGTGCTCCTCCAACTGGCCCTGCATCCACAGTTGCTGCTTATGAGGCGTTGCTTTCCTCTATTCCGGAGTTTTCAAACTTTGGGAAACTATTCAAGTCCTCAGCACCTGTGGAGTTAACAGAGCCAGAAACTGAATATGCAGTTAATGTTGTCAAGCACATTTTTGATAGCCATGTTGTGTTCCAGTACAACTGCACCAATACCATTCCTGAGCAATTACTGGAAGATGTCATTGTCGTGGTTGATGCTTCAGAAGCTGAGGAATTTTCTGAAGTGGCATCCAGGTCTCTCAGGTCTCTTCCATATGATACACCTGGACAAACCTTTTTGGCATTTGAGAAGCCAGAAGGTGTCCCTGCAGTTGGAAAATTTACAAACTTGTTGAGGTTCATTGTTAAAGAGGTTGATCGATCCACTGGTGAGGCAGAGGAAGATGGTGTGGAAGATGAATACCAGCTGGAAGATCTTGAGGTTGTCGCTGCAGATTACATTTTGAAGGTTCCAGTCTTCAATTTCAAGAATGCTTGGGATAGCATGGGTGCTGATTTTGAGCGGGTAGATGAATATGGCCTGGGGCCAAGGGAGAACTTGACTGAAGCTGTTAATGCTGTCATTAACCTCCTTGGAATGCAGCCCTGTGAGGGCACAGAGGTCGTTGCCAGCAACTCAAGGTCACACACATGTGTATTGTCCGGTGTGTACATAGGCAATGTGAAGGTTCTTGTGCGGTTGTTATTTGGAATTGACAGTTCATCGAAGGAGGTTGCGATGAAACTTACTGTCAGATCGGAGGATGGAGCTGTCAGTGATGCCATTCATGAGATTGTGGCCAGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N COP-gamma_platf Coatomer_g_Cpla

Protein Analysis

784

Amino Acids

86.65

Weight (kDa)

5.21

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34450
fragaria_vesca FvH4_2g08660 FvH4_2g08660 FvH4_2g08660
malus_domestica MD05G1067500.v1.1 MD10G1077400.v1.1
prunus_persica Prupe.8G098200_v2.0.a1
pyrus_communis pycom05g05860 pycom10g06040
rosa_chinensis RchiOBHm_Chr6g0264051
rosa_laevigata RLG00000014293
rosa_multiflora Rmu_sc0006339.1_g000005
rosa_roxburghii Rroxscaffold_176G00431390 Rroxscaffold_7G00203630
rosa_rugosa Rorug06G0007600
rosa_samantha Rh6AG128800 Rh6BG124900 Rh6CG123600 Rh6DG110600
rosa_wichuraiana Rw6G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 286, 1075
AccBSI CCGCTC 2 cut(s) 369, 2065
AccII CGCG 2 cut(s) 417, 1222
AccIII TCCGGA 1 cut(s) 1576
AciI CCGC 8 cut(s) 367, 415, 437, 625, 1220, 2065, 2239, 2346
AclWI GGATC 5 cut(s) 181, 194, 780, 1097, 1917
AcoI YGGCCR 1 cut(s) 2340
AcsI RAATTY 5 cut(s) 866, 1040, 1190, 1775, 1880
AcuI CTGAAG 4 cut(s) 767, 1746, 1803, 2127
AfaI GTAC 8 cut(s) 273, 938, 1124, 1140, 1180, 1446, 1700, 2213
AfiI CCNNNNNNNGG 6 cut(s) 28, 286, 1075, 1526, 1874, 2153
AflIII ACRYGT 2 cut(s) 51, 2192
AhlI ACTAGT 1 cut(s) 404
AjnI CCWGG 3 cut(s) 1793, 1825, 2082
AleI CACNNNNGTG 1 cut(s) 2193
Alw21I GWGCWC 3 cut(s) 531, 889, 1519
Alw26I GTCTC 6 cut(s) 1253, 1322, 1368, 1385, 1803, 1812
AlwI GGATC 5 cut(s) 181, 194, 780, 1097, 1917
AlwNI CAGNNNCTG 5 cut(s) 662, 1547, 1619, 1643, 1769
Aor13HI TCCGGA 1 cut(s) 1576
AoxI GGCC 6 cut(s) 378, 1088, 1528, 2080, 2087, 2340
ApeKI GCWGC 9 cut(s) 170, 263, 509, 577, 617, 1237, 1547, 1991, 2143
ApoI RAATTY 5 cut(s) 866, 1040, 1190, 1775, 1880
ArsI GACNNNNNNTTYG 2 cut(s) 1340, 1372
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 1 cut(s) 1601
AspS9I GGNCC 5 cut(s) 379, 412, 1088, 1529, 2087
AsuHPI GGTGA 3 cut(s) 508, 1036, 1943
AsuII TTCGAA 1 cut(s) 596
AvaII GGWCC 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 2159
BalI TGGCCA 1 cut(s) 2342
BamHI GGATCC 1 cut(s) 186
BanII GRGCYC 2 cut(s) 531, 889
BbsI GAAGAC 3 cut(s) 467, 755, 2011
Bbv12I GWGCWC 3 cut(s) 531, 889, 1519
BbvCI CCTCAGC 2 cut(s) 1612, 1770
BbvI GCAGC 9 cut(s) 182, 275, 496, 589, 604, 1224, 1534, 1978, 2155
BccI CCATC 8 cut(s) 77, 89, 198, 219, 487, 1307, 1940, 2300
BcgI CGANNNNNNTGC 2 cut(s) 2146, 2180
BciT130I CCWGG 3 cut(s) 1795, 1827, 2084
BcoDI GTCTC 6 cut(s) 1253, 1322, 1368, 1385, 1803, 1812
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 5 cut(s) 176, 405, 513, 546, 2350
BfmI CTRYAG 3 cut(s) 575, 1869, 1992
BglII AGATCT 1 cut(s) 1975
Bme1390I CCNGG 3 cut(s) 1795, 1827, 2084
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 5 cut(s) 379, 412, 1088, 1529, 2087
BmiI GGNNCC 4 cut(s) 100, 188, 2013, 2088
BmrFI CCNGG 3 cut(s) 1795, 1827, 2084
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 5 cut(s) 979, 1544, 1747, 1799, 2311
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 3 cut(s) 467, 755, 2011
BpmI CTGGAG 3 cut(s) 182, 543, 1409
Bpu10I CCTNAGC 4 cut(s) 587, 1612, 1722, 1770
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 4 cut(s) 485, 1166, 2000, 2164
Bsa29I ATCGAT 1 cut(s) 1921
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 3 cut(s) 1368, 1803, 1812
BsaJI CCNNGG 5 cut(s) 532, 1449, 2083, 2090, 2134
BsaWI WCCGGW 2 cut(s) 1576, 2204
BsaXI ACNNNNNCTCC 4 cut(s) 1050, 1080, 1445, 1475
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 286, 1075, 1526, 1874, 2153
Bse3DI GCAATG 4 cut(s) 238, 651, 717, 2227
Bse8I GATNNNNATC 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 1576
BseBI CCWGG 3 cut(s) 1795, 1827, 2084
BseCI ATCGAT 1 cut(s) 1921
BseDI CCNNGG 5 cut(s) 532, 1449, 2083, 2090, 2134
BseGI GGATG 6 cut(s) 69, 652, 1375, 1535, 1790, 2311
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 6 cut(s) 28, 286, 1075, 1526, 1874, 2153
BseMI GCAATG 4 cut(s) 238, 651, 717, 2227
BseMII CTCAG 6 cut(s) 928, 1467, 1626, 1713, 1761, 1816
BseRI GAGGAG 2 cut(s) 1323, 1509
BseSI GKGCMC 1 cut(s) 2159
BseXI GCAGC 9 cut(s) 182, 275, 496, 589, 604, 1224, 1534, 1978, 2155
BsgI GTGCAG 3 cut(s) 189, 1191, 1690
Bsh1236I CGCG 2 cut(s) 417, 1222
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 6 cut(s) 380, 1090, 1530, 2082, 2089, 2342
BshVI ATCGAT 1 cut(s) 1921
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 3 cut(s) 531, 889, 1519
BsiSI CCGG 2 cut(s) 1577, 2205
BslFI GGGAC 3 cut(s) 432, 1358, 1850
BslI CCNNNNNNNGG 6 cut(s) 28, 286, 1075, 1526, 1874, 2153
BsmAI GTCTC 6 cut(s) 1253, 1322, 1368, 1385, 1803, 1812
BsmFI GGGAC 3 cut(s) 432, 1358, 1850
BsmI GAATGC 3 cut(s) 1156, 2038, 2145
BsnI GGCC 6 cut(s) 380, 1090, 1530, 2082, 2089, 2342
Bso31I GGTCTC 3 cut(s) 1368, 1803, 1812
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 4 cut(s) 531, 889, 1519, 2159
Bsp13I TCCGGA 1 cut(s) 1576
Bsp1407I TGTACA 2 cut(s) 271, 2211
Bsp143I GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
Bsp19I CCATGG 1 cut(s) 532
BspACI CCGC 8 cut(s) 367, 415, 437, 625, 1220, 2065, 2239, 2346
BspANI GGCC 6 cut(s) 380, 1090, 1530, 2082, 2089, 2342
BspCNI CTCAG 6 cut(s) 927, 1468, 1625, 1714, 1762, 1815
BspDI ATCGAT 1 cut(s) 1921
BspEI TCCGGA 1 cut(s) 1576
BspFNI CGCG 2 cut(s) 417, 1222
BspHI TCATGA 1 cut(s) 2329
BspLI GGNNCC 4 cut(s) 100, 188, 2013, 2088
BspMAI CTGCAG 3 cut(s) 579, 1873, 1996
BspPI GGATC 5 cut(s) 181, 194, 780, 1097, 1917
BspQI GCTCTTC 1 cut(s) 598
BspT104I TTCGAA 1 cut(s) 596
BspTNI GGTCTC 3 cut(s) 1368, 1803, 1812
BsrBI CCGCTC 2 cut(s) 369, 2065
BsrDI GCAATG 4 cut(s) 238, 651, 717, 2227
BsrGI TGTACA 2 cut(s) 271, 2211
BssECI CCNNGG 5 cut(s) 532, 1449, 2083, 2090, 2134
BssMI GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
BssT1I CCWWGG 4 cut(s) 532, 1449, 2090, 2134
Bst2UI CCWGG 3 cut(s) 1795, 1827, 2084
Bst4CI ACNGT 9 cut(s) 276, 320, 446, 571, 1159, 1259, 1543, 2261, 2293
Bst6I CTCTTC 2 cut(s) 598, 1815
BstAUI TGTACA 2 cut(s) 271, 2211
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 4 cut(s) 439, 1007, 2173, 2344
BstDEI CTNAG 8 cut(s) 587, 608, 914, 1476, 1612, 1722, 1770, 1802
BstDSI CCRYGG 1 cut(s) 532
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 6 cut(s) 69, 652, 1375, 1535, 1790, 2311
BstFNI CGCG 2 cut(s) 417, 1222
BstKTI GATC 8 cut(s) 189, 775, 854, 1105, 1921, 1925, 1978, 2300
BstMAI GTCTC 6 cut(s) 1253, 1322, 1368, 1385, 1803, 1812
BstMBI GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 6 cut(s) 170, 515, 728, 1556, 1766, 2117
BstNI CCWGG 3 cut(s) 1795, 1827, 2084
BstNSI RCATGY 2 cut(s) 55, 2196
BstSCI CCNGG 3 cut(s) 1793, 1825, 2082
BstSFI CTRYAG 3 cut(s) 575, 1869, 1992
BstSLI GKGCMC 1 cut(s) 2159
BstUI CGCG 2 cut(s) 417, 1222
BstV1I GCAGC 9 cut(s) 182, 275, 496, 589, 604, 1224, 1534, 1978, 2155
BstV2I GAAGAC 3 cut(s) 467, 755, 2011
BstX2I RGATCY 2 cut(s) 186, 1975
BstYI RGATCY 2 cut(s) 186, 1975
Bsu15I ATCGAT 1 cut(s) 1921
BsuRI GGCC 6 cut(s) 380, 1090, 1530, 2082, 2089, 2342
BsuTUI ATCGAT 1 cut(s) 1921
BtgI CCRYGG 1 cut(s) 532
BtgZI GCGATG 1 cut(s) 2294
BtsCI GGATG 6 cut(s) 69, 652, 1375, 1535, 1790, 2311
BtsIMutI CAGTG 9 cut(s) 162, 284, 389, 567, 663, 1155, 1264, 1926, 2323
Cac8I GCNNGC 4 cut(s) 439, 1007, 2173, 2344
CaiI CAGNNNCTG 5 cut(s) 662, 1547, 1619, 1643, 1769
CciI TCATGA 1 cut(s) 2329
Cfr13I GGNCC 5 cut(s) 379, 412, 1088, 1529, 2087
ClaI ATCGAT 1 cut(s) 1921
Csp6I GTAC 8 cut(s) 272, 937, 1123, 1139, 1179, 1445, 1699, 2212
CviAII CATG 8 cut(s) 52, 281, 533, 802, 1685, 2047, 2193, 2330
CviQI GTAC 8 cut(s) 272, 937, 1123, 1139, 1179, 1445, 1699, 2212
DdeI CTNAG 8 cut(s) 587, 608, 914, 1476, 1612, 1722, 1770, 1802
DpnI GATC 8 cut(s) 188, 774, 853, 1104, 1920, 1924, 1977, 2299
DpnII GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
EaeI YGGCCR 1 cut(s) 2340
Eam1104I CTCTTC 2 cut(s) 598, 1815
EarI CTCTTC 2 cut(s) 598, 1815
Ecl136II GAGCTC 2 cut(s) 529, 887
Eco130I CCWWGG 4 cut(s) 532, 1449, 2090, 2134
Eco24I GRGCYC 2 cut(s) 531, 889
Eco31I GGTCTC 3 cut(s) 1368, 1803, 1812
Eco32I GATATC 2 cut(s) 125, 811
Eco47I GGWCC 1 cut(s) 412
Eco53kI GAGCTC 2 cut(s) 529, 887
Eco57I CTGAAG 4 cut(s) 767, 1746, 1803, 2127
EcoICRI GAGCTC 2 cut(s) 529, 887
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 1040
EcoRII CCWGG 3 cut(s) 1793, 1825, 2082
EcoRV GATATC 2 cut(s) 125, 811
EcoT14I CCWWGG 4 cut(s) 532, 1449, 2090, 2134
EcoT38I GRGCYC 2 cut(s) 531, 889
ErhI CCWWGG 4 cut(s) 532, 1449, 2090, 2134
FaeI CATG 8 cut(s) 55, 284, 536, 805, 1688, 2050, 2196, 2333
FaqI GGGAC 3 cut(s) 432, 1358, 1850
FatI CATG 8 cut(s) 51, 280, 532, 801, 1684, 2046, 2192, 2329
FauI CCCGC 2 cut(s) 430, 2058
FauNDI CATATG 1 cut(s) 1816
FokI GGATG 6 cut(s) 56, 639, 1382, 1522, 1777, 2318
FriOI GRGCYC 2 cut(s) 531, 889
FspBI CTAG 5 cut(s) 176, 405, 513, 546, 2350
GsuI CTGGAG 3 cut(s) 182, 543, 1409
HaeIII GGCC 6 cut(s) 380, 1090, 1530, 2082, 2089, 2342
HapII CCGG 2 cut(s) 1577, 2205
Hin1II CATG 8 cut(s) 55, 284, 536, 805, 1688, 2050, 2196, 2333
HincII GTYRAC 1 cut(s) 1629
HindII GTYRAC 1 cut(s) 1629
HinfI GANTC 7 cut(s) 151, 394, 419, 761, 956, 967, 1208
HpaI GTTAAC 1 cut(s) 1629
HpaII CCGG 2 cut(s) 1577, 2205
HphI GGTGA 3 cut(s) 508, 1036, 1943
Hpy166II GTNNAC 2 cut(s) 1629, 2212
Hpy8I GTNNAC 2 cut(s) 1629, 2212
Hpy99I CGWCG 1 cut(s) 1245
HpyAV CCTTC 8 cut(s) 22, 113, 917, 1079, 1853, 2002, 2221, 2263
HpyCH4III ACNGT 9 cut(s) 276, 320, 446, 571, 1159, 1259, 1543, 2261, 2293
HpyF10VI GCNNNNNNNGC 6 cut(s) 170, 515, 728, 1556, 1766, 2117
HpyF3I CTNAG 8 cut(s) 587, 608, 914, 1476, 1612, 1722, 1770, 1802
Hsp92II CATG 8 cut(s) 55, 284, 536, 805, 1688, 2050, 2196, 2333
Kpn2I TCCGGA 1 cut(s) 1576
KspAI GTTAAC 1 cut(s) 1629
Kzo9I GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 7 cut(s) 23, 201, 228, 534, 714, 1522, 2309
Lsp1109I GCAGC 9 cut(s) 182, 275, 496, 589, 604, 1224, 1534, 1978, 2155
LweI GCATC 5 cut(s) 979, 1544, 1747, 1799, 2311
MaeI CTAG 5 cut(s) 176, 405, 513, 546, 2350
MaeIII GTNAC 5 cut(s) 10, 538, 664, 1461, 2184
MalI GATC 8 cut(s) 188, 774, 853, 1104, 1920, 1924, 1977, 2299
MbiI CCGCTC 2 cut(s) 369, 2065
MboI GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
MfeI CAATTG 2 cut(s) 948, 960
MflI RGATCY 2 cut(s) 186, 1975
MhlI GDGCHC 4 cut(s) 531, 889, 1519, 2159
MlsI TGGCCA 1 cut(s) 2342
MluNI TGGCCA 1 cut(s) 2342
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 4 cut(s) 65, 786, 1547, 1855
Mox20I TGGCCA 1 cut(s) 2342
MroI TCCGGA 1 cut(s) 1576
MroXI GAANNNNTTC 1 cut(s) 1601
MscI TGGCCA 1 cut(s) 2342
MseI TTAA 7 cut(s) 1437, 1458, 1628, 1656, 1908, 2115, 2127
MslI CAYNNNNRTG 4 cut(s) 683, 1748, 2191, 2193
Msp20I TGGCCA 1 cut(s) 2342
MspA1I CMGCKG 2 cut(s) 1969, 2346
MspI CCGG 2 cut(s) 1577, 2205
MspR9I CCNGG 3 cut(s) 1795, 1827, 2084
MunI CAATTG 2 cut(s) 948, 960
Mva1269I GAATGC 3 cut(s) 1156, 2038, 2145
MvaI CCWGG 3 cut(s) 1795, 1827, 2084
MvnI CGCG 2 cut(s) 417, 1222
MwoI GCNNNNNNNGC 6 cut(s) 170, 515, 728, 1556, 1766, 2117
NcoI CCATGG 1 cut(s) 532
NdeI CATATG 1 cut(s) 1816
NdeII GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
NlaIII CATG 8 cut(s) 55, 284, 536, 805, 1688, 2050, 2196, 2333
NlaIV GGNNCC 4 cut(s) 100, 188, 2013, 2088
NmuCI GTSAC 5 cut(s) 10, 538, 664, 1461, 2184
NspI RCATGY 2 cut(s) 55, 2196
NspV TTCGAA 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 2193
PagI TCATGA 1 cut(s) 2329
PciI ACATGT 2 cut(s) 51, 2192
PciSI GCTCTTC 1 cut(s) 598
PctI GAATGC 3 cut(s) 1156, 2038, 2145
PdmI GAANNNNTTC 1 cut(s) 1601
PfeI GAWTC 6 cut(s) 151, 394, 761, 956, 967, 1208
PflFI GACNNNGTC 1 cut(s) 1245
PflMI CCANNNNNTGG 2 cut(s) 286, 1075
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PscI ACATGT 2 cut(s) 51, 2192
PshBI ATTAAT 1 cut(s) 1437
Psp124BI GAGCTC 2 cut(s) 531, 889
Psp6I CCWGG 3 cut(s) 1793, 1825, 2082
PspGI CCWGG 3 cut(s) 1793, 1825, 2082
PspN4I GGNNCC 4 cut(s) 100, 188, 2013, 2088
PspPI GGNCC 5 cut(s) 379, 412, 1088, 1529, 2087
PsrI GAACNNNNNNTAC 2 cut(s) 91, 123
PstI CTGCAG 3 cut(s) 579, 1873, 1996
PstNI CAGNNNCTG 5 cut(s) 662, 1547, 1619, 1643, 1769
PsuI RGATCY 2 cut(s) 186, 1975
PsyI GACNNNGTC 1 cut(s) 1245
PvuII CAGCTG 1 cut(s) 1969
RsaI GTAC 8 cut(s) 273, 938, 1124, 1140, 1180, 1446, 1700, 2213
RsaNI GTAC 8 cut(s) 272, 937, 1123, 1139, 1179, 1445, 1699, 2212
RseI CAYNNNNRTG 4 cut(s) 683, 1748, 2191, 2193
SacI GAGCTC 2 cut(s) 531, 889
SapI GCTCTTC 1 cut(s) 598
SaqAI TTAA 7 cut(s) 1437, 1458, 1628, 1656, 1908, 2115, 2127
Sau3AI GATC 8 cut(s) 186, 772, 851, 1102, 1918, 1922, 1975, 2297
Sau96I GGNCC 5 cut(s) 379, 412, 1088, 1529, 2087
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 3 cut(s) 1795, 1827, 2084
SduI GDGCHC 4 cut(s) 531, 889, 1519, 2159
SfaNI GCATC 5 cut(s) 979, 1544, 1747, 1799, 2311
SfcI CTRYAG 3 cut(s) 575, 1869, 1992
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 4 cut(s) 683, 1748, 2191, 2193
SmlI CTYRAG 4 cut(s) 464, 1145, 1979, 2179
SmoI CTYRAG 4 cut(s) 464, 1145, 1979, 2179
SpeI ACTAGT 1 cut(s) 404
SsiI CCGC 8 cut(s) 367, 415, 437, 625, 1220, 2065, 2239, 2346
SspMI CTAG 5 cut(s) 176, 405, 513, 546, 2350
SstI GAGCTC 2 cut(s) 531, 889
StyD4I CCNGG 3 cut(s) 1793, 1825, 2082
StyI CCWWGG 4 cut(s) 532, 1449, 2090, 2134
TaaI ACNGT 9 cut(s) 276, 320, 446, 571, 1159, 1259, 1543, 2261, 2293
TaqI TCGA 5 cut(s) 596, 1029, 1135, 1921, 2267
TatI WGTACW 6 cut(s) 271, 936, 1138, 1178, 1698, 2211
TauI GCSGC 2 cut(s) 1222, 2349
TfiI GAWTC 6 cut(s) 151, 394, 761, 956, 967, 1208
Tru1I TTAA 7 cut(s) 1437, 1458, 1628, 1656, 1908, 2115, 2127
Tru9I TTAA 7 cut(s) 1437, 1458, 1628, 1656, 1908, 2115, 2127
TscAI CASTG 9 cut(s) 162, 291, 389, 574, 663, 1162, 1264, 1933, 2323
TseFI GTSAC 5 cut(s) 10, 538, 664, 1461, 2184
TseI GCWGC 9 cut(s) 170, 263, 509, 577, 617, 1237, 1547, 1991, 2143
Tsp45I GTSAC 5 cut(s) 10, 538, 664, 1461, 2184
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 9 cut(s) 162, 291, 389, 574, 663, 1162, 1264, 1933, 2323
Tth111I GACNNNGTC 1 cut(s) 1245
Van91I CCANNNNNTGG 2 cut(s) 286, 1075
VpaK11BI GGWCC 1 cut(s) 412
VspI ATTAAT 1 cut(s) 1437
XagI CCTNNNNNAGG 1 cut(s) 26
XapI RAATTY 5 cut(s) 866, 1040, 1190, 1775, 1880
XceI RCATGY 2 cut(s) 55, 2196
XmnI GAANNNNTTC 1 cut(s) 1601
XspI CTAG 5 cut(s) 176, 405, 513, 546, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.